Dissertations / Theses on the topic 'Reverse translation'

My PhD thesis proposes to look at film adaptation through a concept derived from ekphrastic discourse in order to delineate a critical space, or ‘ekphrastic third space’, opened up by the process of adaptation. It raises questions about the semiotic dialogue between (moving) image and text, cinema and literature, and how that dialogue is enriched by reconfiguring traditional notions of ekphrasis as ‘reverse ekphrasis’. The chosen case studies - film adaptation of classical myths - reconnect with the origins of ekphrasis as a rhetorical figure, in an attempt to link it with cinema's 'mythical' dimension. The concept of reverse ekphrasis is mediated through a hermeneutic theory of translation which, 1 argue, offers a way of countering overly instrumentalist or transpositional, semiotic readings of film adaptations. The hermeneutic model allows the critic to see all texts as inherently unstable and dialectical; thus opening the way to seeing film adaptations of classical myths as a way of revealing and/or problematising the conditions of production. The ekphrastic third space is therefore not defined by its polarity but by the interlacing occurring in-between; its premise is founded on an unpredictable process’vyhich adjusts itself as a territory for creativity and critical thinking, not on a finality based on a logic of containment. The image-text relationship is thus envisaged not only as a relationship between the said and the seen but between the unsaid and the unseen. The outcome of such an approach is twofold: the process is both mechanical and organic, hermeneutic and poetical, which implies a constant concern for the ambivalence of signs. In that sense, the workings, or illogical ‘logic’, of reverse ekphrasis concur with a cinema of signs that opens up a discursive space on the transformative process from words into moving images and on its conditions of production.

Enteroviruses belong to the Picornaviridae family and are small icosahedral viruses with RNA genomes of positive polarity, containing a single open reading frame. They mostly cause mild or asymptomatic infections, but also a wide array of diseases including: poliomyelitis, encephalitis, gastroenteritis, aseptic meningitis, myocarditis, hand-foot-and-mouth disease, hepatitis and respiratory diseases, ranging from severe infections to the common cold. The projects described in this thesis have been carried out through reverse genetic studies of Enterovirus B and Rhinovirus C.                   In Papers I and II, a cassette vector was used to study recombination and translation of the RNA genome. It was found that the non-structural coding region could replicate when combined with the structural protein-coding region of other viruses of the same species. Furthermore, the genome could be translated and replicated without the presence of the structural protein-coding region. Moreover, it was found that when two additional nucleotides were introduced, shifting the reading frame, the virus could revert to the original reading frame, restoring efficient replication. In Paper III, a vector containing the genome of echovirus 5 was altered to produce an authentic 5’end of the in vitro transcribed RNA, which increased efficiency of replication initiation 20 times. This result is important, as it may lead to more efficient oncolytic virotherapy. An authentic 5’end was further used in Paper IV, where replication of Rhinovirus C in cell lines was attempted. Although passaging of the virus was unsuccessful, the genome was replicated and cytopathic effect induced after transfection. The restriction of efficient replication was therefore hypothesized to lie in the attachment and entry stages of the replication cycle. In Paper V, a cytolytic virus was found to have almost 10 times larger impact on gene expression of the host cell than a non-cytolytic variant. Furthermore, the lytic virus was found to build up inside the host cell, while the non-cytolytic virus was efficiently released.                   As a whole, this thesis has contributed to a deeper understanding of replication of enteroviruses, which may prove important in development of novel vaccines, antiviral agents and oncolytic virotherapies.

Much research exists on cancel culture and cultural gatekeeping. However, there is little research on more recent examples of cancel culture stemming from the Netherlands. The current study sought to examine how active Twitter users have responded to what I have titled, the Rijneveld translation controversy on Twitter. This controversy involves questions of racism or reverse racism after a Dutch White translator, Marieke Lucas Rijneveld, reversed their decision to translate works of the African-American writer, Amanda Gorman after receiving much backlash from the public. This was followed by debates on Twitter causing an uproar. Therefore, the objective of this study was to examine the different issue- frames tweeted about by active Twitter users through a qualitative content analysis. In order to inquire into the opinions addressed at various stages of the controversy, tweets were collected over the course of three different time periods. A general observation was that a majority of Twitter users were upset by the pushback Rijneveld received and even regarded the situation as an example of “reverse racism” and radical wokeism. Moreover, several different actors/stakeholders were targeted or “called-out” by the “Twitter mob,” including the Dutch journalist, Janice Deul who led part of the pushback against Rijneveld. These issues are substantially less about the art and craft of translation and reflect a broader societal issue that Twitter users felt a need to address through this controversy.

Negli ultimi anni, l'industria del videogioco ha raggiunto delle dimensioni notevoli, generando nel 2016 91 miliardi di dollari a livello mondiale. La localizzazione dei videogiochi è diventata essenziale per far fronte ai costi di sviluppo sempre maggiori di questi prodotti, ricoprendo un ruolo rilevante nella loro commercializzazione in mercati esteri. Al contempo, l'adattamento di un videogioco presenta caratteristiche distinte dalla localizzazione di altri software. Il progetto trattato nel presente elaborato, ossia la localizzazione del videogioco Undertale attraverso la modifica diretta del codice sorgente, offre uno sguardo al flusso di lavoro tipico e alle problematiche caratteristiche della localizzazione professionale dei videogiochi, inoltrandosi nei dettagli tecnici del processo. L'elaborato è suddiviso in tre capitoli. Nel capitolo 1 viene analizzata la localizzazione professionale dei videogiochi, il flusso di lavoro tipico e le problematiche più frequenti, come il limite di caratteri, la presenza di elementi grafici impossibili da modificare o di elementi culturali che potrebbero necessitare di adattamento o censura. Nel capitolo 2 viene trattato il fenomeno della localizzazione amatoriale, includendo i risultati di un sondaggio condotto tra utenti italiani per indagare sulla loro percezione della qualità generale delle localizzazioni ufficiali e di alcune strategie traduttive. Nel capitolo 3 sono presentati i dettagli del progetto di localizzazione di Undertale: dalle caratteristiche del codice sorgente, all'identificazione delle stringhe e al reverse engineering dei metacaratteri, giungendo quindi al workflow seguito per la traduzione e il testing del gioco localizzato. Nel capitolo 4 trova spazio un commento alla localizzazione, con l'analisi delle problematiche tecniche e linguistico/testuali affrontate. Infine, vengono discussi i risultati raggiunti, sottolineando potenzialità e limiti di progetti di localizzazione simili.

This experimental study addresses one of the least explored audiovisual modes: reverse subtitling (L1 audio, L2 subtitles). Specifically, it investigates the effects of different translational choices on learners’ noticing and memorisation of lexical items and grammatical structures. The participants were English (L1) native speakers learning Italian (L2) at an upper-intermediate level (CEFR B2). Formal similarity (literal transfer) and discrepancy (non-literal transfer) between L1 and L2 were compared to establish if and how they affect the learners during subtitle processing and recall. Does one of the two translation conditions yield a better recall rate in a verbatim memory post-test? This constitutes the main research question addressed in this study. The main hypothesis was that there would be a difference in recall by translation condition, with formal equivalence having a facilitative effect on memory and literal subtitles therefore being more accurately recalled by learners. To determine how the different subtitle translations were processed, attention allocation and noticing were investigated through triangulation of eye-tracking, the recall post-test and an open questionnaire, which allowed the subjects’ thought processes to also be recorded. Subtitle-specific variables such as corpus frequency and linguistic category (lexicon vs. syntax) were also analysed. While participants watched the reversely subtitled clip, their eye behaviour was recorded using a Tobii X120 eye-tracker. After watching, participants answered the recall post-test followed by the open questionnaire and took part in a working memory control test. Translation condition was found to influence recall, with literal translations yielding superior recall performance than non-literal ones. The data also showed that participants did notice a translation discrepancy. Eye-tracking findings reveal a complex relationship between language elaboration and memory, whereby comparable amounts of visual attention to two subtitle versions can result in significantly different recall. Moreover, considerable insights were drawn from the open questionnaire, indicating that qualitative data can provide a richer picture of processing and memory attainment and should more regularly support experimental studies. The results demonstrate that subtitle-specific factors like translation can indeed influence the viewer and should therefore be taken into consideration in the design of future subtitle reception studies. The mnemonic potential of reverse subtitles for foreign language learning is also confirmed, strongly suggesting that this subtitling mode should be reconsidered as a valuable tool in language learning and deserves a place in the foreign language classroom. Future research could build on this study by using a larger sample size and more advanced statistical techniques, such as multilevel modelling. The results obtained highlight the complexity of the language faculty and call for additional reception studies where more fine-grained analyses further assess the effects of translation during the consumption of subtitled material. For instance, using a higher-frequency eye-tracker and considering more eye movement measures in the future will provide more precise insights in the reading process, enabling deeper understanding of information processing and memory retention, both crucial aspects in the development of foreign language skills.

This master thesis covers re-engineering methods of legacy systems. Legacy system is an old system, which is hardly compliant with modern technologies and used only because it has become an integral part of organization business process support during the long period of its maintenance. These systems are large, monolithic and difficult to modify, and cost and risk of their replacement are difficult to predict. The science of software engineering offers an incremental modernization of information systems applying the re-engineering of legacy software. The main goal of software re-engineering is to transform the software in the way, it would become easier to understand, maintain and re-use, at the same time preserving its useful, time trusted functions. The main re-engineering methods are source code translation, reverse engineering and data re-engineering. This thesis covers the analysis of these methods, which was made during the re-engineering of wood production and sales accounting system. The adoption and realization time rate of every method was examined. The influence of the applied re-engineering methods to the system reliability, efficiency, usability and other quality metrics is given.

Dysregulation of the phosphotidylinositol-3-kinase (PI3-kinase)/AKT signalling pathway is one of the most frequent mutational events in various human malignancies, including ovarian cancer. Increased PI3K/AKT activity in malignant cells is associated with resistance to chemotherapeutic agents. GSK2141795 is a potent pan-AKT kinase inhibitor, being developed for ovarian and other cancers. The aim of the present study was to evaluate the ability of GSK2141795 to restore platinum sensitivity to platinum-resistant ovarian cancer cell lines. Effective pharmacodynamic (PD) biomarkers are critical for the implementation and assessment of targeted therapeutics. The second aim of this study was to explore the utility of using fluoro-deoxy-glucose positron emission tomography (FDG-PET) imaging as a PD marker by correlating changes in glucose metabolism (as measured by changes in FDG uptake) with changes in AKT pathway biomarkers, as an alternative to invasive tumour biopsies. The third aim of this study was to assess the role of DNA-PK mediated activation of AKT, compared to PI3K, in mediating drug resistance. The results demonstrate that GSK2141795 synergistically enhances cisplatin induced apoptosis in platinum-resistant ovarian cancer cells, grown as either 2-dimensional monolayers or 3-dimensional multi-cellular tumour spheroids (MTS). Similar results were achieved when cisplatin was combined with the DNA-PK inhibitor NU7441, but not in combination with the selective PI3K inhibitor GSK2126458 in vitro. In vivo, combination of GSK2141795 or NU7441 with cisplatin led to superior tumour growth inhibition in murine xenografts, compared to either agent alone. In all three model systems (2D monolayers 3D MTS and in vivo xenografts), GSK2141795 decreased levels of phospho-PRAS40 and FDG uptake. FDG uptake and pPRAS40 expression were strongly correlated. In addition, reverse phase protein arrays (RPPA) was used to study the effects of GSK2141795 on cell signalling pathways in vitro, in vivo and using tumour biopsies from a phase I clinical trial of GSK2141795 in ovarian cancer. This identified a signature of AKT-pathway inhibition that includes consistent changes in phosphorylation of S6, 4E-BP1 and AKT itself. Taken together, this report shows that GSK2141795 inhibits AKT signalling in platinum-resistant ovarian cancer cells, and can reverse platinum resistance in pre-clinical systems, and merits clinical exploration. In addition, the results demonstrate significant differences in the role of DNA-PK and PI3K mediated AKT activation in modulating the response to platinum chemotherapy, and support further clinical development of AKT or DNA-PK inhibitors in combination with cisplatin to overcome platinum resistance in ovarian cancer. FDG uptake was also found to be potential non-invasive PD biomarker, subject to clinical validation, for guiding dose selection in ovarian cancer patients. RPPA also identified a proteomic PD signature that could be used for pre-clinical development and applied to clinical tumour biopsies.

Thesis (Ph. D.)--Ohio State University, 2003.
Title from first page of PDF file. Document formatted into pages; contains xv, 219 p.; also includes graphics (some col.) Includes bibliographical references (p. 147-173). Available online via OhioLINK's ETD Center

The thesis offers a critical analysis of the transmission of Seneca's Thyestes in the sixteenth and seventeenth centuries. In Volume 1, the 1584 Gryphius edition of Seneca's Thyestes; the 1560 edition of Heywood's translation of the play and the 1674 edition of Wright's translation and burlesque version have been transcribed. This is the first time that these texts have been presented together for discussion. The commentary (Volume II) examines a broad range of dramatic material including Neo- Latin plays such as Goldingham's Herodes (1570/80); Gwinne's Nero (1603); Snelling's Thibaldus (1640) and the anonymous Stoicus Vapulans (1648). Prose works considered include the Latin lexicas and grammars of Lilly and Whitinton; philosophical treatises such as Reynolds A Treatise of the Passions and Faculties of the soule of Man (1640); and religious works such as Hooper on the Ten Commandments (1560). It presents hitherto unpublished material- MS Sloane 1041; and material that has previously received little attention- the Hendrik Goltzius' engraving of Melpomene (1592) and the Restoration Mock-Thyestes in Burlesque. Research material was consulted at the British Library; BL Department of Manuscripts; BL Print Room; University of Warwick Library; University of Birmingham Library; Senate House Library, University of London; The Bodleian Library, University of Oxford; The Warburg Institute and The Institute of Classical Studies.

Image registration, the process of finding the transformation that best maps one image to another, is an important tool in document image processing. Having properly-aligned microfilm images can help in manual and automated content extraction, zoning, and batch compression of images. An image registration algorithm is presented that quickly identifies the global affine transformation (rotation, scale, translation and/or shear) that maps one tabular document image to another, using the Fourier-Mellin Transform. Each component of the affine transform is recovered independantly from the others, dramatically reducing the parameter space of the problem, and improving upon standard Fourier-Mellin Image Registration (FMIR), which only directly separates translation from the other components. FMIR is also extended to handle shear, as well as different scale factors for each document axis. This registration method deals with all transform components in a uniform way, by working in the frequency domain. Registration is limited to foreground pixels (the document form and printed text) through the introduction of a novel, locally adaptive foreground-background segmentation algorithm, based on the median filter. The background removal algorithm is also demonstrated as a useful tool to remove ambient signal noise during correlation. Common problems with FMIR are eliminated by background removal, meaning that apodization (tapering down to zero at the edge of the image) is not needed for accurate recovery of the rotation parameter, allowing the entire image to be used for registration. An effective new optimization to the median filter is presented. Rotation and scale parameter detection is less susceptible to problems arising from the non-commutativity of rotation and "tiling" (periodicity) than for standard FMIR, because only the regions of the frequency domain directly corresponding to tabular features are used in registration. An original method is also presented for automatically obtaining blank document templates from a set of registered document images, by computing the "pointwise median" of a set of registered documents. Finally, registration is demonstrated as an effective tool for predictive image compression. The presented registration algorithm is reliable and robust, and handles a wider range of transformation types than most document image registration systems (which typically only perform deskewing).

SerpinA12 (vaspin) is thought to be mainly expressed in adipose tissue and has multiple beneficial effects on metabolic, inflammatory and atherogenic processes related to obesity. KLK7 (kallikrein 7) is the only known protease target of vaspin to date and is inhibited with a moderate inhibition rate. In the crystal structure, the cleavage site (P1-P1′) of the vaspin reactive centre loop is fairly rigid compared with the flexible residues before P2, possibly supported by an ionic interaction of P1′ glutamate (Glu379) with an arginine residue (Arg302) of the β-sheet C. A P1′ glutamate seems highly unusual and unfavourable for the protease KLK7. We characterized vaspin mutants to investigate the roles of these two residues in protease inhibition and recognition by vaspin. Reactive centre loop mutations changing the P1′ residue or altering the reactive centre loop conformation significantly increased inhibition parameters, whereas removal of the positive charge within β-sheet C impeded the serpin–protease interaction. Arg302 is a crucial contact to enable vaspin recognition by KLK7 and it supports moderate inhibition of the serpin despite the presence of the detrimental P1′ Glu379, which clearly represents a major limiting factor for vaspin-inhibitory activity. We also show that the vaspin-inhibition rate for KLK7 can be modestly increased by heparin and demonstrate that vaspin is a heparin-binding serpin. Noteworthily, we observed vaspin as a remarkably thermostable serpin and found that Glu379 and Arg302 influence heat-induced polymerization. These structural and functional results reveal the mechanistic basis of how reactive centre loop sequence and exosite interaction in vaspin enable KLK7 recognition and regulate protease inhibition as well as stability of this adipose tissue-derived serpin.

Today, the main research field for the automotive industry is to find solutions for active safety. In order to perceive the surrounding environment, tracking nearby traffic objects plays an important role. Validation of the tracking performance is often done in staged traffic scenarios, where additional sensors, mounted on the vehicles, are used to obtain their true positions and velocities. The difficulty of evaluating the tracking performance complicates its development. An alternative approach studied in this thesis, is to record sequences and use non-causal algorithms, such as smoothing, instead of filtering to estimate the true target states. With this method, validation data for online, causal, target tracking algorithms can be obtained for all traffic scenarios without the need of extra sensors. We investigate how non-causal algorithms affects the target tracking performance using multiple sensors and dynamic models of different complexity. This is done to evaluate real-time methods against estimates obtained from non-causal filtering. Two different measurement units, a monocular camera and a LIDAR sensor, and two dynamic models are evaluated and compared using both causal and non-causal methods. The system is tested in two single object scenarios where ground truth is available and in three multi object scenarios without ground truth. Results from the two single object scenarios shows that tracking using only a monocular camera performs poorly since it is unable to measure the distance to objects. Here, a complementary LIDAR sensor improves the tracking performance significantly. The dynamic models are shown to have a small impact on the tracking performance, while the non-causal application gives a distinct improvement when tracking objects at large distances. Since the sequence can be reversed, the non-causal estimates are propagated from more certain states when the target is closer to the ego vehicle. For multiple object tracking, we find that correct associations between measurements and tracks are crucial for improving the tracking performance with non-causal algorithms.

Il lavoro è stato suddiviso in tre macro-aree. Una prima riguardante un'analisi teorica di come funzionano le intrusioni, di quali software vengono utilizzati per compierle, e di come proteggersi (usando i dispositivi che in termine generico si possono riconoscere come i firewall). Una seconda macro-area che analizza un'intrusione avvenuta dall'esterno verso dei server sensibili di una rete LAN. Questa analisi viene condotta sui file catturati dalle due interfacce di rete configurate in modalità promiscua su una sonda presente nella LAN. Le interfacce sono due per potersi interfacciare a due segmenti di LAN aventi due maschere di sotto-rete differenti. L'attacco viene analizzato mediante vari software. Si può infatti definire una terza parte del lavoro, la parte dove vengono analizzati i file catturati dalle due interfacce con i software che prima si occupano di analizzare i dati di contenuto completo, come Wireshark, poi dei software che si occupano di analizzare i dati di sessione che sono stati trattati con Argus, e infine i dati di tipo statistico che sono stati trattati con Ntop. Il penultimo capitolo, quello prima delle conclusioni, invece tratta l'installazione di Nagios, e la sua configurazione per il monitoraggio attraverso plugin dello spazio di disco rimanente su una macchina agent remota, e sui servizi MySql e DNS. Ovviamente Nagios può essere configurato per monitorare ogni tipo di servizio offerto sulla rete.

Bien qu’ils soient largement utilisés dans le domaine, les mesures vibratoires classiques présentent plusieurs limites. A la base, l’analyse vibratoire ne peut identifier qu’environ 60% des défauts qui peuvent survenir dans les machines. Cependant, les principaux inconvénients des mesures de la vibration sont l’accès difficile au système de transmission afin d’y placer le capteur ainsi que le coût conséquent de la mise en œuvre. Ceci résulte en des problèmes de sensibilité relatifs à la position de l’installation et ceux de difficulté pour distinguer la source de vibration à cause de la diversité des excitations mécaniques qui existent dans l’environnement industriel.Par conséquent, l’analyse des signatures du courant électrique des moteurs s’impose comme une alternative prometteuse à l’analyse vibratoire et a donc fait l’objet d’une attention grandissante au cours des dernières années. En effet, l’analyse des signatures électriques a l’avantage d’être une méthode techniquement accessible, non-intrusive au système et peu coûteuse. Les techniques basées sur le courant et la tension ne requièrent que les mesures électriques du moteur qui sont souvent déjà surveillées pour le contrôle et la protection des machines électriques. Ce processus a été principalement utilisé pour la détection des défauts de moteur tels que la rupture de barres du rotor et les défauts d’excentricité ainsi que les défauts de roulements. En revanche, très peu de recherches concernent la détection des défauts en utilisant l’analyse du courant. En outre, les signaux électriques triphasés sont caractérisés par des représentations géométriques particulières liées à leur forme d’onde qui peuvent servir en tant qu’indicateurs différents offrant des informations supplémentaires. Parmi ces indicateurs géométriques, les transformées de Park et de Concordia modélisent les composantes électriques dans un repère bidimensionnel et toute déviation par rapport à la représentation d’origine indique l’apparition d’un dysfonctionnement. Aussi, les équations différentielles de Frenet-Serret représentent la trajectoire du signal dans un espace euclidien tridimensionnel et indiquent ainsi tout changement dans l’état du système. Bien qu’ils aient été utilisés pour les défauts de roulements, ces indicateurs n’ont pas été appliqués dans la détection des défauts d’engrenages en utilisant l’analyse des signatures des courants électriques. D’où l’idée novatrice de combiner ces indicateurs avec des techniques de traitement de signal, ainsi que des techniques de classification pour le diagnostic des engrenages en utilisant l’analyse des signatures de courant et de tension du moteur électrique.Ainsi, dans ce travail, on propose une nouvelle approche pour le diagnostic des défauts d’engrenages en utilisant l’analyse des courants et des tensions électriques du stator de la machine et ceci en se basant sur un ensemble d’indicateurs géométriques (Transformées de Park et de Concordia ainsi que les propriétés du repère Frenet-Serret). Ces indicateurs font partie d’une bibliothèque de signatures de défauts qui a été construite et qui comprend également les indicateurs classiques utilisés pour un large éventail de défauts. Ainsi, un algorithme combine les acquisitions expérimentales des signaux électriques à des méthodes de traitement de signal avancées (décomposition modale empirique,…). Ensuite, celui-ci sélectionne les indicateurs les plus pertinents au sein de la bibliothèque en se basant sur les algorithmes de sélection de paramètres (sélection séquentielle rétrograde et analyse des composantes principales). Enfin, cette sélection est utilisée pour la classification non-supervisée (K-moyennes) pour la distinction entre l’état sain et l’état défaillant
Although they are widely used, classical vibration measurements have several limitations. Vibration analysis can only identify about 60% of the defects that may occur in mechanical systems. However, the main drawbacks of vibration measurements are the difficult access to the transmission system in order to place the sensor as well as the consequent cost of implementation. This results in sensitivity problems relative to the position of the installation and the difficulty to distinguish the source of vibration because of the diversity of mechanical excitations that exist in the industrial environment.Hence, the Motor Current Signatures Analysis (M.C.S.A.) represents a promising alternative to the vibration analysis and has therefore been the subject of increasing attention in recent years. Indeed, the analysis of electrical signatures has the advantage of being a technically accessible method as well as inexpensive and non-intrusive to the system. Techniques based on currents and voltages only require the motor’s electrical measurements which are often already supervised for the purposes of the control and the protection of the electrical machines. This process was mainly used for the detection of motors faults such as rotor bars breakage and eccentricity faults as well as bearings defects. On the other hand, very little research has been focused on gear faults detection using the current analysis. In addition, three-phase electrical signals are characterized by specific geometric representations related to their waveforms and they can serve as different indicators providing additional information. Among these geometric indicators, the Park and Concordia transforms model the electrical components in a two-dimensional coordinate system and any deviation from the original representation indicates the apparition of a malfunction. Moreover, the differential equations of Frenet-Serret represent the trajectory of the signal in a three-dimensional euclidean space and thus indicate any changes in the state of the system. Although they have been previously used for bearing defects, these indicators have not been applied in the detection of gear defects using the analysis of electrical current signatures. Hence, the innovative idea of combining these indicators with signal processing techniques, as well as classification techniques for gears diagnosis using the three-phase motor’s electrical current signatures analysis is established.Hence, in this work, a new approach is proposed for gear faults diagnosis using the motor currents analysis, based on a set of geometric indicators (Park and Concordia transforms as well as the properties of the Frenet-Serret frame). These indicators are part of a specifically built fault signatures library and which also includes the classical indicators used for a wide range of faults. Thus, a proposed estimation algorithm combines experimental measurements of electrical signals with advanced signal processing methods (Empirical Mode Decomposition, ...). Next, it selects the most relevant indicators within the library based on feature selection algorithms (Sequential Backward Selection and Principal Component Analysis). Finally, this selection is combined with non-supervised classification (K-means) for the distinction between the healthy state and faulty states. It was finally validated with a an additional experimental configuration in different cases with gear faults, bearing faults and combined faults with various load levels

Reverse genetics is an innovative molecular biology tool that enables the manipulation of viral genomes at the cDNA level in order to generate particular mutants or artificial viruses. The reverse genetics system for the influenza virus is arguably one of the best illustrations of the potential power of this technology. This reverse genetics system is the basis for the ability to regularly adapt influenza vaccines strains. Today, reverse genetic systems have been developed for many animal RNA viruses. Selection-free reverse genetics systems have been developed for the members of the Reoviridae family including, African horsesickness virus, bluetongue virus and orthoreovirus. This ground-breaking technology has led to the generation of valuable evidence regarding the replication and pathogenesis of these viruses. Unfortunately, extrapolating either the plasmid-based or transcript-based reverse genetics systems to rotavirus has not yet been successful. The development of a selection-free rotavirus reverse genetics system will enable the systematic investigation of poorly understood aspects of the rotavirus replication cycle and aid the development of more effective vaccines, amongst other research avenues. This study investigated the importance of co-expressed rotavirus proteins in the development of a selection-free rotavirus reverse genetics system. The consensus sequences of the rotavirus strains Wa (RVA/Human-tc/USA/WaCS/1974/G1P[8]) and SA11 (RVA/Simian-tc/ZAF/SA11/1958/G3P[2]) where used to design rotavirus expression plasmids. The consensus nucleotide sequence of a human rotavirus Wa strain was determined by sequence-independent cDNA synthesis and amplification combined with next-generation 454® pyrosequencing. A total of 4 novel nucleotide changes, which also resulted in amino acid changes, were detected in genome segment 7 (NSP3), genome segment 9 (VP7) and genome segment 10 (NSP4). In silico analysis indicated that none of the detected nucleotide changes, and consequent amino acid variations, had any significant effect on viral structure. Evolutionary analysis indicated that the sequenced rotavirus WaCS was closely related to the ParWa and VirWa variants, which were derived from the original 1974 Wa isolate. Despite serial passaging in animals, as well as cell cultures, the Wa genome seems to be stable. Considering that the current reference sequence for the Wa strain is a composite sequence of various Wa variants, the rotavirus WaCS may be a more appropriate reference sequence. The rotavirus Wa and SA11 strains were selected for plasmid-based expression of rotavirus proteins, under control of a T7 promoter sequence, due to the fact that they propagate well in MA104 cells and the availability of their consensus sequences. The T7 RNA polymerase was provided by a recombinant fowlpox virus. After extensive transfection optimisation on a variety of mammalian cell lines, MA104 cells proved to be the best suited for the expression rotavirus proteins from plasmids. The expression of rotavirus Wa and SA11 VP1, VP6, NSP2 and NSP5 could be confirmed with immunostaining in MA104 and HEK 293H cells. Another approach involved the codon-optimised expression of the rotavirus replication complex scaffold in MA104 cells under the control of a CMV promoter sequence. This system was independent from the recombinant fowlpox virus. All three plasmid expression sets were designed to be used in combination with the transcript-based reverse genetics system in order to improve the odds of developing a successful rotavirus reverse genetics system. Rotavirus transcripts were generated using transcriptively active rotavirus SA11 double layered particles (DLPs). MA104 and HEK293H cells proved to be the best suited for the expression of rotavirus transcripts although expression of rotavirus VP6 could be demonstrated in all cell cultures examined (MA104, HEK 293H, BSR and COS-7) using immunostaining. In addition, the expression of transcript derived rotavirus VP1, NSP2 and NSP5 could be confirmed with immunofluorescence in MA104 and HEK 293H cells. This is the first report of rotavirus transcripts being translated in cultured cells. A peculiar cell death pattern was observed within 24 hours in response to transfection of rotavirus transcripts. This observed cell death, however does not seem to be related to normal viral cytopathic effect as no viable rotavirus could be recovered. In an effort to combine the transcript- and plasmid systems, a dual transfection strategy was followed where plasmids encoding rotavirus proteins were transfected first followed, 12 hours later, by the transfection of rotavirus SA11 transcripts. The codon- optimised plasmid system was designed as it was postulated that expression of the DLP-complex (VP1, VP2, VP3 and VP6), the rotavirus replication complex would form and assist with replication and/or packaging. Transfecting codon- optimized plasmids first noticeably delayed the mass cell death observed when transfecting rotavirus transcripts on their own. None of the examined coexpression systems were able to produce a viable rotavirus. Finally, the innate immune responses elicited by rotavirus transcripts and plasmid-derived rotavirus Wa and SA11 proteins were investigated. Quantitative RT-PCR (qRT-PCR) experiments indicated that rotavirus transcripts induced high levels of the expression of the cytokines IFN- α1, IFN-1β, IFN-λ1 and CXCL10. The expression of certain viral proteins from plasmids (VP3, VP7 and NSP5/6) was more likely to stimulate specific interferon responses, while other viral proteins (VP1, VP2, VP4 and NSP1) seem to be able to actively suppress the expression of certain cytokines. In the light of these suppression results, specific rotavirus proteins were expressed from transfected plasmids to investigate their potential in supressing the interferon responses provoked by rotavirus transcripts. qRT-PCR results indicated that cells transfected with the plasmids encoding NSP1, NSP2 or a combination of NSP2 and NSP5 significantly reduced the expression of specific cytokines induced by rotavirus transcripts. These findings point to other possible viral innate suppression mechanisms in addition to the degradation of interferon regulatory factors by NSP1. The suppression of the strong innate immune response elicited by rotavirus transcripts might well prove to be vital in the quest to better understand the replication cycle of this virus and eventually lead to the development of a selection-free reverse genetics system for rotavirus.
PhD (Biochemistry), North-West University, Potchefstroom Campus, 2014

O presente trabalho de projeto tem como objetivo a tradução e revisão do índice e de cinco verbetes respeitantes à enciclopédia de Estudos de Tradução, Handbook of Translation Studies (2010-2016), editada por Yves Gambier e Luc Van Doorslaer. A obra em análise destina-se a estudantes, profissionais e especialistas, servindo de apoio para solucionar problemas de tradução, interpretação ou localização. O presente trabalho pretende sensibilizar para (1) a importância dos Estudos de Tradução; (2) as dificuldades do processo tradutório e de revisão de artigos académicos; (3) o papel da investigação na profissão de tradutor. Para tal, realiza-se uma análise global, de forma a expor as principais problemáticas e soluções encontradas. Esta análise pretende ainda realçar a importância da consulta de material de referência, como dicionários, gramáticas e glossários. Além da tradução dos verbetes, é ainda analisada a tradução do índice da obra, sugerida pelos editores, que tem o objetivo estabelecer os títulos de todos os artigos da enciclopédia em português, ainda que a maioria destes textos não tenha sido traduzida. Além do contacto com autores e editores relevantes, este projeto permitirá a publicação online dos artigos aqui trabalhados. Considero ainda que este projeto impulsionará os Estudos de Tradução, onde ainda se verifica pouco material de pesquisa e investigação em português, através do estabelecimento de terminologia nas temáticas abordadas.
The scope of this project involves translating and editing the index and five articles related to the encyclopedia Handbook of Translation Studies (2010-2016), edited by Yves Gambier and Luc Van Doorslaer. The encyclopedia addresses students, professionals and specialists by serving as a support to solve translation, interpretation or localization problems. This projects aims to sensitize to the importance of Translation Studies; the difficulties of translating and editing articles; and the role of research in translation. An overall analysis is also carried out, in order to expose the main difficulties and solutions encountered during the translation and revision process. This analysis also emphasizes the importance of using dictionaries, grammars and glossaries as reference material. In addition to the translation of the articles, it is also analyzed the translation of the index: a suggestion made by the editors that aims to establish the titles of all the articles of the encyclopedia in Portuguese, although most of these texts have not been translated. This project has enabled the contact with relevant authors and editors, and will lead to the online publication of the articles. It is also intended to enhance the field of Translation Studies, where there is still little research material in Portuguese, through the establishment of terminology in the areas approached.