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Journal articles on the topic 'RFBP'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

McClelland, D. A., S. H. McLaughlin, R. B. Freedman, and N. C. Price. "The refolding of hen egg white riboflavin-binding protein: effect of protein disulphide isomerase on the reoxidation of the reduced protein." Biochemical Journal 311, no. 1 (1995): 133–37. http://dx.doi.org/10.1042/bj3110133.

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Hen egg white riboflavin-binding protein (RfBP) contains nine disulphide bonds. Provided these remain intact, the refolding of RfBP after incubation in 6 M guanidinium chloride is highly efficient with at least 95% of the binding activity regained within 3 min. Kinetic studies indicate that this regain consists of at least two phases. When the disulphide bonds of RfBP are reduced, reoxidation using a mixture of oxidized and reduced glutathione leads to less than 5% recovery of activity. However, if protein disulphide isomerase (PDI; EC 5.3.4.1) is present during the reoxidation nearly 50% acti

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P., Koteshwar Rao* G. Rajender. "SCREENING OF RIBOFLAVIN BINDING PROTEIN (RFBP) FROM AVIAN EGGS TO SCREEN THEIR ANTI-CANCER ACTIVITY." INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH 07, no. 01 (2017): 7323–28. https://doi.org/10.5281/zenodo.1005162.

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Riboflavin binding protein (Rfbp) was purified from different avian eggs including Pea cock (Pavo cristatus) Egg- white and Hen (Gallus gallus) egg- white Riboflavin binding protein (RfBP) was isolated. The Rfbp was purified in two steps; DEAE-SephadexA-50 ion exchange chromatography. The final purification of proteins (Rfbp) was achieved on SephadexG100.The protein content was estimated with Lowry method. The purity of the proteins was judged by two methods like cylindrical and slab-gels, SDS- PAGE techniques. These proteins showed a single band on SDS gels and the molecular weight was 29 Kil

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Monaco, Hugo L. "Coordinates of the molecule of Chicken Eggwhite Riboflavin-binding Protein." EMBO Journal 16, no. 7 (2022): 1475–83. https://doi.org/10.5281/zenodo.7090285.

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Sánchez-Gorostiaga, Alicia, Carlos López-Estraño, Dora B. Krimer, Jorge B. Schvartzman, and Pablo Hernández. "Transcription Termination Factor reb1p Causes Two Replication Fork Barriers at Its Cognate Sites in Fission Yeast Ribosomal DNA In Vivo." Molecular and Cellular Biology 24, no. 1 (2004): 398–406. http://dx.doi.org/10.1128/mcb.24.1.398-406.2004.

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ABSTRACT Polar replication fork barriers (RFBs) near the 3′ end of the rRNA transcriptional unit are a conserved feature of ribosomal DNA (rDNA) replication in eukaryotes. In the mouse, in vivo studies indicate that the cis-acting Sal boxes required for rRNA transcription termination are also involved in replication fork blockage. On the contrary, in the budding yeast Saccharomyces cerevisiae, the rRNA transcription termination factors are not required for RFBs. Here we characterized the rDNA RFBs in the fission yeast Schizosaccharomyces pombe. S. pombe rDNA contains three closely spaced polar

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Hidalgo-Cantabrana, C., I. Ordoñez, P. Ruas-Madiedo, and A. Margolles. "Degenerate PCR primers for detecting putative priming glycosyltransferase genes in Bifidobacterium strains." Beneficial Microbes 6, no. 4 (2015): 553–62. http://dx.doi.org/10.3920/bm2014.0046.

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A new PCR-based method to detect putative exopolysaccharide (EPS) producers from the genus Bifidobacterium was developed based on the detection of two priming glycosyltransferase genes: rfbP (undecaprenyl-phosphate sugar phospho-transferase) and cpsD (galactosyl-transferase). An in silico analysis of the genomes of 28 bifidobacterial strains, belonging to 8 different species, allowed us to detect rfbP, cpsD, or both, in the large majority of the genomes. Based on DNA sequence homology studies, 24 degenerated primers were synthesised in order to select the primer pairs with the broadest capacit

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Curti, Nico, Daniele Dall’Olio, Daniel Remondini, Gastone Castellani, and Enrico Giampieri. "rFBP: Replicated Focusing Belief Propagation algorithm." Journal of Open Source Software 5, no. 54 (2020): 2663. http://dx.doi.org/10.21105/joss.02663.

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Liu, Zongmin, Lingzhi Li, Qianwen Wang, et al. "Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation." Microorganisms 9, no. 2 (2021): 385. http://dx.doi.org/10.3390/microorganisms9020385.

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Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of Bifidobacterium longum FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein–protein interaction network analysis of 1296 differentially expressed genes during biofilm formation yielded 15 clusters of highly interconnected nodes, indicating that genes related to th

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8

Kumari, Reena, Ashok Kumar Pal, Elbaz I. Abouelmagd, and Sawsan Alhowaity. "Approximation Solution of the Nonlinear Circular Sitnikov Restricted Four–Body Problem." Symmetry 13, no. 10 (2021): 1966. http://dx.doi.org/10.3390/sym13101966.

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In this paper, the approximated periodic solutions of the circular Sitnikov restricted four–body problem (RFBP) were constructed using the Lindstedt–Poincaré method, by removing the secular terms, and compared with numerical solution. It can be observed that, in the numerical as well as approximated solutions patterns, the initial conditions are important. In the sense of a numerical solution, the motion is periodic in a certain interval, but beyond this interval, the motion is not periodic. But, the Lindstedt–Poincaré method constantly gives regular and periodic motion all time. Finally, we o

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9

Uchenna Nwanodi Nwankwo and Obioma Kelechi Agwa. "Identification of exopolysaccharide synthesizing genes in bacteria isolated from Bonny River in Rivers State, Nigeria." International Journal of Science and Research Archive 11, no. 1 (2024): 2461–68. http://dx.doi.org/10.30574/ijsra.2024.11.1.0752.

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Microbial polysaccharide is the most important biopolymer secreted either by bacteria, fungi, or yeast as natural non-toxic, biodegradable and renewable sugar monomers. Microbial EPS owing to their interesting physicochemical and rheological properties has a wide range of industrial application such as in pharmaceuticals, cosmetics, food and Microbial enhanced oil recovery. This study was aimed to identify exopolysaccharide synthesizing genes in three bacterial isolates from Bonny River in Rivers State, Nigeria. The bacterial isolates were identified using the 16S rRNA gene sequence of the str

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10

Shornikov, Aleksander, Ha Tran, Jennifer Macias, et al. "Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose 3,5-epimerase (RfbC)." Acta Crystallographica Section F Structural Biology Communications 73, no. 12 (2017): 664–71. http://dx.doi.org/10.1107/s2053230x17015849.

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The exosporium layer of Bacillus anthracis spores is rich in L-rhamnose, a common bacterial cell-wall component, which often contributes to the virulence of pathogens by increasing their adherence and immune evasion. The biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes (RfbA, RfbB, RfbC and RfbD) and is an attractive drug target because there are no homologs in mammals. It was found that co-purifying and screening RfbC (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) from B. anthracis in the presence of the other three B. anthracis enzymes

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11

Chen, Yu-Ju, Tsung-Chuan Huang, and Rey-Chue Hwang. "An effective learning of neural network by using RFBP learning algorithm." Information Sciences 167, no. 1-4 (2004): 77–86. http://dx.doi.org/10.1016/j.ins.2003.10.012.

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12

Hewetson, Aveline, Amber E. Wright-Pastusek, Rebecca A. Helmer, Kerrie A. Wesley, and Beverly S. Chilton. "Conservation of inter-protein binding sites in RUSH and RFBP, an ATP11B isoform." Molecular and Cellular Endocrinology 292, no. 1-2 (2008): 79–86. http://dx.doi.org/10.1016/j.mce.2008.05.007.

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13

Alvarez-Ramírez, Martha, Joaquín Delgado, and Claudio Vidal. "Global Regularization of a Restricted Four-Body Problem." International Journal of Bifurcation and Chaos 24, no. 07 (2014): 1450092. http://dx.doi.org/10.1142/s0218127414500928.

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In the n-body problem, a collision singularity occurs when the position of two or more bodies coincide. By understanding the dynamics of collision motion in the regularized setting, a better understanding of the dynamics of near-collision motion is achieved. In this paper, we show that any double collision of the planar equilateral restricted four-body problem can be regularized by using a Birkhoff-type transformation. This transformation has the important property to provide a simultaneous regularization of three singularities due to binary collision. We present some ejection–collision orbits

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14

Schäffer, Christina, Thomas Wugeditsch, Paul Messner, and Chris Whitfield. "Functional Expression of Enterobacterial O-Polysaccharide Biosynthesis Enzymes in Bacillus subtilis." Applied and Environmental Microbiology 68, no. 10 (2002): 4722–30. http://dx.doi.org/10.1128/aem.68.10.4722-4730.2002.

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ABSTRACT The expression of heterologous bacterial glycosyltransferases is of interest for potential application in the emerging field of carbohydrate engineering in gram-positive organisms. To assess the feasibility of using enzymes from gram-negative bacteria, the functional expression of the genes wbaP (formerly rfbP), wecA (formerly rfe), and wbbO (formerly rfbF) from enterobacterial lipopolysaccharide O-polysaccharide biosynthesis pathways was examined in Bacillus subtilis. WbaP and WecA are initiation enzymes for O-polysaccharide formation, catalyzing the transfer of galactosyl 1-phosphat

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15

Mejía-Ramírez, Eva, Alicia Sánchez-Gorostiaga, Dora B. Krimer, Jorge B. Schvartzman, and Pablo Hernández. "The Mating Type Switch-Activating Protein Sap1 Is Required for Replication Fork Arrest at the rRNA Genes of Fission Yeast." Molecular and Cellular Biology 25, no. 19 (2005): 8755–61. http://dx.doi.org/10.1128/mcb.25.19.8755-8761.2005.

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ABSTRACT Schizosaccharomyces pombe rRNA genes contain three replication fork barriers (RFB1-3) located in the nontranscribed spacer. RFB2 and RFB3 require binding of the transcription terminator factor Reb1p to two identical recognition sequences that colocalize with these barriers. RFB1, which is the strongest of the three barriers, functions in a Reb1p-independent manner, and cognate DNA-binding proteins for this barrier have not been identified yet. Here we functionally define RFB1 within a 78-bp sequence located near the 3′ end of the rRNA coding region. A protein that specifically binds t

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16

Li, Yin, Liang Huang, Chang Gao, et al. "Axial compressive behavior of steel reinforced GGBS-RFBP-FA ternary composite geopolymer recycled fireclay brick aggregates concrete columns." Structures 60 (February 2024): 105913. http://dx.doi.org/10.1016/j.istruc.2024.105913.

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17

Wang, L., and P. R. Reeves. "Involvement of the galactosyl-1-phosphate transferase encoded by the Salmonella enterica rfbP gene in O-antigen subunit processing." Journal of Bacteriology 176, no. 14 (1994): 4348–56. http://dx.doi.org/10.1128/jb.176.14.4348-4356.1994.

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18

Li, Yin, Liang Huang, Chang Gao, Zhijie Mao, and Mingzhu Qin. "Workability and mechanical properties of GGBS-RFBP-FA ternary composite geopolymer concrete with recycled aggregates containing recycled fireclay brick aggregates." Construction and Building Materials 392 (August 2023): 131450. http://dx.doi.org/10.1016/j.conbuildmat.2023.131450.

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19

Rajender, G. "A docile method of isolation and purification of riboflavin binding protein (Rfbp) from peahen (Pavo cristatus) and domestic fowl (Gallus gallus)." Indian Journal of Science and Technology 2, no. 11 (2009): 32–34. http://dx.doi.org/10.17485/ijst/2009/v2i11.8.

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20

Pławińska-Czarnak, Joanna, Karolina Wódz, Magdalena Guzowska, et al. "Comparison of Phenotype and Genotype Virulence and Antimicrobial Factors of Salmonella Typhimurium Isolated from Human Milk." International Journal of Molecular Sciences 24, no. 6 (2023): 5135. http://dx.doi.org/10.3390/ijms24065135.

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Salmonella is a common foodborne infection. Many serovars belonging to Salmonella enterica subsp. enterica are present in the gut of various animal species. They can cause infection in human infants via breast milk or cross-contamination with powdered milk. In the present study, Salmonella BO was isolated from human milk in accordance with ISO 6579-1:2017 standards and sequenced using whole-genome sequencing (WGS), followed by serosequencing and genotyping. The results also allowed its pathogenicity to be predicted. The WGS results were compared with the bacterial phenotype. The isolated strai

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21

Wang, L., D. Liu, and P. R. Reeves. "C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesis." Journal of bacteriology 178, no. 9 (1996): 2598–604. http://dx.doi.org/10.1128/jb.178.9.2598-2604.1996.

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22

Kong, Qingke, Jiseon Yang, Qing Liu, Praveen Alamuri, Kenneth L. Roland, and Roy Curtiss. "Effect of Deletion of Genes Involved in Lipopolysaccharide Core and O-Antigen Synthesis on Virulence and Immunogenicity of Salmonella enterica Serovar Typhimurium." Infection and Immunity 79, no. 10 (2011): 4227–39. http://dx.doi.org/10.1128/iai.05398-11.

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ABSTRACTLipopolysaccharide (LPS) is a major virulence factor ofSalmonella entericaserovar Typhimurium and is composed of lipid A, core oligosaccharide (C-OS), and O-antigen polysaccharide (O-PS). While the functions of the gene products involved in synthesis of core and O-antigen have been elucidated, the effect of removing O-antigen and core sugars on the virulence and immunogenicity ofSalmonella entericaserovar Typhimurium has not been systematically studied. We introduced nonpolar, defined deletion mutations inwaaG(rfaG),waaI(rfaI),rfaH,waaJ(rfaJ),wbaP(rfbP),waaL(rfaL), orwzy(rfc) into wild

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23

Campana, D., G. Janossy, M. Bofill, et al. "Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue." Journal of Immunology 134, no. 3 (1985): 1524–30. http://dx.doi.org/10.4049/jimmunol.134.3.1524.

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Abstract The phenotype of B lineage cells (TdT+, pre-B, IgM+, IgD-, and IgM+,IgD+) in infant and adult human bone marrow was compared with that of B cells seen in peripheral tissues such as tonsil and blood. The range of B cell-associated antibodies used included four reagents with greater than 90% reactivity on peripheral B cells: RFB4 and To15 (both p135, corresponding to CD22), RFB6 (p140 corresponding to CD21), and Y29/55, a unique B cell-specific antibody. In addition, AL-1, an antibody with virtually no reactivity against peripheral B cells was also used. The BM cell subpopulations were

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Bofill, M., G. Janossy, M. Janossa, et al. "Human B cell development. II. Subpopulations in the human fetus." Journal of Immunology 134, no. 3 (1985): 1531–38. http://dx.doi.org/10.4049/jimmunol.134.3.1531.

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Abstract In man, during fetal development the B cell populations show distinct phenotypes at different tissue sites. The pre-B and B lymphocytes of the fetal liver and bone marrow express IgM and B cell markers, B1 (CD20) and BA-1 (CD24). These "early" cells are negative with a number of other reagents, anti-IgD, RFB4 (CD22), RFB6 (CD21), and RFA-2, which on the other hand recognize peripheral B cells. These peripheral B lymphocytes in the developing fetus are heterogeneous. The diffusely distributed B cells in the earliest lymph node samples, 16 to 17 wk of gestational age, and from 16 to 21

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Wang, Ying, Pan Chen, Qi Lin, Linzhi Zuo, and Lei Li. "Whole-Genome Sequencing of Two Potentially Allelopathic Strains of Bacillus from the Roots of C. equisetifolia and Identification of Genes Related to Synthesis of Secondary Metabolites." Microorganisms 12, no. 6 (2024): 1247. http://dx.doi.org/10.3390/microorganisms12061247.

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The coastal Casuarina equisetifolia is the most common tree species in Hainan’s coastal protection forests. Sequencing the genomes of its allelopathic endophytes can allow the protective effects of these bacteria to be effectively implemented in protected forests. The goal of this study was to sequence the whole genomes of the endophytes Bacillus amyloliquefaciens and Bacillus aryabhattai isolated from C. equisetifolia root tissues. The results showed that the genome sizes of B. amyloliquefaciens and B. aryabhattai were 3.854 Mb and 5.508 Mb, respectively. The two strains shared 2514 common ge

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26

Coimbra, Roney S., Francine Grimont, Pascal Lenormand, Pierre Burguière, Lothar Beutin, and Patrick A. D. Grimont. "Identification of Escherichia coli O-serogroups by restrictionof the amplified O-antigen gene cluster (rfb-RFLP)." Research in Microbiology 151, no. 8 (2000): 639–54. http://dx.doi.org/10.1016/s0923-2508(00)00134-0.

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27

Juul-Madsen, H. R., Rima Zoorob, Charles Auffray, Karsten Skjødt, and Jan E. Hedemand. "New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses." Immunogenetics 45, no. 6 (1997): 345–52. http://dx.doi.org/10.1007/s002510050215.

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28

Verma, N., and P. Reeves. "Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae." Journal of Bacteriology 171, no. 10 (1989): 5694–701. http://dx.doi.org/10.1128/jb.171.10.5694-5701.1989.

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29

Yazdanbakhsh, Amir, Gennady Pekhimenko, Bradley Thwaites, Hadi Esmaeilzadeh, Onur Mutlu, and Todd C. Mowry. "RFVP." ACM Transactions on Architecture and Code Optimization 12, no. 4 (2016): 1–26. http://dx.doi.org/10.1145/2836168.

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30

Jayaratne, P., D. Bronner, P. R. MacLachlan, C. Dodgson, N. Kido, and C. Whitfield. "Cloning and analysis of duplicated rfbM and rfbK genes involved in the formation of GDP-mannose in Escherichia coli O9:K30 and participation of rfb genes in the synthesis of the group I K30 capsular polysaccharide." Journal of Bacteriology 176, no. 11 (1994): 3126–39. http://dx.doi.org/10.1128/jb.176.11.3126-3139.1994.

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31

Marolda, C. L., and M. A. Valvano. "Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene." Journal of bacteriology 177, no. 19 (1995): 5539–46. http://dx.doi.org/10.1128/jb.177.19.5539-5546.1995.

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32

Ward, Teresa R., Margaret L. Hoang, Reeta Prusty, et al. "Ribosomal DNA Replication Fork Barrier and HOT1Recombination Hot Spot: Shared Sequences but Independent Activities." Molecular and Cellular Biology 20, no. 13 (2000): 4948–57. http://dx.doi.org/10.1128/mcb.20.13.4948-4957.2000.

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ABSTRACT In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3′ of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to thecis-acting, mitotic hyperrecombination site calledHOT1. We have found that the RFB and HOT1activity share some but not all of their essential sequences. Many of the mutations that reduce HOT1 recombination also decrease or eliminate fork arrest at one of two closely spaced RFB sites, RFB1 and RFB2. A simple model for the juxtaposition of RFB and

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Sturm, S., and K. N. Timmis. "Cloning of the rfb gene region of Shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines." Microbial Pathogenesis 1, no. 3 (1986): 289–97. http://dx.doi.org/10.1016/0882-4010(86)90054-9.

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Gedeon, A. K., J. C. Mulley, and A. Fratini. "AnMsp1 RFLP atD16S10." Nucleic Acids Research 17, no. 5 (1989): 2146. http://dx.doi.org/10.1093/nar/17.5.2146.

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Garcia, P. M., E. F. Arcuri, M. A. V. P. Brito, C. C. Lange, J. R. F. Brito, and M. M. O. P. Cerqueira. "Detecção de Escherichia coli O157:H7 inoculada experimentalmente em amostras de leite cru por método convencional e PCR multiplex." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 60, no. 5 (2008): 1241–49. http://dx.doi.org/10.1590/s0102-09352008000500029.

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Padronizou-se um método de reação em cadeia da polimerase (PCR) multiplex para detecção de Escherichia coli O157:H7 e avaliou-se a eficiência da PCR e de um método de cultivo convencional em placas na detecção desse patógeno experimentalmente adicionado em leite estéril e em leite cru com baixa contagem bacteriana total (média de 4,01 x 10³ UFC/ml) e com alta contagem bacteriana (média de 2,10 x 10(6) UFC/ml). Foram padronizadas duas reações de PCR com o uso dos primers: "A" (RfbF; RfbR e FLICh7F/FLICh7R) e "B" (SLT-IF/SLTIR e SLT-IIF/SLT-IIR). A detecção de E. coli O157:H7 (1UFC/ml) a partir

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Murakami, Masahiro, Toshiya Matsuzaki, Takeshi Iwasa, et al. "Hypophysiotropic role of RFamide-related peptide-3 in the inhibition of LH secretion in female rats." Journal of Endocrinology 199, no. 1 (2008): 105–12. http://dx.doi.org/10.1677/joe-08-0197.

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Gonadotropin-inhibitory hormone (GnIH), a newly discovered hypothalamic RFamide peptide, inhibits reproductive activity by decreasing gonadotropin synthesis and release in birds. The gene of the mammalian RFamide-related peptides (RFRP) is orthologous to the GnIH gene. This Rfrp gene gives rise to the two biologically active peptides RFRP-1 (NPSF) and RFRP-3 (NPVF), and i.c.v. injections of RFRP-3 suppress LH secretion in several mammalian species. In this study, we show whether RFRP-3 affects LH secretion at the pituitary level and/or via the release of GnRH at the hypothalamus in mammals. To

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Stowe, Melinda. "To RFP or Not to RFP." Journal of Library Administration 26, no. 3-4 (1999): 53–74. http://dx.doi.org/10.1300/j111v26n03_04.

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Pina, Cara, and Francesca Pignoni. "Tubby-RFP balancers for developmental analysis: FM7c 2xTb-RFP, CyO 2xTb-RFP, and TM3 2xTb-RFP." genesis 50, no. 2 (2012): 119–23. http://dx.doi.org/10.1002/dvg.20801.

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Hirose, K., K. I. Itoh, H. Nakajima, et al. "Selective Amplification of tyv (rfbE), prt (rfbS), viaB, and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A." Journal of Clinical Microbiology 40, no. 2 (2002): 633–36. http://dx.doi.org/10.1128/jcm.40.02.633-636.2002.

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40

Poling, Matthew C., Joshua Kim, Sangeeta Dhamija, and Alexander S. Kauffman. "Development, Sex Steroid Regulation, and Phenotypic Characterization of RFamide-Related Peptide (Rfrp) Gene Expression and RFamide Receptors in the Mouse Hypothalamus." Endocrinology 153, no. 4 (2012): 1827–40. http://dx.doi.org/10.1210/en.2011-2049.

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Arginine-phenylalanine-amide (RFamide)-related peptide 3 (RFRP-3, encoded by the Rfrp gene) is the mammalian ortholog of gonadotropin-inhibiting hormone and can inhibit GnRH neuronal activity and LH release. However, the development and regulation of the RFRP-3 system in both sexes is poorly understood. Using in situ hybridization, we examined changes in Rfrp-expressing neurons in mice of both sexes during development and under different adulthood hormonal milieus. We found no sex differences in Rfrp expression or cell number in adult mice. Interestingly, we identified two interspersed subpopu

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Rahdar, Parastoo, and Homayoun Khazali. "Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats." International Journal of Basic Science in Medicine 4, no. 1 (2019): 4–9. http://dx.doi.org/10.15171/ijbsm.2019.02.

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Introduction: It is well-established that gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologues (RFRP: RF-amid related peptides) can decrease gonadotrophin secretion. Moreover, substance P (SP) is another modulator of the secretion of gonadotropins in a species-dependent manner. This study aimed to find out the impacts of concomitant infusion of RFRP-3 and SP on luteinizing hormone (LH) concentration. Methods: Forty-two rats were arbitrarily assigned to 7 groups (n=6 per group). Animals in the experimental groups were intracerebroventricularly injected with saline +DMSO, SP, RFR

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ASHER, A. J., L. S. WALDRON, and M. L. POWER. "Rapid identification of Giardia duodenalis assemblages in NSW using terminal-restriction fragment length polymorphism." Parasitology 139, no. 8 (2012): 1005–13. http://dx.doi.org/10.1017/s0031182012000388.

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SUMMARYHumans are infected by 2 genetic assemblages (A and B) of Giardia duodenalis, a protozoan parasite that causes gastro-intestinal disease. Sub-assemblages AI, AII, BIII and BIV are commonly identified in human cases. Detection requires amplification of G. duodenalis loci. Subsequent DNA sequencing or restriction fragment length polymorphism (RFLP) identifies sub-assemblages but is expensive (DNA sequencing) or insensitive (RFLP). This study investigated a fluorescence-based detection method, using terminal-restriction fragment length polymorphism (T-RFLP) of the glutamate dehydrogenase g

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VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe

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Jørgensen, Sara R., Mille D. Andersen, Agnete Overgaard, and Jens D. Mikkelsen. "Changes in RFamide-Related Peptide-1 (RFRP-1)-Immunoreactivity During Postnatal Development and the Estrous Cycle." Endocrinology 155, no. 11 (2014): 4402–10. http://dx.doi.org/10.1210/en.2014-1274.

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Abstract GnRH is a key player in the hypothalamic control of gonadotropin secretion from the anterior pituitary gland. It has been shown that the mammalian counterpart of the avian gonadotropin inhibitory hormone named RFamide-related peptide (RFRP) is expressed in hypothalamic neurons that innervate and inhibit GnRH neurons. The RFRP precursor is processed into 2 mature peptides, RFRP-1 and RFRP-3. These are characterized by a conserved C-terminal motif RF-NH2 but display highly different N termini. Even though the 2 peptides are equally potent in vitro, little is known about their relative d

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Rahdar, Parastoo, and Homayoun Khazali. "Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats." International Journal of Basic Science in Medicine 3, no. 3 (2018): 127–32. http://dx.doi.org/10.15171/ijbsm.2018.23.

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Background: Gonadotropin-inhibitory hormone and its mammalian orthologues (RFRP: RF amid related peptide) are known to inhibit the secretion of gonadotropins. In addition, substance P (SP) a member of tachykinin’s family is demonstrated that can increase the firing rate of kisspeptin/neurokinin B/dynorphin (KNDy) neurons and provokes the of secretion gonadotropins. In this experimental study we investigated the effects of co-administration of RFRP-3 and SP on the expression of KISS-1 and GnRH genes in male rats. Methods: forty-two mature Wistar rats were randomly allocated in 7 groups (n=6 in

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Xiong, Xianrong, Yulei Hu, Bangting Pan, et al. "RFRP-3 Influences Apoptosis and Steroidogenesis of Yak Cumulus Cells and Compromises Oocyte Meiotic Maturation and Subsequent Developmental Competence." International Journal of Molecular Sciences 24, no. 8 (2023): 7000. http://dx.doi.org/10.3390/ijms24087000.

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RF amide-related peptide 3 (RFRP-3), a mammalian ortholog of gonadotropin-inhibitory hormone (GnIH), is identified to be a novel inhibitory endogenous neurohormonal peptide that regulates mammalian reproduction by binding with specific G protein-coupled receptors (GPRs) in various species. Herein, our objectives were to explore the biological functions of exogenous RFRP-3 on the apoptosis and steroidogenesis of yak cumulus cells (CCs) and the developmental potential of yak oocytes. The spatiotemporal expression pattern and localization of GnIH/RFRP-3 and its receptor GPR147 were determined in

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Stalker, H. T., J. S. Dhesi, and G. Kochert. "Genetic diversity within the species Arachis duranensis Krapov. &W.C. Gregory, a possible progenitor of cultivated peanut." Genome 38, no. 6 (1995): 1201–12. http://dx.doi.org/10.1139/g95-158.

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Eighteen accessions of a diploid wild peanut species (Arachis duranensis) were analyzed using morphological, intercrossing, cytological, and RFLP data. Abundant variation was found for morphological characters and for RFLP patterns both between and within accessions, and each accession could be uniquely identified by RFLP pattern. Several plants were found to be F1 hybrids between different accessions, indicating that intercrossing had occurred when these were planted for seed increase. Patterns of RFLP diversity were found to correspond with geographic distribution. Analysis of the number of

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Molnár, C. S., I. Kalló, Z. Liposits, and E. Hrabovszky. "Estradiol Down-Regulates RF-Amide-Related Peptide (RFRP) Expression in the Mouse Hypothalamus." Endocrinology 152, no. 4 (2011): 1684–90. http://dx.doi.org/10.1210/en.2010-1418.

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Abstract In most mammals, RF-amide-related peptides are synthesized in the dorsomedial hypothalamic nucleus and regulate reproduction via inhibiting GnRH neurons and, possibly, adenohypophyseal gonadotrophs. In the present study, we investigated the possibility that RFRP-synthesizing neurons are involved in estrogen feedback signaling to the reproductive axis in mice. First, we used quantitative in situ hybridization and compared the expression of prepro-RFRP mRNA of ovariectomized mice, with and without 17β-estradiol (E2) replacement. Subcutaneous administration of E2 via silastic capsules fo

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Pate, M., M. Moravkova, B. Krt, I. Pavlik, and M. Ocepek. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP." Veterinární Medicína 54, No. 6 (2009): 270–79. http://dx.doi.org/10.17221/3084-vetmed.

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ABSTRACT: Apart from birds, <I>Mycobacterium avium</I> subsp. <I>avium (MAA) </I> is often isolated from granulomatous lesions in pigs and occasionally from cattle and other animals. The objectives of this study were the detection of IS<I>901</I> restriction fragment length polymorphism (RFLP) types of <I>MAA</I> isolates from different species of domestic animals between the years 1998 and 2004 and the comparison of the detected RFLP types with previously described RFLP types collected in the database of the OIE Reference Laboratory for Avian Tu

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Squicciarini, V., R. Riquelme, K. Wilsterman, G. E. Bentley, and H. E. Lara. "Role of RFRP-3 in the development of cold stress-induced polycystic ovary phenotype in rats." Journal of Endocrinology 239, no. 1 (2018): 81–91. http://dx.doi.org/10.1530/joe-18-0357.

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RFamide-related peptide (RFRP-3) is a regulator of GnRH secretion from the brain, but it can also act in human ovary to influence steroidogenesis. We aimed to study the putative local role of RFRP-3 in the ovary and its potential participation in the development of a polycystic ovary phenotype induced by chronic sympathetic stress (cold stress). We used adult Sprague–Dawley rats divided into control and stressed groups. In both groups, we studied the effect of intraovarian exposure to RFRP-3 on follicular development and plasma ovarian steroid concentrations. We also tested the effect of RFRP-

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