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Dissertations / Theses on the topic 'RFLP[Restriction fragment length polymorphism assay]'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Vasilache, Gouandjika Ionela. "Circulation et dérive génétique du poliovirus en Afrique Centrale et de l'ouest." Paris 6, 2006. http://www.theses.fr/2006PA066222.

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Gray, Stephen James. "The genotyping of Neisseria meningitidis by restriction fragment length polymorphism (RFLP) analysis." Thesis, Manchester Metropolitan University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360085.

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3

Gysling, Kevin. "Polymerase chain reaction restriction fragment length polymorphism identification of trebouxia lichen photosymbionts." Honors in the Major Thesis, University of Central Florida, 2009. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1269.

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This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.<br>Bachelors<br>Medicine<br>Molecular Biology and Microbiology

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4

Bijelovic, Jelena. "Identification of mould and blue stain fungi on wood using Polymerase Chain Reaction and Terminal Restriction Fragment Length Polymorphism." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7100.

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<p>Wood inhabiting fungi oposes a great problem for preservation of wooden surfaces everywhere, being the main problem of economic losses of wooden products.</p><p>A reference collection consisting of 9 different genus constituting of 21 different strains of wood-inhabiting fungi was used for identification of unknown species of mould and blue stain fungi on wood. The fungus DNA from the samples was isolated from malt extract agar. PCR (Polymerase Chain Reaction) was conducted on rDNA ITS1 and ITS2 regions for amplification of the DNA. The 21 samples were collected to a reference collection fo

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Kirker, Grant Terral. "EFFECTS OF CHLOROTHALONIL AND BUTYLATED HYDROXYTOLUENE ON MICROBIAL COMMUNITIES INVOLVED IN THE DETERIORATION OF WOOD USING TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) ANALYSES." MSSTATE, 2008. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022008-155301/.

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The effects of an organic biocide (CTN) with and without co-added antioxidant (BHT) on microbial communities in SYP were assessed using terminal restriction fragment length polymorphism (T-RFLP) analyses in both field and accelerated decay laboratory studies. Ammoniacal copper quaternary (ACQ-C) was used as a positive control in the field study component, but not in the laboratory test. Field stakes were treated with 0.25 and 0.37% ammoniacal copper quat (ACQ-C), CTN (0.1 and 0.25%), CTN (0.1 and 0.25%) with 2% BHT added, 2% BHT alone, and controls were left untreated. In the field studies, pr

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Kirker, Grant Terral. "Effects of chlorothalonil (CTN) and butylated hydroxy-toluene (BHT) on microbial communities involved in the deterioration of wood using terminal restriction fragment length polymorphism (T-RFLP) analyses." Diss., Mississippi State : Mississippi State University, 2008. http://library.msstate.edu/etd/show.asp?etd=etd-04022008-155301.

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7

Pavan, Tycha Bianca Sabaini 1983. "Detecção do gene 16Sr-RNA e identificação de patógenos bacterianos, causadores de sepse neonatal precoce, pela técnica da RFLP-PCR ("Restriction Fragment Length Polymorfism - Polymerase Chain Reaction")." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/309724.

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Orientador: Sergio Tadeu Martins Marba<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas<br>Made available in DSpace on 2018-08-23T19:06:43Z (GMT). No. of bitstreams: 1 Pavan_TychaBiancaSabaini_M.pdf: 1135745 bytes, checksum: 87193a679a977d0df4230db53eab4297 (MD5) Previous issue date: 2013<br>Resumo: O objetivo do trabalho foi descrever e avaliar a capacidade da "RFLP-PCR" para detectar patógenos bacterianos em recém-nascidos (RN) e determinar seu uso diagnóstico na sepse neonatal precoce (SNP). Foram avaliados RNs assintomáticos, filhos de gestante

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8

Moreira, Abdiel Aparecido. ""Pesquisa de sítios de restrição enzimática em segmento da ORF K1 do genoma de herpesvírus humano tipo 8 (HHV-8) em isolados clínicos de São Paulo: relação com subtipos virais e implantação da técnica de RFLP (Restriction Fragment Length Polymorphism Analyses) para determinar subtipos virais"." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01102004-164856/.

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A epidemia da Síndrome de Imunodeficiência Adquirida (AIDS) fez aumentar a incidência de sarcoma de Kaposi (SK) em todos os países e o SK passou a ser considerado doença definidora de AIDS. Desde a descoberta de seu agente etiológico, o herpesvírus humano tipo 8 (HHV-8), vários estudos vêm sendo realizados com o objetivo de caracterizar subtipos virais presentes em todas as formas de SK: clássica, endêmica, iatrogênica e epidêmica. Os sistemas rotineiramente usados na subtipagem do HHV-8 têm usado o seqüenciamento de um gene que contém regiões hipervariáveis e que codifica uma glicoproteína de

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9

Chikaha, Tchouya-Ngandjio Antoinette Brigitte. "Epidémiologie des infections urogénitales à Chlamydia trachomatis chez des étudiants camerounais (Yaoundé) : génotypage des souches, sensibilité aux antibiotiques : mise au point de la détection quantitative par PCR en temps réel." Paris 6, 2003. http://www.theses.fr/2003PA066055.

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10

Corniquel, Béatrice. "Construction de banques d'ADNc de palmier dattier (Phoenix dactylifera L. ). Différenciation de cultivars par RFLP à l'aide des ADNc : isolement et caractérisation d'un ADNc polymorphe. Isolement et caractérisation d'un ADNc codant pour la glutamine synthetase cytosolique." Angers, 1994. http://www.theses.fr/1994ANGE0014.

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La caractérisation des cultivars de palmier dattier a été réalisée par des analyses RFLP et RAPD à partir des feuilles internes des rejets. Des sondes ont été isolées d'une banque d’ADNc construite à partir d'ARNM extraits de cals organogènes du cultivar bou sthammi noire. La sonde ADNc 1 a permis d'obtenir neuf profils d'hybridation distincts pour chacun des neuf cultivars étudiés. Des profils d'amplification distincts pour trois cultivars ont été obtenus par RAPD avec des amorces oligonucléotidiques. Des analyses RFLP ont été aussi réalisées sur du matériel maintenu in vitro ou issu de cultu

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11

Chung, Kuo-Fang, and 鍾國芳. "Phylogenetic inference of Genus Nicotiana L. based on restriction fragment length polymorphism (RFLP)." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/73638761006658100848.

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碩士<br>國立臺灣大學<br>植物學系<br>84<br>Using three cDNA clones and twenty-six genomic clones of Ni- cotiana plumbaginfolia, restriction fragment length polymor- phisms (RFLPs) of nuclear DNA were studies to infer the inter- and intrasectional phylogenetic relationship of section Alatae and section Tomentosae of genus Nicotiana L.. By using these RFLPs markers as discrimination characters, phylogenetic trees were obtain based on parsimony and distance matrix methods of PHYLIP package. The taxonomic h

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Lin, Huei-Ming, and 林徽鳴. "Monitoring molecular fingerprinting of microbial community dynamics by denaturing gradient gel electrophoresis(DGGE) and terminal-restriction fragment length polymorphism (T-RFLP)." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/56892104243407930869.

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碩士<br>國立成功大學<br>生命科學系碩博士班<br>93<br>Soil bacteria are essential components of the biotic community in natural forests and they are largely responsible for ecosystem function, and participate in the elements circulation. Although the main diversity of life has been proven to be microbial, the vast majority of soil bacteria still remain unknown for less than 1% of environmental microorganisms can be cultured. In this study, cloning method, denaturing gradient gel electrophoresis (DGGE) and terminal-restriction fragment length polymorphism (T-RFLP) techniques were used to compare the prevalent mic

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13

Iranpour, Mahmood. "Egg parasitoids of tabanids in Manitoba : prevalence, taxonomy, behaviour, and use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to establish host-parasite interrelationships." 2003. http://hdl.handle.net/1993/7820.

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Tabanid egg masses were collected from different locations in Manitoba (Canada) during the summers of 1996-2000. Two scelionid parasitoids (Telenomus species A and B) were reared from horse fly egg masses (Hybomitra spp.) and another scelionid parasitoid (Telenomus species C) along with one trichogrammatid parasitoid (Trichogramma semblidis (Aurivillius)) were reared from deer fly egg masses (Chrysops spp.). The taxonomy and host-associations of ten Telenomus species recorded from eggs of Tabanidae in different geographical regions of the world were reviewed and type specimens of these species

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14

Kernaghan, Shaun. "Characterization of the Bacterial Communities of the Tonsil of the Soft Palate of Swine." Thesis, 2013. http://hdl.handle.net/10214/7750.

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Terminal restriction fragment length polymorphism (T-RFLP) analysis and pyrosequencing were used to characterize the microbiota of the tonsil of the soft palate of 126 unfit and 18 healthy pigs. The T-RFLP analysis method was first optimized for the study of the pig tonsil microbiota and the data compared with culture-based identification of common pig pathogens. Putative identifications of the members of the microbiota revealed that the phyla Firmicutes, Proteobacteria and Bacteroidetes were the most prevalent. A comparison of the T-RFLP analysis results grouped into clusters to clinical cond

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Kabahuma, Rosemary I. "Genetic aspects of hearing loss in the Limpopo Province of South Africa." Thesis, 2010. http://hdl.handle.net/10539/8566.

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The aetiological diagnosis of recessive non-syndromic hearing loss poses a challenge owing to marked heterogeneity and the lack of identifying clinical features. The finding that up to 50% of recessive non-syndromal genetic hearing loss among Caucasians was due to mutations in GJB2, the gene encoding Connexin 26 (Cx26) was a breakthrough, whose value as a diagnostic tool has been limited by the significant variation in the prevalence of deafness genes and loci among population groups. The significant association of the GJB6-D13S1830 deletion among individuals with one mutant GJB2 allele

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16

Heller, Samuel. "Aldosteron syntáza u arteriální hypertenze a možný vliv polymorfismu jejího genu na hypertrofii levé komory srdeční." Doctoral thesis, 2013. http://www.nusl.cz/ntk/nusl-326710.

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Part I. The aldosterone synthase gene (CYP11B2) polymorphism T-344C in blood pressure and left ventricular hypertrophy. BACKGROUND: Aldosterone is a key cardovascular hormone, it significantly influences volume, pressure and electrolyte balance. Aldosterone plays an important role in development of left ventricular (LV) hypertrophy and myocardial fibrosis. The aldosterone synthase gene (CYP11B2) is an important candidate gene region in essential hypertension. DESIGN AND METHODS: We assessed the influence of the T-344C polymorphism of aldosterone synthase - the rate-limiting enzyme in aldostero

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