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Dissertations / Theses on the topic 'RFLP'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after rep

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2

Olivová, Radana. "Optimalizace metody PCR-RFLP pro taxonomické zařazení kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216560.

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This thesis deal with optimalization method PCR – RFLP for taxonomy enlistment of yeasts. Conventional identification methods for yeasts are time-consuming. Molecular biological method based on PCR are instrumental towards fast and precise identification as compared to conventional phenotypic methods. In this thesis molecular biological method PCR – RFLP was used for identification and enlistment of yeasts. This metod follow repeating spacers of ribozomal DNA of yeast, characteristic for each species and strain. By the help of PCR were amplified specific partitions of DNA. These fragments of D

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3

Wolf, Markus. "Markergestützte Vererbungsanalyse der Pollenfertilitätsrestauration bei Winterroggen (Secale cereale L.)." [S.l. : s.n.], 2001. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9818604.

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4

Cavell, Jane Sarah. "Cytogenetic and RFLP analyses of somaclonal variation in Nicotiana tabacum." Thesis, University of Bath, 1989. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.328517.

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5

Datta, Krishna. "Wide hybridisation and isozyme, RAPD and RFLP markers of #Corchorus' species." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283339.

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6

Dreyer, Felix. "Chromosomale Lokalisation von Restorergenen argentinischer und iranischer Herkunft in F2-Populationen von Winterroggen /." Aachen : Shaker, 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009029454&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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7

SANTOS, Lorena Cristina. "Caracterização molecular de Mycobacterium tuberculosis isolados de pacientes atendidos na cidade de Goiânia-GO, pela técnica de RFLP-IS6110." Universidade Federal de Goiás, 2008. http://repositorio.bc.ufg.br/tede/handle/tde/1826.

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Made available in DSpace on 2014-07-29T15:30:41Z (GMT). No. of bitstreams: 1 Dissertacao Lorena Cristina.pdf: 430117 bytes, checksum: 09642b3a4726659137a90dcbf634ba90 (MD5) Previous issue date: 2008-02-29<br>Tuberculosis is a serious public health problem all over the world. It has been demonstrated that different M. tuberculosis strains, characterized by the RFLP-IS6110 standard technique, have different virulence properties and antibiotic resistance. The aim of this study was to characterize M. tuberculosis strains isolated from patients attending two state reference hospitals of Goiânia-G

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8

Ip, Ka-fai. "Molecular epidemiological study of mycobacterium tuberculosis using IS6110-RFLP and MIRU typing /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31495473.

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9

Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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10

Ip, Ka-fai, and 葉嘉輝. "Molecular epidemiological study of mycobacterium tuberculosis using IS6110-RFLP and MIRU typing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B45010092.

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11

Gray, Stephen James. "The genotyping of Neisseria meningitidis by restriction fragment length polymorphism (RFLP) analysis." Thesis, Manchester Metropolitan University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360085.

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12

LU, YUN-HAI. "Diversite moleculaire (rflp) et cartographie genomique chez la canne a sucre (saccharumspp. )." Paris 11, 1992. http://www.theses.fr/1992PA112398.

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Les varietes modernes de canne a sucre (saccharum) sont aneuploides avec souvent plus de 100 chromosomes, qui proviennent en majorite (>80) de s. Officinarum et dans une moindre mesure des especes sauvages, surtout de s. Spontaneum l'utilisation des marqueurs genetiques moleculaires (rflp, restriction fragment length polymorphism), bien que delicate chez une plante si complexe, a ete developpee, avec pour objectifs la connaissance des ressources genetiques et l'exploitation des possibilites de cartographie moleculaire du genome. La diversite a ete analysee parmi une cinquantaine de clones repr

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13

LAURENT, VALERIE. "Etude de la diversite genetique du cacaoyer a l'aide des marqueurs rflp." Paris 11, 1993. http://www.theses.fr/1993PA112110.

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L'espece theobroma cacao est originaire de l'amerique tropicale. Elle est divisee en trois groupes, criollo, forastero et trinitario, sur la base des caracteres morphologiques et de l'origine geographique des clones. Cette classification ne se revele pas toujours appropriee pour l'identification des clones et le choix des parents pour les croisements. Une etude de la diversite de l'adn de cacaoyer a ete realisee a l'aide des marqueurs rflp. La variabilite de l'adn ribosomique nucleaire, de l'adn mitochondrial et des sequences d'adn complementaire confirme la structuration de l'espece en trois

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14

Malvárez, Macedo Gabriela. "Aplicação das metodologias PCR/RFLP para caracterização de fungos ectomicorrízicos em eucalipto /." reponame:Repositório Institucional da UFSC, 1999. https://repositorio.ufsc.br/handle/123456789/112290.

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15

Chitnis, P. K. "Comparative study of Indica and Japonica rices using RFLP and morphological characters." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1992. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3038.

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16

Rocha, Lidianne Leal. "Estudo de comunidades bacterianas de solos do Manguezal da Barra Grande, IcapuÃ â CE e SeleÃÃo de cepas com potencial para degradar hidrocarbonetos." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2773.

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Os manguezais sÃo ecossistemas entremarÃs produtivos e biologicamente importantes que ocorrem em regiÃes tropicais e subtropicais do mundo. Ãreas de manguezais nÃo perturbados fornecem habitats para uma variedade de plantas, animais e microrganismos. Estes ecossistemas recebem materiais sedimentares do mar e continente, tornando-se uma Ãrea de transiÃÃo com elevada produtividade. Importantes processos, tais como ciclagem de nutrientes, estÃo diretamente conectados Ã atividade e diversidade das comunidades microbianas dos solos do manguezal. No entanto, devido a sua localizaÃÃo estratÃgica, man

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17

Mendes, Lucas William. "Análise molecular das estruturas e diversidade de comunidades microbianas em solo de manguezal preservado da Ilha do Cardoso-SP." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-27042010-112316/.

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Os manguezais tropicais são considerados um dos ecossistemas mais produtivos do mundo, sendo caracterizados pela alta taxa de ciclagem de matéria orgânica e nutrientes que ocorre entre os oceanos e os ambientes terrestres. Embora os manguezais sejam considerados áreas de proteção ambiental, a destruição desses ambientes é progressiva, devido a atividades industriais e portuárias nos estuários. Nos manguezais, a ciclagem de nutrientes está diretamente relacionada às atividades e a diversidade das comunidades microbianas presentes no solo. Este trabalho está inserido em um projeto mais amplo den

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18

Naamala, J., SK Jaiswal, and FD Dakora. "Microsymbiont diversity and phylogeny of native bradyrhizobia associated with soybean (Glycine max L. Merr.) nodulation in South African soils." Systematic and Applied Microbiology, 2016. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001977.

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Abstract The genetic diversity and identification of slow- and fast- growing soybean root nodule bacterial isolates from different agro-climatic regions in Mpumalanga, Limpopo and Gauteng Provinces of South Africa were evaluated. The 16S-rDNA-RFLP analysis of 100 rhizobial isolates and eight reference type strains placed the isolates into six major clusters, and revealed their site-dependent genomic diversity. Sequence analysis of single and concatenated housekeeping genes (atpD, glnII and gyrB), as well as the symbiotic gene nifH captured a considerably higher level of genetic diversity and i

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19

Fiore, Rebecca de Ara?jo. "Potencial de esp?cies florestais para remedia??o de substrato contaminado com atrazine e 2,4-D." UFVJM, 2014. http://acervo.ufvjm.edu.br:8080/jspui/handle/1/338.

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Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-05T18:42:52Z No. of bitstreams: 2 rebeca_araujo_fiore.pdf: 1355098 bytes, checksum: 9068f2cf748b48bb87c50d51c7cdea53 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)<br>Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-05T18:43:21Z (GMT) No. of bitstreams: 2 rebeca_araujo_fiore.pdf: 1355098 bytes, checksum: 9068f2cf748b48bb87c50d51c7cdea53 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5)<br>Approved for entry

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MEDEIROS, Rafael Acioli. "Caracterização da Leishmania infantum e Leishmania (Viannia) braziliensis em cães provenientes da Região Metropolitana do Recife, Pernambuco." Universidade Federal de Pernambuco, 2013. https://repositorio.ufpe.br/handle/123456789/12218.

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Submitted by Milena Dias (milena.dias@ufpe.br) on 2015-03-12T17:45:21Z No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5)<br>Made available in DSpace on 2015-03-12T17:45:21Z (GMT). No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013<br>CAPES<br>As Leishmanioses são uma antropozoonose co

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21

Björkbom, Tommy. "PCR-RFLP analys av mt-DNA hos Öring (Salmo trutta) i Gävleborgs län." Thesis, University of Gävle, Department of Electronics, Mathematics and Natural Sciences, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-7624.

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22

Kleiner, Sven. "Entwerfen und Entwickeln mit Systems Engineering auf Basis des RFLP-Ansatzes in V6." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-228610.

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Der Begriff Systems Engineering taucht in letzter Zeit verstärkt in Verbindung mit der Entwicklung, dem Testen und Validieren von technischen Systemen auf (Alt 2012, Sendler 2012, Stark 2012). Die Gesellschaft für Systems Engineering GfSE beschreibt Systems Engineering als eine umfassende Ingenieurtätigkeit, die zur Entwicklung komplexer Produkte notwendig ist (GfSE 2012). Neben den klassischen Anwendungsgebieten, der Luft- und Raumfahrt, gewinnt diese Methodik auch in weiteren Bereichen, wie z.B. der Automobilbranche oder der Medizintechnik, immer mehr an Bedeutung. In diesem Zusammenhang die

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23

Sultan, Amal Hassan. "The development of molecular tools for identification of Leishmania species by PCR-RFLP." Thesis, University of Liverpool, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479067.

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24

Kleiner, Sven. "Entwerfen und Entwickeln mit Systems Engineering auf Basis des RFLP-Ansatzes in V6." TUDpress - Verlag der Wissenschaften GmbH, 2012. https://tud.qucosa.de/id/qucosa%3A30518.

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Der Begriff Systems Engineering taucht in letzter Zeit verstärkt in Verbindung mit der Entwicklung, dem Testen und Validieren von technischen Systemen auf (Alt 2012, Sendler 2012, Stark 2012). Die Gesellschaft für Systems Engineering GfSE beschreibt Systems Engineering als eine umfassende Ingenieurtätigkeit, die zur Entwicklung komplexer Produkte notwendig ist (GfSE 2012). Neben den klassischen Anwendungsgebieten, der Luft- und Raumfahrt, gewinnt diese Methodik auch in weiteren Bereichen, wie z.B. der Automobilbranche oder der Medizintechnik, immer mehr an Bedeutung. In diesem Zusammenhang die

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25

Besse, Pascale. "Identification clonale et analyse de la diversité génétique chez hevea brasiliensis par RFLP." Paris 11, 1993. http://www.theses.fr/1993PA112124.

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Hevea brasiliensis est un arbre originaire d'amerique du sud. Des techniques de marquage moleculaire par rflp ont ete developpees afin d'appuyer les programmes de selection. Le developpement de la technique d'empreintes genetiques a permis d'identifier les 73 clones cultives wickman etudies. Une etude de diversite a ete entreprise afin d'appuyer la gestion des ressources genetiques d'hevea (comparaison clones cultives et individus prospectes au bresil dans l'acre, le rondonia et le mato-grosso). L'etude de l'adn ribosomique a permis d'emettre l'hypothese de l'existence de deux sous-races dans

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26

SZABO, VERONIQUE. "Cartographie des genes qui differencient mais et teosinte a l'aide de marqueurs rflp." Paris 11, 1991. http://www.theses.fr/1991PA112406.

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La segregation des caracteres clefs qui differencient mais et teosinte a ete analysee dans trois populations f#2 et trois backcross entre la lignee de mais t232 et zea mays ssp. Parviglumis. Ces caracteres sont: epillets femelles par paire chez le mais, distiches chez la teosinte, epis femelles et glumes de l'epi femelle indurees chez la teosinte. Les autres caracteres etudies sont l'inclinaison des grains par rapport a l'axe du rachis, la polystichie (respectivement distichie) du mais (respectivement teosinte) et la date de floraison. 50 marqueurs rflp couvrant la totalite du genome ont ete u

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27

Grain, Françoise. "Étude des gènes du complexe majeur d'histocompatibilité : typage d'ovins par la méthode RFLP." Lyon 1, 1994. http://www.theses.fr/1994LYO1T183.

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28

Santana, Maiele Cintra. "Análise da comunidade de fungos em áreas de monoculturas e consórcio de Eucalyptus grandis e Acacia mangium." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-03052018-173930/.

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Os fungos representam cerca de 75% da biomassa microbiana em áreas florestais, desempenhando funções importantes, desde a mineralização dos resíduos orgânicos até a disponibilização de nutrientes para plantas por meio das associações micorrízicas, o que influencia a ciclagem de nutrientes e, consequentemente, o crescimento das árvores. O objetivo desse trabalho foi avaliar a comunidade de fungos do solo, da rizosfera e do sistema radicular de Eucalyptus grandis e Acacia mangium plantados em monocultivos e em consórcio, e encontrar respostas para os padrões observados por meio da correlação com

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29

Abreu, Daniel Paiva Barros de. "Caracteriza??o fenot?pica, genot?pica e perfil de sensibilidade a antif?ngicos de isolados cl?nicos de c?es e gatos pertencentes ao Complexo Sporothrix schenckii oriundos do estado do Rio de Janeiro." Universidade Federal Rural do Rio de Janeiro, 2017. https://tede.ufrrj.br/jspui/handle/jspui/2031.

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Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-13T12:31:48Z No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5)<br>Made available in DSpace on 2017-09-13T12:31:48Z (GMT). No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5) Previous issue date: 2017-02-20<br>Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq<br>Dimorphic fungi belonging to Sporothrix schenckii complex are responsible for sporotrichosis, importa

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30

Burger, Joachim A. "Sequenzierung, RFLP-Analyse und STR-Genotypisierung alter DNA aus archäologischen Funden und historischen Werkstoffen." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961822848.

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31

Miao, Yu. "Development and use of T-RFLP for studies of take-all infection of wheat." Thesis, University of Nottingham, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490991.

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Take-all of wheat is caused by the soil-borne fungus Gaeumannomyces graminis var. tritici (Ggt), and is a widespread and destructive disease in temperate climates around the world where losses may be as high as 50%. Several practices are used to limit the disease on susceptible crops, including tillage, rotation, choice of variety, N fertilizer, and chemical and biological seed treatments. In relation to biological control, it is necessary to better understand the effects of different treatments on microbial dynamics in the rhizosphere and on roots.

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Spengler, C. J. "PCR-RFLP typification of microbes used in the production of a fermented fish product." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52397.

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Thesis (MScFoodSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: The preservation of various fresh fish products is achieved by either smoking, salting, canning, freezing or fermenting a highly perishable raw product. Since many of these facilities are not readily available, the use of fermentation as a means of preserving the product has been extensively practiced. However, the fermentation of fish is a time consuming practise and only by accelerating the process would it be possible to ensure the production of a more cost effective and readily available safe end-product. The q

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33

Okuda, Liria Hiromi. "Orthopoxvirus bovino: inquérito soroepidemiológico e caracterização de amostras pela técnica de PCR e RFLP." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-09122013-152339/.

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No Brasil, casos de doença exantemática em bovinos e humanos têm sido relatados em diversas regiões, cujo agente causal é o virus vaccinia pertencente ao gênero Orthopoxirus da família Poxviridae. Classicamente, a varíola bovina é causada pelo Cowpoxvirus, entretanto, outros Poxvirus, como o vírus vaccinia podem desenvolver sintomatologia clínica semelhante. Além de comprometer a cadeia produtiva de leite, uma vez que dificulta a ordenha, predispõe a mastite e descarte do produto, é uma zoonose e atualmente está incluída no diagnóstico de doença vesicular. A origem desses casos bem como a epid

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Alvarez, Vega Luis Guillermo. "Detección de Staphylococcus pseudintermedius y Staphylococcus aureus aislados de piodermias caninas mediante PCR-RFLP." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2019. https://hdl.handle.net/20.500.12672/11838.

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A nivel mundial la piodermia es una de las enfermedades de la piel más diagnosticada en caninos. Entre los agentes involucrados se encontraba Staphylococcus intermedius, el que se creía el principal agente de las piodermias caninas; sin embargo, en el año 2005 fue reclasificado en 3 especies: S. intermedius, S. pseudintermedius y S. delphini. Posteriormente, estudios en diferentes países reportaron que el Staphylococcus pseudintermedius es en realidad el agente bacteriano más frecuentemente aislado en piodermias, seguidamente surgieron los primeros reportes de aislados resistentes a met

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35

Georges, Agnès. "Chlamydia Trachomatis et tetracyclines : étude de souches récidivantes par PCR-RFLP du gène omp1." Bordeaux 2, 1994. http://www.theses.fr/1994BOR2P094.

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36

Pereira, Lucas Carlos Gomes. "Importância dos polimorfismos C3435T e C1236T do gene de resistência a múltiplas drogas (MDR1) na resposta ao tratamento com mesilato de imatinib em pacientes com Leucemia Mielóide Crônica (LMC)." Universidade Federal de Goiás, 2013. http://repositorio.bc.ufg.br/tede/handle/tede/3100.

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Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2014-09-19T12:13:58Z No. of bitstreams: 2 Dissertacao Lucas C G Pereira.pdf: 869540 bytes, checksum: 9c40d119515c83b8b52606c52be6703d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)<br>Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-19T12:56:29Z (GMT) No. of bitstreams: 2 Dissertacao Lucas C G Pereira.pdf: 869540 bytes, checksum: 9c40d119515c83b8b52606c52be6703d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)<br>Made available in DSpace on

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Vitaliano, Sérgio Netto. "Isolamento e caracterização biológica e genotípica de Toxoplasma gondii em animais selvagens do Brasil." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04102012-182312/.

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Toxoplasma gondii é um protozoário formador de cistos capaz de infectar diversas espécies de aves e mamíferos domésticos e selvagens, incluindo o homem. Apesar de evidências sorológicas da infecção por T. gondii em animais selvagens, pouco se sabe sobre o papel da vida selvagem na cadeia epidemiológica e tampouco a susceptibilidade das variadas espécies a este parasito. O presente trabalho consistiu no isolamento e caracterização genotípica de T. gondii de tecidos de animais selvagens, de vida livre e de cativeiro, provenientes de diversas localidades do Brasil e na detecção sorológica de anti

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Andrade, Vanessa da Silva Silveira. "Polimorfismos genéticos, susceptibilidade e resposta ao tratamento em crianças portadoras de leucemia linfoblástica aguda." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-27082007-085802/.

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As leucemias constituem o câncer mais comum da infância, representando 30% de todas as neoplasias infantis. Dentre elas, a leucemia linfoblástica aguda (LLA) é a mais freqüente, atingindo 75% dos casos pediátricos de leucemias. A ocorrência da LLA tem sido relacionada com a exposição a alguns fatores ambientais (químico, físico e biológicos) e maternos (uso de drogas e dieta) tanto no desenvolvimento intra-útero como após o nascimento. No entanto, o processo de leucemogênese, particularmente com relação à importância da susceptibilidade genética herdade e fatores ambientais, ainda não foi eluc

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Costa, Nathalie Borges. "ANÁLISE DO POLIMORFISMO DO GENE CYP1A1m1 EM PACIENTES COM GLAUCOMA PRIMÁRIO DE ÂNGULO ABERTO DE UMA CLÍNICA OFTALMOLÓGICA EM GOIÂNIA, GO." Pontifícia Universidade Católica de Goiás, 2011. http://localhost:8080/tede/handle/tede/3425.

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Submitted by admin tede (tede@pucgoias.edu.br) on 2016-08-18T13:40:01Z No. of bitstreams: 1 Nathalie Borges Costa.pdf: 1419823 bytes, checksum: 9601be4b45901e83f91a699e9111d75f (MD5)<br>Made available in DSpace on 2016-08-18T13:40:01Z (GMT). No. of bitstreams: 1 Nathalie Borges Costa.pdf: 1419823 bytes, checksum: 9601be4b45901e83f91a699e9111d75f (MD5) Previous issue date: 2011-12-07<br>Glaucoma encompasses a group of eye diseases characterized by retinal ganglion cell damage, optical nerve damage and visual field loss. It is a complex optic neuropathy of genetically heterogeneity which can

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Fialová, Lenka. "Izolace a identifikace kvasinek z vinice pro jejich možné využití k výrobě vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240584.

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The aim of this work was to isolate the Saccharomyces cerevisiae yeast from the surface of wine grapes of the Malverina and Sauvignon varieties. Isolated yeasts were identified by the PCR-RFLP method. 5,8S ITS rDNA was amplified using PCR and restricition endonucleases HaeIII, HhaI, HinfI and TaqI were used for the restriction analysis which followed. The results were processed by UPGMA cluster analysis using the BioNumerics programme. The dominant genus on the surface of the Malverina variety grapes was Brettanomyces/Dekkera, while on the surface of the Sauvignon grapes we found mainly yeasts

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Arraes, Ana Carolina Palmeira. "Detecção da diversidade molecular de Candida spp. isoladas de UTI neonatal." reponame:Repositório Institucional da UFBA, 2013. http://www.repositorio.ufba.br/ri/handle/ri/11817.

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Submitted by Hiolanda Rêgo (hiolandar@gmail.com) on 2013-06-10T20:55:35Z No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5)<br>Made available in DSpace on 2013-06-10T20:55:35Z (GMT). No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5)<br>CAPES<br>A incidência de candidemia tem aumentado nos últimos anos e o espectro das espécies de Candida tem mudado com a emergência das espécies não-albicans e com o aumento da resistência antifúngica. A técn

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VINHAS, S. A. "ESTUDO SOBRE CARACTERÍSTICAS GENÉTICAS DE Mycobacterium tuberculosis ISOLADO DE PACIENTES COM E SEM LESÕES CAVITÁRIAS." Universidade Federal do Espírito Santo, 2013. http://repositorio.ufes.br/handle/10/4596.

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Made available in DSpace on 2016-08-29T15:35:05Z (GMT). No. of bitstreams: 1 tese_6777_Tese SAV 04-10-13.pdf: 4494409 bytes, checksum: 06e58e07b25332ca734da704af738a2c (MD5) Previous issue date: 2013-08-30<br>Introdução: Baseado na hipótese de que a variabilidade genética de Mycobacterium tuberculosis (MTB) pode influenciar a virulência e a gravidade da doença os perfis genéticos de isolados clínicos de MTB foram avaliados para detectar associação entre diversidade genética e gravidade da doença. Objetivos: Analisar características genéticas de isolados de MTB e verificar sua possível asso

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Michálek, Petr. "Sledování vlivu použité autochtonní kultury kvasinek na kvasný proces výroby vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217130.

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This thesis deals with the identification of wine yeasts isolated from the grape must using PCR-RFLP method. The yeasts were isolated from Pinot Noir grape variety must. Grapes were grown and produced in accordance with the requirements placed on organic and integrated farming. Samples were processed in the laboratory, where pure cultures of individual yeast were obtained. A commercial kit was used for yeast DNA isolation. Obtained DNA was used for further analysis. Using the polymerase chain reaction and the primers ITS1 and ITS4 a specific segment of 5.8S rDNA-ITS region was amplified. The P

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Marson, Erica Perez. "Caracterização da freqüência de heterozigose em genes ligados à precocidade sexual em novilhas de corte compostas." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-25072005-091446/.

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Os genes dos receptores do hormônio luteinizante (LHR) e folículo estimulante (FSHR), conhecidos por sua influência na manifestação da puberdade, foram avaliados por análise PCR-RFLP em uma população de 370 novilhas de corte compostas, de diferentes composições raciais Europeu-Zebu. Os objetivos foram caracterizar geneticamente a população investigada, utilizando-se de freqüências genotípicas e alélicas e estimativas de variabilidade e diversidade gênica; avaliar o efeito dos marcadores sobre a precocidade sexual, caracterizada pela probabilidade de prenhez por ocasião da primeira estação de m

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Rodakiewicz, Sheyla Michele. "Determinação da heterogeneidade do vírus da leucose bovina no estado de Santa Catarina." Universidade do Estado de Santa Catarina, 2013. http://tede.udesc.br/handle/handle/886.

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Made available in DSpace on 2016-12-08T16:24:14Z (GMT). No. of bitstreams: 1 PGCA13MA113.pdf: 865063 bytes, checksum: 41861f94a8b447b6d17dbc06ba954319 (MD5) Previous issue date: 2013-08-23<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Bovine leukemia virus (BLV) is a member of the family Retroviridae, genus Deltaretrovirus and it is an important agent, being primarily responsible for economic losses in dairy herds, causing losses of around 10% in production. The infection of BLV, can manifest itself in two ways, benign one, which reaches approximately 30% of the animals i

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陳子明 and Tsz-ming Chan. "Comparison of molecular epidemiological study on Mycobacterium tuberculosis using IS6110 RFLP and IS6110 PCR typing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31969689.

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Schmidt, Maren [Verfasser]. "Die Aussagekraft zusätzlicher RFLP-Analysen in defizienten Abstammungsbegutachtungen mittels Short-Tandem-Repeat-Analyse / Maren Schmidt." Kiel : Universitätsbibliothek Kiel, 2012. http://d-nb.info/1020283599/34.

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Fornazari, Anna Cristina Zari. "Determinação da comunidade microbiana pelo método molecular T-RFLP em carnes refrigeradas embaladas a vácuo." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-22112011-085341/.

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O estufamento de embalagens a vácuo de carnes refrigeradas, também conhecido como blown pack, é atribuído a bactérias psicrófilas e psicrotróficas, dentre as quais fazem parte algumas espécies de clostrídios, enterobactérias e bactérias lácticas. A capacidade desses microrganismos crescer em temperaturas de refrigeração adequadas torna um desafio o seu controle pela indústria. O Brasil é um dos grandes produtores de carne bovina no mundo e apesar da importância que a indústria de carne representa para o país, existem poucos estudos sobre as possíveis causas da deterioração do tipo blown pack.

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Paes, Fernanda Araujo. "AnÃlise de comunidades microbianas de solo de manguezal por T-RFLP e microarranjos de DNA." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2712.

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FundaÃÃo de Amparo Ã Pesquisa do Estado do CearÃ<br>The mangroves are essistemas important and productive, found along the coastline of tropical and subtropical regions. Important processes such as nutrient cycling, are directly connected to the activity of soil microbial communities. Given its strategic position the mangroves are very impacted the world. In Brazil, important areas such as the Bay of All Saints (BA) are severely affected by the presence of oil. Understanding the adaptation of the microbiota and its local dynamics, before the environmental change is essential to be able to main

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Baud, Sylvie. "Recherche de corrélations entre marqueurs moléculaires de type RFLP et caractères agronomiques chez Zea mays." Lyon 1, 1992. http://www.theses.fr/1992LYO10119.

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Dans le cadre d'un programme eureka, 170 marqueurs ont ete etudies a l'aide de l'outil rflp (restriction fragment length polymorphism) sur 35 lignees de mais. Les distances genetiques existant entre ces lignees, ont pu etre ainsi evaluees. La precision de l'outil, sa bonne representation de la variabilite existante entre les differents groupes genetiques du mais ont pu etre mis en evidence. Trois approches differentes de recherche de qtl (quantitative trait loci) ont, au prealable, ete comparees, a l'aide de donnees simulees: basees sur la regression phenotype sur genotype et considerant un ou

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