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Academic literature on the topic 'RGDP'

Author: Grafiati

Published: 11 September 2021

Last updated: 10 February 2022

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Journal articles on the topic "RGDP"

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Dienelly, Ummi, Samsul Bakri, and Trio Santoso. "Pengaruh Perubahan Tutupan Hutan Dan Lahan Terhadap Produk Domestik Regional Bruto (Pdrb) Di Sektor Pertanian, Kehutanan Dan Industri : Studi Di Provinsi Lampung." Jurnal Sylva Lestari 5, no. 1 (January 26, 2017): 61. http://dx.doi.org/10.23960/jsl1561-70.

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National economic growth is an aggregate of regional economic growth. Economic growth inboth national and local level is closely related to the performance of the productions of goodsand services, which measured by massive increase in the amount of the Gross Domestic Product(GDP) and Regional Gross Domestic Product (RGDP) for the region. Lampung province’seconomic growth performance is high enough but on the other hand had to be paid by landconversion. This study aims to determine the dynamic of changes in land cover and forest and itsimpact on agriculture, forestry and industrial earnings. Data collected consist of satelitte imageof lampung province RGDP in agricultural sector, RGDP in foresty sector, RGDP in industrialsector and population density data. The result showed that there was a significant relationshipbeetwen changes in private forest cover by 11.055 (p= 0.062), rice field by 7.982 (p= 0.082), andpopulation density by -8.676 (p= 0.000) to the RGDP in agricultural sector. RGDP in theforestry sector is affected significantly by the national forest cover by 1.160 (p= 0.00)and other land use by -0.803 (p= 0.061). RGDP in the industrial sector is influenced significantly byprivate forest -7.434 (p= 0.077), and plantation by 5.471 (p= 0.00).Keyword : RGDB agriculture sector, RGDB forestry sector, RGDB industri sector

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Santi, Nindya Eka, Aisyah Jumiarti, and Fivien Muslihatinningsih. "Analisis Kausalitas Pengeluaran Pemerintah, Investasi, dan Tenaga Kerja Terhadap Produk Domestik Regional Bruto SWP Jember dan Sekitarnya." e-Journal Ekonomi Bisnis dan Akuntansi 5, no. 1 (May 22, 2018): 6. http://dx.doi.org/10.19184/ejeba.v5i1.7707.

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RGDP is the total of all goods and services value which produced by all economics unit within a region. This study aims to determine the causality’s direction between government budget and RGDP, investment and RGDP, labor and RGDP, using panel data on RDU Jember and its regional area during 2000– 2014. The Granger causality test is used to identify the direction of the relationship between the variable between government budget and RGDP, investment and RGDP, labor and RGDP. The result of this study showed that there is a causal relationship between variables.Keywords: Granger causality, RGDP, government budget, investment, and labor

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WEN, JUN, CHUN-PING CHANG, JIA-HSI WENG, and JILIANG LIU. "GLOBALIZATION AND REAL GDP: NEW EVIDENCE USING PANEL VECTOR AUTOREGRESSION." Singapore Economic Review 61, no. 05 (December 2016): 1550065. http://dx.doi.org/10.1142/s0217590815500654.

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The existence and the direction of the relationship between globalization and real GDP (RGDP) are very debatable. By employing annual data of 92 countries from 1970 to 2011, we apply Pedroni’s [Econometric Theory, 20, (2004) 597–625] panel cointegration test and the panel vector autoregressions (VARs) model, proposed by Love and Zicchino [Quarterly Review of Economics and Finance, 46(2), (2006) 190–210], to investigate again the relationship between these two variables. The results first present the weak evidence of cointegration between RGDP and overall globalization and its three main sub-indices. Second, the empirical evidence shows a bidirectional causality between RGDP and overall globalization, economic as well as social, but higher political globalization harms RGDP in the sample countries. Hence, we offer evidence for a clear bidirectional effect between RGDP and each globalization index in Organization for Economic Cooperation and Development (OECD) countries, but not in non-OECD ones. Several empirical implications and suggestions are proposed through our observations.

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Kunwar, Keshar Bahadur. "Foreign direct investment and economic growth of Nepal: An application of bound testing approach to cointegration." Siddhajyoti Interdisciplinary Journal 1 (January 30, 2020): 104–13. http://dx.doi.org/10.3126/sij.v1i0.34925.

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This study employed bounds test based cointegration technique using annual time series data from the period 1990/91 to 2015/16 for exploring relationship between RGDP and FDI in Nepal. This paper examines the effect of FDI on RGDP is insignificant at five percent level of significance. The coefficient (0.35) of (FDI) shows that one percent increase in FDI leads to over 0.35 percent increase RGDP in the long-run. There is no causality between foreign direct investment and economic growth.

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Omodero, Cordelia Onyinyechi. "Effect of Money Supply on Economic Growth: A Comparative Study of Nigeria and Ghana." International Journal of Social Science Studies 7, no. 3 (March 12, 2019): 16. http://dx.doi.org/10.11114/ijsss.v7i3.4137.

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The effect of money supply in enhancing economic growth in Nigeria and Ghana is investigated in this study. The major objectives of the study are to establish the joint and individual influences of money supply mechanisms on economic growth in Nigeria and Ghana. The study employs data from 2009 to 2018 and uses Ordinary Least Squares regression technique for analysis of the data. The findings reveal that broad money supply (M2) has an insignificant negative influence on RGDP in Nigeria, but in Ghana the impact is significant and positive. Broad money supply (M3) exerts insignificant positive influence on RGDP in Nigeria, but significant negative impact on RGDP in Ghana while credit to private sectors (CPS) has insignificant positive influence on RGDP in both Nigeria and Ghana. The study among others suggests that the Monetary Authorities in the two countries should come up with monetary policy strategies that will help drive the economy better and such policies should consider M2 and CPS more as their contributions are necessary for economic expansion that lead to more output and employment.

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Delprat, Laurent, and Ludovic Lebrat. "RGDP et droit à l’oubli." Revue d'Orthopédie Dento-Faciale 53, no. 4 (November 2019): 405–18. http://dx.doi.org/10.1051/odf/2019035.

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Dans une période où la e-réputation et les référencements sur la toile prennent une ampleur exponentielle, la loi CNIL du 20 juin 2018 issue du Règlement général sur la protection des données du 24 mai 2016 amènent enfin une protection notamment des professionnels à posteriori en octroyant un droit à la protection des données personnelles sur internet via l’instauration d’un droit à l’oubli numérique, lequel s’entend d’un droit à l’effacement et d’un droit au déréférencement. Le droit à l’effacement permet de demander à un organisme l’effacement de données à caractère personnel vous concernant, et ainsi de supprimer vos données en ligne. Le droit au déréférencement vous permet de demander à un moteur de recherche de supprimer certains résultats de recherche associés à vos noms et prénoms, sans pour autant effacer l’information sur le site internet source.

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A., Ademuyiwa J., and Adetunji A. A. "Impact of Some Economic Variables on the Real Gross Domestic Product of Nigeria." Budapest International Research and Critics Institute (BIRCI-Journal) : Humanities and Social Sciences 2, no. 4 (November 6, 2019): 12–19. http://dx.doi.org/10.33258/birci.v2i4.563.

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The influences of External Debt Service (EDS), External Debt Stock (EDSt), Government Expenditure (GE), Inflation Rate (InfR), Interest Rate (IntR) and Exchange Rate (ExR) of Nigeria on the Real Gross Domestic Product (RGDP) are examined. Results of the analysis using Stepwise Regression (Backward Elimination and Forward Selection) reveals that GE, EDS, and IntR have positive significant contributions to the RGDP of the country compared to other variables considered.

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Anthony Olugbenga Adaramola and Modupe F. Popoola. "Long and Short Run Relationship between Stock Market Development and Economic Growth in Nigeria." Journal of Economics and Behavioral Studies 11, no. 5(J) (December 9, 2019): 45–53. http://dx.doi.org/10.22610/jebs.v11i5(j).2965.

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We examined the long and short run association subsisting between stock market development(market capitalisation, value of transactions, number of deal and all share index), and Nigerian economicgrowth (RGDP) with quarterly data from 1986 to 2017. The Autoregressive Distributed Lag (ARDL) model isapplied for the purpose of estimation. The ARDL bound test result revealed that all the indicators of marketdevelopment exert positive effect on the RGDP in the short run. Further, all the indicators except number ofdeals, have direct and significant relationship with economic growth. Moreover, we find that marketdevelopment causes economic growth. Consequently, we recommend a need for the implementation ofpolicies and procedures capable of enhancing investors’ confidence and boosting market because of theirperceived multiplier impacts on economic growth. Effort should also be focused on the enhancement of stockmarket size which in turn will provide the needed fund for investment and thus resulting in rise in the RGDP.

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Kresnanto, Nindyo Cahyo. "Model Pertumbuhan Sepeda Motor Berdasarkan Produk Dosmetik Regional Bruto (PRDB) Perkapita (Studi Kasus Pulau Jawa)." MEDIA KOMUNIKASI TEKNIK SIPIL 25, no. 1 (August 10, 2019): 107. http://dx.doi.org/10.14710/mkts.v25i1.18585.

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The number and growth of vehicles, is a separate issue associated with sustainable transportation. In 2014, recorded vehicle election in Indonesia reached more than 448 vehicles per 1,000 people. And specifically, for motorcycles reach 365 vehicles per 1,000 people. The growth of motor vehicles is significantly influenced by economic growth (measured by Gross Domestic Product - RGDP). When compared with motor vehicle growth, it can be concluded that the high growth rate of motor vehicles at the end of this decade actually impact on the decrease of RGDP. To see the trend between economic growth represented by RGDP and the growth of motor vehicle (motorcycle) needs a model. Motor vehicle growth model in Java can be approached with Gompertz function. This function is a negative exponential function with asymptote used is the highest value of motor vehicle ownership in DKI Jakarta Province is 1,299 motorcycles per 1,000 people.

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Omodero, Cordelia Onyinyechi, and Kabiru Isa Dandago. "Investment in Agriculture and Extractive Industry: A Panacea for National Development." Research in World Economy 11, no. 1 (March 6, 2020): 34. http://dx.doi.org/10.5430/rwe.v11n1p34.

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Economic diversification into agriculture and extractive industry in Nigeria has been a fascinating and crucial economic issue that deserves consideration especially as the country is shifting from mono-economy (caused by oil boom) to other viable economic sectors. The global economic meltdown and depression have stimulated countries to look into other sectors of the economy in order to enhance their national development. Hence, this study tries to examine the contribution of agriculture and extractive industry to the Nigeria’s real gross domestic product (RGDP). The study makes use of time series data gathered from CBN Statistical Bulletin ranging from 1981-2017 and employs Ordinary Least Squares (OLS) method as the statistical tool with the aid of e-views version 9. The findings reveal that agriculture has a robust and noteworthy positive impact on RGDP while the solid mineral equally has a substantial positive influence on RGDP. However, crude petroleum (proxy for crude petroleum & natural gas) has a positive inconsequential effect on RGDP. This brings the study to a conclusion that investment in agriculture and solid minerals is highly imperative at the moment. Therefore, the study has suggested that economic diversification should be focused more on agriculture and solid mineral extraction. In addition, the government should try to manage the crude petroleum and natural gas exploration so as to prevent fund repatriation and transfer to other countries due to borrowed technology.

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Dissertations / Theses on the topic "RGDP"

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Thumshirn, Georgette. "Multivalente zyklische RGD-Peptide und RGD-Mimetika für den Einsatz in Tumordiagnostik und Tumortherapie." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=96988950X.

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Engrand, Philippe. "Calix[4]arènes fonctionnels pour ancrage sur polymère naturel et antennisation par le tripeptide RGD préparé par synthèse convergente." Thesis, Nancy 1, 2008. http://www.theses.fr/2008NAN10143/document.

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Ce travail porte sur la valorisation du calix[4]arène comme complexant de métaux de transition, immobilisé sur un matériau, et comme vecteur de principe actifs, aptes à interagir avec des récepteurs cellulaires. En premier lieu, la monofonctionnalisation sélective d’un calix[4]arène, sur sa partie haute ou sur sa partie basse est réalisée. Les fonctions nitro, amine, adéhyde, et alcène ont été incorporées sur la partie basse, via un bras espaceur, et un bras acide a été incorporé sur la partie haute. Ces calix[4]arènes monofonctionnalisés se déclinent en trois séries de composés, dépendant du degré et de la nature des substituants sur la partie basse : tétrol, triméthoxy et trisbipyridyle. Le second objectif est le greffage d’un calix[4]arène pourvu d’une fonction amine, sur un polymère naturel, cellulose ou dextran. Un mode opératoire reproductible a été mis au point, qui a permis d’obtenir un calix[4]arène complexant de l’ion Cu(I), immobilisé sur cellulose en poudre ou sur dextran. L'évaluation du taux de greffage par RMN et dosage UV-Vis en présence de Cu+ est décrite pour les dextrans. Enfin, nous avons incorporé sur la partie haute du calix[4]arène d'antennes de reconnaissance cellulaire de type RGD, préparées par synthèse convergente en solution. Une chimiothèque de tripeptides RGD, modulés sur les parties C- et N-terminales, a été obtenue avec de bons rendements. Ces peptides ont fait l’objet d’une évaluation biologique (adhésion cellulaire). Nous avons ainsi préparé des calix[4]arènes, de conformation conique, antennisés par le dipeptide GD et le tripeptide RGD, destinés à élaborer des modèles de vecteurs supramoléculaire de principes actifs
The aim of this present work is dedicated to the valorization of calix[4]arene as a transition-metal complexing agent, grafted on a polymeric material, and as a drug carrier, displaying a cell recognition peptidic sequence. We describe selective monofunctionalization of a calix[4]arene, at the upper rim or at the lower rim. Nitro, amino, aldehyde and alcene functions were incorporated on the calix[4]arene lower rim, via a spacer. In the same way, an acid arm was also incorporated on the calix[4]arne upper rim. We made those monofunctionalized calix[4]arenes available into three series, depending on the number and the nature of substituent on the three residual OH groups of the calix[4]arne carried function : tetrol, trimethoxy and trisbipyridyl. The second goal is dedicated to the grafting of an amino-fonctionalized calix[4]arene, on a natural polymer, such as cellulose or dextran. We have developped a method allowing to access to a new polymeric complexing agent. We also describe the grafting-ratio, calculated by NMR, UV-Vis and titration with Cu+. The third goal is dedicated to the grafting of the cell-recognizing RGD sequence, prepared in solution by a gram-scale convergent synthesis. A RGD analogue library, functionalized by variable groups on the C- and N- terminations, were obtained with interestly yields. Those peptides were involved into a biological evaluation (cellular adhesion). Thus, cone-conformed calix[4]arene incorporating the GD or RGD peptide sequence were elaborated as preliminary models for the access to new drug-carrier molecular devices

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Lee, Ted. "Triggerable ligand presentation using caged-RGD." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/52943.

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Cells rely on time-dependent binding and activation by the ECM to initiate downstream signal transduction. It is unknown whether adhesion to a ligand is required throughout various cell processes, or only during a specified time period ("temporal threshold”). Current approaches to ligand presentation often comprise of static, constant densities of ligands. In contrast, natural cell adhesive interactions with ECMs exhibit spatiotemporal patterns of binding and activation. Therefore, a key to future research in controlling cell-material interactions will be the development of materials that can respond to external stimuli. The objective of this project is to engineer biomaterials that present a UV-labile caged-Arginine-Glycine-Aspartic Acid (RGD) ligand and evaluate the effects on cell activities. RGD is the minimal adhesive sequence of fibronectin. By dynamically modulating adhesive ligand presentation, the effects of temporal control on cell processes can be elucidated. In this caged-peptide, a photo-labile group adjacent to the aspartic acid residue of RGD effectively “masks” a cyclo(RGDfk) peptide. Upon UV irradiation (360 nm), the caging group is released thereby restoring the adhesive activity of the peptide. By having unparalleled spatiotemporal control of RGD ligand presentation, we demonstrated two novel tools for discovery: 1) in vivo ligand presentation to probe downstream tissue behavior and cell infiltration to biomaterial implants, and 2) in vitro ligand presentation in situ using confocal-based live cell microscopy to investigate real-time vinculin recruitment and cell traction force generation. These studies represented the first demonstration of triggerable adhesive ligand presentation in vivo and demonstrated the utility of caged-compounds for probing specific receptor-ligand responses on highly defined PEG-based hydrogels. Triggerable in vitro ligand presentation, combined with traction force microscopy, demonstrated a new research tool for investigating focal adhesion formation and downstream force generation. Taken in whole, these results provide previously unknown insights into the importance of spatiotemporal control of adhesive ligands and created novel new research platforms for future discovery.

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McDonagh, Birgitte Hjelmeland. "Optimalised Carbodiimide Chemistry for RGD-coupled Alginate." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-16807.

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Alginate is a naturally ocurring polyanion of (1→4)linked β-d-mannuronic acid (M)and its C-5 epimer α-l guluronic acid (G). The polyanion, and particularly longstretches of guluronic acids, chelates and form hydrogels in the presence of divalentcations such as Ca2+ . Chelation occur at physiological conditions and the formed hy-drogels are biocompatible and stable. These properties nominates alginate hydrogelsas promising biomaterials in tissue engineering applications. Encapsulation of cells inalginate beads is easily prepared by mixing cells with high molecular weight alginate,and dripping the solution into a CaCl2 solution. When alginate comes into contactwith Ca2+ ions, alginate beads are immediately formed and the cells are captured ina three dimensional alginate matrix. Once inside the alginate capsule, the cells canbe transplanted into a host deﬁcient in the particular cells. The alginate protectsthe cells against the immune system of the host, opening for allograf transplantationwithout immunosuppressiva. The pores in the alginate network are big enough toenable diﬀusion of waste and nutrition through the membrane, which is importantfor cell survival.Alginate is in itself not cell adhesive. However, alginate can be tailored into a celladhesive biomaterial by attachement of cell adhesive peptides, such as the RGDmotif found in extracellular matrix molecules such as ﬁbronectin. Coupling of celladhesive peptides increase cell survival in three dimensional alginate matrices. Inthis study, RGD-alginate is tailored with a chemoenzymatic approach that ensuresRGD-coupling to non-gelling residues. This procedure starts with non-gelling man-nuronan that is chemically modiﬁed by attachement of the cell adhesive peptideGRGDYP by carbodiimide chemistry. Gelling residues are introduced to peptidecoupled mannuronan by a two step epimerisation catalyzed by the epimerases AlgE4and AlgE6.In this study, the carbodiimide chemsitry used for coupling GRGDYP to mannuro-nan is optimalised to create alginate with more than 0.2% bound peptide. Themodel molecules ﬂuoresceinamine, 4-aminophenol and L-tyrosine-methyl ester areused for optimalisation, and the latter model molecule was found to give the bestrepresentation of peptide coupling to mannuronan. The carbodiimide mediated cou-pling to mannuronan is investigated by varying the pH, temperature and reactantconcentrations. The degrees of coupling for each intervention is assessed by 1 H NMRand UV/vis spectroscopy. The presence of covalently bound by-products, namedN-acylurea adducts, to mannuronan is assessed by 1 H NMR spectroscopy and acontrolled reduction of these unwanted compounds is attempted. The results from the optimalisation indicates that coupling of Me-O-Tyr and GRGDYP to mannuro-nan is concentration-dependent, as an increase in coupling was observed when theGRGDYP and Me-O-Tyr concentrations was increased. The highest peptide incor-poration described in this study was 3.4% GRGDYP coupled to mannuronan, whichis higher compared to similar studies.The optimalised carbodiimide chemistry is applied to a large scale batch of RGD-coupled alginate that is to be used for cell encapsulation of olfactory ensheating cellsfrom neonatal rat brain.High molecular weight alginate was coupled with 0.45% GRGDYP and ﬁltratedwith active coal before cell encapsulation. The coal ﬁltration removed a substantialamount of the unwanted N-acylurea adducts but also removed peptides, resultingin a GRGDYP coupling of 0.1% calculated from UV/vis spectroscopy, and 0.4%calculated from 1 H NMR spectroscopy. This indicates that peptides are associatedwith N-acylurea adducts and that active coal ﬁltration is necessary to remove them.Encapsulation of olfactory ensheating cells to RGD-coupled alginate gave no mor-phology changes or enhanced cell viability compared to non-peptide coupled alginate.It is believed that the low enzymatic action of the AlgE6 epimerase, a low concen-tration of peptides or a combination of both, have inﬂuenced the cell viability andlack of morphology changes. Enhanced peptide incorporation can be achieved byincreasing the reactant concentrations of the peptides, but this would lead to a moreexpensive procedure. Increased eﬃcacy, meaning a higher peptide coupling withlower adduct formation, at lower peptide concentrations was not achieved. However,the use of sodium borohydride in combination with periodate oxidised alginates forpeptide coupling should be assessed as a novel approach for increased peptide yieldsat lower peptide concentrations.

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Halie, Delphine. "Synthèse diastéréosélective de mimes du peptide RGD." Paris 5, 2006. http://www.theses.fr/2006PA05P639.

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Cressman, Sonya. "RGD-containing ligands for targeting liposomal nanoparticles." Thesis, University of British Columbia, 2007. http://hdl.handle.net/2429/31279.

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The use of a targeting ligand to enhance the delivery of liposomal nanoparticles (LNs) to specific cells in diseased tissue is an attractive strategy that has progressed very slowly since the idea originated over 25 years ago. The slow progress can be attributed, in part, to the difficulties involved in producing well-defined targeted LN systems. This work concerns the development of peptide-based ligands for targeting LNs to cells that overexpress the α[sub v]β₃ integrin, which is considered a unique marker of the tumor-associated endothelium. Initial work focused on using Fab' fragments of monoclonal antibodies as targeting ligands and employing literature techniques for coupling these proteins to lipids that can then be inserted in preformed LNs. However, it was found that the coupling procedures resulted in low yields of poorly defined lipid-ligand conjugates that were difficult to quantitate when incorporated into LN. Attention was then given to the development of a peptide-targeting agent that could be incorporated into an LN at the time that the LN is made. The RGD-containing cyclic peptide, eRGDfK, was employed as the targeting ligand. In order to characterize its binding to the α[sub v]β₃ integrin, a fluorescently labeled analogue, cRGDfK-488, was synthesized and capillary electrophoresis was employed for analysis. This procedure proved advantageous for studying receptor ligand interactions, since it allowed for the binding to be characterized in solution without the need for covalent modification of receptor or ligand. A 2:1 RGD ligand to integrin specific binding stoichiometry was revealed with the second binding event having a similar affinity as the first binding event. The next phase of these studies investigated the ability of cRGDfK-488 to bind to integrins on human umbilical vascular endothelial cells (HUVEC) and subsequently undergo endocytosis. This was compared to the binding and uptake properties of a fluorescently labeled monoclonal antibody, LM609X, which specifically binds α[sub v]β₃ integrin. Using flow cytometry and fluorescence microscopy, it is shown that the RGD ligand exhibited considerably greater uptake following incubation at endocytosis permitting temperatures (37°C) as compared to endocytosis inhibiting temperatures (4°C). A 7.4-fold increase in uptake of the RGD-lipid was observed following a one-hour incubation with HUVEC at 37°C, as compared to 4°C. In contrast, only a 1.9 fold increase in cell-associated fluorescence was observed on incubation with LM609X at 37°C as compared to 4°C. It is suggested that this ability of RGD ligands to stimulate endocytosis may be of utility for achieving enhanced intracellular delivery of ligand-associated drugs in anti-angiogenic applications. The cyclic peptide was then used to construct a fluorescently labeled RGD-spacer-lipid construct of defined molecular weight that could be incorporated into LN at the time of manufacture. It is shown that the resulting RGD-LNs bind to HUVEC with increasing avidity as the amount of RGD-spacer-lipid incorporated is increased. Further, these RGD-LNs are straightforward to make and can load and retain anti-cancer drugs such as doxorubicin. It is shown that RGD-LNs loaded with doxorubicin and incubated with HUVEC selectively deliver the drug to the cytosol while non-targeted LNs are not internalized by the cell, suggesting potential utility as targeted drug delivery systems in vivo.
Medicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate

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Zairi, Amel. "Elaboration et Caractérisation de revêtements à base de nitrure de chrome par pulvérisation cathodique magnétron en condition réactive : Propriétés mécaniques et tribologiques." Phd thesis, Ecole nationale supérieure d'arts et métiers - ENSAM, 2013. http://pastel.archives-ouvertes.fr/pastel-00996656.

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Élaborer et optimiser des nouveaux matériaux restent toujours parmi les enjeux liés à l'amélioration de la performance de la durabilité des matériaux.Le domaine des traitements de surface et plus particulièrement les nanotechnologies utilisées pour produire des revêtements en couches minces plus précisément les dépôts physiques en phase vapeur (PVD), permet de synthétiser des nouveaux matériaux qui peuvent résister dans des conditions sévères de diverses applications.L'industrie des outils de coupe et plus précisément le bois dans notre cas, nécessitent de plus en plus la synthèse des nouveaux matériaux et nanomatériaux pour améliorer la tenue en service des outils de coupe.L'objectif de ce présent travail est de développer et caractériser des revêtements dont le matériau de base est le chrome et/ou le silicium en vue de les optimiser. L'élaboration a été réalisée par différentes méthodes à savoir la pulvérisation cathodique magnétron radio fréquence ou encore en mode courant direct. En outre, la RGPP (reactive gas pulsing process) a été exploité pour la première fois dans ce travail dont le but est de surmonter les difficultés rencontrées avec la pulvérisation réactive.Les différentes caractéristiques mécaniques et tribologiques des couches ont été étudiées et corrélées avec les caractéristiques physicochimiques et microstructurales.Il s'avère que la pulvérisation cathodique en mode courant direct prouve son importance par l'obtention des couches de meilleure qualité que celles obtenues en mode radio fréquence. Pour des contenus d'azote similaires on obtient des propriétés mécaniques et tribologiques plus importantes. On note que les propriétés mécaniques réalisent un gain de 60 % par rapport à celles obtenues en mode RF. En outre l'ajustement des paramètres de la méthode RGPP (le rapport cyclique et la période des pulses) influe considérablement les caractéristiques des couches obtenues et on montre une amélioration de la performance des couches. A l'issue de l'injection pulsée du gaz réactif, on montre que l'alternance entre l'ouverture et la coupure de la vanne du gaz réactif est indispensable pour la déposition dans un milieu réactif afin d'éliminer les composés métalliques sur les cibles obtenus lors de dépôt.Enfin, l'ajout de silicium au CrN mène à obtenir des solutions solides ou une structure nanocomposite selon la teneur de silicium dans la couche ce qui implique une amélioration des caractéristiques mécaniques et par la suite celles tribologiques.L'apport de ce travail de thèse est de présenter une étude globale sur la performance des couches CrN et CrSiN en corrélant ses caractéristiques mécaniques et tribologiques avec les paramètres physiques de dépôt et en adoptant plusieurs méthodes d'élaboration.

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Gibson, Christoph. "Kombinatorische Festphasensynthese und biologische Evaluation niedermolekularer RGD-Mimetika." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=959981314.

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Moncelet, Damien. "Propriétés d'agent de ciblage et de molécules cytotoxiques pour l'IRM et la thérapie de gliomes." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0166/document.

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L'objectif de cette thèse concerne la possibilité d'améliorer le diagnostic et la thérapie des gliomes par le ciblage des intégrines à l’aide du RGD et par le développement d'agents multimodaux de type alcoxyamine. L’étude de l’internalisation du RGD révèle une régulation par la densité cellulaire, paramètre histologique dans la catégorisation des gliomes. Dans notre modèle, la densité cellulaire impacte la contribution de l’endocytose clathrine-dépendante et le métabolisme mais n’influence pas le rôle du cytosquelette. La régulation de l’internalisation des peptides RGD par la densité cellulaire reste à mieux comprendre afin de perfectionner les agents utilisant ce ciblage pour l’imagerie et le diagnostic des gliomes. Dans le même temps, les propriétés multimodales des alcoxyamines ont été évaluées àdes fins théranostiques. Ces molécules s’homolysent spontanément pour libérer un nitroxyde et un radical alkylant cytotoxique pouvant en plus induire une réactivation immunitaire antitumorale. Le nitroxyde est un agent de contraste pour l’IRM rehaussée par effet Overhauser. Le fort rehaussement du signal observé à proximité du nitroxyde assure un suivi en temps réel de l’apparition de l’agent alkylant. L’adaptation des alcoxyamines pour une homolyse conditionnelle dans le gliome permettrait une action thérapeutique avec un contrôle spatial et un suivi temporel du composé cytotoxique. L’acheminement de molécules d’intérêt vers la cible est rendu difficile par la présence de barrières physiologiques. Dans ce travail, la progression de nanoparticules par la voie intratrachéale peut se substituer à celle intraveineuse avec une augmentation du temps de rétention dans le gliome
The aim of this thesis is to improve the diagnostic and the therapy of glioma through both the integrin targeting by RGD and the development of Alkoxyamine as multimodal agent. The RGD internalization is regulated by the cellular density, a histologic parameterfor the glioma classification. In our model, the cellular density increases the contribution of both the clathrin-mediated endocytosis and the metabolism but not the one of the cytoskeletal. A better knowledge about the RGD internalization regulation by the cell density could help the MRI probe development for glioma diagnosis. Properties of alkoxyamine as multimodal agent were evaluated to perform theranostic. The spontaneous alkoxyamine homolysis give a nitroxide radical and a cytotoxic alkylating agent that could induce immune reactivation against the tumor. This nitroxide is an Overhauser enhanced MRI contrast agent. The strong signal enhancement in the nitroxide vicinity gives information in real-time about the release of the alkyl radical. Alkoxyamine adaptation for a conditional homolysis through specific glioma proteolysis activity could induce a localized alkyl therapeutic effect with a real-time monitoring. Physiological barriers limit the drug accumulation in the targeted sites. In this study, the intratracheal instillation of nanoparticles can substitute the intravenous administrationincreasing their intratumoral retention time

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Lu, Xinjie. "Structure and function studies of the RGD protein dendroaspin." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309313.

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Books on the topic "RGDP"

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Jean-Claude, Nemery, Université de Reims Champagne-Ardenne. Centre de recherche sur la décentralisation territoriale, and Groupement de recherches sur l'administration locale en Europe, eds. RGPP et réforme des collectivités territoriales. Paris: L'Harmattan, 2012.

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Separat︠s︡ii︠a︡ i individuat︠s︡ii︠a︡ lichnostʹ i semʹ︠i︡a: Materialy VIII i IX konferent︠s︡iĭ MAAP, ROAP i RGAP SPb. Sankt-Peterburg: Skifii︠a︡ print, 2010.

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International, Symposium on Rarefied Gas Dynamics (24th 2004 Bari Italy). Rarefied gas dynamics: 24th International Symposium on Rarefied Gas Dynamics, Monopoli (Bari), Italy, 10-16 July 2004 : RGD 24. Melville, New York: American Institute of Physics, 2005.

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House, Happy. Hh-Rgdy Ann's Pnt Bx. Random House Books for Young Readers, 1988.

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House, Happy. Hh-Rgdy Ann&andy's#fun. Random House Books for Young Readers, 1987.

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House, Happy. Hh-Rgdy Ann&andy Plyrm. Random House Books for Young Readers, 1987.

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Wingate, Maria. Playing electronic keyboard for beginners (Electronic keyboard series). Sight & Sound, 1988.

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House, Happy. Hh-Rgdy Ann Bk Go-to. Random House Books for Young Readers, 1988.

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House, Happy. Hh-Rgdy Ann&andy S COL. Random House Books for Young Readers, 1987.

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House, Happy. Hh-Rgdy Ann in Magic B. Random House Books for Young Readers, 1987.

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Book chapters on the topic "RGDP"

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Zhao, Ming, Shi-qi Peng, Meng-shen Cai, Chao-shu Tang, and Chang-ling Li. "Antiplatelet and vasodilation effects of RGDS." In Peptides, 176–78. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-010-9066-7_52.

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Pons, Jean-François, Mamadou Sow, Frédéric Lamaty, Jean-Luc Fauchére, Annie Molla, Philippe Viallefont, and René Lazaro. "New RGD amphiphilic cyclic peptide and new RGD-mimetic constrained diketopiperazines." In Peptides Frontiers of Peptide Science, 176–77. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-46862-x_69.

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Liu, Shihao, Quan Chen, Zihan Yue, and Jie Ma. "Complex Water Surface Segmentation with RGBP-FCN." In Intelligent Robotics and Applications, 367–76. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-97589-4_31.

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Kang, Keon Wook, Jae Min Jeong, and Ambros J. Beer. "Angiogenesis PET Using Radiolabeled RGD Peptides." In PET-CT Beyond FDG A Quick Guide to Image Interpretation, 195–211. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-93909-2_12.

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Klein, Scott I., Bruce F. Molino, Valeria Chu, Zaverio Ruggeri, Mark Czekaj, Charles J. Gardner, Jack Newman, and John A. Barrett. "Novel RGD analogs as antithrombotic agents." In Peptides 1990, 374–75. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3034-9_157.

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Pfaff, Martin. "Recognition Sites of RGD-Dependent Integrins." In Integrin-Ligand Interaction, 101–21. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4757-4064-6_4.

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Tjoeng, F. Siong, Kam F. Fok, Mark E. Zupec, Robert B. Garland, Masateru Miyano, Susan Panzer-Knodle, Lucy W. King, et al. "Peptide mimetics of the RGD sequence." In Peptides, 752–54. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2264-1_302.

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Saraf, Poonam, Xiaoling Li, and Bhaskara Jasti. "Integrin Targeting Using RGD-Based Peptide Amphiphiles." In Methods in Pharmacology and Toxicology, 135–55. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/7653_2015_61.

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Kolb, Hartmuth C., Fanrong Mu, Umesh Gangadharmath, Vani P. Mocharla, Zhihong Zhu, Ashok Chaudhary, and Joseph C. Walsh. "Radiosynthesis of [18F]Flotegatide ([18F]RGD-K5)." In Radiochemical Syntheses, 29–40. Hoboken, NJ: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781118834114.ch4.

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Rechichi, A., S. Sartori, A. Caporale, G. Ciardelli, G. Vozzi, L. Mazzucco, E. Peggion, and P. Giusti. "Development of a RGDS-peptide modified polyurethane for tissue regeneration." In Advances in Experimental Medicine and Biology, 249–50. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-73657-0_114.

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Conference papers on the topic "RGDP"

1

Charon, M. H., L. Tranqui, A. Andrieux, G. Hudry-Clergeon, and G. Marguerie. "FIBRINOGEN BINDING TO ENDOTHELIAL CELLS AND INTERFERING PEPTIDES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644735.

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Fibrinogen interacts with platelets and endothelial cells via specific binding sites. While the platelet fibrinogen receptor has been identified and was found to be associated with GPIIb-IIIa, the binding site on endothelial cells has not been characterized yet. The platelet GPIIb-IIIa belongs to the newly identified cytoadhesin family which includes immunologicaly related receptors interacting with RGD containing proteins. A cytoadhesin has recently been described on endothelial cells and the possibility that fibrinogen might interact with this glycoprotein was examined. Peptides corresponding to γ and α chains sequences were synthesized and their capacity to inhibit fibrinogen binding to endothelial cells and platelets were compared. Analogues of the γ chain from His 400 to Val 411 produced an inhibition similar to that observed for fibrinogen platelet interaction, suggesting that the structure function relationship of γ peptides is identical in both systems. In contrast synthetic analogues corresponding to the a chain and including RGD yielded slightly different results. While RGD alone was inactive on platelet, this tripeptide was active on endothelial cells. RGDS and RGDF corresponding to α 572-575 and α 95-98 partially or fully inhibited fibrinogen binding to endothelial cells but the structure activity relationship was different when compared to that observed for platelets. Addition of the N and C sequences adjacent to RGDS reduced the activity of this peptide whereas the activity of RGDF analogues was not modified by addition of N and C-sequences. In contrast platelet fibrinogen binding decreased with these RGDF analogues. These results suggest that fibrinogen endothelial cell binding is mediated by an RGD adhesion receptor with subtle differences in the recognition selectivity of the ligand, which is controled by the surrounding sequence.

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Parise, L. V., B. Steiner, L. Nannizzi, and D. A. Phillips. "PEPTIDES FROM FIBRINOGENAND FIBRONECTIN CHANGE THE CONFORMATIONOF PURIFIED PLATELET GLYCOPROTEIN IIb-IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643697.

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Specific amino acid sequences in fibrinogen and fibronectin appear to mediate the binding of these ligands to the glycoprotein (GP) IIb-IIIacomplex in platelets. Thesesequences include LGGAKQAGDV from the y chain of fibrinogen, and RGD(S) from the a chain of fibrinogenand the cell-binding domain of fibronectin. Several recent reports suggest thatfibrinogen and/or peptides with these sequences cause clustering of GPIIb-IIIa on the platelet surface and Na+/H+ exchange in epinephrine-stimulated platelets. Thus, it is possible that occupancy of specific sites on GP Ilb-IIIa affects its conformation, initiating such events. In this study,we determined whether LGGAKQAGDV, RGDS, and related peptides affect the conformation of purified platelet GP IIb-IIIa. Conformational changes in GP IIb-IIIa were evaluated bychanges in proteolytic susceptibility and hydrodynamic properties. Thepurified GP IIb-IIIa complex was fund to be resistant to proteolysis bythrombin. However, pretreatment of GP IIb-IIIa with various peptidesincreased the susceptibility ofGP libα to thrombin-induced proteolysis,as quantitated onpolyacryfamide gels.The order of potency of these peptides was RGDS<LGGAKQAGDV < KGDS < RGES. This order of potency agrees with that for the abilityof these peptides to inhibit 125I-fibrinogen binding to platelets. The effect of the peptides on proteolysis was time-, temperature-, and concentration-dependent; RGDS Induced a half-maximal effect at ˜60μM. Evaluation of the hydrodynamic properties of GP IIb-IIIa showed that LGGAKQAGDV orRGDS, but not RGES, decreased thesedimentation coefficient of GP IIb-IIIa from 8.5S to 7.7 S or7.4, S,respectively. This changewas accompanied by an increase in theStoke’s radius from 73 A to 84 A. These results suggestthat LGGAKQAGDV andRGDS alterthe conformationof the purified GPIIb-IIIa heterodimer complex by causing it to unfold.This change in conformation may be related to changesin the distribution and function of GP IIb-IIIaon the platelet surface that occurwith occupancy ofligand binding sites.

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Taki, Y., Y. Morita, S. Nishimura, A. Hirata, T. Koga, and E. Nakamachi. "Development of Surface Treatment Technique With Photolytic Macromolecule Including RGDS Peptide." In ASME 2016 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/imece2016-66466.

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Arg-Gly-Asp-Ser (RGDS) coating is one of effective methods to improve the cell adhesive property of the scaffold surface. However, it is difficult to regulate the RGDS quantity and distribution, and to visualize RGDS distribution. The purpose of this study was to develop a surface treatment technique that the RGDS quantity can be regulated with the ultraviolet rays irradiation and the RGDS distribution can be visualized with the fluorescence. P(MMA-g-ANP-RGDS) and P(HEMA-g-ANP-RGDS) were respectively synthesized by radical copolymerization of methylmethacrylate (MMA) and 2-hydroxyethylmethacrylate (HEMA) with peptide-macromonomer containing photo-labile linker (3-amino-3-(2-nitrophenyl)propionic acid (ANP)) and RGDS. Each polymer film was produced by using spin-coater, and then ultraviolet rays was irradiated to the each film through the glass mask with three different ultraviolet rays transmissivity of 0 %, 30 % and 60 %. In both polymer films, the RGDS quantity can be regulated by ultraviolet rays irradiation, and the luminance decreased same as the RGDS quantity. Adherent osteoblast-like cells were not observed on P(HEMA-g-ANP-RGDS) film, but the number of adherent osteoblast-like cells was increased with increasing the RGDS quantity on the P(MMA-g-ANP-RGDS) film. In conclusion, we accomplished to develop the surface treatment technique with P(MMA-g-ANP-RGDS) to regulate and visualize the RGDS quantity and distribution.

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Flasch, Oliver, Olaf Mersmann, and Thomas Bartz-Beielstein. "RGP." In the 12th annual conference comp. New York, New York, USA: ACM Press, 2010. http://dx.doi.org/10.1145/1830761.1830867.

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Nievelstein, P. F. E. M., M. Ottenhof-Rovers, M. D. Pierschbacher, and J. J. Sixma. "THE ARG-GLY-ASP(SER) SEQUENCE OF FIBRONECTIN, AND THE GLYCOPROTEIN IIB-IIIA COMPLEX ARE NOT INVOLVED IN FIBRONECTIN DEPENDENT PLATELET ADHESION IN FLOW." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643590.

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Activated blood platelets interact with fibronectin through it to the glycoprotein IIb-IIIa(GPIIb-IIIa)-complex. The cell attachment site of fibronectin with its crucial arg-gly-asp-(-ser) (RGD(S))sequence is involved in this binding. We have studied the importance of this interaction for the fibronectin dependence of platelet adhesion under flow conditions. An RGDS-containing hexapeptide (GRGDSP) was compared with a non-reactive control peptide (GRGESP). The GRGDSP-peptide inhibited thrombin induced aggregation and adhesion under static conditions at 0.1 mM. This concentration had no effect on platelet adhesion to nonfibrillar collagen type I in flow. GRGDSP at 1 mM had a significant inhibitory effect at 1500 s™1 (8.8 ± 1.4 111In platelets* 105 /cm2, versus 19.8 ± 0.5 for the control). At lower shear rates of 800 and 300 s™1 , where platelet adhesion is also fibronectin dependent, no significant differences were obtained (respectively 11.7 ± 1.1 versus 15.2 ± 2.1, and 11.4 ± 1.0 versus 13.1 ± 0.7).The relation between GPIIb-IIIa and fibronectin dependence was investigated with platelets of a patient with Glanzmann’s thrombasthenia and monoclonal antibodies to GPIIb-IIIa, using endothelial cell matrix (ECM) as a surface. Platelets of normal controls or a patient with Glanzmann’s thrombasthenia showed a inhibition of adhesion in fibronectin free plasma, after the ECM had been preincubated with anti-fibronectin F(ab’)2, of respectively _J5 and 30 percent at 300 s™1 , and 43 and 65 percent at 1300 s™1 . Incubation of platelets with anti GPIIb-IIIa showed inhibition of platelet adhesion at high shear rates. Dependence on fibronectin for platelet adhesion was still observed, even though separate experiments had shown that these anti GPIIb-IIIa antibodies could block binding of radiolabeled fibronectin to thrombin activated platelets. These data suggest the existence of a second binding system from the RGD/GPIIb-IIIa system separate for the interaction of platelets with fibronectin, which may only function when fibronectin is present on a surface.

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Harfenist, E. J., M. A. Packham, and J. F. Mustard. "EFFECTS OF CELL ADHESION PEPTIDE, Arg-Gly-Asp-Ser (RGDS), ON RESPONSES OF WASHED PLATELETS FROM HUMANS, RABBITS AND RATS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643592.

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Fibrinogen (Fbg) is a cofactor in the aggregation of human platelets, and washed platelets do not aggregate to a significant extent in response to ADP unless Fbg is added to the suspension; however, exogenous Fbg is not required for ADP-induced aggregation of washed platelets from rabbits or rats. Since, with human platelets, Arg-Gly-Asp-Ser (RGDS) inhibits aggregation and the binding of 125I-Fbg to ADP-stimulated platelets, its effects on the responses of rabbit and rat platelets were studied in an attempt to elucidate the differences in Fbg requirements of platelets from the three species. Aggregation and Fbg binding were studied using washed platelets suspended in Tyrode solution containing albumin, apyrase and 2 mM Ca2+. 50 μM RGDS caused over 80% inhibition of the aggregation of human platelets stimulated with 9 yM ADP in the presence of 0.2 yM Fbg, but only 3-9% inhibition of the ADP-induced aggregation of rabbit or rat platelets regardless of whether exogenous Fbg was added. 50 yM RGDS also inhibited the aggregation of human platelets stimulated with thrombin (0.9 U/mL), but produced no more than 3% inhibition with rabbit or rat platelets. The binding of 125I-Fbg to ADP-stimulated human platelets was inhibited by 80-90% by 30 yM RGDS, but even at 50 μM, RGDS inhibited Fbg binding to rabbit or rat platelets by only 15-27%. The differences were due to the species of platelets, since, with both human and rabbit platelets, human Fbg could be replaced by rabbit Fbg without significantly changing the results. RGDS, added to human platelets that had been aggregated with thrombin, did not cause deaggregation, but did partially inhibit aggregation when added within 1 min; this inhibitory effect was less than when RGDS was added before thrombin, and decreased progressively as the length of time before the addition of RGDS was increased. These observations indicate a difference in aggregation mechanism between human platelets and those from rabbits and rats, and are consistent with a Fbg-independent component to the aggregation of rabbit and rat platelets.

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Cohen, I., and J. G. White. "DIFFERENT SITES FOR FIBRINOGEN AND FIBRIN RECEPTORS ON PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643521.

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Platelet stickiness and aggregation depend on availability of surface membrane glycoprotein Ilb-IIIa complex to bind fibrinogen. The development of isometric tension in platelet-rich clots is a manifestation of fibrin binding to the cells as well as platelet contractile activity. The possibility that fibrinogen and fibrin may bind to different portions of the GPIIb-IIIa complex has apparently not been considered. In order to determine whether the receptors for the fibrinogen and fibrin on the GPIIb-IIIa complex are identical, we investigated the effect of various monoclonal antibodies to the Ilb-IIIa complex and the tetrapeptide Arg-Gly-Asp-Ser (RGDS), a recognition site on fibrinogen for IIb-IIIa, on the development of isometric tension and ultrastructure of platelet-fibrin clots. Monoclonal antibodies A2A6 and 7E3 decreased the maximal tension, as well as the rate of tension development. Platelets and fibrin were oriented longitudinally in antibody treated clots, but the concentrations of fibrin and platelet aggregates determined by morphometry were significantly reduced. T10 and 10E5 increased tension, while AP2 and PAC1 had no substantial effect. Increasing concentrations of RGDS from 62.5 pM to 500 pM resulted in greater maximal tension and rate of tension development, reaching a five-fold increase when 500 pM RGDS was used. RGDS did not affect the Mg-stimulated platelet actomyosin ATPase activity. Morphometry revealed increased concentrations of oriented fibrin and platelet aggregates in RGDS-treated clots. Results of this study confirm the different topography of the epitopes on the Ilb-IIIa complex and provide evidence for different receptor sites for fibrinogen and fibrin on the IIb-IIIa complex

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D’Souza, S. E., M. H. Ginaberg, S. Lam, and E. A. Plow. "ACTIVATION DEPENDENT ALTERATIONS IN THE CHEMICAL CROSSLINKING OF ARGINYL-GLYCYL-ASPARTIC ACID (RGD) PEPTIDES WITH PLATELET GLYCOPROTEIN (GP) GPIIb-IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643699.

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The platelet adhesive proteins, fibrinogen, fibronectin and von WillebrandFactor, contain RGD amino acid sequences; RGD-containing peptides inhibit the binding of these adhesive proteins to platelets; and a membrane receptor for these adhesive proteins binds to Arg-Gly-Asp and contains GPIIb-IIIa. The present study was undertaken to characterize the interaction of RGDpeptides with GPIIb-IIIa using a chemical crosslinking approach. A radioiodinated RGD-containing heptapeptide was bound to washed human platelets under conditions at which ≥ 85% of theinteraction was inhibited by excess nonlabeled peptide. After binding of the peptide to platelets for 45 min at22°, a homobifunctional crosslinking reagent was added, and the platelets were extracted and analyzed on polyacrylamide gels. With resting platelets,autoradiography of the gels revealedthat the peptide crosslinked tobothGPIIb and GPIIIa. This interaction wasinhibited by excess nonlabeled peptide but not by certain conservatively substituted RGD peptides. Stimulation of the platelets caused a dramatic increase in crosslinking of the peptide to only one of the two subunitsof GPIIb-IIIa. The stimulus dependentincrease in the crosslinking reactionwas specific and saturable as it was inhibited by RGD peptides in a dose dependent manner. In addition, peptides corresponding in structure to the carboxy terminus of the γ chain of fibrinogen also produced concentration dependent inhibition of the interaction. The increase in crosslinking induced by platelet stimulation was divalent ion dependent. Similar results werealso obtained with a second, larger RGD-containing peptide and with asecond chemical crosslinking reagent.Theseresults indicate that platelet stimulation in the presence of divalent ions causes a change which permitsmoreefficient crosslinking of RGD-containing peptides to only one of the two subunits of GPIIb-IIIa. The results are also compatible with a proximalrelationship of both subunits tothe RGD binding sites on the plateletmembrane.

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Gustafson, E. J., H. Lukasiewicz, A. H. Schmaier, S. Niewiarowski, and R. W. Colman. "FIBRINOGEN BINDS TO HUMAN NEUTROPHILS AT A SITE DISTINCT FROM GPIIb/IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643850.

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Many observations suggest a potential role for neutrophils in the modulation of hemostasis and thrombosis. Arterial thrombi are characterized by the presence of large numbers of neutrophils lining the perimeter of platelet aggregates. While investigating binding of high molecular weight kininogen (HMWK) to neutrophils, we found that fibrinogen (Fb) could inhibit binding of 125I-HMWK as well as displace HMWK already bound to neutrophils. We therefore initiated studies to determine whether Fb could bind to human neutrophils. Both Zn++ and Ca++ were required for maximal binding of 125I-Fb to neutrophils. Binding did not occur with Ca++ (ZmM) alone and was only 1/3 the maximal amount with Zn++ (50 μM) alone. At 4° the amount of 125I-Fb bound to neutrophils reached a plateau by 15 minutes and remained at this level over the next 30 minutes. At 23° and 37° the amount of 125I-Fb bound peaked by 4 minutes and then decreased over the next 30 minutes indicating receptor-mediated internalization. Excess Fb inhibited binding of 125I-Fb to neutrophils while prekal1ikrein, factor XII, and fibronectin did not. Binding of 125I-Fb was 99% reversible at 4° within 10 minutes with a 50-fold molar excess of Fb and 90% displaceable by excess HMWK. The apparent Kd was approximately 0.45 μM. Arg-Gly-Asp-Ser (RGDS) is a tetrapeptide common to Fb, fibronectin, vitronectin and other cel 1-attachment proteins. Fb has been demonstrated to bind to the glycoprotein IIb/111 a (GPIIb/IIIa) complex which is the platelet membrane receptor for RGDS. Although this RGDS-GPIIb/IIIa interaction occurs with Fb binding to platelets, it is apparently not involved with Fb binding to monocytes. To investigate if Fb binding to neutrophils involved this interaction of GPIIb/II la -RGDS we performed further studies. Binding of 125I-Fb to neutrophils was not inhibited by RGDS nor was it inhibited by a monoclonal antibody (10E5) to the platelet GPI I b/IIIa complex. In addition, the amount of 125I-Fb that hound to neutrophils from a patient with Glanzman's thrombosthenia was the same as that bound to normal neutrophils. These studies indicate that human neutrophils specifically bind Fb at a site similar to HMWK and distinct from GPIIb/IIIa.

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Fresslnaud, E., J. E. Sadler, J. P. Girma, H. R. Baumgartner, and D. Meyer. "SYNTHETIC RGD-CONTAINING PEPTIDES OF VON WILLEBRAND FACTOR INHIBIT PLATELET ADHESION TO COLLAGEN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643591.

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The Arg-Gly-Asp (RGD) sequence is common to fibrinogen (Fg), fibronectin (Fn) and von Willebrand Factor (vWF). RGD-containing peptides compete for binding of these adhesive proteins to platelet membrane GPIIb/IIIa and inhibit thrombin-induced platelet aggregation as does an unrelated dodecapeptide from the γ Fg COOH terminus (γFg 400-411). We compared in flowing blood the effect of γ Fg 400-411 and of 3 synthetic peptides derived from the sequence of human vWF upon platelet adhesion to collagen. The 3 vWF peptides (13 or 18 aminoacids) contained an RGD sequence in the NH2 (peptide 03), central (peptide 07) or COOH (peptide 02) portions. Collagen was coated onto plastic coverslips and exposed in a parallel-plate perfusion chamber to reconstituted human blood at a shear rate of 2,600 s™1 for 3 min at 37°C. Perfusates contained physiological concentrations of 51 Cr-platelets and red cells in either citrated autologous plasma or modified Tyrode buffer containing 4% human albumin ; in the latter case, the collagen-coated coverslips were preincubated with normal plasma or purified human vWF prior to perfusion. Platelet-collagen interactions were estimated by radioactivity counting and quantitative morphometry. RGD peptides 02, 03 and 07 inhibited platelet-collagen interactions in a dose-dependent manner. With peptide 07, deposition of 51 Cr-platelets decreased from 283.8 ± 32.5 × 105/cm2 (mean ± SEM, n = 3) with buffer to 169.6 ± 33.0 in the presence of 50 μM peptide (p < 0.05), 133.7 ± 26.4 with 150 uM (p <0.012) and 101.8 ± 27.1 with 300 uM (p <0.005). The inhibitory effect of γ Fg 400-411 upon platelet deposition was less significant than that of the RGD peptides at 50 and 150 uM concentrations (224.4 ± 39.8, N.S. and 139.5 ± 55.3, p < 0.05, respectively). RGD peptide 07 also inhibited in a dose-dependent way both platelet adhesion to collagen and thrombus growth. Similar results were observed with peptides 02 and 03, indicating that the position of the RGD sequence is not critical. No synergetic effect between RGD and γFg 400-411 peptides was observed. These results with vWF peptides confirm that GPIIb/IIIa is involved not only In platelet aggregation (thrombus growth) but also in vWF-mediated platelet adhesion to collagen.

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Reports on the topic "RGDP"

1

Casal, Ignacio. Metástasis, integrinas y cadherinas RGD. Sociedad Española de Bioquímica y Biología Molecular (SEBBM), November 2016. http://dx.doi.org/10.18567/sebbmdiv_anc.2016.11.1.

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2

Hint, C. RGD # A-9 Complete Characterization. Office of Scientific and Technical Information (OSTI), September 2020. http://dx.doi.org/10.2172/1682520.

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3

Wu, I., and T. Eckert. Cisco Systems Router-port Group Management Protocol (RGMP). RFC Editor, February 2003. http://dx.doi.org/10.17487/rfc3488.

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4

Liu, Shuang. Novel Approach to Prepare {sup 99m}Tc-Based Multivalent RGD Peptides. Office of Scientific and Technical Information (OSTI), October 2012. http://dx.doi.org/10.2172/1053772.

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