Journal articles: 'Rh negativo'

1

Bortolotto, Adriana Najai, Márcia Maria Mikalauscas, Anelise Levay Murari, Samara Rubin, and José Edson Paz da Silva. " ESTUDO E PREVALÊNCIA DO SISTEMA RH E KELL NOS DOADORES DO HEMOCENTRO DE SANTA MARIA-RS ." Saúde (Santa Maria) 37, no. 2 (April 26, 2012): 49. http://dx.doi.org/10.5902/223658343025.

Full text

Abstract:

O trabalho teve como objetivo avaliar a prevalência dos fenótipos do sistema Rh e Kell em doadores do Hemocentro de Santa Maria. A frequência destes sistemas depende da etnia de cada região. Os principais antígenos do sistema Rh incluem: D, C/c/Cw, E/e. O sistema Kell foi descoberto em 1946, depois da introdução do teste de antiglobulina. Os anticorpos podem causar Doença Hemolítica do Recém-Nascido. Das 1832 amostras fenotipadas , quanto ao sistema Rh, 870 amostras (48%) foram fenotipadas como Rh positivos e 962 amostras (52,0%) fenotipadas como Rh negativo. Das fenotipadas como Rh negativas, 78 amostras (8,1%) foram positivas para o antígeno “C” e/ou “E”. Relacionando o percentual do sistema Kell positivo e Rh negativos foi de 8,2%. O doador Rh negativo deve ser analisado, para os demais antígenos deste sistemas e para o antígeno Kell , mesmo sendo menos imunogênicos, são capazes de causar Doença Hemolítica Grave e Aloimunizações. Descritores: Fenotipagem, Freqüência, Doadores, Sistema Kell, Sistema Rh.

APA, Harvard, Vancouver, ISO, and other styles

2

Benavides Lozada, Benjamin, and Jorge Berrocal. "INCOMPATIBILIDAD SANGUÍNEA ABO Y RH NEGATIVO EN GESTANTES ATENDIDAS EN EL HOSPITAL MILITAR CENTRAL DURANTE UN DECENIO." Revista Peruana de Ginecología y Obstetricia 22, no. 3 (May 30, 2015): 127–35. http://dx.doi.org/10.31403/rpgo.v22i704.

Full text

Abstract:

Durante el período del presente trabajo, en el HMC se atendieron 77 g estantes en 118 gestaciones, con incompatibilidad sanguínea de grupo y/o factor Rh materno-fetal. Se encontraron tres tipos de incompatibilidad: Factor Rh, ABO y Mixta (ABO-RH(-) simultánea), resultando más frecuente el primer tipo.

APA, Harvard, Vancouver, ISO, and other styles

3

Brítez, Myrian Beatriz, Alcides Alejandro Ortiz, and Gloria Martínez. "Protocolo de informe para pacientes con presunción de presencia de variante factor Rh d / du positivo." Revista Científica Estudios e Investigaciones 9 (May 3, 2021): 153–54. http://dx.doi.org/10.26885/rcei.foro.2020.153.

Full text

Abstract:

Para realizar pruebas serológicas de grupo sanguíneo son necesarios buenos conocimientos, tanto teórico como práctico. En la formación del personal debe hacerse también hincapié en las precauciones necesarias para reducir el riesgo de errores de manipulación, así como en las técnicas a ser aplicada. Un error de manipulación en la transfusión de sangre puede ser una importante causa de daños o incluso defunción. El antígeno Du es una variante débil del antígeno D que tiene interés desde el punto de vista transfusional. Este antígeno aglutina débilmente o no aglutina con los anticuerpos Anti-D habituales y, por tanto, es posible clasificar erróneamente al portador de este antígeno como Rh negativo. La transfusión de esta sangre a una persona Rh negativo produciría sensibilización Anti-D. Por este motivo, siempre ante un Rh negativo se investiga la presencia del antígeno Du mediante el Test de Coombs. El antígeno D incluye los tipos de categoría D, D parcial y D débil, que son importantes porque la a la inmunización anti-D puede ocurrir en algunas personas, pero no en todas, que expresan un alelo Rh D variante. En la actualidad, existe poca información prospectiva sobre la prevalencia de variantes D entre pacientes obstétricas y potenciales receptores de transfusiones.

APA, Harvard, Vancouver, ISO, and other styles

4

Bezerra, Ana Katarina Moraes Monteiro, and Fabrício Andrade Martins Esteves. "Influência da classificação sanguínea realizada em lâmina sobre a administração profilática de imunoglobulina anti-D em pacientes obstétricas." Revista de Ciências Médicas e Biológicas 9, no. 3 (January 1, 2010): 189. http://dx.doi.org/10.9771/cmbio.v9i3.5158.

Full text

Abstract:

Cerca de 15% das mulheres são Rh negativas e podem ser sensibilizadas por seus filhos Rh positivos. A isoimunização RhD pode ser prevenida pela administração de imunoglobulina anti-D. Por esse motivo, é especialmente na população obstétrica que a classificação Rh não pode deixar dúvidas. Em 0.7% dessas classificações são encontradas hemácias D fraco, que têm uma redução no número de moléculas D na sua superfície. Dessa forma, visamos a determinar a possível presença do antígeno D fraco em gestantes, puérperas e neonatos que foram atendidos por uma maternidade no município de Caruaru (PE) e classificados como RhD negativos. Testes de Coombs indireto e direto também foram realizados para avaliar a presença de anticorpos irregulares nas gestantes e a sensibilização eritrocitária nos neonatos. Este estudo foi do tipo descritivo no qual avaliamos 29 gestantes puérperas e 14 neonatos, que foram classificadas para os sistemas ABO e RhD por meio de teste de aglutinação em lâmina. As amostras foram enviadas ao laboratório da ASCES, onde foi realizada a classificação sanguínea por meio de teste de aglutinação em tubo e pesquisa do antígeno D fraco naqueles casos que foram Rh negativos confirmados. Foram encontradas duas mães que eram D fraco, uma mãe com teste de Coombs indireto positivo e um neonato que é Rh positivo, mas tinha sido classificado como Rh negativo. Para que se possa prevenir a isoimunização RhD, é necessária a realização da classificação sanguínea e pesquisa do antígeno D fraco em gestantes, puérperas e neonatos, de modo que seu resultado seja confiável.

APA, Harvard, Vancouver, ISO, and other styles

5

Medeiros, Raquel Trovão de, Renato Galvão Bezerra, Rejane Maria Paiva de Menezes, Rejane Marie Barbosa Davim, and Camila Fernandes da Silva Carvalho. "The use of the Rogan vaccine during the prenatal in Rh-negative women: knowledge of health professionals." Revista de Enfermagem UFPE on line 5, no. 5 (June 26, 2011): 1193. http://dx.doi.org/10.5205/reuol.1302-9310-2-le.0505201115.

Full text

Abstract:

ABSTRACTObjective: to identify the nurse and physician knowledge on the importance of using anti-Rh vaccine during the prenatal care of pregnant women with Rh-negative blood types. Method: this is a descriptive and exploratory study, with a quantitative approach, developed along with physicians and nurses who work in prenatal care services in hospitals and in the Family Health Program (FHP) in the towns of Bento Fernandes and Santo Antonio, in the agreste region of the State of Rio Grande do Norte, Brazil. The study was approved by the Committee of Ethics in Research of Universidade Federal do Rio Grande do Norte with a Favorable Recommendation under the Protocol 101/06 CEP-UFRN. Results: there is a need of efforts from all healthcare professionals in order to place a greater emphasis on isoimmunization and potential perinatal hemolytic disease in the prenatal care services, aiming to avoid future complications for the mother and child. Conclusion: one can realize there is not a more effective action provided by the professionals; this is not due to the lack of information on the disease and/or vaccine, but it occurs, especially, because they do not know how to use the vaccine when pregnant women with Rh-negative blood are identified during the prenatal care. Descriptors: nursing; prenatal; pregnancy; Rh isoimmunization; prevention; women’s health.RESUMOObjetivo: identificar o conhecimento do enfermeiro e médico acerca da importância da utilização da vacina anti-Rh durante o pré-natal em gestantes portadoras de Rh negativo. Método: trata-se de um estudo do tipo exploratório descritivo, com abordagem quantitativa, desenvolvido com médicos e enfermeiros que trabalham em serviços de pré-natal em instituições hospitalares e no Programa Saúde da Família (PSF) nos municípios de Bento Fernandes e Santo Antônio, localizados na região agreste do Estado do Rio Grande do Norte. O estudo foi aprovado pelo Comitê de Ética em Pesquisa da Universidade Federal do Rio Grande do Norte com Parecer Favorável e Protocolo n. 101/06 CEP-UFRN. Resultados: é necessário que haja esforços de todos os profissionais da saúde no sentido de dar maior ênfase à isoimunização e possível doença hemolítica perinatal nos serviços de assistência pré-natal, com a finalidade de evitar futuras complicações para mãe e bebê. Conclusão: percebe-se que não há ação mais efetiva por parte dos profissionais, não em função da falta de informações sobre a doença e/ou vacina, mas, principalmente, porque não é do conhecimento a utilização da vacina quando identificam gestantes Rh negativo durante o pré-natal. Descritores: enfermagem; pré-natal; gestação; isoimunização Rh; prevenção; saúde da mulher.RESUMENObjetivo: identificar el conocimiento del enfermero y del médico acerca de la importancia de la utilización de la vacuna anti-Rh durante el prenatal en gestantes portadoras de Rh negativo. Método: se trata de un estudio del tipo exploratorio-descriptivo, con abordaje cuantitativa, desarrollado con médicos y enfermeros que trabajan en el prenatal en institutos hospitalares y en el Programa Salud de la Familia (PSF) en los municipios de Bento Fernandes y Santo Antônio, localizados en la región agreste del Estado do Rio Grande do Norte, Brasil. El estudio fue aprobado por el Comité de Ética en Investigación de la Universidade Federal do Rio Grande do Norte con Parecer Favorable y Protocolo 101/06 CEP-UFRN. Resultados: es necessario que haya esfuerzo de todos los profesionales de la salud para dar más énfasis a la isoimunización y possible enfermedad hemolítica perinatal en los servicios de asistencia prenatal, con la finalidad de evitar futuras complicaciones para la madre y su hijo. Conclusión: se percebe que no hay acción más efectiva por parte de los profesionales, esto no se da por falta de informaciones relativas a la enfermedad y/o vacuna, pero, principalmente, porque ellos no conocen la utilización de la vacuna cuando identifican gestantes Rh negativo durante el prenatal. Descriptores: enfermería; prenatal; gestación; isoimunización Rh; prevención; salud de la mujer.

APA, Harvard, Vancouver, ISO, and other styles

6

Cruz, Harold Fabián, Jorge Enrique Moreno Collazo, and Sandra Erika Forero. "Caracterización de donantes voluntarios de sangre por grupo sanguíneo A B O y Rh que asistieron a un banco de sangre de la ciudad de Tunja- Colombia./Characterization of voluntary blood donors for blood group ABO and Rh attending a blood bank in the city." Archivos de Medicina (Manizales) 12, no. 2 (December 15, 2012): 185–89. http://dx.doi.org/10.30554/archmed.12.2.7.2012.

Full text

Abstract:

Objetivo: La frecuencia de grupo sanguíneo ABO y factor Rh es un factor importante en relación a las necesidades de los componentes sanguíneos en la población, la identificación un procedimiento de rutina en los bancos de sangre.Materiales y métodos: Se realizó un estudio retrospectivo de corte trasversal descriptivo, periodo entre enero a marzo de 2012 con datos proporcionados por la Fundación Hematológica Colombia provenientes de donantes voluntarios de sangre que asistieron a un punto fijo de recolección de sangre de la ciudad de Tunja – Colombia. Las variables analizadas fueron: edad, género, factor Rh y grupo sanguíneo ABO.Resultados: La población de estudio estuvo conformada por 1678 donantes voluntarios la edad promedio de 28,29 años, el 53,3 % (n=894), 62,9% de la población pertenece al grupo O, se encontró mayor proporción de Rh positivo frente al negativo (94,9 vs 5,1), el tipo de sangre O positivo es el 32% (n=532) del total de la población.Conclusiones: El grupo O y A son los que predominan en la población de estudio, comportamiento similar a loa reportado en la literatura, de igual manera el Rh negativo.Background: The frequency of ABO blood group and Rh factor is an important factorin relation to the needs of blood components in the population, identifying a routine inblood banks.Materials and methods: A retrospective, cross sectional, descriptive study was performed,period from January to March 2012 with data provided by the Foundation fromColombia Hematological volunteer blood donors who attended a fixed point of bloodcollection in the city of Tunja - Colombia. The variables analyzed were age, gender,and Rh blood group ABO.Results: The study population consisted of volunteer donors 1678 the average ageof 28,29 years, 53,3% (n = 894), 62,9% of the population belongs to the group O,there was higher proportion of positive versus negative Rh (94,9 vs 5,1 ), blood typeO positive, 32% (n = 532) of the total population.Discussion: The group O and A are predominant in the study population, similar tobehavior reported in the literature loa, just as the Rh negative.

APA, Harvard, Vancouver, ISO, and other styles

7

Quispe A., Peter, Enrique León M., and Juan M. Parreño T. "Frecuencia de los sistemas ABO y RH en personas que acudieron al servicio académico asistencial de análisis clínicos." Ciencia e Investigación 11, no. 1 (June 16, 2008): 42–49. http://dx.doi.org/10.15381/ci.v11i1.4920.

Full text

Abstract:

Se determinaron los grupos sanguíneos de los sistemas ABO y factor Rh en 3655 personas que acudieron al Servicio Académico Asistencial de Análisis Clínicos de la Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, entre los años 2003-2005, con la intención de saber la frecuencia de los mismos, y conocer nuestras peculiaridades de tipos sanguíneos, además de comparar nuestras frecuencias con otras investigaciones. Se utilizó reactivos antisueros estandarizados siguiendo las técnicas convencionales. El análisis estadístico de los resultados en los grupos sanguíneos del sistema ABO y Factor Rh nos dio: "O" Positivo 73.36%, "O" Negativo 0.38%, "A". Positivo 18.85%, "A" negativo 0.27%, "B" Positivo 5.85% y" AB'' positivo 1.45%, estas frecuencias variaron según la región de procedencia de las personas involucradas (Costa, Sierra y Selva), así mismo se encontró ligeras variaciones al comparar con otros estudios que se realizaron en el transcurso de anteriores años.

APA, Harvard, Vancouver, ISO, and other styles

8

Nardozza, Luciano Marcondes Machado, Luiz Camano, Antonio Fernandes Moron, David Baptista da Silva Pares, Paulo Alexandre Chinen, and Guilherme Antonio Rago Lobo. "Alterações ultra-sonográficas na gravidez Rh negativo sensibilizada avaliada pela espectrofotometria do líquido amniótico e pela dopplervelocimetria da artéria cerebral média." Radiologia Brasileira 39, no. 1 (February 2006): 11–13. http://dx.doi.org/10.1590/s0100-39842006000100004.

Full text

Abstract:

OBJETIVO: Avaliar e confrontar a presença de alterações ultra-sonográficas nas gestações Rh negativo sensibilizadas, quando a anemia fetal foi determinada ou pela espectrofotometria do líquido amniótico, ou pela dopplervelocimetria da artéria cerebral média. MATERIAIS E MÉTODOS: Observacional descritivo com grupo de comparação. Nosso grupo de estudo foi constituído por 99 pacientes, avaliadas no período de janeiro de 1995 a janeiro de 2004. Foram analisados e comparados dois grupos: 74 gestantes sensibilizadas pelo fator Rh cuja anemia fetal foi acompanhada pela espectrofotometria (grupo SE) e 25 gestantes sensibilizadas pelo fator Rh cuja anemia fetal foi acompanhada pela dopplervelocimetria (grupo SD). Avaliamos a presença ou não de alterações ultra-sonográficas no acompanhamento pré-natal e confrontamos os dois grupos de estudo. RESULTADOS: No grupo cuja anemia fetal foi acompanhada através da espectrofotometria (grupo SE), apuramos modificações placentárias, principalmente o aumento da espessura e sua alteração textural, mais assiduamente que as encontradiças no grupo de gestantes sensibilizadas, em que a anemia foi determinada através da dopplervelocimetria (grupo SD) (64% X 32%, p = 6,294). CONCLUSÃO: As alterações ultra-sonográficas foram detectadas em dobro quando a anemia foi avaliada pela espectrofotometria em comparação com o grupo seguido pela dopplervelocimetria.

APA, Harvard, Vancouver, ISO, and other styles

9

Mota, Lennara Pereira, Simone Barbosa Pereira, Geórgia Maria Vaz Feitosa do Vale, Rosa Adélia Machado de Carvalho, Lillian Monizy Mesquita Carlos, Daiane Borges Souza, Isadora Lima de Souza, et al. "Sistema Rh e associação com a doença hemolítica do recém-nascido." Research, Society and Development 9, no. 9 (August 20, 2020): e332996950. http://dx.doi.org/10.33448/rsd-v9i9.6850.

Full text

Abstract:

Os antígenos D, C, c, E, e, que estão localizados em duas proteínas expressas na membrana das hemácias: RhD (CD240D) e RhCE (CD240CE). A primeira proteína está relacionada ao antígeno D (Rh1) e suas variantes, a segunda aos antígenos C, E, c, e (Rh2 a Rh5) em diversas combinações (CE, cE, Ce e ce) e variantes. O sistema Rh é formado por mais de 56 antígenos capazes de produzir a doença hemolítica. Os antígenos D, C, c, E, são os principais e mais importantes antígenos e, portanto, chamados de sistema, com base na nomenclatura descrita por Rosenfield. O trabalho tem por objetivo analisar através de publicações cientificas a associação do sistema Rh com a doença hemolítica do recém-nascido. Trata-se de uma revisão bibliográfica de caráter qualitativo que se baseia na produção científica a partir de estudos já publicados. A DHRN caracteriza-se pela destruição dos eritrócitos fetais por anticorpos de classe IgG presentes na circulação materna. Esses anticorpos específicos contra antígenos presentes nas hemácias do feto penetram a barreira placentária e promovem o processo hemolítico prematuro dos eritrócitos, causando à anemia fetal. Essa anemia dispõe de graus que variam de acordo com a intensidade da destruição das hemácias. A DHRN pode apresentar casos graves levando a anemia, aumento da bilirrubina e comprometimento de órgãos e por isso é necessário que mães Rh negativo utilizem medidas de imunoprevenção com o uso de imunoglobulina anti-D policlonal.

APA, Harvard, Vancouver, ISO, and other styles

10

Fernandes, Aida Pinto, Claudia Marques de Oliveira Soeiro, Fernanda Araújo Ribeiro, Kassya da Silva Rebelo, and Glauber Palma de Oliveira. "Prevalência de isoimunização Rh materna em maternidade pública do Amazonas entre 2018 e 2020." Revista Eletrônica Acervo Saúde 13, no. 9 (September 17, 2021): e8802. http://dx.doi.org/10.25248/reas.e8802.2021.

Full text

Abstract:

Objetivo: Analisar a prevalência de isoimunização Rh materna no período de 2018 a 2020 em maternidade do Amazonas. Métodos: Foi realizado um estudo observacional e descritivo retrospectivo de casos de sensibilização materna a Rh(D) atendidos em uma maternidade pública na cidade de Manaus, Amazonas. Foram utilizados dados secundários a partir do prontuário para análise de variáveis como tipagem sanguínea e fator RhD, idade, raça, idade gestacional, paridade, testes de controle Coombs. Esses prontuários estavam catalogados no Serviço de Atendimento Médico e de Emergência (SAME) no período de estudo. Resultados: A prevalência foi de 1,49% dos casos notificados. A média de idade da gestante foi de 25,17 anos, sendo 63% das pacientes de cor parda. A tipagem sanguínea, foi de 61,2% das mulheres do tipo O negativo e os recém-nascidos, 41,5% O positivo. A média de consultas de pré-natal foi de apenas 5,3 consultas. Conclusão: A taxa de prevalência encontrada, apesar de baixa, poderia ser ainda menor, uma vez que na maioria das mulheres da Região Norte outros tipos sanguíneos são mais prevalentes. O pré-natal com número reduzido de consultas pode ter sido fator responsável pela falha da prevenção da Doença Hemolítica perinatal no Estado.

APA, Harvard, Vancouver, ISO, and other styles

11

Ponce de León Bardales, Oscar. "EMBARAZO DE ALTO RIESGO." Revista Peruana de Ginecología y Obstetricia 30, no. 2 (May 17, 2015): 37–42. http://dx.doi.org/10.31403/rpgo.v30i624.

Full text

Abstract:

De 1632 gestantes atendidas por el autor, 437 (26.7%) eran portadores de riesgo obstétrico y 186 (11 .4%) de riesgo intraparto. Aquéllas con riesgo obstétrico en el pre natal, el 32% presentaban complicaciones médicas (várices, tuberculosis pulmonar, infecciones urinarias, factor Rh negativo, endocrinopatías) y el 40% antecedentes obstétricos de importancia (cesárea, multiparidad. Los factores de riesgo intraparto más importantes constituyeron la ruptura prematura de membranas, el sufrimiento fetal agudo y el trabajo de parto disfuncional. Mientras que sólo el 13.7% de toda la población estudiada tuvo su bebé por cesárea, la incidencia de cesáreas en todas las gestantes de alto riesgo fue 35 .6%. Hubo un 52 % de infecciones puerperales en el segundo grupo. La mortalidad perinatal fue 27% N .V. en la población general y 64% N .V. en la de alto riesgo.

APA, Harvard, Vancouver, ISO, and other styles

12

Quintero-Ortíz, María Andrea, Ximena Briceño-Morales, Oswaldo Sanchez-Castillo, Juan Carlos Velasquez, Carlos Bonilla Gonzalez, Luis Guzman-Abisaab, Sergio Cervera-Bonilla, et al. "Hormonoterapia en cáncer de mama metastásico. Revisión de la Evidencia y Abordaje Terapéutico en el Instituto Nacional de Cancerología, Bogotá - Colombia." Revista Colombiana de Cancerología 25 (August 5, 2021): 142–51. http://dx.doi.org/10.35509/01239015.747.

Full text

Abstract:

El 16.9% de las pacientes con cáncer de mama, que ingresan a la Unidad Funcional de Mama y Tejidos Blandos del Instituto Nacional de Cancerología (INC), se presentan con enfermedad metastásica. El desarrollo de las terapias blanco ha modificado radicalmente el enfoque terapéutico en este grupo de pacientes. Se realizó una búsqueda de la literatura seleccionando los ensayos clínicos controlados y aleatorizados fase 3, las revisiones sistemáticas y los metaanálisis, sobre el tratamiento sistémico para el cáncer de mama metastásico con receptores hormonales positivos (RH+) y receptor HER2 negativo (HER2).Se pusieron filtros a la búsqueda para identificar únicamente los artículos publicados a noviembre de 2020 y en idioma inglés. Posteriormente, se socializó la revisión de la evidencia al interior de las Unidades Funcionales (UF) de Mama y Tejidos Blandos y Oncología clínica. Finalmente, se realizó una discusión académica en la cual se establecieron los cambios en el abordaje terapéutico de las pacientes con cáncer de mama luminal, HER2 negativo, metastásico. En los últimos años, el conocimiento de la biología molecular del cáncer de mama ha permitido el desarrollo de múltiples terapias blanco (iCDK4/6, iPI3KCA, inhibidores del mTOR), que combinadas con la terapia hormonal, mejoran los desenlaces oncológicos de las pacientes con enfermedad metastásica (supervivencia libre de progresión y supervivencia global).

APA, Harvard, Vancouver, ISO, and other styles

13

Santos, Rodrigo Amado dos, Ruan Santana Montenegro de Almeida, and Luiz Felipe Miranda. "A sustentabilidade e a hotelaria carioca: Critérios para um desenvolvimento integrado e participativo." Turismo - Visão e Ação 23, no. 1 (February 25, 2021): 191–215. http://dx.doi.org/10.14210/rtva.v23n1.p191-215.

Full text

Abstract:

Os debates sobre gestões sustentáveis tornam-se notórios à contemporaneidade. Essa perspectiva é justificada graças aos gestores necessitarem lidar: com um público-alvo mais consciente dos limites a serem impostos ao uso dos recursos naturais/culturais; e com o impacto negativo de suas operacionalizações sobre o meio ambiente e a sociedade. Essa premissa é ainda mais imperativa em atividades cujo poder de transformação socioambiental, cultural e econômico é cada vez mais latente, como é o caso da hotelaria. Desse modo, objetivou-se apresentar procedimentos, técnicas e/ou condutas necessários para a execução de 39 critérios para a promoção de uma hotelaria sustentável. Assim, um estudo qualitativo foi elaborado, embasado nas métricas de multicaso e entrevistas em profundidade. Participaram destas entrevistas três gestores hoteleiros – 02 gerentes gerais e 01 supervisora de RH – e um representante do Ministério do Turismo, coordenador do Grupo Turismo Sustentável – ISO. A partir de suas experiências, foram estruturadas descrições que auxiliarão a operacionalização de cada critério, de forma a garantir uma gestão hoteleira holística, integrada e participativa.

APA, Harvard, Vancouver, ISO, and other styles

14

CONTRERAS, MARCELA, and ROBIN C. KNIGHT. "The Rh-negative donor." Clinical & Laboratory Haematology 11, no. 4 (December 1989): 317–22. http://dx.doi.org/10.1111/j.1365-2257.1989.tb00229.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Dean, Mark. "Wanted: Rh D negative donors." Australian Prescriber 23, no. 2 (April 1, 2000): 36–38. http://dx.doi.org/10.18773/austprescr.2000.037.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Namdhari, Balkrishna H., Tapasya V. Bharati, and Ashish P. Shinde. "Wastage of rarest blood and blood components can be stopped: 9 years retrospective and cross-sectional study at tertiary hospital." International Journal of Research in Medical Sciences 6, no. 11 (October 25, 2018): 3678. http://dx.doi.org/10.18203/2320-6012.ijrms20184429.

Full text

Abstract:

Background: Inventory of blood/blood components suffer due to outdating of Rh Negative units. Rationale of this article is strategy of transfusion of O Rh Negative red cells to all. A Rh Negative red cells can be given to A Rh Positive, AB Rh Positive. B Rh Negative red cells can be given to B Rh Positive, AB Rh Positive. AB Rh Negative red cells to AB Rh Positive recipients. AB Rh Negative FFP/PRP can be given to all. Objective was to avoid out dating of Rh Negatives by studying the percentage of outdated Rh Negative units amongst the all outdated.Methods: This was 9 years observational, retrospective, cross sectional and descriptive study conducted at tertiary care hospital. Outdated units of Rh Negative blood and components were analysed from the year wise discard registers of blood bank. Percentage of Rh Negative units within all outdated units were calculated.Results: 198 Rh Negative units of whole blood and blood components within all outdated units was 29.11%. Out of 198 the 20 Rh Negative blood components were discarded.Conclusions: Adopt type and screen protocol to prevent outdating. Avoid to bleed the rare blood groups. Audit by hospital transfusion committee and implementation of MSBOS. Track O Rh Negative red cells transfusion to Rh Positive as quality indicator. Track/review transfusion of O Rh negative red cells to Non O Rh negative recipients. Track AB Rh Negative FFP/PRP transfusions to all.

APA, Harvard, Vancouver, ISO, and other styles

17

Kobayashi, Kiyoko, Shinji Matsumoto, Mizuki Arai, Yasuka Watanabe, Mai Maeda, Takashi Tanazawa, Sachiko Fukunaga, Mitsue Hirayama, and Kenji Ikebuchi. "ANTI-D ANTIBODY DETECTION AFTER TRANSFUSION OF RhD-NEGATIVE RBCs IN AN RhD-NEGATIVE RECIPIENT." Japanese Journal of Transfusion and Cell Therapy 61, no. 3 (2015): 416–21. http://dx.doi.org/10.3925/jjtc.61.416.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Cionca, Octavia, Mihaela Zahner, Z. Hadnagy, O. Bonţe, and A. Murariu. "Rh incompatibility and the pregnancy outcome of Rh negative women." Obstetrica şi Ginecologia 2, no. 67 (2019): 66. http://dx.doi.org/10.26416/obsgin.67.2.2019.2419.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Wah, Saw Thu, Saung Nay Chi, Kyi Kyi Kyaing, Aye Aye Khin, and Thida Aung. "Serological Detection of Rh-Del Phenotype among Rh-Negative Blood Donors at National Blood Center, Yangon, Myanmar." Advances in Hematology 2020 (February 18, 2020): 1–5. http://dx.doi.org/10.1155/2020/3482124.

Full text

Abstract:

Background. Red cell Rhesus (Rh) antigen expression is influenced by the genetic polymorphism of RHD and RHCE genes and reveals serologically different reactions of RhD variants such as partial D, weak D, and Rh-Del. Serologically, Rh-Del type can only be detected by an adsorption-elution technique, and it might be mistyped as Rh-negative. The prevalence of Rh-Del has not been reported yet in Myanmar. Method. A total of 222 Rh-negative blood donors in the National Blood Center were tested for weak D and Rh-Del by indirect antihuman globulin and adsorption-elution method, respectively. RhCE typing was performed among Rh-negative and Rh-Del. Results. Of them, 75.2% (167/222) were Rh-negative, 15.8% (35/222) were Rh-Del, and 9% (20/222) were weak D. Of 202 blood donors (167 true Rh-negative and 35 Rh-Del), all of the Rh-Del positives were C-antigen-positive with 94.3% Ccee phenotype (33/35) and 5.7% CCee (2/35). Most of the Rh-negative donors (80.2%) were ccee phenotype (134/167). Conclusion. About half of Rh-Del subjects were repeated donors, and attention was needed to avoid transfusion of truly Rh-negative patients to prevent alloimmunization. It is recommended to do Rh-Del typing of Rh-negative donors who are C-antigen-positive and consider moving them to the Rh-positive pool. Further study is needed to clarify the alloimmunization status for transfusion of Rh-Del blood to Rh-negative recipients. Molecular markers for RhD-negative and D variants should be established in the Myanmar population to improve selection of antisera for Rh typing and enhance safety of the transfusion services.

APA, Harvard, Vancouver, ISO, and other styles

20

Harrod, Kathryn Shisler, Lisa Hanson, Leona VandeVusse, and Patricia Heywood. "Rh Negative Status and Isoimmunization Update." Journal of Perinatal & Neonatal Nursing 17, no. 3 (July 2003): 166–80. http://dx.doi.org/10.1097/00005237-200307000-00002.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Hilden, Jörgen, and Folke Rønnike. "The Erythrocyte Sedimentation Rate in Rh-positive and Rh-Negative Donors." Scandinavian Journal of Haematology 12, no. 5 (April 24, 2009): 374–80. http://dx.doi.org/10.1111/j.1600-0609.1974.tb00224.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Tripathi, Rashmi, and Neelam Singh. "Maternal and perinatal outcome in Rh negative mothers." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 7, no. 8 (July 26, 2018): 3141. http://dx.doi.org/10.18203/2320-1770.ijrcog20183306.

Full text

Abstract:

Background: The objective of this study is to find out the incidence and foetomaternal outcome of Rh negative women during pregnancy.Methods: In the study group, the labor was monitored carefully and the mode of delivery and the outcome of labor was studied in detail. Baby was thoroughly examined for any obvious congenital anomaly, weight, sex and condition was also noted particularly for hydrops. If neonate was Rh positive, then the mother was given postpartum immunoprophylaxis within 24 hours of delivery. The new born were followed for 3 days and were watched for the development of Jaundice. Mothers were advised to attend postnatal clinic for check-up after 6 weeks of delivery.Results: Blood group distribution of newborn: 37 were Rh positive and 18 were Rh negative. Raised Rh antibody titre was not found in any of the 55 cases. Maximum cases 47 delivered at 38-40 weeks, 2 cases delivered after 40 weeks and 6 patients delivered between 30-38 weeks. Maximum cases 37 delivered normally, 12 required cesarean section and 2 had forceps delivery. The babies who developed NNHB were managed either by sunrays exposure only or by phototherapy. The babies who had anemia immediately after birth were carefully monitored and considered for exchange transfusion.Conclusions: Tremendous advances in the medical services and technology during the last few decades have revolutionized the treatment of Rh disease. Various studies have been conducted and several are going on the in this field to achieve zero incidence of this disease.

APA, Harvard, Vancouver, ISO, and other styles

23

Varvarigou-Frima, A., S. Mantagos, and NG Beratis. "Transformation of lymphocytes from immunized Rh(D)-negative subjects by Rh(D) isoantigen." Blood 72, no. 3 (September 1, 1988): 952–55. http://dx.doi.org/10.1182/blood.v72.3.952.952.

Full text

Abstract:

Abstract Since immune memory in Rh(D)-negative isoimmunized subjects remains through life, even in the absence of measurable anti-Rh(D), we investigated the transformation of lymphocytes from such donors by Rh(D) antigen. The time lapse from the last stimulus was up to 13 years. Mononuclear cells from immunized women were stimulated by Rh(D)- positive erythrocyte stroma. Maximum transformation was observed on the sixth day of culture, with a stroma protein concentration of 8 micrograms/mL of culture medium. The stimulation index (SI) in cells from 11 immunized women was 6.8 +/- 3.1 (mean +/- SD), with a range from 3.1 to 15.0. In five different sets of control cultures, the SI ranged from 0.9 +/- 0.2 to 1.3 +/- 0.4. There was no overlap between stimulated and control cultures. No anti-D could be demonstrated in the serum of four of the 11 immunized cases studied. Also, transformation was observed in mononuclear cells from Rh(D)-negative immunized women with Rh(D)-positive erythrocytes. The findings demonstrate that lymphocytes from isoimmunized Rh(D)-negative subjects maintain the immune memory and are transformed in vitro by the Rh(D) isoantigen.

APA, Harvard, Vancouver, ISO, and other styles

24

Varvarigou-Frima, A., S. Mantagos, and NG Beratis. "Transformation of lymphocytes from immunized Rh(D)-negative subjects by Rh(D) isoantigen." Blood 72, no. 3 (September 1, 1988): 952–55. http://dx.doi.org/10.1182/blood.v72.3.952.bloodjournal723952.

Full text

Abstract:

Since immune memory in Rh(D)-negative isoimmunized subjects remains through life, even in the absence of measurable anti-Rh(D), we investigated the transformation of lymphocytes from such donors by Rh(D) antigen. The time lapse from the last stimulus was up to 13 years. Mononuclear cells from immunized women were stimulated by Rh(D)- positive erythrocyte stroma. Maximum transformation was observed on the sixth day of culture, with a stroma protein concentration of 8 micrograms/mL of culture medium. The stimulation index (SI) in cells from 11 immunized women was 6.8 +/- 3.1 (mean +/- SD), with a range from 3.1 to 15.0. In five different sets of control cultures, the SI ranged from 0.9 +/- 0.2 to 1.3 +/- 0.4. There was no overlap between stimulated and control cultures. No anti-D could be demonstrated in the serum of four of the 11 immunized cases studied. Also, transformation was observed in mononuclear cells from Rh(D)-negative immunized women with Rh(D)-positive erythrocytes. The findings demonstrate that lymphocytes from isoimmunized Rh(D)-negative subjects maintain the immune memory and are transformed in vitro by the Rh(D) isoantigen.

APA, Harvard, Vancouver, ISO, and other styles

25

Khatun, Jamila, and Ruly Begum. "Effect of Rhesus Negative in Pregnancy." Medicine Today 30, no. 1 (February 1, 2018): 23–25. http://dx.doi.org/10.3329/medtoday.v30i1.35561.

Full text

Abstract:

Hemolytic disease of the newborn due to Rhisoimmunisation is a major cause to perinatal morbidity & mortality. Erythroblastosis fetalis is a disease of fetus and newborn due to incompatibility between fetal and maternal blood group. Diagnosis and therapy for Rh immunization improved considerably. Its prevention by immunoprophylaxis has been responsible for the reduction in the incidence of perinatal mobility. Still Rh immunization and erythroolastosis fetalis is responsible for many obstetric mishaps in our country. To see the pregnancy outcome of Rhesus negative women. This prospective study was carried out from October 2013 to March 2014 in Obstetrics & Gynecology department of Sylhet MAG Osmani Medical College & Hospital, Sylhet. 50 Rh-negative pregnancies were selected those who got admitted in department of Obstetrics & Gynecology, SOMCH. 30.62% of the fetuses had blood group B+ve, 24.40% 0+ve and 20.40% A+ve. Regarding the perinatal outcome 76% were healthy, 4% still birth, 4% neonatal death, 14% with erythroblastosis foetalis and 4% developed hydrops. Mild anaemia and oedema was common in primi and multigravida patients. PET was found 6.2% in multigravida patients. APH and Hydramnios with congenital anomalies were 3.1% and 3.1% respectively. This study was undertaken to evaluate the outcome of pregnancy in Rh -ve women. It is preventable. Primary prevention of isoimmunization by giving combined antenatal and postnatal prophylaxis.Medicine Today 2018 Vol.30(1): 23-25

APA, Harvard, Vancouver, ISO, and other styles

26

Flegr, Jaroslav, Manfred Milinski, Šárka Kaňková, Martin Hůla, Jana Hlaváčová, and Kateřina Sýkorová. "Latent toxoplasmosis and olfactory functions of Rh positive and Rh negative subjects." PLOS ONE 13, no. 12 (December 27, 2018): e0209773. http://dx.doi.org/10.1371/journal.pone.0209773.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Hyland, CA, LC Wolter, and A. Saul. "Three unrelated Rh D gene polymorphisms identified among blood donors with Rhesus CCee (r'r') phenotypes." Blood 84, no. 1 (July 1, 1994): 321–24. http://dx.doi.org/10.1182/blood.v84.1.321.321.

Full text

Abstract:

Abstract Human red blood cells are traditionally typed as Rhesus (Rh)-positive or -negative depending on the presence or absence of the Rh D antigen. A recent report demonstrated that the Rh D gene is completely absent in Rh D-negative individuals. In this study, Rh D-negative blood donors with ccee (n = 25) and CCee (n = 3) phenotypes were examined for the presence of absence of the D gene. Polymerase chain reaction (PCR) probes that hybridize to the 5' and 3' regions of the Rh CcEe gene and the closely related D gene were used in a Southern analysis. The D gene was absent in all ccee phenotypes examined. The CCee phenotypes showed three Rh D polymorphisms: one donor lacked the D gene, one donor had a partial deletion on one D gene at the 3' region, and the remaining donor appeared to have one normal D gene within the intron/exon regions examined. We conclude that, while the D gene may be absent in the majority of Rh D-negative phenotypes, rarer polymorphisms also occur that prevent expression of the D antigen resulting in the Rh D-negative phenotype.

APA, Harvard, Vancouver, ISO, and other styles

28

Hyland, CA, LC Wolter, and A. Saul. "Three unrelated Rh D gene polymorphisms identified among blood donors with Rhesus CCee (r'r') phenotypes." Blood 84, no. 1 (July 1, 1994): 321–24. http://dx.doi.org/10.1182/blood.v84.1.321.bloodjournal841321.

Full text

Abstract:

Human red blood cells are traditionally typed as Rhesus (Rh)-positive or -negative depending on the presence or absence of the Rh D antigen. A recent report demonstrated that the Rh D gene is completely absent in Rh D-negative individuals. In this study, Rh D-negative blood donors with ccee (n = 25) and CCee (n = 3) phenotypes were examined for the presence of absence of the D gene. Polymerase chain reaction (PCR) probes that hybridize to the 5' and 3' regions of the Rh CcEe gene and the closely related D gene were used in a Southern analysis. The D gene was absent in all ccee phenotypes examined. The CCee phenotypes showed three Rh D polymorphisms: one donor lacked the D gene, one donor had a partial deletion on one D gene at the 3' region, and the remaining donor appeared to have one normal D gene within the intron/exon regions examined. We conclude that, while the D gene may be absent in the majority of Rh D-negative phenotypes, rarer polymorphisms also occur that prevent expression of the D antigen resulting in the Rh D-negative phenotype.

APA, Harvard, Vancouver, ISO, and other styles

29

Arce, MA, ES Thompson, S. Wagner, KE Coyne, BA Ferdman, and DM Lublin. "Molecular cloning of RhD cDNA derived from a gene present in RhD- positive, but not RhD-negative individuals." Blood 82, no. 2 (July 15, 1993): 651–55. http://dx.doi.org/10.1182/blood.v82.2.651.651.

Full text

Abstract:

Abstract The Rh blood group system plays a major role in immune and nonimmune hemolytic states. Although an Rh cDNA has been previously cloned, there is no information on which Rh antigenic protein it encodes. Using polymerase chain reaction (PCR) amplification, we have identified this original Rh clone, here designated Rh21, and an additional Rh cDNA clone, Rh13, that is 96% nucleotide- and 92% amino acid-identical to Rh21, with the substitutions scattered throughout the sequence. A molecular genetic approach was used to match this Rh clone with an Rh specificity. The mRNA transcript for Rh13 was present in reticulocytes from RhD-positive individuals, but was absent from the reticulocytes of RhD-negative individuals. Using conventional screening of genomic libraries, as well as PCR cloning, partial genomic clones for these two Rh cDNAs were obtained. Based on PCR analysis and Southern blots, the Rh21 gene was present in all individuals, but an intact Rh13 gene was only present in RhD-positive and not RhD-negative individuals. Thus, by correlating the presence of Rh mRNA and gene sequences with individual Rh phenotypes, we were able to establish that the new Rh13 cDNA clone represents the RhD protein.

APA, Harvard, Vancouver, ISO, and other styles

30

Arce, MA, ES Thompson, S. Wagner, KE Coyne, BA Ferdman, and DM Lublin. "Molecular cloning of RhD cDNA derived from a gene present in RhD- positive, but not RhD-negative individuals." Blood 82, no. 2 (July 15, 1993): 651–55. http://dx.doi.org/10.1182/blood.v82.2.651.bloodjournal822651.

Full text

Abstract:

The Rh blood group system plays a major role in immune and nonimmune hemolytic states. Although an Rh cDNA has been previously cloned, there is no information on which Rh antigenic protein it encodes. Using polymerase chain reaction (PCR) amplification, we have identified this original Rh clone, here designated Rh21, and an additional Rh cDNA clone, Rh13, that is 96% nucleotide- and 92% amino acid-identical to Rh21, with the substitutions scattered throughout the sequence. A molecular genetic approach was used to match this Rh clone with an Rh specificity. The mRNA transcript for Rh13 was present in reticulocytes from RhD-positive individuals, but was absent from the reticulocytes of RhD-negative individuals. Using conventional screening of genomic libraries, as well as PCR cloning, partial genomic clones for these two Rh cDNAs were obtained. Based on PCR analysis and Southern blots, the Rh21 gene was present in all individuals, but an intact Rh13 gene was only present in RhD-positive and not RhD-negative individuals. Thus, by correlating the presence of Rh mRNA and gene sequences with individual Rh phenotypes, we were able to establish that the new Rh13 cDNA clone represents the RhD protein.

APA, Harvard, Vancouver, ISO, and other styles

31

Barty, Rebecca, Yang Liu, Menaka Pai, Anushka Jaffer, John W. Eikelboom, Richard J. Cook, and Nancy Heddle. "Utilization of Universal Group O Blood By Non Group O Recipients." Blood 124, no. 21 (December 6, 2014): 5103. http://dx.doi.org/10.1182/blood.v124.21.5103.5103.

Full text

Abstract:

Abstract INTRODUCTION: Individuals with group O blood are designated ‘universal donors’ as their red cells can be transfused to all individuals. 15% of donors are Rh negative; their red cells are particularly valuable as they are the only blood compatible with O Rh negative recipients. Canada has experienced group O blood shortages in the past, which have been aggravated by group O red cells being given to non-O recipients. We performed a study to determine the utilization patterns of group O red cells (both Rh positive and Rh negative) by non-O recipients in a large Canadian city. METHODS: Blood utilization data from all group O Rh positive and Rh negative red cells transfused at three academic hospitals between April 2002 and March 2013 were used for this study. Autologous and directed donor red cells were not included. Information was collected for all patients receiving group O blood including: age; inpatient or outpatient setting; ABO and Rh type; number of transfusions received; presence of red cell alloantibodies; most responsible diagnosis (MRD); and indications for transfusion. Descriptive statistics were performed using SAS 9.2. RESULTS: During the study period, 289,392 units of red cells were transfused, of which 139,320 were group O units. A minority of O red cells (8.7%) were transfused to non-O (A,B or AB) recipients. Of the 106,827 transfused O Rh positive red cells, 4.3% (4,594) were given to non-O individuals. Of the 32,493 transfused O Rh negative red cells, 23.2% (7,544) were given to non-O recipients. When O Rh negative red cells were considered, 32.8% (10,670) were given to Rh positive recipients. For outpatient O red cell transfusions (31,730 units), 19.6% of O Rh negative units and 5.9% of O Rh positive units were transfused to non-O recipients. For inpatient O red cell transfusions (107,590 units), 24.2% of O Rh negative units and 3.8% of O Rh positive units were transfused to non-O recipients. The most common MRD for non-O patients receiving O blood were circulatory disease, cancer, and trauma. The most common indications for transfusion of O negative blood to non-O recipients were: presence of an alloantibody requiring phenotype specific blood (26.0%); trauma requiring uncrossmatched blood (8.5%); outdating unit (12.5%); recipient was in neonatal ICU (11.5 %); and recipient was a transplant patient (6.4%). In 39.5% of cases, there was no clear indication for transfusion of O negative blood to a non-O recipient. CONCLUSIONS: Most O Rh positive blood is given to O Rh positive recipients. However, O Rh negative blood is frequently given to non-O recipients; this can contribute to shortages. A number of factors leading to transfusion of non-group identical blood - presence of an alloantibody, the need for massive/urgent transfusion (e.g., trauma), and outdating units - could be better managed by Transfusion Medicine Services to preserve the scarce resource of 'universal donor' blood. Disclosures No relevant conflicts of interest to declare.

APA, Harvard, Vancouver, ISO, and other styles

32

Rao, Chandrika, and Jayaprakash Shetty. "FREQUENCY OF ABO AND RHESUS (D) BLOOD GROUPS IN DAKSHINA KANNADA DISTRICT OF KARNATAKA - A STUDY FROM RURAL TERTIARY CARE TEACHING HOSPITAL IN SOUTH INDIA." Journal of Health and Allied Sciences NU 04, no. 03 (September 2014): 057–60. http://dx.doi.org/10.1055/s-0040-1703802.

Full text

Abstract:

Abstract Background: ABO and Rh blood groups are most important blood groups in human beings. The frequency of four main blood group systems varies in population throughout the world and even in different parts of country. Objective if this study was to identify distribution of ABO and Rh blood group system. Materials and methods: The study was conducted in rural tertiary care hospital from January 2008 to December 2012. Data were collected from Blood Bank grouping records. All blood samples processed during period of observation were included in study. Results: During the period of observation total 43,103 numbers of blood groups were performed. Patient's samples were 28,305 and donor's samples were 14,798. The frequency of blood group O in our population was 42.0% (40.1% O Rh positive and 1.8% O Rh negative). The frequency of blood group B in our population was 27.3% (25.6% B Rh positive and 1.62% B Rh negative) followed by blood group A was 25.8% (24.3% A Rh positive and 1.4% A Rh negative) and blood group AB was 4.8% (4.4% AB Rh positive and 1.4% AB Rh negative) and a two Bombay blood group donors (0.0046%). Rh positive were 94.64% and Rh negative were 5.35%. Discussion: O positive blood group is significantly high in our population. Every transfusion centre should have a record of frequency of blood group system in their population. It helps in inventory management. Knowledge of blood group distribution is important for clinical studies, for reliable geographical information and for forensic studies in the population.

APA, Harvard, Vancouver, ISO, and other styles

33

Shulman, L. P. "Do Rh-negative women with first trimester spontaneous abortions need Rh immune globulin?" Yearbook of Obstetrics, Gynecology and Women's Health 2007 (January 2007): 237. http://dx.doi.org/10.1016/s1090-798x(08)70170-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Hannafin, Blaine, Frank Lovecchio, and Paul Blackburn. "Do Rh-negative women with first trimester spontaneous abortions need Rh immune globulin?" American Journal of Emergency Medicine 24, no. 4 (July 2006): 487–89. http://dx.doi.org/10.1016/j.ajem.2006.01.020.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Szymanski, Irma O. "Rh-Positive Fresh Frozen Plasma (FFP) Therapy Can Induce Cross-Reactive Immune Response to Red Blood Cell (RBC) Self-Antigen in Rh-Negative Patients Having Existing Alloimmunization to Rh-Antigen D." Blood 124, no. 21 (December 6, 2014): 5109. http://dx.doi.org/10.1182/blood.v124.21.5109.5109.

Full text

Abstract:

Abstract Development of RBC autoantibodies in three Rh-negative patients following treatment with Rh-positive FFP is reported. Patients’ alloimmunization to Rh D antigen dates back several years. Patient I, a 49-year old man, experienced an acute cardiac event due to ascending aortic dissection, aortic insufficiency and probable cardiac tamponade. His blood type was O, Rh negative and his serum contained no alloantibodies. He underwent cardiac surgery on emergency basis and experienced bleeding complications during the procedure. He received 53 units of RBC, 49 of them Rh- positive and many other blood products. On the 8th post-operative day the patient experienced cardiac tamponade, which was relieved surgically. Then his blood type was O, Rh positive and anti-D was detected in his serum due to primary immunization to the Rh D antigen. The patient was re-admitted three months later for sharp epigastric pain. During that hospitalization no blood products were transfused; hematocrit varied between 33 to 29%. Blood typed as O, Rh negative, and manual direct antiglobulin test (DAT) was microscopically positive for IgG. Automated DAT was strongly positive for IgG1. Anti-D, anti-E and a panagglutinin (autoantibody) were present in his serum. The panagglutinin reacted strongly (2+) with Rh-positive RBC and less strongly (1+) with Rh-negative RBC, but both reactivities could be removed by absorption onto Rh-negative RBC. This “mimicking” antibody represented an autoantibody that was induced by alloimmunization to Rh antigen D. Eleven years later the patient was readmitted for bleeding duodenal ulcers. His serum contained anti-D, anti-C, anti-E and anti-K. DAT was negative for the first three days. During the first 19 days of hospitalization he received seven units of compatible RBC and 16 units of Rh-positive FFP. Anti-D was present in the eluate from patient’s Rh-negative RBC during days 18 to 28. The patient had again developed an autoantibody after receiving Rh-positive FFP, which apparently contained RBC fragments. Patient II was a 58-year old woman who underwent coronary artery bypass surgery. Her blood type was B, Rh negative and her serum contained anti-D and anti-K. During surgery and postoperative period she received ten compatible units of RBC and two units of B, Rh-positive FFP. The immediate post-operative course was unremarkable, but when discharged 8 days after surgery she had slight fever of unknown origin and Hct was 32.2%. Nine days later she was admitted to another hospital with fever of 101oF, jaundice, melena, chest pain, leg pain and anemia (Hb 7.2gm/dL). Reticulocytes were 14.8% and haptoglobin was 0. No new alloantibodies were detected. She had a weakly positive manual DAT. The automated DAT showed severe coating of RBC with IgM. Autoimmune hemolytic anemia was diagnosed. Following prednisone therapy her hemoglobin and hematocrit levels normalized. We believe that the autoantibody was induced by RBC fragments in the Rh-positive FFP. Patient III was a 72-year-old woman with admission diagnosis of possible HUS. The patients’ true blood type was A, Rh negative and her serum contained anti-C and anti-D. However, the initial blood sample was collected from a wrong patient whose blood type was O, Rh positive. Therefore she received four units of O, Rh-positive FFP before plasma exchange therapy was initiated. Her group A RBC were destroyed by anti-A in the transfused FFP. Surprisingly anti-D was also detected in the eluate of patients’ Rh-negative RBC. At that time the titer of anti-D had increased significantly over the original. Following destruction of anti-A coated RBC she still remained anemic, probably due to the autoantibody. After prednisone therapy Hct normalized steadily. In summary, three Rh-negative patients with existing alloimmunization to the Rh antigen D developed anti-D mimicking autoantibodies after receiving Rh-positive FFP. The autoantibodies caused autoimmune hemolytic anemia in two of the patients. Further studies on RBC autoimmunization in alloimmunized patients are encouraged. Disclosures No relevant conflicts of interest to declare.

APA, Harvard, Vancouver, ISO, and other styles

36

Kim, Bora. "Positive and Negative Evaluation of Texts: Based on Russian National Corpus." Institute for Russian and Altaic Studies Chungbuk University 17 (August 30, 2018): 1–18. http://dx.doi.org/10.24958/rh.2018.17.1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Suyama, K., and J. Goldstein. "Antibody produced against isolated Rh(D) polypeptide reacts with other Rh-related antigens." Blood 72, no. 5 (November 1, 1988): 1622–26. http://dx.doi.org/10.1182/blood.v72.5.1622.1622.

Full text

Abstract:

Abstract Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobinding and Western blot immunostaining assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as well as the Rh antigen-containing components of sodium dodecyl sulfate-polyacrylamide gel electrophoresis- separated membrane proteins from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde), and -D-/-D- cells. It does not react with any membrane protein from Rh-null regulator type cells, thus indicating a specificity for Rh-related proteins. We have also been able to demonstrate that polyclonal and monoclonal anti-D preparations that do not immunostain isolated Rh(D) polypeptide will react with it in our immunobinding assay.

APA, Harvard, Vancouver, ISO, and other styles

38

Suyama, K., and J. Goldstein. "Antibody produced against isolated Rh(D) polypeptide reacts with other Rh-related antigens." Blood 72, no. 5 (November 1, 1988): 1622–26. http://dx.doi.org/10.1182/blood.v72.5.1622.bloodjournal7251622.

Full text

Abstract:

Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobinding and Western blot immunostaining assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as well as the Rh antigen-containing components of sodium dodecyl sulfate-polyacrylamide gel electrophoresis- separated membrane proteins from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde), and -D-/-D- cells. It does not react with any membrane protein from Rh-null regulator type cells, thus indicating a specificity for Rh-related proteins. We have also been able to demonstrate that polyclonal and monoclonal anti-D preparations that do not immunostain isolated Rh(D) polypeptide will react with it in our immunobinding assay.

APA, Harvard, Vancouver, ISO, and other styles

39

Al Joudi, F. S., and S. A. Ahmed Al Salih. "Incidence of rhesus isoimmunization in rhesus-negative mothers in Ramadi,Iraq, in the mid-1990s." Eastern Mediterranean Health Journal 6, no. 5-6 (December 15, 2000): 1122–25. http://dx.doi.org/10.26719/2000.6.5-6.1122.

Full text

Abstract:

This work was carried out in Ramadi, Iraq over the period 1993 to 1997. Of 487 rhesus [Rh]-negative mothers tested and followed up, 172 were primigravida, 1.7% of whom were Rh-isoimmunized. The frequency of isoimmunization increased with increasing number of pregnancies [4.9% for second pregnancies to 45.4% for fifth pregnancies]. Comparison of our results with other earlier studies shows that the incidence of Rh-isoimmunization in our study was considerably greater than the others.

APA, Harvard, Vancouver, ISO, and other styles

40

Saqlain, Nazish, Aatika Ahmed, Tooba Fateen, and Nisar Ahmed. "D ANTIGEN." Professional Medical Journal 23, no. 11 (November 10, 2016): 1395–99. http://dx.doi.org/10.29309/tpmj/2016.23.11.1769.

Full text

Abstract:

Background: In 1939 Rh antigen was discovered by Levine and Stetson. Rhsystem antigens are very immunogenic, they can produce significant Hemolytic Disease of thefetus and Newborn as well as hemolytic transfusion reactions. There are numerous variants ofD, the most common subtypes are Weak D and Partial D, now called as abnormal D antigens.The incidence of Rh negativity worldwide varies between 3%-25% and that of weak D antigenranges from 0.2%-1%. Objectives: To find out the frequency of Rh negativity and weak Dantigen among the donors coming to the blood bank of The Children’s Hospital & Institute ofChild Health, Lahore and to review the clinical significance of weak D antigen in transfusionperspective especially its role in alloimmunization caused by Weak D antigen when transfusedto Rh negative individuals. Study Design: Cross- sectional study. Setting: The Children’sHospital and Institute of Child Health, Lahore. Period: 1st Jan 2015 to 31st May, 2015. Materialsand Methods: 6320 healthy donors were randomly selected. All samples were grouped forABO and Rh-D factor by immediate spin tube technique. All samples found Rh negative, werefurther processed for weak D antigen with monoclonal anti D sera by using indirect Coomb’stechnique. The presence of macroscopic or microscopic agglutination was recorded as Rhpositive. In case there was no agglutination the mixture was washed 4 times with normalsaline. After the last wash, saline was decanted and 2 drops of monoclonal, polyvalent antihuman globulin was added. Macroscopic and microscopic agglutination was looked for andany agglutination at this stage was recorded as weak D antigen. Positive control (check cellsi.e. washed O positive cells with diluted anti D) and negative control (washed O positive cells)were always put. Results: Among the 6320 healthy donors, 1224(19.4%) were Rh-D negativeand 5096(80.6%) were Rh-D positive. Of the 1224 Rh D negative samples, 3 (0.2%) samplesfound positive for weak D antigen. Conclusion: The frequency of Rh negative blood groupwas 0.2% among the healthy donors at The Children’s Hospital and ICH, Lahore. Although thefrequency is low but it’s proven by literature that weak D antigen can produce alloimmunizationif transfused to Rh-D negative subjects. At the same time the cases of hemolytic reactionsreported previously with Weak D antigen have been scarce.

APA, Harvard, Vancouver, ISO, and other styles

41

Kaminskyy, V., and L. Kovalchuk. "ASSOCIATIONS OF BLOOD GROUPS ANTIGENS OF AB0 AND RHESUS SYSTEMS WITH THE DEVELOPMENT OF CHRONIC KIDNEY DISEASE, GLOMERULONEPHRITIS." Ukrainian Journal of Nephrology and Dialysis, no. 3(43) (July 16, 2014): 35–43. http://dx.doi.org/10.31450/ukrjnd.3(43).2014.06.

Full text

Abstract:

Introduction. Finding of biological markers of genetic predisposition to the formation of glomerulonephritis (GN) will promote prediction the probability of its development still at an early stage and provide the growth of preventive direction of medicine. The purpose of the study is to evaluate the risk of GN development by antigens of AB0 and rhesus (Rh) blood groups. Materials and methods. The study included 434patients with GN(242M, 192F, aged 37.56 ± 13.01y). 1428 healthy persons was surveyed to determine the distribution of phenotypes of AB0 and Rh blood groups in the population. Results. The total value of the relative risk of GN development in all Rh–negative carriers ABprevailed by 2.34 times in the same Rh–positive. The total value of the relative risk of disease appearance in Rh–negative individuals prevailed in the same Rh–positive according to gender: in men with A and AB – 6.43 and 4.16 times, respectively, in women with B and AB – 9.34 and 2.15 times, respectively. In all patients, the common feature was a high chance of getting sick by GN in carriers phenotype AB Rh– versus 0 Rh–. Conclusions. The sex dimorphism of hereditary predisposition markers for GN is proved: men with phenotypes A Rh– and AB Rh–, women with B Rh–, AB Rh– and AB Rh+ have high risk to be ill. The persons of both sexes with phenotype 0 Rh–, as well as men with B Rh– and women with A Rh– and B Rh+ may be resistant to disease.

APA, Harvard, Vancouver, ISO, and other styles

42

Odata, Sana’a, Abu Allabanb, and Khitam Odibatb. "Influence of Meteorological Parameters on Air Quality at Hashemite University, Jordan." Current World Environment 12, no. 2 (August 25, 2017): 211–21. http://dx.doi.org/10.12944/cwe.12.2.04.

Full text

Abstract:

Four threshold air pollutants (SO2, NO, NO2, and O3) in addition to meteorological parameters were monitored at the Campus of the Hashemite University (HU) for two years (1/1/2012 through 30/12/013). Correlations between air pollution and meteorological parameters were derived. The results showed that O3 has a positive correlation with air temperature, wind speed and wind direction, but has a negative correlation with the relative humidity (RH). SO2 was found to have a negative correlation with the RH and wind speed, but positive correlation with air temperature. NO has negative correlation with air temperature, RH, and wind speed. And finally, NO2 has a negative correlation with RH and wind speed, but it has positive correlation with air temperature. Justify the reasons in brief with recommendations to improve the air quality

APA, Harvard, Vancouver, ISO, and other styles

43

Sostin, N., J. Hendrickson, R. Balbuena-Merle, and C. Tormey. "Passive Transfer Of Allo-Antibodies Following Rhig Administration Given For Rh-Mismatched Platelets Prophylaxis." American Journal of Clinical Pathology 154, Supplement_1 (October 2020): S158—S159. http://dx.doi.org/10.1093/ajcp/aqaa161.346.

Full text

Abstract:

Abstract Introduction/Objective Human platelets (PLTs) do not express any Rh system antigens; however, PLT concentrates can be contaminated with small amounts of red blood cells (RBCs), which may induce alloimmunization when transfused to Rh(D)-negative individuals. RhIG has been utilized to prevent Rh(D) alloantibody development following transfusion of Rh mismatched PLTs. RhIG is manufactured using pooled plasma of healthy Rh(D)-negative donors, with the most common Rh haplotype being ce; treated subjects are exposed to Rh (D)-positive RBCs, with the most common Rh haplotype of donors being DCe. In this report, we detail our experiences with recipients of Rh mismatched apheresis PLTs who were noted to develop anti-D + anti-C post-RhIG administration. Methods Retrospective review was conducted of all Rh mismatched PLTs between December, 2018 and May, 2019 at our facility (Yale-New Haven Hospital, New Haven, CT). Inclusion in the study required: Rh(D)-negative donor receiving one or more Rh(D)-positive apheresis PLTs, Receiving RhIG, >1 antibody screen following RhIG administration demonstrating antibodies other than anti-D Results Our retrospective review identified 8 unique recipients of Rh mismatched apheresis PLTs who acquired anti- C, in addition to the expected anti-D, following administration of RhIG. The product (Rhophylac) was administered in all cases intravenously at a dose of 1500 IU (300 mcg) within 72 hours following Rh mismatched PLTs. In all patients, routine screening following RhIG simultaneously detected anti-D and anti-C 1-3 days after administration of Rh mismatched PLTs/RhIG, the antibody screen remained positive for a range of 27-167 days for both antibodies. Conclusion Based on this case series, which represented entirely men and older women, and coupled with emerging evidence about the extremely low likelihood of D-alloimmunization following Rh mismatched apheresis PLTs,2,3,6 we have changed our practice, limiting immunoprophylaxis for Rh mismatched platelets exclusively to women of reproductive age. The blood bank and apheresis communities should be aware that passive transfer of non-D antibodies is possible when RhIG is dosed and could account for newly-detected non-D alloimmunization events. This phenomenon is another compelling reason to limit RhIG exposure to cases where it is only absolutely clinically necessary.7

APA, Harvard, Vancouver, ISO, and other styles

44

Pasha, R. P. K., M. A. Shokrgozar, Z. S. Bahrami, and F. Shokri. "Frequency analysis of B lymphocytes specific for Rh antigens in naturally immunized Rh-negative women." Vox Sanguinis 86, no. 1 (January 2004): 62–70. http://dx.doi.org/10.1111/j.0042-9007.2004.00378.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Saboori, A. M., B. L. Smith, and P. Agre. "Polymorphism in the Mr 32,000 Rh protein purified from Rh(D)-positive and -negative erythrocytes." Proceedings of the National Academy of Sciences 85, no. 11 (June 1, 1988): 4042–45. http://dx.doi.org/10.1073/pnas.85.11.4042.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Covali, Roxana, Demetra Socolov, Ioana Pavaleanu, Alexandru Carauleanu, Vasile Lucian Boiculese, and Razvan Socolov. "SARS-CoV-2 Infection Susceptibility of Pregnant Patients at Term Regarding ABO and Rh Blood Groups: A Cohort Study." Medicina 57, no. 5 (May 14, 2021): 499. http://dx.doi.org/10.3390/medicina57050499.

Full text

Abstract:

Background and Objectives: The susceptibility of pregnant patients at term to SARS-CoV-2 infection regarding the ABO and Rh blood group polymorphism was analyzed in this study. Materials and Methods: In this prospective study, 457 patients admitted for delivery at term in our hospital, between 1 April 2020 and 31 December 2020 were studied. There were 46 positive and 411 SARS-CoV-2 negative patients. Their values for RT-PCR, ABO, and Rh blood group analyses, which were determined upon admittance, were studied. Results: A slightly higher percentage of infected pregnant patients at term belonged to the A blood group compared with the percentage belonging to the other blood groups; this was also true for the healthy control group. For the Rh-negative pregnant patients at term, the odds of being infected with SARS-CoV-2 was OR = 1.22 compared with Rh-positive patients where OR = 1. In our study, the highest risk was found among BIII Rh-negative pregnant patients at term (OR = 3). None of the above differences were statistically significant. Conclusions: No significant difference was found between the percentage of ABO or Rh blood groups in SARS-CoV-2 positive patients when compared with SARS-CoV-2 negative patients (p = 0.562).

APA, Harvard, Vancouver, ISO, and other styles

47

Turaev, R. G., and E. E. Bel’skaya. "The rationale for the post-transfusion reactions prevention program in rh-positive patients." Kazan medical journal 94, no. 5 (October 15, 2013): 764–65. http://dx.doi.org/10.17816/kmj1939.

Full text

Abstract:

A case of a patient with А 2В (IV) Rh 0(D) + blood group, CcDEeK - phenotype, in whom anti-erythrocyte antibodies (+) were found and specified as anti-D antibodies, is presented. The Rh compatibility tests were positive with 28% Rh 0(D) +-donors (incompatibility) and negative with all Rh 0(D) --donors (compatibility) as well as with own erythrocytes (compatibility). The case indicates that a timely extensive examination of a patient’s blood (antigen profile examination with anti-erythrocyte antibodies detection) before blood transfusion guarantees the blood transfusion safety and reduces the rate of negative consequences of erythrocyte-containing blood components, including hemolytic reactions.

APA, Harvard, Vancouver, ISO, and other styles

48

Simsek, S., BH Faas, PM Bleeker, MA Overbeeke, HT Cuijpers, CE van der Schoot, and AE von dem Borne. "Rapid Rh D genotyping by polymerase chain reaction-based amplification of DNA." Blood 85, no. 10 (May 15, 1995): 2975–80. http://dx.doi.org/10.1182/blood.v85.10.2975.bloodjournal85102975.

Full text

Abstract:

Rh (rhesus) D is the dominant antigen of the Rh blood group system. Recent advances in characterization of the nucleotide sequence of the cDNA(s) encoding the Rh D polypeptide allow the determination of the Rh D genotype at the DNA level. This can be of help in cases in which red blood cells are not available for phenotyping, eg, when in concerns a fetus. We have tested three independent DNA typing methods based on the polymerase chain reaction (PCR) for their suitability to determine the Rh D genotype. DNA derived from peripheral blood mononuclear cells from 234 Rh-phenotyped healthy donors (178 Rh D positive and 56 Rh D negative) was used in the PCR. The Rh D genotypes, as determined with a method based on the allele-specific amplification of the 3′ noncoding region of the Rh D gene described by Bennett et al (N Engl J Med 329:607, 1993), were not concordant with the serologically established phenotypes in all cases. We have encountered 5 discrepant results, ie, 3 false-positive and 2 false-negative (a father and child). Rh D genotyping with the second method was performed by PCR amplification of exon 7 of the D gene with allele-specific primers. In all donors phenotyped as D positive tested so far (n = 178), the results of molecular genotyping with this method were concordant with the serologic results, whereas a false-positive result was obtained in one of the D-negative donors (also false-positive in the first method). Complete agreement was found between genotypes determined in the third method, based on a 600-bp deletion in intron 4 of the Rh D gene described by Arce et al (Blood 82:651, 1993), and serologically determined phenotypes. The Rh blood group system is complex, and unknown polymorphisms at the DNA level are expected to exist. Therefore, although genotypes determined by the method of Arce et al were in agreement with serotypes, it cannot yet be regarded as the golden standard. More experience with this or other methods is still needed.

APA, Harvard, Vancouver, ISO, and other styles

49

Junaid, Nadira, Julie Fung, Nadine Shehata, and Marietta Biscocho. "Is 120μg Rh Immune Globulin sufficient for prophylactic treatment of postpartum Rh(D) negative women delivering an Rh(D) positive neonate?" Transfusion Medicine Reviews 35, no. 1 (January 2021): 57. http://dx.doi.org/10.1016/j.tmrv.2020.09.015.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Shao, Huiqi, Yifan Ren, Yan Zhang, Chuandong Wu, Wenhui Li, and Jiemin Liu. "Factor analysis of the influence of environmental conditions on VOC emissions from medium density fibreboard and the correlation of the factors with fitting parameters." RSC Advances 11, no. 42 (2021): 26151–59. http://dx.doi.org/10.1039/d1ra02164h.

Full text

Abstract:

RH has positive effects on the initial VOC emissions and ACR has negative effects on VOC emissions. a₁ has a power relationship with ACR and a polynomial relationship with RH and b₁ has a polynomial relationship with both ACR and RH.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Rh negativo'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles