Relevant bibliographies by topics / Rhizobium japonicum

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Academic literature on the topic 'Rhizobium japonicum'

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Published: 4 June 2021
Last updated: 25 January 2023

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Journal articles on the topic "Rhizobium japonicum"

1

Enibukun, Jesupemi Mercy, and Bolatito Esther Boboye. "Molecular characterization and evaluation of crude oil remediation potential of some rhizobia isolated from plant root nodules." Nova Biotechnologica et chimica 19, no. 1 (June 30, 2020): 80–88. http://dx.doi.org/10.36547/nbc.v19i1.580.

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This study aimed to determine the molecular identities and genetic relatedness of rhizobia isolated from pigeon pea and pinto beans, and assess their remediation potential in the presence of 1 %, 3 % and 5 % (w/v) crude oil in minimal medium for 7 days incubation period. Standard microbiological and molecular methods which include amplification and purification of 16S rRNA, agarose gel electrophoresis, and sequencing. Results showed molecular identities of six rhizobia from pigeon peas as Bradyrhizobium diazoefficiens USDA122, Rhizobium leguminosarum WSM2304, Bradyrhizobium japonicum N61, Rhizobium leguminosarum N741, Rhizobium leguminosarum BIHIB1217, and Bradyrhizobium japonicum E109; and three rhizobia obtained from pinto beans were Rhizobium leguminosarum N871, Bradyrhizobium diazoefficiens USDA110 and Bradyrhizobium japonicum SEMIA5079. All tested rhizobia (9) showed petroleum degradation ability, as they all grew in the 1, 3 and 5 % (w/v) crude oil minimal medium under laboratory conditions. B. diazoefficiens USDA122 showed the highest optical density (OD) value of 1.184 ± 0.05 on 7th day at 1 % (w/v) crude oil contamination, while R. leguminosarum N741 has the lowest OD value of 0.372 ± 0.02 at 5 % (w/v) crude oil on 7th day. For all the rhizobia, increase occurred throughout incubation period at 1, 3 and 5 % (w/v) except Rhizobium leguminosarum N741 and R. leguminosarum BIHIB1217. In conclusion, the association of R. leguminosarum BIHIB1217 and R. leguminosarum N871 from pigeon pea and pinto beans respectively, were found most effective in crude oil degradation and thus they are recommended as a promising association for remediation of crude oil spilled soils.
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Castellano-Hinojosa, Antonio, Christoph Mora, and Sarah L. Strauss. "Native Rhizobia Improve Plant Growth, Fix N2, and Reduce Greenhouse Emissions of Sunnhemp More than Commercial Rhizobia Inoculants in Florida Citrus Orchards." Plants 11, no. 22 (November 8, 2022): 3011. http://dx.doi.org/10.3390/plants11223011.

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Sunnhemp (Crotalaria juncea L.) is an important legume cover crop used in tree cropping systems, where there is increased interest by growers to identify rhizobia to maximize soil nitrogen (N) inputs. We aimed to isolate and identify native rhizobia and compare their capabilities with non-native rhizobia from commercial inoculants to fix atmospheric dinitrogen (N2), produce and reduce nitrous oxide (N2O), and improve plant growth. Phylogenetic analyses of sequences of the 16S rRNA and recA, atpD, and glnII genes showed native rhizobial strains belonged to Rhizobium tropici and the non-native strain to Bradyrhizobium japonicum. Plant nodulation tests, sequencing of nodC and nifH genes, and the acetylene-dependent ethylene production assay confirmed the capacity of all strains to nodulate sunnhemp and fix N2. Inoculation with native rhizobial strains resulted in significant increases in root and shoot weight and total C and N contents in the shoots, and showed greater N2-fixation rates and lower emissions of N2O compared to the non-native rhizobium. Our results suggest that native rhizobia improve plant growth, fix N2, and reduce greenhouse emissions of sunnhemp more than commercial rhizobia inoculants in Florida citrus orchards.
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Boncompagni, Eric, Magne Østerås, Marie-Christine Poggi, and Daniel le Rudulier. "Occurrence of Choline and Glycine Betaine Uptake and Metabolism in the Family Rhizobiaceae and Their Roles in Osmoprotection." Applied and Environmental Microbiology 65, no. 5 (May 1, 1999): 2072–77. http://dx.doi.org/10.1128/aem.65.5.2072-2077.1999.

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ABSTRACT The role of glycine betaine and choline in osmoprotection of various Rhizobium, Sinorhizobium,Mesorhizobium, Agrobacterium, andBradyrhizobium reference strains which display a large variation in salt tolerance was investigated. When externally provided, both compounds enhanced the growth of Rhizobium tropici,Sinorhizobium meliloti, Sinorhizobium fredii,Rhizobium galegae, Agrobacterium tumefaciens,Mesorhizobium loti, and Mesorhizobium huakuii, demonstrating their utilization as osmoprotectants. However, both compounds were inefficient for the most salt-sensitive strains, such asRhizobium leguminosarum (all biovars), Agrobacterium rhizogenes, Rhizobium etli, and Bradyrhizobium japonicum. Except for B. japonicum, all strains exhibit transport activity for glycine betaine and choline. When the medium osmolarity was raised, choline uptake activity was inhibited, whereas glycine betaine uptake was either increased in R. leguminosarum and S. meliloti or, more surprisingly, reduced in R. tropici, S. fredii, and M. loti. The transport of glycine betaine was increased by growing the cells in the presence of the substrate. With the exception ofB. japonicum, all strains were able to use glycine betaine and choline as sole carbon and nitrogen sources. This catabolic function, reported for only a few soil bacteria, could increase competitiveness of rhizobial species in the rhizosphere. Choline dehydrogenase and betaine-aldehyde dehydrogenase activities were present in the cells of all strains with the exception of M. huakuii and B. japonicum. The main physiological role of glycine betaine in the family Rhizobiaceae seems to be as an energy source, while its contribution to osmoprotection is restricted to certain strains.
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Werner, Dietrich, Robert B. Mellor, Michael G. Hahn, and Hans Grisebach. "Soybean Root Response to Symbiotic Infection Glyceollin I Accumulation in an Ineffective Type of Soybean Nodules with an Early Loss of the Peribacteroid Membrane." Zeitschrift für Naturforschung C 40, no. 3-4 (April 1, 1985): 179–81. http://dx.doi.org/10.1515/znc-1985-3-407.

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A glyceollin I accumulation of about 6000 pmol · mg dry weight-, a tenfold increase above control root tissue, was found in one type of nodule from Glycine max which had been infected with a fix- strain (61-A-24) of Rhizobium japonicum. In nodules infected with one other ineffective (fix-) strain of Rhizobium japonicum (RH 31-Marburg) or with two fix+ strains of Rhizobium japonicum (61-A-101 and USD A 110) no increase in glyceollin I concentrations above control values was found at either 20 d or 34 d after infection. Nodules infected with Rhizobium japonicum 61-A-24 are distinguished by an early loss of the peribacteroid membrane in the infected host cell, whilst the bacteroids themselves remain stable.
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Herridge, DF, RJ Roughley, and J. Brockwell. "Low survival of Rhizobium japonicum inoculant leads to reduced nodulation, nitrogen fixation and yield of soybean in the current crop but not in the subsequent crop." Australian Journal of Agricultural Research 38, no. 1 (1987): 75. http://dx.doi.org/10.1071/ar9870075.

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Reports of nodulation failures in first-year crops of soybean, resulting in nitrogen deficiency and low yield, have been common in Australia. In experiments simulating commercial practice, successive crops (1982-83, 1983-84) of soybean were sown into soil containing either a very small population of Rhizobium japonicum (rhizobia-low; 2 g-1 soil, 3-9 cm), or a large population (rhizobia-high; 270 000 g-1). The soil was a vertisol at Breeza, N.S.W. Each crop was inoculated with R. japonicum at a normal rate (n = 25 x 1010 organisms per 100 kg of seed) or at 100 x normal (100n) or was not inoculated (o). The 1982-83 crop was sown in hot (maximum air temperature 3g�C), dry weather, and < 1% of the inoculant survived the first day. This did not affect nodulation in rhizobia-high plots and production of foliage and seed nitrogen was satisfactory, irrespective of rate of inoculation. In contrast, nodulation was inadequate in all rhizobia-low soil (although 100n > n > o) and the N content of foliage and seed was correspondingly reduced. In spite of poor nodulation in these rhizobia-low treatments, a substantial population of R. japonicum (18 000 g-1soil) developed between seasons. When the plots were re-sown in 1983-84, nodulation, growth and yield of the crops, irrespective of the initial rhizobial status of the soil, were equally good, and there was no response to further inoculation. Poor nodulation of first-year crops of soybean is a likely consequence of failure to establish adequate numbers of R. japonicum in the soil at sowing.
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Baginsky, Cecilia, Belén Brito, Juan Imperial, José-Manuel Palacios, and Tomás Ruiz-Argüeso. "Diversity and Evolution of Hydrogenase Systems in Rhizobia." Applied and Environmental Microbiology 68, no. 10 (October 2002): 4915–24. http://dx.doi.org/10.1128/aem.68.10.4915-4924.2002.

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ABSTRACT Uptake hydrogenases allow rhizobia to recycle the hydrogen generated in the nitrogen fixation process within the legume nodule. Hydrogenase (hup) systems in Bradyrhizobium japonicum and Rhizobium leguminosarum bv. viciae show highly conserved sequence and gene organization, but important differences exist in regulation and in the presence of specific genes. We have undertaken the characterization of hup gene clusters from Bradyrhizobium sp. (Lupinus), Bradyrhizobium sp. (Vigna), and Rhizobium tropici and Azorhizobium caulinodans strains with the aim of defining the extent of diversity in hup gene composition and regulation in endosymbiotic bacteria. Genomic DNA hybridizations using hupS, hupE, hupUV, hypB, and hoxA probes showed a diversity of intraspecific hup profiles within Bradyrhizobium sp. (Lupinus) and Bradyrhizobium sp. (Vigna) strains and homogeneous intraspecific patterns within R. tropici and A. caulinodans strains. The analysis also revealed differences regarding the possession of hydrogenase regulatory genes. Phylogenetic analyses using partial sequences of hupS and hupL clustered R. leguminosarum and R. tropici hup sequences together with those from B. japonicum and Bradyrhizobium sp. (Lupinus) strains, suggesting a common origin. In contrast, Bradyrhizobium sp. (Vigna) hup sequences diverged from the rest of rhizobial sequences, which might indicate that those organisms have evolved independently and possibly have acquired the sequences by horizontal transfer from an unidentified source.
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Nagata, Maki, Ei-ichi Murakami, Yoshikazu Shimoda, Fuyuko Shimoda-Sasakura, Ken-ichi Kucho, Akihiro Suzuki, Mikiko Abe, Shiro Higashi, and Toshiki Uchiumi. "Expression of a Class 1 Hemoglobin Gene and Production of Nitric Oxide in Response to Symbiotic and Pathogenic Bacteria in Lotus japonicus." Molecular Plant-Microbe Interactions® 21, no. 9 (September 2008): 1175–83. http://dx.doi.org/10.1094/mpmi-21-9-1175.

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Symbiotic nitrogen fixation by the collaboration between leguminous plants and rhizobia is an important system in the global nitrogen cycle, and some molecular aspects during the early stage of host-symbiont recognition have been revealed. To understand the responses of a host plant against various bacteria, we examined expression of hemoglobin (Hb) genes and production of nitric oxide (NO) in Lotus japonicus after inoculation with rhizobia or plant pathogens. When the symbiotic rhizobium Mesorhizobium loti was inoculated, expression of LjHb1 and NO production were induced transiently in the roots at 4 h after inoculation. In contrast, inoculation with the nonsymbiotic rhizobia Sinorhizobium meliloti and Bradyrhizobium japonicum induced neither expression of LjHb1 nor NO production. When L. japonicus was inoculated with plant pathogens (Ralstonia solanacearum or Pseudomonas syringae), continuous NO production was observed in roots but induction of LjHb1 did not occur. These results suggest that modulation of NO levels and expression of class 1 Hb are involved in the establishment of the symbiosis.
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Bhagwat, Arvind A., and Donald L. Keister. "Synthesis of β-glucans by Bradyrhizobium japonicum and Rhizobium fredii." Canadian Journal of Microbiology 38, no. 6 (June 1, 1992): 510–14. http://dx.doi.org/10.1139/m92-084.

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Particulate enzyme preparations from Rhizobium and Bradyrhizobium synthesize β-glucans when incubated with uridine diphosphate glucose (UDP-glucose) and a divalent cation. Synthesis of β-1,2-linked glucans in Rhizobium fredii involves the product of the ndvB gene, a 319-kDa membrane protein, which is labeled with [14C]glucose from UDP-[14C]glucose as previously demonstrated in Rhizobium meliloti and Agrobacterium sp. Bradyrhizobium japonicum synthesize β-1,3- and β-1,6-linked glucans of a lower molecular weight than those synthesized by R. meliloti. In comparative experiments, no evidence was found for a protein-bound intermediate in B. japonicum. The ndvB gene of R. fredii was mobilized to B. japonicum and the gene was expressed, as evidenced by appearance of a large membrane protein (ca. 319 kDa) which was labeled with UDP-[14C]glucose in vitro. Key words: soybean, nitrogen fixation, β-glucan, Bradyrhizobium, Rhizobium.
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Kameneva, I. O. "THE GROWTH OF BRADYRHIZOBIUM JAPONICUM AT CULTIVATION IN THE GEL MEDIUM." Agriciltural microbiology 13 (August 19, 2011): 108–16. http://dx.doi.org/10.35868/1997-3004.13.108-116.

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The influence of exopolysaccharideacrylamide (EPAA) in a liquid medium on the growth, retaining of viability and virulence of soybean rhizobia under the periodic cultivation conditions was investigated. It was observed that maximum titer of B. japonicum M-8 (B-7198) and B. japonicum 36 (B-1798) at thickening of medium has increased by 31.4% and 79.4% respectively. In the phase of exponential growth the upward trend in growth activity of rhizobia in the gel medium was observed. It was shown that the B. japonicum 36 is more sensitive than B. japonicum M-8 to the changes in the medium composition and the cultivation conditions. Absolute speed of B. japonicum 36 growth has increased in 5 times while the generation time has decreased in almost 3 times in comparison to the liquid medium. The viability and virulence of both rhizobium strains have remained over five months. It was established that the EPAA is a technological agent for the medium thickening and stabilization of the created on the basis of the soybean nodule bacteria gel preparation.
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Purwaningsih, Okti, C. Tri Kusumastuti, Y. Sulistyo Nugroho, and Casper Yoda Morib. "The Effect of Rhizobium japonicum on the Growth of Soybean Cultivars in Coastal Area." Ilmu Pertanian (Agricultural Science) 4, no. 1 (May 29, 2019): 33. http://dx.doi.org/10.22146/ipas.36371.

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The natural resources of the coastal area in Indonesia have the potential to be developed as an agricultural land with the support of both cultivation technology and land processing, which one of them was done by using Rhizobium japonicum bacteria to meet the need of nitrogen in the soybean plants. This study aimed to determine the characteristics of nitrogen fixation in various soybean cultivars planted in the coastal area. The research was conducted in Mancingan, Parangtritis, Kretek, Bantul, DIY. The study was designed in Completely Randomized Design which consisted of two factors and was repeated three times. Factor I was Rhizobium japonicum inoculation (with inoculation and without inoculation); factor II was 10 various cultivars of soybean (Grobogan, Burangrang, Argomulyo, Anjasmara, Dena 1, Gema, Kaba, Wilis, Sinabung, Gepak Kuning). The results showed that the inoculation of Rhizobium japonicum in soybean cultivars in a coastal area could increase the number of root nodule, dry weight of root nodule, dry seed weight per plant, and harvest index. Burangrang cultivar planted in the coastal area was the most responsive to Rhizobium japonicum inoculation among other soybean cultivars tested.
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Dissertations / Theses on the topic "Rhizobium japonicum"

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Payne, John Howard. "Interaction of Rhizobium japonicum with soybean isolines carrying unique genes which affect nodulation at the Rj1 locus." Gainesville, FL, 1985. http://www.archive.org/details/interactionofrhi00payn.

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Giannakis, Christos. "Nitrate utilization by cultured cells and bacteroids of Bradyrhizobium japonicum /." Title page, contents and summary only, 1987. http://web4.library.adelaide.edu.au/theses/09A/09ag433.pdf.

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Sista, Prakash Rao. "Characterization of nodulation defective mutants of Bradyrhizobium japonicum." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75665.

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The Rhizobium-legume symbiosis is an opportunistic association between two symbiotic partners that results in the formation of the root nodule. The process depends on the expression of a number of plant and bacterial genes that are considered critical for the establishment and maintainance of the symbiotic state. The merits of a mutational approach to the analysis of symbiosis have been recognized for several years and transposon Tn5 mutagenesis of Rhizobium has led to the identification of several symbiotic genes. This study describes the use of Tn5 mutagenesis for the isolation of symbiotically defective mutants of Bradyrhizobium japonicum. Two classes, auxotrophic and cell surface-altered mutants defective in nodule formation, have been characterized. In B. japonicum USDA 122, histidine auxotrophs that are defective in nodulation have been studied. The mutagenized DNA region has been cloned and the wild-type DNA region isolated by hybridization and complementation. In B. japonicum 61A76, Tn5-induced cell surface-altered mutants have been isolated by selecting for bacteriophage resistance. Several parameters have been used to demonstrate alterations in cell surface components. It has been shown that the Tn5 insertion is not the primary cause of the mutation in two of the characterized mutants. Complementation tests have led to the isolation of a wild-type DNA-containing cosmid, pPS23A, that overcomes the symbiotic defect in one of the mutants. Analysis of the cell surface showed a partial restoration of surface components in the complemented mutant.
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Kaluza, Klaus. "Strukturanalyse des NIFDK Operons und repetitiver Sequenzen von Rhizobium japonicum /." [S.l.] : [s.n.], 1985. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=7831.

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Fantauzzo, Françoise. "Etude des évènements précoces de l'interaction "cellules de soja/Bradyrhizobium japonicum" dans un système in vitro." Aix-Marseille 2, 1988. http://www.theses.fr/1988AIX22072.

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En co-culture de cellules de soja et de b. Japonicum, tous deux en croissance exponentielle, il apparait que des cultures de cellules de soja indifferenciees contiennent des substances capables d'activer specifiquement la capacite des b. Japonicum a stimuler la croissance et la synthese proteique des cellules de soja. On etudie les echanges moleculaires et leurs effets sur les deux partenaires de la symbiose
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D'Aoust, Frédéric. "Characterization of the nod and sdh operons in the legume symbionts Bradyrhizobium japonicum and Sinorhizobium meliloti." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100341.

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This study was undertaken to characterize the nod and sdh operons of Bradyrhizobium japonicum and Sinorhizobium meliloti. Ten putative B. japonicum mutants with altered nod gene induction characteristics were isolated by screening mutants for genistein-independent nod gene expression. The mutants were found to have higher nodY expression than the wild-type in the presence of genistein. The increased sensitivity of all mutants to genistein was more apparent under suboptimal inducer concentration (0.1muM) and/or temperature (15°C). The expression of nodY gene induction was determined for five strains (Bj30050, 53, 56, 57, 58) under different temperature and inducer conditions. These five strains were also found to produce more lipochitooligosaccharide than the wild-type, at both 25°C and 15°C. Three of the ten mutant strains (including Bj30056 and 57) were unable to fix nitrogen with soybeans grown at optimal temperatures. Based on nodY gene expression and symbiotic phenotype the B. japonicum mutants were classified into three groups.
A molecular genetic approach was taken to investigate the regulation of expression of succinate dehydrogenase (SDH) in S. meliloti. The sdhCDAB genes encoding SDH were shown by RT-PCR to be co-transcribed and thus constitute an operon. The transcriptional start site and putative promoter region of the first gene in the operon, sdhC , were identified by 5'-RACE and DNA sequence analysis. Transcriptional lacZ fusions to sdhC indicated that expression of the operon is regulated by carbon source in the growth medium but not by growth phase. The highest expression of the sdh operon was observed in cells grown with acetate, arabinose and glutamate, as sole carbon sources, and the lowest expression was observed in cells grown with glucose and pyruvate as sole carbon sources.
Also presented is the isolation and characterization of the first defined sdh mutant in a rhizobial species. The mutants helped demonstrate that the total lack of SDH activity would be lethal to S. meliloti cells. Symbiotic phenotype of the mutants indicated that SDH is required for N2-fixation.
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Thieme, Sebastian. "Entwurf und Verwendung eines Microarrays zur Untersuchung des Genistein-Stimulons bei Bradyrhizobium japonicum." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1191827101651-16987.

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Das Bakterium Bradyrhizobium japonicum ist wie andere Rhizobien in der Lage mit Pflanzen der Familie Fabales (Leguminosen) eine Symbiose einzugehen. Im symbiontischen Zustand fixieren die Mikrosymbionten atmosphärischen, molekularen Stickstoff und stellen diesen den Pflanzen in verwertbarer Form zur Verfügung. Im Gegenzug erhalten die Bakterien von den Pflanzen verschiedene Verbindungen als Kohlenstoff- und Energiequelle. Dem geht ein komplexer Signalaustausch voraus um die gegenseitige Erkennung der Partner und die Spezies-spezifische Symbiose zu ermöglichen. Auf Seite der Bakterien erfolgt die Reaktion auf die Gegenwart pflanzlicher Signalmoleküle. In B. japonicum induziert das Flavonoid Genistein die Transkription einer Reihe von Genen. Durch die Expression der nod-Gene erfolgt eine Synthese von Lipochitooligosacchariden. Diese sogenannten Nodulationsfaktoren rufen wiederum eine Reaktion in der Wirtspflanze hervor. Um die zugrunde liegende Genexpression und deren Regulationsmechanismen zu erforschen, standen bis dato nur Methoden für die Untersuchung einzelner Gene zur Verfügung. Die erstmals 1995 publizierte Microarraytechnologie eröffnete die Möglichkeit zu einem Zeitpunkt die Gentranskription eines gesamten Organismus zu untersuchen (Schena et al. 1995). Um mit dieser Technologie die Gentranskription bei B. japonicum zu untersuchen, wurde ein auf PCR-Produkten basierender Microarray hergestellt. Grundlage für die Synthese genspezifischer PCR-Produkte war die symbiontische Region von B. japonicum und andere zu diesem Zeitpunkt bekannte Gensequenzen (Göttfert et al. 2001). Nach der 2002 erfolgten Veröffentlichung des Genoms von B. japonicum erfolgte ein Abgleich der bisher verwendeten Sequenzen mit der vollständigen Sequenzinformation (Kaneko et al. 2002a). Nach Synthese der PCR-Produkte und deren Kontrolle durch Sequenzierung erfolgte die Herstellung des Microarrays unter Einsatz eines Microarrayspotters. Geeignete Techniken der cDNA-Markierung und die Microarray-Hybridisierung wurden mit Total-RNA von B. japonicum-Kulturen ausgetestet und etabliert. Eine differentielle Expremierung war eine Stunde nach Genistein- bzw. Methanolgabe zum Kulturmedium nicht nachweisbar. Zum darauffolgenden Zeitpunkt konnte die bekannte Induktion der nod-Gene durch dass Flavonoid Genistein beobachtet werden. Diese Induktion fiel in den verbleibenden zwei Zeitpunkten stark ab. Dieser Abfall der Induktionsrate ließ sich nicht mit dem Genisteingehalt im Kulturmedium erklären, da dieser Zeitraum konstant blieb. Vier Stunden nach Genisteingabe war die Induktion der Gene nolA und nodD2, deren Produkte sind an der negativen Regulation der nod-Gene beteiligt, nachweisbar. Dies wäre eine mögliche Erklärung für den Abfall der Induktion der nod-Gene. Im gleichen untersuchten Zeitraum wurde die Transkription verschiedener Gene der Stickstofffixierung und Denitrifikation durch das Flavonoid Genistein induziert. Auch für die bekannten Regulatoren dieser Gene war eine Induktion nachweisbar. Nach bisherigen Arbeiten war die Expression dieser Gene auf mikroaerobe und anaerobe Zustände, wie z.B. dem symbiontischen Stadium, beschränkt. Eine Induktion durch Flavonoide ist bisher nicht beobachtet worden. Um die Verwendbarkeit des Microarrays auch für den symbiontischen Zustand von B. japonicum zu testen, wurden Versuche mit Wurzelknöllchen von Sojabohne (Glycine max) durchgeführt. Dafür wurden Keimlinge der Sojabohne (G. max) mit B. japonicum inokuliert und die Total-RNA der entstehenden Wurzelknöllchen isoliert. Jedoch war eine Kreuzhybridisierung mit pflanzlicher Total-RNA zu beobachten. Der auf PCR-Produkten basierende Microarray ist für die Untersuchung des symbiontischen Stadiums von B. japonicum nicht einsetzbar. Im Vergleich dazu wurde ein auf Oligomeren basierender, kommerzieller Microarray für diesen Versuch getestet. Die Kreuzhybridisierung mit pflanzlicher Total-RNA war auf die Oligomere für die 16S rDNA und 23S rDNA reduziert.
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Belkheir, Ali Mohamed. "Variability among soybean [Glycine max (L.) Merr.] cultivars in response to genistein pre-incubated (Brady)rhizobium japonicum." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ55037.pdf.

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Alcantar, Gonzalez Gabriel. "Contribution à l'étude de l'action inhibitrice du nitrate sur la symbiose Soja-Rhizobium Japonicum : relation avec l'activité nitrate réductase des Bactéroïdes." Paris 11, 1987. http://www.theses.fr/1987PA112147.

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La relation entre le nitrate réductase inductible des bactéroïdes et le degré d'inhibition de la fixation de l'azote atmosphérique par le nitrate est étudiée dans la symbiose du Soja avec Rhizobium japonicum à nitrate réductase inductible (NR) ou dépourvu de nitrate réductase inductible (NR-). L'efficacité des symbioses exprimée par la croissance des plantes et la quantité d’azote atmosphérique fixé est comparable. Une plus grande sensibilité de l’ARA à l'effet toxique du nitrate, est associée à la présence d'un nitrate réductase inductible chez les bactéroïdes. Les "effets directs" liés à la présence de l'ion nitrate dans les nodosités sont distingués des "effets indirects" conséquences dans les nodosités du métabolisme du nitrate dans les tissus de la plante hôte. Une diminution de la charge énergétique "moyenne des nodosités" est observée dans les nodosités à R. Japonicum NR+ ce qui contraste avec l'augmentation de la charge énergétique des nodosités à R. Japonicum NR-. La mise en place de la nitrate réductase inductible serait donc consommatrice d'énergie aux dépends du fonctionnement de l'ARA. Un effet dépressif du nitrate sur l'activité phosphoénolpyruvate carboxylase des nodosités à R. Japonicum NR+ est montré. Cet effet peut être dû à la suppression de l'effet stimulant de la lumière sur cette enzyme en début de journée. Aucune trace du 15N03- absorbé ne se décèle dans les bactéroïdes. Il s'en accumule dans la fraction végétale des nodosités. L'augmentation de la teneur en nitrite, légèrement supérieure dans les nodosités à R. Japonicum NR+ par rapport aux nodosités à R. Japonicum NR- témoigne cependant de la réduction du nitrate dans ces organes.
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Thieme, Sebastian. "Entwurf und Verwendung eines Microarrays zur Untersuchung des Genistein-Stimulons bei Bradyrhizobium japonicum." Doctoral thesis, Technische Universität Dresden, 2006. https://tud.qucosa.de/id/qucosa%3A23971.

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Das Bakterium Bradyrhizobium japonicum ist wie andere Rhizobien in der Lage mit Pflanzen der Familie Fabales (Leguminosen) eine Symbiose einzugehen. Im symbiontischen Zustand fixieren die Mikrosymbionten atmosphärischen, molekularen Stickstoff und stellen diesen den Pflanzen in verwertbarer Form zur Verfügung. Im Gegenzug erhalten die Bakterien von den Pflanzen verschiedene Verbindungen als Kohlenstoff- und Energiequelle. Dem geht ein komplexer Signalaustausch voraus um die gegenseitige Erkennung der Partner und die Spezies-spezifische Symbiose zu ermöglichen. Auf Seite der Bakterien erfolgt die Reaktion auf die Gegenwart pflanzlicher Signalmoleküle. In B. japonicum induziert das Flavonoid Genistein die Transkription einer Reihe von Genen. Durch die Expression der nod-Gene erfolgt eine Synthese von Lipochitooligosacchariden. Diese sogenannten Nodulationsfaktoren rufen wiederum eine Reaktion in der Wirtspflanze hervor. Um die zugrunde liegende Genexpression und deren Regulationsmechanismen zu erforschen, standen bis dato nur Methoden für die Untersuchung einzelner Gene zur Verfügung. Die erstmals 1995 publizierte Microarraytechnologie eröffnete die Möglichkeit zu einem Zeitpunkt die Gentranskription eines gesamten Organismus zu untersuchen (Schena et al. 1995). Um mit dieser Technologie die Gentranskription bei B. japonicum zu untersuchen, wurde ein auf PCR-Produkten basierender Microarray hergestellt. Grundlage für die Synthese genspezifischer PCR-Produkte war die symbiontische Region von B. japonicum und andere zu diesem Zeitpunkt bekannte Gensequenzen (Göttfert et al. 2001). Nach der 2002 erfolgten Veröffentlichung des Genoms von B. japonicum erfolgte ein Abgleich der bisher verwendeten Sequenzen mit der vollständigen Sequenzinformation (Kaneko et al. 2002a). Nach Synthese der PCR-Produkte und deren Kontrolle durch Sequenzierung erfolgte die Herstellung des Microarrays unter Einsatz eines Microarrayspotters. Geeignete Techniken der cDNA-Markierung und die Microarray-Hybridisierung wurden mit Total-RNA von B. japonicum-Kulturen ausgetestet und etabliert. Eine differentielle Expremierung war eine Stunde nach Genistein- bzw. Methanolgabe zum Kulturmedium nicht nachweisbar. Zum darauffolgenden Zeitpunkt konnte die bekannte Induktion der nod-Gene durch dass Flavonoid Genistein beobachtet werden. Diese Induktion fiel in den verbleibenden zwei Zeitpunkten stark ab. Dieser Abfall der Induktionsrate ließ sich nicht mit dem Genisteingehalt im Kulturmedium erklären, da dieser Zeitraum konstant blieb. Vier Stunden nach Genisteingabe war die Induktion der Gene nolA und nodD2, deren Produkte sind an der negativen Regulation der nod-Gene beteiligt, nachweisbar. Dies wäre eine mögliche Erklärung für den Abfall der Induktion der nod-Gene. Im gleichen untersuchten Zeitraum wurde die Transkription verschiedener Gene der Stickstofffixierung und Denitrifikation durch das Flavonoid Genistein induziert. Auch für die bekannten Regulatoren dieser Gene war eine Induktion nachweisbar. Nach bisherigen Arbeiten war die Expression dieser Gene auf mikroaerobe und anaerobe Zustände, wie z.B. dem symbiontischen Stadium, beschränkt. Eine Induktion durch Flavonoide ist bisher nicht beobachtet worden. Um die Verwendbarkeit des Microarrays auch für den symbiontischen Zustand von B. japonicum zu testen, wurden Versuche mit Wurzelknöllchen von Sojabohne (Glycine max) durchgeführt. Dafür wurden Keimlinge der Sojabohne (G. max) mit B. japonicum inokuliert und die Total-RNA der entstehenden Wurzelknöllchen isoliert. Jedoch war eine Kreuzhybridisierung mit pflanzlicher Total-RNA zu beobachten. Der auf PCR-Produkten basierende Microarray ist für die Untersuchung des symbiontischen Stadiums von B. japonicum nicht einsetzbar. Im Vergleich dazu wurde ein auf Oligomeren basierender, kommerzieller Microarray für diesen Versuch getestet. Die Kreuzhybridisierung mit pflanzlicher Total-RNA war auf die Oligomere für die 16S rDNA und 23S rDNA reduziert.
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Books on the topic "Rhizobium japonicum"

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Payne, John Howard. Interaction of Rhizobium japonicum with soybean isolines carrying unique genes which affect nodulation at the Rj1 locus. 1985.

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Book chapters on the topic "Rhizobium japonicum"

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Appelbaum, E., N. Chartrain, D. Thompson, K. Johansen, M. O’Connell, and T. Mcloughlin. "Genes Of Rhizobium Japonicum Involved in Development of Nodules." In Nitrogen fixation research progress, 101–7. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5175-4_14.

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Fernández, V. M., R. Munilla, M. Barate, and P. Reyes. "Redox Properties of the Hydrogenase of Chemolithotrophically Grown Rhizobium japonicum." In Inorganic Nitrogen Metabolism, 175–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71890-8_31.

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Lambert, G. R., A. R. Harker, M. Zuber, D. A. Dalton, F. J. Hanus, S. A. Russell, and H. J. Evans. "Characterization, Significance and Transfer of Hydrogen Uptake Genes from Rhizobium Japonicum." In Nitrogen fixation research progress, 209–15. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5175-4_28.

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Ronson, C. W., A. Bosworth, M. Genova, S. Gudbrandsen, T. Hankinson, R. Kwiatkowski, H. Ratcliffe, et al. "Field release of genetically-engineered Rhizobium meliloti and Bradyrhizobium japonicum strains." In Nitrogen Fixation, 397–403. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-6432-0_41.

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O’Gara, F., B. Boesten, M. O’Regan, B. Kiely, B. Higgisson, C. Condon, K. Birkenhead, and S. Manian. "Molecular Biology of Genes Involved in Carbon Metabolism in Rhizobium Meliloti and Bradyrhizobium Japonicum." In Molecular genetics of plant-microbe interactions, 295–97. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_74.

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Atherly, Alan G., R. K. Prakash, Robert V. Masterson, Nancy B. DuTeau, and Kim S. Engwall. "The Organization of Genes Involved in Symbiotic Nitrogen Fixation on Indigenous Plasmids of Rhizobium Japonicum." In World Soybean Research Conference III: Proceedings, 291–300. New York: CRC Press, 2022. http://dx.doi.org/10.1201/9780429267932-50.

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Masloboeva, Nadezda, Hauke Hennecke, and Hans-Martin Fischer. "Rhizobial Extracytoplasmic Function (ECF) σ Factors and Their Role in Oxidative Stress Response ofBradyrhizobium japonicum." In Biological Nitrogen Fixation, 307–14. Hoboken, NJ, USA: John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119053095.ch30.

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VERSSIANI, J. B. S., M. A. S. VERSSIANI, I. B. BORGES, A. M. AMARAL, and T. S. CAIXETA. "Doses de fósforo e inoculação com Bradyrhizobium nas características agronômicas do feijão-caupi." In Produção Animal e Vegetal: Inovações e Atualidades. Agron Food Academy, 2021. http://dx.doi.org/10.53934/9786599539633-3.

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O feijão-caupi é considerado uma das leguminosas mais cultivadas no mundo, sendo importante fonte de proteína para a alimentação humana. Uma prática crescente nos últimos anos é o uso de inoculantes com estirpes de bactérias do gênero Rhizobium, as quais intervêm na fixação do nitrogênio atmosférico ao solo pelas leguminosas. Objetivou-se com este trabalho avaliar a interação entre diferentes doses de fósforo adicionadas ao solo e ausência e presença de inoculação com bactérias do gênero Bradyrhizobium na cultura do feijão-caupi, em Arinos-MG. O experimento foi conduzido em vasos com capacidade de 12 L. O delineamento experimental utilizado foi em blocos ao acaso no esquema fatorial 4 x 2, com seis repetições, sendo os tratamentos do Fator A constituídos pelas seguintes doses: 0, 45, 90 e 180 kg ha-1 de P2O5, aplicados ao solo, e do Fator B: ausência e presença de inoculação com Bradyrhizobium japonicum estirpe 3262. Foram avaliadas as características alométricas e produtivas: vagens por planta, número de grãos por vagem, o comprimento de vagem, massa de cem grãos, massa seca da raiz e a produtividade de grãos. Não houve interação entre os fatores estudados, apenas efeito isolado nas variáveis analisadas. As doses de P influenciaram todas as variáveis em estudo, enquanto que, os tratamentos sem ou com inoculação influenciaram apenas o número de vagem por planta e a produtividade. A dose de fósforo com máxima eficiência econômica foi de 164,64 kg ha-1 de P2O5, resultando em produtividade média de 3650 kg ha-1 de grãos de feijão-caupi.
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Conference papers on the topic "Rhizobium japonicum"

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Rohmani, Risma Wakhidatur, Iqbal Erdiansyah, and FNU Djenal. "Karakteristik Bakteri Rhizobium japonicum Bintil Akar Kedelai pada Cekaman Salinitas Bertingkat." In Seminar Nasional Semanis Tani Polije 2020. Politeknik Negeri Jember, 2020. http://dx.doi.org/10.25047/agropross.2020.41.

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Kenaikan suhu permukaan bumi berimbas pada naiknya permukaan air laut ke daratan, hal inilah yang menyebabkan garam-garam yang dikandung oleh air laut akan mengalami sedimentasi di tanah yang dilewatinya. Tanaman kedelai yang tercekam salin dapat menurunkan hasil produksi tanaman karena garam yang terlarut dapat menurunkanpotensial larutan tanah sehingga tanaman mengalami kekurangan air. Teknologi khusus yang digunakan untuk menunjang pertubuhan dan hasil tanaman kedelai di lahan salin adalah penggunaan pupuk hayati, yang salah satunya berasal dari Rhizobium japonicum.Rhizobium japonicum merupakan bakteri rizosfer yang mampu menguraikan nitrogen bebas di udara menjadi unsur yang mampu terserap oleh tanaman. Nitrogen adalah unsur hara yang sangat penting dalam proses fotosintesis tanaman untuk menghasilkan fotosintat yang berguna untuk pertumbuhan dan produksi yang optimal. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri Rhizobium japonicum pada tingkat salinitas tertentu pada media YEMA+Congo Red,penelitian ini dilakukan pada bulan Juni 2019 sampai dengan September 2019 di Laboratorium Biosain Politeknik Negeri Jember. Penelitian ini dilakukan dengan menggunakan rancangan non parametrik dengan berbagai macam tingkat cekaman salinitas pada media yaitu: 0 ppm, 1000 ppm, 2500 ppm, 4000 ppm, dan 8000 ppm. Hasil penelitian menunjukkan koloni bakteri Rhizobium japonicum berbentuk bulat cembung berwarna putih sampai merah muda dan ukuran sel 2µ-4µ.
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Ananyeva, I. N., Z. M. Aleschenkova, P. V. Rybaltovskaya, and M. A. Chindareva. "Effect of soybean (Glycine max (L.) Merill) treatments on the introduction capacity of endophytic bacteria." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-103.

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The goal of the work was to obtain antibiotic-resistant forms of endophytic Glycine max L. (Merill) bacteria and to study their introduction potential affected by different seed treatment methods. Rifampicin-resistant variants of endophytic soybean bacteria Rhizobium radiobacter 27c and Pseudomonas fluorescens 11E preserving valuable properties were derived. Soybean seed treatment with Bradyrhizobium japonicum BIM V-501D and endophytic nitrogen-fixing Rh. radiobacter 27c, phosphate-mobilizing Ps. fluorescens 11E bacteria under model conditions promoted accumulation of nitrogen-fixing bacteria in the root, stem and leaves. The number of nodules rose by 70% compared with the mono-inoculated control; plant height increased by 19%.
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Didovich, S. V., O. P. Alekseenko, and A. N. Pas'. "Symbiotic efficiency of nodule bacteria strains on legumes." In РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.08.

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Nowadays, ecologization is one of the important tasks for agriculture. Microbiological preparations based on nodule bacteria application in agriculture is a significant part of organic crop production. Symbiotic efficiency of 13 nodule bacteria strains from the Crimean collection of microorganisms of the FSBSI “Research Institute of Agriculture of Crimea” (http://www.ckp- rf.ru/usu/507484/) was studied in our research. In laboratory conditions, we established that four strains of Rhizobium leguminosarum bv. viceae and five strains of Bradуrhizobium japonicum have high symbiotic efficiency to Pisum sativum L., Lathyrus sativus L., Glycinе max L.(Merr.). These strains are recommended for identifying highly effective ones to modern cultivars of these legumes in the conditions of the steppe zone of Crimea.
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Ghiron, Camillo A., Maurice R. Eftink, James K. Waters, and David W. Emerich. "Fluorescence studies with malate dehydrogenase from rhizobium japonicum 3I1B-143 bacteroids: a two-tryptophan containing protein." In OE/LASE '90, 14-19 Jan., Los Angeles, CA, edited by Joseph R. Lakowicz. SPIE, 1990. http://dx.doi.org/10.1117/12.17725.

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Yakimenko, M. V., S. A. Begun, and A. I. Sorokina. "Species diversity of natural rhizobia populations of the Russian Far East." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.279.

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The specificity of the soils of the Far East is the presence of aboriginal soybean nodule bacteria in them. A detailed study of the morphological and cultural, physiological and economically useful properties of these microorganisms made it possible to identify the most valuable strains of B. japonicum, S. fredii, B. elkanii from the Far Eastern natural populations for their preservation in the collection.
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