Academic literature on the topic 'Rhizobium leguminosarum Molecular aspects'

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Journal articles on the topic "Rhizobium leguminosarum Molecular aspects"

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Chalifour, François-P., and Nicole Benhamou. "Indirect evidence for cellulase production by Rhizobium in pea root nodules during bacteroid differentiation: cytochemical aspects of cellulose breakdown in rhizobial droplets." Canadian Journal of Microbiology 35, no. 9 (September 1, 1989): 821–29. http://dx.doi.org/10.1139/m89-138.

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Cytochemical localization of cellulosic β-(1–4) glucans in pea (Pisum sativum L.) nodules at different stages of infection by an effective isolate of Rhizobium leguminosarum biovar viceae was studied using a gold-complexed exoglucanase. Cellulose subunits were present in great amounts in root cell walls, as shown by intense and regular labeling by gold particles. Labeling was unevenly distributed over the thin walls of emerging infection threads. In more developed infection threads, labeling was more intense and evenly distributed than in emerging threads, although slightly altered, unlabeled
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Russo, Daniela M., Alan Williams, Anne Edwards, Diana M. Posadas, Christine Finnie, Marcelo Dankert, J. Allan Downie, and Angeles Zorreguieta. "Proteins Exported via the PrsD-PrsE Type I Secretion System and the Acidic Exopolysaccharide Are Involved in Biofilm Formation by Rhizobium leguminosarum." Journal of Bacteriology 188, no. 12 (June 15, 2006): 4474–86. http://dx.doi.org/10.1128/jb.00246-06.

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ABSTRACT The type I protein secretion system of Rhizobium leguminosarum bv. viciae encoded by the prsD and prsE genes is responsible for secretion of the exopolysaccharide (EPS)-glycanases PlyA and PlyB. The formation of a ring of biofilm on the surface of the glass in shaken cultures by both the prsD and prsE secretion mutants was greatly affected. Confocal laser scanning microscopy analysis of green-fluorescent-protein-labeled bacteria showed that during growth in minimal medium, R. leguminosarum wild type developed microcolonies, which progress to a characteristic three-dimensional biofilm
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Figueira, Etelvina Maria de Almeida Paula, Ana Isabel Gusmão Lima, and Sofia Isabel Almeida Pereira. "Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae: glutathione as a detoxifying agent." Canadian Journal of Microbiology 51, no. 1 (January 1, 2005): 7–14. http://dx.doi.org/10.1139/w04-101.

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Rhizobium leguminosarum bv. viciae strains expressing different degrees of tolerance to metal stress were used in this work to study the basic mechanisms underlying heavy metal tolerance. We used various parameters to evaluate this response. The strains' growth responses under different Cd2+ concentrations were determined and we reported variation in Cd2+ tolerance. Total soluble protein content decreased drastically, revealing the toxic effects that intracellular Cd2+ imposes on cellular metabolism, but this decrease in protein content was particularly evident in sensitive and moderately tole
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Shahzad, Farood, Muhammad Kamran Taj, Ferhat Abbas, Muhammad Shafee, Safed Ahmed Essote, Imran Taj, and Abdul Manan Achakzai. "Microbiological studies on Rhizobium leguminosarum isolated from pea (Pisum sativum L.)." Bangladesh Journal of Botany 48, no. 4 (December 31, 2019): 1223–29. http://dx.doi.org/10.3329/bjb.v48i4.49079.

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Rhizobia are the true bacteria that establish symbiotic relationship leading to the development of new root nodules. This study has been designed to evaluate the microbiological aspects of Rhizobium leguminosarum in target area. A total of 1000 (200 from each site) roots were collected from five different agriculture fields (Quetta, Pishin, Killa Abdulla, Kuchlak and Hanna Urak) and screened through different standard microbiological procedures. Results revealed that 665/1000 (66.5%) roots samples were positive for Rhizobium leguminosarum. The highest percentage was from Pishin 180/200 (18%) a
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Enibukun, Jesupemi Mercy, and Bolatito Esther Boboye. "Molecular characterization and evaluation of crude oil remediation potential of some rhizobia isolated from plant root nodules." Nova Biotechnologica et chimica 19, no. 1 (June 30, 2020): 80–88. http://dx.doi.org/10.36547/nbc.v19i1.580.

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This study aimed to determine the molecular identities and genetic relatedness of rhizobia isolated from pigeon pea and pinto beans, and assess their remediation potential in the presence of 1 %, 3 % and 5 % (w/v) crude oil in minimal medium for 7 days incubation period. Standard microbiological and molecular methods which include amplification and purification of 16S rRNA, agarose gel electrophoresis, and sequencing. Results showed molecular identities of six rhizobia from pigeon peas as Bradyrhizobium diazoefficiens USDA122, Rhizobium leguminosarum WSM2304, Bradyrhizobium japonicum N61, Rhiz
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Soberón-Chávez, Gloria, and Rebeca Nájera. "Isolation from soil of Rhizobium leguminosarum lacking symbiotic information." Canadian Journal of Microbiology 35, no. 4 (April 1, 1989): 464–68. http://dx.doi.org/10.1139/m89-071.

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Bacteria resembling Rhizobium leguminosarum, but lacking symbiotic information, were isolated from soil of two different geographical origins. One of these bacteria belongs to a previously described Rhizobium leguminosarum bv. phaseoli somatic serogroup, is fully complemented for nodulation and nitrogen fixation by an R. leguminosarum bv. phaseoli symbiotic plasmid, and is able to compete for bean nodulation with indigenous R. leguminosarum bv. phaseoli strains. This is the first report giving evidence for persistence in soil of Rhizobium lacking symbiotic information.Key words: Rhizobium ecol
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Laguerre, Gisèle, Marc Bardin, and Noëlle Amarger. "Isolation from soil of symbiotic and nonsymbiotic Rhizobium leguminosarum by DNA hybridization." Canadian Journal of Microbiology 39, no. 12 (December 1, 1993): 1142–49. http://dx.doi.org/10.1139/m93-172.

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A procedure based upon DNA hybridization was developed for the specific detection of Rhizobium leguminosarum and its different biovars among bacteria isolated from soil. DNA colony hybridization and restriction fragment length polymorphism analysis with a R. leguminosarum chromosomal probe were found to be species specific for R. leguminosarum and Rhizobium etli. By using R. leguminosarum nod gene probes, biovar specificity was obtained. Of 302 soil isolates screened for their inability to grow on Luria-Bertani agar medium, 13 strains could be assigned to the R. leguminosarum species on the ba
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Rioux, Clément R., D. Carlyle Jordan, and James B. M. Rattray. "Anthranilate-promoted iron uptake in Rhizobium leguminosarum." Archives of Biochemistry and Biophysics 248, no. 1 (July 1986): 183–89. http://dx.doi.org/10.1016/0003-9861(86)90415-7.

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Velázquez, Encarna, Esperanza Martı́nez-Romero, Dulce Nombre Rodrı́guez-Navarro, Martha E. Trujillo, Antonio Daza, Pedro F. Mateos, Eustoquio Martı́nez-Molina, and Peter van Berkum. "Characterization of Rhizobial Isolates of Phaseolus vulgaris by Staircase Electrophoresis of Low-Molecular-Weight RNA." Applied and Environmental Microbiology 67, no. 2 (February 1, 2001): 1008–10. http://dx.doi.org/10.1128/aem.67.2.1008-1010.2001.

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ABSTRACT Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli,Rhizobium gallicum, Rhizobium giardinii,Rhizobium leguminosarum bv. viciae and bv. trifolii, andSinorhizobium fredii.
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Kucey, R. M. N., and M. F. Hynes. "Populations of Rhizobium leguminosarum biovars phaseoli and viceae in fields after bean or pea in rotation with nonlegumes." Canadian Journal of Microbiology 35, no. 6 (June 1, 1989): 661–67. http://dx.doi.org/10.1139/m89-107.

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Populations of Rhizobium leguminosarum bv. phaesoli and bv. viceae in southern Alberta soils were measured over a period of 4 years using a most probable number method. Five fields cropped to bean (Phaseolus vulgaris L.), five fields cropped to pea (Pisum sativum L.), and two fields cropped to wheat were used as test sites. Legume crops had received appropriate legume inoculants. Fields were sampled in the fall of the crop year and in the spring of the following 3 years during which fields were cropped to nonlegumes or left fallow. Numbers of R. leguminosarum bv. phaseoli were 100 to 1000 time
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Dissertations / Theses on the topic "Rhizobium leguminosarum Molecular aspects"

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Heinrich, Keith. "Ecological and molecular studies on rhizobial rhizopines." Title page, contents and summary only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phh469.pdf.

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Corrigendum attached to back cover. Includes bibliographical references (leaves 160-190). Investigates the role of rhizopines in rhizobial competition for nodulation, and to isolate the rhizopine synthesis genes in Rhizobium leguminosarum bv. viciae.
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Bahar, Masoud. "Molecular biology of rhizopine genes in Rhizobium leguminosarum br. viciae /." Title page, table of contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phb1508.pdf.

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Simpkins, Sean A. "The DnaK molecular chaperone of Rhizobium leguminosarum." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302035.

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Stevens, James B. "The molecular genetics of iron uptake in rhizobium leguminosarum." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323075.

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Rossen, L. "Molecular analysis of the nodulation genes of Rhizobium leguminosarum." Thesis, University of East Anglia, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370396.

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Sindhu, Satyavir Singh. "Molecular analysis of lipopolysaccharide and membrane associated proteins in Rhizobium leguminosarum." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.256988.

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This study describes the use of monoclonal antibodies to investigate molecular components of the Rhizobium cell surface that might be important for symbiotic interactions with the host legume. Components that have been identified include lipopolysaccharide and both membrane-associated and secreted proteins. Differences were observed in the structure and antigenicity of lipopolysaccharide (LPS) from free-living rhizobia compared with that of endosymbiotic bacteroids. Culture pH, oxygen concentration and carbon source were all found to be important factors that could affect the expression of LPS
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Stinnett, Joshua. "The Chelation of Metal Ions by Vicibactin, a Siderophore Produced by Rhizobium leguminosarum ATCC 14479." Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/honors/485.

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Vicibactin is a small, high-affinity iron chelator produced by Rhizobium leguminosarum ATCC 14479. Previous work has shown that vicibactin is produced and secreted from the cell to sequester ferric iron from the environment during iron-deplete conditions. This ferric iron is then transported into the cell to be converted into ferrous iron. This study uses UV-Vis spectroscopy as well as ion trap-time of flight mass spectroscopy to determine that vicibactin does form a complex with copper(II) ions, however, at a much lower affinity than for iron(III). Stability tests have shown that the copper(I
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Cañete, Morales Alejandro Ignacio. "Caracterización molecular de aislados silvestres chilenos de Rhizobium a través del uso de marcadores moleculares basados en amplificación por RFLP-PCR." Tesis, Universidad de Chile, 2007. http://www.repositorio.uchile.cl/handle/2250/101862.

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Barisic, Valeria. "Characterization of Putative ExbB and ExbD Leads to the Identification of a Potential Tol-Pal System in Rhizobium leguminosarum ATCC 14479." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etd/2489.

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Rhizobium leguminosarum is a Gram negative nitrogen-fixing soil bacterium. Due to the limited bioavailability of iron, bacteria utilize siderophores that scavenge and bind available iron. The transport of iron-siderophore complexes is achieved by the TonB-ExbB-ExbD complex. We have previously shown that a functional TonB protein is necessary for iron transport by creating ΔtonB mutants and assessing their growth and 55Fe-siderophore transport ability. We attempted to identify and characterize the roles of putative exbB and exbD genes using a similar approach. Growth curves and sequence analyse
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Thaha, Fathuma Zuleikha. "Characterization of acetate metabolism genes in Sinorhizobium, Rhizobium, meliloti." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0016/MQ55093.pdf.

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Books on the topic "Rhizobium leguminosarum Molecular aspects"

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Rossen, Lone. Molecular analysis of the nodulation genes of "Rhizobium leguminosarum". Norwich: University of East Anglia, 1985.

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2

Sindhu, Satyavir Singh. Molecular analysis of lipopolysaccharide and membrane associated proteins in Rhizobium Leguminosarum. Norwich: University of East Anglia, 1990.

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Book chapters on the topic "Rhizobium leguminosarum Molecular aspects"

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Downie, J. A., B. P. Surin, I. J. Evans, L. Rossen, J. L. Firmin, C. A. Shearman, and A. W. B. Johnston. "Nodulation Genes of Rhizobium Leguminosarum." In Molecular genetics of plant-microbe interactions, 225–28. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_56.

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Colonna-Romano, S., R. Defez, M. Filser, M. Guida, M. Iaccarino, A. Lamberti, A. Riccio, et al. "Glutamine Synthetases of Rhizobium Leguminosarum." In Molecular genetics of plant-microbe interactions, 255–57. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_64.

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Hong, G. F., J. L. Burn, and A. W. B. Johnston. "Analysis of the Mechanism of nod Gene Regulation in Rhizobium leguminosarum." In Plant Molecular Biology, 523–30. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-7598-6_48.

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Ruiz-Argüeso, T., E. Hidalgo, J. Murillo, L. Rey, and J. M. Palacios. "Molecular Genetics of the Hydrogen Uptake System of Rhizobium Leguminosarum." In Advances in Molecular Genetics of Plant-Microbe Interactions Vol. 1, 222–25. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-015-7934-6_34.

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Manian, S. S., P. Grönger, U. B. Priefer, and A. Pühler. "Identification, Characterisation and Sequence Analysis of the Rhizobium Leguminosarum NifA Gene." In Molecular genetics of plant-microbe interactions, 276–78. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_69.

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Vijn, Irma, Ton van Brussel, Albert van Kammen, and Ton Bisseling. "The vetch (Vicia) and Rhizobium leguminosarum bv. viciae symbiosis: A system to study the activity of Rhizobium Nod factors." In Plant Molecular Biology, 203–18. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78852-9_20.

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Sutton, J. M., R. Rivilla, A. E. Davies, E. J. A. Lea, S. Ghelani, C. Finnie, G. Dean, and J. A. Downie. "Functional Analysis of Nodo and Nodt from Rhizobium Leguminosarum Biovar Viciae." In Advances in Molecular Genetics of Plant-Microbe Interactions, 103–6. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0177-6_15.

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Tichy, H. V., C. Schild, H. M. Ripke, L. M. Nelson, H. Fees, and W. Lotz. "Analysis of hup DNA and Hup host range of Rhizobium leguminosarum BIO." In Molecular genetics of plant-microbe interactions, 279–81. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4482-4_70.

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Franssen, Henk J., Irma Vijn, Wei Cai Yang, and Ton Bisseling. "Developmental aspects of the Rhizobium-legume symbiosis." In 10 Years Plant Molecular Biology, 89–107. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2656-4_6.

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Spaink, Herman P., Guido V. Bloemberg, André H. M. Wijfjes, Tita Ritsema, Otto Geiger, Isabel M. López-Lara, Marga Harteveld, et al. "The Molecular Basis of Host Specificity in the Rhizobium Leguminosarum-Plant Interaction." In Advances in Molecular Genetics of Plant-Microbe Interactions, 91–98. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0177-6_13.

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Reports on the topic "Rhizobium leguminosarum Molecular aspects"

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Ron, Eliora, and Eugene Eugene Nester. Global functional genomics of plant cell transformation by agrobacterium. United States Department of Agriculture, March 2009. http://dx.doi.org/10.32747/2009.7695860.bard.

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The aim of this study was to carry out a global functional genomics analysis of plant cell transformation by Agrobacterium in order to define and characterize the physiology of Agrobacterium in the acidic environment of a wounded plant. We planed to study the proteome and transcriptome of Agrobacterium in response to a change in pH, from 7.2 to 5.5 and identify genes and circuits directly involved in this change. Bacteria-plant interactions involve a large number of global regulatory systems, which are essential for protection against new stressful conditions. The interaction of bacteria with
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