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Journal articles on the topic 'Ribonuclease B'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Arnold, Ulrich, and Renate Ulbrich-Hofmann. "Kinetic and Thermodynamic Thermal Stabilities of Ribonuclease A and Ribonuclease B†." Biochemistry 36, no. 8 (February 1997): 2166–72. http://dx.doi.org/10.1021/bi962723u.

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2

SHINOMIYA, TAKAHISA, NOZOMI NISHIMURA, KOICHI TAMOTO, and TSUTOMU HONJOH. "BINDING OF HUMAN PLACENTAL RIBONUCLEASE INHIBITOR TO EXTRACELLULAR MATRIX MOLECULES ." Biomedical Research 14, no. 2 (1993): 123–28. http://dx.doi.org/10.2220/biomedres.14.123.

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3

Liu, Jun. "Targeted ribonuclease can inhibit replication of hepatitis B virus." World Journal of Gastroenterology 9, no. 2 (2003): 295. http://dx.doi.org/10.3748/wjg.v9.i2.295.

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4

Zapun, André, Stefana M. Petrescu, Pauline M. Rudd, Raymond A. Dwek, David Y. Thomas, and John J. M. Bergeron. "Conformation-Independent Binding of Monoglucosylated Ribonuclease B to Calnexin." Cell 88, no. 1 (January 1997): 29–38. http://dx.doi.org/10.1016/s0092-8674(00)81855-3.

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5

Ko, T. P., R. Williams, and A. McPherson. "Structure of a ribonuclease B+d(pA)4 complex." Acta Crystallographica Section D Biological Crystallography 52, no. 1 (January 1, 1996): 160–64. http://dx.doi.org/10.1107/s0907444995009127.

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6

Williams, G. A., U. Macevilly, R. Ryan, and M. G. Harrington. "Stabilization of Ribonuclease B Activity by Concentrated Xylose Solutions." Biochemical and Biophysical Research Communications 207, no. 1 (February 1995): 432–37. http://dx.doi.org/10.1006/bbrc.1995.1206.

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7

Escola, J. M., J. C. Grivel, P. Chavrier, and J. P. Gorvel. "Different endocytic compartments are involved in the tight association of class II molecules with processed hen egg lysozyme and ribonuclease A in B cells." Journal of Cell Science 108, no. 6 (June 1, 1995): 2337–45. http://dx.doi.org/10.1242/jcs.108.6.2337.

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The processing of exogenous antigens and the association of peptides with class II molecules both occur within the endocytic pathway. 2A4 B lymphoma cells of the H-2k haplotype were grown in the presence or the absence of two different exogenous antigens (hen egg lysozyme and ribonuclease A) internalized by fluid-phase endocytosis. Using subcellular fractionation techniques, we demonstrate that, in the presence of hen egg lysozyme, newly synthesized SDS-stable class II molecules are detected in a dense endocytic compartment which does not have the characteristics of neither early and late endosomes nor lysosomes. In contrast, no SDS-stable class II molecules are observed between ribonuclease A and newly synthesized class II molecules. Interestingly, when class II molecules are analyzed at steady state, SDS-stable class II molecules induced by ribonuclease A are found in a compartment cosedimenting with late endosomes. These results suggest that the tight associations between ribonuclease A or hen egg lysozyme with class II molecules occur in distinct endocytic compartments and that these associations may depend on the sensitivity of antigens to proteolysis.

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8

Camilleri, P., N. J. Haskins, P. M. Rudd, and M. R. Saunders. "Applications of electrospray mass spectrometry to studies on the structural properties of ribonuclease A and ribonuclease B." Rapid Communications in Mass Spectrometry 7, no. 5 (May 1993): 332–35. http://dx.doi.org/10.1002/rcm.1290070505.

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9

Williams, R. L., S. M. Greene, and A. McPherson. "The crystal structure of ribonuclease B at 2.5-A resolution." Journal of Biological Chemistry 262, no. 33 (November 1987): 16020–31. http://dx.doi.org/10.1016/s0021-9258(18)47690-9.

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10

Tavis, John E., Grigoris Zoidis, Marvin J. Meyers, and Ryan P. Murelli. "Chemical Approaches to Inhibiting the Hepatitis B Virus Ribonuclease H." ACS Infectious Diseases 5, no. 5 (March 22, 2018): 655–58. http://dx.doi.org/10.1021/acsinfecdis.8b00045.

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11

Tavis, John E., and Elena Lomonosova. "The hepatitis B virus ribonuclease H as a drug target." Antiviral Research 118 (June 2015): 132–38. http://dx.doi.org/10.1016/j.antiviral.2015.04.002.

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12

Urbanikova, Lubica, and Jozef Sevcik. "Crystallization and preliminary X-ray investigation of the complex of RNase Sa with wild-type barstar." Acta Crystallographica Section D Biological Crystallography 54, no. 3 (May 1, 1998): 403–4. http://dx.doi.org/10.1107/s0907444997010688.

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RNase Sa, an extracellular ribonuclease produced by Streptomyces aureofaciens, is inhibited by barstar, the natural protein inhibitor of barnase, the ribonuclease of Bacillus amyloliquefaciens. The complex of RNase Sa with wild-type barstar was crystallized by hanging-drop vapour diffusion. It was shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis that RNase Sa and barstar are present in equimolar proportions in the crystals. The crystals are in the hexagonal space group P65 with unit cell dimensions a = b = 56.95, c = 135.8 Å. They diffract to 1.7 Å resolution at the DESY synchronton source. The asymmetric unit contains one molecule of the complex.

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13

PITCHAYAWASIN, Suthasinee, and Minoru ISOBE. "Mass Spectrometric Assignment of Smith Degradation Glycopeptides Derived from Ribonuclease B." Bioscience, Biotechnology, and Biochemistry 68, no. 7 (January 2004): 1424–33. http://dx.doi.org/10.1271/bbb.68.1424.

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14

Tavis, John E., Xiaohong Cheng, Yuan Hu, Michael Totten, Feng Cao, Eleftherios Michailidis, Rajeev Aurora, et al. "The Hepatitis B Virus Ribonuclease H Is Sensitive to Inhibitors of the Human Immunodeficiency Virus Ribonuclease H and Integrase Enzymes." PLoS Pathogens 9, no. 1 (January 22, 2013): e1003125. http://dx.doi.org/10.1371/journal.ppat.1003125.

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15

Bak, Ellen, Jennifer T. Miller, Andrea Noronha, John Tavis, Emilio Gallicchio, Ryan P. Murelli, and Stuart F. J. Le Grice. "3,7-Dihydroxytropolones Inhibit Initiation of Hepatitis B Virus Minus-Strand DNA Synthesis." Molecules 25, no. 19 (September 27, 2020): 4434. http://dx.doi.org/10.3390/molecules25194434.

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Initiation of protein-primed (-) strand DNA synthesis in hepatitis B virus (HBV) requires interaction of the viral reverse transcriptase with epsilon (ε), a cis-acting regulatory signal located at the 5’ terminus of pre-genomic RNA (pgRNA), and several host-encoded chaperone proteins. Binding of the viral polymerase (P protein) to ε is necessary for pgRNA encapsidation and synthesis of a short primer covalently attached to its terminal domain. Although we identified small molecules that recognize HBV ε RNA, these failed to inhibit protein-primed DNA synthesis. However, since initiation of HBV (-) strand DNA synthesis occurs within a complex of viral and host components (e.g., Hsp90, DDX3 and APOBEC3G), we considered an alternative therapeutic strategy of allosteric inhibition by disrupting the initiation complex or modifying its topology. To this end, we show here that 3,7-dihydroxytropolones (3,7-dHTs) can inhibit HBV protein-primed DNA synthesis. Since DNA polymerase activity of a ribonuclease (RNase H)-deficient HBV reverse transcriptase that otherwise retains DNA polymerase function is also abrogated, this eliminates direct involvement of RNase (ribonuclease) H activity of HBV reverse transcriptase and supports the notion that the HBV initiation complex might be therapeutically targeted. Modeling studies also provide a rationale for preferential activity of 3,7-dHTs over structurally related α-hydroxytropolones (α-HTs).

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16

Wieczorek, Eleonora, Janina Wiśniewska, and Bronisława Morawiecka. "Acid phosphatases and ribonucleases from Dactylis glomerata seeds. I. Chromatographic and electrophoretic heterogeneity of the enzymes." Acta Societatis Botanicorum Poloniae 47, no. 4 (2015): 441–53. http://dx.doi.org/10.5586/asbp.1978.040.

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Acid phosphatase and ribonuclease extracted with 0.1 M sodium acetate buffer, pH 5.1 from Dactylis glomerata seeds, and partially purified by means of 70% ethanol precipitation showed electrophoretic and Chromatographic heterogeneity. After chromatography on DEAE-cellulose acid phosphatase and ribonuclease were separated into four peaks. Nonadsorbing acid phosphatase on DEAE-cellulose (peak I) was separated into four peaks on CM-cellulose. The highest activity (11 units/mg) was found in fraction b (acid phosphatase Ib). The enzyme was activated by Mg<sup>2+</sup>, Ca<sup>2+</sup>, Li<sup>+</sup>, Cs<sup>+</sup>, K<sup>+</sup> ions and inhibited by Cu<sup>2+</sup>, Zu<sup>2+</sup>, F<sup>-</sup> and Mo<sup>-6</sup> at optimum pH 5.0. Strong absorbing ribonuclease on DEAE-cellulose (peak IV) was further separated on G-200 Sephadex into two molecular forms: RN-asa1 and RN-ase2. Ribonuclease l, a thermolabile enzyme with specific activity 807 units/mg, showed an optimal activity at pH 4.8-5.1.

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17

Michelsen, Nete V., Klaus Brusgaard, Qihua Tan, Mads Thomassen, Khalid Hussain, and Henrik T. Christesen. "Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray." ISRN Pathology 2011 (December 26, 2011): 1–12. http://dx.doi.org/10.5402/2011/275102.

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The use of formalin-fixed, paraffin-embedded (FFPE) tissue overcomes the most prominent issues related to research on relatively rare diseases: limited sample size, availability of control tissue, and time frame. The use of FFPE pancreatic tissue in GEM may be especially challenging due to its very high amounts of ribonucleases compared to other tissues/organs. In choosing pancreatic tissue, we therefore indirectly address the applicability of other FFPE tissues to gene expression microarray (GEM). GEM was performed on archived, routinely fixed, FFPE pancreatic tissue from patients with congenital hyperinsulinism (CHI), insulinoma, and deceased age-appropriate neonates, using whole-genome arrays. Although ribonuclease-rich, we obtained biologically relevant and disease-specific, significant genes; cancer-related genes; genes involved in (a) the regulation of insulin secretion and synthesis, (b) amino acid metabolism, and (c) calcium ion homeostasis. These results should encourage future research and GEM studies on FFPE tissue from the invaluable biobanks available at the departments of pathology worldwide.

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18

Prifti, Georgia-Myrto, Dimitrios Moianos, Erofili Giannakopoulou, Vasiliki Pardali, John Tavis, and Grigoris Zoidis. "Recent Advances in Hepatitis B Treatment." Pharmaceuticals 14, no. 5 (May 1, 2021): 417. http://dx.doi.org/10.3390/ph14050417.

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Hepatitis B virus infection affects over 250 million chronic carriers, causing more than 800,000 deaths annually, although a safe and effective vaccine is available. Currently used antiviral agents, pegylated interferon and nucleos(t)ide analogues, have major drawbacks and fail to completely eradicate the virus from infected cells. Thus, achieving a “functional cure” of the infection remains a real challenge. Recent findings concerning the viral replication cycle have led to development of novel therapeutic approaches including viral entry inhibitors, epigenetic control of cccDNA, immune modulators, RNA interference techniques, ribonuclease H inhibitors, and capsid assembly modulators. Promising preclinical results have been obtained, and the leading molecules under development have entered clinical evaluation. This review summarizes the key steps of the HBV life cycle, examines the currently approved anti-HBV drugs, and analyzes novel HBV treatment regimens.

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19

Xia, Chuan, Yuan-Chuan Chen, Hao Gong, Wenbo Zeng, Gia-Phong Vu, Phong Trang, Sangwei Lu, Jianguo Wu, and Fenyong Liu. "Inhibition of Hepatitis B Virus Gene Expression and Replication by Ribonuclease P." Molecular Therapy 21, no. 5 (May 2013): 995–1003. http://dx.doi.org/10.1038/mt.2013.37.

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20

Kanaya, S., and T. Uchida. "Comparison of the primary structures of ribonuclease U2 isoforms." Biochemical Journal 240, no. 1 (November 15, 1986): 163–70. http://dx.doi.org/10.1042/bj2400163.

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The primary structures of the two isoforms of ribonuclease U2, RNAases U2-A and U2-B, were analysed and compared with each other. Among the chymotryptic peptides obtained from the reduced and S-carboxymethylated enzymes, only peptides C-3 were different from each other in terms of chromatographic behaviour on reverse-phase h.p.l.c. On the basis of chemical analyses of these peptides, it was shown that RNAase U2-B had an isopeptide bond in which Asp-32 was linked to Gly-33 through the beta-carboxy group in its side chain instead of the alpha-carboxy group. Deamidation of Asn-32 in RNAase U2-A led to the formation of this unusual linkage. The previously reported sequence of RNAase U2 [Sato & Uchida (1975) Biochem. J. 145, 353-360] was corrected by changing amino acid residues at eight different positions and by inserting an asparagine residue at position 32. The numbering of the positions of amino acid residues located downstream of Asn-32 was therefore shifted by 1. Accordingly, RNAase U2-A was shown to be composed of 114 amino acid residues.

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21

Dementiev, A. A., G. P. Moiseyev, and S. V. Shlyapnikov. "Primary structure and catalytic properties of extracellular ribonuclease of bacillus circulans." FEBS Letters 334, no. 2 (November 15, 1993): 247–49. http://dx.doi.org/10.1016/0014-5793(93)81721-b.

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22

Lu, Gaofeng, Elena Lomonosova, Xiaohong Cheng, Eileen A. Moran, Marvin J. Meyers, Stuart F. J. Le Grice, Craig J. Thomas, et al. "Hydroxylated Tropolones Inhibit Hepatitis B Virus Replication by Blocking Viral Ribonuclease H Activity." Antimicrobial Agents and Chemotherapy 59, no. 2 (December 1, 2014): 1070–79. http://dx.doi.org/10.1128/aac.04617-14.

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ABSTRACTHepatitis B virus (HBV) remains a major human pathogen despite the development of both antiviral drugs and a vaccine, in part because the current therapies do not suppress HBV replication far enough to eradicate the virus. Here, we screened 51 troponoid compounds for their ability to suppress HBV RNaseH activity and HBV replication based on the activities of α-hydroxytropolones against HIV RNaseH, with the goal of determining whether the tropolone pharmacophore may be a promising scaffold for anti-HBV drug development. Thirteen compounds inhibited HBV RNaseH, with the best 50% inhibitory concentration (IC50) being 2.3 μM. Similar inhibition patterns were observed against HBV genotype D and C RNaseHs, implying limited genotype specificity. Six of 10 compounds tested against HBV replication in culture suppressed replication via blocking of viral RNaseH activity, with the best 50% effective concentration (EC50) being 0.34 μM. Eighteen compounds inhibited recombinant human RNaseH1, and moderate cytotoxicity was observed for all compounds (50% cytotoxic concentration [CC50] = 25 to 79 μM). Therapeutic indexes ranged from 3.8 to 94. Efficient inhibition required an intact α-hydroxytropolone moiety plus one or more short appendages on the tropolone ring, but a wide variety of constituents were permissible. These data indicate that troponoids and specifically α-hydroxytropolones are promising lead candidates for development as anti-HBV drugs, providing that toxicity can be minimized. Potential anti-RNaseH drugs are envisioned to be employed in combination with the existing nucleos(t)ide analogs to suppress HBV replication far enough to block genomic maintenance, with the goal of eradicating infection.

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23

Rudd, Pauline M., Ian G. Scragg, Eva Coghill, and Raymond A. Dwek. "Separation and analysis of the glycoform populations of ribonuclease B using capillary electrophoresis." Glycoconjugate Journal 9, no. 2 (April 1992): 86–91. http://dx.doi.org/10.1007/bf00731704.

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24

Huang, Wei, Qiang Yang, Midori Umekawa, Kenji Yamamoto, and Lai-Xi Wang. "Arthrobacter Endo-β-N-Acetylglucosaminidase Shows Transglycosylation Activity on Complex-Type N-Glycan Oxazolines: One-Pot Conversion of Ribonuclease B to Sialylated Ribonuclease C." ChemBioChem 11, no. 10 (May 18, 2010): 1350–55. http://dx.doi.org/10.1002/cbic.201000242.

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25

Walton, Cherie M., Catherine H. Wu, and George Y. Wu. "A Ribonuclease H−Oligo DNA Conjugate That Specifically Cleaves Hepatitis B Viral Messenger RNA." Bioconjugate Chemistry 12, no. 5 (September 2001): 770–75. http://dx.doi.org/10.1021/bc010018e.

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26

Tsuji, H., K. Murai, K. Akagi, and M. Fujishima. "Effects of recombinant leukocyte interferon on ribonuclease activities in serum in chronic hepatitis B." Clinical Chemistry 36, no. 6 (June 1, 1990): 913–16. http://dx.doi.org/10.1093/clinchem/36.6.913.

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Abstract Alkaline ribonuclease (RNase; EC 3.1.27.5) activities and 2',5'-oligoadenylate synthetase (2-5 AS; no EC no. assigned) activities in serum were measured in nine patients with hepatitis B e antigen-positive chronic hepatitis B before, during, and after treatment with recombinant human interferon alpha-2b for four weeks at daily doses ranging from 3 to 10 mega-units. Alkaline RNase activities in serum significantly increased from 65.8 +/- 9.5 units/L (mean +/- SD) to 84.3 +/- 11.9 units/L after the first week of therapy (P less than 0.001). (One unit of RNase activity is defined as that increasing the absorbance at 260 nm by 1.0 in 1 min). This increase persisted during and until two weeks after the end of the therapy, at which time the mean RNase activity in serum was still significantly increased (70.8 +/- 9.4 units/L, P less than 0.01). Before therapy, phosphocellulose chromatography of RNase showed five active peaks of enzyme activity, which were similar to that observed even when RNase activity increased immediately after therapy. There was a close correlation between RNase activities and the logarithm of 2-5 AS activities, measured simultaneously in each patient. We conclude that recombinant alpha-interferon therapy increases RNase activities in serum, associated with the increased 2-5 AS activities.

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27

Collin, Mattias, and Vincent A. Fischetti. "A Novel Secreted Endoglycosidase fromEnterococcus faecaliswith Activity on Human Immunoglobulin G and Ribonuclease B." Journal of Biological Chemistry 279, no. 21 (March 17, 2004): 22558–70. http://dx.doi.org/10.1074/jbc.m402156200.

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28

Prien, Justin M., David J. Ashline, Anthony J. Lapadula, Hailong Zhang, and Vernon N. Reinhold. "The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS." Journal of the American Society for Mass Spectrometry 20, no. 4 (April 2009): 539–56. http://dx.doi.org/10.1016/j.jasms.2008.11.012.

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29

Tramontano, Enzo, Angela Corona, and Luis Menéndez-Arias. "Ribonuclease H, an unexploited target for antiviral intervention against HIV and hepatitis B virus." Antiviral Research 171 (November 2019): 104613. http://dx.doi.org/10.1016/j.antiviral.2019.104613.

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30

Hu, Yuan, Xiaohong Cheng, Feng Cao, Ailong Huang, and John E. Tavis. "β-Thujaplicinol inhibits hepatitis B virus replication by blocking the viral ribonuclease H activity." Antiviral Research 99, no. 3 (September 2013): 221–29. http://dx.doi.org/10.1016/j.antiviral.2013.06.007.

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31

Kanaya, Shigenori, Motohisa Oobatake, Haruki Nakamura, and Morio Ikehara. "pH-Dependent thermostabilization of Escherichia coli ribonuclease HI by histidine to alanine substitutions." Journal of Biotechnology 28, no. 1 (March 1993): 117–36. http://dx.doi.org/10.1016/0168-1656(93)90129-b.

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32

Gugliotti, Lina A., Kiran B. Sakhuja, Hongsheng Wang, Julia Pinkhasov, Paul E. Love, Susana M. Cerritelli, Herbert Morse, and Robert J. Crouch. "Constitutive Lymphoid Expression of the Nuclear Form of RNase H1 Is Associated with a Developmental Bottleneck at the Pro-B Cell Stage of B Cell Differentiation." Blood 114, no. 22 (November 20, 2009): 4702. http://dx.doi.org/10.1182/blood.v114.22.4702.4702.

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Abstract Abstract 4702 The development of B lymphocytes and the process of lineage determination are initiated by expression of a set of transcriptional regulators leading to V(D)J recombination events initiated by double-strand DNA breaks. Subsequently, these recombinations form DNAs that permit transcription of immunoglobulin genes and translation of the corresponding mRNAs, first by joining the V(D)J DNA sequences, then by recombination, that generates various isotypes of immunoglobulins by class-switch recombination (CSR). Formation of R-loops, regions containing RNA/DNA hybrid and a displaced single-stranded DNA, have been shown to lead to recombination in bacteria, yeast, HeLa and chick cells. Expression in each of these cases of excess ribonuclease H1 (RNase H1), a class of enzymes that degrade RNA in RNA/DNA hybrids, has ameliorated the deleterious effects and decreased recombinational events associated with R-loop formation. R-loops have been observed following transcription of the switch regions that occurs during CSR. The possibility that R-loops are important in V(D)J recombination has not been addressed, and whether ribonucleases H (RNases H) play a role in this process is still uncertain. Transgenic (TG) mice that overexpress RNase H1 in B and T cells (M27F7) were employed in this study. FACS analysis of hematopoietic cells from TG mice revealed a decrease in pre-B cells in the bone marrow. The data indicate a block at the pro-B to pre-B stage of B cell development, which may be the result of apoptosis due to the failure to generate a productive VH-D-JH rearrangement and expression of the pre-B cell receptor. A few B cells that successfully passed these checkpoints predominately differentiated into marginal zone and B1a cells in the peripheral lymphoid organs of the TG mice. These data suggest that R-loops are important in H chain gene rearrangement. This research is supported by the Intramural Research Program of the National Institutes of Health, the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the National Institute of Allergy and Infectious Diseases. Disclosures: No relevant conflicts of interest to declare.

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33

CHATTERJEE, C., P. SINHA, and S. C. AGARWALA. "INTERACTIVE EFFECT OF BORON AND PHOSPHORUS ON GROWTH AND METABOLISM OF MAIZE GROWN IN REFINED SAND." Canadian Journal of Plant Science 70, no. 2 (April 1, 1990): 455–60. http://dx.doi.org/10.4141/cjps90-053.

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Boron-phosphorus interaction was observed in maize (Zea mays L. ’Ganga 2’) when grown in refined sand at three levels of boron, deficient (0.0033 mg L−1), normal (0.33 mg L−1) and excess (3.3 mg L−1), each at three levels of phosphorus, deficient (0.17 m mol L−1), normal (1.5 mmol L−1) and excess (4 m mol L−1). The effects of phosphorus deficiency (i.e., reduction in dry matter, soluble protein, DNA, activity of ribonuclease and increase in the activities of peroxidase, acid phosphatase and polyphenol oxidase) were intensified by a combined deficiency of boron and phosphorus. The effects of boron deficiency (i.e., reduction in dry weight, leaf boron and DNA and increase in starch content and in the activities of starch phosphorylase, peroxidase and polyphenol oxidase) become more intense in the treatment-deficient B-excess P. The decreases caused by excess phosphorus (i.e., dry weight, starch and sugar content, DNA, RNA and activity of ribonuclease) were aggravated by combined excess of boron and phosphorus.Key words: Zea mays, maize, boron and phosphorus nutrition

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34

Liu, Yongfeng, Zhiyi Chen, T. B. Ng, Jie Zhang, Mingguo Zhou, Fuping Song, Fan Lu, and Youzhou Liu. "Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916." Peptides 28, no. 3 (March 2007): 553–59. http://dx.doi.org/10.1016/j.peptides.2006.10.009.

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35

Papaphilis, A. D., E. F. Kamper, C. Kattamis, and G. A. Pangalis. "Activity of ribonuclease H in cells of chronic B-lymphocytic leukaemia: correlation with clinical stage." British Journal of Haematology 70, no. 3 (November 1988): 301–6. http://dx.doi.org/10.1111/j.1365-2141.1988.tb02486.x.

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36

Zapun, André, Nigel J. Darby, Daniel C. Tessier, Marek Michalak, John J. M. Bergeron, and David Y. Thomas. "Enhanced Catalysis of Ribonuclease B Folding by the Interaction of Calnexin or Calreticulin with ERp57." Journal of Biological Chemistry 273, no. 11 (March 13, 1998): 6009–12. http://dx.doi.org/10.1074/jbc.273.11.6009.

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37

Fu, Daotian, Ling Chen, and Roger A. O'Neill. "A detailed structural characterization of ribonuclease B oligosaccharides by 1H NMR spectroscopy and mass spectrometry." Carbohydrate Research 261, no. 2 (August 1994): 173–86. http://dx.doi.org/10.1016/0008-6215(94)84015-6.

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38

Condon, Ciarán, Jordi Rourera, Dominique Brechemier-Baey, and Harald Putzer. "Ribonuclease M5 Has Few, If Any, mRNA Substrates in Bacillus subtilis." Journal of Bacteriology 184, no. 10 (May 15, 2002): 2845–49. http://dx.doi.org/10.1128/jb.184.10.2845-2849.2002.

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ABSTRACT In Bacillus subtilis, maturation of 5S rRNA is catalyzed by an enzyme called RNase M5. We searched for potential mRNA substrates for RNase M5 by gene array technology, based on the premise that most endonucleolytic cleavages have an effect on the stability of RNA and hence on steady-state levels of expression. Only a handful of genes had significantly altered expression in rnmV mutants compared to wild-type strains that could subsequently be confirmed by Northern blotting. The effect of RNase M5 on the expression of the best candidates, the odhAB and sucCD operons, is indirect, by a mechanism we do not yet understand. We show that an effect of RNase M5 on the expression of the remaining candidate, ctsR, is due to the failure to process the 5S rRNA contained in the rrnW lying directly upstream. We thus conclude that RNase M5 has very few or possibly no mRNA substrates in B. subtilis.

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39

Aburatani, Hiroyuki, Akiyo Matsumoto, Takashi Ishikawa, Fumimaro Takaku, and Hiroshige Itakura. "Single Base Substitution between Human Intestinal and Hepatic Apolipoprotein B mRNA Detected by Ribonuclease Cleavage Analysis." Journal of Biochemistry 105, no. 6 (June 1989): 911–15. http://dx.doi.org/10.1093/oxfordjournals.jbchem.a122778.

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40

Gotte, Giovanni, Massimo Libonati, and Douglas V. Laurents. "Glycosylation and Specific Deamidation of Ribonuclease B Affect the Formation of Three-dimensional Domain-swapped Oligomers." Journal of Biological Chemistry 278, no. 47 (September 8, 2003): 46241–51. http://dx.doi.org/10.1074/jbc.m308470200.

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41

Fang, X., S. Wong, and B. F. Mitchell. "Messenger RNA for progesterone receptor isoforms in the late-gestation rat uterus." American Journal of Physiology-Endocrinology and Metabolism 283, no. 6 (December 1, 2002): E1167—E1172. http://dx.doi.org/10.1152/ajpendo.00116.2002.

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The progesterone receptor (PR) has three isoforms, PR-A, PR-B, and PR-C, which have different physiological effects. PR-A may inhibit PR-B-mediated transcription. Parturition requires withdrawal of progesterone (P4). This could occur through decreased P4 concentrations and/or a change in PR isoforms to diminish the effect of P4. We measured mRNA for PR isoforms in rat uterine tissues through late gestation and investigated the effects of antagonists to estrogen (tamoxifen) and P4 (RU-486). Two specific probes were used for ribonuclease protection assays; one (PR-total) measured PR-A, PR-B, and PR-C, and the other recognized only PR-B. PR-total mRNA increased significantly through late gestation, whereas PR-B was unchanged. The ratio of PR-total to PR-B peaked on the day before parturition. Tamoxifen delayed parturition and inhibited the increase in PR-total without affecting PR-B mRNA. RU-486 caused early parturition associated with increased PR-total mRNA, with no change in PR-B. We conclude that there are significant changes in PR isoforms in late-gestation rat uterus. These changes may be regulated by estrogen and P4and may influence the timing of parturition.

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Rosewicz, S., L. D. Lewis, X. Y. Wang, R. A. Liddle, and C. D. Logsdon. "Pancreatic digestive enzyme gene expression: effects of CCK and soybean trypsin inhibitor." American Journal of Physiology-Gastrointestinal and Liver Physiology 256, no. 4 (April 1, 1989): G733—G738. http://dx.doi.org/10.1152/ajpgi.1989.256.4.g733.

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Regulation of pancreatic gene expression by cholecystokinin (CCK) was examined in the rat using cloned cDNA probes to quantify changes in specific mRNAs (amylase, trypsinogen I, chymotrypsinogen B, and ribonuclease). Rats were administered intraduodenally an elemental liquid diet. Plasma CCK levels were raised to levels comparable to physiological postprandial levels either by intraduodenal perfusion with soybean trypsin inhibitor (SBTI) (6.9 +/- 1.0 pM, n = 8) or by continuous intravenous infusion with cholecystokinin octapeptide (CCK-8, 6.0 +/- 0.9 pM, n = 6). SBTI infusion resulted in fivefold increases in trypsinogen I and chymotrypsinogen B mRNA levels after 48 h. In contrast SBTI infusion had no effect on amylase mRNA levels and led to a decrease in ribonuclease mRNA levels to approximately 50% of control after 48 h. Intravenous infusion with CCK-8 for 24 h resulted in plasma levels of CCK comparable to those obtained with SBTI and had similar effects on digestive enzyme mRNA levels. These data suggested that SBTI acted via its ability to raise plasma CCK levels. To further test the specificity of these effects, animals were infused intraduodenally with the specific CCK receptor antagonist L364,718. Although the antagonist itself had no effect on digestive enzyme mRNA levels, antagonist treatment totally abolished the effects of both CCK infusion and SBTI treatment. These data therefore indicate that CCK regulates digestive enzyme gene expression at plasma concentrations comparable to physiological postprandial levels. Furthermore, the ability of SBTI infusion to increase plasma CCK accounts for its effects on pancreatic digestive enzyme mRNA levels.

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Kikuchi, Kaeko, Yoshio Taniyama, and Ryuji Marumoto. "Evaluation of the 2 NH2A—T Pair in Hybridization, I Synthesis of the DNA/RNA Hybrid Oligomers Containing 2-Aminoadenosines." Zeitschrift für Naturforschung B 43, no. 5 (May 1, 1988): 623–30. http://dx.doi.org/10.1515/znb-1988-0523.

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Abstract DNA decamers containing 2-aminoadenosine were synthesized. Oligonucleotide duplexes including the 2 NH2A-T base pairs were prepared and their Tm profile examined. Contrary to expectation, elevation of the Tm value by the 2 NH2 group is very small in DNA/RNA duplexes. From the CD spectra measurement, we assume that the distortion of the B-DNA structure caused by scattered DNA/RNA base pairing diminishes the efficient hydrogen bonding and base stacking of the duplexes. It was also found that the DNA duplexes containing 2-aminoadenosine hybrids are considerably resistant to ribonuclease T2 or nuclease P1 digestion.

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FUKUYAMA, Yuko, Yoshinao WADA, Yuzo YAMAZAKI, Noriyuki OJIMA, Masaki YAMADA, and Koichi TANAKA. "A New Analytical Method for Glycoprotein Structure Analysis Using MALDI-QIT-TOFMS: An Application to Ribonuclease B." Journal of the Mass Spectrometry Society of Japan 52, no. 6 (2004): 328–38. http://dx.doi.org/10.5702/massspec.52.328.

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Unverzagt, Carlo. "Building blocks for glycoproteins: Synthesis of the ribonuclease B fragment 21–25 containing an undecasaccharide N-glycan." Tetrahedron Letters 38, no. 32 (August 1997): 5627–30. http://dx.doi.org/10.1016/s0040-4039(97)01278-1.

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JOAO, Heidi C., and Raymond A. DWEK. "Effects of glycosylation on protein structure and dynamics in ribonuclease B and some of its individual glycoforms." European Journal of Biochemistry 218, no. 1 (November 1993): 239–44. http://dx.doi.org/10.1111/j.1432-1033.1993.tb18370.x.

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Reid, Gavin E., James L. Stephenson,, and Scott A. McLuckey. "Tandem Mass Spectrometry of Ribonuclease A and B: N-Linked Glycosylation Site Analysis of Whole Protein Ions." Analytical Chemistry 74, no. 3 (February 2002): 577–83. http://dx.doi.org/10.1021/ac015618l.

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Mock, K., J. Firth, and J. S. Cottrell. "Application of on-line HPLC-FAB mass spectrometry to the analysis of enzymatic digests of ribonuclease B." Organic Mass Spectrometry 24, no. 8 (August 1989): 591–96. http://dx.doi.org/10.1002/oms.1210240813.

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González, Leandro, Marta Bruix, Teresa Díaz-Mauriño, Ten Feizi, Manuel Rico, Dolores Solís, and Jesús Jiménez-Barbero. "Conformational Studies of the Man8 Oligosaccharide on Native Ribonuclease B and on the Reduced and Denatured Protein." Archives of Biochemistry and Biophysics 383, no. 1 (November 2000): 17–27. http://dx.doi.org/10.1006/abbi.2000.2031.

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Villa, Juan Antonio, Daniel P. Pike, Kunjan B. Patel, Elena Lomonosova, Gaofeng Lu, Roz Abdulqader, and John E. Tavis. "Purification and enzymatic characterization of the hepatitis B virus ribonuclease H, a new target for antiviral inhibitors." Antiviral Research 132 (August 2016): 186–95. http://dx.doi.org/10.1016/j.antiviral.2016.06.005.

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