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Dissertations / Theses on the topic 'Ribozym'

Author: Grafiati
Published: 4 June 2021
Last updated: 27 January 2023

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1

Frauendorf, Christian. "Entwicklung eines Selektionsschemas zur Isolierung katalytischer Ribonucleinsäuren und eines dynamischen Verfahrens zur Bestimmung von Konzentrationen kleiner organischer Moleküle." [S.l. : s.n.], 2000. http://www.diss.fu-berlin.de/2001/11/index.html.

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2

Schlatterer, Jörg Christian. "Funktionalisierte Nukleinsäuren Studien & Selektion von Ribozymen /." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968649416.

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3

Lamberth, Stefanie. "Bestimmung konformationeller und struktureller Eigenschaften mit NMR-Spektroskopie Synthese von Dolastatin-10-Derivaten, Hammerhead-Ribozym und Phospholamban /." [S.l.] : [s.n.], 2001. http://zaurak.tm.informatik.uni-frankfurt.de/diss/data/src/00000175/00000175.ps.gz.

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4

Schultz, Martin. "Entwicklung eines metabolisch stabilen Ribozyms gegen die mRNA des Parathormon-verwandten Proteins (PTHrP)." Doctoral thesis, [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962880841.

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5

Welz, Rüdiger. "Entwicklung eines Twinribozyms für die RNA-Reparatur." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2003. http://dx.doi.org/10.18452/14943.

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Ziel dieser Arbeit war die Entwicklung eines Twinribozyms, das durch zweifache Spaltung und anschließende zweifache Ligation den Fragmentaustausch innerhalb einer RNA-Substratsequenz katalysiert. Als Basis für dieses Konzept diente das Hairpinribozym, das durch die Einführung einer zusätzlichen Helix derart verändert wurde, dass die Verknüpfung zweier Einheiten in einem Twinribozym möglich wurde. Weitere Sequenz- und Strukturoptimierung führte zu zwei Einzelmotiven mit hoher Substratselektivität und verbesserter Kofaktorakzeptanz. Durch die Verwendung von 5-Benzylmercapto-1H-tetrazol als Akti
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6

Lobitz, Stephan [Verfasser]. "Posttranskriptionelle Expressionshemmung von ETV6/RUNX1 durch lentivirale Transduktion mit dem Ribozym buRz28 / Stephan Lobitz." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2012. http://d-nb.info/1030381100/34.

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7

Materna, Verena Waltraut. "Bedeutung des ABC-Transporters MRP2/cMOAT/ABCC2 bei der Cisplatinresistenz humaner Tumorzellen." Doctoral thesis, [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=96607078X.

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8

Meyer, Mélanie. "Contributions to the study of the architecture and evolution of ribozymes." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-01063838.

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NcRNA represent most of primary transcripts RNA in higher eukaryotes and tune gene expression via diverse mechanisms. They adopt 3D structures composed at 70% by WC bp forming A-form helices linked by RNA motifs. We identified the pk-turn, a new RNA motif related to k-turns that allow for the formation of a bend of 60° between stems P16 and P17 from the bacterial RNaseP. Yet it features different sequence and structural requirements than k-turns. The 2nd ribozyme which got my attention is the LCrz inserted in GIR2, a group I intron. This twintron is observed in the pre-rRNA 18S of the small su
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9

Wang, Ji. "Développement d'une méthode SELEX pour l'identification de ribozymes pour l'aminoacylation et analyse d’ARN aminoacylés dans le transcriptome d'Escherichia coli." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS269/document.

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Les ribozymes sont des ARN naturels ou artificiels possédant une activité catalytique. Les ribozymes artificiels ont été identifiés in vitro par la méthode SELEX, et plusieurs d'entre eux ont été caractérisés par des études cinétiques. Ces molécules sont impliquées dans des réactions de clivage, de ligation, de modification d'extrémités d'ARN, de polymérisation, de phosphorylation et d'activation de groupements acyl. Parce qu'elle est nécessaire à la traduction, l'aminoacylation des ARN joue un rôle évolutif important dans la transition du monde de l'ARN vers le monde moderne de l'ADN et des p
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10

Roy, Snigdha. "Cleavage and Ligation Studies in Hairpin and Hammerhead Ribozymes Using Site Specific Nucleotide Modifications." ScholarWorks @ UVM, 2008. http://scholarworks.uvm.edu/graddis/203.

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RNA catalysis is of fundamental importance in many biological functions, such as the peptidyl transferase activity of the ribosome and genetic control by riboswitches, among others. Small ribozymes are a convenient system to increase our understanding about the structure, folding and catalytic mechanism of ribozymes. This dissertation includes analysis of certain aspects of the catalytic mechanism in the hairpin and hammerhead ribozyme. In the hairpin ribozyme, we studied the functional consequences of molecular substitutions at two conserved positions, A9 and A10. These nucleotides are
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11

Niazi, Adnan Khan. "Régulation et coordination rétrograde de l'expression génétique mitochondriale." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-00940588.

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Le système génétique complexe des mitochondries de plantes supérieures n'a pu être étudié par des approches transgéniques car les méthodes conventionnelles ne permettent pas de transformer ces organites. Une approche alternative a été développée au laboratoire, grâce à l'existence d'un processus naturel assurant l'import d'ARN de transfert (ARNt) du cytosol dans les mitochondries. Il a été montré qu'un mime d'ARNt peut servir in vivo de navette pour importer dans les mitochondries de plante des ARN-passagers exprimés à partir de transgènes nucléaires. L'utilisation d'un transribozyme comme séq
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12

Kulikov, Katharina [Verfasser]. "Charakterisierung des CPEB3 Ribozyms : Studien zur Aufklärung des Mechanismus eines humanen Ribozyms / Katharina Kulikov." Bonn : Universitäts- und Landesbibliothek Bonn, 2019. http://d-nb.info/1185576134/34.

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13

Wurmthaler, Lena Amelie [Verfasser]. "Influence of ribozyme cleavage in E. coli expression systems and the utilization of ligand-dependent ribozymes for conditional genetic control in C. elegans / Lena Amelie Wurmthaler." Konstanz : KOPS Universität Konstanz, 2018. http://d-nb.info/1219852635/34.

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14

Chartrand, Pascal. "Études cinétiques et structurales d'un ribozyme en tête de marteau et d'un ribozyme dépendant du plomb." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0008/NQ35578.pdf.

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15

Müller, Dirk. "Ribozyme zur Genregulation in Aspergillus giganteus." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974042633.

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16

Lafontaine, Daniel. "Étude structurale du ribozyme antigénomique delta." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0018/NQ57005.pdf.

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17

Johnson, Ashley Kirtley. "GROUP I INTRON-DERIVED RIBOZYME REACTIONS." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_diss/286.

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Group I introns are catalytic RNAs capable of self-splicing out of RNA transcripts. Ribozymes derived from these group I introns are used to explore the molecular recognition properties involved in intron catalysis. New ribozyme reactions are designed based on the inherent ability of these ribozymes to perform site-specific nucleophilic attacks. This study explores the molecular recognition properties of group I intron-derived ribozyme reactions and describe a new ribozyme reaction involving molecular recognition properties previously not seen.We report the development, analysis, and use of a
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18

Lafontaine, M. Daniel. "Étude structurale du ribozyme antigénomique delta." Thèse, Université de Sherbrooke, 1999. http://savoirs.usherbrooke.ca/handle/11143/4125.

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Le virus de l'hépatite delta (VHD) est un virus satellite du virus de l'hépatite B (VHB). Chez les humains, la co-infection de VHD avec VHB, ou la super-infection des individus porteurs de VHB, augmente la sévérité de la maladie. Le VHD possède un génome ARN circulaire simple brin d'environ 1700 nucléotides dont la structure secondaire n?est probablement pas branchée puisque le génome possède une haute autocomplémentarité. La progéniture du virus est assurée par le mécanisme de la réplication en double cercle roulant. La réplication engendre des multimères linéaires génomiques et antigénomique
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19

Donahue, Christine Patricia. "Intracellular Hairpin Ribozyme Catalysis: a Dissertation." eScholarship@UMMS, 1999. https://escholarship.umassmed.edu/gsbs_diss/172.

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Ribozymes are catalytic RNA molecules capable of performing functions normally attributed to proteins. The hairpin ribozyme is derived from the (-) strand of the satellite RNA of Tobacco Ringspot Virus, where it functions in processing rolling circle transcription intermediates. The hairpin ribozyme catalyzes the breaking of a phosphodiester bond to form a 2'-3' cyclic phosphate and a 5' OH on the product termini. RNA substrates are recognized through Watson Crick base pairs. In theory, ribozymes are able to cleave any RNA that forms the correct secondary structure. Therefore, ribozymes have b
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20

Lafontaine, M. Daniel. "Étude structurale du ribozyme antigénomique delta." Sherbrooke : Université de Sherbrooke, 1999.

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21

Sanders, Jane F. "Circularisation reactions of the Tetrahymena ribozyme." Thesis, University of Bath, 1992. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305527.

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22

Lemerle, E. "Les acides ribonucleiques catalytiques." Strasbourg 1, 1991. http://www.theses.fr/1991STR15039.

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23

Lage, Hermann. "Atypische pleiotrope Zytostatikaresistenz (Multidrug-Resistenz) humaner Tumorzellen." Doctoral thesis, [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963803921.

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24

Saran, Dayal. "Novel phosphotransferase ribozyme with ATPgammaS hydrolase activity." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3215205.

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Thesis (Ph. D.)--Indiana University, Dept. of Chemistry, 2006.<br>Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1995. Adviser: Donald H. Burke. "Title from dissertation home page (viewed June 20, 2007)."
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25

Buck, Amy Havens. "Protein activation of the RNase P ribozyme." Diss., Connect to online resource, 2005. http://wwwlib.umi.com/dissertations/fullcit/3178337.

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26

Twomey, Ciara. "Ribozyme delivery into the 32Db3a2 cell line." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323247.

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27

Kovacheva, Yana Stefanova. "Mechanistic studies of the Neurospora VS ribozyme." Thesis, University of Sheffield, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419622.

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28

Fiola, Karine. "Structure, mécanisme et applications du ribozyme delta." Thèse, Université de Sherbrooke, 2005. http://savoirs.usherbrooke.ca/handle/11143/4206.

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Le virus de l'hépatite delta humaine possède un génome d'ARN circulaire simple brin de 1,7 kb. Durant sa réplication en cercle roulant, la coupure des copies multimériques en monomères est assurée par la présence de motifs autocatalytiques sur la molécule ( cis ). À partir de la séquence du motif autocatalytique antigénomique, il a été possible de générer un système où le ribozyme delta (domaine catalytique) a la capacité de couper un substrat en trans. L'activité catalytique des acides ribonucléiques (ARN) lui est assurée par sa structure tridimensionnelle dynamique. Les groupements 2'-OH son
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29

Robertson, Michael Paul. "Engineered regulation of an RNA ligase ribozyme." Access restricted to users with UT Austin EID Full text (PDF) from UMI/Dissertation Abstracts International, 2001. http://wwwlib.umi.com/cr/utexas/fullcit?p3035968.

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30

Lawrence, Michael S. (Michael Scott) 1975. "[RNA polymerase ribozymes]." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/31193.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2005.<br>Title supplied by cataloger from abstract.<br>Includes bibliographical references.<br>The RNA World is a hypothetical ancient evolutionary era during which RNA was both genome and catalyst. During that time, RNA was the only kind of enzyme yet in existence, and one of its chief duties was the replication of RNA. This scenario presupposes that among all possible RNA sequences, there exist RNA replicase ribozymes, capable of synthesizing RNA using the information in an RNA template. The goal of the present work is
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31

Blaustein, Josh Edmund. "In vitro selection of a novel ligase ribozyme /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2004. http://uclibs.org/PID/11984.

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32

Bravo, Catherine. "Structure tridimensionnelle en solution d'un ribozyme antigenomique du virus de l'hepatite delta et d'un ribozyme en tete de marteau anti-hiv." Paris 11, 1996. http://www.theses.fr/1996PA11T010.

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33

Rouleau, Samuel. "Oligonucléotides comme modulateurs de l'expression génique." Thèse, Université de Sherbrooke, 2017. http://hdl.handle.net/11143/11570.

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L’ARN est sans aucun doute la molécule biologique la plus versatile qui soit. Tout comme l’ADN, il peut contenir et transmettre de l’information génétique. Tout comme les protéines, il peut accomplir une multitude de fonctions biologiques. De plus, son rôle le plus connu demeure celui d’intermédiaire entre l’ADN et les protéines. L’ARN est donc au cœur d’un bon nombre de processus biologiques. Ceci lui confère un immense potentiel thérapeutique qui jusqu’à présent demeure largement inexploité. Pour accomplir ses fonctions, l’ARN doit adopter une structure tridimensionnelle précise qui est dépe
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34

Buckelew, Aurelie Lina. "Investigation of metal-ion binding in the four-way junction construct of the hairpin ribozyme." Texas A&M University, 2003. http://hdl.handle.net/1969.1/2307.

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The hairpin ribozyme is a small catalytic RNA that cleaves a phosphodiester bond. In order for cleavage to occur, the hairpin ribozyme must properly fold into its docked conformation, in which the two loops interact to form the active site. Metal ions and the four-way junction play critical roles in the stabilization of the docked conformation. The work presented in this thesis attempts to investigate the metal-ion dependence of the docking of the four-way junction construct of the hairpin ribozyme. In addition, the activity of the hairpin ribozyme in the presence of Mn2+ was observed. Initial
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35

Ward, William, and William Ward. "Metal Ion-Mediated Folding and Catalysis of the Hammerhead Ribozyme." Thesis, University of Oregon, 2012. http://hdl.handle.net/1794/12375.

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The factors that determine RNA structure formation, stability, and dynamics are inexorably linked to RNA function. The Hammerhead ribozyme (HHRz) has long served as a model for studying metal-dependent folding and catalysis in RNA. The HHRz consists of three helices meeting at a common junction of conserved nucleotides that form the active site of the ribozyme. Current models of metal-dependent HHRz function involve a requirement for divalent metals to globally fold the ribozyme at low metal concentrations, followed by a second metal-dependent process which activates the HHRz for catalysis. Th
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36

Hotchkiss, Graham. "Towards ribozyme-mediated gene therapy of HIV-1 infections /." Stockholm, 2000. http://diss.kib.ki.se/2000/91-628-4007-X/.

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37

Boots, Jennifer Lynn. "Structural and functional studies of the natural hammerhead ribozyme." Connect to online resource, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3273656.

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38

Deschênes, Patrick. "Compréhension des composantes régissant la spécificité du ribozyme delta." Mémoire, Université de Sherbrooke, 2003. http://savoirs.usherbrooke.ca/handle/11143/3303.

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Le virus de l'hépatite delta humaine (VHD) est un pathogène infectieux qui est associé à une hépatite fulminante chez l'humain. Ce virus possède un génome circulaire d'ARN simple brin comportant deux régions auto-catalytiques (ribozymes). Ce présent travail à [i.e. a] pour but de mieux comprendre la spécificité de substrat pour la coupure du ribozyme delta . À partir de la séquence du type sauvage, une collection de substrats mutants, aux positions -1 à -4 du site de coupure, a été synthétisée. La caractérisation de ces substrats a été effectuée par cinétique enzymatique et diverses constantes
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39

Harris, Lorena Beatriz. "Characterization of a Small Ribozyme with Self-Splicing Activity." Bowling Green State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1225668492.

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40

Harris, Lorena B. ""Characterization of a small ribozyme with self-splicing activity"." Bowling Green, Ohio : Bowling Green State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1225668492.

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41

Deschênes, Patrick. "Compréhension des composantes régissant la spécificité du ribozyme delta." Sherbrooke : Université de Sherbrooke, 2003.

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42

Márquez, Viter. "Switching off the mechanism for maintaining the Ribosomal reading frame: translational regulation of release factor 2." [S.l.] : [s.n.], 2003. http://www.diss.fu-berlin.de/2003/223/index.html.

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43

Zirwes, Oliver. "Einfluss von Mutationen in der Leader-Region eines ribosomalen RNA-Operons auf die Faltungskinetik und Assemblierung der kleinen ribosomalen Untereinheit." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962712132.

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44

Többen, Udo. "A novel function for the eukaryotic translation elongation factor 1A." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972338101.

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45

Özlem, Tastan Ayse. "How lazy are the tRNAs on the ribosome? new insights for the [alpha]-e [alpha-epsilon] model /." [S.l. : s.n.], 2003. http://www.diss.fu-berlin.de/2003/224/index.html.

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46

Le, Dû Sylvain. "Développement d'outils pour la production de ribozymes efficaces au niveau des plantes." Sherbrooke : Université de Sherbrooke, 2001.

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47

Xu, Jiacui [Verfasser]. "Ribozymes for Aminoacylation / Jiacui Xu." Greifswald : Universitätsbibliothek Greifswald, 2012. http://d-nb.info/1023614863/34.

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48

Hudson, Andrew J. "Cellular delivery of hammerhead ribozymes." Thesis, Aston University, 1997. http://publications.aston.ac.uk/12352/.

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Site-specific chemical modification of hammerhead ribozymes was evaluated as a means of enhancing biological stability. Chimeric, 2'-O-methylated ribozymes, containing only five unmodified ribonucleotides, were catalytically active in vitro (kcat = 1.46 min-1) and were significantly more stable in serum and lysosomal enzymes than unmodified (all-RNA) counterparts. Furthermore, they remained undegraded in cell-containing media for up to 8 hours. Stability enhancement allowed cellular uptake properties of radiolabelled ribozymes to be assessed following exogenous delivery. Studies in vulval and
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49

Thorne, N. M. H. "(ADP-ribosyl)ation and Ap4A." Thesis, University of Bath, 1988. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383797.

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50

Vogt, Matthew John. "Spectroscopic investigation of metal-RNA interactions." Texas A&M University, 2004. http://hdl.handle.net/1969.1/1344.

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Metal-RNA interactions are important to neutralize the negative charge and aid in correctly folding the RNA. Spectroscopically active metal ions, especially Mn2+, have been used to probe the type of interaction the metal has with RNA. In previous studies, the hammerhead ribozyme, an RNA motif that catalyzes a site-specific phosphodiester bond cleavage reaction, was determined by room temperature EPR (electron paramagnetic resonance) studies to have a set of tightly and weakly bound metal ions. Under high salt concentrations, the hammerhead was found to bind a single Mn2+ ion with high affini
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