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1
Kumar, Vipin, Rashmi Nigam, Raju ., Yachna Gupta, and Gyan Manjri Rao. "Blast disease of basmati rice and its management." International Journal of Agricultural Invention 2, no. 01 (June 30, 2017): 87–91. http://dx.doi.org/10.46492/ijai/2017.2.1.20.
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Basmati rice is an important staple food grain crop in the world. Basmati rice is an important export commodity among the food grains. The biotic and abiotic factors are adversely affected the Basmati rice but Basmati rice blast caused by Pyricularia oryzae Cavara (synonym Pyricularia grisea Sacc). The anamorph of Magnaporthe grisea (Herbert), is one of the most destructive and wide spread diseases as compared to other diseases of Basmati rice. It causes leaf blast, neck blast and panicle blast of paddy. This disease generally causes yield loss of 10-20 percent but in severe cases yield loss may reach up to 80 percent. Management of blast disease using healthy seed, resistance varieties, many biological controls like as T. harzianum, T. viride and P. fluorescens @10g/kg by seed treatment and foliar spray. The chemical control by fungicides such as carbendazim, tricyclazole, isoprothilane, tebuconazole, hexaconazole reduced leaf but not neck blast; on the contrary, tricyclazole was effective against neck blast and panicle blast.
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Greer, C. A., and R. K. Webster. "Occurrence, Distribution, Epidemiology, Cultivar Reaction, and Management of Rice Blast Disease in California." Plant Disease 85, no. 10 (October 2001): 1096–102. http://dx.doi.org/10.1094/pdis.2001.85.10.1096.
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Rice blast, caused by Pyricularia grisea, was first found in California in 1996. Disease surveys have shown the blast disease is spreading at a moderate rate in California rice fields. Although no effective major resistance genes are known to occur in widely grown commercial California cultivars, there appear to be differences among the cultivars with respect to field susceptibility to the pathogen. P. grisea was recovered from rice crop residue and commercial seedlots which are suggested as possible sources of initial P. grisea inoculum in California rice fields. Examination of weather data indicates that environmental conditions in California rice-producing areas are permissive for rice blast but generally not optimal for epidemic development. Spore trapping determined that the majority of P. grisea conidia are generally not released until approximately 6:00 A.M. and would not have sufficient time for infection before leaf wetness periods end. Azoxystrobin showed positive results with respect to reduction of neck blast incidence and yield increases in small-plot and large-scale fungicide trials.
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Santoso, Santoso, Anggiani Nasution, and Nani Yunani. "KERAGAMAN DAN SUMBER GEN KETAHANAN VARIETAS PADI LOKAL TERHADAP PATOGEN Pyricularia grisea PENYEBAB PENYAKIT BLAS." Jurnal Ilmu-Ilmu Pertanian Indonesia 22, no. 2 (December 3, 2020): 119–28. http://dx.doi.org/10.31186/jipi.22.2.119-128.
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[DIVERSITY AND THE SOURCE OF RESISTANCE GENE OF LOCAL RICE VARIETIES ON THE PATHOGENIC OF Pyricularia grisea CAUSE OF BLAST DISEASE]. Local rice varieties are known to have resistance or source of genes to pests even though their productivity yield is low. The pathogen of Pyricularia grisea is a cause of blast disease, which is one of the obstacles in rice production. The research aims to characterize the resistance of local rice varieties to the pathogen of P. grisea and to evaluate the virulence level of P. grisea pathogens against local rice varieties. A total of 100 local rice varieties and check varieties are susceptible and resistant namely Kencana Bali and Situ Patenggang tested their resistance to 4 dominant pathogenic of P. grisea i.e. races 033, 073, 133 and 173. Inoculation was carried out on stages 4-5 leaves or 18-21 days after seedling in a green house. The results showed a high genetic diversity of local rice varieties against pathogenic races 033, 073, 133 and 173. Based on the response of local rice varieties resistance i.e. moderately resistant (MR), resistant (R) and susceptible (S) to pathogenic races 033, 073 , 133 and 173 obtained 45 resistance response patterns. Cere Bereum varieties which are local rice varieties from West Java and Situ Patenggang resistant check varieties have a resistant response to 4 P. grisea pathogenic races used. A number of local rice varieties also show a resistant and moderately resistant response to the four pathogenic races used include Siam 11, Pare Siriendah, Menyan, Cere Manggu and Enud-Rawa Bogo. Local rice varieties Djedah and Padi Hitam (2) are local rice varieties that have a specific response of resistant or moderately resistant to race 173. Race 133 and 173 have higher virulence rates than those of races 033 and 073 on local rice varieties. The results of this study indicate that there is a great potential for the utilization of local rice varieties, as a source of resistance genes for blast disease for the assembly of rice varieties that are resistant to blast disease.
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Zuraidah, Zuraidah, and Sri Wahyuni. "Uji Daya Hambat Beberapa Ekstrak Bahan Alami Terhadap Pertumbuhan Cendawan Pyricularia grisea." BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan 6, no. 2 (November 28, 2019): 81. http://dx.doi.org/10.22373/biotik.v6i2.5611.
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Pyricularia grisea is a pathogenic fungus which causing rice blast disease that affected leaf, stems and rice panicles of rice plant. A possible option to reduce the use of synthetic chemical fungicide in managing the blast disease is by using plant natural active ingredients to inhibit the mycelial growth of Pyricularia grisea. This research evaluated the potential uses of bay leaf, tropical almond leaf, and kaffir lime leaf extract in inhibiting pathogenic fungus Pyricularia grisea. A Completely Randomized Design (CRD) with 3 replications and 4 treatments were used in this research. Work procedures included leaf extracts preparation, pathogenic fungi rejuvenation, growth media preparation and isolates plantation on PDA media. The parameter in this study was the diameter of the Pyricularia grisea inhibition zone. Data was analysed by analysis of variance (ANOVA) at 95% confidence interval with Duncan Multiple Range Test (DMRT) at 5% level (α = 0.05) as post hoc test if significant differences between treatments were detected using SPSS 16.0. The results of this study indicated that the highest concentration (100%) of each plant leaf extract was able to inhibit the mycelial growth of the pathogenic fungus Pyricularia grisea. The zone of inhibition of bay leaf extract was 1.77 mm, tropical almond leaf extract was 2.33 mm and lime leaf extract was 1.52 mm. The largest diameter of the inhibition zone was found on media with tropical almond leaf extract.
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PRABHU, ANNE S., ELCIO P. GUIMARÃES, MARTA C. FILIPPI, LEILA G. ARAUJO, and VERIDIANO A. CUTRIM. "Expression of resistance in rice hybrids to Pyricularia grisea." Fitopatologia Brasileira 27, no. 5 (September 2002): 454–60. http://dx.doi.org/10.1590/s0100-41582002000500003.
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Thirty-nine rice (Oryza sativa) hybrids and their restorers were assessed for vertical resistance to Pyricularia grisea in the rice blast nursery, and in artificial inoculation tests with two pathotypes, under controlled greenhouse conditions. The hybrids were developed from cytoplasmic genetic male sterile lines 046I and IR 58025A, derived from WA cytoplasm. In the rice blast nursery all hybrids showed susceptible reaction varying from 5 to 9. Compatible and incompatible leaf blast reactions of hybrids to two pathotypes, IC-1 and IB-45, were observed in inoculation tests. A majority of the hybrids were resistant when the restorer was resistant. However, seven of the 25 F1 hybrids exhibited susceptible reactions even when one of the parents was resistant to a pathotype. The partial resistance of 11 hybrids and their parents that showed compatible reactions to two pathotypes was analyzed. Differential interaction between isolates and genotypes was observed for partial resistance in relation to both disease severity and lesion number indicating the specific nature of partial resistance.
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Greer, C. A., S. C. Scardaci, and R. K. Webster. "First Report of Rice Blast Caused by Pyricularia grisea in California." Plant Disease 81, no. 9 (September 1997): 1094. http://dx.doi.org/10.1094/pdis.1997.81.9.1094a.
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Rice blast, caused by Pyricularia grisea (Cooke) Sacc., generally recognized as the most important disease of rice (Oryzae sativa L.) worldwide, was first found in the Sacramento Valley of California in 1996. Symptoms observed in commercial fields during September and October consisted mainly of darkened lesions at the panicle neck node and flag leaf collar. Many of the panicles with neck rot were partially filled or blank. Disease foci were irregular within fields and the most severely affected areas had approximately 50% of the panicle necks with symptoms. Initial identification of P. grisea was made by isolating the fungus from panicle neck nodes and leaf collars on potato dextrose agar. Subsequent identifications were made by placing symptomatic panicle neck nodes or leaf collars on moist filter paper in petri dishes to allow sporulation of the fungus. P. grisea was consistently recovered from these tissues and was identified by conidial morphology. A survey of over 500 rice fields in five Sacramento Valley counties was conducted in September and October 1996. Rice blast was confirmed in 33 commercial fields, which are spread over an area of approximately 460 km2 in Glenn County (27 fields) and northern Colusa County (6 fields). No blast was found in Butte, Sutter, or Yolo counties. P. grisea was recovered from cvs. M-201, M-202, M-204, M-103, M-401, S-102, L-204, and Calmochi-101 and several proprietary lines. To confirm pathogenicity of P. grisea, cv. M-201 was inoculated in the greenhouse 28 days after seeding with a suspension of 20,000 conidia per ml of sterile deionized water, covered with plastic bags for 2 days, and evaluated 7 days later. P. grisea isolates from cvs. M-201, M-202, and M-204 produced typical leaf and collar blast symptoms on inoculated plants, whereas control plants remained asymptomatic. P. grisea was consistently recovered from lesions on inoculated plants. No rice cultivars currently grown in California are known to have resistance to blast.
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Puri, KD, SM Shrestha, KD Joshi, and GB KC. "Reaction of Different Rice Lines Against Leaf and Neck Blast under Field Condition Of Chitwan Valley." Journal of the Institute of Agriculture and Animal Science 27 (May 1, 2006): 37–44. http://dx.doi.org/10.3126/jiaas.v27i0.693.
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The severity of the rice blast disease (Pyricularia grisea) of both leaf and neck varies with different environment and it becomes destructive under favorable condition. The leaf and neck blast resistance and susceptible interaction of 30 different tropical rice lines were evaluated under low-, mid- and up-land conditions of Chitwan district and classified on the basis of disease severity with respect to susceptible check, Masuli. Of them, 5, 10, 12 and 3 rice lines were resistant to leaf blast, moderately resistant, moderately susceptible susceptible, respectively. Similarly, for the neck blast nine lines were resistant, thirteen moderately resistant, seven moderately susceptible and one was susceptible. The progenies from Masuli/MT4 had the highest leaf and neck blast susceptible reaction, while the most of progenies from IPB (Irradiated Pusa Basmati), KalinghaIII_IR64, Radha 32_ KIII and Masuli_IR64 were resistant, and the most promising sources against leaf and neck blast resistance. Therefore, the progenies from these parents can be used in breeding the resistant variety.
Key words: Pyricularia grisea, resistance, rice lines
J. Inst. Agric. Anim. Sci. 27:37-44 (2006)
8
Rossman, Amy Y., Richard J. Howard, and Barbara Valent. "Pyricularia grisea, the Correct Name for the Rice Blast Disease Fungus." Mycologia 82, no. 4 (July 1990): 509. http://dx.doi.org/10.2307/3760024.
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Varaprasada Rao, Ch, and P. Anil Kumar. "Integrated Disease Management of Rice Blast Caused by Pyricularia grisea (Sacc.)." International Journal of Current Microbiology and Applied Sciences 7, no. 03 (March 10, 2018): 2952–58. http://dx.doi.org/10.20546/ijcmas.2018.703.341.
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Rossman, Amy Y., Richard J. Howard, and Barbara Valent. "Pyricularia Grisea the Correct Name for the Rice Blast Disease Fungus." Mycologia 82, no. 4 (July 1990): 509–12. http://dx.doi.org/10.1080/00275514.1990.12025916.
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Guerber, C., and D. O. TeBeest. "Infection of Rice Seed Grown in Arkansas by Pyricularia grisea and Transmission to Seedlings in the Field." Plant Disease 90, no. 2 (February 2006): 170–76. http://dx.doi.org/10.1094/pd-90-0170.
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Rice blast, caused by Pyricularia grisea, is an important and serious disease of rice (Oryza sativa) in the southeastern United States. The disease sporadically reaches epidemic proportions on susceptible cultivars within fields and over large areas within Arkansas. The main overwintering sources of inoculum reportedly include infected rice stubble, related host species, and infected seed. The objectives of the research were to (i) determine whether rice seed grown in Arkansas were infected with P. grisea, (ii) investigate the relationship between seed infection and seedling disease, and (iii) determine if planting naturally infected seed could lead to the subsequent development of rice blast on seedlings in the field. The results of seed assays showed that P. grisea was detected in samples of foundation, certified, and production seed. Estimated levels of infection by P. grisea of rice seed from 66 samples of rice seed grown in Arkansas ranged from 0 to 10.5%. Planting infected seed in the greenhouse and the field resulted in seedling infection. Planting naturally infected seed may result in disease development (i) from seedlings grown from infected seed planted beneath the soil surface, (ii) from seedlings grown from germinating seed left on the soil surface, (iii) from seed coats, or (iv) from nongerminated seed left on the soil surface after planting. Additional research is necessary to establish the mechanisms of infection of seedlings and to establish disease thresholds for this important fungal pathogen of rice.
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Varma, Yamini CK, and P. Santhakumari. "Biointensive management of blast disease of rice." Oryza-An International Journal on Rice 58, no. 2 (June 30, 2021): 317–36. http://dx.doi.org/10.35709/ory.2021.58.2.8.
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Blast of rice caused by Pyricularia grisea (Cooke.) Sacc. (Teleomorph :Magnaporthe oryzae) is a serious disease of rice and causes considerable reduction in yield. Overdose and untimely application of chemical fungicides disturb the rice ecosystem, pollute the environment and induce resistant mutants of the pathogen. An ecofriendly integrated disease management programme should be implemented to avoid overuse of a single control method and fight against genetic resistance. Salicylic acid (0.01ml/ l) and Benzoic acid (0.01ml/ l) proved superior in inducing disease resistance among different inducers tested at Regional Agricultural Research Station, Pattambi, Palakkdad district, under Kerala Agricultural University under green house conditions. As an integrated approach, Palmarosa oil + Carbendazim and Palmarosa oil+ Salicylic acid were the most effective treatments .Compatibility studies under in vitro conditions showed that Trichoderma harzianum and fluorescent pseudomonad were compatible to each other, and also palmarosa oil and Neemazal were compatible with Trichoderma harzianum and Fluorescent pseudomonad. For the management of blast disease under field conditions with high B:C ratio, Palmarosa oil (0.1%) + Carbendazim (0.1%)was the best treatment followed by Trichoderma (2%) + Fluorescent pseudomonad (2%)+ Palmarosa oil (0.1%).
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Dutta, Suman, Sekhar Bandyopadhyay, and Sushmita Jha. "Cultural and Morphological Characterization of Pyricularia grisea Causing Blast Disease of Rice." International Journal of Current Microbiology and Applied Sciences 8, no. 09 (September 10, 2019): 514–22. http://dx.doi.org/10.20546/ijcmas.2019.809.062.
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Long, D. H., J. C. Correll, F. N. Lee, and D. O. TeBeest. "Rice Blast Epidemics Initiated by Infested Rice Grain on the Soil Surface." Plant Disease 85, no. 6 (June 2001): 612–16. http://dx.doi.org/10.1094/pdis.2001.85.6.612.
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Field experiments were conducted in 1996 and 1997 with a marked strain of Pyricularia grisea to determine if inoculum from infested rice grain could cause primary infections and sustain a rice blast epidemic during the growing season by giving rise to leaf, collar, and neck symptoms. The marked strain, a sulfate nonutilizing (sul) mutant of P. grisea, was grown on autoclaved rice seed for 7 days at 25°C. Infested rice grains were applied to the soil surface at the time of plant emergence (approximately 10 days after planting) at densities of 0, 0.5, 5, 25, and 50 grains per 0.1 m2 in plots planted to the blast susceptible cv. M-201. Leaf blast symptoms were first detected in the plots containing infested grain 35 days after plant emergence in both 1996 and 1997. The sul mutant was isolated from more than 90% of the lesions sampled from rice seedlings 35 to 45 days after plant emergence. Leaf blast increased more rapidly in plots with 25 and 50 infested grains per 0.1 m2 than in plots with less inoculum pressure (0.5 and 25 infested grains per 0.1 m2), although in 1996, leaf blast incidence recorded at midseason in plots containing 0.5 and 5 infested grains per 0.1 m2 was 41 and 55%, respectively. At the end of both seasons, the sul mutant was recovered from over 90% of the leaf, collar, and neck blast lesions except for one sample date in 1996. Rice blast was not detected in the control plots (no infested grain) in 1997 and not until 65 days after planting in 1996. Comparisons of disease progress on leaves between the marked strain and the parental wild-type strain under field conditions indicated that development of disease caused by the sul mutant was similar to disease caused by the wild-type strain.
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Jo, Young-Ki, Guo-Liang Wang, and Michael J. Boehm. "Expression Analysis of Rice Defense-Related Genes in Turfgrass in Response to Magnaporthe grisea." Phytopathology® 97, no. 2 (February 2007): 170–78. http://dx.doi.org/10.1094/phyto-97-2-0170.
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Magnaporthe grisea (anamorph = Pyricularia grisea) causes blast on rice (Oryza sativa) and gray leaf spot on turfgrass. Gray leaf spot is a serious disease on St. Augustinegrass (Stenotaphrum secundatum), perennial ryegrass (Lolium perenne), and tall fescue (Festuca arundinacea). Virulence assays performed in this study revealed that M. grisea collected from rice could also cause disease on St. Augustinegrass and tall fescue. One rice isolate, Che86061, caused similar disease reactions on susceptible cultivars of rice and St. Augustinegrass and an incompatible interaction on resistant cultivars of both species. To explore whether similar defense-related genes are expressed in rice and St. Augustinegrass, a rice cDNA library was screened using pooled cDNAs derived from M. grisea-infected St. Augustinegrass. Thirty rice EST (expressed sequence tag) clones showing differential expression in St. Augustinegrass following M. grisea inoculation were identified and classified into six putative functional groups. Northern blot analyses of seven EST clones that collectively represented each putative functional group confirmed that the expression of five out of seven EST clones was similar in both rice and St. Augustinegrass. This study represents one of the first attempts to use a broad-scale genomic approach and resources of a model monocot system to study defense gene expression in St. Augustinegrass following M. grisea infection.
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Leung, Hei, and Paul H. Williams. "Genetic analyses of electrophoretic enzyme variants, mating type, and hermaphroditism in Pyricularia oryzae Cavara." Canadian Journal of Genetics and Cytology 27, no. 6 (December 1, 1985): 697–704. http://dx.doi.org/10.1139/g85-104.
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Pyricularia oryzae (teleomorph: Magnaporthe grisea) parasitizes a variety of gramineous hosts and causes the rice blast disease worldwide. Through matings among P. oryzae isolates from rice, finger millet, and weeping lovegrass the inheritance of electrophoretic variants of six enzymes, phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), glycerate-2-dehydrogenase (G2DH), malate dehydrogenase-3 (MDH-3), lactate dehydrogenase-1 (LDH-1), and lactate dehydrogenase-3 (LDH-3) was determined. All six variants were under single gene control as determined by tetrad and random spore analysis. However, at Ldh-3 and Mdh-3, there were consistent excesses of variant alleles among ascospore segregants. Preliminary data on the genetic control of hermaphroditism suggested that maleness in two Japanese rice isolates might be due to a single gene mutation. Linkage analyses among the six electrophoretic markers, mating type, and hermaphroditism suggested loose linkage between Pgm and G2dh with a recombination frequencies of 43.0%.Key words: linkage, Magnaporthe grisea, rice blast fungus.
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Magar, Prem Bahadur, Basistha Acharya, and Bishnu Pandey. "Use of Chemical Fungicides for the Management of Rice Blast (Pyricularia Grisea) Disease at Jyotinagar, Chitwan, Nepal." International Journal of Applied Sciences and Biotechnology 3, no. 3 (September 25, 2015): 474–78. http://dx.doi.org/10.3126/ijasbt.v3i3.13287.
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Rice blast caused by Pyricularia grisea Sacc. is the important disease of rice and different fungicides against this disease were evaluated in summer 2014 at Karma Research and Development Center, Jyotinagar, Chitwan, Nepal. A susceptible rice cultivar ‘Mansuli’ was planted in randomized complete block design and fungicides viz. Tricyclazole 22% + Hexaconazole 3% SC (0.2%), Streptomycin 5% + Thiophanate Methyl 50% WP (0.15%), Prochloraz 25% EC (0.3%), Kasugamycin 2% WP (0.2%), Hexaconazole 4% + Zineb 68 % WP (0.2%) and Udaan (Hexaconazole 3% SC) (0.2%) were sprayed thrice at weekly interval starting from the booting stage. All these fungicides were found to be effective in controlling leaf and neck blast disease as compare to control one. Among them, Tricyclazole 22% + Hexaconazole 3% SC was found to be the most effective with least leaf blast severity (6.23%), neck blast incidence (8.97%), and highest percentage disease control (87.08% and 79.62% in leaf blast and neck blast respectively) and grain yield (4.23 t/ha) followed by Prochloraz 25% EC (0.3%) and Udaan (Hexaconazole 3% SC) (0.2%). It is therefore concluded that Tricyclazole 22% + Hexaconazole 3% SC fungicide could be used to control rice blast at weekly interval starting from the booting stage for three times. Int J Appl Sci Biotechnol, Vol 3(3): 474-478
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Chen, H. L., B. T. Chen, D. P. Zhang, Y. F. Xie, and Qifa Zhang. "Pathotypes of Pyricularia grisea in Rice Fields of Central and Southern China." Plant Disease 85, no. 8 (August 2001): 843–50. http://dx.doi.org/10.1094/pdis.2001.85.8.843.
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Blast, caused by the fungal pathogen Pyricularia grisea, is the most devastating disease of rice worldwide. Knowledge of the pathotype composition of the pathogen in rice fields is essential for rational deployment of resistance genes in rice breeding programs. In this study, we assayed the pathotypes of the pathogen populations using samples recently collected from 13 major rice-growing provinces of central and southern China. In all, 792 single spore isolates were tested for pathogenicity reactions using 13 host differentials consisting of six indica and seven japonica near-isogenic lines (NILs). The compositions of the pathogen populations were complex; 48 pathotypes were identified with the indica NILs, 82 pathotypes were detected with the japonica NILs, and a total of 344 pathotypes were identified with both indica and japonica NILs. There were large differences in distribution of the pathotypes among the different rice-growing areas. Even neighbor provinces seemed to differ sharply in types and frequencies of the most prevalent pathotypes. There was also a large difference in the frequencies of the isolates producing compatible reactions on the NILs, indicating the difference in frequencies of avirulence genes in the pathogen populations. The data provided very useful information for formulating strategies for improving blast resistance in rice breeding programs.
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Toan, Nguyen Phu, Pham Thi Thu Ha, and Tran Dang Xuan. "Effects of Rice Blast Fungus (Pyricularia grisea) on Phenolics, Flavonoids, Antioxidant Capacity in Rice (Oryza sativa L.)." International Letters of Natural Sciences 61 (January 2017): 1–7. http://dx.doi.org/10.18052/www.scipress.com/ilns.61.1.
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Rice blast fungus (Pyricularia grisea) is one of the most problematic pathogen to significantly reduce rice production worldwide. In this study, after being inoculated withP. grisea, changes in phenolic components and antioxidant capacity and correlation with the resistant level against rice blast fungus were investigated. Among screened rice cultivars, AV-3 was the strongest resistant, whereas BII-3 was the most susceptible. It was found that although total contents of phenolics and flavonoids, and antioxidant capacities varied among studied varieties, no significant coefficient with the resistance againstP. griseawas observed. After rice was affected by rice blast fungus, total phenolics and flavonoids were markedly reduced, but in contrast, the DPPH scavenging activities of only the susceptible rice cultivars was reduced. Among the 11 phenolic acids detected, catechol was found only in the tolerant cultivar AV-3, whereas the amount of cinnamic acid was increased after infection. Quantity of vanillin was also promoted, except in the susceptible cultivar BII-3 that was significantly reduced. Findings of this study showed that the resistant level againstP. griseawas proportionally correlated to the antioxidant capacity. Catechol, cinnamic acid, and vanillin may play a role but it needs further elaboration. Observations of this study suggested that the infection of blast disease by reducing amount of phenolics and flavonoids that may weaken the resistance of rice against this detrimental fungus.
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Lestari, Shyntiya Ayu, Umi Kalsum, and Evan Purnama Ramdan. "Efikasi Beberapa Agens Hayati Terhadap Penekanan Pertumbuhan Pyricularia grisea Secara In Vitro." Agrosains : Jurnal Penelitian Agronomi 23, no. 1 (April 1, 2021): 31. http://dx.doi.org/10.20961/agsjpa.v23i1.48174.
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<p>The demand for rice as an important food crop in Indonesia is still constrained by the attack of blast disease caused by <em>Pyricularia grisea</em>. Controlling using synthetic chemical pesticides has a negative impact on the environment so that the use of biological agents is an alternative option. This study aims to determine the effectiveness of several biological agents against the pathogen <em>P. grisea</em> that causes blast disease in rice plants in vitro. The study used a completely randomized design (CRD) with 6 levels of treatment and was repeated 3 times using the dooble culture method. The results of the study showed that the inhibition of <em>P. grisea</em> was the highest in the treatment of <em>P. grisea</em> × <em>Trichoderma</em> sp. and <em>P. grisea</em> × <em>Gliocladium</em> sp. namely 67.04% and 51.85% compared to other treatments. The <em>P. polymyxa</em> and <em>P. fluorescence</em> treatments showed low inhibition, namely 23.70% and 28.89%. Biological agents <em>Trichoderma</em> sp. and <em>Gliocladium</em> sp. able to inhibit the growth of the fungus <em>P. grisea</em>. Each biological agent has a different percentage of inhibition in that caused by the inhibitory mechanism of the biological agent.</p>
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Groth, D. E. "Azoxystrobin Rate and Timing Effects on Rice Head Blast Incidence and Rice Grain and Milling Yields." Plant Disease 90, no. 8 (August 2006): 1055–58. http://dx.doi.org/10.1094/pd-90-1055.
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Growing blast susceptible rice (Oryza sativa) cultivars often requires farmers to use fungicides to prevent significant reductions in rice grain and milling yields. Studies were conducted to determine the optimum rate and rice growth stage for single or multiple applications of azoxystrobin to control blast (Pyricularia grisea). Azoxystrobin was applied foliarly to naturally infected field plots in 2001 to 2005 at rates of 0.11, 0.17, and 0.22 kg a.i. ha-1 at boot (B) and heading (H) or only at H growth stages, and at 0.17 kg a.i. ha-1 at 5 (H+5), 10 (H+10), and 15 (H+15) days after H and B with low or high blast pressure. Head blast incidence (percent heads infected) was assessed 1 to 2 weeks before harvest. A fungicide application made at H, H+5, and B+H significantly reduced blast incidence with high and low disease pressure, resulting in significantly higher grain and head rice milling yields compared with unsprayed plots with high blast pressure. There were no significant effects of fungicide rate on blast development or yield following the H, B+H, and H+5 applications. With fungicide applications made at B, H+10, and H+15 days postheading, rice had higher disease incidence, resulting in lower grain and milling yields compared with rice receiving a heading application.
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Khadka, Ram B., Sundar M. Shrestha, Hira K. Manandhar, and Gopal BKC. "Study on Differential Response of Pyricularia grisea Isolates from Rice, Finger Millet and Panicum sp. with Local and Alien Media, and Their Host Range." Nepal Journal of Science and Technology 13, no. 2 (March 8, 2013): 7–14. http://dx.doi.org/10.3126/njst.v13i2.7707.
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Blast (Pyricularia grisea) is an economically important disease of rice and finger millet in Nepal. Isolates of the fungus from different hosts differed in their response in media for mycelial growth and sporulation. Radial mycelial growth (RMG) and days of sporulation (DOS) of P. grisea were studied by culturing three fungal isolates from rice, finger millet and Panicum sp. on six different media: prune agar (PA), oat meal agar (OMA), potato dextrose agar (PDA), finger millet leaf decoction agar (FLDA), finger millet polish agar (FPA) and finger millet meal agar (FMA). The highest RMG was found in the isolates from finger millet and the lowest in the isolates from rice. The shortest DOS (1 week) was found in the isolate from rice and the longest (>2 weeks) in the isolate from finger millet. Among the different media used, PA and OMA were found to be the best for mycelial growth and sporulation of the isolates both from rice and finger millet. The shape, color and compactness of the fungal colonies varied with the media and isolates used. Cross inoculation studies showed that the fungus isolates from rice were able to infect all the plant species (rice, finger millet, Panicum sp., Eleusine indica and Setaria sp.) while isolates from finger millet were only able to infect three plant species (E. coracana, Setaria sp. and E. indica). This shows that the weed management is more important in finger millet fields than in rice field to manage the blast disease; and growing of rice adjacent to finger millet field is dangerous for blast epidemics in finger millet since rice serves as the source of inoculums. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 7-14 DOI: http://dx.doi.org/10.3126/njst.v13i2.7707
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Lai, X. H., M. A. Marchetti, and H. D. Petersen. "Comparative Slow-Blasting in Rice Grown Under Upland and Flooded Blast Nursery Culture." Plant Disease 83, no. 7 (July 1999): 681–84. http://dx.doi.org/10.1094/pdis.1999.83.7.681.
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Evaluation of rice for resistance to rice blast disease caused by Pyricularia grisea usually is conducted in upland (nonflooded) disease nurseries, although all commercial U.S. rice is produced under flood irrigation. Upland rice is more susceptible to leaf blast than is flooded rice, and the magnitude of this differential susceptibility can vary among cultivars. This 2-year study was undertaken to determine (i) the relationship between rates of disease development (slow-blasting) in upland and flooded rice and (ii) the value to a rice breeding program of establishing a flooded blast nursery, a facility far more difficult to manage than an upland nursery. Among 200 rice lines compared for leaf blast susceptibility under upland and flooded cultures, only 7 were rated as slightly more resistant under upland culture and 136 rated more resistant under flooded culture. Disease ratings under upland and flooded cultures were highly correlated (R = 0.819). Among 14 preselected cultivars over 2 years, disease development curves under upland and flooded cultures were highly correlated (R = 0.990). The cultivars with intermediate susceptibility under upland culture appeared to benefit most from flooded culture. Upland culture provided more opportunities to assess slow-blasting than did flooded culture, since many lines that produced susceptible-type lesions in upland culture failed to do so under flooded culture. It was concluded that adequate information on comparative leaf blast resistance among rice lines was attainable from upland blast nurseries and that routine evaluation of rice breeding lines for blast resistance in flooded nurseries was not necessary.
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Prabhu, Anne Sitarama, Emílio da Maia de Castro, Leila Garcês de Araújo, and Rodrigo Fascin Berni. "Resistance spectra of six elite breeding lines of upland rice to Pyricularia grisea." Pesquisa Agropecuária Brasileira 38, no. 2 (February 2003): 203–10. http://dx.doi.org/10.1590/s0100-204x2003000200006.
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The objective of this work was to evaluate the resistance spectra of six elite breeding lines of rice, developed for improved yield and grain quality, in inoculation tests in the greenhouse and in the field. Forty-six isolates of Pyricularia grisea collected from the cultivar Primavera, 31 from the cultivar Maravilha and 19 from six elite breeding lines, totaling 96 were utilized for inoculations. Out of 11 international and 15 Brazilian pathotypes, IC-1, IB-9, and BD-16, respectively, were identified as most frequent isolates collected from the cultivar Primavera. The isolates retrieved from Maravilha belong to four international and 11 Brazilian pathotypes, the predominant ones being IB-9 and IB-49 and BB-1 and BB-21, respectively. Lines CNAs 8711 and CNAs 8983 showed resistant reaction to all test isolates from Maravilha, while CNAs 8983 was susceptible to three isolates of Primavera pertaining to the pathotype IC-1. A majority of isolates exhibiting compatible reaction to Primavera were incompatible to Maravilha and vice-versa.Field assessment of rice blast utilizing the area under disease progress curve as a criterion for measuring disease severity showed significant differences among the six breeding lines. The isolates of P. grisea exhibiting differential reaction on breeding lines can be utilized in pyramiding resistance genes in new upland rice cultivars.
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Reges, Juliana Teodora de Assis, Matheus Mereb Negrisoli, Adriano Francis Dorigan, Vanina Lilián Castroagudín, João Leodato Nunes Maciel, and Paulo Cezar Ceresini. "Pyricularia pennisetigena and P. zingibericola from invasive grasses infect signal grass, barley and wheat." Pesquisa Agropecuária Tropical 46, no. 2 (June 2016): 206–14. http://dx.doi.org/10.1590/1983-40632016v4641335.
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ABSTRACT Fungal species from the Pyricularia genus are associated with blast disease in plants from the Poaceae family, causing losses in economically important crops such as rice, oat, rye, barley, wheat and triticale. This study aimed at characterizing the pathogenicity spectrum of P. pennisetigena and P. zingibericola to signal grass, barley and wheat, as well as comparing them with those from the species P. grisea and P. oryzae pathotype Triticum, which occur widely in the Brazilian agroecosystem. Twenty isolates of Pyricularia spp. were obtained from infected leaf samples of invasive plant species from wheat fields. The isolates classification into distinct Pyricularia species was done using molecular phylogeny based on actin and calmodulin genes. Pyricularia pennisetigena and P. zingibericola inoculated on plant leaves, at a concentration adjusted to 105 conidia mL-1, were pathogenic to signal grass, barley and wheat, with varying levels of aggressiveness.
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Viji, G., B. Wu, S. Kang, W. Uddin, and D. R. Huff. "Pyricularia grisea Causing Gray Leaf Spot of Perennial Ryegrass Turf: Population Structure and Host Specificity." Plant Disease 85, no. 8 (August 2001): 817–26. http://dx.doi.org/10.1094/pdis.2001.85.8.817.
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Gray leaf spot is a serious disease of perennial ryegrass (Lolium perenne) turf in the United States. Isolates of Pyricularia grisea causing the disease in perennial ryegrass were characterized using molecular markers and pathogenicity assays on various gramineous hosts. Genetic relationships among perennial ryegrass isolates were determined using different types of trans-posons as probes. Phylogenetic analysis using Pot2 and MGR586 probes, analyzed with AMOVA (analysis of molecular variance), showed that these isolates from perennial ryegrass consist of three closely related lineages. All the isolates belonged to a single mating type, MAT1-2. Among 20 isolates from 16 host species other than perennial ryegrass, only the isolates from wheat (Triticum aestivum) and triticale (× Triticosecale), showed notable similarity to the perennial ryegrass isolates based on their Pot2 fingerprints. The copy number and fingerprints of Pot2 and MGR586 in isolates of P. grisea from perennial ryegrass indicate that they are genetically distinct from the isolates derived from rice (Oryza sativa) in the United States. The perennial ryegrass isolates also had the same sequence in the internal transcribed spacer (ITS) region of the genes encoding ribosomal RNA as that of the wheat and triticale isolates, and exhibited rice isolate sequence polymorphisms. In pathogenicity assays, all the isolates of P. grisea from Legacy II perennial ryegrass caused characteristic blast symptoms on Marilee soft white winter wheat, Bennett hard red winter wheat, Era soft white spring wheat, and Presto triticale, and they were highly virulent on these hosts. An isolate from wheat and one from triticale (from Brazil) were also highly virulent on perennial ryegrass and Rebel III tall fescue (Festuca arundinacea). None of the isolates from perennial ryegrass caused the disease on Lagrue rice, and vice versa. Understanding the population structure of P. grisea isolates infecting perennial ryegrass and their relatedness to isolates from other gramineous hosts may aid in identifying alternate hosts for this pathogen.
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Pradhan, Tensirani, MK Mishra, Sandhyarani Nanda, and Anamita Sen. "Physiological and nutritional variability of different Pyricularia grisea isolates isolated from various hosts inciting blast disease of rice." International Journal of Chemical Studies 9, no. 1 (January 1, 2021): 3165–67. http://dx.doi.org/10.22271/chemi.2021.v9.i1ar.11716.
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Xiong, Lizhong, Min-Woo Lee, Min Qi, and Yinong Yang. "Identification of Defense-Related Rice Genes by Suppression Subtractive Hybridization and Differential Screening." Molecular Plant-Microbe Interactions® 14, no. 5 (May 2001): 685–92. http://dx.doi.org/10.1094/mpmi.2001.14.5.685.
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Identification of host genes involved in defense responses is one of most critical steps leading to the elucidation of disease resistance mechanisms in plants. In this study, two different cloning strategies were employed to identify defense-related genes from a tropical japonica rice cultivar (Oryza sativa cv. Drew). With the use of bacterial colony arrays, differential screening of a blast fungus (Pyricularia grisea)-induced rice cDNA library led to the isolation of 22 distinct rice genes that are expressed differentially in response to blast infection. Sequence analysis indicates that most of them are full-length cDNAs encoding pathogenesis-related proteins or other relatively abundant proteins. In combination with treatments of cycloheximide plus jasmonic acid (JA) or benzothiadiazole (BTH) in rice seedlings, the polymerase chain reaction-based suppression subtractive hybridization also was conducted to search for immediate early (IE) defense-related genes whose transcription is independent of de novo protein synthesis. The initial screening of only 768 subtracted clones resulted in the identification of 34 distinct IE genes that are induced by JA, BTH, and/or blast infection. Database searches revealed that these IE genes encode putative mitogen-activated protein kinase, diacylglycerol kinase, zinc finger protein, RelA-SpoT protein, ankyrin-containing protein, ABC transporter, β-ketoacyl-CoA synthase, and other potential defense-signaling components. Further characterization of these novel IE genes will likely facilitate the elucidation of defense signal transduction in rice plants.
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Thinlay, R. S. Zeigler, and M. R. Finckh. "Pathogenic Variability of Pyricularia grisea from the High- and Mid-Elevation Zones of Bhutan." Phytopathology® 90, no. 6 (June 2000): 621–28. http://dx.doi.org/10.1094/phyto.2000.90.6.621.
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Thirty isolates of P. griseacollected from rice during a blast epidemic in 1995 in the high (1,800 to 2,600 m) and middle (1,200 to 1,800 m) elevations of Bhutan and 80 isolates collected from one rice cultivar from two high- and two mid-elevation sites in 1996 were analyzed for virulence. Differential varieties were indica CO39, with five near-isogenic lines (NILs) for resistance genes in the genetic background of CO39, and japonica Lijiangxintuanheigu (LTH), with five NILs for LTH. Twelve selected Bhutanese landraces also were studied. In addition, 10 blast nurseries consisting of the NIL sets, important local landraces, and representatives of international differential groups were established in the 1996 and 1997 growing seasons in the mid- and high-elevation agroecological zones. The 110 isolates were differentiated into 53 pathotypes based on the 2 NIL sets. Thirteen isolates were avirulent on all of the NILs but were compatible with some landraces. Several isolates were able to attack one of the NILs of CO39 but not CO39. These results strongly suggest that both CO39 and LTH possess previously unidentified resistance. The landraces were not uniform in their reactions to the isolates. When a reaction index taking into account all individual plant reactions was used, isolates that had been assigned to the same pathotype could be further differentiated, indicating that the NIL sets could not completely discriminate virulences in Bhutanese P. grisea populations. In the trap nurseries, disease was always present in the middle elevations, but disease was very low during July 1996 in the high elevations and only present during August and September 1997. Almost all varietal groups were more frequently attacked in the middle than in the high elevations, indicating that the virulence spectrum is wider and the conduciveness of the environment is greater in the middle elevations. Landraces from the high elevations were most susceptible, followed by international differential groups 7 and 8. The results suggest that selection has yielded landraces with more complete and complex resistance in the more disease-conducive mid-elevation environment. At the same time, the pathogen population also possesses a wider virulence spectrum in that environment.
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WINDARSIH, GUT, and DWINITA WIKAN UTAMI. "Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field." Nusantara Bioscience 9, no. 4 (November 2, 2017): 371–77. http://dx.doi.org/10.13057/nusbiosci/n090406.
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Windarsih G, Utami DW. 2017. Evaluation of neck blast resistance and agronomical performances on double haploid rice population in greenhouse and endemic field. Nusantara Bioscience 9: 371-377. Blast disease caused by fungal Pyricularia grisea Sacc. is one of the most destructive diseases of rice in the world. The development of blast-resistant rice varieties will be essential to control this disease. This research aimed (i) to compare the resistance response to neck-blast among DH lines from double cross IR54/Parekaligolara//Bio110/Markuti and the differential varieties against three selected Indonesian blast races in greenhouse, (ii) to identify the gene(s) that caused the resistance to neck-blast based on the association between the resistance response and the genotype evaluation using molecular markers linked to Pi1, Pi33, Pib, Pir4 and Pir7 genes, and (iii) to evaluate the resistance response to leaf and neck blast on DH lines in endemic field (Sukabumi) and the agronomical performance of selected DH lines in optimum field in Ciasem of Subang, West Java, Indonesia during December 2013 to March 2014. Eleven double haploid lines from double-crossing IR54/Parekaligolara//Bio110/Markuti, the differential varieties as resistant control and the US2 variety for susceptible control were observed for neck-blast resistance response to three blast races in greenhouse and endemic field (Sukabumi), while the agronomical performances were observed in field of Ciasem-Subang. The results based on the genotyping evaluation, leaf and neck blast resistance, either in greenhouse and endemic location, and the agronomical performance in field showed that 5 selected double haploid lines had leaf and neck blast resistance and good performance on field trial. Thus they are promising for use either for further testing forwarding into releasing variety or used as donor for further blast resistant breeding activities.
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Hubert, Judith, Robert B. Mabagala, and Delphina P. Mamiro. "Efficacy of Selected Plant Extracts against <i>Pyricularia grisea</i>, Causal Agent of Rice Blast Disease." American Journal of Plant Sciences 06, no. 05 (2015): 602–11. http://dx.doi.org/10.4236/ajps.2015.65065.
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Rusli, Iqna Khayatina, Loekas Soesanto, and Ruth Feti Rahayuniati. "PENGARUH PUPUK ORGANIK CAIR DAN ASAP CAIR DALAM PENGENDALIAN Xanthomonas oryzae pv. oryzae DAN Pyricularia grisea PADA PADI GOGO GALUR G136 POTENCY OF LIQUID ORGANIC FERTILIZER AND LIQUID SMOKE TO CONTROL (Xanthomonas oryzae pv. oryzae and Pyricularia grisea IN UPLAND RICE G136 LINE)." Jurnal Perlindungan Tanaman Indonesia 20, no. 2 (February 8, 2017): 95. http://dx.doi.org/10.22146/jpti.17690.
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Increment of upland rice production in Indonesia faces many problems mainly from kresek caused by Xanthomonas oryzae pv. oryzae and blast caused by Pyricularia grisea. The use of Liquid Organic Fertilizer (LOF) and liquid smoke provides an alternative method to control the pathogen. This research aimed at knowing the potency of the leaf fertilizer applied to the soil and the liquid smoke to control the disease in G136 line’s rice and on the crop growth. Randomized Block Design was used with six treatments and replicated four times. The treatments were control without liquid smoke, control with 2% liquid smoke, using 2 ml l-1 LOF Biosena without or with 2% liquid smoke, and using 4 ml l-1 LOF Biosena without or with 2% liquid smoke. The variables observed were incubation period, disease intensity, infection rate, crop height, number of shoots, weight of 1,000 grains and rice production per hectare. The result of the research showed that treatment using the leaf LOF applied to the soil of 4 ml l-1 or 2 ml l-1, and combining with 2% liquid smoke was not able to suppress the kresek and blast development in G136 line. All treatment influenced the number of shoots and the crop height plants, but didn’t influence the weight of 1,000 grains and the rice production per hectare.Peningkatan produksi padi gogo di Indonesia menemui banyak kendala di antaranya adalah penyakit kresek yang disebabkan oleh Xanthomonas oryzae pv. oryzae dan penyakit blas yang disebabkan Pyricularia grisea. Penggunaan pupuk organik cair (POC) dan asap cair merupakan salah satu alternatif dalam pengendalian patogen ini. Penelitian bertujuan untuk mengetahui potensi POC daun yang diaplikasikan pada tanah dan asap cair dalam menekan serangan penyebab penyakit pada padi gogo galur G136 serta pengaruhnya terhadap pertumbuhan tanaman. Rancangan yang digunakan adalah Rancangan Acak Kelompok terdiri atas enam perlakuan dan empat ulangan, yaitu kontrol tanpa asap cair, kontrol menggunakan asap cair 2%, 2 ml/l POC Biosena tanpa asap cair dan menggunakan asap cair 2%, 4 ml/l POC Biosena tanpa asap cair dan menggunakan asap cair 2%. Variabel yang diamati adalah masa inkubasi, intensitas penyakit, laju infeksi, tinggi tanaman, jumlah anakan, bobot 1.000 bulir, dan produksi padi per hektar. Hasil penelitian menunjukkan bahwa perlakuan POC daun yang diaplikasikan pada tanah dengan dosis 4ml/l dan 2 ml/l, serta asap cair 2% belum mampu menekan perkembangan penyakit kresek dan blas pada tanaman padi gogo galur G136. Perlakuan berpengaruh terhadap jumlah anakan dan tinggi tanaman tetapi tidak berpengaruh terhadap bobot 1.000 bulir dan produksi padi per hektar.
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Tsukiboshi, T., I. Okabe, and K. Sugawara. "First Report of Blast of Guinea Grass Caused by Pyricularia sp. LS-Group in Japan." Plant Disease 93, no. 12 (December 2009): 1350. http://dx.doi.org/10.1094/pdis-93-12-1350c.
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Guinea grass (Panicum maximum Jacq.) is an important C-4 perennial herbage in the southern part of Japan. In February 2002, a blast disease was found on the grass cultivated on the Okinawa Islands, the southern most region of Japan. Early symptoms appeared as small, round or ellipsoid lesions on leaves. Lesions later expanded to 2 to 5 × 1 to 2 mm and were spindle shaped and grayish white in the central area with dark brown margins. We obtained three single-conidia isolates of a Pyricularia-like fungus from the lesions and deposited them in the NIAS Genebank, Japan as MAFF306662, 306671, and 306672. The isolates were grown under near-UV light on V8 juice agar for 7 days to produce conidia, and guinea grass plants of the seven- to eight-leaf stage grown from seeds in a green house, five plants for each isolate, were inoculated by atomizing them with the conidial suspension of 105 conidia/ml. The same number of plants sprayed with sterilized distilled water served as the control. The experiments were repeated twice. All plants were covered with plastic bags for 24 h at 25°C to maintain high relative humidity. After 7 days, all inoculated plants showed symptoms identical to those observed in the field. Controls remained symptom free. The Pyricularia-like fungus was reisolated from lesions on inoculated leaves. The morphologies of the isolates were observed and described from the colonies grown under the condition described above. Conidiophores were pale brown, emerging singly or in small groups, straight or flexuous, geniculate toward the apex, and 36 to 197 × 2 to 5 μm. Conidia were obpyriform, straight, colorless to pale brown, smooth, and 19 to 30 × 5 to 10 μm with two to three septa. The morphologies were the same as those of the description of the genus Pyricularia. Previously, all Pyricularia isolates from Gramineae had been identified as P. grisea, except for those from rice (3,4). However, a new taxonomy of Pyricularia spp. based on DNA analyses was proposed by Couch and Kohn (1). Only the isolates from Digitaria were classified as P. grisea and those from C-3 grasses classified as P. oryzae. However, the species names for the isolates from the other C-4 grasses were not described. We analyzed the sequences of the rDNA-ITS region (ITS1-5.8s-ITS2) and β-tubulin gene of the isolates from guinea grass following Couch and Kohn (1). The sequences of rDNA-ITS (GenBank Accession No. AB512785) and β-tubulin (AB512786) of the isolate MAFF306672 matched the sequences of those of the Pyricularia sp. LS-group (AB274426 and AB274458, respectively) isolated from Leersia oryzoides. Hirata et al. (2) reclassified Pyricularia isolates from Gramineae by multilocus phylogenetic analysis and showed that non-P. oryzae and non-P. grisea isolates could be classified into two groups of the Pyricularia sp., a LS- and a CE-group, corresponding to those isolated from Leersia spp. and Setaria spp. or Cenchrus spp. of grasses, respectively. Since no Magnaporthe teleomorph was produced by the crossing tests using the isolates, we identified the isolates from guinea grass as the Pyricularia sp. LS-group on the basis of their morphology and the molecular phylogenetic analysis. To our knowledge, this is the first report of blast on guinea grass in Japan. References: (1) B. C. Couch and L. M. Kohn. Mycologia 94:683, 2002. (2) K. Hirata et al. Mycol. Res. 111:799, 2007. (3) K. D. Hyde. Australas. Plant Pathol. 22:73, 1993. (4) R. Sprague. Diseases of Cereals and Grasses in North America. Ronald Press Company, New York, 1950.
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Utami, Dwinita Wikan, Maulidia Rahmawati, Siti Yuriyah, Ahmad Dadang, Siti Nurani, and Nurul Jadid. "Molecular Breeding for Developing A New Upland Rice Variety ‘Bio Patenggang Agritan’." Jurnal AgroBiogen 15, no. 1 (September 3, 2019): 1. http://dx.doi.org/10.21082/jbio.v15n1.2019.p1-10.
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<p>Situ Patenggang is an elite upland rice variety well-accepted by farmers, but recently this variety started to be attacked by blast disease (Pyricularia grisea) that causes yield loss up to 50%. To overcome this problem, Bio Patenggang variety has been developed through molecular breeding using blast-resistant monogenic lines as donor parents. The aim of this research was to present molecular breeding approach of Bio Patenggang development, including steps from the phenotypic selection on candidate lines to the genotypic analysis assisted by molecular markers. The phenotypic performances were assessed using Distinctness, Uniformity, and Stability (DUS) testing by following the standard method stated in the rice test guidelines. The genotypic analyses were performed using foreground and background markers. Four BC3F7 lines were selected as the candidates of the Situ Patenggang-derived variety. Based on ANOVA and Principal Component Analysis (PCA), phenotypic performances of the four lines demonstrated no significant differences with that of Situ Patenggang. The selected lines have also passed the foreground analysis confirming that the lines contained Pita, Pii, Pik-p, and Pia last resistance genes. Background analysis showed that the selected lines demonstrated agronomic perfomances very similar to that of Situ Patenggang. Association analyses showed that 14 markers were associated with the target traits and 10 out of the 14 markers were identified as co-segregation markers. The four selected lines, therefore, were proposed to be released as Situ Patenggang-derived variety. One of the lines (Sta-8-S15-TB16) has been approved to be released as a new variety, namely ‘Bio Patenggang Agritan’.</p>
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V. M., Kega, Kasina M., Olubayo F., Kamau G. M., and Nderitu J. H. "A Logit Analysis of Farmer Knowledge on Rice Blast Disease Pyricularia grisea [Cook, Sacc] at Mwea Irrigation Scheme, Kirinyaga County, Central Province, Kenya." Universal Journal of Agricultural Research 3, no. 3 (May 2015): 114–17. http://dx.doi.org/10.13189/ujar.2015.030306.
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Effendi, Kurniawan, Abdul Munif, and I. Wayan Winasa. "Survey of Rice Pests, Diseases and Natural Enemies on “Upsus” Program in Karawang District, West Java Province." Jurnal Perlindungan Tanaman Indonesia 24, no. 1 (July 7, 2020): 17. http://dx.doi.org/10.22146/jpti.50365.
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"Upsus" (Special Efforts) Program is a program to increase crop production and productivity to support the acceleration of food self-sufficiency held by the government. The targets are to increase the planting index (IP) by 0.5 and productivity by 0.3 ton/ha/Harvested Dry Grain (HDG). Increased productivity has not been reached optimally. This research aimed to determine the number/types of the dominant pests and natural enemies, and pests and diseases attack rates in the wetland rice plantation "Upsus" Program in Karawang District. Direct observation was carried out on four stages of plant development, in the nursery stage (10–14 days after sowing) 200–300 m², seedlings stage (10–20 days after planting), vegetative stage (5–6 weeks after planting), and reproductive stage (1–2 weeks after flowering) respectively within an area of 2000 m². Twenty samples were observed in the nursery stage and 50 samples in the following stages. The dominant pests and diseases found were Nilaparvata lugens, Leptocarisa oratorius, Schirpophaga incertulas, Cnaphalocrocis medinalis, Scotinophara coarctata, Mythimna separata, bacterial leaf blight (Xanthomonas. oryzae pv. oryzae), blast (Pyricularia grisea) and narrow brown spot (Cercospora oryzae). The recorded dominant natural enemies were Cyrtorhinus sp., Paederus sp., Tetragnatha sp., and Pardosa pseudoannulata. S. incertulas showed the highest attack intensity and the highest disease severity was found in bacterial leaf blight. The largest population of dominant pests and natural enemies was found in the generative stage. The high application of pesticides affected the population of pests, natural enemies, and the level of pest and disease attacks.
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Polizzi, G., I. Castello, A. M. Picco, and D. Rodino. "First Report of Gray Leaf Spot on St. Augustinegrass in Italy." Plant Disease 87, no. 12 (December 2003): 1536. http://dx.doi.org/10.1094/pdis.2003.87.12.1536a.
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St. Augustinegrass (Stenotaphrum secundatum (Walt.) Kuntze) is used for lawns in southern Italy because it is much more resistant to biotic and abiotic adversities than other turfgrass species. Because few seeds are viable, this species is established by vegetative propagation. A new disease was noticed during the spring of 2002 and 2003 on cuttings of St. Augustinegrass growing in three greenhouses in eastern Sicily. The disease affected leaves and culms and caused a progressive drying of the plants. The infection was first seen on leaves as gray, necrotic spots that enlarged in high-humidity conditions to form oval, and later, spindle-shaped lesions. In association with the lesions, it was possible to observe fungal spore development and sunken areas with blue-gray centers and slightly irregular, brown margins with yellow halos. Spots were concentrated without specific arrangement along longitudinal veins and the midrib and at the base, tip, and margins of the leaf blade. Symptoms on the culms consisted of brown-to-black blotches that sometimes extended throughout the internodes. From these infected tissues, 20 explants taken from leaves and culms were cut, washed with sterile water, and placed on 1.5% water agar (WA). Later, conidia and conidiophores were obtained from colonies with a sterile glass needle and placed on 4% WA. From these plates, two monoconidial isolates were obtained and transferred to rice meal medium (1). The colonies were identified as Pyricularia grisea Cooke (Sacc.), anamorphic state of Magnaporthe grisea (Hebert) Yeagashi & Udagawa, the cause of rice blast disease and gray leaf spot disease of turfgrasses. The conidia were pyriform to obclavate, narrowed toward the tip, rounded at the base, 2-septate, 21 to 31 μm × 6 to 10 μm (average 25.7 ×8.2 μm). Pathogenicity tests were performed by inoculating leaves and culms of six St. Augustinegrass plants with a conidial suspension of the fungus (1.5 ×105 conidia per ml). The same number of noninoculated plants was used as controls. All plants were incubated in a moist chamber with high humidity at 25°C. After 6 days, all inoculated plants showed typical symptoms of the disease. Koch's postulates were fulfilled by isolating P. grisea from inoculated plants. Gray leaf spot caused by P. grisea has been a chronic problem on St. Augustinegrass since it was first reported in 1957 (2). To our knowledge, this is the first report of P. grisea on St. Augustinegrass in Italy. While it does not appear to be an important disease in the field at this time in Sicily, it could cause losses in greenhouses where vegetative material is propagated for field planting. A preliminary molecular analysis has shown a clear distinction between the tested strain and other strains isolated from rice seeds and plants in northern Italy. References: (1) E. Roumen et al. Eur. J. Plant Pathol. 103:363, 1997. (2) L. P. Tredway et al. Plant Dis. 87:435, 2003.
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Afolabi, Clement, and Sunday Adigbo. "Effects of N Application on the Development of Blast Disease and Yield of Rice Under Sawah System in Nigeria." Annals of Tropical Research, April 14, 2014, 63–74. http://dx.doi.org/10.32945/atr3615.2014.
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Rice blast, caused by the fungus Pyricularia grisea, is one of the limiting factors in rice production causing severe crop losses under favourable environmental conditions. The effects of nitrogen applications on leaf blast development and yield of rice were studied under sawah system of production. WITA-4 and Jasmine rice varieties were planted and four levels (0, 30, 60 and 90 kg ha-1) of urea fertilizer were imposed. Nitrogen and rice varieties treatments were arranged in a split plot design with N levels as main plots and varieties as subplots, with three replications. The results obtained indicate that Jasmine variety was not infected by the blast pathogen whereas WITA-4 variety was susceptible to the disease. Disease incidence was generally high on 0 and 90 kg ha-1 but there was no significant difference among treatments. However, there was a significant difference (P=0.0001) between the N treatments for final disease severity score and the total lesion area, with 90 kg ha-1 having the highest severity score and total lesion area. Significant differences between nitrogen treatment (P=0.001), varieties (P=0.01) and the nitrogen treatment x cultivar interactions (P=0.05) with respect to grain yield were observed. In terms of yield, Jasmine variety had 5.4 tons/ha on 90 kg ha-1 N treatment but the effect of the blast was pronounced on WITA-4 variety with 4.7 tons/ha. Rice cultivation under sawah system does not preclude rice from blast infection; hence, an appropriate management system must be incorporated to ensure bumper
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PALUPI, TANTRI, and FADJAR RIYANTO. "Seed coating with biological agents to improve the quality of rice seeds contaminated with blast pathogens and increase seedling growth." Biodiversitas Journal of Biological Diversity 21, no. 2 (January 24, 2020). http://dx.doi.org/10.13057/biodiv/d210234.
Full textAbstract:
Abstract. Palupi T, Riyanto F. 2020. Seed coating with biological agents to improve the quality of rice seeds contaminated with blast pathogens and increase seedling growth. Biodiversitas 21: 683-688. The aim of this research were (1) to evaluate the compatibility of five biological agents (SP21, SP31, RP21, TP12, and TP11), (2) to evaluate the physical and physiological quality of rice seeds contaminated with Pyricularia grisea after being treated with seed coating enriched with biological agents, (3) to evaluate the effectiveness of seed coating on the incidence of blast disease and seedling growth. The first experiment tested the compatibility of five bacterial antagonists on the PSA plates. There were eight treatments in the second experiment namely seed coating enriched with SP21+SP31; SP21+RP21; SP21+TP12; SP31+RP21; RP21+TP11; fungicides; positive control (rice seeds contaminated with blast), and negative control (healthy seeds), and seven treatments in the third experiment which were the same as in experiment 2 without negative control. Parameters observed in the second experiment were: seed germination, vigor index, and growth rate, while parameters observed in the 3rd experiment were the incidence rate of blast disease and plant height. The results of experiment 1 showed that isolates SP21+SP3, SP21+RP21, SP21+TP12, SP31+RP21, and RP21+TP11 had good growth compatibility without antagonism, and they can be used as biocontrol agents. Results of experiment 2 showed that seed coating enriched with SP21+SP31 isolates has the potential to improve the physical and physiological quality of the seeds. Results of experiment 3 showed that seed coating enriched with RP21+TP11 had no incidence of blast disease up to 4 weeks after planting.
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Yuriyah, Siti, Dwinita Wikan Utami, Siti Nurani, Anggiani Nasution, Puji Lestari, Ahmad Dadang, and Suwarno Suwarno. "SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG." BERITA BIOLOGI 18, no. 1 (April 22, 2019). http://dx.doi.org/10.14203/beritabiologi.v18i1.3233.
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The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field experiment revealed that blast resistance response of the selected lines was varied from 0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance.