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Dissertations / Theses on the topic 'RNA Synthesis'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Peters, D. W. "RNA synthesis in Candida albicans." Thesis, University of Warwick, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373051.

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2

Fritz, Sarah E. "Molecular basis of the DExH-box RNA helicase RNA helicase A (RHA/DHX9) in eukaryotic protein synthesis." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437413252.

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3

Lackey, Jeremy. "New methods for the synthesis of RNA, novel RNA pro-drugs and RNA microarrays." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92290.

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The demand for synthetic oligonucleotides has grown exponentially over the past decades as genome sequencing, functional genomics, polymerase chain reaction (PCR)-based detection methods, and gene silencing via RNA interference (RNAi) consume enormous numbers of DNA and RNA oligonucleotides. Although various RNA synthesis chemistries now allow oligoribonucleotides to be produced routinely, the higher complexity and cost of RNA (over DNA) has somewhat limited its availability.<br>A major goal of this thesis work was aimed at finding ribonucleoside synthons that potentially benefit two critical

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4

Johnston, Julie Catherine. "In vitro translation of cucumber necrosis virus RNA." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/28969.

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The in vitro translation products directed by cucumber necrosis virus (CNV) RNA were analyzed in both rabbit reticulocyte lysate and wheat germ extract cell-free translation systems. In rabbit reticulocyte lysates, one major protein of ca. 33 Mr was produced. In wheat germ extracts, four proteins of ca. 41, 33, 21 and 20 Mr were produced. Hybrid-arrested translation (HART) studies using synthetic CNV antisense RNA corresponding to the entire CNV genome demonstrated that the four major proteins synthesized from CNV virion RNA in wheat germ extracts are virus-specific translation products. The g

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5

Attwater, James. "Ice as a medium for RNA-catalysed RNA synthesis and evolution." Thesis, University of Cambridge, 2011. https://www.repository.cam.ac.uk/handle/1810/246525.

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A critical event in the origin of life is thought to have been the emergence of a molecule capable of self-replication and evolution. According to the RNA World hypothesis, this could have been an RNA polymerase ribozyme capable of generating copies of itself from simple nucleotide precursors. In vitro evolution experiments have provided modern examples of such ribozymes, such as the R18 RNA polymerase ribozyme, exhibiting basic levels of this crucial catalytic activity; R18’s activity, however, falls far short of that required of an RNA replicase, leaving unanswered the question of whether RN

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6

Collis, Alana E. C. "The synthesis of vinylphosphonate-linked RNA." Thesis, University of Nottingham, 2008. http://eprints.nottingham.ac.uk/10541/.

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An introductory chapter discusses the steric block, RNase H and RNA interference antisense mechanisms and the application of antisense nucleic acids as therapeutic agents. Examples of existing chemical modifications of the sugar and backbone regions of nucleic acids are given, followed by the introduction of the vinylphosphonate modification. The vinylphosphonate has previously been examined in DNA and has been synthesised by either Pd(0) catalysed cross-coupling of an H-phosphonate with a vinyl bromide, or by the cross-metathesis of a vinylphosphonate with a terminal olefin. This thesis detai

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7

Liu, Qi. "Synthesis of small molecules targeting RNA /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3142456.

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8

D'Abramo, Claudia M. "Biochemical characterization of the BVDV RNA-dependent RNA polymerase during initiation and elongation of RNA synthesis." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111870.

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The RNA-dependent RNA polymerase (RdRp) of viruses belonging to the Flaviviridae family, including the hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV) is critical for viral replication. The major goal of this PhD study was to biochemically characterize the role of the polymerase during initiation and elongation of RNA synthesis, utilizing the BVDV RdRp as a model system. We showed that the BVDV polymerase efficiently incorporates chain-terminating nucleoside analogues, which ultimately arrest RNA synthesis. The incorporated chain-terminators, however, can be removed from the pri

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9

Repass, John F. "Studies of murine coronavirus cis-acting RNA elements that affect RNA synthesis /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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10

Gilea, Manuela Aurora. "DNA and RNA synthesis in ionic liquids." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485198.

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The solid-phase synthesis of oligonucleotide derivatives such as phosphorothioates and phosphoroselenoates was investigated. Some ionic liquids containing the trlbexyl(tetradecyl)phosphonium cation and various anions proved to be very effective in dissolving the chalcogens (sulfur and , selenium) and to prepare oligonucleoside chalcogenophosphates. The suitability ofionic liquid-based chalcogen-transfer mixtures for the synthesis of oligonucleoside chalcogenophosphates on solid-phase was evaluated and subsequently the structure-activity relationship studied in detail. The compatibility of ioni

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11

Brown, Michael Dean. "Genetic analysis of RNA splicing in the thymidylate synthase gene of bacteriophage T4." Diss., Georgia Institute of Technology, 1990. http://hdl.handle.net/1853/25390.

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12

Finnegan, Patrick Michael. "RNA synthesis in maize mitochondria : the identification of autonomously replicating RNA species and a kinetic analysis of transcript accumulation." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75931.

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Transcription in mammalian and yeast mitochondria proceeds from a few well defined promoters, with processing of polycistronic transcripts producing the mature RNAs. The levels of different sequences in the steady-state RNA populations depend on differential promoter strengths, transcription attenuation and/or selective termination, and differential RNA stabilities. To gain insights into the processes governing transcription and RNA levels in plant mitochondria, a system using isolated maize mitochondria, which synthesize bona fide mitochondrial RNAs, was developed and partially characterized

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13

Webb, Vera Ann B. "In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29448.

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The work presented explored the in vivo and in vitro synthesis of ribosomal RNA in the Gram positive, spore-forming bacterium Bacillus subtilis. The investigation began with a study of rRNA synthesis in B. subtilis during steady state growth and under nutritional shift-up conditions. The percent of transcription which is ribosomal RNA was measured by hybridization of pulse labeled RNA to a specific DNA probe carrying the 3' end of the 23S RNA gene. The fractional rate of ribosomal RNA synthesis increased with cellular growth rate, and showed a rapid increase after a nutritional shift up. RNA s

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14

Wang, Muhan. "Studies of piRNA synthesis." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:1e134b7b-4e9d-4d0e-bfd8-e4696f960ad6.

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RNA silencing is a form of post-transcriptional gene regulation, in which a small RNA guides a member of the Argonaute protein family in an effector complex to repress target gene expression. piRNAs, found in germ cells, are the most recently discovered major subset of small RNAs. A key known function of piRNA is to repress the transposable elements in the germline and maintain the germline genome integrity. The defining features of the piRNAs are 1) they are ubiquitously methylated at the 3’-end of the 2’-OH group by methyltransferase Hen1; 2) they associate exclusively with the Piwi subfamil

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15

Johnson, Moira A. "Kinetics of RNA synthesis in Rotavirus infected cells." Thesis, University of Warwick, 1988. http://wrap.warwick.ac.uk/98142/.

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A method has been developed to allow the independent quantification of the level of synthesis of each strand of different viral genes during a synchronous infection of tissue culture cells. It was developed and applied to the UK tissue culture adapted bovine Rotavirus whose genome consists of 11 discrete segments of monocistronic double stranded RNA (dsRNA). Cloned copies of the 11 genes of the UKtc Bovine Rotavirus have been sub-cloned into the polylinker region of the pGEM1 and pGEM2 transcription vectors. Using the T7 and SP6 promotors flanking this region, high specific activity RNA probes

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16

Whitfield, Julie Nicole. "Studies on a potentially prebiotic synthesis of RNA." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320683.

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17

Cook, Stephen D. "Studies on a potentially prebiotic synthesis of RNA." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267951.

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18

Takeuchi, Akiko. "RNA-protein interaction in the selenoprotein synthesis machinery." Strasbourg, 2009. http://www.theses.fr/2009STRA6054.

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La sélénocystéine est incorporée co-traductionnellement dans les sélénoprotéines en réponse à un codon UGA habituellement l’un des 3 codons stop. La protéine SBP2 joue un rôle majeur dans ce mécanisme de recodage en se liant à une structure en tige-boucle (SECIS) située dans la région 3’UTR de l’ARNm des sélénoprotéines. Nous avons isolé et caractérisé fonctionnellement SBP2 de Drosophila melanogaster. Par comparison avec SBP2 humaine, nous avons identifié un domaine de liaison à l’ARN additionnel essentiel à la liaison au SECIS et à la sous-unité ribosomique 60S et permettant une sélectivité

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19

Chen, Yuanyuan. "Characterizing RNA Structure and synthesis by Raman Microscopy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1277761094.

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20

Yunus, Muhammad Amir. "Studies on the mechanism of norovirus RNA synthesis." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9811.

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Noroviruses are common pathogens associated with gastroenteritis in humans. As single-stranded positive-sense RNA viruses, noroviruses achieve their genome replication through the synthesis of negative strand RNA intermediates that template the generation of new positive strand RNA genomes. The murine norovirus (MNV) RNA-dependent RNA polymerase (NS7) protein which is a key player in catalysing this process, has been characterised in this study. Tandem affinity purification of MNV NS7 was performed in order to identify host cell factors which interact with the NS7 protein. Proteomic analysis d

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21

Wang, Hai. "Design, synthesis and RNA binding of aminoglycoside antibiotics /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9820848.

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22

Takeuchi, Akiko Krol Alain Allmang-Cura Christine. "RNA-protein interaction in the selenoprotein synthesis machinery." Strasbourg : Université de Strasbourg, 2009. http://eprints-scd-ulp.u-strasbg.fr:8080/1133/01/TAKEUCHI_Akiko_2009.pdf.

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Thèse de doctorat : Sciences du Vivant. Aspects moléculaire et cellulaire de la Biologie : Strasbourg : 2009.<br>Thèse soutenue sur un ensemble de travaux. Titre provenant de l'écran-titre. Bibliogr. 11 p.

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23

Usman, Nassim. "Nucleoside phosphoramidites in the automated, solid phase synthesis of oligoribonucleotides and their analogues : the chemical synthesis of an E. Coli N-Formyl-Methionine tRNA." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75344.

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The use of ribonucleoside phosphoramidites protected at the 2$ sp prime$-hydroxyl with alkylsilyl ethers was developed for the solid phase synthesis of oligoribonucleotides. A series of pentadecameric oligomers of various base composition was synthesized using this methodology and a complete protocol for the deprotection of synthetic oligoribonucleotides was developed. The use of $ beta$-cyanoethyl phosphate protection was compared to that of methyl protection in the synthesis of pentadeca-uridylic acid sequences and no significant difference between the use of the two protecting groups was fo

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24

Hamilton, Andrew John. "Inhibiting gene expression with anti-sense RNA." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316938.

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25

Akhtar, Y. "Studies on the maturation pathway of ribosomal precursor RNA : Analysis of Xenopus ribosomal RNA synthesised by transcription in vitro." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382054.

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26

Jia, Yiping. "Mechanistic studies of DNA-dependent transcription initiation and RNA synthesis by bacteriophage T7 RNA polymerase /." The Ohio State University, 1998. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487953204281995.

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27

Bhutia, Pema choden. "Accuracy of TransferRNA Selection in Protein synthesis." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-162811.

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ACCURACY OF TRANSFER RNA SELECTION IN PROTEIN SY The ribosome is a rapid magnificent molecular machine that plays an important role in proteinsynthesis and it consists of RNA and protein. The 70S bacterial ribosome comprises twosubunits, 30S and 50S. The 30S small subunit of the bacterial ribosome contains a protein calledS12, encoded by the rpsL gene. The function of this S12 protein is to help arrange the mRNAcorrectly to the ribosome and to interact with transfer RNA (tRNA) to initiate translation.Mutations in the rpsL gene generate phenotypes like resistance, dependence or pseudodependence

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28

Yu, Hongchuan. "Novel Cyclo Deoxynucleoside: Synthesis and Evaluation." Thesis, Boston College, 2012. http://hdl.handle.net/2345/2751.

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Thesis advisor: Larry W. McLaughlin<br>Thesis advisor: Mary F. Roberts<br>Nucleic acids are essential biological molecules for life. For example, deoxyribonucleic acid (DNA) is the main genetic information carrier; ribonucleic acid (RNA) plays a critical role in translation and transcription. These characteristics place nucleic acids as the fundamental genetic materials of a living system. Since over a century ago, intensive attempts have been made by researchers to study the nucleic acid properties. For chemists, it is particularly interesting and important to understand the relationship betw

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29

Tetzlaff, Charles N. "Synthesis and evaluation of acylated DNA and RNA oligomers /." Thesis, Connect to Dissertations & Theses @ Tufts University, 2001.

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Thesis (Ph.D.)--Tufts University, 2001.<br>Adviser: Clemens Richert. Submitted to the Dept. of Chemistry. Includes bibliographical references (leaves 228-235). Access restricted to members of the Tufts University community. Also available via the World Wide Web;

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30

Roberts, Lisa Oriel. "Internal initiation of protein synthesis on picorna virus RNA." Thesis, University of Kent, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245654.

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31

Zhang, Ze. "The control of ribosomal RNA synthesis in mammalian cells." Thesis, University of Southampton, 2013. https://eprints.soton.ac.uk/350477/.

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The biogenesis of ribosomes is a fundamental process that occurs in all living cells. In mammalian cells, it is a highly complex process consisting of the coordinated synthesis and assembly of four ribosomal RNAs (rRNAs) with about 80 ribosomal proteins (RPs). More than 150 non-ribosomal proteins are involved in the processing of rRNAs. The main focus of this project is to use adult rat ventricular cardiomyocytes (ARVCs) as a model to address how mTOR complex 1 (mTORC1) and other signalling pathways regulate the synthesis of rRNAs. A new technique has been developed to monitor the synthesis of

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32

Hannoush, Rami Nabil. "RNA folding : synthesis, structure and biological properties of hairpins based on 2',5'-linked RNA loops." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82890.

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Ribonucleic acids (RNA) are polymers of ribonucleotides linked together by 3',5'-phosphodiester linkages and play a prominent role in protein biosynthesis. Much less common are ribonucleic acids based on 2',5'-phosphodiester linkages (2',5'-RNA). In this study, the synthesis, physical and biological properties of several 2' ,5'-linked oligoribonucleotides are described.<br>One aspect of this thesis deals with the synthesis and structural studies of 2',5'-dodecaribonucleotides. Their base sequence was designed to promote intramolecular folding by base pairing leading to the formation of

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33

Pertile, Jack James. "Synthesis of Fluorescent Promazines and Evaluation of Photophysical Properties." OpenSIUC, 2019. https://opensiuc.lib.siu.edu/theses/2524.

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A new series of fluorescent prorin (promazine-coumarin hybrid) derivatives were synthesized via piperidine catalyzed cyclization with 3-formyl-2-hydroxypromazine and ethyl acetoacetate or Meldrum’s acid. The synthesis of these new compounds focused on the introduction of electron withdrawing carbonyl groups on the 3-position of the coumarin lactone ring in order to increase polarization of the molecule which results in a longer absorbance wavelength by stabilizing intramolecular charge transfer that occurs in the excited state. The 3-acetyl derivative was further subjected to an aldol condensa

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34

York, Ashley D. "A study of viral and cellular factors in the regulation of the influenza virus RNA-dependent RNA polymerase." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:5958fafd-4c91-4434-910e-29e2dd0539b9.

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The segmented negative-sense vRNA genome of influenza A virus is replicated via a complementary RNA (cRNA) intermediate by the viral RNA-dependent RNA polymerase (RdRP). The replicative intermediate has been thought to exist as a complementary ribonucleoprotein (cRNP) complex. Development of an RNA-based affinity-purification strategy for the isolation of viral ribonucleoprotein complexes enabled the structural and functional characterisation of the previously uncharacterised cRNP complex. The cRNP exhibits a filamentous double-helical organisation with defined termini, containing the viral Rd

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35

Formanová, Nataša. "A complex synthesizing the maize mitochondrial plasmid RNA b /." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68173.

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RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in mitochondria of several maize races. The extent to which this molecule associates in vivo with proteins was investigated by both rate zonal and CsCl equilibrium density gradient centrifugations of clarified lysate of mitochondria from maize plants with the S-type cytoplasm. A soluble endogenous complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in in vitro conditions (in mitochondrial lysate), was identified. The complex had a density of 1.4

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36

Smith, Richard Wilson. "RNA metabolism and the control of protein synthesis in fish." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU089891.

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This thesis examines the regulation of protein synthesis by pre - translational events; with particular reference to the means by which RNA is able to reduce the energetic cost of protein synthesis. Protein synthesis was measured by the application of a "flooding dose" of 3H-phenylalnine. Protein synthesis rates are then calculated from the "free intracellular" and "protein bound" specific radioactivity (dpm nmole-1 phenylalanine). A similar approach was used to investigate RNA synthesis: i.e. a flooding dose of 3H-uridine. As with protein synthesis RNA synthesis rates was assessed by the rela

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37

Wang, Yang. "Chemical synthesis of DNA and RNA containing thio-substituted bases and their post-synthetic modifications." Thesis, Aston University, 2003. http://publications.aston.ac.uk/11013/.

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Modified oligonucleotides containing sulphur group have been useful tools for studies of carcinogenesis, protein or nucleic acid structures and functions, protein-nucleic acid interactions, and for antisense modulation of gene expression. One successful example has been the synthesis and study of oligodeoxynucleotides containing 6-thio-2'-deoxyguanine. 6-Thio-2'deoxyguanosine was first discovered as metabolic compound of 6- mercaptopurine (6-MP). Later, it was applied as drug to cure leukaemia. During the research of its toxicity, a method was developed to use the sulphur group as a versatile

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38

Chung, Christina. "The Saccharomyces cerevisiae nuclear cap binding complex regulates RNA synthesis and processing through interactions with RNA polymerase II." Diss., [La Jolla] : University of California, San Diego, 2009. http://wwwlib.umi.com/cr/ucsd/fullcit?p3386835.

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Thesis (Ph. D.)--University of California, San Diego, 2009.<br>Title from first page of PDF file (viewed Jan. 19. 2010). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 48-53).

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39

Greenhalgh, Duncan Alan. "Effects of 5-fluorouracil on RNA metabolism in human tumour cells." Thesis, University of Leeds, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236315.

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40

Steger, Courtney Long. "Determinants of Core Shell Dependent Rotavirus Polymerase Activity." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/87755.

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Rotaviruses (RVs) are medically significant gastrointestinal pathogens and are a leading cause of childhood mortality in many countries. The RV RNA-dependent RNA polymerase, VP1, synthesizes RNA during viral replication only in the presence of another RV protein, VP2, which comprises the innermost core shell layer of the virion. Though these VP1-VP2 interactions are essential for RV replication, the mechanism by which the core shell regulates polymerase activity remains incompletely understood. Here, we sought to identify and characterize specific regions of both VP1 and VP2 that are requir

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41

Lin, Lina. "Synthesis, Structure, Function and Biomedical Studies of Nucleic Acid Derivatized with Selenium." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/biology_diss/77.

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Nucleic acids are macromolecules in cells for storing and transferring genetic information. Moreover, nucleic acids, especially RNAs, can fold into well-defined 3D structures and catalyze biochemical reactions. As ubiquitous biological molecules in all living systems, nucleic acids are important drug targets, and they can also be used in diagnostics and therapeutics. Structural information of nucleic acids provides the foundation for DNA and RNA function studies. X-ray crystallography has been a useful tool for structural studies of bio-macromolecules at atomic level. There are two major probl

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42

Kim, Young-Chan. "Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp)." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3204540.

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Thesis (Ph. D.)--Indiana University, Dept. of Chemistry, 2006.<br>Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0249. Adviser: C. Cheng Kao. "Title from dissertation home page (viewed Feb. 9, 2007)."

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Liang, Yuying. "Molecular characterization of rubella virus nonstructural proteins and viral RNA synthesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0020/NQ56576.pdf.

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44

Firth, Andrew Graeme. "Synthesis and Characterisation of Fluorescent RibonucleotideSubstrates for DNA Dependent RNA Polymerases." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.507614.

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45

Tolbert, Thomas J. (Thomas James) 1969. "Synthesis of RNA with selective isotopic labels for NMR structural studies." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/50341.

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46

Küpfer, Pascal André. "Synthesis, chemical stability and processing by reverse transcriptases of abasic RNA /." [S.l.] : [s.n.], 2006. http://www.zb.unibe.ch/download/eldiss/06kuepfer_pa.pdf.

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47

Kollappillil, Somakumar Krishnakumar. "Synthesis and analysis of puromycin analogues and amphiphilic peptidyl-RNA conjugates." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10086.

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Une étude récente sur le transfert peptidique pH dépendant effectuée avec divers ARNt aminoacyles a révélé la dépendance au pH du transfert peptidique. L’instabilité hydrolytique rend impossible l’obtention de la valeur expérimentale du pKa de l’eau donné pour le groupement α-amino des esters 3'-aminoacyladenosine. Comme les analogues de la puromycine sont les analogues les plus proches du 3’-terminal des ARNt aminoacyles et qu’ils contiennent une liaison amide stable en position 3’, il est intéressant de déterminer la valeur du pKa du groupement α-amino de différents analogues de la puromycin

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48

Dingwall, C. "The accumulation of proteins in the Xenopus oocyte nucleus." Thesis, Open University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354967.

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The ability of proteins to accumulate in the nucleus has been studied by injecting nucleoplasmin and calf thymus histone H1 into the cytoplasm of Xenopus oocytes. Nucleoplasmin, the most abundant protein in the Xenopus oocyte nucleus is pentameric and proteolysis of the nuceloplasmin pentamer produces a relatively protease resistant 'core' molecule that cannot enter the nucleus after microinjection into the cytoplasm. The polypeptide domain ('lq tail') of each subunit removed by proteolysis was obtained as a discrete fragment and has the ability to accumulate in the nucleus. Partially cleaved

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49

Deutsch, Christopher Wayne. "Discovery and Characterization of the Proteins Involved in the Synthesis of N⁶-Threonylcarbamoyl Adenosine, a Nucleoside Modification of tRNA." PDXScholar, 2016. http://pdxscholar.library.pdx.edu/open_access_etds/3080.

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N6-threonylcarbamoyl adenosine (t6A) is a universally conserved tRNA modification found at position 37 of tRNAs which decode ANN codons. Structural studies have implicated its presence as a requirement for the disruption of a U-turn motif in certain tRNAs, leading to the formation of properly structured anticodon stem loop. This structure is proposed to enhance the base pairing between U36 of tRNA and A1 of the codon which aids in translational frame maintenance. Despite significant effort since its discovery in the 1970s the enzymes involved in its biosynthesis remained undiscovered. Bioinfor

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Li, Tin-wai Olive, and 李天慧. "Influenza polymerase subunit compatibility between human H1 and H5 viruses." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41896890.

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