Academic literature on the topic 'RNAseq Unicellulaire'

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Journal articles on the topic "RNAseq Unicellulaire"

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Worden, Alexandra Z., Sallie W. Chisholm, and Brian J. Binder. "In Situ Hybridization of Prochlorococcusand Synechococcus (Marine Cyanobacteria) spp. with rRNA-Targeted Peptide Nucleic Acid Probes." Applied and Environmental Microbiology 66, no. 1 (2000): 284–89. http://dx.doi.org/10.1128/aem.66.1.284-289.2000.

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ABSTRACT A simple method for whole-cell hybridization using fluorescently labeled rRNA-targeted peptide nucleic acid (PNA) probes was developed for use in marine cyanobacterial picoplankton. In contrast to established protocols, this method is capable of detecting rRNA inProchlorococcus, the most abundant unicellular marine cyanobacterium. Because the method avoids the use of alcohol fixation, the chlorophyll content of Prochlorococcus cells is preserved, facilitating the identification of these cells in natural samples. PNA probe-conferred fluorescence was measured flow cytometrically and was
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Liu, Zeye, Hong Jiang, Fengwen Zhang, et al. "Abstract 4140707: The Examination of Expression Patterns, Underlying Mechanisms, Diagnostic Accuracy, and Potential AI-Driven Drug Development Approaches for Ferroptosis-Related Genes in Heart Failure via Single-Cell and Bulk RNA Sequencing Analyses." Circulation 150, Suppl_1 (2024). http://dx.doi.org/10.1161/circ.150.suppl_1.4140707.

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Introduction: Heart failure is a significant concern for public health, particularly among the elderly demographic. In this study, we aimed to investigate the molecular underpinnings of heart failure, focusing on ferroptosis-related genes. Purpose: Our objective was to analyze unicellular RNA sequencing data derived from patients with heart failure and healthy controls. We sought to identify common ferroptosis-related genes and explore their potential as therapeutic targets for heart failure treatment. Methods: We analyzed unicellular RNA sequencing data from 200,615 cells, encompassing sample
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Murakami, Suzuna, Hiroki Takahashi, Kaede Shimizu, and Tomohito Yamasaki. "Global Identification of AGO3–RNA Interactions Reveals Targets of Small RNA–mediated Gene Regulation in Chlamydomonas reinhardtii." Plant And Cell Physiology, April 17, 2025. https://doi.org/10.1093/pcp/pcaf040.

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Abstract MicroRNAs (miRNAs) form complexes with Argonaute (AGO) proteins and bind to mRNAs with complementary sequences to repress their expression. Organisms typically possess several hundred miRNAs that regulate diverse aspects of biology. Although the roles of miRNAs have been elucidated in multicellular organisms, they remain largely unexplored in unicellular organisms. Identifying miRNA target genes remains challenging in the green alga Chlamydomonas (Chlamydomonas reinhardtii), the first unicellular organism in which miRNAs were discovered. Previous computational and sequencing-based app
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McDermott, Suzanne M., Vy Pham, Brian Oliver, Jason Carnes, D. Noah Sather, and Kenneth D. Stuart. "Deep mutational scanning of the RNase III-like domain in Trypanosoma brucei RNA editing protein KREPB4." Frontiers in Cellular and Infection Microbiology 14 (April 8, 2024). http://dx.doi.org/10.3389/fcimb.2024.1381155.

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Kinetoplastid pathogens including Trypanosoma brucei, T. cruzi, and Leishmania species, are early diverged, eukaryotic, unicellular parasites. Functional understanding of many proteins from these pathogens has been hampered by limited sequence homology to proteins from other model organisms. Here we describe the development of a high-throughput deep mutational scanning approach in T. brucei that facilitates rapid and unbiased assessment of the impacts of many possible amino acid substitutions within a protein on cell fitness, as measured by relative cell growth. The approach leverages several
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sprotocols. "The Genus Antirrhinum (Snapdragon): A Flowering Plant Model for Evolution and Development." December 30, 2014. https://doi.org/10.5281/zenodo.13629.

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Authors: Andrew Hudson, Joanna Critchley and Yvette Erasmus Corresponding author ([andrew.hudson@ed.ac.uk](andrew.hudson@ed.ac.uk)) ### INTRODUCTION The *Antirrhinum* species group comprises approximately 20 morphologically diverse members that are able to form fertile hybrids. It includes the cultivated snapdragon *Antirrhinum majus*, which has been used as a model for biochemical and developmental genetics for more than 75 yr. The research infrastructure for *A. majus*, together with the interfertility of the species group, allows *Antirrhinum* to be used to examine the genetic basis for pla
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Dissertations / Theses on the topic "RNAseq Unicellulaire"

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Koshy, Aysis. "Characterization of Neural Development : Linking Retinoic Acid Receptors to Cell Fate and Modelling Tumorigenesis in Brain Organoids." Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPASL119.

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Le développement du système nerveux central dans l'embryon dépend d'une signalisation opportune et précise des molécules. L'acide rétinoïque est l'une de ces molécules bien caractérisées par son impact sur le développement du cerveau et des yeux. Sous sa forme métaboliquement active, l'ATRA (acide All Trans Retinoïque) se lie aux récepteurs de l'acide rétinoïque (RAR) et contrôle l'expression d'une panoplie de gènes participant à des évènements impliqués dans la maturation cellulaire ainsi qu'à l'apoptose. Le RAR existe sous trois isotypes - RARα, RARβ et RARγ. Au cours du développement embryo
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