Academic literature on the topic 'Rock lobster'

ABSTRAK Lobster (Panulirus spp.) adalah komoditas perikanan penting dan ekonomis tinggi. Spesies yang paling sering dan dominan tertangkap adalah lobster batu. Meningkatnya permintaan lobster menjadikan nelayan melakukan kegiatan penangkapan secara terus menerus. Penelitian ini bertujuan untuk menganalisis hubungan panjang-berat dan rasio jenis kelamin (sex ratio) lobster batu (Panulirus penicillatus). Penelitian dilakukan pada Februari 2017 sampai dengan Agustus 2017 di pantai selatan Yogyakarta. Kajian stok lobster dilakukan melalui survei yang meliputi metode deskriptif, observasi dan wawancara. Pemilihan lokasi pengambilan sampel dilakukan secara terpilih (purposive) yaitu pantai Ngrenehan, Baron, Drini dan Tepus. Total sampel lobster batu yang diperoleh sepanjang penelitian sejumlah 546 ekor, yang terdiri dari lobster jantan 289 ekor (53%) dan lobster betina 257 ekor (47%). Hasil penelitian menyatakan bahwa pola hubungan panjang dan berat lobster batu memiliki sifat allometrik negative dengan persamaan W= 0,004 L 2,577 pada lobster jantan dan W = 0,007 CL 2,481 pada lobster betina. Perbandingan nisbah kelamin lobster batu jantan dan betina adalah 1,12 : 1. ABSTRACTLobster (Panulirus spp.) is an important and economically high fishery commodity. The most frequent and dominant species caught is rock lobster. The increasing demand for lobsters makes fishermen conduct continuous catching activities. The study aims to analyze the relationship of length-weight and sex ratio of rock lobster (Panulirus penicillatus). The research was conducted from February 2017 to August 2017 in southern coast of Yogyakarta. The study of lobster stocks was conducted through surveys that included descriptive methods, observations and interviews. The sampling location was chosen purposively, namely Ngrenehan, Baron, Drini and Tepus. Total samples obtained throughout the study amounted to 546 double-spined rock lobster, consisting of 289 males (53%) and 257 females (47%). The results stated that the length and weight relationship pattern of rock lobsters is negative allometric, with the equation W= 0,004 L 2,577in males lobster and W = 0,007 CL 2,481 in females lobster. The sex ratio double-spined rock lobster between males and females lobster is 1,12 : 1.

Abstract Underwater imaging is being extensively used for monitoring the abundance of lobster species and their biodiversity in their local habitats. However, manual assessment of these images requires a huge amount of human effort. In this article, we propose to automate the process of lobster detection using a deep learning technique. A major obstacle in deploying such an automatic framework for the localization of lobsters in diverse environments is the lack of large annotated training datasets. Generating synthetic datasets to train these object detection models has become a popular approach. However, the current synthetic data generation frameworks rely on automatic segmentation of objects of interest, which becomes difficult when the objects have a complex shape, such as lobster. To overcome this limitation, we propose an approach to synthetically generate parts of the lobster. To handle the variability of real-world images, these parts were inserted into a set of diverse background marine images to generate a large synthetic dataset. A state-of-the-art object detector was trained using this synthetic parts dataset and tested on the challenging task of Western rock lobster detection in West Australian seas. To the best of our knowledge, this is the first automatic lobster detection technique for partially visible and occluded lobsters.

Protected areas for the South African rock lobster, Jasus lalandii, were sampled by using divers, traps and ringnets at sites within and adjacent to four protected areas (St Helena Bay, Saldanha Bay and Table Bay rock lobster sanctuaries and the Betty’s Bay marine reserve), over two years. Virtually no rock lobsters were found in St Helena Bay sanctuary, probably because of periodic harmful algal blooms. Abundance was greater in Saldanha Bay sanctuary than in adjacent fished areas, but only once in two years. Sizes were, however, larger in this sanctuary than the fished areas. By an order of magnitude, fewer and smaller rock lobsters were caught within the Table Bay sanctuary than in adjacent areas. Only at Betty’s Bay were rock lobster sizes and abundance consistently greater inside than outside the reserve. Fecundity was similar among sites, with females in protected areas contributing no more to egg production than would be expected by the proportional area occupied by protected areas. Rock lobsters do benefit from protection in Betty’s Bay reserve, but the west coast sanctuaries appear poorly located and seemingly contain large areas of unsuitable substrate. They clearly need relocation to be effective.

The western rock lobster (Panulirus cygnus) fishery is one of the largest rock lobster fisheries in the world with an average catch (1974–75 to 1983–84) of 10 million kg. On average, 18 million undersize rock lobsters (sublegal size) are caught, handled, and returned to the sea each season despite the release of many through mandatory escape gaps in traps. Depending on the handling procedure, undersize rock lobsters experience various degrees of exposure, displacement, and damage which adversely affect their survival and growth. Laboratory, field, and tagging experiments showed that handling produced a mortality of 14.6% that costs the industry in excess of $A13 million per season (1984–85 values). An education programme has resulted in improved handling of undersize rock lobsters, and trials have shown that by increasing the size of the escape gap, the number of undersize rock lobsters handled can be reduced by 40–60%. This would result in a significant enhancement of recruitment to the fishery.

Most lobster fisheries are characterized by high exploitation rates. This has led to substantial declines in the size structures of the populations over time as larger lobsters have been removed. Although both scientists and fishers have suggested that size related hierarchies could impact on lobsters entering traps, the effect of the size change on the selectivity of lobster traps as a population's size structure changes has not been investigated. This paper demonstrates that larger lobsters affect the entrapment of smaller lobsters and that this behaviour affects the selectivity of lobster traps. Both spatial and temporal (within season) factors were found to affect the selectivity plots. Spatial differences in selectivity were attributed to the broader size range of larger lobsters found in regions of faster growth. Temporal differences were attributed to the decline in larger lobsters over the course of a season caused by exploitation. There are also differences in trap selectivity between the sexes.

Nonspecific antibacterial activity (ABA) of lobster haemolymph was investigated as an indicator of lobster immune-system status and health condition. To determine whether ABA was localised in the plasma or in the haemocytes, we destroyed the haemocyte membrane by sonication and analysed samples containing plasma and haemocyte lysates for ABA along with sera from the same lobsters. Haemocytes were the main site of ABA in lobster haemolymph. In an experiment designed to assess typical handling conditions, lobsters were placed for 6 h under treatment conditions simulating five transport/storage approaches: submerged in flow-through water, sprayed with flow-through water, submerged in recirculating water, sprayed with recirculating water, and exposed to humid air. The lowest ABA was observed in the two submerged treatments and the highest in the two spray treatments. Lobsters that subsequently survived a simulated live-shipment procedure exhibited significantly lower ABA than did those found dead or weak during a subsequent 7 days of holding in the tank. We concluded that handling stress is associated with high ABA level in lobster haemolymph.

AbstractThe Western Kangaroo Island Marine Park (WKIMP) was declared as part of South Australia's representative system of Marine Protected Areas in 2009. Sanctuary Zone 3 (SZ-3) of the WKIMP is a no-take area protected from fishing since 1 October 2014 and is located within the Northern Zone Rock Lobster Fishery (NZRLF). In February 2017, a dedicated survey was undertaken to estimate the relative abundance (catch per unit effort (CPUE), kg/potlift) and size of southern rock lobster (Jasus edwardsii) inside and outside SZ-3. Survey results were then compared with historical estimates of abundance and size obtained from commercial fishery-dependent data. Survey estimates of relative abundance of legal-size lobsters were 4.4 times greater inside SZ-3 compared with outside in 2017. Since 2014, when fishing was last permitted inside SZ-3, the relative abundance of lobsters increased by 75%. The mean size of legal-size female and male lobsters also increased by 4.1% and 12.5%, respectively. The population responses recorded are consistent with the results recorded for southern rock lobster stocks in marine parks in other jurisdictions.

Paralytic shellfish toxins (PST) are found in the hepatopancreas of Southern Rock Lobster Jasus edwardsii from the east coast of Tasmania in association with blooms of the toxic dinoflagellate Alexandrium catenella. Tasmania’s rock lobster fishery is one of the state’s most important wild capture fisheries, supporting a significant commercial industry (AUD 97M) and recreational fishing sector. A comprehensive 8 years of field data collected across multiple sites has allowed continued improvements to the risk management program protecting public health and market access for the Tasmanian lobster fishery. High variability was seen in toxin levels between individuals, sites, months, and years. The highest risk sites were those on the central east coast, with July to January identified as the most at-risk months. Relatively high uptake rates were observed (exponential rate of 2% per day), similar to filter-feeding mussels, and meant that lobster accumulated toxins quickly. Similarly, lobsters were relatively fast detoxifiers, losing up to 3% PST per day, following bloom demise. Mussel sentinel lines were effective in indicating a risk of elevated PST in lobster hepatopancreas, with annual baseline monitoring costing approximately 0.06% of the industry value. In addition, it was determined that if the mean hepatopancreas PST levels in five individual lobsters from a site were <0.22 mg STX equiv. kg−1, there is a 97.5% probability that any lobster from that site would be below the bivalve maximum level of 0.8 mg STX equiv. kg−1. The combination of using a sentinel species to identify risk areas and sampling five individual lobsters at a particular site, provides a cost-effective strategy for managing PST risk in the Tasmanian commercial lobster fishery.

Total and differential haemocyte counts (THCs and DHCs) were obtained from 36 white and 36 red western rock lobsters (Panulirus cygnus) that had experienced stress during capture and transportation. The lobsters were divided into three different groups on the basis of their holding time in the factory tanks and their health status (fresh arrivals, healthy and moribund). THCs were obtained with a haemocytometer from fresh haemolymph stained with gentian violet. THCs ranged from 2.5 106 to 15.9 106 cells mL-1 in the three groups, being lowest in the moribund lobsters. DHCs were obtained from haemolymph smears stained with both Giemsa and May–GrÜnwald. Three major haemocyte groups could be identified in lobster haemolymph: hyalinocytes, semi-granulocytes and granulocytes. The proportion of hyalinocytes in the three lobster groups ranged from 29.1% to 37.0%, that of semi- granulocytes from 51.1% to 62.9%, and that of granulocytes from 5.1% to 13.1%. The proportion of granulocytes declined in lobsters after they were held for 16 h in the factory tanks and was lowest in moribund lobsters. The results show that THCs and DHCs may be useful in assessing stress or health status in the western rock lobster or other lobsters.

Ecosystem-based fisheries management (EBFM) requires the expansion of fisheries research programs to include the relationship between target species and their habitats such that trophic and other ecological interactions can be assessed. The western rock lobster (Panulirus cygnus) is an ecologically important species that supports Australia’s most valuable single-species fisheries. We tested the relationship between abundance and size of western rock lobster and benthic habitats based on the annual independent breeding stock survey and benthic towed video transects. The work was undertaken at Dongara, Jurien Bay and Lancelin, Western Australia between 2005 and 2007. Abundance of western rock lobster was significantly but moderately related to benthic habitat (adjR2 = 0.28), with high abundances associated with high cover of mixed assemblage and Ecklonia sp. Size was effectively predicted by habitat (adjR2 = 0.65) with larger lobsters found in mixed assemblages with sponge and smaller lobsters associated with mixed assemblage with Ecklonia sp. Our study has shown that understanding the influence of habitat and fishing pressure on the abundance and size of targeted species is a critical step in the effective implementation of EBFM.

The diversification of current aquaculture practise in South Africa is crucial for the future development of the industry. The rock lobster, Jasus lalandii has been identified as a possible candidate species for shore based culture in systems similar to those used for abalone farming. Reduced Total Allowable Catch (TAC's) and an increased minimum legal size were implemented as management strategies to relieve fishing pressure on natural stocks of J. lalandii, and in turn led to increased market demand and a possible niche for farmed lobsters. High puerulus settlement along the Namibian coast, together with other favourable aquaculture attributes, indicate that the commercial grow out of wild harvested juveniles could be feasible. The aim of study was to investigate the feasibility of growing out rock lobsters in shore based systems in Namibia and South Africa. The experimental objectives of the project were to investigate the nutritional requirements, as well as the effect of stocking density and tank design on growth and survival of J. lalandii. The economic viability of shore based rock lobster farming was then evaluated based on the experimental results and typical capital requirements of an abalone farm in South Africa. Puerulus and early juvenile rock lobsters were collected in Luderitz, Namibia and transported to HIK Abalone Farm in Hermanus, South Africa where they were acclimated in black fibre glass tanks in a flow through system. The pueruli were stocked at 50, 75, 100 and 125/m² and early juveniles at 20, 30, 40 and 50/m² to test the effect of density on growth and survival. A comparative dietary study with both size classes was conducted using a mussel diet (Choromytilus meridionalis and Mytilus galloprovincialis), a commercial shrimp feed diet and a rotational diet comprising both mussels and shrimp feed. Tanks provided with "v-shaped" asbestos hides, PVC hides and no hides were compared to test the effect on growth rate and survival of pueruli. All treatments were conducted in triplicate. Temperatures were recorded twice daily while water quality parameters were tested every second week. The results indicate that density had a significant effect on growth and survival on post-pueruli. An initial stocking density of 75 post-pueruli/m² is regarded as optimal both in terms of the growth rate and biomass production per tank. No differences in growth and survival rates were attained in the early juvenile size class. This indicates that higher densities can be used to rear juvenile J. lalandii. No significant differences were obtained in growth rate when reared under different hide conditions although asbestos hides yielded significantly higher survival rates (93.3 %) compared to the tanks provided with PVC hides (74.04 %) and no hides (77.8 %, p≤0.05). As a result of the high survival rate observed in tanks provided with "V-shaped" asbestos hides, these tanks also yielded higher biomass production per tank (297.8 grams) compared to the tanks provided with PVC hides and no hides (261.09 and 260.5 grams respectively). In the diet trials, growth rates of post pueruli and juvenile lobsters fed the mussel and rotational (mussel and shrimp feed) diets did not differ significantly, however, growth rates of lobsters fed the shrimp feed only diet was significantly lower than both the mussel and rotational diets (p≤0.05). Lobsters fed the mussel diet yielded significantly higher survival rates compared to lobsters fed either the rotational diet or shrimp feed diet only. An imbalance in the fatty acid profile of the shrimp feed could have been a major contributing factor to the poor growth and survival in lobsters fed the shrimp feed only diet as the level of linoleic acid (LOA) was exceptionally high resulting in a high (n -6):(n-3) ratio (1.01) compared to the mussel and rotational diets (0.17 and 0.56 respectively). The low level of arachidonic acid (ARA) in the shrimp feed diet could be a further contributing factor explaning for the poor growth and survival of juvenile rock lobsters. An economic feasibility study was conducted at the end of the experimental phase by modelling a hypothetical shore based rock lobster farm. A projection of production costs and revenues was based on the typical costs of a shore based abalone farm and the current market prices for wild harvested J. lalandii. The economic viability was evaluated using benefit-cost ratios, payback period, internal rate of return and breakeven analysis. Sensitivity analyses revealed that the projected lobster growth and survival rates were the main biological factors influencing the economic feasibility of the hypothetical rock lobster farm. An assumed four year grow out period at a low stocking density yielded more lucrative internal rate of return (IRR), benefit-cost ratio, payback period and net present values (NPV) than a five year grow out period at a high density. The four year grow out scenario proved to be more robust to the fluctuating Rand/US$ exchange rate and could accommodate a lower lobster survival rate. Results presented in this study indicate that rock lobster farming is a marginal commercial prospect based on current production performance and costs. Further research to develop effective puerulus collection techniques as well as to make lobster grow out technology more cost effective is required.

Few studies of rock-lobster diet have included analyses of mature females or juveniles of either sex. This study focused on the diet of male and female West Coast rock lobsters (Jasus lalandii) in three size classes (viz.: 10 - 35 mm CL (carapace length) - small, 40 - 59 mm CL - medium and 70 - 85 mm CL - large), using visual analyses of stomach contents. The principle aims were to examine potential differences in diet between: (1) male and female rock lobsters; (2) changes in diet with size and (3) a comparison of rock-lobster diet between areas of known fast- and slow-growth rates. The primary prey items of rock lobsters are shown to be the black mussel (Choromytilus meridianalis), ribbed mussel (Aulacomya ater), barnacle (Notomegabalanus algicola), sea urchin (Parechinus angulosus), sponge and crustacean remains. There was no difference in diet between male and female rock lobsters within any size class. Bray-Curtis similarity dendograms and Multi-Dimensional scaling plots revealed differences between small, medium and large rock-lobster diets. Small rock lobsters consumed mainly ribbed and black mussels, whereas medium rock lobsters consumed higher percentages of barnacle and sponge. Sea urchins comprised a substantial percentage of large rock-lobster diet. ANOSIM (Primer v 4.0) established significant differences (p < 0.05) among diets of the three size classes. Differences in diet were also observed between areas of fast- and slow-growth rates, but these were limited to the small and medium size classes. Key prey items responsible for this difference in diet were black mussel and rock-lobster remains. The gut fullness index decreased with increasing rock-lobster size. In conclusion, there appears to be no difference in diet between male and female rock lobsters, regardless of their size. However, rock-lobster diet does appear to vary with size. The inverse relationship between gut fullness indices and size, suggests that small rock lobsters, which have a higher moult frequency, feed relatively more frequently than larger rock lobsters. Diet composition plays a role in determining the growth rate of rock lobsters from different areas.

Thesis (PhD)--Stellenbosch University, 2015.<br>ENGLISH ABSTRACT: The West Coast rock lobster (WCRL), Jasus lalandii, is a critical marine fisheries resource for South Africa and may in future be negatively affected by the changes in seawater parameters associated with the ongoing anthropogenic carbon dioxide (CO2) emissions. These CO2 emissions have been linked to a global decrease in ocean pH (termed “ocean acidification”) and an increase in temperature. There are strong estimates that these changes are to worsen in coming centuries. This warranted research because of 1) the low current level of the resource (2.6% of pristine) and 2) the relatively unexplored physiological- and other biological responses of the WCRL to environmental stressors. This information is essential for the sustainable management of the resource by government scientists in times of global- and regional climate change. In the short term, it was found that the WCRL was able to rapidly and reversibly respond to acute changes in seawater pH (pH 7.4), this was achieved primarily through the active up-regulation of bicarbonate levels in the haemolymph. Maintaining extracellular pH protects oxygen transport mechanisms, which are sensitive to pH changes due to the large Bohr effect that this study also revealed, in the respiratory protein, haemocyanin of adult WCRL. The energy cost of actively maintaining extracellular pH, however, is expected to affect growth and potentially survival in the long term. This was tested on juvenile WCRL that were exposed to a reduced seawater pH of 7.3 (18.8 °C) over a period of 28 weeks. Results revealed that survival was not influenced and acid-base regulation in the hypercapnia-exposed lobsters was maintained throughout the duration of the trial, however, this led to a reduced growth rate. Subsequently, in order to replicate field conditions more closely, a combination of effects, namely seawater pCO2 (pH 8 and 7.3) and different temperatures (15.6 and 19 °C) on the growth of juvenile WCRL were assessed over an exposure period of 48 weeks in a second chronic trial. In contrast to the initial trial (28 weeks), where hypercapnia was assessed separately, lobsters exposed to hypercapnia had a higher growth rate than those at the same temperature exposed to a “natural” (normocapnic) seawater pH. The difference was interpreted as an indication that food availability/quality may negatively affect stress response, as feeding in the first trial was later considered “sub-optimal” in comparison to that of the second trial. In the latter, although both hypercapnia and temperature affected growth rates, temperature was the largest contributor to differences observed between treatments. The order of growth rates for lobsters from different treatments was: hypercapnia/high temperature > normocapnia/high temperature > hypercapnia/low temperature > normocapnia/low temperature. In this trial too, irrespective of treatment, lobsters were able to maintain extracellular pH within a relatively narrow range over the extent of the trial and survival was not negatively affected by hypercapnia or high temperature. In order to compare the sensitivity of juvenile WCRL to that of adults, with regards to the effect of changes in extracellular pH on oxygen transport, and to assess the impact of chronic hypercapnia, haemocyanin from juveniles was studied in detail after the first growth trial. This revealed that juvenile WCRL have a similar Bohr effect to that of adults. In addition, the haemocyanin of hypercapnia-exposed juveniles showed an increased affinity to oxygen caused by an intrinsic change in its molecular structure. This was interpreted as an energy-saving mechanism, because at the same time, haemocyanin concentration in these animals was lower than in normocapnic lobsters. At the termination of the second chronic trial, the immunological response to the combined stressors was assessed, namely total circulating haemocyte counts (THC) and the ability to clear/inactivate an introduced dose of a bacterium, Vibrio anguillarum. A pilot experiment on non-treated juveniles revealed a similar resting THC to that of other lobster species, and culturable V. anguillarum was rapidly cleared from their haemolymph. The effect of chronic exposure to a combination of effects, namely hypercapnia and different temperatures, was subsequently tested after termination of the second chronic trial. There were no differences between treatments in a) baseline THC (i.e. before bacterial challenge) and 2) the capability to clear culturable bacteria from haemolymph. The only difference was the circulating THCs post-bacterial challenge, as they were reduced in the hypercapnic-, high temperature treatment, compared with all other treatments. The reason is unknown, but it is speculated that it may have been linked to an increased metabolic demand in these lobsters. Overall, these results demonstrate the great plasticity of the WCRL at the molecular-, biochemical and physiological level. They provide important initial information for government fisheries scientists to aid in predicting future development of, and potential threats to the WCRL resource, as well as providing a platform from which the direction of future studies can be determined.<br>AFRIKAANSE OPSOMMING: Die Weskus-seekreef, Jasus lalandii, is ’n belangrike seevisseryhulpbron vir Suid-Afrika en kan in die toekoms negatief geraak word deur die veranderinge in seewaterparameters wat met voortgesette antropogeniese vrystellings van koolstofdioksied (CO2) verband hou. Hierdie CO2-vrystellings word met ’n wêreldwye daling in die pH van seewater (oftewel “oseaanversuring”) en ’n temperatuurstyging verbind. Alles dui daarop dat hierdie veranderinge in die volgende eeue sal vererger. Dít regverdig navorsing weens 1) die huidige skaarste aan dié hulpbron (2,6% van oorspronklike getalle), en 2) die betreklik onverkende fisiologiese en ander biologiese reaksies van die kreef op omgewingstressors. Hierdie inligting is noodsaaklik om staatswetenskaplikes in staat te stel om die hulpbron te midde van wêreldwye en streeksklimaatsverandering volhoubaar te bestuur. Op kort termyn word daar bevind dat die Weskus-kreef vinnig en omkeerbaar op akute veranderinge in die pH van seewater reageer (pH 7,4). Dít is hoofsaaklik deur die aktiewe opwaartse regulering van bikarbonaatvlakke in die hemolimf vasgestel. Die handhawing van ekstrasellulêre pH beskerm die meganismes wat suurstof vervoer, wat gevoelig is vir pH-veranderinge weens die beduidende Bohr-effek in die respiratoriese proteïen, hemosianien, by die volwasse kreef – nóg ’n bevinding van hierdie studie. Tog sal die energiekoste verbonde aan die handhawing van ekstrasellulêre pH na verwagting groei en moontlik ook oorlewing op lang termyn beïnvloed. Dít is getoets op jong Weskus-krewe wat oor ’n tydperk van 28 weke aan seewater met ’n verlaagde pH van 7,3 (18,8 °C) blootgestel is. Resultate dui daarop dat oorlewing nié geraak word nie, en dat suur-basis-regulering in die hiperkapnie-blootgestelde krewe vir die volle duur van die proef gehandhaaf is, hoewel dit tot ’n verlaagde groeitempo gelei het. Ten einde natuurlike omstandighede akkurater na te boots, is ’n kombinasie van uitwerkings, naamlik pCO2 van seewater (pH 8 en 7,3) en verskillende temperature (15,6 en 19 °C), op die groei van jong krewe oor ’n blootstellingstydperk van 48 weke in ’n tweede chroniese proefneming beoordeel. In teenstelling met die aanvanklike proef (28 weke), is hiperkapnie afsonderlik beoordeel en het krewe wat aan hiperkapnie blootgestel is ’n hoër groeitempo getoon as dié by dieselfde temperatuur wat aan seewater met ’n ‘natuurlike’ (normokapniese) pH blootgestel is. Dié verskil is vertolk as ’n aanwyser dat voedselbeskikbaarheid/-gehalte ’n negatiewe uitwerking op stresreaksie kan hê, aangesien voeding in die eerste proefneming later as ‘suboptimaal’ beskou is vergeleke met dié van die tweede proef. In die tweede proef, hoewel hiperkapnie én temperatuur groeitempo’s beïnvloed het, was temperatuur die grootste bydraer tot die verskille wat tussen behandelings opgemerk is. Die orde van die kreefgroeitempo’s met die verskillende behandelings was: hiperkapnie/hoë temperatuur > normokapnie/hoë temperatuur > hiperkapnie/lae temperatuur > normokapnie/lae temperatuur. In die tweede proef kon die kreef ook, ongeag behandeling, ekstrasellulêre pH vir die volle duur van die proefneming binne ’n betreklik beperkte bestek handhaaf, en het nóg hiperkapnie nóg hoë temperatuur ’n negatiewe invloed op oorlewing gehad. Om die gevoeligheid van jong Weskus-krewe met dié van volwasse krewe te vergelyk wat betref die uitwerking van veranderinge in ekstrasellulêre pH op suurstofvervoer, en om die impak van chroniese hiperkapnie te bepaal, is die hemosianien van jong krewe deeglik ná die eerste groeiproef bestudeer. Dít het aan die lig gebring dat die jong kreef ’n soortgelyke Bohr-effek as volwassenes toon. Daarbenewens toon die hemosianien van hiperkapnie-blootgestelde jong krewe ’n verhoogde affiniteit tot suurstof, wat deur ’n intrinsieke verandering in molekulêre struktuur veroorsaak word. Dít is as ’n energiebesparingsmeganisme vertolk, aangesien hemosianienkonsentrasie by hierdie diere terselfdertyd laer was as by normokapniese kreef. Aan die einde van die tweede chroniese proefneming is die immunologiese reaksie op die gekombineerde stressors beoordeel, naamlik totale sirkulerende hemosiettellings (THC) en die vermoë om ’n toegediende dosis van die bakterie Vibrio anguillarum op te ruim/te deaktiveer. ’n Toetseksperiment met niebehandelde jong krewe dui op ’n soortgelyke rustende THC as dié van ander kreefspesies, en kweekbare V. anguillarum is vinnig uit die hemolimf opgeruim. Die effek van chroniese blootstelling aan ’n kombinasie van faktore, naamlik hiperkapnie en verskillende temperature, is vervolgens na afloop van die tweede chroniese proef getoets. Die verskillende behandelings lewer dieselfde a) THC op die basislyn (met ander woorde voor toediening van die bakterie), en 2) opruimingsvermoë van kweekbare bakterieë uit die hemolimf op. Die enigste verskil was die THC’s ná toediening van die bakterie, wat laer was met die hiperkapniese hoëtemperatuurbehandeling as met alle ander behandelings. Die rede hiervoor is onbekend, maar hou vermoedelik verband met ’n verhoogde metaboliese vereiste by hierdie krewe. Oor die algemeen toon hierdie resultate die beduidende plastisiteit van die Weskus-seekreef op molekulêre, biochemiese en fisiologiese vlak. Dit bied belangrike aanvanklike inligting vir staatsvisserywetenskaplikes om die toekomstige ontwikkeling van én moontlike bedreigings vir die kreefhulpbron te voorspel, en voorsien boonop ’n platform van waar die rigting van toekomstige studies bepaal kan word.

Thesis submitted in fulfilment of the requirements for the degree Master of Technology: Oceanography in the Faculty of Applied Sciences at the Cape Peninsula University of Technology 2014<br>The Tristan lobster Jasus tristani is distributed among several isolated islands and submerged seamounts in the South East Atlantic Ocean. This species occurs only at the Tristan da Cunha group, a British Overseas Territory and the World’s most remote inhabited island, and in international waters at Vema Seamount 1680 km ENE of Tristan. All these populations are exploited commercially. The catch, processing and export of J. tristani is the most important economic activity for the inhabitants of Tristan da Cunha, providing the livelihood of many families and accounting for approximately 80% of the Island’s revenue. Sustainable harvesting of this valuable resource requires accurate long-term data on catch and effort, as well as information on the most important biological parameters such as growth, recruitment, moulting and reproductive cycles. This information is critical for robust assessments and management strategies. This thesis describes the history of the lobster fishery at the Tristan da Cunha island group, reporting on catches and trends in Catch Per Unit Effort between 1967 and 2010. A total of 247,014 lobster samples, both sexes combined, was sampled for size composition and sex ratios, as well as 1,526 lobsters for length/weight relationships, between 1997 and 2010. This confirms earlier findings that females have broader and heavier tails than males for the same carapace length (CL). Results show that males dominate catches at all islands, and their average size was larger than that of females (83.5 ± 14.46 versus 73.4 ± 8.64 mm CL, respectively). Inter-island differences in lobster population structure appear to be caused by differences of food availability as well as in density-dependent growth and survival of young lobsters. The largest lobsters were found at Gough Island (87.2 ± 15.13 mm CL), and the smallest at Inaccessible island (73.2 ± 11.39 mm). Tristan was the next largest to Gough Island (84.0 ± 12.56 mm) followed by Nightingale island (78.2 ± 11.33 mm). Lobsters caught inshore were larger than those caught offshore, although this may be related in part to differences in catches between fishing gear types. This study showed that fecundity increases in a linear manner with CL, and although larger lobsters clearly produce more eggs than smaller ones, the gain in fecundity is not as great as in some lobster species where fecundity is more closely associated with weight. The study showed no significant differences in egg size between islands, or between large and small females at one island. The egg production per gram of body weight and mean egg diameter both seem to be less than reported in an earlier study in the 1990s. While it seems likely that this is due to differences in the way in which samples were collected (with only stage 2 ova collected and measured in this study), the possibility of a decline in fecundity needs to be investigated further. A range of management measures have been developed over the history of the fishery, and important current measures include an annual total allowable catch (TAC) for each island, minimum size limits, and a closed season timed to protect egg-bearing females. The fishery has recently been awarded certification by the Marine Stewardship Council. The study has confirmed that current conversion factors are broadly correct and that different size limits established for each island are justified. Concern is raised, however, by the fall in catch per unit effort and the mean size of lobsters at the three northern islands over the past 7 years. These trends will need to be closely monitored. There are still many uncertainties over key parameters such as growth and recruitment and the intention is to increase the knowledge base and our understanding of the dynamics of the lobster stock. A research plan has been developed, so that progress can be monitored through the gradual implementation of scientifically defendable fisheries management procedures and increased research and monitoring capacity.

An eastward shift in the West Coast rock lobster Jasus lalandii took place in the early 1990s along the south-west coast of South Africa in an area known as East of Cape Hangklip (EOCH). Given the predatory capabilities of J. lalandii, an intricate relationship between the urchin Parechinus angulosus and juvenile abalone Haliotis midae, and an already over-exploited ecosystem, the lobster âinvasionâ is assumed to have had serious consequences on the benthic community and associated fisheries. To investigate these effects, I used both empirical and modelling approaches. Based on field studies, I first analysed temporal changes in rock lobsters and the benthic community at two lobster-invaded sites EOCH. Prior to 1990, rock lobsters were rare EOCH, but from the mid 1990s onwards they achieved densities of 0.4- 0.8 m-2. The pre-invaded benthic community was significantly different from the postinvaded community at both sites. Three major changes followed the lobster invasion: (1) a decline or even a disappearance of herbivores (a direct effect), (2) macroalgal proliferation (an indirect effect) and (3) increases of a range of sessile taxa (unknown effect). This was followed by a detailed spatial analysis of J. lalandii densities and the benthic community EOCH, in which I surveyed six sites (three invaded and three noninvaded) at three different depth zones (< 5 m, 6-12 m and 13-20 m). At all three depth zones Jasus lalandii was significantly more abundant in invaded areas than in noninvaded areas, and invaded and non-invaded benthic communities were significantly different. Invaded sites were characterized by higher densities of rock lobsters, macroalgae and sessile species, whereas non-invaded sites had greater amounts of herbivores and encrusting corallines. Abalone abundance reflected their previouslyrecorded dependency on urchins and the effects of rampant poaching. Floral species diversity was on average greater at invaded sites and increased with depth, whereas faunal species diversity was greater at non-invaded sites but also increased with depth. The depths in which strongest effects of J. lalandii were felt coincided with the depth of maximum abundance of the urchin Parechinus angulosus, the abalone Haliotis midae, the kelp Ecklonia maxima and encrusting corallines. In line with an ecosystem approach to fisheries management and to better understand the ecosystem dynamics EOCH, a lobster-urchin-abalone Minimally Realistic Model was developed for a lobster-invaded area, and an adjacent nonvi invaded area. A key feature of the model is that its focus was restricted to the critical interactions of interest and was fitted to all available data. An existing abalone stockassessment model formed the foundation of this multispecies model, to which rock lobsters and urchins were added. Abalone and rock lobsters were modelled using agestructured production models and urchins were modelled using a simpler surplus production model because of data limitations. The model estimated a lobster starting biomass (in 1985) of 314 tonnes (MT) and a carrying capacity of 1511 MT. Speciesinteraction parameters, particularly the lobster-abalone interaction, were difficult to estimate and the model was unable to estimate the urchin intrinsic growth rate parameter because the data had insufficient contrast. Results from the model suggest that the lobster invasion was probably caused by an influx of adult lobsters as opposed to increased larval settlement. Future projections suggest that given the virtual disappearance of urchins, complete removal of rock lobsters would be needed to allow the urchin population to re-establish itself. Recovery of urchins could take up to 50 years and recovery of abalone would take even longer. The model-predicted differences in lobsters, abalone and urchins between invaded and non-invaded areas paralleled empirical data. Further model explorations included (1) a hypothetical lobster invasion into a currently non-invaded zone EOCH and (2) the addition of a top fish predator into a lobster-invaded zone. Various hypothetical lobster invasions into the non-invaded zone all resulted in the eventual disappearance of urchins and, consequently, juvenile abalone. Available catch and effort data for fish indicated that a major decline in linefish has taken place, but that this occurred prior to the 1980s and was thus not the direct cause of the rock-lobster invasion. This was supported by outputs of a model incorporating fish predation, which demonstrated that the presently over-exploited fish biomass has very little effect on the rock lobster population, but that at historical pristine levels, fish would have been capable of preventing the establishment of a dense rock-lobster population and the consequent disappearance of urchins and abalone. These results indicate that the over-fishing of top-predators would have had massive ramifications for the rest of the ecosystem. Through dual empirical and modelling approaches, my study highlights the complexity of ecosystem interactions and the need for multispecies models in developing an ecosystem approach to fisheries management, and adds to the understanding of the causes and implications of human- and environmentally-induced shifts in community structure.

Off the west coast of Australia between approximately North-West Cape (21°S) and Cape Leeuwin (35°S), from the shoreline to the edge of the continental shelf wherever suitable reef exists, the western rock lobster Panulirus cygnus occurs in prodigious numbers. Although this resource was apparently untapped by the aboriginal Australians of the area its presence became known, at least in part, to early European settlers and efforts were made to harvest some as an adjunct to scale-fisheries for the small local market. More concerted efforts were made near the tum of the century as appropriate catching technology was introduced and local population grew. The potential for a major industry was soon recognised, but efforts to establish processing factories and export markets for a canned or frozen product did not prosper until World War 2 changed the course of events. Since then an intensive fishery has evolved that, as well as being Australia's most valuable single-species fishery, has for several decades been widely recognised as one of the most productive, well-managed and lucrative in the world. Despite its apparent consistency it is anything but static, barely a year passing without significant new developments. This thesis presents a maritime history of the western rock lobster fishery. It provides the first fully researched narrative account of the fishery, chronicling effort, catch and efficiency, demand and marketing, economic conditions, research and management, the life of the fisher at different periods and related social and cultural developments and interactions. Key determinants of the nature and course of this uniquely successful fishery are investigated, including biological, environmental and geographical factors, fishing knowledge, technology, skill and strategy, processing, marketing and economic opportunities and trends, scientific research and management, social, cultural and political influences, innovation, entrepreneurship and the less tangible1 element of luck. This thesis argues that the western rock lobster fishery, until now largely neglected in historical treatises, is deserving of a place in the mainstream history of ll1 Western Australia. It further contends that a multi-disciplinary approach is essential to understanding the nature and course of the fishery and its wider historical context. This study is based on thorough research of official records, contemporary accounts and reports, interviews with fishers and others in the industry from past and present, and first-hand experience gained on vessels fishing for the lucrative, and delectable, western rock lobster.

In a context of declining capture fisheries and public pressure for greater access to marine resources, marine aquaculture is receiving increasing interest from the South African government as a means to increase the diversity of economic activities in coastal regions, thereby providing employment and reducing poverty. The east coast rock lobster Panulirus homarus rubellus is currently harvested by subsistence fisherman along the former Transkei coastline of south-east South Africa and presents a possible opportunity for ongrowing wild juvenile lobsters in culture facilities. Lack of compliance coupled with poor enforcement of the minimum size limit (65 mm carapace length) has resulted in the ongoing harvest of undersize size lobsters by subsistence fishers. Generally, fishers either consume these undersize lobsters or sell them to tourists for low prices. In line with international trends in rock lobster aquaculture, interest has subsequently arisen in the possibilities of ongrowing these undersize lobsters as a means of adding value to the P. h rubellus resource for subsistence fishers. The aim of this physiological study was to assess the biological feasibility of harvesting, transporting and culturing wild caught juvenile lobsters, thereby provide empirical data to inform the development of suitable transport and culture protocols. The experimental objectives were to asses the effect of temperature on growth and survival of P. h. rubellus, as well as the effects of a suite of extrinsic and intrinsic factors on ammonia excretion and oxygen consumption. Juvenile lobsters were collected by hand from near-shore reefs (2-15 m depth) off Mdumbi in the former Transkei, Eastern Cape Province and transported by road (7 hours) to the Port Alfred Marine Research Laboratory where they were held in a recirculating culture system. The effect of temperature over a range of 9.7 °C (18.9±0.7 to 28.6±1.5 °C) on the growth and survival of juvenile P. h. rubellus fed a diet of fresh mussel flesh was investigated. Specific growth rate (SGR) was significantly different between temperatures (p = 0.01), with the highest values recorded for the 24 °C and 28 °C treatments. There was no significant difference in moult increment (MI) between temperatures, however, intermoult period (IMP) differed significantly between temperatures (p = 0.0015) with mean IMP lowest at 24 °C, although not significantly different from the means of the 26 °C and 28 °C treatments. IMP was highest at 19 °C and 21 °C. Apparent feed intake was significantly different between treatments (p < 0.0001) and exhibited a strong positive correlation with increasing temperature. Food conversion ratio (FCR) differed significantly between temperatures (p = 0.02) with 24 °C exhibiting the most efficient FCR. The results for growth rate and food conversion efficiency suggested that 24 °C is optimal for the growout of juvenile P. h. rubellus. In the second study, the effect of body weight, emersion, daily rhythm, feeding and ambient ammonia on the total ammonia nitrogen (TAN) excretion rate was investigated. Body weight (n = 16, range of 16.8 – 322 g) was positively correlated to daytime TAN excretion rate (mg h⁻¹). Re-immersion after one hour emersion in a moist environment was characterized by a significant increase in TAN excretion rate for the first hour compared to pre-immersion levels. The amount of TAN excreted during this period was as expected if basal TAN excretion rates were maintained during emersion. TAN excretion rates returned to pre-emersion levels by the end of the second hour. There was no evidence of a daily rhythm in TAN excretion rate for P. h. rubellus. TAN excretion rates were elevated following feeding. An initial peak in TAN excretion rate after seven hours (7.58 times pre-feeding rate) was followed by a smaller peak after 13 hours (6.69 times pre-feeding rate). TAN excretion rate dropped to levels not significantly different from pre-feeding levels after 23 hours and consistently returned to pre-feeding levels after 42 hours. The TAN excretion rates of lobster exposed for two hours to an ambient TAN concentration of 1.02±0.10 mg l⁻¹ and 2.3± 0.2 mg l⁻¹ were not significantly different from TAN excretion rates recorded at low ambient water TAN prior to exposure. Exposure to an ambient TAN concentration of 4.45±0.78 mg l⁻¹ had a significant effect on the TAN excretion rate, with pronounced ammonia uptake occurring for all animals at this concentration. The third study investigated the effects of body weight, diurnal rhythm, feeding and emersion on lobster oxygen consumption. Body weight was positively correlated to both standard and active oxygen consumption (mg O2 h⁻¹) while body weight was negatively correlated to mass-specific standard oxygen uptake (mg O2 g⁻¹ h⁻¹). Diurnal rhythm exhibited a strong effect on the lobsters oxygen consumption, with average night time values 67% greater than those recorded during the day. This was related to activity driven by intrinsic nocturnal foraging behaviour. Feeding resulted in a classic specific dynamic action (SDA) response, with postprandial oxygen consumption increasing to a peak before decreasing gradually to preprandial levels. Emersion resulted in a significant increase in oxygen consumption, with lobsters rapidly recovering to pre-emersion levels after four hours. Results from these studies suggest that the capture, transport and culture of juvenile P. h. rubellus is biologically feasible. Empirical data generated were used to provide recommendations regarding species optimised transport and culture protocols. A purge time of 48 hours before transport is suggested to ensure that ammonia excretion and oxygen consumption are at basal levels. Furthermore, emersed transport for a period of one hour is characterised by rapid recovery upon re-immersion. In order to prevent the accumulation of stressors, it is suggested that consecutive periods of emersion are interjected with recovery periods (five hours) in water to allow the removal of accumulated ammonia and repayment of the oxygen debt incurred. The recorded ammonia rates indicate that a biological filter size of 4.8 m³ is recommended for 1000 kg of fed lobsters in a culture situation, although this can be reduced considerably if lobsters are being held without feeding (0.72 m³). A flow rate of 112 l kg⁻¹ h⁻¹ is required to meet the metabolic requirements of lobsters. Bottlenecks to the viable commercial culture of P. h. rubellus, and the ability of this practice to provide the socio-economic benefits that were envisioned, are discussed.

A global decline in the lobster fishery has necessitated the growout of wild-caught postlarvae (pueruli) on farms to meet the worlds lobster demand. A limited knowledge on puerulus settlement in the West Coast rock lobster (Jasus lalandii) constitutes a major barrier for J. lalandii culture development in Southern Africa. As an extension of an existing long-term puerulus monitoring program of the Ministry of Fisheries and Marine Resources of Namibia, the present study monitored puerulus recruitment (supply), puerulus condition and juvenile growth of J. lalandii and their relationship with environmental variables on an oyster farm at Lüderitz Bay, Namibia. Implications for lobster aquaculture and fisheries management are discussed. Oyster bags, crates and collectors were sampled daily, weekly and bimonthly, for pueruli and juveniles, respectively, between August 2001 and July 2003 to establish spatialtemporal patterns in their distribution. Animals were transported to the laboratory for morphometric analyses where they were staged as puerulus (Pt = transparent; Pp = pigmented) and juvenile lobsters. The carapace lengths (0.1mm CL) and wet weights (0.01gram) of animals were recorded. Carapace length frequencies were subjected to Modal Progression analysis to identify early juvenile cohorts and calculate their growths. The weight-length ratios calculated and percentage fat content were used as indices of nutritional condition. Seasonal peaks in puerulus and juvenile abundance were estimated using Periodic Regression analysis. The relationships of puerulus and juvenile abundance with respect to sea surface temperature (SST), wind speed-direction, moon phase, swell and tidal range were established using time lag analysis (ADL). Transparent pueruli were randomly distributed on oyster bags and crates, and also between collector sites, suggesting that they have no particular settlement preference for certain areas on these structures (p>0.05; n=150). Pigmented puerulus (p<0.05; n=566) and juveniles (p<0.05; n=3528) preferred structurally complex structures that provided better shelter such as crates, which were more sheltered from sea swells, compared to oyster bags. Among oyster rafts, pigmented puerulus and juveniles preferred occupying the sheltered and centred raft areas, which provided better protection from wave action, compared to exposed raft areas, which were exposed to the incoming swell. Puerulus and juvenile lobster appeared to recolonise adjacent bags and crates, and possibly migrate to the substrate and off the farm due to the disturbance of the habitat during harvesting. The booth crevice collector was more efficient in collecting puerulus possibly due to the high structurally complex habitat and the better shelter they provided compared to the hogshair collector. The occupation of structurally complex habitats by early benthic stages may be related to morphological changes associated with metamorphosis. Puerulus settlement peaked during early spring (August-September) and was related to low sea surface temperatures (12.3°C), strong south winds, and moderate swells in the week preceding settlement. These conditions are synonymous with the “spring upwelling” season of the Lüderitz upwelling cell in the central Benguela current region. Puerulus nutritional condition peaked during this period possibly due to high levels of planktonic food (due to high nutrients from upwelling) that was available to the late stage phyllosoma larvae further offshore. A “good” puerulus settlement year during 2000 was associated with lower than average SST (11.6°C) and strong south winds and was followed by “poor” puerulus settlement years during 2001 and 2002 when higher than average SST were experienced with below average wind speeds experienced during 2001 only. Puerulus of J. lalandii recruited in high numbers into Lüderitz Bay during the new moon, full moon and last quarter moon phases by making use of the increased tides during these phases of the lunar cycle. Peak juvenile abundance during early summer (November-December) lagged by 8 weeks was significantly correlated with peak puerulus abundance. Puerulus size was similar (8.5–9.7mm CL) over the study period. Juvenile cohorts, 9.9-20.6mm CL in size recruited in 2 distinct waves in August 2001-June 2002 and July 2002-February 2003. The 2001/2002 recruits had higher monthly growth increments and better nutritional condition compared to 2002/2003 recruits. Peak frequencies of 2nd moult juveniles (9.9- 10.8mm CL) occurred in September of both recruitment events with nutritional condition being poorest during the 2002/2003 event. The nutritional condition of the settling puerulus did not appear to be related to the subsequent growth of juvenile lobster. These preliminary results suggest that pueruli recruit once a year mainly during late winter/ early spring under conditions typical of coastal upwelling in the Benguela current near Lüderitz. It appears that puerulus settlement is driven by favourable shoreward currents, which help in transporting puerulus to their settlement grounds. Puerulus supply is highly variable between years, which appears to be consistent with a highly variable Benguela environment. The oyster farm supported a substantial standing stock averaging 123 800 lobster per day (range: 22 600-325 300 lobster per day) of which 26 600 were puerulus and 97 200 were juveniles, that may have been harvested once off on any given day during the sample period. Monthly harvesting of the total lobster standing stock during the one and a half year sampling period was just over 2 million lobsters (505 700 pueruli and 1.85 million juveniles) and would potentially have been sufficient seed for a 200 ton lobster production in puerulus lobster growout facilities. In the light of high inter-annual variation in puerulus settlement observed at Lüderitz, the seed supply at the Lüderitz oyster farm is clearly limited and therefore it is prudent that other sites of high puerulus settlement is established before large commercial scale lobster growout can commence. The migration of lobster off the farm suggested that these lobsters potentially recruit to the fishery and therefore harvesting the entire standing stock on the farm may have adverse negative effects on the wild fishery. A policy decision will be required as to whether more efficient harvesting of lobster from the farm should be allowed. If puerulus removal in J. lalandii for aquaculture is allowed in the near future, it should be conducted on an experimental scale or small commercial scale until the potential effects on recruitment into the fishery are clearer.

The condition at settlement of the western rock lobster pueruli presumably reflects both their energetic condition in reaching the near shore and subsequent recruitment into adult populations. In recruiting to the near shore the pueruli swim across the continental shelf where oceanographic conditions are complex and likely influence the success of recruitment. The results from the biochemical studies are interpreted in light of the oceanography off the coast of Western Australia. I investigated the nutritional condition of larval phyllosomata, post-larval puerulus and first instar post-pueruli juveniles of the spiny lobster, Panulirus cygnus, to determine energy use during the non-feeding transitional puerulus stage. Biochemical analyses of lipid, fatty acid (FA) and protein revealed that lipid, in particular phospholipids, is primarily used for energy during the nonfeeding puerulus stage. Monounsaturated FA showed the greatest decline with development, whereas the polyunsaturated FA showed a high degree of sparing, suggesting these FA are not used as a substrate for energy production. The knowledge gained on the biochemistry of energy use in P. cygnus was then used to investigate the spatial and temporal variability in the nutritional condition, in particular lipid condition, of puerulus collected at three near shore locations (Alkimos, Jurien Bay and Dongara) along the Western Australia coast, and one offshore location (Houtman Abrolhos Islands). The one offshore location was chosen as I hypothesised that arriving pueruli are likely to be in a better state of nutrition than those arriving at more coastal locations where the potential journey from offshore larval feeding grounds to the near shore is considerably greater. This element of my research showed lipid levels to be inversely related, generally, to shelf width but were variable, suggesting pueruli may travel complex trajectories to reach nearshore settlement. The lipid and FA composition of pueruli was also consistent with spatial and seasonal variation in Leeuwin Current and coastal productivity regimes.

Lance van Sittert explores the historical records concerning the exploitation of marine resources along the West Coast of Africa, using them to provide evidence of the commerical importance of the West Coast over the East or South. The date demonstrates a boom-bust cycle along the West Coast, pertaining to whales, snoek, guano, rock lobster, hake, and pilchard.

&lt;em&gt;Abstract.&lt;/em&gt; —The American lobster &lt;em&gt;Homarus americanus &lt;/em&gt; is usually associated with rocky substrate that provides or can be modified into shelter and that may be an essential habitat to early benthic-phase juveniles. The dependence on shelter-providing habitat not only makes possible the definition of essential habitat for lobsters but also permits the assessment of abundance based on the areal extent of habitat. Here, we describe such a habitat-based assessment, performed in response to an oil spill on the coast of Rhode Island, USA. Results from a side-scan sonar survey performed after the spill indicated that the amount of lobster habitat affected by the oil was approximately 9.8 km2 along nearly 15 km of coastline. Postspill lobster density ranged from 0.24 lobsters m22 in the impact region to 1.63 lobsters m22 in the control region. Qualitative (map contours of lobster density) and quantitative (statistical tests) approaches suggested a significant effect of the spill had been detected by our sampling. An estimate of the total number of lobsters killed was required to scale restoration efforts. We calculated the total number of lobsters in the area by overlaying contours of lobster density on a habitat map generated by side-scan sonar, then multiplying the density of lobsters in each contour interval by the area of appropriate lobster habitat (cobble and boulder) in the contour interval. To calculate loss, we subtracted postspill abundance from prespill abundance. Prespill density was estimated to be 1.76 m22, which is an adjusted average of airlift samples taken at six Rhode Island sites four months prior to the spill. Calculations of loss based on habitat-specific density estimates were adjusted to reflect undersampling. The loss was estimated to be to be 9.0 × 106 lobsters. Variability associated with this loss estimate is large; 95% confidence intervals estimated that between 6.7 × 106 and 15.6 × 106 lobsters were lost. The calculated loss was very sensitive to changes in prespill density estimates; a change of 0.1 lobsters m22 resulted in a change of 0.75–0.9 × 106 lobsters lost. Habitatbased assessment of lobster population size is possible but requires detailed habitat maps and accurate density estimates. Natural variability and sampling limitations give such assessment a wide range of possible values. Nevertheless, the airlift sampling technique, together with sidescan sonar maps of habitat, could provide a powerful tool for estimating the abundance of inshore lobsters.

We present ideas on using bio-inspired locomotion concepts for robotic underwater applications. The technology of land-based bio-inspired walking robots improved a lot in the last decade; systems like The SCORPION [1], Rhex or DANTE II have proven that high mobility in rough terrain can be achieved. Walking is a locomotion technique which can be found in underwater animals like lobsters, crayfish, or prawn. These animals can be found in the littoral zone of the oceans and rivers, which are a very challenging environment. Crayfish and prawn can live in rough terrain like the slope of a black smoker, too. They are especially able to adapt to irregular ground contours, current, wave action and surge. For example, lobsters can navigate around obstacles such as rocks, crevices and seaweed. Lobsters flourish in the benthic and littoral environments and have developed robust control systems for locomotion, sensing, and searching behavior. These control systems present a proven solution [2, 5, 11]. No artificial wheeled or swimming system is able to navigate as robustly as lobsters or crayfish in such underwater environments. Thus studying the behavior of animals in the littoral zone and using their control principles for robots seems to be a promising way to close this gap. Therefore we analysed past research in this direction, e.g. the ARIEL and the NEU Lobster project and propose new ideas based on our existing systems [1, 3, 15] for a robust underwater walking platform. We present a possible control concept for such an underwater walking system. Furthermore we discuss possible application scenarios like mine detection or environmental monitoring and non-littoral applications like inspection and maintenance of underwater structures and buildings or service for offshore wind energy parks.

Grassroots arts connected to economy through start-up culture Geraldton is a regional centre in Western Australia, with 39,000 people and a stable, diverse economy that includes a working port, mining services, agriculture, and the rock-lobster fishing industry (see Appendix). Tourism, though small, is growing rapidly. The arts and culture ecosystem of Geraldton is notable for three characteristics: - a strong publicly-funded arts and cultural strategy, with clear rationales that integrate social, cultural, and economic objectives - a longstanding, extensive ecosystem of pro-am and volunteer arts and cultural workers - strong local understanding of arts entrepreneurship, innovative business models for artists, and integrated connection with other small businesses and incubators