Academic literature on the topic 'Rocking bioreactor'

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Journal articles on the topic "Rocking bioreactor"

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Ramirez-Malule, Howard, Víctor A. López-Agudelo, David Gómez-Ríos, et al. "TCA Cycle and Its Relationship with Clavulanic Acid Production: A Further Interpretation by Using a Reduced Genome-Scale Metabolic Model of Streptomyces clavuligerus." Bioengineering 8, no. 8 (2021): 103. http://dx.doi.org/10.3390/bioengineering8080103.

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Streptomyces clavuligerus (S. clavuligerus) has been widely studied for its ability to produce clavulanic acid (CA), a potent inhibitor of β-lactamase enzymes. In this study, S. clavuligerus cultivated in 2D rocking bioreactor in fed-batch operation produced CA at comparable rates to those observed in stirred tank bioreactors. A reduced model of S. clavuligerus metabolism was constructed by using a bottom-up approach and validated using experimental data. The reduced model was implemented for in silico studies of the metabolic scenarios arisen during the cultivations. Constraint-based analysis confirmed the interrelations between succinate, oxaloacetate, malate, pyruvate, and acetate accumulations at high CA synthesis rates in submerged cultures of S. clavuligerus. Further analysis using shadow prices provided a first view of the metabolites positive and negatively associated with the scenarios of low and high CA production.
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Vera, Juan F., Lara Brenner, Ann M. Leen, et al. "Rapid Generation of Antigen-Specific T Cells for Pre-Clinical and Clinical Applications Using a Novel Mini Cell Bioreactor." Blood 112, no. 11 (2008): 208. http://dx.doi.org/10.1182/blood.v112.11.208.208.

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Abstract Although flasks, bags, or rocking bioreactors can readily expand T lymphocytes after non-specific stimulation, the requirements for antigen-driven expansion of cytotoxic T lymphocytes (CTLs) are more rigorous. Antigen-specific T cells proliferate optimally only in the 2 mL wells of 24-well plates and cannot reproducibly be adapted to growth in flasks or bags. Hence, preparation of antigen-specific T cells for adoptive immunotherapy of malignancies is extremely time-consuming, requiring between 4wks and 3mths to produce sufficient cells for therapeutic purposes, and expensive (media + plastics + cytokines + man hours). The extensive manipulation required during the culturing process increases the risk of contamination. In combination, these problems obstruct the broader clinical application of antigen-specific T cells. Antigen-specific T cell growth is limited by gas exchange, nutrients and waste buildup. Bioreactors developed to provide these requirements tend to be complex, involving mechanical rocking or stirring and continuous perfusion, which increases the expense of the procedure and limits the number of products to the number of mechanical devices that can be housed and maintained. We have now explored the use of a new static mini Cell Bioreactor for antigen-specific T cell expansion. This device is essentially a flask with a gas permeable membrane supported by a plastic lattice as its base. The O2/CO2 exchange from the base allows large volumes of media to be added thereby reducing nutrient limitations and waste build-up, and consequently the manipulation required to sustain cell expansion. We tested two different sizes of Cell Bioreactor, 10 cm2 and 100 cm2 that hold a maximum of 40mL and 2000mL of media, respectively. We were able to generate and expand Epstein-Barr virus antigen-specific cytotoxic T lymphocytes (EBVCTLs) from normal donors by coculturing antigen presenting cells (APC) (1.4E+05 × cm2) with established EBV-CTL (4.3E+03 × cm2) at an optimized cell density and stimulator: responder ratio (32:1). These culture conditions induced accelerated CTL expansion (42.5 fold ±14.8 vs 3.4 fold ±1.2 within 7 days) without media change. Manipulation was restricted to cytokine addition every 3–4 days and to LCL stimulation on a weekly basis. A single 100cm2 bioreactor could produce up to 800E+06 antigen-specific T cells, which would have required approximately 320 wells in 24 well plates (>13 plates) under standard culture conditions. The CD4:CD8 T cell ratio and phenotype of the Cell Bioreactor-expanded CTLs was similar to those expanded using the conventional method (CD27 48% vs 52.4%, CD28 65.2% vs 62.2%, CD62L 53.15% vs 54.5%, CD45RO 58.1% vs 55.7%, and CD45RA 51.1% vs 54.9%). Antigen specificity, as evaluated by tetramer analysis and IFN-g ELIspot assay demonstrated no significant differences between CTL expanded by each process. Finally, cytolytic function was confirmed using a standard chromium release assay where both sources of CTL had high specific killing of the autologous EBV-transformed LCL targets (85%±12% vs 77%±19%) and minimal killing of allogeneic targets (22%±9% vs 15%±12). In summary, we have successfully utilized the new mini Cell Bioreactor technology to induce optimal in vitro antigen-specific T cell expansion with minimal handling. Future work will evaluate the impact of the accelerated expansion on differentiation and memory markers. This new system is suited to the clinical grade expansion of other cell types including suspension cell lines, and mitogen-activated T cells, as well as T cell blasts engrafted with chimeric antigen receptors.
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Bai, Yun, Murray Moo‐Young, and William A. Anderson. "A mechanistic model for gas–liquid mass transfer prediction in a rocking disposable bioreactor." Biotechnology and Bioengineering 116, no. 8 (2019): 1986–98. http://dx.doi.org/10.1002/bit.27000.

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Davis, Brian M., Evelina R. Loghin, Kenneth R. Conway, and Xiaohua Zhang. "Automated Closed-System Expansion of Pluripotent Stem Cell Aggregates in a Rocking-Motion Bioreactor." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 4 (2018): 364–73. http://dx.doi.org/10.1177/2472630318760745.

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Sauvage, V., M. Janas, S. Stone, A. Marenghi, S. Stubbs, and B. Mark. "Optimization of the rocking speed and angle of a perfusion bioreactor for T cell culture." Cytotherapy 16, no. 4 (2014): S35. http://dx.doi.org/10.1016/j.jcyt.2014.01.117.

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Westbrook, Adam, Jeno Scharer, Murray Moo-Young, Nico Oosterhuis, and C. Perry Chou. "Application of a two-dimensional disposable rocking bioreactor to bacterial cultivation for recombinant protein production." Biochemical Engineering Journal 88 (July 2014): 154–61. http://dx.doi.org/10.1016/j.bej.2014.04.011.

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Bai, Yun, Murray Moo-Young, and William A. Anderson. "Characterization of power input and its impact on mass transfer in a rocking disposable bioreactor." Chemical Engineering Science 209 (December 2019): 115183. http://dx.doi.org/10.1016/j.ces.2019.115183.

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Gratch, Y. S., P. Bowles, R. Krishnan, S. Loo-Yong-Kee, R. K. Iyer, and A. Dulgar-Tulloch. "A semi-automated, high-purity process for natural killer (NK) cell manufacturing in a rocking bioreactor." Cytotherapy 20, no. 5 (2018): e8-e9. http://dx.doi.org/10.1016/j.jcyt.2018.03.029.

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Glazyrina, Julia, Eva-Maria Materne, Thomas Dreher, et al. "High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor." Microbial Cell Factories 9, no. 1 (2010): 42. http://dx.doi.org/10.1186/1475-2859-9-42.

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Cirés, Samuel, Carlos Alvarez-Roa, and Kirsten Heimann. "First use of the WAVE™ disposable rocking bioreactor for enhanced bioproduct synthesis by N2-fixing cyanobacteria." Biotechnology and Bioengineering 112, no. 3 (2014): 621–26. http://dx.doi.org/10.1002/bit.25455.

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Dissertations / Theses on the topic "Rocking bioreactor"

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Westbrook, Adam. "Disposable rocking bioreactors for recombinant protein production in Escherichia coli: Physical characterization and assessment of therapeutic protein expression." Thesis, 2013. http://hdl.handle.net/10012/7927.

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Disposable technology has gained increasing acceptance in the biopharmaceutical industry over the last decade, and provides many advantages over conventional stainless steel equipment. Disposable rocking bioreactors (RBs) are widely employed for cultivation of recombinant mammalian and insect cell lines, although the perception of inadequate mass transfer has prevented their application to bioprocesses based on microbial platforms. In an effort to thoroughly evaluate the suitability of disposable RBs for cultivation of aerobic microorganisms, a comparative study of one-dimensional (1D) and two-dimensional (2D) disposable RBs, and the conventional stirred tank reactor (STR) was performed. The comparison involved: 1) physical characterization of oxygen mass transfer efficiency and mixing intensity, 2) batch cultivation of Escherichia coli BL21 for comparison of growth characteristics, and 3) batch cultivation of recombinant E. coli BL21 expressing a clinical therapeutic, hCD83ext (the extracytoplasmic domain of human CD83). Oxygen mass transfer (evaluated as the mass transfer coefficient, kLa) was comparable between the 1D RB and STR (approximately 150 h-1) at low working volume (WV), declining linearly with increasing WV, while kLa was highest in the 2D RB for all tested WVs, providing the maximum kLa (394 h-1) at 3 L WV. Fast mixing (t95 of 8-20 s) was observed in all three systems for water and aqueous carboxymethylcellulose (CMC) solutions. Batch growth characteristics of E. coli BL21 were similar in each system, although acetate accumulation was significant in the 1D RB. Batch production of GST-hCD83ext (glutathione S-transferase-hCD83ext fusion protein) resulted in similar soluble protein yields and inclusion body formation between bioreactors. Although cell growth and protein expression were comparable between all bioreactors, the 1D RB is not considered a suitable cultivation system for E. coli under experimental conditions given the significant acetate accumulation observed and high supplemental oxygen requirement for low cell density cultures. On the other hand, considering its formidable mass transfer capacity and overall performance in batch cultivations, the CELL-tainer® is an attractive alternative to the STR for cultivation of recombinant E. coli expressing high value therapeutic proteins.
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Book chapters on the topic "Rocking bioreactor"

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Oosterhuis, Nico M. G. "Perfusion Process Design in a 2D Rocking Single-Use Bioreactor." In Continuous Processing in Pharmaceutical Manufacturing. Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527673681.ch07.

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Conference papers on the topic "Rocking bioreactor"

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"Adaptation of the sensor method for the determination of mixing time in a rocking single-use bioreactor." In Chemical technology and engineering. Lviv Polytechnic National University, 2021. http://dx.doi.org/10.23939/cte2021.01.166.

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