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Journal articles on the topic 'Rooting cuttings'
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1
Davis, Tim D., Steven W. George, Abha Upadhyaya, and Jerry Parsons. "Propagation of Firebush (Hamelia patens) by Stem Cuttings." Journal of Environmental Horticulture 9, no. 2 (June 1, 1991): 57–61. http://dx.doi.org/10.24266/0738-2898-9.2.57.
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Abstract The influence of bottom heat, rooting medium, type of cutting (i.e. terminal vs. subterminal), and commercial auxin formulations on greenhouse propagation of firebush (Hamelia patens Jacq.) by leafy stem cuttings during winter was investigated. Without bottom heat, mid-day rooting medium temperature averaged about 22 ± 3°C (72 ± 5°F). Percent rooting of auxin-treated cuttings without bottom heat was 50% and mean length of the longest root and visual rating scores of root development were low. Maintaining the rooting medium at 29–39°C (85–10 1°F) increased percent rooting for auxin-treated cuttings to 96–100% and increased root length and visual rating scores several-fold. Rooting percentage, root length, and visual rating scores were consistently high in perlite and low in peat moss. Terminal stem-tip cuttings and subterminal stem segment cuttings with basal stem diameters of 3–5 mm (0.13–0.20 in) rooted slightly better than subterminal stem segment cuttings which had basal stem diameters of 6–8 mm (0.25–0.32 in.). Terminal stem-tip cuttings not treated with auxin but with bottom heat had rooting percentages of 81–86%. Treatment of terminal stem-tip cuttings with Rootone F or Wood's Rooting Compound (1:20 or 1:5 dilution) generally resulted in rooting percentages above 90%. Some of the auxin treatments also resulted in increased root length and visual rating scores. Despite these differences, none of the plants grown from auxin-treated cuttings, were distinguishable from plants grown from nontreated cuttings two months after the rooting period. Of the variables studied, bottom heat had the most dramatic effect on rooting of stem cuttings during winter months.
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Henry, Paul H., Frank A. Blazich, and L. Eric Hinesley. "Vegetative Propagation of Eastern Redcedar by Stem Cuttings." HortScience 27, no. 12 (December 1992): 1272–74. http://dx.doi.org/10.21273/hortsci.27.12.1272.
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Studies were conducted to investigate the effects of season (timing), IBA application, genotype, crown position, type of cutting (straight vs. heel), cutting length, and stock plant age upon adventitious rooting of stem cuttings of eastern redcedar (Juniperus virginiana L.). Genotype had a strong influence on percent rooting, root count, and root length of 4-year-old trees. With trees of this age, percent rooting was maximized (87%) with hardwood cuttings taken in January and treated with 5000 ppm IBA. Crown position from which cuttings were collected did not influence rooting. Straight cuttings, with or without a light wound, rooted at a significantly higher percentage (78%) than heel cuttings (52%). With 30-year-old trees, cuttings from the lower third of the crown rooted at a significantly higher percentage (67%) than cuttings from the middle third (43%). Better rooting was obtained with straight (68%) than heel (47%) cuttings. Cutting length affected rooting, with root count and length highest in longer cuttings. Increased tree age reduced rooting, although cuttings from 40-year-old trees retained substantial rooting capacity. Chemical name used: 1 H-indole-3-butyric acid (IBA).
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Jurásek, A., and J. Martincová. "Possibilities of influencing the rooting quality of Norway spruce (Picea abies [L.] Karst.) cuttings." Journal of Forest Science 50, No. 10 (January 11, 2012): 464–77. http://dx.doi.org/10.17221/4642-jfs.
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The influence of the date of cutting collection and cutting position in the crown on rooting quality was evaluated in cuttings taken from seven-year ortets. The evaluation of various dates of cutting collection in spring demonstrated a possibility of successful propagation by cuttings during a relatively long period from full bud dormancy to flushing onset. The relationship between the development of aboveground parts (flushing, shoot and bud formation) and rooting quality was not established. The exposure of cuttings in the crown of parent tree with respect to the cardinal points did not influence rooting percentage and quality. A somewhat higher rooting ability was observed in cuttings from lower parts of the crown in comparison with cuttings taken from the highest whorls. Differences were more perceivable in generally weak-rooting clones. The comparison of rooting quality in cuttings from seven- and eight-year mother plantations with cuttings from a sixteen-year mother plantation confirmed that not only the rooting of cuttings collected from older mother plantations is weaker but also their growth is slower and their plagiotropic growth continues for a longer time.
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AI-Tamimi, Osama M., and Mostafa M. QrunBeh. "Propagation of GF677 Peach Rootstock by Stem Cuttings." HortScience 31, no. 4 (August 1996): 566c—566. http://dx.doi.org/10.21273/hortsci.31.4.566c.
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The rooting ability of GF677 peach rootstock by hardwood (H), semihardwood (SH), and softwood (S) stem cuttings collected January, February, May, and November, treated with various IBA concentrations—0, 500, 1000, 2000, and 3000 ppm—was studied. H cuttings collected in January and treated with 2000 ppm IBA caused significant increase in rooting (62%–5%). In addition, the SH cuttings prepared February and treated with 1000 or 2000 ppm IBA gave 42.5%. The May experiment resulted in low rooting percentage where H cuttings treated with 500 ppm IBA gave 10% rooting. In October, SH cuttings with 1000 or 3000 ppm IBA gave the highest rooting percentage (60%), while in November 90% rooting was obtained in H cuttings treated with 3000 ppm. Regardless of type of cutting, IBA at 1000, 2000, or 3000 ppm was better for rooting the GF677 than at 500 ppm. However, irrespective of IBA concentration, H and SH cuttings gave significantly high rooting percentages. On the other hand, best rooting was obtained when the stem cuttings of GF677 (regardless of wood type) were collected in November. Wounding base of the cutting of GF677 improved rooting ability.
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Scagel, C. F. "Enhanced Rooting of Kinnikinnick Cuttings using Mycorrhizal Fungi in Rooting Substrate." HortTechnology 14, no. 3 (January 2004): 355–63. http://dx.doi.org/10.21273/horttech.14.3.0355.
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Hardwood cuttings of kinnikinnick (Arctostaphylos uva-ursi `Massachusetts') were inoculated with three different types of inoculum of mycorrhizal fungi to determine whether addition of mycorrhizal inoculum into the rooting substrate during cutting propagation increases rooting or root growth, or alters the time for rooting. Cuttings, treated or untreated with rooting hormone prior to sticking into the rooting substrate, were inoculated with either inoculum of an arbuscular mycorrhizal fungus (AMF), hyphal inoculum of an arbutoid mycorrhizal fungus (E), or inoculum consisting of colonized root fragments of kinnikinnick (R). Cuttings were placed under mist in a greenhouse with no bottom heat and harvested 35, 56, and 84 days after sticking. Using AMF inoculum in the rooting substrate did not enhance rooting of cuttings, while adding the R or E inoculum to the rooting substrate increased root initiation compared to non-inoculated cuttings. Cuttings inoculated with either the R or E inoculum had greater root initiation than non-inoculated cuttings 56 and 84 days after sticking. When treated with rooting hormone, cuttings inoculated with the E or R inoculum had longer roots and a greater root biomass than non-inoculated cuttings. Mycorrhizal colonization of roots was similar or greater when cuttings were inoculated with the E inoculum than with the R inoculum and application of rooting hormone generally increased root colonization. The use of inoculum composed of root fragments from kinnikinnick during cutting propagation does not appear to be more beneficial than use of hyphal inoculum from a known arbutoid mycorrhizal fungus.
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Li, Zhaohui, Yan Ma, Wanyuan Yin, Dekui Zang, and Xianfeng Guo. "Exploring Vegetative Propagation Techniques for the Threatened Pteroceltis tatarinowii Maxim Using Stem Cuttings." HortScience 54, no. 4 (April 2019): 721–24. http://dx.doi.org/10.21273/hortsci13729-18.
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Pteroceltis tatarinowii Maxim, the only species of the genus Pteroceltis (family Ulmaceae), is an endemic rare tree species in China. This study was performed to explore vegetative propagation techniques for P. tatarinowii using stem cuttings. First, the effects of exogenous indole-3-butyric acid (IBA) and cutting positions on rooting performance were investigated to screen the appropriate exogenous auxin treatment and to determine the proper cuttings type. The results showed that the control cuttings pretreated with no exogenous IBA, irrespective of whether the stem cuttings were terminal, middle, or basal, rooted in a manner significantly inferior to that of cuttings pretreated with IBA. Their rooting percentage was less than 50%. Among the IBA-treated cuttings, the middle cuttings pretreated with 1000 mg·L−1 IBA rooted best, with the shortest number of days until rooting emergence (20 days), the highest rooting percentage (84.0%), the lowest mortality rate (4.0%), the greatest root number (average of 6.7 per cutting), and the longest roots per cutting (44.4 cm per cutting). Terminal cuttings pretreated with 1000 mg·L−1 IBA acquired satisfactory rooting traits and had the same shortest rooting duration (20 days) and the following parameters: rooting, 70.7%; mortality, 10.7%; average roots per cutting, 5.2; and longest root, 29.1 cm. To further determine the optimum cutting propagation time for this plant, a second experiment was performed and the cuttings were collected beginning in early June, when the growth of the current season was feasible for harvesting cuttings. Stem cuttings collected in late June and middle July had significantly higher rooting percentages (≥80%) compared with those collected in early June (66.7%). The other three rooting parameters were not significantly affected by the collection date. However, according to the overall rooting traits, the cuttings collected in both late June and middle July remarkably outperformed those collected in early June regarding the number of roots and the total root length per cutting. The initial nutrient reserves in the cuttings were also determined. A significant difference in the soluble carbohydrate level was found among collection times, but the nitrogen level in the cuttings was similar. The study revealed that stem cutting propagation of P. tatarinowii was achievable, and it was best achieved with cuttings collected from the terminal and middle positions of the branches of the current season from late June to middle July and treated with 1000 mg·L−1 IBA using the quick dip method.
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An, Haishan, Jiajia Meng, Fangjie Xu, Shuang Jiang, Xiaoqing Wang, Chunhui Shi, Boqiang Zhou, Jun Luo, and Xueying Zhang. "Rooting Ability of Hardwood Cuttings in Highbush Blueberry (Vaccinium corymbosum L.) Using Different Indole-butyric Acid Concentrations." HortScience 54, no. 2 (February 2018): 194–99. http://dx.doi.org/10.21273/hortsci13691-18.
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Vegetative propagation by cuttings is a very popular method. However, blueberry propagation using cuttings is still a main factor limiting its expansion because its results can vary according to the blueberry cultivar and environmental factors. This study aimed to evaluate the rooting abilities of hardwood cuttings for six blueberry cultivars (O’Neal, Misty, Diana, Biloxi, Bluebeauty, and Coville) using three different exogenous indole-butyric acid (IBA) concentrations (1000, 2000, and 3000 ppm), and to determine if the cutting position (basal, central, apical) affects rooting performance. A control treatment (0 ppm IBA) was also performed. After 90 days of each treatment, rooting percentage, average root length, and average root number per cutting were assessed and used to calculate rooting index, which is a measure of rooting ability. The rooting percentages of hardwood cuttings differed largely among cultivars and were highest for ‘Bluebeauty’ (68.55%), followed by ‘Biloxi’ (68.01%). The rooting index values of these two cultivars (33.59 and 35.18, respectively) were significantly higher than those of the other four cultivars. The rooting response of blueberry hardwood cuttings to IBA concentrations was quadratic, and 1000 and 2000 ppm IBA were sufficient to express the maximum rooting percentage in most cultivars. The rooting abilities of basal, central, and apical cuttings were similar with treatments with high IBA concentrations. The effects of the cultivar, IBA concentration, and interaction between them on rooting percentage, average root length, and average root number were significant; however, the effects of the cutting position on the rooting percentage and average root length were not. This suggested that the rooting abilities of blueberry hardwood cuttings were significantly influenced by the cultivar and IBA concentration rather than by the cutting position.
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Markovic, Marija, Dragana Skocajic, Mihailo Grbic, and Matilda Djukic. "Effects of the time of cuttings collection and IBA concentration on the rooting of softwood cuttings from elite trees of Cornelian cherry (Cornus mas L.) in Belgrade area." Bulletin of the Faculty of Forestry, no. 110 (2014): 105–20. http://dx.doi.org/10.2298/gsf1410105m.
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In this study, the effect of concentration of indole-3-butyric acid (IBA) (powder dip), cutting type as well as the time of taking cuttings on the rooting of softwood cuttings of cornelian cherry was examined. Four types of cuttings were used: basal cuttings, terminal cuttings, basal cuttings with 2-year-old wood and terminal cuttings with 2-year-old wood. The obtained results showed that IBA concentration, cutting type and time of collecting have significant effect on rooting. The best results were obtained using 1% IBA and cuttings collected in the second term (in mid-July) had a higher rooting percentage. Terminal cuttings treated with 1% IBA (powder dip) should be used for optimum results. In that case the rooting percentage was very high (over 90%) in both terms of cuttings collection.
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SABATINO, Leo, Fabio D’ANNA, and Giovanni IAPICHINO. "Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 42, no. 2 (December 2, 2014): 478–81. http://dx.doi.org/10.15835/nbha4229611.
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Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.
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Atangana, A. R., and D. P. Khasa. "Preliminary survey of clonal variation in rooting of Allanblackia floribunda leafy stem cuttings." Canadian Journal of Forest Research 38, no. 1 (January 2008): 10–15. http://dx.doi.org/10.1139/x07-151.
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Clonal and cutting stem morphology variations in rooting ability were simultaneously examined in Allanblackia floribunda Oliv. using leafy stem cuttings in two experiments. The hypothesis tested is that clonal variation in rooting percentage is due to variation in cutting morphology. Each of the four replicate blocks used per experiment consisted of 18 treatments from two crossed factors, diameter or length × clone, making a total of n = 216 cuttings (3 cutting lengths (3, 4, and 5 cm) or diameters (2, 3, and 4 mm) × 6 stumps (clones) × 12 cuttings, as each experimental unit consisted of 12 cuttings). The cuttings were used for rooting in nonmist propagators. No clone × cutting diameter and clone × cutting length interactions (P = 0.293 and 0.513, respectively) on rooting percentage were found at the end of the study. Highly significant and significant clonal variations in rooting percentage were noted throughout the experiments. Results from this study reject the hypothesis being tested. Finally, further work should be done on the effects of physiological status of the cuttings and clonal variation in this species.
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Griffin, Jason J., Frank A. Blazich, and Thomas G. Ranney. "Propagation of Thuja x ‘Green Giant’ by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment." Journal of Environmental Horticulture 16, no. 4 (December 1, 1998): 212–14. http://dx.doi.org/10.24266/0738-2898-16.4.212.
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Abstract Stem cuttings of Thuja L. x ‘Green Giant’ (‘Green Giant’ arborvitae), consisting of 22 cm (8.6 in) terminals (tips of first-order laterals) or 20 cm (7.8 in) laterals (side shoots removed from those portions of terminal cuttings inserted into the rooting medium), were collected on three dates associated with specific stock plant growth stages (softwood, semi-hardwood, and hardwood) and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm (0.9%). Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting for softwood, semi-hardwood, and hardwood, cuttings was 85%, 86%, and 96%, respectively. Semi-hardwood cuttings were the only cuttings in which percent rooting was affected by IBA treatment or cutting type, with lateral cuttings rooting in higher percentages than terminal cuttings (92% vs. 79%, respectively), while IBA concentrations of 3000 (0.3%) or 6000 ppm (0.6%) proved optimal for both cutting types. Mean root number and mean root length varied by growth stage, cutting type, and IBA concentration. In general, rooting percentages and root length were optimized at the hardwood stage with either cutting type treated with 3000, 6000 or 9000 ppm (0.3%, 0.6% or 0.9%) IBA, resulting in >10 roots per cutting.
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Costa Junior, Euvaldo De Sousa, Mayara Suzane de Melo Barbosa, Cândida Maria Anjos da Silva, Raíra Carine Santana da Silva, Lucia Helena Piedade Kiill, and Márkilla Zunete Beckmann-Cavalcante. "Vegetative propagation of Rhaphiodon echinus Schauer (Lamiaceae): effects of the period of cutting in rooting, cuttings arrangement and IBA concentrations for seedlings production." Ornamental Horticulture 24, no. 3 (September 25, 2018): 238–47. http://dx.doi.org/10.14295/oh.v24i3.1232.
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One of the tendencies in the floriculture sector, whether in modern landscaping or floral art, is related to the insertion of innovations in the sector, mainly of native origin. For this it is necessary to establish strategies that favor the introduction of these materials. Among the species that present ornamental potential, Rhaphiodon echinus Schauer has been outstanding for groundcover; however it is necessary to establish the proper propagation protocol for the species. In this sense, the objective was to evaluate the vegetative propagation process of this species considering the period of cutting in rooting, cuttings arrangement and IBA concentrations for seedlings production. The experiment was carried out in a sub-split plots scheme with the period of cutting in rooting in the plots (30 and 60 days), the cuttings arrangement (horizontal and vertical) in the subplots and the IBA concentrations (0; 1,000; 2,000 and 4,000 ppm) in sub-subplots. The following variables were evaluated: survival cuttings, percentage of rooted cuttings, number of sprouts per cuttings, average length of the largest root, root volume, shoot dry biomass and root dry biomass. There was a significant interaction between the period of cutting in rooting and the cuttings arrangement for survival cuttings and rooted cuttings; between the period of cutting in rooting and the concentration of IBA for shoot dry and between the cuttings arrangement and concentration and IBA for the percentage of rooted cuttings, number of sprouts per cuttings and root dry biomass. For average length of the largest root and root volume there was a significant effect for period of cutting in rooting and IBA concentrations individually. The period of cutting in rooting of 30 days is the most suitable for rooting the cuttings. In relation of the cuttings arrangement the most indicated is vertical. Finally, the concentration of 1,000 ppm of IBA can be indicated for the propagation of R. echinus by the cutting process.
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Tsipouridis, C., T. Thomidis, and Z. Michailides. "Influence of some external factors on the rooting of GF677, peach and nectarine shoot hardwood cuttings." Australian Journal of Experimental Agriculture 45, no. 1 (2005): 107. http://dx.doi.org/10.1071/ea03120.
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The influence of some external factors [cultivar, indole-butyric acid (IBA) concentration, IBA dipping of shoot tip and base, preheating of cuttings, collecting date, time of cutting collection, mother tree age, cultivar, length of and diameter of cutting] on rooting of peach–nectarine hardwood shoots in the field were examined. It was found that the best IBA concentration for rooting differed among peach cultivars. Rooting of cuttings whose bases only were immersed in IBA solution was higher than those that had both base and tip immersed. In addition, a higher percentage of rooting shoots was achieved with preheating of cuttings before planting. The best length for cuttings varied between cultivars and the best diameter was 10–13 mm. Cuttings collected between 25 October and 13 November (1997 and 1998) showed the highest percentage of rooting shoots. There was also a higher percentage of rooting GF677 hardwood shoot cuttings collected from 7-year-old mother trees compared with those collected from 1-year-old mother trees.
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Carratu, Michelle, and Roger J. Sauve. "873 PB 229 ROOTING STUDIES ON POINSETTIAS." HortScience 29, no. 5 (May 1994): 559a—559. http://dx.doi.org/10.21273/hortsci.29.5.559a.
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Several studies were conducted to evaluate the effects of cultivar, cutting length, and leaf number on rooting of poinsettia. Cuttings were rooted under mist in a soilless medium with 50 cuttings per treatment. Visual rootball ratings were performed after 3 wk. In the first experiment, rooting of ten poinsettia cultivars was compared. The rooting hormone was 0.1% indole-3-butyric acid (IBA). Rooting of `V-14 Red' and `V-14 Marble' was the highest. `V-17 Pink' and `V-17 Marble' had the highest number of callused cuttings. `V-17 White' produced the highest number of extensively rooted cuttings. `V-14 Pink' (3-lf) cuttings 12 cm long rooted better than 5 cm cuttings. Rooting of (7 cm) 3- and 4-leaf cuttings was higher than rooting of 2-leaf cuttings. `V-14 Pink' cuttings treated with 0.8% IBA or 1% IBA + 0.5% 1-napthaleneacetic acid (NAA) rooted better than with 0.1% or 0.3% IBA.
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Grönroos, Roland, and Sara von Arnold. "Initiation of roots on hypocotyl cuttings of Pinussylvestris, with emphasis on direct rooting, root elongation, and auxin uptake." Canadian Journal of Forest Research 18, no. 11 (November 1, 1988): 1457–62. http://dx.doi.org/10.1139/x88-224.
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Direct rooting of hypocotyl cuttings of Pinussylvestris L. is described and compared with rooting via wound tissue. The optimal 3-indolylbutyric acid treatment for direct rooting of Pinussylvestris hypocotyl cuttings under in vitro conditions was 1.23 mM for 24 h. This treatment resulted in 45% rooting within 3 weeks after cutting. During this period, only direct rooting was observed. Three to 4 weeks after cutting, roots developed either directly on the hypocotyl or via wound tissue. After the first month, rooting took place predominantly via wound tissue. The optimal 3-indolylbutyric acid treatment found for in vitro culture could successfully be used for hydroponic culture (under nonsterile conditions). In vitro culture inhibited root elongation. However, after transfer of rooted cuttings from in vitro to hydroponic culture, root elongation increased markedly. Uptake studies with 3-[5(n)-3H]indolylacetic acid mixed with the nutrient solution showed that auxin uptake by the cutting probably occurred mainly through uptake of the nutrient solution. After 24 h treatment with 1.43 mM 3-indolylacetic acid, the concentration taken up by the cuttings was 0.91 nmol/mg fresh weight. Differences between individual cuttings in auxin uptake could not explain the poor direct rooting of Pinussylvestris hypocotyl cuttings cultured in vitro.
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Zem, Luciele Milani, Katia Christina Zuffellato-Ribas, Maria Izabel Radomski, and Henrique Soares Koehler. "Rooting of semihardwood cuttings of cataia collected in four seasons." Ciência Rural 45, no. 10 (July 10, 2015): 1815–18. http://dx.doi.org/10.1590/0103-8478cr20140534.
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Drymis brasiliensis Miers is a specie of the Winteraceae family, commonly known as cataia or pepper bark. This research was carried out with the aim to study the rooting of semihardwood cuttings, subjected to different concentrations of indolebutyric acid (IBA), collected in four seasons of the year (autumn, winter, spring and summer). Cuttings were prepared with 10-12cm length, keeping two halved leaves. Bases of cuttings were treated as follows: control (100% water), 0; 500; 1500; 3000; 4500 and 6000mg L-1 IBA.One hundred and twenty after the planting the following variables were assessed: rooting percentage, number of roots per cutting, length of roots per cutting, cutting callus percentage, cuttings with new shoots and number of cuttings that maintained the initial leaves. The indolebutyric acid is not recommended for rooting of Drimys brasiliensis cuttings. The cuttings collected in winter were the ones that showed greatest rooting percentage (46.96%) for the production of nursery plant of Drimys brasiliensis.
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Gulen, Hatice, Yasar Erbil, and Atilla Eris. "Improved Rooting of Gisela-5 Softwood Cuttings following Banding and IBA Application." HortScience 39, no. 6 (October 2004): 1403–5. http://dx.doi.org/10.21273/hortsci.39.6.1403.
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A stock plant etiolation treatment was tested to improve rooting of the important cherry rootstock Gisela-5. To create the etiolation effect, at the beginning of the growing season, banding (blanching) was initiated on stock plants by placing black plastic tape at the base of new shoots for 6 or 10 weeks. Cuttings were excised so that the banded area was at the cutting base. IBA was applied at two concentrations (5 and 10 mm) to the cutting base following wounding and cuttings were placed in perlite (100%) rooting medium under mist. The rooting percentage, number of roots per cutting and root length were measured 4 weeks after planting. Banding and duration significantly stimulated rooting of leafy softwood cuttings. The highest rooting percentage (80.0%) was obtained on cuttings banded for 6 weeks and treated with 5 mm IBA.
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Dolkar, Phuntsog, Phuntsok Angmo, Diskit Dolkar, Bhuvnesh Kumar, Om Praksah Chaurasia, and Tsering Stobdan. "Effect of Mulching, Shading, Spacing and Cutting Thickness on Propagation of Seabuckthorn (Hippophae rhamnoides L.) by Cuttings." Defence Life Science Journal 3, no. 1 (December 15, 2017): 75. http://dx.doi.org/10.14429/dlsj.3.12093.
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<p>The effect of plastic mulching, coloured shade netting, spacing and cutting thickness on rooting success and growth of Seabuckthorn (Hippophae rhamnoides L.) through hard wood cuttings is addressed. Use of silver black plastic mulching film resulted in 10 per cent higher rooting success and significant plant growth. Suppression of weed emergence by the plastic mulch resulted in 75.8 per cent time saving in manual weeding by farm workers. No significant gender difference in rooting success was observed. Reduction in light intensity by 66 per cent using green shade net resulted in significant reduction in rooting and growth of nursery plants. Three different spacing between cuttings did not show significant difference in rooting and growth related parameters suggesting that cuttings can be planted denser (3"×3") under mulching to get higher number of nursery stock per unit area. Cutting thickness showed significant effect on rooting success. Highest rooting percentage was observed in pencil thickness cuttings (7.5 ± 1.6 mm dia) followed by cuttings with 2.9 ± 0.8 mm and 11.3 ± 1.7 mm basal diameter. The result of the present study could facilitate establishment of a vegetative propagation method wherein faster growth and larger number of cuttings can be propagated with higher rooting success rate.</p>
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Cunliffe, Bruce A., Mary Hockenberry Meyer, and Peter D. Ascher. "Propagation of Pennisetum setaceum ‘Rubrum’ from Cuttings." Journal of Environmental Horticulture 19, no. 1 (March 1, 2001): 1–3. http://dx.doi.org/10.24266/0738-2898-19.1.1.
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Abstract The effect of rooting medium, indole-3-butyric acid (IBA), and node position were studied on rooting of tender purple fountaingrass, Pennisetum setaceum ‘Rubrum’. Three-hundred single-node culm cuttings were placed in a mist house in sand, peat, vermiculite, perlite, or a peat:perlite (1:1 by vol) mix. Within 42 days, 78% of the cuttings rooted, with the highest number of cuttings rooting in the peat or perlite media and the lowest number in the vermiculite medium. Cuttings from the most proximal node exhibited a significantly higher percentage of rooting and produced more roots than cuttings from more distal nodes. Rooting medium and nodal position of cutting interacted to affect root dry weight. IBA had no significant effect on rooting. Propagation of purple fountaingrass from culm cuttings offers an economically-attractive alternative to the conventional method of crown division.
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LeBude, Anthony V., Barry Goldfarb, Frank A. Blazich, John Frampton, and Farrell C. Wise. "Mist Level Influences Vapor Pressure Deficit and Gas Exchange During Rooting of Juvenile Stem Cuttings of Loblolly Pine." HortScience 40, no. 5 (August 2005): 1448–56. http://dx.doi.org/10.21273/hortsci.40.5.1448.
Full textAbstract:
Two experiments were conducted during which juvenile hardwood or softwood stem cuttings of loblolly pine (Pinus taeda L.) were rooted under six mist regimes in a polyethylene-covered greenhouse to investigate the effect of mist level on vapor pressure deficit (VPD) and cutting water potential (Ψcut), and to determine the relationships between these variables and rooting percentage. In addition, net photosynthesis at ambient conditions (Aambient) and stomatal conductance (gs) were measured in stem cuttings during adventitious root formation to determine their relationship to rooting percentage. Hardwood stem cuttings rooted ≥80% when mean daily VPD between 1000 and 1800 hr ranged from 0.60 to 0.85 kPa. Although rooting percentage was related to Ψcut, and Aambient was related to Ψcut, rooting percentage of softwood stem cuttings was not related to Aambient of stem cuttings. Using VPD as a control mechanism for mist application during adventitious rooting of stem cuttings of loblolly pine might increase rooting percentages across a variety of rooting environments.
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Cartabiano, Julia A., and Jessica D. Lubell. "Propagation of Four Underused Native Species from Softwood Cuttings." HortScience 48, no. 8 (August 2013): 1018–20. http://dx.doi.org/10.21273/hortsci.48.8.1018.
Full textAbstract:
Interest in native plants for landscaping is increasing and nursery growers must expand their product offerings by adding new native species. Softwood stem cutting propagation of four underused northeastern U.S. native species [Ceanothus americanus (L.), Corylus cornuta (Marsh.), Lonicera canadensis (Bartr.), Viburnum acerifolium (L.)] was studied. V. acerifolium cuttings containing two nodes taken mid-June to mid-August rooted at nearly 100% with at least 15 roots per cutting. Exogenous auxin application did not enhance rooting of two-node V. acerifolium cuttings. Single-node V. acerifolium cutting success and quality of rooting increased with increasing concentration of auxin applied and reached a maximum of 80% rooting, whereas untreated cuttings only rooted at 53%. C. cornuta cuttings taken mid-June to mid-August rooted at greater than 85%. Hormone concentration did not affect rooting percentage for C. cornuta; however, cuttings treated with 3000 and 8000 ppm indole-3-butyric acid (IBA) had more and longer roots than untreated cuttings. June was the optimal time to collect cuttings of C. americanus (57% rooting) and L. canadensis (49% rooting), and rooting hormone did not significantly impact propagation success. C. cornuta and V. acerifolium could be propagated at a level necessary for consideration as a new commercial crop by general wholesale nurseries looking to add select native shrubs to their product lines. All four species evaluated could be viable commercial crops for nurseries that specialize in native plants.
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Scagel, C. F. "Cultivar Specific Effects of Mycorrhizal Fungi on the Rooting of Miniature Rose Cuttings." Journal of Environmental Horticulture 19, no. 1 (March 1, 2001): 15–20. http://dx.doi.org/10.24266/0738-2898-19.1.15.
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Abstract The benefits from root colonization by mycorrhizal fungi are thought to be highest when colonization occurs as early as possible during plant growth. We assessed whether addition of VA mycorrhizal fungi (VAMF) inoculum into rooting medium during cutting propagation would increase the quantity of rooting and the quality of rooted cuttings for five different cultivars of miniature roses (Rosa spp.). Four weeks after cuttings were stuck, the number of cuttings with roots for two cultivars that normally take longer to root, increased with addition of VAMF inoculum into the rooting medium. The combination of hormone treatment (IBA and NAA) and VAMF inoculum in the rooting medium increased the number of rooted cuttings and the number of roots per cutting for three cultivars when compared to cuttings that only received hormone treatment. Increases in root initiation and root growth of cuttings rooted in medium containing VAMF inoculum were not always associated with increased levels of root colonization by VAM fungi. Our results indicate that although adding VAMF inoculum into the rooting medium does not always increase root initiation, in some cultivars the combination of VAMF inoculum and rooting hormones can increase root initiation and potentially increase the quality of rooted cutting produced.
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Osterc, G., M. Štefančič, A. Solar, and F. Štampar. "Potential involvement of flavonoids in the rooting response of chestnut hybrid (Castanea crenata × Castanea sativa) clones." Australian Journal of Experimental Agriculture 47, no. 1 (2007): 96. http://dx.doi.org/10.1071/ea05149.
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The involvement of different quercetins [quercetin-3-O-rhamnoside (quercitrin), quercetin-3-D-galactoside (hyperoside) and rutin] and catechins (catechin, catechol) in the rooting process of leafy cuttings was studied in two hybrid chestnut (Castanea crenata × Castanea sativa) clones, Maraval and Marsol. Both clones differed strongly in rooting results. The Maraval clone cuttings, which had a high rooting rate, contained, on average, higher amounts of all quercetins in different plant parts (leaves and basal cuttings) than the Marsol clone, which had a low rooting rate. There was a highly significant correlation between the quercetin contents of the cutting leaves and the rooting process (number of main roots). The catecechin contents of the cutting leaves did not show any correlation with the rooting process.
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Blythe, Eugene K., Jeff L. Sibley, Ken M. Tilt, and Bertram Zinner. "Monolithic Slag as a Substrate for Rooting and Bare- Rooting Stem Cuttings." Journal of Environmental Horticulture 23, no. 2 (June 1, 2005): 67–71. http://dx.doi.org/10.24266/0738-2898-23.2.67.
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Abstract Stem cuttings of Hydrangea paniculata Sieb., Rosa L. ‘Red Cascade’, Salvia leucantha Cav., and Solenostemon scutellarioides (L.) Codd ‘Roseo’ were inserted into six rooting substrates: monolithic slag, sand, perlite, vermiculite, Fafard 3B, or fine pine bark. Rooting, initial shoot growth, and ease of dislodging substrate particles from root systems upon bare-rooting by shaking and washing cuttings rooted in monolithic slag were compared to cuttings rooted in the five other substrates. Rooting percentage, number of primary roots per rooted cutting, and total root length per rooted cutting for cuttings rooted in monolithic slag were generally similar to the five other substrates. Particles of monolithic slag were dislodged more readily from root systems by shaking than were the other substrates. Gentle washing removed almost all particles of monolithic slag and sand from the root systems of all taxa and removed almost all particles of pine bark from all taxa except S. scutellarioides ‘Roseo’. Monolithic slag had a bulk density similar to sand, retained less water than the other substrates, and was similar to perlite, vermiculite, and pine bark in particle size distribution.
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Crawford, Brigitte D., John M. Dole, and Ben A. Bergmann. "Influences of Season and Cutting Week within a Propagation Cycle on Rooting of ‘Stained Glass’ Coleus Shoot Tip Cuttings Are Not Overcome by Rooting Compound Treatment." HortTechnology 26, no. 5 (October 2016): 620–27. http://dx.doi.org/10.21273/horttech03464-16.
Full textAbstract:
Influence of season of the year, cutting week within a propagation cycle (number of weeks from which a stock plant has been harvested), stock plant age, and rooting compound on postpropagation cutting quality, and adventitious rooting was examined for ‘Stained Glass’ coleus (Solenostemon scutellarioides). Cuttings were of higher quality and produced more robust root systems when a propagation cycle started in summer vs. fall or spring even when cuttings were harvested from stock plants of the same age. Cutting week within a propagation cycle significantly influenced postpropagation cutting quality and rooting when cuttings were harvested over many weeks from the same stock plants and when cuttings were harvested for three propagation events using stock plants of different ages. When cuttings were harvested on the same days from stock plants of three distinct ages, cuttings harvested in the first week were larger with greater root weights but had more yellowed leaves and lower quality ratings compared with the two subsequent cutting weeks, but stock plant age had no effect on any observed parameter. Treatment with rooting compound did not overcome the significant influences of season and cutting week within a propagation cycle whether rooting was carried out in a greenhouse or growth chamber. Shoot and root fresh and dry weights were positively correlated with both daylength and midday instantaneous light of the stock plant environment.
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Azad, Md Salim, and Md Abdul Matin. "Effect of Indole-3-Butyric Acid on Clonal Propagation of Swietenia macrophylla through Branch Cutting." Journal of Botany 2015 (November 5, 2015): 1–7. http://dx.doi.org/10.1155/2015/249308.
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The study discloses the scopes of clonal propagation Swietenia macrophylla through branch cutting treated with IBA. A total of four hundred cuttings were used with four replications to assess the rooting ability. The study exposed significant (p<0.5) difference of rooted cuttings among the treatments. The highest (62.51%) rooting percent was observed in cutting with 0.4% IBA treatment. In addition, root number and its length per cutting were increased with increasing IBA concentration (p<0.5). The experiment showed significant (p<0.5) difference of sprouting among the treatments. The highest (67.27%) percent of sprouting was observed in cuttings with 0.4% solution, which showed a similar fashion of percent of rooting. The study also showed significant (p<0.5) difference of shoot number per cutting, the length of the longest shoot, and number of leaves per cutting among the treatments. The overall survival of rooted cuttings after transfer to polybag significantly (p<0.5) differed among the cuttings treated with different IBA solution. The highest survival percentage (69.67%) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (55.6%) survival was found in cuttings treated with control. The use of 0.4% IBA treatment is suggested for rooting of juvenile leafy branch cutting of S. macrophylla.
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Naalamle Amissah, J., and Nina Bassuk. "Cutting Back Stock Plants Promotes Adventitious Rooting of Stems of Quercus bicolor and Quercus macrocarpa." Journal of Environmental Horticulture 27, no. 3 (September 1, 2009): 159–65. http://dx.doi.org/10.24266/0738-2898-27.3.159.
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Abstract Studies were conducted to investigate the severity of cutting back stock plants on adventitious rooting of layered stems and stem cuttings of Quercus bicolor Willd. (swamp white oak) and Quercus macrocarpa Michx. (bur oak). Rooting averaged 77% in Q. bicolor and 70% in Q. macrocarpa layered stems from the cutback stock plant group, compared to air layered stems from intact plants which averaged 1% in Q. bicolor and 0% in Q. macrocarpa. In another experiment cuttings were taken from three stock plant heights [4 cm (1.6 in), or 100 cm (39.4 in) stumps and intact plants ∼ 170 cm (66.9 in)] which were either etiolated or grown in natural light and rooted under mist. The best rooting (59%) and average number of roots (9.3) were found in etiolated Q. bicolor cuttings taken from 4 cm (1.6 in) stumps. Q. macrocarpa cuttings rooted poorly with only 7% of the cuttings rooting. By taking cuttings a week earlier for rooting (2 weeks, at the softwood stage), rooting in Q. macrocarpa was possible, with the best rooting (46%) found in etiolated cuttings taken from 4 cm (1.6 in) stumps. Overall, the highest rooting and greatest number of roots occurred in etiolated layers and cuttings from the 4 cm cutback group. Rooting generally increased with increasing extent of stock plant cutback
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Erwin, John E., D. Schwarze, and R. Donahue. "Factors Affecting Propagation of Clematis by Stem Cuttings." HortTechnology 7, no. 4 (October 1997): 408–10. http://dx.doi.org/10.21273/horttech.7.4.408.
Full textAbstract:
Effect of media type, cultivar, and indole-3 butyric acid (IBA) application on Clematis spp. stem cutting rooting was studied. Cutting survival across all treatments was highest on `Comtesse de Bouchard' and `Gypsy Queen' cuttings and lowest on `Jackmani' cuttings. Cutting survival was greatest in perlite and lowest in peat-perlite-vermiculite. IBA application increased `Jackmani' cutting survival only. Time of root emergence was longest on `Jackmani' and least on `Gypsy Queen' cuttings across treatments. Root emergence occurred first in sand and perlite and last in peat-perlite across treatments. Root dry mass on cuttings from `Jackmani' and Clematis viticella purpurea plena elegens plants were unaffected by medium type. In contrast, root dry mass on `Comtesse de Bouchard' cuttings was highest in perlite and root dry mass on `Gypsy Queen' cuttings was highest in sand, perlite, and peat-perlite-vermiculite. The best media for propagating clematis were sand and perlite. Benefits to rooting cuttings in sand or perlite were similar, except rooting cuttings in perlite resulted in higher cutting root dry mass.
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Gil, Chan-Saem, Soon-Jae Kwon, Ho-Young Jeong, Chanhui Lee, Oak-Jin Lee, and Seok-Hyun Eom. "Blue Light Upregulates Auxin Signaling and Stimulates Root Formation in Irregular Rooting of Rosemary Cuttings." Agronomy 11, no. 9 (August 28, 2021): 1725. http://dx.doi.org/10.3390/agronomy11091725.
Full textAbstract:
Irregular rooting of rosemary stem cuttings, causing differences in either stem maturation or responses to growth conditions, restricts uniform production. Here, rooting efficiency of apical, middle, and basal cuttings from rosemary stems was evaluated by controlling light conditions to prevent irregular rooting. The types of light applied to the cuttings were natural sunlight (NSL), fluorescent, red, and blue (BL) light. Among these light sources, BL significantly induced root growth of not only basal cuttings, but also apical and middle cuttings, whereas NSL induced poor root formation in apical and middle cuttings. In particular, the roots of apical cuttings exposed to BL grew twice as fast as those exposed to other types of light. The overexpression of BL-induced IAA synthetic genes confirmed the rooting patterns. IAA synthetic genes were significantly upregulated by BL in the apical and middle cuttings. Irradiating with 50 μmol photons m−2 s−1 BL resulted in similar root production levels among the cutting positions with high biomass, guaranteeing the successful production of uniform cuttings. Thus, the application of proper high-intensity BL promoted healthy, similar-quality rosemary cuttings among stem cutting positions.
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Stein, Anne, J. André Fortin, and Gilles Vallée. "Enhanced rooting of Picea mariana cuttings by ectomycorrhizal fungi." Canadian Journal of Botany 68, no. 3 (March 1, 1990): 468–70. http://dx.doi.org/10.1139/b90-062.
Full textAbstract:
Cuttings of Picea mariana (Mill.) B.S.P. and Larix decidua Mill, were rooted in a peat moss – vermiculite substrate inoculated with vegetative mycelium of two ectomycorrhizal fungi. The fungal influence on rooting was compared with auxin-treated cuttings. The symbionts stimulated rhizogenesis of P. mariana, resulting in significantly increased rooting percentage, number of roots per cutting, and average root length as compared with controls. The best rooting percentage and the highest number of roots formed per cutting was reached under the hormonal treatment. No stimulating effect by ectomycorrhizal fungi was observed on L. decidua cuttings. With L. decidua, no significant differences in rooting percentage between the four treatments were obtained. The use of indolebutyric acid significantly increased the number of roots per cutting and decreased mean root length.
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Cao, Fan, Xinwang Wang, Zhuangzhuang Liu, Yongrong Li, and Fangren Peng. "Differential Protein Analysis of Pecan Hardwood Cuttings." HortScience 54, no. 9 (September 2019): 1551–57. http://dx.doi.org/10.21273/hortsci13776-18.
Full textAbstract:
Pecan cuttings are difficult for rooting. This study describes the pecan hardwood rooting process based on anatomic characteristics to understand root formation mechanisms of pecan cuttings. The expressed proteins of different periods during the adventitious rooting process of pecan seedling hardwood cuttings were identified and analyzed to evaluate the rooting mechanism. The expressed proteins of pecan cutting seedlings were also compared with other cultivar cuttings during the rooting period. Pecan seedling cuttings were developed at different air and substrate temperatures to induce root formation. Adventitious root formation of pecan hardwood cuttings was described, and the phloem at the base of the prepared cuttings was selected as the sample for the differential protein analysis. The results showed that adventitious root formation of pecan hardwood cuttings was the only product of callus differentiation, which originated from the cells of the cambium or vascular ray parenchyma. Such adventitious root primordia were developed from those calluses that formed the regenerative structure, and the expressed proteins during the adventitious rooting of pecan hardwood cutting were identified and analyzed by matrix-assisted laser desorption ionization–time of flight–mass spectrometry (MALDI-TOF-MS) to evaluate the rooting mechanism. Eight differentially expressed proteins were found in the rooting periods, and 15 differential proteins were found by comparing pecan cutting types, which were analyzed by peptide mass fingerprinting homology. The results show that the primordial cells were differentiated from the meristematic cells. Furthermore, the differentially expressed proteins contained energy metabolism proteins, adversity stress proteins, and signal transmission proteins. The energy metabolism-related proteins were adenosine triphosphate (ATP) synthase, photosynthesis-related proteins, and enolase. The adversity-stress proteins containing heat shock-related proteins and signal transmission proteins were mainly cytochrome enzymes and heme-binding proteins. Adventitious root formation of pecan cultivar hardwood cuttings was difficult. More trials should be performed from the potential aspects of high defensive protection and phloem morphologic structure.
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Hagen, Randall H., and David A. Palzkill. "ASEXUAL PROPAGATION OF `DESERT MUSEUM' HYBRID PALO VERDE AND MESQUITE SPECIES UNDER INTERMITTENT MIST." HortScience 25, no. 9 (September 1990): 1103G—1103. http://dx.doi.org/10.21273/hortsci.25.9.1103.
Full textAbstract:
The `Desert Museum' hybrid between the Blue, Foothills, and Mexican palo verdes has been well received by the public. However, it has remained unavailable due to difficulties in asexual propagation. Studies were conducted on effects of IBA cone. (0 to 10,000 ppm), cutting position along the stem, size of cutting, season, and temperature of the medium. For `Desert Museum', basal cuttings of slightly hardened new stem growth rooted much better than apical cuttings. Best rooting for apical cuttings was 79% using IBA from 2,500-5,000 ppm. Basal cuttings averaged 95% rooting and showed no response to IBA. Rooting of cuttings taken in September declined to 10% for apical and 2170 for basal cuttings averaged over all IBA levels. Six other species or hybrids of Cercidium and Parkinsonia and five of Prosopis were also rooted.
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Hagen, Randall H., and David A. Palzkill. "ASEXUAL PROPAGATION OF `DESERT MUSEUM' HYBRID PALO VERDE AND MESQUITE SPECIES UNDER INTERMITTENT MIST." HortScience 25, no. 9 (September 1990): 1103g—1103. http://dx.doi.org/10.21273/hortsci.25.9.1103g.
Full textAbstract:
The `Desert Museum' hybrid between the Blue, Foothills, and Mexican palo verdes has been well received by the public. However, it has remained unavailable due to difficulties in asexual propagation. Studies were conducted on effects of IBA cone. (0 to 10,000 ppm), cutting position along the stem, size of cutting, season, and temperature of the medium.For `Desert Museum', basal cuttings of slightly hardened new stem growth rooted much better than apical cuttings. Best rooting for apical cuttings was 79% using IBA from 2,500-5,000 ppm. Basal cuttings averaged 95% rooting and showed no response to IBA. Rooting of cuttings taken in September declined to 10% for apical and 2170 for basal cuttings averaged over all IBA levels. Six other species or hybrids of Cercidium and Parkinsonia and five of Prosopis were also rooted.
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LeBude*, Anthony V., Barry Goldfarb, and Frank A. Blazich. "Mist, Vapor Pressure Deficit, and Cutting Water Potential Influence Rooting of Stem Cuttings of Loblolly Pine." HortScience 39, no. 4 (July 2004): 890D—890. http://dx.doi.org/10.21273/hortsci.39.4.890d.
Full textAbstract:
Producing high quality rooted stem cuttings on a large scale requires precise management of the rooting environment. This study was conducted to investigate the effect of the rooting environment on adventitious root formation of stem cuttings of loblolly pine (Pinus taeda L.). Hardwood stem cuttings of loblolly pine were collected in Feb. 2002 from hedged stock plants and stored at 4 °C until setting in Apr. 2002. One hundred stem cuttings per plot in each of two replications received 45, 61, 73, 102, 147, or 310 mL·m-2 of mist delivered intermittently by a traveling gantry (boom) system. Mist frequency was similar for all treatments and was related inversely to relative humidity (RH) within the polyethylene covered greenhouse. Rooting tubs in each plot were filled with a substrate of fine silica sand, and substrate water potential was held constant using soil tensiometers that activated a subirrigation system. Cutting water potential was measured destructively on two cuttings per plot beginning at 0500 hr every 3 hh until 2300 hr (seven measurements) 7, 14, 21, or 28 days after setting. During rooting, leaf temperature and RH were recorded in each plot to calculate vapor pressure deficit (VPD). Cutting water potential and VPD were strongly related to mist application. Cutting water potential was also related to VPD. Rooting percentage had a linear and quadratic relationship with mean cutting water potential and VPD averaged between 1000 and 1800 HR. Eighty percent rooting occurred within a range of values for VPD. Data suggest that VPD can be used to manage the water deficit of stem cuttings of loblolly pine to increase rooting percentage. These results may be applicable to other species and to other rooting environments.
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Farmer, Robert E., Heather A. Foster, Olenka Bakowsky, Brian MacDonald, Gwenoth O'Reilly, and Ronald Reinholt. "A Vegetative Propagation System for Tamarack." Northern Journal of Applied Forestry 3, no. 3 (September 1, 1986): 91–93. http://dx.doi.org/10.1093/njaf/3.3.91.
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Abstract Greenwood cuttings from greenhouse-produced tamarack seedlings and 3- to 10-year-old tamarack wildlings transplanted from natural stands in north-western Ontario were rooted under mist in peat-vermiculite. Eighty-five percent of cuttings from seedlings rooted; treatment with indolebutyric acid increased number of roots per cutting, but not rooting percent. At 6 weeks after planting, rooting of cuttings from wildlings averaged 66%, and at 12 weeks, 91%. Twelve-week rooting percent of cuttings from individual ortets ranged from 12 to 100, but cuttings from the majority of ortets exhibited 100% rooting. Nearly all rooted cuttings survived overwintering outdoors and initiated normal shoot growth after forcing in mid-winter. The described propagation system is recommended for production of container stock for tamarack plantations. North. J. Appl. For. 3:91-93, Sept. 1986.
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Hilaire, Rolston St, and Carlos A. Fierro Berwart. "Three Mussaenda Cultivars Propagated by Stem Cuttings Exhibit Variation in Rooting in Response to Hormone and Rooting Conditions." HortTechnology 10, no. 4 (January 2000): 780–84. http://dx.doi.org/10.21273/horttech.10.4.780.
Full textAbstract:
The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.
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McNamara, Steve, William R. Graves, and Harold Pellett. "Propagation of Maackia amurensis Rupr. & Maxim, by Softwood Cuttings." Journal of Environmental Horticulture 12, no. 3 (September 1, 1994): 147–49. http://dx.doi.org/10.24266/0738-2898-12.3.147.
Full textAbstract:
Abstract Softwood terminal stem cuttings were collected at two developmental stages from twenty mature trees of Amur maackia (Maackia amurensis Rupr. and Maxim) growing in Minnesota, Maryland, and Washington DC. Cuttings were treated with either 0 or 2500 ppm (0.25%) of a potassium salt formulation of indole-3-butyric acid (K-IBA) and stuck under intermittent mist with bottom-heat. Cuttings were evaluated for rooting after 12 weeks. Cutting collection date did not affect rooting of most genotypes. Treatment with K-IBA nominally improved root rating scores and root numbers. All trees exhibited the capacity for rooting with individual rooting percentages ranging from 19% to 92%. Based upon mean visual ratings, cuttings from 14 of the 20 trees produced root systems of acceptable quality. These findings indicate that mature Maackia amurensis trees can be propagated by softwood cuttings, but refinement of the rooting protocol may be required to achieve acceptable levels of rooting for some genotypes.
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Wise, Farrell C., Frank A. Blazich, and L. Eric Hinesley. "Propagation of Abiesfraseri by softwood stem cuttings." Canadian Journal of Forest Research 15, no. 6 (December 1, 1985): 1172–76. http://dx.doi.org/10.1139/x85-190.
Full textAbstract:
Effects of collection date, crown position, and indole-3-butyric acid concentration on rooting and subsequent vegetative growth of softwood cuttings taken from 14-year-old Fraser fir (Abiesfraseri (Pursh) Poir.) Christmas trees were studied. Indole-3-butyric acid levels producing maximum rooting (approximately 50%) varied with crown position and date of severance. Abaxial bending during rooting was greatest for cuttings from the first collection (June 23). Increasing indole-3-butyric acid concentrations decreased abaxial bending of cuttings from the first two severance dates. One year after rooting, plagiotropism was still most pronounced on cuttings collected the first date as a result of active bending of the original cutting segment. Terminal shoot growth was greatest for cuttings from the first and third collections. Lateral budbreak was superior on cuttings collected on the first date. When all factors are taken into consideration, the best combination of rooting and vegetative growth was obtained from upper-crown cuttings which were collected just prior to lignification, wounded, and treated with 1500 ppm indole-3-butyric acid. After one season (one growth flush), rooted softwood cuttings were comparable to 2-year-old plants (two flushes) propagated by hardwood cuttings. The temporal separation of rooting from shoot extension may have been responsible for improving initial vegetative growth.
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Guasso, Leonardo Zucuni, Francisco Antonello Marodin, Taís Altmann, Samar Velho da Silveira, and Paulo Vitor Dutra de Souza. "Propagation of ‘Bruno’ kiwifruit: influence of cutting collection time and indolebutyric acid." Semina: Ciências Agrárias 42, no. 4 (May 20, 2021): 2203–16. http://dx.doi.org/10.5433/1679-0359.2021v42n4p2203.
Full textAbstract:
In Brazil, the propagation of kiwifruit (Actinidia deliciosa) is achieved through seed-derived rootstocks, which results in low uniformity. An alternative to address this problem is the cuttings technique, a simple and fast method that generates individuals identical to the parent plant, maintaining the agronomic traits. This study was conducted to evaluate the effects of cutting collection time and indolebutyric acid (IBA) on the rooting of ‘Bruno’ kiwifruit cuttings. The cuttings were collected in the months of May, August and December 2016 and March 2017 and treated with 0, 1000, 2000 and 4000 mg L-1 IBA, as a hydroalcoholic solution, for seven seconds. After 90 days, the percentages of rooted and dead cuttings and cuttings that produced shoots; leaf retention; average length of the three largest roots; average number of primary roots per cutting; and reserve contents before and after rooting were evaluated. The treatments with IBA influenced the rooting of cuttings collected in May, which showed 36.07% rooting at the calculated IBA concentration of 2,320 mg L-1, and in December, which exhibited 57.59% rooting at the concentration of 4000 mg L-1. In the present experimental conditions, it is recommended to root cuttings of ‘Bruno’ kiwifruit collected in December with the application of 4000 mg L-1 IBA.
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Exadaktylou, Efstathia, Thomas Thomidis, Brian Grout, George Zakynthinos, and Constantinos Tsipouridis. "Methods to Improve the Rooting of Hardwood Cuttings of the ‘Gisela 5’ Cherry Rootstock." HortTechnology 19, no. 2 (January 2009): 254–59. http://dx.doi.org/10.21273/hortsci.19.2.254.
Full textAbstract:
The effect of external factors on the rooting of hardwood cuttings of the ‘Gisela 5’ cherry rootstock (Prunus cerasus × Prunus canescens) was studied. Experiments were conducted with cuttings of different lengths (15, 20, 25, and 30 cm) and diameters (6 to 8, 9 to 11, and 12 to 14 mm) and with different indole-3-butyric acid (IBA) concentrations (0, 1, 2, 3, 4, and 6 g·L−1). Significantly improved rooting was achieved using cuttings 20 cm long and 9 to 11 mm in diameter collected in November or March from the upper part of the parent shoot and subsequently quick-dipped in 1 g·L−1 IBA solution. Cuttings placed vertically in perlite:peat (1:1) or perlite with bottom heat gave the best rooting percentage. No effect on callusing or rooting of cuttings was found after incubation of cutting in suspensions of Agrobacterium rubi. If the optimal combination of factors is selected, ‘Gisela 5’ can be propagated from hardwood cuttings with a rooting efficiency of ≈50%.
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DUARTE, Manoela Mendes, Maria Cecília MIRESKI, Afonso OLISZESKI, Ivar WENDLING, and Carlos André STUEPP. "Rooting of yerba mate cuttings with different lengths." Revista Eletrônica Científica da UERGS 5, no. 1 (April 16, 2019): 6–12. http://dx.doi.org/10.21674/2448-0479.51.6-12.
Full textAbstract:
Due to its economic, social, and ecological potential, Ilex paraguariensis A. St. Hil is considered one of the most important native forestry species in southern Brazil, needing high production of quality plants to supply consumption. Therefore, vegetative propagation is a key technique since it enables plants production with higher standard and genetic quality. Several studies have focused on defining appropriate methods for rooting yerba mate cuttings, even though the overall rooting indexes are not yet considered appropriate. Thus, the objective of this study was to evaluate the rooting, root and aerial vigor of yerba mate cuttings and determine the best cuttings length for formation of clonal plants. Twenty parent trees were pruned and after five months epimoric sprouts were collected to prepare stem cuttings with lengths of 2 ± 0.2 cm, 4 ± 0.2 cm, 6 ± 0.2 cm, 8 ± 0.2 cm, 10 ± 0.2 cm and 12 ± 0.2 cm and diameter of 0.5 ± 0.2 cm. We planted cuttings in plastic boxes filled with substrate based on carbonized yerba mate matchsticks and vermiculite. After 120 days we evaluated the percentage of rooting, number of roots per cutting, average length of the 3 largest roots per cutting, cuttings with callus, cuttings with roots and callus, mortality, sprouts emission, and leaf maintenance. Our results show that rooting and vigor of roots in yerba mate stem cuttings are influenced by cuttings length, being 10 cm cuttings the most efficient for clonal propagation of the species.
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Mello, Simone da Costa, Jéssika Angelotti-Mendonça, Lucas Baiochi Riboldi, Luigi Tancredi Campo Dall’Orto, and Eduardo Suguino. "Impact of Indole-3-Butyric Acid on Adventitious Root Development from Cuttings of Tea." HortTechnology 26, no. 5 (October 2016): 599–603. http://dx.doi.org/10.21273/horttech03378-16.
Full textAbstract:
The objective of this study was to investigate the effects of the application of indole-3-butyric acid (IBA) at concentrations of 0, 30, 60, and 90 mg·L−1, for 24 hours, on rooting of softwood and semihardwood cuttings of tea (Camellia sinensis var. sinensis ‘Yabukita’ and C. sinensis var. assamica ‘IAC-259’) collected in winter and summer. In the summer, IBA increased root percentage of softwood cuttings from ‘Yabukita’ compared with the control. However, the rooting of semihardwood cuttings was unaffected by this growth regulator. In winter, application of 90 mg·L−1 IBA increased the rooting regardless of the type of ‘Yabukita’ cuttings. In addition, in ‘IAC-259’, there was an increase in dry weight, number of roots (NOR), and rooting percentage of softwood cuttings collected in summer with application of 90 mg·L−1 IBA compared with control. In contrast, during the same period of the year, the semihardwood cuttings of ‘IAC-259’ were unaffected by the IBA. In winter, the percentage of cutting survival, rooting, the number, and length of roots were unaffected by IBA in ‘IAC-259’. Overall, we would recommend the use of exogenous IBA for rooting of cuttings collected in the summer or winter.
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Griffin, Jason J., Frank A. Blazich, and Thomas G. Ranney. "Propagation of Thuja × `Green Giant' by Stem Cuttings: Effects of Growth Stage, Type of Cutting, and IBA Treatment." HortScience 33, no. 3 (June 1998): 504a—504. http://dx.doi.org/10.21273/hortsci.33.3.504a.
Full textAbstract:
Stem cuttings of Thuja L. × `Green Giant', consisting of 22-cm terminals or 20-cm laterals, were collected on three dates associated with specific growth stages (softwood, semi-hardwood, and hardwood), and treated with indolebutyric acid (IBA) in 50% isopropanol ranging from 0 to 9000 ppm and placed under intermittent mist. Regardless of cutting type or auxin treatment, cuttings rooted in high percentages at each growth stage. Overall rooting was highest for hardwood cuttings (96%) followed by semi-hardwood (86%), and softwood (85%) cuttings. Semi-hardwood cuttings were the only cuttings in which rooting was affected by IBA or cutting type with the greatest rooting (94%) attained using lateral cuttings treated with 6000 or 9000 ppm IBA. Although an interaction occurred between IBA treatment and cutting type for mean root length of hardwood cuttings, only mean root length of semi-hardwood cuttings was influenced by IBA concentration alone with the greatest length (25 mm) achieved with 6000 ppm IBA. An interaction was noted for root count between cutting type and IBA concentrations for softwood cuttings, whereas IBA and cutting type affected independently root count on semi-hardwood cuttings. Treatment with 9000 ppm IBA resulted in 18 roots per rooted cutting whereas lateral cuttings responded with a mean of 16 roots over all IBA concentrations. Root count on hardwood cuttings was affected by IBA treatment only, with 9000 ppm being optimum (14 roots per rooted cutting).
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Pan, Ting, Xue-lian Chen, Yan-ping Hao, Chun-wu Jiang, Song Wang, Jin-shan Wang, Qiang Wei, et al. "Optimization of factors affecting the rooting of pine wilt disease resistant Masson pine (Pinus massoniana) stem cuttings." PLOS ONE 16, no. 9 (September 10, 2021): e0251937. http://dx.doi.org/10.1371/journal.pone.0251937.
Full textAbstract:
Pine wilt disease (PWD) is a devastating disease affecting trees belonging to the genus Pinus. To control the spread of PWD in the Masson pine forest in China, PWD resistant Masson pine clones have been selected by the Anhui Academy of Forestry. However, because Masson pine is a difficult-to-root species, producing seedlings is challenging, especially from trees older than 5 years of age, which impedes the application of PWD resistant clones. In this study, we investigated the factors affecting rooting of PWD resistant clones and established a cheap, reliable, and simple method that promotes rooting. We tested the effects of three management methods, four substrates, two cutting materials, two cutting treatments, and three collection times on the rooting of cuttings obtained from 9-year-old PWD resistant clones. Rooting was observed only in stem cuttings treated with the full-light automatic spray management method. Additionally, stem cuttings showed a significantly higher rooting rate and root quality than needles cuttings. Compared with other substrates, stem cuttings planted in perlite produced the longest adventitious root and the highest total root length and lateral root number. Moreover, stem cuttings of PWD resistant clones collected in May showed a significantly higher rooting rate and root quality than those collected in June and July. Moreover, stem cuttings prepared with a horizontal cut while retaining the needles showed significantly higher rooting rate and root quality than those prepared with a diagonal cut while partly removing the needles. This study promotes the reproduction of seedlings of PWD-resistant Masson pine clones which helps control the spread of PWD, meanwhile, provides a technical reference for the propagation of mature pine trees via cuttings.
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Caplan, Deron, Jonathan Stemeroff, Mike Dixon, and Youbin Zheng. "Vegetative propagation of cannabis by stem cuttings: effects of leaf number, cutting position, rooting hormone, and leaf tip removal." Canadian Journal of Plant Science 98, no. 5 (October 1, 2018): 1126–32. http://dx.doi.org/10.1139/cjps-2018-0038.
Full textAbstract:
This study evaluated the influence of several factors and their interactive effects on the propagation success of stem cuttings of cannabis (Cannabis sativa L.). Factors included (i) leaf number (two or three), (ii) leaf tip removal (one-third of leaf tips removed), (iii) basal/apical position of stem cutting on the stock plant, and (iv) rooting hormone [0.2% indole-3-butyric (IBA) acid gel or 0.2% willow (Salix alba L.) extract gel]. Cuttings were placed in a growth chamber for twelve days and then assessed on their rooting success rate and root quality using a relative root quality scale. The IBA gel delivered a 2.1× higher rooting success rate and 1.6× higher root quality than the willow extract. Removing leaf tips reduced rooting success rate from 71% to 53% without influencing root quality. Cuttings with three leaves had 15% higher root quality compared with those with two, but leaf number did not influence rooting success rate. Position of cutting had little effect on rooting success or quality. To achieve maximum rooting success and root quality, cuttings from either apical or basal positions should have at least three fully expanded uncut leaves and the tested IBA rooting hormone is preferred to the willow-based product.
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Maynard, Brian K., and Nina L. Bassuk. "STOCKPLANT ETIOLATION, SHADING, AND STEM BANDING EFFECTS ON THE AUXIN DOSE-RESPONSE OF ROOTING IN SOFTWOOD STEM CUTTINGS OF CARPINUS BETULUS `FASTIGIATA'." HortScience 25, no. 9 (September 1990): 1126a—1126. http://dx.doi.org/10.21273/hortsci.25.9.1126a.
Full textAbstract:
Three experiments were undertaken to examine the effect of stockplant etiolation, shading, and stem banding, prior to cutting propagation, on the auxin dose-response of rooting in stem cuttings of Carpinus betulus `fastigiata'. A 2 × 2 factorial of etiolation and banding utilized stockplants forced in a greenhouse, etiolated for 1 week and banded with Velcro™ for 1 month. In a separate study shading was applied up the time of harvesting cuttings. IBA was applied to cuttings as an aqueous ethanol quick dip in concentrations ranging from 0 to 80 mM. Rooting percentage and number were best described, up to a peak response, by a linear function proportional to the logarithm of applied IBA. The inhibition of rooting by supra-optimal IBA was directly proportional to IBA concentration. Cuttings prepared from shoots which had been etiolated or banded rooted better at low IBA and at their respective optimal IBA levels. Cuttings from shoots receiving both etiolation and banding yielded higher rooting percentages and more roots per rooted cutting on average. Etiolation and banding served to increase both initial and maximum rooting capacities, and to reduce the sensitivity of cuttings to supraoptimal auxin-induced inhibition of adventitious root initiation. The auxin dose-response interacted with shading to yield the best rooting at 95% shade and 3.7 mM IBA.
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Sharma, Jyotsna, Gary Knox, and Maria Ishida. "(169) Root Formation on Stem Cuttings of Yellow-flowered Cultivars of Magnolia Is Influenced by Time-after-budbreak and IBA." HortScience 40, no. 4 (July 2005): 1001A—1001. http://dx.doi.org/10.21273/hortsci.40.4.1001a.
Full textAbstract:
We propagatedsixyellow-flowered cultivars of Magnolia vegetatively by applying 0, 8, 16, or 30 g·kg-1 indole-3-butyric acid (IBA) in talc to bases of terminal stem cuttings collected 5, 7, 9, or 11 weeks after budbreak. Mean rooting percentage increased from 12% (in the absence of IBA) to 34% (after applying 30 g·kg-1 IBA). Rooting percentage also increased with increasing basal caliper (r2 = 0.25; P< 0.0001) of a cutting. For each collection date, more cuttings of `Ivory Chalice' and `Yellow Lantern' developed roots than did other cultivars. When data were analyzed separately for selected cultivars, 63% rooting was observed among cuttings of `Ivory Chalice' collected 7 weeks after budbreak. Rooting percentage was higher (22%) among cuttings of `Hot Flash' collected 5 or 7 weeks after budbreak in comparison to later collection dates, but harvest date did not influence rooting, which ranged from 44% to 59%, among cuttings of `Yellow Lantern'. Collection of stem cuttings early in the growing season (5 weeks after budbreak) was beneficial (31% rooting) for inducing root formation among cuttings of `Golden Sun'. We conclude that `Ivory Chalice' and `Yellow Lantern' are promising choices for growers interested in clonal propagation of yellow-flowered cultivars of Magnolia. To maximize rooting, terminal cuttings should be collected within 5 to 11 weeks after budbreak and should be treated with 16 or 30 g·kg-1 IBA in talc. Early collection dates improved rooting frequencies among cuttings of other cultivars but these, particularly `Butterflies', remain variably recalcitrant and merit further study.
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Budiarto, K., Y. Sulyo, E. Dwi S.N., and R. H. M. Maaswinkel. "EFFECTS OF TYPES OF MEDIA AND NPK FERTILIZER ON THE ROOTING CAPACITY OF CHRYSANTHEMUM CUTTINGS." Indonesian Journal of Agricultural Science 7, no. 2 (October 25, 2016): 67. http://dx.doi.org/10.21082/ijas.v7n2.2006.67-70.
Full textAbstract:
Physiological status of chrysanthemum cuttings during rooting process highly influences plant growth and flower production. Amongst other aspects, rooting capacity of the cuttings is the most important. Rooting capacity is influenced by environmental conditions such as growing medium and nutrition. The study was aimed to analyze the effects of different types of media, fertilizer concentration, and frequency of fertilizer application on the rooting capacity of chrysanthemum cuttings. The experiment was conducted at the Segunung Experimental Station of the Indonesian Ornamental Crops Research Institute from February to May 2005. The treatments consisted of four different rooting media, i.e. carbonized rice husk, coco peat, perlite, and mixture of perlite and vermiculite (1:1 v/v); two concentrations of NPK (25:7:7) fertilizers, i.e. 1.75 and 2.5 g 1-l; and four frequencies of fertilizer application, i.e. every 1 day, 2 days, 3 days, and 4 days. The experiment was designed in a completely randomized block with four replications. Parameters observed were rooting capacity and cutting performances such as cutting height, cutting diameter, number of leaves, number of visible roots, and root length at 16 days after planting. The results showed that chrysanthemum cuttings rooted in carbonized rice husk had better performance than those rooted in coco peat, vermiculite, and mixture of perlite + vermiculite. Higher concentration of fertilizer solution up to 2.5 g l-1 also gave significant effect on the root formation and further root growth of cuttings. However, the rooting capacity of cuttings was not affected by the frequency of fertilizer application. This study implied that carbonized rice husk growing medium supplemented with NPK (2.5 g l-l) every 4 days during rooting period is the choice for traditional chrysanthemum growers to increase the perform-ance and quality of the cut flowers.
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Budiarto, K., Y. Sulyo, E. Dwi S.N., and R. H. M. Maaswinkel. "EFFECTS OF TYPES OF MEDIA AND NPK FERTILIZER ON THE ROOTING CAPACITY OF CHRYSANTHEMUM CUTTINGS." Indonesian Journal of Agricultural Science 7, no. 2 (October 25, 2016): 67. http://dx.doi.org/10.21082/ijas.v7n2.2006.p67-70.
Full textAbstract:
Physiological status of chrysanthemum cuttings during rooting process highly influences plant growth and flower production. Amongst other aspects, rooting capacity of the cuttings is the most important. Rooting capacity is influenced by environmental conditions such as growing medium and nutrition. The study was aimed to analyze the effects of different types of media, fertilizer concentration, and frequency of fertilizer application on the rooting capacity of chrysanthemum cuttings. The experiment was conducted at the Segunung Experimental Station of the Indonesian Ornamental Crops Research Institute from February to May 2005. The treatments consisted of four different rooting media, i.e. carbonized rice husk, coco peat, perlite, and mixture of perlite and vermiculite (1:1 v/v); two concentrations of NPK (25:7:7) fertilizers, i.e. 1.75 and 2.5 g 1-l; and four frequencies of fertilizer application, i.e. every 1 day, 2 days, 3 days, and 4 days. The experiment was designed in a completely randomized block with four replications. Parameters observed were rooting capacity and cutting performances such as cutting height, cutting diameter, number of leaves, number of visible roots, and root length at 16 days after planting. The results showed that chrysanthemum cuttings rooted in carbonized rice husk had better performance than those rooted in coco peat, vermiculite, and mixture of perlite + vermiculite. Higher concentration of fertilizer solution up to 2.5 g l-1 also gave significant effect on the root formation and further root growth of cuttings. However, the rooting capacity of cuttings was not affected by the frequency of fertilizer application. This study implied that carbonized rice husk growing medium supplemented with NPK (2.5 g l-l) every 4 days during rooting period is the choice for traditional chrysanthemum growers to increase the perform-ance and quality of the cut flowers.
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Górecki, Ryszard S. "Physiological changes in M 26 and M 106 softwood apple (Malus sp. Mill.) cuttings during their rooting." Acta Agrobotanica 33, no. 1 (2013): 109–19. http://dx.doi.org/10.5586/aa.1980.010.
Full textAbstract:
The levels of rooting cofactors, monohydroxyphenols, o-dihydroxyphenols, total phenols and protein, as well as the activity of peroxidase and polyphenol oxydase were compared in the leaves and stems of untreated vs IBA-treated cuttings. The cuttings of M 26 and M 106 clone were analysed on days: 0, 16 and 24 of rooting. Marked differences were found between the clones and treatments compared in levels of the substances analysed but these were not correlated with the rooting ability of cuttings. Only low monophenols' content in the IBA-treated M 106 cutting stems was correlated with the best rooting obtained.