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Relevant bibliographies by topics / RP-HPLC Method

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Academic literature on the topic 'RP-HPLC Method'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "RP-HPLC Method"

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Jinal, Harshadkumar Rathod *. Jaymin G. Patel Bhumi R. Patel. "ESTIMATION OF CLOTRIMAZOLE, CLINDAMYCIN PHOSPHATE AND TINIDAZOLE BY VARIOUS ANALYTICAL METHOD: REVIEW ARTICLE." Journal of Pharma Research 8, no. 4 (2019): 160–68. https://doi.org/10.5281/zenodo.2647870.

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<strong><em>ABSTRACT</em></strong> <strong><em>A</em></strong><em>nalytical method development and its validation is an important aspect in drug discovery process. Development of analytical method producing accurate and precise data is necessary to ensure the quality and safety of the drugs. At present, the most common analytical method employed for estimation of drugs is Reverse Phase High Pressure Liquid Chromatography (RP-HPLC) because of its high sensitivity, accuracy and speed. Different types of analytical methods are available for estimation of Clindamycin Phosphate, Clotrimazole and Ti

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Akshata, M. Girase, M. Mahale Bhupendra, R.Dhankani Amitkumar, and p. pawar Sunil. "Review On: RP-HPLC." Journal of Advances in Experimental Therapeutics and Neurotherapeutics 2, no. 2 (2024): 21–27. https://doi.org/10.5281/zenodo.14257869.

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<em>Pharmaceutical analysis plays a vital role in determining the qualitative and quantitative composition of materials, essential for understanding sample characteristics. Analytical chemistry rifts into two branches qualitative, which classifies components, and quantitative, which measures them. In recent years, high-performance liquid chromatography (HPLC) has gained prominence for its ability to separate, identify, and quantify various components in biological fluids, pharmaceutical formulations, and drug samples. Notably, reversed-phase chromatography (RP-HPLC) is widely used due to its e

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KHANDARE, BALU, Atish C. Musle, Sanket S. Arole, and Pravin V. Popalghat. "Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC Method." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 608–11. http://dx.doi.org/10.22270/jddt.v9i4-a.3527.

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RP-HPLC is fast, simple, sensitive, precise, and reproducible (liquid chromatography) method, developed and validated to analyseolmutinib bulk dosage form. Using C-18 HPLC column separation was carried out. This was maintained at ambient temperature. During separation mobile phase consist of methanol (100 v/v) was delivered at a rate of 1mL/min. Using UV detector analysis was carried out at the wavelength 267.68 nm. RP-HPLC method was validated by using various parameters like, precision, limit of quantitation (LOQ), linearity and robustness. The RP-HPLC method was found to be linear over the

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Madhuri, R. Shirsath Sonali A. Waghmare And Pradyumna P. Ige. "A Review On Recent Advances In Development Of RP-HPLC Method." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2674–82. https://doi.org/10.5281/zenodo.13242715.

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The development and validation of analytical methods play crucial roles in drug discovery, drug development, and the production of different pharmaceutical formulations. A straightforward, accurate, exact, and targeted RP-HPLC approach was created to determine the drug in pharmaceuticals. Because of its greater selectivity and flexibility for hydrophobic substances, reverse-phase liquid chromatography (RP-HPLC) is the separation method of choice for high-performance liquid chromatography (HPLC). The development of RP HPLC methods has advanced significantly in recent years, with new stationary

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Parmar, Raghuveersinh, Dr Priyanka Patil, Dr Chainesh Shah, and Dr Umesh Upadhyay. "A Review Article on Method Development and Validation of Verapamil by RP-HPLC Method." International Journal of Pharmaceutical Research and Applications 09, no. 05 (2024): 977–88. https://doi.org/10.35629/4494-0905977988.

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High performance liquid chromatography, one of the most powerful analytical techniques utilized in the separation, identification, and quantification of complex mixtures. Reverse-phase or RP-HPLC is commonly practiced in high performance liquid chromatography because this technique has the advantages of versatility and appropriateness for more hydrophobic compounds. Fundamentals and Practices of Reversed-Phase HPLC: Part II -- RPHPLC column configurations. It consists of the concept of separation, types of stationary and mobile phases with the variables concerned with the separation. This revi

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Kumar, Deepak, Amrendra Kumar, Vinay Kumar, et al. "A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications." Journal for Research in Applied Sciences and Biotechnology 2, no. 2 (2023): 53–60. http://dx.doi.org/10.55544/jrasb.2.2.9.

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The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highl

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Vinayak, A. katekar, P. Kothari Prafful, S. Kawarkhe Swapnil, P. Surung Manish, and B. Akotkar Vaishnavi. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. https://doi.org/10.5281/zenodo.8265469.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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Vinayak A. katekar, Prafful P. Kothari, Swapnil S. Kawarkhe, Manish P. Surung, and Vaishnavi B. Akotkar. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. http://dx.doi.org/10.30574/gscbps.2023.24.1.0233.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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Sagar, N. Katke*1 Santosh P. Kumbhar2 Vinod D. Usnale3 Siddhant M. Sawant4. "The Method Development And Validation Of A High-Performance Liquid Chromatographic Method For Azilsartan Analysis." International Journal in Pharmaceutical Sciences 2, no. 5 (2024): 1152–61. https://doi.org/10.5281/zenodo.11240815.

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The goal of the current study is to develop an RP-HPLC-based analytical method for determining the dosage of Azilsartan in bulk and tablet form that is fast, precise, sensitive, selective, and repeatable. Create a novel HPLC technique for Azilsartan estimation, and validate it in compliance with ICH guidelines. In order to make use of the accepted methodology for Azilsartan estimation in pharmaceutical formulations, an RP-HPLC method was utilized to create and validate a stability indicating method. Using an Inertsil-ODS C18 (250×4.6mm, 5µm) column and a 90:10 v/v methanol: acetoni

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Gulabrao Bhamare, Vaibhav, and Ravindra K. Kamble. "DETERMINATION OF ETODOLAC BY RP-HPLC METHOD." International Research Journal Of Pharmacy 11, no. 9 (2020): 25–30. http://dx.doi.org/10.7897/2230-8407.110980.

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Dissertations / Theses on the topic "RP-HPLC Method"

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Jakob, Ronit Julia [Verfasser]. "Entwicklung von chiralen- sowie RP-HPLC-Methoden in Verbindung mit hochauflösender MS und deren Anwendung zur Analyse sekundärer organischer Aerosole in der Atmosphäre / Ronit Julia Jakob." Mainz : Universitätsbibliothek Mainz, 2015. http://d-nb.info/1079820493/34.

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Maldaner, Liane. "Fases estacionarias de poli(metil-3,3,3-trifluorpropilsiloxano) imobilizadas sobre suportes de silica para cromatografia liquida de alta eficiencia em fase reversa = preparo e caracterização." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250536.

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Orientador: Isabel Cristina Sales Fontes Jardim<br>Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica<br>Made available in DSpace on 2018-08-15T06:33:14Z (GMT). No. of bitstreams: 1 Maldaner_Liane_D.pdf: 4323260 bytes, checksum: 773bada382fd206c910361612918f566 (MD5) Previous issue date: 2010<br>Resumo: Novas fases estacionárias (FE) fluoradas foram preparadas a partir da imobilização do poli(metil-3,3,3-trifluorpropilsiloxano) (PMTFS) em partículas de sílica Kromasil de 5 mm, por tratamento termico (TT) ou por radiação micro-ondas (RM). As condições de imobilização o

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Van, Wyk Pieter-Hugo. "Novel methods of chemical speciation of Pt(IV/II) complexes in acid halide-rich solutions by ion-pair RP-HPLC coupled to ICP-OES/MS in conjunction with 195Pt NMR." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/80099.

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Thesis (PhD)--Stellenbosch University, 2013.<br>ENGLISH ABSTRACT: In this work a robust reversed phase ion-pairing high performance liquid chromatographic (RP-HPLC) method has been developed for the separation, characterization and quantification of all possible [PtCl6-nBrn]2- (n = 0 – 6) and [PtCl4-nBrn]2- (n = 0 – 4) complex anions using UV-Vis detection. High resolution 195Pt NMR of more concentrated PtII/IV solutions served to validate the relevant species assignments, particularly those of the stereoisomer species, cis- and trans- [PtCl4Br2]2-, [PtCl2Br4]2- and mer- and fac-[PtCl3Br3

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(5929574), Yuan Chen. "PHOTOLYTIC LABELING TO PROBE PEPTIDE-MATRIX INTERACTIONS IN LYOPHILIZED SOLIDS." Thesis, 2020.

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<p>Therapeutic proteins are often lyophilized with excipients such as sucrose or trehalose to protect them during manufacturing and achieve a longer shelf-life. Formulation design for therapeutic proteins has been a trial-and-error process, and the mechanisms responsible for the stabilizing effects of excipients are not fully understood. Two proposed theories have been widely accepted: the water replacement theory and the vitrification theory.<sup>1,2</sup>The water replacement theory suggests that excipients stabilize protein molecules in the solid state by forming hydrogen bonds that “replac

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Books on the topic "RP-HPLC Method"

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Rp-Hplc Stability Indicating Assay and Simultaneous Estimation Method for the Combination of Glecapravir and Pibrentasvir in Tablet Dosage Form. Independently Published, 2020.

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Book chapters on the topic "RP-HPLC Method"

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Roja, Aavula, Peram Uma Maheshwari, Ramapuram Munemma, and Konda Swathi. "Method Development and Validation of Gallic Acid in Liquid Dosage Form by Using RP-HPLC Method." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-31986-0_51.

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Petruczynik, Anna, and Monika Waksmundzka-Hajnos. "Analysis of Basic Psychotropic Drugs in Biological Fluids and Tissues by RP-HPLC Method." In Handbook of Bioanalytics. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-95660-8_9.

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Petruczynik, Anna, and Monika Waksmundzka-Hajnos. "Analysis of Basic Psychotropic Drugs in Biological Fluids and Tissues by RP-HPLC Method." In Handbook of Bioanalytics. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-63957-0_9-1.

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Quaratino, C. P., E. Messina, P. Bertuglia, et al. "A RP-HPLC Method for the Measurement of Guanine, Other Purine Bases and Nucleosides." In Advances in Experimental Medicine and Biology. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-7703-4_9.

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Dhiraj, Kumar, and Himansu Bhusan Samal. "Development of stability indicating RP-HPLC method for simultaneous estimation of sacubitril and valsartan in pure and pharmaceutical dosage form." In Advancement in Animal Handling and Generative AI for Pre-clinical Studies. CRC Press, 2025. https://doi.org/10.1201/9781003672869-20.

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Welinder, Karen G. "Proteolytic Cleavage in RP-HPLC Solvents." In Methods in Protein Sequence Analysis · 1986. Humana Press, 1987. http://dx.doi.org/10.1007/978-1-59259-480-1_25.

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Cosme, Fernanda, Juliana Milheiro, Leonor C. Ferreira, Luís Filipe-Ribeiro, and Fernando M. Nunes. "Methods to Determine Biogenic Amines in Wine by RP-HPLC." In Basic Protocols in Enology and Winemaking. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3088-4_14.

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Xu, Jie, and Jeffrey D. Clogston. "Ion Quantitation in Liposomal Products Using RP-HPLC with Charged Aerosol Detection." In Methods in Molecular Biology. Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3786-9_7.

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Van den Bergh, Gert, and Lutgarde Arckens. "Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg." In Methods in Molecular Biology™. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-064-9_13.

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Zhang, Jiang, Kevin A. Lanham, Warren Heideman, Richard E. Peterson, and Lingjun Li. "Characterization of Zebrafish Cardiac Proteome Using Online pH Gradient SCX–RP HPLC–MS/MS Platform." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-386-2_10.

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Conference papers on the topic "RP-HPLC Method"

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Kumar, Praveen, Meenu Chaudhary, Vaibhav Rathi, Bhupendra Singh, Manish Vyas, and Geetanjali Saini. "Validated analytical method development for drug residue (Clonazepam) in pharmaceutical industries by RP-HPLC." In THE FOURTH SCIENTIFIC CONFERENCE FOR ELECTRICAL ENGINEERING TECHNIQUES RESEARCH (EETR2022). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0162987.

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Bor, Marisabel, Dhaimarys Villalba, Maria Sánchez, Barbara Delgado, and Glen Zea. "Validation of method for RP-HPLC for the determination of Etoricoxib in coated tablets." In 7th International Electronic Conference on Medicinal Chemistry. MDPI, 2021. http://dx.doi.org/10.3390/ecmc2021-11443.

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Sulistyowati, Etty, Bekti Nugraheni, and Aries Koes Sundoro. "Mobile phase optimization of RP-HPLC method for quantification of quercetin in Moringa leaf fraction." In 3RD INTERNATIONAL CONFERENCE OF BIO-BASED ECONOMY FOR APPLICATION AND UTILITY. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0120358.

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Zhou, Ran, Ming Chang, Sijie Liu, Fei Wang, and Hongkun Yue. "Rapid determination of the content of dihydroergotoxine mesilate for injection by an RP-HPLC method." In International conference on Human Health and Medical Engineering. WIT Press, 2014. http://dx.doi.org/10.2495/hhme130451.

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Al-Samarrai, Saif Dahham Hameedi, and Eman Thiab Al-Samarraie. "Development and validation of RP-HPLC method analysis for COVID-19 antiviral drug Samavir tablet." In 1ST SAMARRA INTERNATIONAL CONFERENCE FOR PURE AND APPLIED SCIENCES (SICPS2021): SICPS2021. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0120911.

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Chang, Ming, Ran Zhou, Sijie Liu, Fei Wang, and Hongwei Yu. "Development of an RP-HPLC method for evaluation of the content of cefotiam hydrochloride for injection." In International conference on Human Health and Medical Engineering. WIT Press, 2014. http://dx.doi.org/10.2495/hhme130441.

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Joy, Muthipeedika Nibin, Rishikesan Rathnasamy, Ranjith P. Karuvalam, et al. "An efficient RP-HPLC method for UV/mass triggered purification of bis-thiazol-2-amine derivatives." In ACTUAL PROBLEMS OF ORGANIC CHEMISTRY AND BIOTECHNOLOGY (OCBT2020): Proceedings of the International Scientific Conference. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0069241.

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Martono, Yohanes, Sugeng Riyanto, Abdul Rohman, and Sudibyo Martono. "Improvement method of fast and isocratic RP-HPLC analysis of major diterpene glycoside from Stevia rebaudiana leaves." In PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2015. Author(s), 2016. http://dx.doi.org/10.1063/1.4958509.

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Manikandan, K., K. S. Lakshmi, and Aswitha Pai. "QbD approach in RP-HPLC method development for the assay of benzocaine and diclofenac in dosage forms." In THE 11TH NATIONAL CONFERENCE ON MATHEMATICAL TECHNIQUES AND APPLICATIONS. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5112268.

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Ruttanakorn, Kanong, Apipawat Detpan, Mongkol Thongkham, et al. "Development of a Validated RP-HPLC Method for Quantification of Four Bioactive Phenolic Compounds from Mulberry Leaf Infusion." In 5th International Conference and Exhibition on Pharmaceutical Sciences and Technology 2022. Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-bey5s1.

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The aim of this work was to develop and validate RP-HPLC method for quantification of 4 major polyphenolic compounds of mulberry leaf infusion. The mulberry leaf samples were extracted by simulation of tea infusion beverage preparation. HPLC-DAD analysis combined with column C-18 150 mm x 4.6 mm, 2.7 μm was used to determine bioactive polyphenols such as chlorogenic acid, caffeic acid, rutin, and quercetin. The optimal conditions involved the flow rate of mobile phase at 0.3 ml/min with gradient elution of 0.1% formic acid in water and methanol, column temperature at 35 °C, 2 μl injection volu

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