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Journal articles on the topic 'RP-HPLC Method'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Jinal, Harshadkumar Rathod *. Jaymin G. Patel Bhumi R. Patel. "ESTIMATION OF CLOTRIMAZOLE, CLINDAMYCIN PHOSPHATE AND TINIDAZOLE BY VARIOUS ANALYTICAL METHOD: REVIEW ARTICLE." Journal of Pharma Research 8, no. 4 (2019): 160–68. https://doi.org/10.5281/zenodo.2647870.

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<strong><em>ABSTRACT</em></strong> <strong><em>A</em></strong><em>nalytical method development and its validation is an important aspect in drug discovery process. Development of analytical method producing accurate and precise data is necessary to ensure the quality and safety of the drugs. At present, the most common analytical method employed for estimation of drugs is Reverse Phase High Pressure Liquid Chromatography (RP-HPLC) because of its high sensitivity, accuracy and speed. Different types of analytical methods are available for estimation of Clindamycin Phosphate, Clotrimazole and Ti

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2

Akshata, M. Girase, M. Mahale Bhupendra, R.Dhankani Amitkumar, and p. pawar Sunil. "Review On: RP-HPLC." Journal of Advances in Experimental Therapeutics and Neurotherapeutics 2, no. 2 (2024): 21–27. https://doi.org/10.5281/zenodo.14257869.

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<em>Pharmaceutical analysis plays a vital role in determining the qualitative and quantitative composition of materials, essential for understanding sample characteristics. Analytical chemistry rifts into two branches qualitative, which classifies components, and quantitative, which measures them. In recent years, high-performance liquid chromatography (HPLC) has gained prominence for its ability to separate, identify, and quantify various components in biological fluids, pharmaceutical formulations, and drug samples. Notably, reversed-phase chromatography (RP-HPLC) is widely used due to its e

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3

KHANDARE, BALU, Atish C. Musle, Sanket S. Arole, and Pravin V. Popalghat. "Analytical method development and validation of olmutinib bulk drug as per ICH Q2 guidelines by using RP-HPLC Method." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 608–11. http://dx.doi.org/10.22270/jddt.v9i4-a.3527.

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RP-HPLC is fast, simple, sensitive, precise, and reproducible (liquid chromatography) method, developed and validated to analyseolmutinib bulk dosage form. Using C-18 HPLC column separation was carried out. This was maintained at ambient temperature. During separation mobile phase consist of methanol (100 v/v) was delivered at a rate of 1mL/min. Using UV detector analysis was carried out at the wavelength 267.68 nm. RP-HPLC method was validated by using various parameters like, precision, limit of quantitation (LOQ), linearity and robustness. The RP-HPLC method was found to be linear over the

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4

Madhuri, R. Shirsath Sonali A. Waghmare And Pradyumna P. Ige. "A Review On Recent Advances In Development Of RP-HPLC Method." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 2674–82. https://doi.org/10.5281/zenodo.13242715.

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The development and validation of analytical methods play crucial roles in drug discovery, drug development, and the production of different pharmaceutical formulations. A straightforward, accurate, exact, and targeted RP-HPLC approach was created to determine the drug in pharmaceuticals. Because of its greater selectivity and flexibility for hydrophobic substances, reverse-phase liquid chromatography (RP-HPLC) is the separation method of choice for high-performance liquid chromatography (HPLC). The development of RP HPLC methods has advanced significantly in recent years, with new stationary

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Parmar, Raghuveersinh, Dr Priyanka Patil, Dr Chainesh Shah, and Dr Umesh Upadhyay. "A Review Article on Method Development and Validation of Verapamil by RP-HPLC Method." International Journal of Pharmaceutical Research and Applications 09, no. 05 (2024): 977–88. https://doi.org/10.35629/4494-0905977988.

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High performance liquid chromatography, one of the most powerful analytical techniques utilized in the separation, identification, and quantification of complex mixtures. Reverse-phase or RP-HPLC is commonly practiced in high performance liquid chromatography because this technique has the advantages of versatility and appropriateness for more hydrophobic compounds. Fundamentals and Practices of Reversed-Phase HPLC: Part II -- RPHPLC column configurations. It consists of the concept of separation, types of stationary and mobile phases with the variables concerned with the separation. This revi

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Kumar, Deepak, Amrendra Kumar, Vinay Kumar, et al. "A Comprehensive Review on Analytical Method Development using RP-HPLC and Recent Advances in Pharmaceutical Applications." Journal for Research in Applied Sciences and Biotechnology 2, no. 2 (2023): 53–60. http://dx.doi.org/10.55544/jrasb.2.2.9.

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The analytical technique of choice for separating, identifying, and quantifying complex mixtures is high-performance liquid chromatography (HPLC). Reverse-phase liquid chromatography (RP-HPLC) is the preferred separation mode for high-performance liquid chromatography (HPLC) due to its adaptability and higher selectivity for hydrophobic compounds. This review article discusses the fundamentals of reversed-phase high-performance liquid chromatography (RP-HPLC). This covers the separation principle, various stationary and mobile phase types, and separation-affecting variables. This article highl

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Vinayak, A. katekar, P. Kothari Prafful, S. Kawarkhe Swapnil, P. Surung Manish, and B. Akotkar Vaishnavi. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. https://doi.org/10.5281/zenodo.8265469.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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Vinayak A. katekar, Prafful P. Kothari, Swapnil S. Kawarkhe, Manish P. Surung, and Vaishnavi B. Akotkar. "A review on method development and validation of stability indicating RP HPLC Method for metformin and Empagliflozin." GSC Biological and Pharmaceutical Sciences 24, no. 1 (2023): 310–18. http://dx.doi.org/10.30574/gscbps.2023.24.1.0233.

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Metformin HCl and empagliflozin are oral antidiabetic medications that help control blood sugar levels. A simple, precise, rapid, accurate, sensitive, specific, and stable RP-HPLC method was developed and validated for the simultaneous determination of Metformin and Empagliflozin in drug bulk form and drug dosage. This new RP-HPLC method is superior to formal reversed-phase HPLC with reduced solvent usage, retention time, resolution, and cost. Upper region separation was performed on a Waters HPLC system equipped with a PDA detector and autosampler. Both Metformin and Empagliflozin undergo str

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9

Sagar, N. Katke*1 Santosh P. Kumbhar2 Vinod D. Usnale3 Siddhant M. Sawant4. "The Method Development And Validation Of A High-Performance Liquid Chromatographic Method For Azilsartan Analysis." International Journal in Pharmaceutical Sciences 2, no. 5 (2024): 1152–61. https://doi.org/10.5281/zenodo.11240815.

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The goal of the current study is to develop an RP-HPLC-based analytical method for determining the dosage of Azilsartan in bulk and tablet form that is fast, precise, sensitive, selective, and repeatable. Create a novel HPLC technique for Azilsartan estimation, and validate it in compliance with ICH guidelines. In order to make use of the accepted methodology for Azilsartan estimation in pharmaceutical formulations, an RP-HPLC method was utilized to create and validate a stability indicating method. Using an Inertsil-ODS C18 (250×4.6mm, 5µm) column and a 90:10 v/v methanol: acetoni

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Gulabrao Bhamare, Vaibhav, and Ravindra K. Kamble. "DETERMINATION OF ETODOLAC BY RP-HPLC METHOD." International Research Journal Of Pharmacy 11, no. 9 (2020): 25–30. http://dx.doi.org/10.7897/2230-8407.110980.

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11

Anantha Kumar, D., CH Venkata Kumar, P. Seetharamaiah, and J. V. L. N. Seshagiri Rao. "Estimation of Lamotrigine by RP-HPLC Method." E-Journal of Chemistry 7, s1 (2010): S203—S208. http://dx.doi.org/10.1155/2010/754745.

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A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of lamotrigine in its pure form as well as in pharmaceutical dosage forms. Chromatography was carried out on a Luna C18column using a mixture of potassium dihydrogen phosphate buffer (pH 7.3) and methanol in a ratio of 60:40 v/v as the mobile phase at a flow rate of 1.0 mL/min. The detection was done at 305 nm. The retention time of the drug was 6.1 min. The method produced linear responses in the concentration range of 10 to 70 μg/mL of lamotrigine. The method was found

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12

Nerkar, Amit G., and Yash Patil. "A comprehensive review of analytical strategies for validating RP-HPLC methods of aceclofenac and thiocolchicoside in bulk drug and formulation." Current Trends in Pharmacy and Pharmaceutical Chemistry 6, no. 3 (2024): 84–95. http://dx.doi.org/10.18231/j.ctppc.2024.022.

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This comprehensive review explores the analytical method development and validation of Aceclofenac and Thiocolchicoside bulk drugs and formulations using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC). Aceclofenac, a nonsteroidal anti-inflammatory drug (NSAID), and Thiocolchicoside, a muscle relaxant, are commonly co-formulated for the treatment of pain and inflammation. The review discusses the principles and strategies involved in RP-HPLC method development, emphasizing factors such as stationary phase selection, mobile phase composition, and detection wavelength optimization

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Kumar, Dr Meruva Sathish, Ch Ajay Kumar, Naik Parth Pravin, N. Swarnalatha, and Afsheen Nida. "Simultaneous Estimation of Acetaminophin and Benzhydrocodone by RP-HPLC Method." International Journal of Research Publication and Reviews 5, no. 6 (2024): 6053——6062. http://dx.doi.org/10.55248/gengpi.5.0624.1620.

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14

Živančev, Dragan, Daniela Horvat, Aleksandra Torbica, et al. "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation." Journal of Chemistry 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/430328.

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RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be

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15

Harshad, Tanpure* Dr. Vishwas Bahgat Dr. Deepak Kardile Dr. Rajkumar Shete M. M. Karne. "Revolutionizing Stability-Indicating Analysis: Advanced RP-HPLC Strategies for Pharmaceutical Excellence." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 4466–89. https://doi.org/10.5281/zenodo.15522235.

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Stability-indicating analysis is vital to pharmaceutical quality control for&ensp;establishing the safety, efficacy, and quality of pharmaceutical drug products until their expiration date. Reversed-phase high-performance liquid chromatography (RP-HPLC) has recently become an important tool in stability&ensp;testing because of its high selectivity, sensitivity, and robustness. Abstract/Short: This review focused on recent developments&ensp;in RP-HPLC approaches for stability-indicating analysis to emphasize their importance in pharmaceutical excellency. Abstract The principles of stability-ind

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16

Mohammad, Tarikul Islam Bossunia, Farjana Urmi Khandokar, and Chironjit Kumar Shaha. "Quality-By-Design Approach to Stability Indicating RP-HPLC Analytical Method Development for Estimation of Canagliflozin API and Its Validation." Pharmaceutical Methods 8, no. 2 (2017): 01–10. https://doi.org/10.5281/zenodo.14856243.

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Context: Stability Indicating RP-HPLC analytical method validation for estimation of Canagliflozin API have been reported, but there are not studies related to the application of Analytical Quality by Design (AQbD) concepts to the development of a comprehensive science and risk based stability indicating RP-HPLC Analytical method for the analysis of Canagliflozin Active Pharmaceutical Ingredient (API). Aim: Development of a comprehensive science and risk based stability indicating RP-HPLC Analytical method for the analysis of Canagliflozin Active Pharmaceutical Ingredient (API) according to An

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Prajakta, G.Thete* and Ravindra B. Saudagar. "REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 05 (2018): 4897–907. https://doi.org/10.5281/zenodo.1286144.

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Analytical method development, validation, and transfer are essential elements of any pharmaceutical development program. Effective method development confirms that laboratory resources are optimized, while methods meet the objectives required at each stage of drug development. High performance liquid chromatography is most accurate methods extensively used for the qualitative and quantitative analysis of drug product. Analytical method development and validation play vital role in the drug discovery, Drug development and manufacture of pharmaceuticals. It includes detection of the purity and

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18

V, Sreehitha, R. Sireesha, B. Sivagami, V. Pavan Kumar, R. Chandrasekar, and M. Nirnajan Babu. "Simultaneous Estimation of Sofosbuvir and Velpatasvir Tablets by RP- HPLC Method." International Journal of Research and Development in Pharmacy & Life Sciences 7, no. 5 (2018): 3092–99. http://dx.doi.org/10.21276/ijrdpl.2278-0238.2018.7(5).3092-3099.

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19

Patel, Bhoomi D., Tanvi A. Nayak, and Saiyad Aaftab Hussain Tajmmul Hussain. "Development of RP-HPLC Method of Tizanidine HCL with Some Validation Parameter." Chinese Journal of Applied Physiology 40 (2024): e20240007. http://dx.doi.org/10.62958/j.cjap.2024.007.

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This study compiles the information for the development of analytical methods for estimation of the Tizanidine HCl that will be helpful for further research work on this drug and its impurity. The present Literature survey provides information about the Analytical methods like UV,TLC,RP-HPLC,HPTLC,UHPLC and other methods have been reported for Tizanidine HCl drug individually and along with other drugs. The analysis of published data revealed that, there was only UV spectroscopic method (calibration curve metod) is reported for estimation of Tizanidine HCl fixed dose combination. Estimation of

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20

G., K. Mallaiah* P. Vivek Sagar2. "Method development and Validation of Axitinib in Bulk and Pharmaceutical dosage form by RP- HPLC." Journal of Pharma Research 7, no. 12 (2018): 295–99. https://doi.org/10.5281/zenodo.6967274.

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<strong>ABSTRACT</strong> A simple, rapid, precise and accurate RP-Hplc method was developed and validated for the determination of Axitinib, in bulk and pharmaceutical dosage form. The separation is achieved on RP-HPLC using a PDA detector by incorporation of empower 2 software with a flow rate of 1.0ml/min using a mixture of Methanol and water (15:85% v/v) as mobile phase. The column used was Hypersil C18 (4.6×150mm, 5μ) at a wave length of 284nm. The retention time of the Axitinib was 3.515min. The linearity of the drug was 25-125μg/m and the method precision for the determinati

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Lohiya, G. V. Mahima Jadhav Rohini Ghotmukle Shivnechari P. M. Birajdar M. J. Kulkarni Y. P. Satpute K. L. "RP HPLC Method Development and Validation on Dapagliflozin." International Journal of Pharmaceutical Sciences 2, no. 7 (2024): 1766–79. https://doi.org/10.5281/zenodo.12806716.

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Analytical techniques encompass a broad spectrum of methods and practices essential for ensuring the quality of medicinal products. These techniques are crucial for the design, development, standardization, and quality control of pharmaceuticals. The quality of drug products is paramount because it directly impacts patient safety and treatment efficacy. Therefore, stringent manufacturing processes and quality control measures are indispensable components of robust primary healthcare systems globally.Pharmaceutical analysis, a specialized area within pharmacy, is pivotal in maintaining the qual

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Patel, Jitesha, Parin Chokshi, and Rajashree Mashru. "Validation of Stability Indicating Method and Degradation Kinetic Study of Apremilast." Journal of Drug Delivery and Therapeutics 10, no. 2 (2020): 76–85. http://dx.doi.org/10.22270/jddt.v10i2.3964.

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A novel stability indicating RP- HPLC method was developed for the estimation of Apremilast in bulk and marketed formulation. Separation was achieved by using Shimadzu HPLC Analytical Technologies Limited C18 (250 mm x 4.6 mm, 5µm) as stationary phase. The optimized mobile phase consist of potassium dihydrogen ortho phosphate (pH-3.2): acetonitrile in ratio of 40:60 %v/v with flow rate of 1mL/min by using methanol as diluent. Retention time of Apremilast was found to be 5.4 min which was estimated at wavelength 360nm. Linearity of Apremilast was observed in the concentration range of 50-400µg/

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Azka, fathima *and Shaik Gazi. "METHOD DEVELOPMENT AND VALIDATION FOR THE ANALYSIS OF CAPECITABINE BY RP-HPLC METHOD." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 08 (2018): 7808–14. https://doi.org/10.5281/zenodo.1402154.

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<em>A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Capecitabine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6×150mm, 5µm) column using a mixture of Acetonitrile: Water (50:50v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 245 nm. The retention time of the Capecitabine was 2.4 ±0.02min. The method produce linear responses in the concentration range of 15-75mg/ml of Capecitabine. The method precision for th

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Jain, Khusboo, Avneesh Sharma, and Puja Gulati. "Recent Advances in RP-HPLC Methods for Estimating Pharmaceutical Combinations: Focus on Tadalafil and Losartan Potassium." Journal of Neonatal Surgery 14, no. 12S (2025): 1035–46. https://doi.org/10.52783/jns.v14.3469.

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Recent developments in the RP-HPLC methodology aimed at determining drug combinations with special emphasis on tadalafil and losartan potassium monitoring are the subject of this research study. Tadalafil and losartan potassium receive medical usage for managing benign prostatic hyperplasia (BPH) as well as hypertension and practitioners commonly prescribe them together to treat patients with multiple health conditions. The paper demonstrates how RP-HPLC functions as the optimal analytical tool because it delivers highly precise and reproducible drug separation and quantification methods for p

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Kaushik, Patel. "Analytical method for simultaneous estimation of voglibose and mitiglinide calcium hydrate by validated RP-HPLC method." Journal of medical pharmaceutical and allied sciences 11, no. 4 (2022): 5066–71. http://dx.doi.org/10.55522/jmpas.v11i4.2646.

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A combination of Voglibose and Mitiglinide calcium hydrate is used in Type- 2 diabetes. The method was based on pre-column derivatization of Voglibose with 9-Fluorenylmethyloxycarbonyl chloride due to lack of chromophoric group it cannot directly estimated by UV detector in Reverse Phase High Performance Chromatography (RP-HPLC) method. The chromatographic separation was achieved using phosphate buffer (pH 4.0) and methanol (30:70) as mobile phase. Detection was carried out at 233 nm using UV detector. The retention time of Voglibose and Mitiglinide calcium hydrate were found to be 5.233 min a

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Varade, Priyanka R., Mahavir G. Narwade, and Varsha N. Tambe. "Method Development and Validation of Irbesartan by RP-HPLC Method." Research Journal of Science and Technology 12, no. 2 (2020): 131. http://dx.doi.org/10.5958/2349-2988.2020.00016.9.

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Mashru, Rajashree, and Manthan Trivedi. "Development and Validation of Advanced UV-Spectrophotometric Methods and a RP-HPLC Method for the Simultaneous Estimation of Beclomethasone Dipropionate and Formoterol Fumarate Dihydrate in Bulk and Pharmaceutical Dosage Forms." Journal of Drug Delivery and Therapeutics 10, no. 5 (2020): 108–17. http://dx.doi.org/10.22270/jddt.v10i5.4382.

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The presented research work aims to develop and validate UV-Spectrophotometric as well as RP-HPLC methods for the simultaneous estimation of Beclomethasone Dipropionate (BDP) and Formoterol Fumarate Dihydrate (FFD). These methods offer a higher degree of sensitivity than the already present methods of analysis. By implementing advanced spectroscopic techniques such as Dual Wavelength Method and First Derivative Spectroscopy it is found that the sensitivity of the methods is increased. The linearity of both the methods was in the range of 10µg/ml to 50µg/ml for BDP and 1µg/ml to 5µg/ml for FFD,

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V., Vishwavani *. and Dr. M. Paul Richards. "METHOD DEVELOPMENT AND VALIDATION OF CAPTOPRIL BY USING RP-HPLC." Journal of Scientific Research in Pharmacy 7, no. 4 (2018): 48–51. https://doi.org/10.5281/zenodo.1236705.

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<strong><em>ABSTRACT</em></strong> <strong><em>A</em></strong><em>n isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of captopril in API, dosage formulations and human serum. Chromatographic separation was achieved on SYMMETRY C18 150X4.6mm,3.7µm and columns using mobile phase, methanol: water (70:30 v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 1.0 mL min -1 at ambient temperature with detector set at 272 nm. Calibration curves were linear over range of 5-25 μg mL -1 with a correlation coe

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Gummadi, Sowjanya, and Rajya Lakshmi Nimmagadda. "Quantification of Pitolisant: A RP - HPLC study." YMER Digital 21, no. 06 (2022): 896–907. http://dx.doi.org/10.37896/ymer21.06/89.

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Objective An attempt was made to develop a simple, isocratic and robust reverse phase high performance liquid chromatographic method for Pitolisant. Material and methods An Inertsil octa decyl silane column and photo diode array detector were employed for the quantification of Pitolisant at 268 nm using a mobile phase consisting of acetonitrile and 0.1 % v/v formic acid (90:10 % v/v) at a flow rate of 1.0 mL/min. for the efficient elution of Pitolisant. Results The developed method was validated for various parameters and the linearity range was observed at 25-150 µg/mL. The correlation coeffi

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Keerthisikha Palur, BharathiKoganti, and Sreenivasa Charan Archakam. "Development and validation of chemometric assisted analytical methods for simultaneous estimation of Atorvastatin calcium and Aspirin in capsule dosage form." International Journal of Research in Pharmaceutical Sciences 10, no. 3 (2019): 1692–97. http://dx.doi.org/10.26452/ijrps.v10i3.1342.

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To develop two Chemometric-assisted analytical methods like UV spectrophotometry and RP-HPLC methods for the quantification of Atorvastatin calcium (ASC) and Aspirin (APN) in the capsule dosage form. Chemometric models used in UV spectrophotometry were Principal component regression model (PCRM) and Partial least-square regression (PLSR). Both the models were applied for the drugs in the calibration ranges of 4-20 and 30-150 μg/mL for ASC and APN respectively. Total of nineteen laboratory prepared mixtures were used for calibration and prediction set of the models. In addition, RP-HPLC method

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Gaikwad, Pranjal, and Vijaya Barge. "Analytical Method Development and Validation of RP-HPLC Method for Estimation of Favipiravir in Bulk and Pharmaceutical dosage form." Journal of Neonatal Surgery 14, no. 32S (2025): 4520–29. https://doi.org/10.63682/jns.v14i32s.8145.

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Objective: To develop and validate a simple, accurate, and cost-effective RP-HPLC method for the estimation of Favipiravir in bulk and pharmaceutical dosage forms. Methods: An RP-HPLC method was developed using a C18 column with methanol:water (35:65, v/v) as the mobile phase, pH 3.0, flow rate 0.8 mL/min, and UV detection at 225 nm. The method was validated as per ICH Q2(R1) guidelines for specificity, linearity, accuracy, precision, LOD, LOQ, and robustness. Forced degradation studies were conducted as per ICH Q1A(R2). Results: Favipiravir showed a retention time of 6.62 min. The method was

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Syed Ansar Ahmed and Sushil Jaysing Jadhav. "Development and Validation of Stability-Indicating RP-HPLC and UV Spectrophotometric Methods for Quantitative Determination of Hydroquinone in Pharmaceutical Formulations." Journal of Pharma Insights and Research 3, no. 3 (2025): 269–76. https://doi.org/10.69613/0dkcxn76.

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This research work focusses on two validated analytical methods for the quantification of hydroquinone in pharmaceutical formulations using reverse-phase high-performance liquid chromatography (RP-HPLC) and UV spectrophotometry. The RP-HPLC method utilized an Agilent C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of methanol and 0.05% orthophosphoric acid (50:50 v/v, pH 3.7) at a flow rate of 0.7 mL/min. Detection was performed at 290 nm with a retention time of 3.859 minutes. The UV spectrophotometric method was developed using methanol as the solvent, with measurements tak

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Wang, Haiyan, Fenglan Zhang, Jin Cao, Qingsheng Zhang, and Zhirong Chen. "Comparison of Chromatographic and Titrimetric Methods for the Determination of the a-amino Nitrogen in Standard Solution and Fish Protein Hydrolysates." Journal of Food Research 1, no. 4 (2012): 174. http://dx.doi.org/10.5539/jfr.v1n4p174.

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<p>Two analytical methods, reverse phase high-performance liquid chromatography (RP-HPLC) and formol titration, were compared for their accuracy and precision for the determination of a-amino nitrogen (a-AN) concentration in fish protein hydrolysates. The result showed that, when tested with pure amino acid solutions, RP-HPLC method had a better standard deviation (S. D.), coefficient of variation (C. V.), and standard error (S. E.) but a poorer recovery rate compared with formol titration technique. The observed a-AN value by RP-HPLC has good coincidence with the actual a-AN value and t

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Rarokar, Nilesh R., and Pramod B. Khedekar. "Stability indicating RP-HPLC method development for docetaxel trihydrate." Journal of Drug Delivery and Therapeutics 9, no. 4-s (2019): 525–28. http://dx.doi.org/10.22270/jddt.v9i4-s.3362.

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The purpose of present study was to develop RP-HPLC method for estimation of docetaxel trihydrate which should be a suitable, simple, precise, accurate, robust, and reproducible. The samples were assayed by the Shimadzu HPLC instrument - LC-20AD (Japan) equipped with Shimadzu SPD-M20A UV-VIS detector operated at wavelength of 230 nm. The binary gradiant pump was used for the analytical method development. The reverse phase stainless steel column (150 × 4.6 mm) packed with 5 μm particles (C-8, LiCrosphore® 100, Germany) was used to take chromatograph. A mobile phase consisting of acetonitrile/p

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P., Hari Sravanth Reddy *. Anusha Kota Syed Muneer. "METHOD DEVELOPMENT AND VALIDATION OF NEW RPHPLC METHOD FOR THE ESTIMATION OF PAROXETINE IN PHARMACEUTICAL DOSAGE FORM." Indo American Journal of Pharmaceutical Sciences 04, no. 07 (2017): 2151–63. https://doi.org/10.5281/zenodo.837292.

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Present study aims to develop rapid, greater sensitivity and faster elution by RP-HPLC method for the estimation of Paroxetine. The developed method will be validated in terms of accuracy, precision, linearity, robustness and ruggedness, and results will be validated statistically according to ICH guidelines. The scope of developing and validating analytical methods is to ensure suitable methods for a particular analyte of more specific, accurate, precise and robust. The main objective for this is to improve the conditions and parameters, which should be followed in the development and validat

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Bouali, Wiem, Mariem Bouali, Asena Ayse Genc, and Nevin Erk. "DEVELOPMENT AND VALIDATION OF GREEN RP-HPLC AND SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF ALPELISIB IN BULK AND PHARMACEUTICAL DOSAGE FORMS." Ankara Universitesi Eczacilik Fakultesi Dergisi 49, no. 2 (2025): 3. https://doi.org/10.33483/jfpau.1579369.

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Objective: This study aims to develop and validate green analytical methods, specifically UV spectrophotometry, first-order derivative spectrophotometry, and reverse-phase HPLC (RP-HPLC), for determining Alpelisib (ALP) in bulk and pharmaceutical formulations. By comparing the methods using GAPI and AGREE metrics, the study evaluates their environmental friendliness, precision, and applicability. Material and Method: The UV spectrophotometric and RP-HPLC analyses were conducted using Shimadzu UV 1800 and Agilent 1100 HPLC systems, respectively. The mobile phase for HPLC comprised 0.1% trifluor

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Huang, Xing, Kaipeng Zhang, Yanli Tong, Yongsheng Zhong, and Zuanguang Chen. "Determination of phospholipid and fatty glyceride in liposome by RP-HPLC with capacitively coupled contactless conductivity detector." Analytical Methods 10, no. 41 (2018): 4978–84. http://dx.doi.org/10.1039/c8ay01635f.

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In this study, a gradient RP-HPLC with capacitively coupled contactless conductivity detection (RP-HPLC-C<sup>4</sup>D) method for simultaneous determination of phospholipid and fatty glyceride in liposome was developed.

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Sharma, Bhavik, and Sushil Kumar Agarwal. "RP-HPLC Method Development and Validation for Estimation of Acebrophylline." Asian Journal of Pharmaceutical Research and Development 6, no. 6 (2019): 66–68. http://dx.doi.org/10.22270/ajprd.v6i6.445.

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Chromatography, a separation technique, is mostly used in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Theses day reversed-phase chromatography is commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. Reversed phase chromatography has found both analytical and preparative app

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Dattatray, Yadav* Dr. Deepak Kardile Dr. Vishwas Bhagat Tushar Shinde Dr. Rajkumar Shete. "Review on Advanced RP-HPLC Method Development for Multicomponent Analysis: Techniques, Applications, and Validation Protocols." International Journal of Pharmaceutical Sciences 3, no. 5 (2025): 4780–803. https://doi.org/10.5281/zenodo.15546583.

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  RP-HPLC or Reverse Phase High-Performance Liquid Chromatography is easy to use by highly trained specialists, with applications in pharmaceutical analysis, food technology, and even environmental science. It is a technology that combines two techniques, high chromatography and special compound separation. In this review, I will discuss stationary and mobile phase matters, optimization parameters for RP-HPLC systems, multi component multiseries advanced detectors, and the challenges of multi component detection using modern detectors and zeotropic chromatographic techniques. Central to n

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Shaikh, Afiya, Krunal Kanase, Vikram Veer, and Ashok Bhosale. "Analytical Method Development and Validation of RP-HPLC Method for Estimation of Levothyroxine in Bulk and Pharmaceutical Dosage Form." Journal of Neonatal Surgery 14, no. 32S (2025): 4530–39. https://doi.org/10.63682/jns.v14i32s.8146.

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Objective: To develop and validate a simple, accurate, and robust RP-HPLC method for the estimation of levothyroxine in bulk drug and pharmaceutical dosage forms, ensuring compliance with regulatory quality standards. Method: A reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed using a C18 column (250 mm × 4.6 mm, 5 µm) with a mobile phase of phosphate buffer (pH 3.0) and methanol (55:45 v/v). The flow rate was set at 1.0 mL/min, detection wavelength at 225 nm, and injection volume was 10 µL. The method was validated as per ICH Q2(R1)1guidelines. Result: The me

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Madhukar., A. *. Y. Ganesh Kumar2 K. Usha M. Srilatha. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FELODIPINE IN BULK AND TABLET DOSAGE FORM BY USING RP-HPLC TECHNIQUES." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 3248–53. https://doi.org/10.5281/zenodo.987841.

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This paper describes the analytical method suitable for validation of Felodipine by reversed Phase High Performance Liquid Chromatography (RP-HPLC) method. The method utilized RP-HPLC (Water 2695 with PDA detector) model and a column ODS C18 (4.6 x 150mm, 5m). The mobile phases were comprised with Acetonitrile and Water (80:20 V/V) at a flow rate of 1.0 ml/min. UV detection at 305 nm MTS were eluted with retention times of 3.155min. The method was continued and validated accordance with ICH guidelines. Validation revealed the method is rapid, specific, accurate, precise, reliable, and reprodu

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Y., Naveen Kumar*, Divya B., and J. Sreekanth Dr. "RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LAMIVUDINE TABLET DOSAGE FORM." Journal of Pharma Research 5, no. 15 (2016): 289–92. https://doi.org/10.5281/zenodo.14201807.

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<strong><em>ABSTRACT</em></strong> <strong><em> </em></strong>                                         <strong>A</strong>n accurate and precise HPLC method was developed for the determination of Lamivudine. Separation of the drug was achieved on a reverse phase hypersil BDS C18, 100 X 4.6 mm, 5µ using a mobile phase consisting of Buffer and acetonitril in the ratio of 65:35v/v. The flow rate

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Dandamudi, Santhoshi Priya, Ananda Kumar Chettupalli, Shanthi Priya Dargakrishna, Mounika Nerella, Ramkoteswar Rao Amara, and Vinod Kumar Yata. "Response Surface Method for the Simultaneous Estimation of Atorvastatin and Olmesartan." Trends in Sciences 19, no. 18 (2022): 5799. http://dx.doi.org/10.48048/tis.2022.5799.

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The simultaneous estimation of Atorvastatin Calcium (ATS) and Olmesartan Medoxomil (OLM) in bulk and pharmaceutical dosage forms, a new, quick, and cost-effective Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed. The experimental design was used to achieve multivariate optimization of the RP-HPLC experimental conditions. Three independent variables were used to create mathematical models: Acetonitrile content in the mobile phase composition, buffer pH, and flow rate. Here, the applied model was central composite design (CCD) to research the response surf

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Sharma, Bhavik,, and Sushil Kumar Agarwal. "RP-HPLC Method Development and Validation for Estimation of Acebrophylline." Asian Journal of Pharmaceutical Research and Development 6, no. 6 (2019): 56–59. http://dx.doi.org/10.22270/ajprd.v6i6.446.

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Acebrophylline is an anti-inflammatory and airway mucus regulator. It had ambroxol and theophylline-7-acetic acid, the former facilitates the biosynthesis of pulmonary surfactant which raises blood levels of ambroxol, by stimulating surfactant production. Chemical structure of acebrophylline is 1, 2, 3, 6- tetrahydro-1, 3-dimethyl-2, 6-dioxo-7H-purine-7-aceticacidwithtrans-4-[(2-amino-3, 5 dibromophenyl) methyl] aminio] cyclohexanol. Survey revealed that various analytical methods like spectrophotometric, HPLC, and RP-HPLC, have been reported for the determination of Ambroxol HCl and Theophyll

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Y., Naveen Kumar, Divya B., and J. Sreekanth Dr. "ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF ZIDOVUDINE TABLET DOSAGE FORM BY RP-HPLC." Journal of Pharma Research 7, no. 12 (2018): 338–41. https://doi.org/10.5281/zenodo.14227193.

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<strong>Abstract</strong> <strong>A</strong>n accurate and precise HPLC method was developed for the Simultaneous determination of Zidovudine. Separation of the drug was achieved on a reverse phase hypersil BDS C18, 100 X 4.6 mm, 5µ using a mobile phase consisting of Buffer and acetonitril in the ratio of 65:35v/v. The flow rate was 1mL/min and the detection wavelength was 270nm. The linearity was observed in the range of 25% to 150% with a correlation coefficient of not less than 0.998. The %RSD of Zidovudinein precision was found to be 0.20, 0.05 respectively. The recovery was found to

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Ankush Bhalerao, Mr. Satish Shelke, and Dr. Vijay Borkar. "Advances, Applications, and Challenges in RP HPLC Method Development." International Journal of Advanced Research in Science, Communication and Technology, April 3, 2023, 111–23. http://dx.doi.org/10.48175/ijarsct-9017.

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High-performance liquid chromatography (HPLC) has become an indispensable tool for modern analytical chemistry, and reversed-phase (RP) HPLC is one of the most widely used techniques for the separation and analysis of complex mixtures. In recent years, there have been numerous advances in RP HPLC method development, including the development of new stationary phases, improved column technology, and novel optimization strategies. These advances have led to increased speed, resolution, and sensitivity, as well as improved selectivity and reduced non-specific adsorption. Moreover, RP HPLC has fou

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Miss., J. V. More. "Review on Reversed-phase High-performance Liquid Chromatography Method Development and Validation for Estimation of Rivaroxaban." February 4, 2021. https://doi.org/10.5281/zenodo.4500602.

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This review article is proposed to underline method for the development by reversed-phase highperformance liquid chromatography (RP-HPLC) and validation of rivaroxaban in individual and pharmaceutical dosage form. Rivaroxaban is an anti-clotting drug, acts on Factor Xa, and stops the blood clot development. In this study, different types of RP-HPLC methods which are available at present for the determination of rivaroxaban in tablets (Xarelto 10 mg) are studied. There are different types of the methods described for the estimation of this drug such as RP-HPLC, ultra performance liquid chromato

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Prajapati, Pintu, Ankita Patel, and Shailesh Shah. "Application of DoE-Based Analytical QRM to Development of the Multipurpose RP-HPLC Method for Estimation of Multiple FDC Products of Telmisartan Using Enhanced AQbD Approach." Journal of Chromatographic Science, June 14, 2021. http://dx.doi.org/10.1093/chromsci/bmab070.

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Abstract Telmisartan is an antihypertensive drug and several FDC products of telmisartan are available in the market for the treatment of hypertension. The multipurpose reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed and validated as economical and eco-friendly alternative to numerous published chromatography methods for synchronous estimation of FDC of telmisartan to save time, cost and solvent for analysis. The analytical quality risk management was initiated with identification of potential method parameters using cause–effect diagram followed by ass

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Nagulancha, Bhujanga Rao, and Vandavasi Koteswara Rao. "Stability‐indicating method for quantification of potential genotoxic impurity and other organic impurities of chlorzoxazone using hyphenated techniques." SEPARATION SCIENCE PLUS, November 2, 2023. http://dx.doi.org/10.1002/sscp.202300138.

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AbstractA simple, reliable, and stability‐indicating RP‐HPLC‐UV method was developed and validated for the estimation of related substances (p‐chlorophenol and unknown impurities) of chlorzoxazone (skeletal muscle relaxant). Along with this as the compound contains a potential genotoxic impurity (2‐amino‐4‐chlorophenol), which needs to have more sensitivity in quantification, we have also developed and validated a separate RP‐HPLC‐mass spectrometry (MS)/MS method for the estimation of 2‐amino‐4‐chlorophenol impurity. Both methods (RP‐HPLC‐UV & RP‐HPLC–MS/MS) were validated in accordance wi

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Vaidehi, Sunil Holey, and G. Jawarkar Shailesh. "Analytical Method Development and Validation of Stability Indicating RP-HPLC Method For Imeglimin Hydrochloride." April 7, 2024. https://doi.org/10.5281/zenodo.11140590.

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ABSTRACT The present study describes the development and subsequent validation of Reverse phase HPLC (RP-HPLC) method for the analysis of Imeglimin hydrochloride. A novel economic, simple, rapid, accurate, reproducible, and precise Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method for Imeglimin hydrochloride. The method was performed on a YOUNG LIN-HPLC system-ACME9000. The method developed for Imeglimin hydrochloride was quantitatively measured using an isocratic RP-HPLC methodology. The chromatographic separation of Imeglimin hydrochloride was achieved on RP-HPLC equipped

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