Relevant bibliographies by topics / RP-HPLC: reversed-phase HPLC

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Academic literature on the topic 'RP-HPLC: reversed-phase HPLC'

Author: Grafiati
Published: 4 June 2021
Last updated: 5 February 2022

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Journal articles on the topic "RP-HPLC: reversed-phase HPLC"

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Sharma, Bhavik, and Sushil Kumar Agarwal. "RP-HPLC Method Development and Validation for Estimation of Acebrophylline." Asian Journal of Pharmaceutical Research and Development 6, no. 6 (2019): 66–68. http://dx.doi.org/10.22270/ajprd.v6i6.445.

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Chromatography, a separation technique, is mostly used in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Theses day reversed-phase chromatography is commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. Reversed phase chromatography has found both analytical and preparative app
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KRUGER, J. E., and B. A. MARCHYLO. "SELECTION OF COLUMN AND OPERATING CONDITIONS FOR REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY OF PROTEINS IN CANADIAN WHEAT." Canadian Journal of Plant Science 65, no. 2 (1985): 285–98. http://dx.doi.org/10.4141/cjps85-041.

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Chromatographic conditions were optimized and three commercially available columns were evaluated for separation of alcohol-soluble storage proteins of Neepawa wheat using reversed-phase high-performance liquid chromatography (RP-HPLC). Optimal separation was achieved using an extracting solution of 50% 1-propanol, 1% acetic acid, and 4% dithiothreitol and an HPLC elution time of 105 min at a flow rate of 1.0 mL/min. HPLC columns evaluated (SynChropak RP-P, Ultrapore RPSC and Aquapore RP-300) varied in selectivity and resolution. The column providing the greatest versatility was Aquapore RP-30
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Harenberg, J., and R. Malsch. "Analyse von Heparinoiden mit Galaktosaminoglykanstruktur mit Hilfe der Hochdruckflüssigkeitschromatographie." Hämostaseologie 16, no. 01 (1996): 15–20. http://dx.doi.org/10.1055/s-0038-1656633.

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ZusammenfassungHeparinoide mit Galaktosaminoglykanstruktur können mit chromatographischen Methoden auf ihre Eigenschaften untersucht werden. Mit Hilfe der Hochdruck-flüssigkeits-Gelpermeationschromatographie (GPC-HPLC) wurden daher die durchschnittliche molekulare Masse und die Polydispersität (Q) bestimmt. Die Stoffe zeigten eine unterschiedliche durchschnittliche molekulare Masse Mw von 19077 bis 25193 Da. Die Polydispersität streute von 1,04 bis 1,13 für unfraktionierte Galaktosaminoglykane. Die Reversed-Phase-Hochdruckflüssig-keitschromatographie (RP-HPLC) läßt Unterschiede in der Lipophil
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Pinho, Carina, Zita E. Martins, Catarina Petisca, Agata M. Figurska, Olívia Pinho, and Isabel M. P. L. V. O. Ferreira. "Size exclusion and reversed-phase high-performance liquid chromatography/UV for routine control of thermal processing of cows' and donkey milk major proteins." Journal of Dairy Research 79, no. 2 (2012): 224–31. http://dx.doi.org/10.1017/s0022029912000064.

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Cows' and donkey milks (raw and thermally processed) and respective whey were analysed for quantification of major proteins. Two different chromatographic approaches, size exclusion (SE-HPLC) and reversed-phase high performance liquid chromatography (RP-HPLC) both coupled to UV detection were used. Usefulness of these methods for routine control of the effect of thermal processing was evaluated. The external standard method was used to calibrate the SE-HPLC and RP-HPLC systems. Concerning quantification of β-lactoglobulin (β-lg), α-lactalbumin (α-la), lysozyme (lys), and total casein (cn), no
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Živančev, Dragan, Daniela Horvat, Aleksandra Torbica, et al. "Benefits and Limitations of Lab-on-a-Chip Method over Reversed-Phase High-Performance Liquid Chromatography Method in Gluten Proteins Evaluation." Journal of Chemistry 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/430328.

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RP-HPLC (reversed-phase high-performance liquid chromatography) is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoaC (Lab-on-a-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. In the present study, benefits and limitations of Lab-on-a-Chip method over RP-HPLC method in gluten proteins evaluation were explored in order to determine in which way LoaC method should be
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Biancolillo, Alessandra, Maria Anna Maggi, Sebastian Bassi, Federico Marini, and Angelo Antonio D’Archivio. "Retention Modelling of Phenoxy Acid Herbicides in Reversed-Phase HPLC under Gradient Elution." Molecules 25, no. 6 (2020): 1262. http://dx.doi.org/10.3390/molecules25061262.

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Phenoxy acid herbicides are used worldwide and are potential contaminants of drinking water. Reversed phase high-performance liquid chromatography (RP-HPLC) is commonly used to monitor phenoxy acid herbicides in water samples. RP-HPLC retention of phenoxy acids is affected by both mobile phase composition and pH, but the synergic effect of these two factors, which is also dependent on the structure and pKa of solutes, cannot be easily predicted. In this paper, to support the setup of RP-HPLC analysis of phenoxy acids under application of linear mobile phase gradients we modelled the simultaneo
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Attia, Khalid A. M., Mohammed W. I. Nassar, Mohamed B. El-Zeiny, and Ahmed Serag. "Molecular and Quantum Mechanical Study for the Separation of Cefprozil in the Presence of Its Alkaline Degradation Product Using RP-HPLC with UV Detection." Journal of AOAC INTERNATIONAL 100, no. 2 (2017): 392–99. http://dx.doi.org/10.5740/jaoacint.16-0246.

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Abstract A reversed-phase HPLC method (RP-HPLC) with UV detection was developed and validated for the quantitative determination of cefprozil, a second-generation cephalosporin. Due to β-lactam ring instability under alkaline conditions, this RP-HPLC method was applied for the determination of cefprozil in the presence of its possible degradation product. The interactions that govern the separation process with stationary phase were investigated at both molecular and quantum mechanical levels. Moreover, electrostatic potential maps were generated to determine the sites of interaction with mobi
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Cholaraja, Bijithra, Shanmugasundaram P, Ragan G, Sankar Ask, and Sumithra M. "ANALYITCAL METHOD DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HIGHPERFORMANCE LIQUID CHROMATOGRAPHY FOR THE DETERMINATION OF MODAFINIL IN BULK AND PHARMACEUTICAL DOSAGE FORMS." Asian Journal of Pharmaceutical and Clinical Research 9, no. 5 (2016): 177. http://dx.doi.org/10.22159/ajpcr.2016.v9i5.13178.

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ABSTRACTObjective: To development and validation of a reversed-phase high-performance liquid chromatography (RP-HPLC) for the determination of modafinilin bulk and pharmaceutical dosage forms.Methods: A simple, precise, rapid, and accurate RP-HPLC method was developed for the estimation of modafinil in bulk and pharmaceutical dosageforms. Xterra RP 18 (250 mm × 4.6 mm, 5 µ particle size) with a mobile phase consisting of methanol:water 70:30 V/V was used. The flow rate1.0 ml/min and the effluents were monitored at 260 nm. The retention time and recovery time was 12 minutes. The detector respon
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B, Siva Sai Kiran, and Raja S. "Development of validated method for the determination of exemestane by using RP-HPLC." International Journal of Research in Pharmaceutical Sciences 9, no. 1 (2018): 185. http://dx.doi.org/10.26452/ijrps.v9i1.1235.

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A novel RP-HPLC validated method for determination of Exemestane is developed. The chromatographic separation was done on Phenomenex Luna reversed phase C18 (150 mm x 4.6 mm, 5 μm) column in isocratic mode, using Acetonitrile: HPLC grade water (50:50, v/v) as mobile phase at 254 nm wavelength. Exemestane chromatographic peak is eluted with retention time 4.656 min. The linearity range was 5-30 μg/ml with correlation coefficient 0.999. The method was validated as per ICH Guidelines. The quantification and detection limit for estimation of Exemestane was found to be 0.365 and 1.926 μg/ml respect
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Venta, Rafael. "Year-Long Validation Study and Reference Values for Urinary Amino Acids Using a Reversed-Phase HPLC Method." Clinical Chemistry 47, no. 3 (2001): 575–83. http://dx.doi.org/10.1093/clinchem/47.3.575.

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Abstract Background: Reversed-phase HPLC (RP-HPLC) has become an alternative to ion-exchange chromatography for amino acid analysis in biological fluids. However, validation studies for its urine application are limited, and the corresponding reference values have not been reported extensively. We studied the long-term performance of a commercial HPLC method for urine amino acid analysis and established specific age-related reference values for urine amino acid excretion. Methods: Method performance was continuously assessed by recovery and precision studies with urine samples and controls, re
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Dissertations / Theses on the topic "RP-HPLC: reversed-phase HPLC"

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Watson, Richard Charles. "Studies of reversed phase high performance liquid chromatography (RP-HPLC) stationary phases." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338492.

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Alshana, Usama Ahmed. "Separation And Quantitation Of Some Platinum Group Metals By Rp-hplc." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12605760/index.pdf.

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In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed to separate and determine Pt and Pd after formation of their chelates with N,N-diethyl-N&#039<br>-benzoylthiourea (DEBT). With the aim of reducing the number of steps in treating the samples, the method developed does not require the elimination of excess chelating reagent before the analysis of metal chelates. The different physical and chemical parameters affecting separation were examined in details. The whole analysis was completed on a C18 column in 16 min at 280 nm, with the mobile
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Mautjana, Nare Alpheus. "Determination of platinum, palladium and rhodium, in aqueous media by means of reversed phase-high performance liquid chromatography (RP-HPLC) after complexation with N,N-dialkyl-N'-acylthioureas." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/11809.

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Includes bibliographical references.<br>The complexes were successfully separated using conventional Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) and a UV detector. A simple method of complexation of platinum(II) and palladium(II) in acidic, aqueous samples was developed. This method involves the mixing of excess ligand (HL") in acetonitrile with aqueous solutions containing traces of platinum(II) and palladium(II) at room temperature followed by salt-induced phase separation prior to RP-HPLC analysis. In the case of rhodium(III), however, complexation was extremely slow and
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(5929574), Yuan Chen. "PHOTOLYTIC LABELING TO PROBE PEPTIDE-MATRIX INTERACTIONS IN LYOPHILIZED SOLIDS." Thesis, 2020.

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<p>Therapeutic proteins are often lyophilized with excipients such as sucrose or trehalose to protect them during manufacturing and achieve a longer shelf-life. Formulation design for therapeutic proteins has been a trial-and-error process, and the mechanisms responsible for the stabilizing effects of excipients are not fully understood. Two proposed theories have been widely accepted: the water replacement theory and the vitrification theory.<sup>1,2</sup>The water replacement theory suggests that excipients stabilize protein molecules in the solid state by forming hydrogen bonds that “replac
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Book chapters on the topic "RP-HPLC: reversed-phase HPLC"

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van de Waterbeemd, H., M. Kansy, B. Wagner, and H. Fischer. "Lipophilicity Measurement by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC)." In Lipophilicity in Drug Action and Toxicology. Wiley-VCH Verlag GmbH, 2008. http://dx.doi.org/10.1002/9783527614998.ch5.

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"Reversed Phase HPLC: Determination of Variety Musts by SPE/RP-HPLC Analysis of Their Soluble Proteins." In Handbook of Advanced Materials Testing. CRC Press, 1994. http://dx.doi.org/10.1201/9781482277616-19.

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