Academic literature on the topic 'RPE in-Cell'

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Journal articles on the topic "RPE in-Cell"

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Kuznetsova, Alla V., Alexander M. Kurinov, and Maria A. Aleksandrova. "Cell Models to Study Regulation of Cell Transformation in Pathologies of Retinal Pigment Epithelium." Journal of Ophthalmology 2014 (2014): 1–18. http://dx.doi.org/10.1155/2014/801787.

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The retinal pigment epithelium (RPE) plays a key role in the development of many eye diseases leading to visual impairment and even blindness. Cell culture models of pathological changes in the RPE make it possible to study factors responsible for these changes and signaling pathways coordinating cellular and molecular mechanisms of cell interactions under pathological conditions. Moreover, they give an opportunity to reveal target cells and develop effective specific treatment for degenerative and dystrophic diseases of the retina. In this review, data are presented on RPE cell sources for cu
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Marrs, J. A., C. Andersson-Fisone, M. C. Jeong, et al. "Plasticity in epithelial cell phenotype: modulation by expression of different cadherin cell adhesion molecules." Journal of Cell Biology 129, no. 2 (1995): 507–19. http://dx.doi.org/10.1083/jcb.129.2.507.

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A primary function of cadherins is to regulate cell adhesion. Here, we demonstrate a broader function of cadherins in the differentiation of specialized epithelial cell phenotypes. In situ, the rat retinal pigment epithelium (RPE) forms cell-cell contacts within its monolayer, and at the apical membrane with the neural retina; Na+, K(+)-ATPase and the membrane cytoskeleton are restricted to the apical membrane. In vitro, RPE cells (RPE-J cell line) express an endogenous cadherin, form adherens junctions and a tight monolayer, but Na+,K(+)-ATPase is localized to both apical and basal-lateral me
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Zhou, Liangliang, Ya Mo, Haiyan Zhang, Mengdi Zhang, Jiayu Xu, and Sumin Liang. "Role of AMPK-regulated autophagy in retinal pigment epithelial cell homeostasis: A review." Medicine 103, no. 28 (2024): e38908. http://dx.doi.org/10.1097/md.0000000000038908.

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The retinal pigment epithelium (RPE) is a regularly arranged monolayer of cells in the outermost layer of the retina. It is crucial for transporting nutrients and metabolic substances in the retina and maintaining the retinal barrier. RPE dysfunction causes diseases related to vision loss. Thus, understanding the mechanisms involved in normal RPE function is vital. Adenosine monophosphate-activated protein kinase (AMPK) is an RPE energy sensor regulating various signaling and metabolic pathways to maintain cellular energetic homeostasis. AMPK activation is involved in multiple signaling pathwa
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Voisin, Audrey, Christelle Monville, Alexandra Plancheron, Emile Béré, Afsaneh Gaillard, and Nicolas Leveziel. "Cathepsin B pH-Dependent Activity Is Involved in Lysosomal Dysregulation in Atrophic Age-Related Macular Degeneration." Oxidative Medicine and Cellular Longevity 2019 (December 6, 2019): 1–15. http://dx.doi.org/10.1155/2019/5637075.

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Age-related macular degeneration (AMD) is characterized by retinal pigment epithelial (RPE) cell dysfunction beginning at early stages of the disease. The lack of an appropriate in vitro model is a major limitation in understanding the mechanisms leading to the occurrence of AMD. This study compared human-induced pluripotent stem cell- (hiPSC-) RPE cells derived from atrophic AMD patients (77 y/o±7) to hiPSC-RPE cells derived from healthy elderly individuals with no drusen or pigmentary alteration (62.5 y/o±17.5). Control and AMD hiPSC-RPE cell lines were characterized by immunofluorescence, f
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Kindzelskii, Andrei L., Victor M. Elner, Susan G. Elner, Dongli Yang, Bret A. Hughes, and Howard R. Petty. "Toll-Like Receptor 4 (TLR4) of Retinal Pigment Epithelial Cells Participates in Transmembrane Signaling in Response to Photoreceptor Outer Segments." Journal of General Physiology 124, no. 2 (2004): 139–49. http://dx.doi.org/10.1085/jgp.200409062.

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Retinal pigment epithelial (RPE) cells mediate the recognition and clearance of effete photoreceptor outer segments (POS), a process central to the maintenance of normal vision. Given the emerging importance of Toll-like receptors (TLRs) in transmembrane signaling in response to invading pathogens as well as endogenous substances, we hypothesized that TLRs are associated with RPE cell management of POS. TLR4 clusters on human RPE cells in response to human, but not bovine, POS. However, TLR4 clustering could be inhibited by saturating concentrations of an inhibitory anti-TLR4 mAb. Furthermore,
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Szatmári-Tóth, Mária, Tanja Ilmarinen, Alexandra Mikhailova, et al. "Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium-Role in Dead Cell Clearance and Inflammation." International Journal of Molecular Sciences 20, no. 4 (2019): 926. http://dx.doi.org/10.3390/ijms20040926.

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Inefficient removal of dying retinal pigment epithelial (RPE) cells by professional phagocytes can result in debris formation and development of age-related macular degeneration (AMD). Chronic oxidative stress and inflammation play an important role in AMD pathogenesis. Only a few well-established in vitro phagocytosis assay models exist. We propose human embryonic stem cell-derived-RPE cells as a new model for studying RPE cell removal by professional phagocytes. The characteristics of human embryonic stem cells-derived RPE (hESC-RPE) are similar to native RPEs based on their gene and protein
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Daniele, Elena, Lorenzo Bosio, Noor Ahmed Hussain, et al. "Denuded Descemet’s membrane supports human embryonic stem cell-derived retinal pigment epithelial cell culture." PLOS ONE 18, no. 2 (2023): e0281404. http://dx.doi.org/10.1371/journal.pone.0281404.

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Recent clinical studies suggest that retinal pigment epithelial (RPE) cell replacement therapy may preserve vision in retinal degenerative diseases. Scaffold-based methods are being tested in ongoing clinical trials for delivering pluripotent-derived RPE cells to the back of the eye. The aim of this study was to investigate human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells survival and behaviour on a decellularized Descemet’s Membrane (DM), which may be of clinical relevance in retinal transplantation. DMs were isolated from human donor corneas and treated with ther
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Hellinen, Laura, Heidi Hongisto, Eva Ramsay, et al. "Drug Flux Across RPE Cell Models: The Hunt for An Appropriate Outer Blood–Retinal Barrier Model for Use in Early Drug Discovery." Pharmaceutics 12, no. 2 (2020): 176. http://dx.doi.org/10.3390/pharmaceutics12020176.

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The retinal pigment epithelial (RPE) cell monolayer forms the outer blood–retinal barrier and has a crucial role in ocular pharmacokinetics. Although several RPE cell models are available, there have been no systematic comparisons of their barrier properties with respect to drug permeability. We compared the barrier properties of several RPE secondary cell lines (ARPE19, ARPE19mel, and LEPI) and both primary (hfRPE) and stem-cell derived RPE (hESC-RPE) cells by investigating the permeability of nine drugs (aztreonam, ciprofloxacin, dexamethasone, fluconazole, ganciclovir, ketorolac, methotrexa
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Kamao, Hiroyuki, Atsushi Miki, and Junichi Kiryu. "ROCK Inhibitor-Induced Promotion of Retinal Pigment Epithelial Cell Motility during Wound Healing." Journal of Ophthalmology 2019 (June 19, 2019): 1–10. http://dx.doi.org/10.1155/2019/9428738.

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Purpose. No standard therapy for RPE tear, a complication of neovascular age-related macular degeneration, exists even though RPE tears cause severe vision loss, and promotion of cell proliferation and/or migration could be a candidate RPE tear therapy. The aim of this study is to evaluate the effect of Rho-associated coiled-coil containing kinase (ROCK) inhibitor Y27632 on retinal pigment epithelial (RPE) cell motility during wound healing. Methods. Human RPE cells were cultured in media with and without 10 μM Y27632. A luminescent cell viability assay and vinculin immunocytochemistry were us
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Bhattacharya, Sujoy, Tzushan Sharon Yang, Bretton P. Nabit, Evan S. Krystofiak, Tonia S. Rex, and Edward Chaum. "Prominin-1 Knockdown Causes RPE Degeneration in a Mouse Model." Cells 13, no. 21 (2024): 1761. http://dx.doi.org/10.3390/cells13211761.

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There are currently no effective treatments for retinal pigment epithelial (RPE) cell loss in atrophic AMD (aAMD). However, our research on Prominin-1 (Prom1), a known structural protein in photoreceptors (PRs), has revealed its distinct role in RPE and offers promising insights. While pathogenic Prom1 mutations have been linked to macular diseases with RPE atrophy, the broader physiological impact of dysfunctional Prom1 in RPE loss is unclear. We have shown that Prom1 plays a crucial role in regulating autophagy and cellular homeostasis in human and mouse RPE (mRPE) cells in vitro. Neverthele
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Dissertations / Theses on the topic "RPE in-Cell"

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Spencer, Samantha A. "The Role of tfec in Zebrafish Neural Crest Cell and RPE Development." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3754.

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Zebrafish (Danio rerio) show a unique pigmentation pattern comprised of three pigment cell types: melanophores, iridophores and xanthophores. Other pigmented cells include the retinal pigmented epithelium (rpe) which absorbs excess light in the eye and maintain the extracellular environment around the photoreceptors. While previous mutations in mitfa showed a role in regulating trunk melanophores, the rpe was not affected. TALENs and CRISPR-Cas9 systems were used to generate mutant zebrafish for tfec, a transcription factor expressed in both neural crest and rpe. Embryos with tfec mutations
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Gentles, Jeremy A., William G. Hornsby, Howard S. Gray, et al. "Changes in Cell Free DNA During a College Soccer Season." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/3795.

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Objectives: This study investigated chronic changes in cell free DNA (cf-DNA) throughout a collegiate soccer season. The relationship between cf-DNA, C-reactive protein (CRP), creatine kinase (CK), testosterone (T), cortisol (C), testosterone-cortisol ratio (T:C), body mass and body composition were also examined. Design: Longitudinal study design with repeated measures and group comparisons.Methods: Twenty three NCAA Division I male soccer players were divided into two groups. Starters were placed in Group 1 (G1) and non-starters were placed in Group 2 (G2). cf-DNA, CRP, CK, T, C, T:C, body m
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Pierro, Annalisa. "Protein structural dynamics in bacteria via nitroxide-based SDSL-EPR spectroscopy : from method improvements to in-cell studies." Electronic Thesis or Diss., Aix-Marseille, 2021. http://theses.univ-amu.fr.lama.univ-amu.fr/211116_PIERRO_290xrxu60ryzjfl293g970fjmdnl_TH%20(1).pdf.

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L'étude des biomolécules dans leur environnement natif, la cellule, est l'un des principaux objectifs de la biologie structurale au cours de la dernière décennie. Ainsi, nous assistons à un remarquable développement des approches dites « in-cell », comme la CryoET, FRET (Förster Resonance Energy Tranfer), RMN. Parmi elles, la technique de marquage de spin couplée à la spectroscopie de Résonance Paramagnétique Electronique (SDSL-RPE) présente des caractéristiques intéressantes et avantageuses permettant de sonder la dynamique des protéines à l'intérieur des cellules. En particulier, l’utilisati
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Guo, Shuangli. "ROLE OF REPLICATION PROTEIN A (RPA) AND PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN DNA MISMATCH REPAIR." UKnowledge, 2005. http://uknowledge.uky.edu/gradschool_diss/258.

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PCNA and RPA are required for DNA mismatch repair (MMR), but their rolesin the pathway are not fully understood. Using an affinity pull-down approach, weshow that (1) increased PCNA binding to DNA heteroduplexes is associated withthe appearance and accumulation of excision products; and (2) RPAphosphorylation occurs when DNA polymerase ?? binds to the DNA substrate. Wetherefore hypothesize that PCNA plays an important role in mismatch-provokedexcision and that RPA phosphorylation plays an important role in DNA resynthesis.To determine the role of PCNA in MMR, mismatch-provoked and nick-directe
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Guo, Shuangli. "Role of DNA replication proteina (RPA) and proliferating cell nuclear antigen (PCNA) in human DNA mismatch repair." Lexington, Ky. : [University of Kentucky Libraries], 2005. http://lib.uky.edu/ETD/ukybioc2005d00284/etd.pdf.

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Thesis (Ph. D.)--University of Kentucky, 2005.<br>Title from document title page (viewed on November 7, 2005). Document formatted into pages; contains x, 3 p. : ill. Includes abstract and vita. Includes bibliographical references (p. 84-92).
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Davoren, Jonathan M., and University of Lethbridge Faculty of Arts and Science. "Gene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil production." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 1997, 1997. http://hdl.handle.net/10133/345.

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Triacylglycerol (TAG) production in the microspore derived (MD) cell suspension culture ofBrassica napus L. cv Jet Neuf was enhanced when the sucrose concentration in the growth medium was increased from 2 to 14 % (w/v). mRNA differential display by polymerase chain reaction was used to examine gene expression in cells grown at different sucrose concentrations in order to identify mRNAs which could be associated with oil formation. The anchored primer, T12AA, was used to screen one subset, representing approximately one twelfth of the transcript population, isolated from cultures grown in medi
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Pourazadi, Ehsan. "Facile Synthesis of Boron-doped Graphitic Materials for Oxygen Reduction Purpose in Fuel Cell." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16032.

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Modern civilization is blamed for aggravating climate change as well as global warming by pumping millions of tonnes of greenhouse gases into the atmosphere. In addition to environmental concerns, the ever-increasing energy demand calls for new generations of power technologies to supply such a rapid rise in energy market. Fuel cells show to be placed at the cornerstone of technology development for the current century, since they could mitigate the environmental concern and meet the escalation in energy need. However, transforming to a future sustainable energy with zero to low CO2 emission n
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Wolk, Alyson M. "The Role of the Retinal Pigment Epithelium in Sorsby Fundus Dystrophy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1606842751125309.

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Du, Plessis Peter Clark. "Variations in radiosensitivity of breast cancer and normal breast cell lines using a 200MeV clinical proton beam." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2970.

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Thesis (MSc (Radiography))--Cape Peninsula University of Technology, 2018<br>Background: Breast cancer is one of the most commonly diagnosed among woman in South Africa, and a more resilient effort should be focused on treatment improvements. Worldwide, proton therapy is increasingly used as a radiation treatment alternative to photon therapy for breast cancer, mostly to decrease the risk for radiation-induced cardiovascular toxicity. This in vitro study aims to determine a better understanding of the radiosensitivity of both tumour and normal breast cell lines to clinical proton irradiation.
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Shani, Saeideh. "The implication of cell-derived microvesicles in retinal pigment epithelium degeneration." Thèse, 2018. http://hdl.handle.net/1866/22150.

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Books on the topic "RPE in-Cell"

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Bengtsson, Staffan. Studies on structures and properties of soluble cell-well polysaccharides in rye and barley. Swedish University of Agricultural Sciences, Dept. of Food Science, 1991.

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Rape in Holding Cell 6. Nazca Plains Corporation, The, 2011.

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Rape In Holding Cell 6. Nazca Plains Corporation, 2010.

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Sullivan, Kyle Michel. Rape in Holding Cell 6. KMSCB, 2014.

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Monoclonal antibodies: Principles and practice : production and application of monoclonal antibodies in cell biology, biochemistry, and immunology. 2nd ed. Academic Press, 1986.

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McGough, Michael. A Field Guide to the Culture Wars. Greenwood Publishing Group, Inc, 2008. http://dx.doi.org/10.5040/9798400650796.

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Like any realignment in politics, the Democratic takeover of Congress in the 2006 midterm elections inspired a raft of instant analyses. One take on the results that is surely wrong is that the change in control of Congress and the spike in Democratic hopes for the 2008 presidential race mark an end to the culture wars that conventional wisdom blamed (or credited) for George W. Bush's re-election in 2004. This book sets the stage for a new consideration of the contemporary culture wars by examining their antecedents—from the Scopes trial to Prohibition to the controversy over the Supreme Court
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Glausser, Wayne. Entanglement. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190864170.003.0001.

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This introductory chapter offers a brief definition of entanglement and contrasts it with four other versions of the relationship between religion and secularity. Unlike these other four models, in which religion and secularity sit apart from each other as separate domains, entanglement presents a contentious but oddly intimate relationship. Neither side simply wins by displacing the other. Two examples flesh out this definition of entangled religious and secular interests. The first example comes from the so-called War on Christmas; the second comes from controversies surrounding stem cell re
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Izzedine, Hassan, and Victor Gueutin. Drug-induced acute tubulointerstitial nephritis. Edited by Adrian Covic. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0084.

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Drug-induced acute tubulointerstitial nephritis (ATIN) is the most common aetiology of ATIN and a potentially correctable cause of acute kidney injury (AKI). An interval of 7–10 days typically exists between drug exposure and development of AKI, but this interval can be considerably shorter following re-challenge or markedly longer with certain drugs. It occurs in an idiosyncratic and non-dose-dependent manner. Antibiotics, NSAIDs, and proton pump inhibitors are the most frequently involved agents, but the list of drugs that can induce ATIN is continuously increasing. The mechanism of renal in
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Tallacchini, Mariachiara. Medical Technologies and EU Law: The Evolution of Regulatory Approaches and Governance. Oxford University Press, 2017. http://dx.doi.org/10.1093/acprof:oso/9780198807216.003.0002.

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The regulatory evolution of medical technologies in the EU offers a unique perspective with regard to highlighting significant elements of both European science policy and the development of European institutions, especially with regard to the passage from their (primarily) economic to their political phases. Since the early 1990s, while establishing a market for biotechnology, the European Communities have been developing some policy-related visions of technoscience and its potential risks, while at the same time framing the concept of European citizenship through European values and rights.
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Snyder, Jeremy. Exploiting Hope. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780197501252.001.0001.

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One often hears stories of people in terrible and seemingly intractable situations who are preyed upon by individuals offering empty promises of help. Frequently these cases are condemned as “exploiting the hope” of another. These accusations are made in a range of contexts, including human smuggling, the beauty industry, and unproven medical interventions. This concept is meant to do heavy lifting in public discourse, identifying a specific form of unethical conduct. However, it is poorly understood what is meant to be wrong by the accusation of exploiting hope, the range of activities that c
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Book chapters on the topic "RPE in-Cell"

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Qin, Suofu. "Roles for AMP-Activated Protein Kinase in RPE Cell Function." In Retinal Degenerative Diseases. Springer US, 2011. http://dx.doi.org/10.1007/978-1-4614-0631-0_95.

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Rashid, Alia, Shagun K. Bhatia, Karina I. Mazzitello, et al. "RPE Cell and Sheet Properties in Normal and Diseased Eyes." In Retinal Degenerative Diseases. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-17121-0_101.

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Khristov, Vladimir, Arvydas Maminishkis, Juan Amaral, Aaron Rising, Kapil Bharti, and Sheldon Miller. "Validation of iPS Cell-Derived RPE Tissue in Animal Models." In Retinal Degenerative Diseases. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-75402-4_77.

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Yamada, H., N. Ogata, C. Yamamoto, M. Miyashiro, M. Uyama, and A. Del Monte. "Localization of bFGF in wound healing process of RPE cell in vitro." In Documenta Ophthalmologica Proceedings Series. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5137-5_13.

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Hazim, Roni, Mei Jiang, Julian Esteve-Rudd, Tanja Diemer, Vanda S. Lopes, and David S. Williams. "Live-Cell Imaging of Phagosome Motility in Primary Mouse RPE Cells." In Retinal Degenerative Diseases. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-17121-0_100.

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Skottman, Heli. "RPE and Stem Cell Therapy." In Retinal Pigment Epithelium in Health and Disease. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-28384-1_14.

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Detrick, Barbara, and John J. Hooks. "The RPE Cell and the Immune System." In Retinal Pigment Epithelium in Health and Disease. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-28384-1_6.

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Hass, Daniel T., Kriti Pandey, Brian M. Robbings, Rayne Lim, Jennifer R. Chao, and James B. Hurley. "Less Is More: The RPE Cell Culture Medium Additive THT Mildly Impairs RPE Health." In Advances in Experimental Medicine and Biology. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-76550-6_55.

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Ben M’Barek, Karim, Walter Habeler, Florian Regent, and Christelle Monville. "Developing Cell-Based Therapies for RPE-Associated Degenerative Eye Diseases." In Pluripotent Stem Cells in Eye Disease Therapy. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-28471-8_3.

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Hazim, Roni A., and David S. Williams. "The Importance of Differentiated RPE Cultures for studying Cell Biological Processes." In Advances in Experimental Medicine and Biology. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-76550-6_58.

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Conference papers on the topic "RPE in-Cell"

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Gulbrandsen, Egil, Jon Kvarekvål, and Harald Miland. "Effect of Oxygen Contamination on the Inhibition of CO2 Corrosion." In CORROSION 2001. NACE International, 2001. https://doi.org/10.5006/c2001-01054.

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Abstract The effect of dissolved O2 on the inhibition of CO2 corrosion was studied using carbon steel rotating cylinder electrodes (RCE). The test conditions were 40 °C, 1 bar CO2, pH 5.5, 1 wt% NaCl solution. The uninhibited CO2 corrosion rate was ca. 2 mm/y under these conditions. Three organic, film-forming corrosion inhibitor formulations were tested in the concentration range 20-50 ppm. With less than 1 ppb of dissolved O2 in the test brine, the inhibitors reduced the corrosion rate by two orders of magnitude, and the inhibited corrosion rates were insensitive to RCE rotation rate (100-50
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Alota, S., N. Azzerri, R. Bruno, et al. "Multilayer Electrogalvanizing of Steel Strip at High Current Density: Deposition Mechanisms, Coating Morphology and Corrosion Behaviour." In CORROSION 1985. NACE International, 1985. https://doi.org/10.5006/c1985-85386.

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Abstract High current density electrodeposition mechanisms of monolayer and multilayer coatings of Zn and Zn/Cr/CrOx were investigated. The effects of plating bath chemistry, temperature, current density, relative velocity between strip and electrolyte were examined in the laboratory by means of simulation systems involving the use of RCE, RDE and canal-shaped cell. Dependence between current density and Reynolds number resulting in an optimum structure and a macroscopically flat compact, silver-coloured coating with high corrosion resistance was evaluated. High current density electrogalvaniz
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Hinds, Gareth. "On the Choice of Applied Potential in Ex Situ Testing of Bipolar Plate Materials." In CONFERENCE 2023. AMPP, 2023. https://doi.org/10.5006/c2023-19547.

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Abstract Ex situ testing of candidate bipolar plate materials for polymer electrolyte membrane (PEM) fuel cells and electrolyzers typically involves electrochemical polarization of the specimen in a three electrode cell. Relatively high potentials of between 1.5 V and 2.0 V vs RHE are commonly applied during such tests due to the widely held assumption that, during both start-up/shutdown and normal operation, the bipolar plate experiences the same potential as that of the nearest electrode. Here we present experimental and modelling evidence that the bipolar plate in an operating PEM fuel cell
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Al-Hashem, A., J. Carew, and A. Al-Borno. "The Synergistic Effect of Corrosion, Scale, and Biocide Inhibitors on the Corrosion Rate of L-80 Steel in Seawater." In CORROSION 2003. NACE International, 2003. https://doi.org/10.5006/c2003-03241.

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Abstract The corrosion rate of L-80 steel in seawater was investigated in the presence of corrosion, scale, and biocide inhibitors separately and in combination of each. Experiments were conducted using the rotating cylinder electrode method under autoclave conditions with rotational speeds of 1000 and 2000 rpm. The temperature of the autoclave was maintained at 80 C, and CO2 was purged into the cell for 3 h. The corrosion rate of L-80 was determined by the LPR and gravimetric methods. Results indicated that the combination of scale-corrosion inhibitors and biocide-scale-corrosion inhibitors p
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Lee, Kun-Lin John, and Srdjan Nesic. "EIS Investigation of CO2/H2S Corrosion." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04728.

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Abstract This paper covers one of the building blocks required to complete a mechanistic CO2 / H2S corrosion model: electrochemistry of carbon steel CO2 corrosion in the presence of traces of H2S (3-250 ppm in the gas phase). Electrochemical impedance spectroscopy and linear polarisation resistance were used to investigate the mechanism of CO2/H2S corrosion in a glass cell at room temperature, at atmospheric pressure, pH5, using a rotating cylinder electrode at 1000 rpm. Very thin sulphide film was formed on the steel electrode in all experiments with H2S. At low concentrations (&amp;lt;15 ppm
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Cho, Bovinille Anye, George Mbella Teke, Godfrey K. Gakingo, Robert W. M. Pott, and Dongda Zhang. "CFD Simulations of Mixing Dynamics and Photobioreaction Kinetics in Miniature Bioreactors under Transitional Flow Regimes." In The 35th European Symposium on Computer Aided Process Engineering. PSE Press, 2025. https://doi.org/10.69997/sct.197756.

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Miniaturised stirred bioreactors are crucial in high-throughput bioprocesses for their simplicity and cost-effectiveness. To accelerate process optimisation in chemical and bioprocess industries, models that integrate CFD-predicted flow fields with (bio)reaction kinetics are needed. However, conventional two-step coupling methods, which freeze flow fields after solving hydrodynamics and then address (bio)reaction transport, face numerical challenges in miniaturised systems due to unsteady radial flows, recirculation zones, and secondary vortices. These flow fluctuations prevent steady-state hy
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William Villamizar, S., Jose M. Lizcano, and B. Miguel Mateus. "Effect of Chromium on Corrosion Resistance of Oil Tube Steels in a Carbon Dioxide Corrosion System." In LatinCORR 2023. AMPP, 2023. https://doi.org/10.5006/lac23-20972.

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Abstract The corrosion behavior of steels with different chromium contents, N80, L80, P110, L80-1%Cr, L80-3%Cr and L80-13%Cr in formation water containing CO2 was investigated by means of electrochemical measurements and tests of weight loss in an autoclave at HTHP. The characteristics of corrosion scale were characterized by X-ray diffraction. Effects of chromium concentration on the corrosion behavior of these carbon steel were discussed. The results of the electrochemical tests for both resistance to linear polarization and potentiodynamic polarization in a glass cell at 50 °C, atmospheric
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Nesic, Srdjan, Nicolas Thevenot, Jean-Louis Crolet, and Dragutin M. Drazic. "Electrochemical Properties of Iron Dissolution in the Presence of CO2 - Basics Revisited." In CORROSION 1996. NACE International, 1996. https://doi.org/10.5006/c1996-96003.

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Abstract In order to shed more light on the anodic reaction mechanism in CO2 corrosion of mild steel, two different kinds of electrochemical measurements were used: potentiodynamic sweep and galvanostatic measurements. Experiments were conducted in a glass cell at room temperature (T=22 ± 1 °C), different CO2 partial pressures (0 - 1 bar), over a broad pH range (2-7) using a rotating cylinder at 4000 rpm. Distinct and different anodic mechanisms were observed for pH&amp;lt;4 and for pH&amp;gt;5. In the intermediate area there seems to be a transition from one mechanism to another. New orders o
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Mohammed Nor, A., M. F. Suhor, M. F. Mohamed, M. Singer, and S. Nesic. "Corrosion of Carbon Steel in High CO2 Containing Environments - the Effect of High Flow Rate." In CORROSION 2012. NACE International, 2012. https://doi.org/10.5006/c2012-01683.

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Abstract The development of hydrocarbon gas fields which contain very high CO2 contents (up to 80 mol%) using carbon steel seems a difficult proposal and calls for the use of expensive corrosion resistant alloys. This would have the potential to render project development costs untenable. Unlike transportation and sequestration of supercritical CO2, where the amount of water is normally very small or negligible, gas field development has to consider the presence of formation water. This water can contain a high concentration of corrosive species, generated by large amounts of dissolved CO2. Th
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Delori, François C. "Fluorophotometer for Noninvasive Measurement of RPE Lipofuscin." In Noninvasive Assessment of the Visual System. Optica Publishing Group, 1992. http://dx.doi.org/10.1364/navs.1992.tuc3.

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Lipofuscin is a fluorescent pigment that accumulates throughout life preferentially in the macular RPE. At old age, lipofuscin is the major constituent of the cell as it occupies most of the free cytoplasmic [1]. It has been hypothesized that cell congestion by lipofuscin affects the normal metabolic activity of the RPE [1-3], and that this process plays a role in the pathogenesis of age-related macular degeneration (AMD). Massively engorged RPE cells are found adjacent to areas where the RPE is absent secondary to atrophy in AMD [3]. Although the evidence for lipofuscin involvement in AMD is
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Reports on the topic "RPE in-Cell"

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Carpita, Nicholas C., Ruth Ben-Arie, and Amnon Lers. Pectin Cross-Linking Dynamics and Wall Softening during Fruit Ripening. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7585197.bard.

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Our study was designed to elucidate the chemical determinants of pectin cross-linking in developing fruits of apple and peach and to evaluate the role of breakage cross-linkages in swelling, softening, and cell separation during the ripening. Peaches cell walls soften and swell considerably during the ripening, whereas apples fruit cells maintain wall firmness but cells separate during late stages of ripening. We used a "double-reduction" technique to show that levels of non-methyl esters of polyuronic acid molecules were constant during the development and ripening and decreased only in overr
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Manulis, Shulamit, Christine D. Smart, Isaac Barash, Guido Sessa, and Harvey C. Hoch. Molecular Interactions of Clavibacter michiganensis subsp. michiganensis with Tomato. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7697113.bard.

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Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial wilt and canker of tomato, is the most destructive bacterial disease of tomato causing substantial economic losses in Israel, the U.S.A. and worldwide. The molecular strategies that allow Cmm, a Gram-positive bacterium, to develop a successful infection in tomato plants are largely unknown. The goal of the project was to elucidate the molecular interactions between Cmmand tomato. The first objective was to analyze gene expression profiles of susceptible tomato plants infected with pathogenic and endophytic Cmms
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Tumwasorn, Somying, and Versalovic, James. Identification of probiotic lactobacillus with anti-inflammatory activity in the gastrointestinal tract. Chulalongkorn University, 2009. https://doi.org/10.58837/chula.res.2009.15.

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In order to identify probiotic Lactobacillus for potential use as immunoprobiotics for the amelioration or treatment of inflammatory diseases in the gastrointestinal tract such as Helicobacter pylori-induced gastro-duodenal diseases and Clostridium difficile-associated disease (CDAD), we chose to test for the ability of human-derived Lactobacillus to modulate interleukin-8 (IL8) production in gastrointestinal epithelial cells. Eighty-seven gastric biopsy-derived Lactobacillus isolates were tested for their ability to modulate IL-8 production in H.pylori-stimulated AGS cells and 37 feces-derive
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Prusky, Dov, Noel T. Keen, and Stanley Freeman. Elicitation of Preformed Antifungal Compounds by Non-Pathogenic Fungus Mutants and their Use for the Prevention of Postharvest Decay in Avocado Fruits. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7570573.bard.

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C. gloeosporioides attacks unripe avocado fruits in the orchard. Germinated spores produce appressoria that germinate and breach the cuticle, but the resultant subcuticular hyphae become quiescent and do not develop further until fruit is harvested and ripens. Resistance of unripe avocado to attach by C. gloeosporioides is correlated with the presence of fungitoxic concentrations of the preformed antifungal compound, 1-acetoxy-2-hydroxy-4-oxoheneicosa-12, 15 diene in the pericarp of unripe fruits. The objective of this proposal was to study the signal transduction process by which elicitors in
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Thanyasrisung, Panida, Aemvika Vittayaprasit, Voravee Hoven, Sugai, Motoyuki, and Oranart Matangkasombut. Rapid detection of mutans streptococci by substrate specific binding of automutanolysin : Final report. Faculty of Dentistry, Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.19.

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Chair-side rapid detection of mutans streptococci is an important aid to clinical dental caries risk assessment. Rapid Streptococcus mutans detection tools are available on the market but there are a small number. Automutanolysin (Aml) is a peptidoglycan hydrolase whose cell wall-binding domain (CWBD) has substrate-specificity towards mutans streptococci. This study aims to develop a rapid detection assay using CWBD conjugated with horseradish peroxidase (HRP). However, the recombinant protein was as insoluble form. Therefore, magnetic nanoparticles were used as an alternative reporter to conj
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Friedmann, Michael, Charles J. Arntzen, and Hugh S. Mason. Expression of ETEC Enterotoxin in Tomato Fruit and Development of a Prototype Transgenic Tomato for Dissemination as an Oral Vaccine in Developing Countries. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7585203.bard.

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The broad objective of the project was to develop a feasible approach to combat diarrheal disease caused by ETEC through the development of a low-cost oral immunogen in tomato fruit, expressed in the context of a prototype tomato that would answer the shortcomings of plant oral vaccines, especially in terms of produce handling and control of gene escape. Specifically, the goals for Boyce Thompson Institute (BTI) on this project were to develop transgenic tomato lines that express the enterotoxigenic E. coli (ETEC) heat-labile enterotoxin (LT) subunits A and/or B for use in oral edible vaccines
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Sengupta-Gopalan, Champa, Shmuel Galili, and Rachel Amir. Improving Methionine Content in Transgenic Forage Legumes. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7580671.bard.

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Leguminous forage crops are high in proteins but deficient in S- amino acids. It has been shown that both wool quality and milk production can be limited by the post-ruminal supply of sulfur-containing amino acids. Efforts to use conventional plant breeding and cell selection techniques to increase the S-amino acid content of alfalfa have met with little success. With the objective to increase the S-amino acid content of forage legumes, the goal of this project was to co- express the methionine rich zein genes from corn along with a gene for a key enzyme in methionine biosynthesis, aspartate k
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Fahima, Tzion, and Jorge Dubcovsky. Map-based cloning of the novel stripe rust resistance gene YrG303 and its use to engineer 1B chromosome with multiple beneficial traits. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7598147.bard.

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Research problem: Bread wheat (Triticumaestivum) provides approximately 20% of the calories and proteins consumed by humankind. As the world population continues to increase, it is necessary to improve wheat yields, increase grain quality, and minimize the losses produced by biotic and abiotic stresses. Stripe rust, caused by Pucciniastriiformisf. sp. tritici(Pst), is one of the most destructive diseases of wheat. The new pathogen races are more virulent and aggressive than previous ones and have produced large economic losses. A rich source for stripe-rust resistance genes (Yr) was found in w
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Hovav, Ran, Peggy Ozias-Akins, and Scott A. Jackson. The genetics of pod-filling in peanut under water-limiting conditions. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597923.bard.

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Pod-filling, an important yield-determining stage is strongly influenced by water stress. This is particularly true for peanut (Arachishypogaea), wherein pods are developed underground and are directly affected by the water condition. Pod-filling in peanut has a significant genetic component as well, since genotypes are considerably varied in their pod-fill (PF) and seed-fill (SF) potential. The goals of this research were to: Examine the effects of genotype, irrigation, and genotype X irrigation on PF and SF. Detect global changes in mRNA and metabolites levels that accompany PF and SF. Explo
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