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Journal articles on the topic 'Rpf2'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 February 2022

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1

Feng, Chunda, James C. Correll, Katherine E. Kammeijer, and Steven T. Koike. "Identification of New Races and Deviating Strains of the Spinach Downy Mildew Pathogen Peronospora farinosa f. sp. spinaciae." Plant Disease 98, no. 1 (2014): 145–52. http://dx.doi.org/10.1094/pdis-04-13-0435-re.

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Spinach downy mildew disease, caused by the obligate pathogen Peronospora farinosa f. sp. spinaciae, is the most economically important spinach (Spinacia oleracea) disease. New races of this pathogen have been emerging at a rapid rate over the last 15 years. This is likely due to production changes, particularly in California, such as high-density plantings and year-round spinach production. As of 2004, 10 races of P. farinosa f. sp. spinaciae had been identified, and the spinach resistance locus RPF2 provided resistance to races 1 to 10. Based on disease reactions on a set of spinach differen
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Asano, Nozomi, Akiyoshi Nakamura, Keisuke Komoda, Koji Kato, Isao Tanaka, and Min Yao. "Crystallization and preliminary X-ray crystallographic analysis of ribosome assembly factors: the Rpf2–Rrs1 complex." Acta Crystallographica Section F Structural Biology Communications 70, no. 12 (2014): 1649–52. http://dx.doi.org/10.1107/s2053230x14024182.

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Rpf2 and Rrs1 are essential proteins for ribosome biogenesis. These proteins form a complex (the Rpf2-subcomplex) with 5S rRNA and two ribosomal proteins (L5 and L11). This complex is recruited to the ribosome precursor (the 90S pre-ribosome). This recruitment is necessary for the maturation of 25S rRNA. Genetic depletion of Rpf2 and Rrs1 results in accumulation of the 25S rRNA precursor. In this study, Rpf2 and Rrs1 fromAspergillus nidulanswere co-overexpressed inEscherichia coli, purified and crystallized. Subsequent analysis revealed that these crystals contained the central core region of
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3

Guo, Yinping, Yanping Zhang, Jian-Liang Li, and Nian Wang. "Diffusible Signal Factor-Mediated Quorum Sensing Plays a Central Role in Coordinating Gene Expression of Xanthomonas citri subsp. citri." Molecular Plant-Microbe Interactions® 25, no. 2 (2012): 165–79. http://dx.doi.org/10.1094/mpmi-07-11-0184.

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Diffusible signal factor (DSF) family signal-mediated quorum sensing (QS) has been identified in many gram-negative bacteria. This QS pathway of Xanthomonas spp. consists of three major QS components: RpfF, RpfC, and RpfG. The rpfF gene encodes a putative enoyl-CoA hydratase that catalyzes the synthesis of the signal molecule. RpfC and RpfG serve as a two-component system for the perception and transduction of the extracellular DSF family signals. In order to further characterize the QS regulatory network in Xanthomonas citri subsp. citri, we investigated the RpfF, RpfC, and RpfG regulons by u
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Feng, Chunda, Katsunori Saito, Bo Liu, et al. "New Races and Novel Strains of the Spinach Downy Mildew Pathogen Peronospora effusa." Plant Disease 102, no. 3 (2018): 613–18. http://dx.doi.org/10.1094/pdis-05-17-0781-re.

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Downy mildew disease, caused by Peronospora effusa (=P. farinosa f. sp. spinaciae [Pfs]), is the most economically important disease of spinach. Current high-density fresh-market spinach production provides conducive conditions for disease development, and downy mildew frequently forces growers to harvest early owing to disease development, to cull symptomatic leaves prior to harvest, or to abandon the field if the disease is too severe. The use of resistant cultivars to manage downy mildew, particularly on increasing acreages of organic spinach production, applies strong selection pressure on
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5

Haymes, K. M., S. C. Hokanson, K. Salazar, and J. Maas. "Molecular Markers Linked to Phytophthora fragariae Resistance Genes in Strawberry." HortScience 33, no. 3 (1998): 500a—500. http://dx.doi.org/10.21273/hortsci.33.3.500a.

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The commercial strawberry (Fragaria × ananassa Duch.) is susceptible to red stele root rot caused by the fungus Phytophthora fragariae Hickman var. fragariae. Characteristics of the disease are the reddened steles and “rat tail” appearance of the roots. Infected plants are dwarfed, exhibit wilting, and eventually die. Red stele resistance in strawberries and virulence of P. fragariae behave according to the gene-for-gene system. Resistance genes and their corresponding avirulence genes have been identified by screening plant roots for infection. The goal of this international research project
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6

Chauviac, Francois-Xavier, Giles Robertson, Doris H. X. Quay, et al. "The RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation-promoting factor catalytic domain." Acta Crystallographica Section F Structural Biology Communications 70, no. 8 (2014): 1022–26. http://dx.doi.org/10.1107/s2053230x1401317x.

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The first structure of the catalytic domain of RpfC (Rv1884), one of the resuscitation-promoting factors (RPFs) fromMycobacterium tuberculosis, is reported. The structure was solved using molecular replacement once the space group had been correctly identified as twinnedP21rather than the apparentC2221by searching for anomalous scattering sites inP1. The structure displays a very high degree of structural conservation with the previously published structures of the catalytic domains of RpfB (Rv1009) and RpfE (Rv2450). This structural conservation highlights the importance of the versatile doma
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7

Luk’yanchuk, I. V., A. S. Lyzhin, and I. I. Kozlova. "Analysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpfl genes with molecular markers." Vavilov Journal of Genetics and Breeding 22, no. 7 (2018): 795–99. http://dx.doi.org/10.18699/vj18.423.

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Strawberry (Fragaria x ananassa Duch.) varieties are susceptible to many fungal diseases. Identification of forms, carrying resistance genes, is an important stage in breeding programs leading to resistant varieties. The use of molecular markers allows to determine with high reliability the presence of the necessary genes in the genome and to identify promising forms. Some of the common strawberry's diseases, causing significant damage to strawberry plantations, are anthracnose (Colletotrichum acutatum Simmonds) and red stele root rot (Phytophthora fragariae var. fragariae Hickman). Dominant R
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8

Kharde, Satyavati, Fabiola R. Calviño, Andrea Gumiero, Klemens Wild, and Irmgard Sinning. "The structure of Rpf2–Rrs1 explains its role in ribosome biogenesis." Nucleic Acids Research 43, no. 14 (2015): 7083–95. http://dx.doi.org/10.1093/nar/gkv640.

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9

de Weg, W. E. Van. "Resistance to Phytophthora fragariae var. fragariae in strawberry: the Rpf2 gene." Theoretical and Applied Genetics 94, no. 8 (1997): 1092–96. http://dx.doi.org/10.1007/s001220050520.

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10

Asano, Nozomi, Koji Kato, Akiyoshi Nakamura, Keisuke Komoda, Isao Tanaka, and Min Yao. "Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis." Nucleic Acids Research 43, no. 9 (2015): 4746–57. http://dx.doi.org/10.1093/nar/gkv305.

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11

Wang, Xing-Yu, Lian Zhou, Jun Yang, Guang-Hai Ji, and Ya-Wen He. "The RpfB-Dependent Quorum Sensing Signal Turnover System Is Required for Adaptation and Virulence in Rice Bacterial Blight Pathogen Xanthomonas oryzae pv. oryzae." Molecular Plant-Microbe Interactions® 29, no. 3 (2016): 220–30. http://dx.doi.org/10.1094/mpmi-09-15-0206-r.

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Xanthomonas oryzae pv. oryzae, the bacterial blight pathogen of rice, produces diffusible signal factor (DSF) family quorum sensing signals to regulate virulence. The biosynthesis and perception of DSF family signals require components of the rpf (regulation of pathogenicity factors) cluster. In this study, we report that RpfB plays an essential role in DSF family signal turnover in X. oryzae pv. oryzae PXO99A. The production of DSF family signals was boosted by deletion of the rpfB gene and was abolished by its overexpression. The RpfC/RpfG-mediated DSF signaling system negatively regulates r
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12

Feng, Jia-Xun, Zhi-Zhong Song, Cheng-Jie Duan, et al. "The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice." Microbiology 155, no. 9 (2009): 3033–44. http://dx.doi.org/10.1099/mic.0.028910-0.

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Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice, one of the most serious rice diseases. The xrvA gene from Xoo strain 13751 encodes a protein containing a histone-like nucleoid-structuring protein (H-NS) domain. The expression of xrvA in strain 13751 was enhanced in XOM2 minimal medium. Mutation of the xrvA gene of strain 13751 led to a significant reduction in virulence in the host plant rice, a delayed hypersensitive response in the nonhost castor-oil plant, a decrease in extracellular polysaccharide and diffusible signal factor production, and an increase in intr
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13

Deng, Yinyue, Nadine Schmid, Chao Wang, et al. "Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnover." Proceedings of the National Academy of Sciences 109, no. 38 (2012): 15479–84. http://dx.doi.org/10.1073/pnas.1205037109.

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Many bacterial pathogens produce diffusible signal factor (DSF)-type quorum sensing (QS) signals in modulation of virulence and biofilm formation. Previous work on Xanthomonas campestris showed that the RpfC/RpfG two-component system is involved in sensing and responding to DSF signals, but little is known in other microorganisms. Here we show that in Burkholderia cenocepacia the DSF-family signal cis-2-dodecenoic acid (BDSF) negatively controls the intracellular cyclic dimeric guanosine monophosphate (c-di-GMP) level through a receptor protein RpfR, which contains Per/Arnt/Sim (PAS)-GGDEF-EAL
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14

Wang, Nian, Jian-Liang Li, and Steven E. Lindow. "RpfF-Dependent Regulon of Xylella fastidiosa." Phytopathology® 102, no. 11 (2012): 1045–53. http://dx.doi.org/10.1094/phyto-07-12-0146-r.

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Xylella fastidiosa regulates traits important to both virulence of grape as well as colonization of sharpshooter vectors via its production of a fatty acid signal molecule known as DSF whose production is dependent on rpfF. Although X. fastidiosa rpfF mutants exhibit increased virulence to plants, they are unable to be spread from plant to plant by insect vectors. To gain more insight into the traits that contribute to these processes, a whole-genome Agilent DNA microarray for this species was developed and used to determine the RpfF-dependent regulon by transcriptional profiling. In total, 44
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15

Zhang, J., P. Harnpicharnchai, J. Jakovljevic, et al. "Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes." Genes & Development 21, no. 20 (2007): 2580–92. http://dx.doi.org/10.1101/gad.1569307.

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16

Russell-Goldman, Eleanor, Jiayong Xu, Xiaobing Wang, John Chan, and JoAnn M. Tufariello. "A Mycobacterium tuberculosis Rpf Double-Knockout Strain Exhibits Profound Defects in Reactivation from Chronic Tuberculosis and Innate Immunity Phenotypes." Infection and Immunity 76, no. 9 (2008): 4269–81. http://dx.doi.org/10.1128/iai.01735-07.

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ABSTRACT Resuscitation-promoting factors (Rpfs), apparent peptidoglycan hydrolases, have been implicated in the reactivation of dormant bacteria. We previously demonstrated that deletion of rpfB impaired reactivation of Mycobacterium tuberculosis in a mouse model. Because M. tuberculosis encodes five Rpf paralogues, redundant functions among the family members might obscure rpf single-knockout phenotypes. A series of rpf double knockouts were therefore generated. One double mutant, ΔrpfAB, exhibited several striking phenotypes. Consistent with the proposed cell wall-modifying function of Rpfs,
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17

Dermawan, Doni. "A review on construction of pcDNA 3.1+ with cloning RpfB, RpfD, and RpfE genes of mycobacterium tuberculosis as DNA vaccine." American Journal of Obstetrics and Gynecology 217, no. 6 (2017): 727. http://dx.doi.org/10.1016/j.ajog.2017.08.065.

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18

O'Connell, Aileen, Shi-Qi An, Yvonne McCarthy, et al. "Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris." Molecular Plant-Microbe Interactions® 26, no. 10 (2013): 1131–37. http://dx.doi.org/10.1094/mpmi-05-13-0155-r.

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The black rot pathogen Xanthomonas campestris utilizes molecules of the diffusible signal factor (DSF) family as signals to regulate diverse processes contributing to virulence. DSF signal synthesis and transduction requires proteins encoded by the rpf gene cluster. RpfF catalyzes DSF synthesis, whereas the RpfCG two-component system links the perception of DSF to alteration in the level of the second messenger cyclic di-GMP. As this nucleotide can exert a regulatory influence at the post-transcriptional and post-translational levels, we have used comparative proteomics to identify Rpf-regulat
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19

Hartmann, Michael, Aiko Barsch, Karsten Niehaus, Alfred P�hler, Andreas Tauch, and J�rn Kalinowski. "The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum." Archives of Microbiology 182, no. 4 (2004): 299–312. http://dx.doi.org/10.1007/s00203-004-0713-1.

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20

Almeida, Rodrigo P. P., Nabil Killiny, Karyn L. Newman, Subhadeep Chatterjee, Michael Ionescu, and Steven E. Lindow. "Contribution of rpfB to Cell-to-Cell Signal Synthesis, Virulence, and Vector Transmission of Xylella fastidiosa." Molecular Plant-Microbe Interactions® 25, no. 4 (2012): 453–62. http://dx.doi.org/10.1094/mpmi-03-11-0074.

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In Xylella fastidiosa the fatty acid signal molecule diffusible signaling factor (DSF) is produced and sensed by components of the regulation of pathogenicity factors (rpf) cluster; lack of DSF production in RpfF mutants results in a non-vector-transmissible phenotype yet cells are hypervirulent to grape. rpfB has not been characterized in Xylella fastidiosa, although its homolog has been suggested to be required for DSF synthesis in Xanthomonas campestris pv. campestris. We show that RpfB is involved in DSF processing in both Xylella fastidiosa and Xanthomonas campestris, affecting the profil
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21

Sexton, Danielle L., Renée J. St-Onge, Henry J. Haiser, et al. "Resuscitation-Promoting Factors Are Cell Wall-Lytic Enzymes with Important Roles in the Germination and Growth of Streptomyces coelicolor." Journal of Bacteriology 197, no. 5 (2014): 848–60. http://dx.doi.org/10.1128/jb.02464-14.

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Dormancy is a common strategy adopted by bacterial cells as a means of surviving adverse environmental conditions. ForStreptomycesbacteria, this involves developing chains of dormant exospores that extend away from the colony surface. Both spore formation and subsequent spore germination are tightly controlled processes, and while significant progress has been made in understanding the underlying regulatory and enzymatic bases for these, there are still significant gaps in our understanding. One class of proteins with a potential role in spore-associated processes are the so-called resuscitati
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22

Choi, Ilyeong, Young Jeon, Youngki Yoo, Hyun-Soo Cho, and Hyun-Sook Pai. "The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis." Journal of Experimental Botany 71, no. 9 (2020): 2596–611. http://dx.doi.org/10.1093/jxb/eraa019.

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Abstract Yeast Rpf2 plays a critical role in the incorporation of 5S rRNA into pre-ribosomes by forming a binary complex with Rrs1. The protein characteristics and overexpression phenotypes of Arabidopsis Ribosome Production Factor 2 (ARPF2) and Arabidopsis Regulator of Ribosome Synthesis 1 (ARRS1) have been previously studied. Here, we analyze loss-of-function phenotypes of ARPF2 and ARRS1 using virus-induced gene silencing to determine their functions in pre-rRNA processing and ribosome biogenesis. ARPF2 silencing in Arabidopsis led to pleiotropic developmental defects. RNA gel blot analysis
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23

Mahne, Martina, Andreas Tauch, Alfred Pühler, and Jörn Kalinowski. "TheCorynebacterium glutamicumgenepmtencoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins." FEMS Microbiology Letters 259, no. 2 (2006): 226–33. http://dx.doi.org/10.1111/j.1574-6968.2006.00269.x.

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24

Huang, Tzu-Pi, and Amy C. Lee Wong. "A Cyclic AMP Receptor Protein-Regulated Cell-Cell Communication System Mediates Expression of a FecA Homologue in Stenotrophomonas maltophilia." Applied and Environmental Microbiology 73, no. 15 (2007): 5034–40. http://dx.doi.org/10.1128/aem.00366-07.

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ABSTRACT Stenotrophomonas maltophilia WR-C possesses an rpf/diffusible signal factor (DSF) cell-cell communication system. It produces cis-Δ2-11-methyl-dodecenoic acid, a DSF, and seven structural derivatives, which require rpfF and rpfB for synthesis. Acquisition of iron from the environment is important for bacterial growth as well as the expression of virulence genes. We identified a gene homologous to fecA, which encodes a ferric citrate receptor that transports exogenous siderophore ferric citrate from the environment into the bacterial periplasm. Western blot analysis with anti-FecA-His6
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25

Poplawsky, Alan R., Wesley Chun, Holly Slater, Michael J. Daniels, and J. Maxwell Dow. "Synthesis of Extracellular Polysaccharide, Extracellular Enzymes, and Xanthomonadin in Xanthomonas campestris: Evidence for the Involvement of Two Intercellular Regulatory Signals." Molecular Plant-Microbe Interactions® 11, no. 1 (1998): 68–70. http://dx.doi.org/10.1094/mpmi.1998.11.1.68.

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In previous work, the pigB transcriptional unit of Xanthomonas campestris was implicated in the generation of a diffusible factor (DF) that regulates xanthomonadin and extracellular polysaccharide (EPS) production. Similarly, the rpfF and rpfB genes were implicated in the generation of a diffusible factor (DSF) that regulates the production of extracellular enzymes and EPS. In this study, extracel-lular cross-feeding studies with rpfF and pigB mutant strains provided evidence for the existence of two separate intercellular signaling systems with overlapping roles in the regulation of EPS produ
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26

Jungwirth, Britta, Denise Emer, Iris Brune, et al. "Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR." FEMS Microbiology Letters 281, no. 2 (2008): 190–97. http://dx.doi.org/10.1111/j.1574-6968.2008.01098.x.

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27

Andreyeva, E. N., A. A. Ogienko, A. A. Yushkova, et al. "Non3 is an essential Drosophila gene required for proper nucleolus assembly." Vavilov Journal of Genetics and Breeding 23, no. 2 (2019): 190–98. http://dx.doi.org/10.18699/vj19.481.

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The nucleolus is a dynamic non-membrane-bound nuclear organelle, which plays key roles not only in ribosome biogenesis but also in many other cellular processes. Consistent with its multiple functions, the nucleolus has been implicated in many human diseases, including cancer and degenerative pathologies of the nervous system and heart. Here, we report the characterization of the Drosophila Non3 (Novel nucleolar protein 3) gene, which encodes a protein homologous to the human Brix domain-containing Rpf2 that has been shown to control ribosomal RNA (rRNA) processing. We used imprecise P-element
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28

Frederick, Reid D., Jiliang Chiu, Jeffrey L. Bennetzen, and Autar K. Handa. "Identification of a Pathogenicity Locus, rpfA, in Erwinia carotovora subsp. carotovora That Encodes a Two-Component Sensor-Regulator Protein." Molecular Plant-Microbe Interactions® 10, no. 3 (1997): 407–15. http://dx.doi.org/10.1094/mpmi.1997.10.3.407.

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A mutant of Erwinia carotovora subsp. carotovora, AH2552, created by a Mud1 insertion was found to be reduced in plant pathogenicity and deficient in extracellular protease and cellulase activity, although it produced normal levels of pectate lyase and polygalacturonase. A cosmid clone, pEC462, was isolated from a wild-type E. carotovora subsp. carotovora DNA library that concomitantly restored pathogenicity and protease and cellulase activities of AH2552 to wild-type levels when present in trans. The genetic locus that was disrupted in AH2552 by insertion of Mud1 has been designated rpfA, for
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29

Boligon, Arione Augusti, Victor Eduardo Sala, Maria Eugênia Zerlotti Mercadante, Enilson Geraldo Ribeiro, Joslaine Noely dos Santos Gonçalves Cyrillo, and Lucia Galvão de Albuquerque. "Parâmetros genéticos para diferentes relações de peso ao nascer e à desmama em vacas da raça Nelore." Ciência Rural 43, no. 4 (2013): 676–81. http://dx.doi.org/10.1590/s0103-84782013005000019.

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O presente estudo foi desenvolvido com o objetivo de estimar herdabilidade e repetibilidade para diferentes medidas de eficiência produtiva de vacas e determinar a melhor maneira de calcular as relações de peso, visando a sua utilização como critério de seleção em rebanhos da raça Nelore. Os dados analisados são de animais da raça Nelore, pertencentes ao Projeto de Seleção das Raças Zebuínas e Caracu do Centro APTA Bovinos de Corte, do Instituto de Zootecnia de Sertãozinho. Os animais são selecionados para maior peso ao sobreano (rebanhos NeS e NeT, analisados como um único rebanho) e para pes
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Waldron, Evan J., Daniel Snyder, Nicolas L. Fernandez, et al. "Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF." PLOS Biology 17, no. 2 (2019): e3000123. http://dx.doi.org/10.1371/journal.pbio.3000123.

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31

Beaudenon, Sylvie L., Maria R. Huacani, Guangli Wang, Donald P. McDonnell, and Jon M. Huibregtse. "Rsp5 Ubiquitin-Protein Ligase Mediates DNA Damage-Induced Degradation of the Large Subunit of RNA Polymerase II in Saccharomyces cerevisiae." Molecular and Cellular Biology 19, no. 10 (1999): 6972–79. http://dx.doi.org/10.1128/mcb.19.10.6972.

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ABSTRACT Rsp5 is an E3 ubiquitin-protein ligase of Saccharomyces cerevisiae that belongs to the hect domain family of E3 proteins. We have previously shown that Rsp5 binds and ubiquitinates the largest subunit of RNA polymerase II, Rpb1, in vitro. We show here that Rpb1 ubiquitination and degradation are induced in vivo by UV irradiation and by the UV-mimetic compound 4-nitroquinoline-1-oxide (4-NQO) and that a functional RSP5 gene product is required for this effect. The 26S proteasome is also required; a mutation ofSEN3/RPN2 (sen3-1), which encodes an essential regulatory subunit of the 26S
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32

Dow, J. Maxwell, Yvonne Fouhy, Jean F. Lucey, and Robert P. Ryan. "The HD-GYP Domain, Cyclic Di-GMP Signaling, and Bacterial Virulence to Plants." Molecular Plant-Microbe Interactions® 19, no. 12 (2006): 1378–84. http://dx.doi.org/10.1094/mpmi-19-1378.

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Cyclic di-GMP is an almost ubiquitous second messenger in bacteria that was first described as an allosteric activator of cellulose synthase but is now known to regulate a range of functions, including virulence in human and animal pathogens. Two protein domains, GGDEF and EAL, are implicated in the synthesis and degradation, respectively, of cyclic di-GMP. These domains are widely distributed in bacteria, including plant pathogens. The majority of proteins with GGDEF and EAL domains contain additional signal input domains, suggesting that their activities are responsive to environmental cues.
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Bi, Hongkai, Yonghong Yu, Huijuan Dong, Haihong Wang, and John E. Cronan. "Xanthomonas campestris RpfB is a fatty Acyl-CoA ligase required to counteract the thioesterase activity of the RpfF diffusible signal factor (DSF) synthase." Molecular Microbiology 93, no. 2 (2014): 262–75. http://dx.doi.org/10.1111/mmi.12657.

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Ren, Jinhai, Ying Wang, Lihua Wang, Xiaoling Guo, and Xiaonan Guo. "Ribophorin II is upregulated in myelodysplastic syndromes and prevents apoptosis and cell cycle progression." Experimental Biology and Medicine 245, no. 12 (2020): 1009–15. http://dx.doi.org/10.1177/1535370220927996.

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Myelodysplastic syndromes (MDSs) are a series of heterogeneous diseases affecting hematopoietic stem cells that result in hematopoiesis disturbance and leukemic transformation. As an essential cell cycle regulator, ribophorin II (RPN2) has been extensively identified as a prospective predictor of prognosis in diverse malignant tumors. However, its effects on MDS are unclear. We observed increased mRNA expression RPN2 in samples from MDS patients, compared with samples from normal healthy controls. RPN2 overexpression promoted the proliferation of Ontario Cancer Institute OCI-acute myeloid leuk
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Wang, C., C. Liu, G. W. Zhang, et al. "Effects of rumen-protected folic acid and betaine supplementation on growth performance, nutrient digestion, rumen fermentation and blood metabolites in Angus bulls." British Journal of Nutrition 123, no. 10 (2020): 1109–16. http://dx.doi.org/10.1017/s0007114520000331.

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AbstractThis study evaluated the effects of rumen-protected folic acid (RPFA) and betaine (BT) on growth performance, nutrient digestion and blood metabolites in bulls. Forty-eight Angus bulls were blocked by body weight and randomly assigned to four treatments in a 2 × 2 factorial design. BT of 0 or 0·6 g/kg DM was supplemented to diet without or with the addition of 6 mg/kg DM of folic acid from RPFA, respectively. Average daily gain increased by 25·2 and 6·29 % for addition of BT without RPFA and with RPFA, respectively. Digestibility and ruminal total volatile fatty acids of neutral-deterg
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36

Seo, Hak Soo. "A Series of Novel rps2 Alleles Disrupt the RPS2-avrRpt2 Gene-for-gene Interaction." Journal of the Korean Society for Applied Biological Chemistry 52, no. 3 (2009): 305–8. http://dx.doi.org/10.3839/jksabc.2009.054.

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37

Sukhodolets, Karen E., Alison B. Hickman, Sunita K. Agarwal, et al. "The 32-Kilodalton Subunit of Replication Protein A Interacts with Menin, the Product of the MEN1 Tumor Suppressor Gene." Molecular and Cellular Biology 23, no. 2 (2003): 493–509. http://dx.doi.org/10.1128/mcb.23.2.493-509.2003.

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ABSTRACT Menin is a 70-kDa protein encoded by MEN1, the tumor suppressor gene disrupted in multiple endocrine neoplasia type 1. In a yeast two-hybrid system based on reconstitution of Ras signaling, menin was found to interact with the 32-kDa subunit (RPA2) of replication protein A (RPA), a heterotrimeric protein required for DNA replication, recombination, and repair. The menin-RPA2 interaction was confirmed in a conventional yeast two-hybrid system and by direct interaction between purified proteins. Menin-RPA2 binding was inhibited by a number of menin missense mutations found in individual
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38

WANG, C., Q. LIU, G. GUO, et al. "Effects of different dietary protein levels and rumen-protected folic acid on ruminal fermentation, degradability, bacterial populations and urinary excretion of purine derivatives in beef steers." Journal of Agricultural Science 155, no. 9 (2017): 1477–86. http://dx.doi.org/10.1017/s0021859617000533.

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SUMMARYThe current experiment was conducted to evaluate the effects of different dietary protein levels and rumen-protected folic acid (RPFA) supplementation on ruminal fermentation, microbial enzyme activity, bacterial populations and urinary excretion of purine derivatives (PD) in growing beef steers. Low-protein (LP) or high-protein (HP) diets were fed to eight ruminally cannulated first-generation cross-bred (Blonde d'Aquitaine × Simmental) beef steers with or without RPFA supplementation. Steers were fed a total mixed ration, and dietary concentrate to maize silage ratio was 50 : 50 (dry
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39

Malamud, Florencia, Pablo S. Torres, Roxana Roeschlin, et al. "The Xanthomonas axonopodis pv. citri flagellum is required for mature biofilm and canker development." Microbiology 157, no. 3 (2011): 819–29. http://dx.doi.org/10.1099/mic.0.044255-0.

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Xanthomonas axonopodis pv. citri (Xac) is the causative agent of citrus canker. This bacterium develops a characteristic biofilm on both biotic and abiotic surfaces. To evaluate the participation of the single flagellum of Xac in biofilm formation, mutants in the fliC (flagellin) and the flgE (hook) genes were generated. Swimming motility, assessed on 0.25 % agar plates, was markedly reduced in fliC and flgE mutants. However, the fliC and flgE mutants exhibited a flagellar-independent surface translocation on 0.5 % agar plates. Mutation of either the rpfF or the rpfC gene, which both encode pr
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40

Rothwell, Christopher T., Davinder Singh, Peter M. Dracatos, and Robert F. Park. "Inheritance and Characterization of Rph27: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn." Phytopathology® 110, no. 5 (2020): 1067–73. http://dx.doi.org/10.1094/phyto-12-19-0470-r.

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The barley cultivar Quinn was previously reported to carry two genes for resistance to Puccinia hordei, viz. Rph2 and Rph5. In this study, we characterized and mapped a third resistance gene (RphCRQ3) in cultivar Quinn. Multipathotype testing in the greenhouse on a panel of barley genotypes previously postulated to carry Rph2 revealed rare race specificity in four genotypes in response to P. hordei pathotype (pt.) 222 P+ (virulent on Rph2 and Rph5). This suggested either the presence of a race-specific allele variant of Rph2 or the presence of an independent uncharacterized leaf rust resistanc
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41

Song, Bo, Mengyun Jiang, and Lei Gao. "RiboNT: A Noise-Tolerant Predictor of Open Reading Frames from Ribosome-Protected Footprints." Life 11, no. 7 (2021): 701. http://dx.doi.org/10.3390/life11070701.

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Ribo-seq, also known as ribosome profiling, refers to the sequencing of ribosome-protected mRNA fragments (RPFs). This technique has greatly advanced our understanding of translation and facilitated the identification of novel open reading frames (ORFs) within untranslated regions or non-coding sequences as well as the identification of non-canonical start codons. However, the widespread application of Ribo-seq has been hindered because obtaining periodic RPFs requires a highly optimized protocol, which may be difficult to achieve, particularly in non-model organisms. Furthermore, the periodic
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42

Morris, Michael J., Tomasz M. Beer, Yohann Loriot, et al. "Correlation between radiographic progression-free survival (rPFS) and overall survival (OS): Results from PREVAIL." Journal of Clinical Oncology 34, no. 2_suppl (2016): 182. http://dx.doi.org/10.1200/jco.2016.34.2_suppl.182.

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182 Background: In PREVAIL, enzalutamide (ENZA) significantly improved OS (hazard ratio [HR] 0.71; P < 0.0001) and rPFS (HR 0.19; P < 0.0001) compared with placebo in chemotherapy-naïve metastatic castration-resistant prostate cancer (mCRPC). Using data from the final analysis at 439 rPFS events we showed a strong correlation between investigator and central review. Here we report sensitivity analyses (SAs) from the final analysis of investigator-assessed rPFS and the association between investigator-assessed rPFS and OS. Methods: The coprimary endpoint of rPFS was defined as time from r
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43

Xiao, Wei, Arindam Dey, and Le Hoang Son. "A study on regular picture fuzzy graph with applications in communication networks." Journal of Intelligent & Fuzzy Systems 39, no. 3 (2020): 3633–45. http://dx.doi.org/10.3233/jifs-191913.

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Picture fuzzy graph (PFG) is an extended version of intuitionistic fuzzy graph (IFG) to model the uncertain real world problems, in which IFG may fail to model those problems properly. PFG is more precise, flexible and compatible than IFG to deal the real-life scenarios which consists of information these types: yes, abstain, no and refusal. The main focus of our study is to present the concept of isomorphic PFG, regular PFG (RPFG) and picture fuzzy multigraph. In this paper, we present the notation of RPFG. Many different types of RPFGs such as regular strong PFG, regular complete PFG, comple
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Korutla, Laxminarayana N., Gwendolyn J. Stewart, Elizabeth C. Lasz, Theodore E. Maione, and Stefan Niewiarowski. "Evaluation of Recombinant Platelet Factor 4 and Protamine Sulfate for Heparin Neutralization: Clotting and Clearance Studies in Rat." Thrombosis and Haemostasis 71, no. 05 (1994): 609–14. http://dx.doi.org/10.1055/s-0038-1642491.

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SummaryRecombinant platelet factor 4 (rPF4) efficiently neutralized heparin anticoagulant activity in rats without the adverse effect of protamine sulfate (PS) (Circulation 1992; 85: 1102). This study confirmed that rPF4 and PS neutralized heparin in rats. In vitro addition of excess PS but not rPF4 to plasma prolonged the activated partial thromboplastin time. Injection of rPF4 or PS 2 min following injection of 3H-heparin augmented loss of radioactivity from the circulation over the first 2 min but did not affect the half life of 3H-heparin for the next 58 min. PS was coupled to 4-(p-Azidosa
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45

Martin, C., S. Okamura, and R. Young. "Genetic exploration of interactive domains in RNA polymerase II subunits." Molecular and Cellular Biology 10, no. 5 (1990): 1908–14. http://dx.doi.org/10.1128/mcb.10.5.1908.

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The two large subunits of RNA polymerase II, RPB1 and RPB2, contain regions of extensive homology to the two large subunits of Escherichia coli RNA polymerase. These homologous regions may represent separate protein domains with unique functions. We investigated whether suppressor genetics could provide evidence for interactions between specific segments of RPB1 and RPB2 in Saccharomyces cerevisiae. A plasmid shuffle method was used to screen thoroughly for mutations in RPB2 that suppress a temperature-sensitive mutation, rpb1-1, which is located in region H of RPB1. All six RPB2 mutations tha
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Martin, C., S. Okamura, and R. Young. "Genetic exploration of interactive domains in RNA polymerase II subunits." Molecular and Cellular Biology 10, no. 5 (1990): 1908–14. http://dx.doi.org/10.1128/mcb.10.5.1908-1914.1990.

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The two large subunits of RNA polymerase II, RPB1 and RPB2, contain regions of extensive homology to the two large subunits of Escherichia coli RNA polymerase. These homologous regions may represent separate protein domains with unique functions. We investigated whether suppressor genetics could provide evidence for interactions between specific segments of RPB1 and RPB2 in Saccharomyces cerevisiae. A plasmid shuffle method was used to screen thoroughly for mutations in RPB2 that suppress a temperature-sensitive mutation, rpb1-1, which is located in region H of RPB1. All six RPB2 mutations tha
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47

EL-SAYED, AYMAN. "A NEW APPROACH FOR CENTRALIZED END-SYSTEM MULTICAST PROTOCOL." International Journal of Information Acquisition 03, no. 01 (2006): 77–84. http://dx.doi.org/10.1142/s021987890600085x.

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In this paper we propose a new approach of application-level multicast protocol providing a group communication service. This protocol, called End-System Multicast (ESM), and can be used when native multicast routing is not available. ESM is a centralized protocol where everything is being controlled by a single host called Rendez-vous point (RPL1), connected indirectly to the group members via some hosts called secondary Rendez-vous Point (RPL2). Each RPL2 has some group members that constitute a cluster, and each cluster is controlled by its RPL2. Since the group control is divided among som
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48

Ryu, Young-Bok, Yang-Do Kim, Min-Su Kang, Gang-Woo Lee, Byung-Hyun Shon, and Man-Sig Lee. "The physicochemical characteristics of manufactured RPF by industrial combustibility waste." Journal of the Korea Academia-Industrial cooperation Society 11, no. 1 (2010): 234–41. http://dx.doi.org/10.5762/kais.2010.11.1.234.

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49

Pratama, Wahyu Agung, Andriansjah Rukmana, and Fithriyah Sjatha. "Ekspresi Protein Resuscitation promoting factor-D Mycobacterium tuberculosis pada sel CHO-K1 secara in-vitro." Jurnal Biotek Medisiana Indonesia 9, no. 1 (2020): 1–7. http://dx.doi.org/10.22435/jbmi.v9i1.3891.

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Abstrak Protein Resuscitation promoting factor–D (Rpf-D) merupakan protein transmembran dari Mycobacterium tuberculosis dan dieskpresikan pada fase reaktivasi infeksi laten menjadi aktif. Protein Rpf-D tersusun atas 154 asam amino berukuran 16 kDa yang dikode oleh 456 basa dari famili gen-Rpf. Pada studi sebelumnya, kami telah berhasil mengklona gen rpfD dari Mycobacterium tuberculosis strain Beijing kedalam plasmid pcDNA3.1, dikenal sebagai pcDNA3.1-rpfD. Dalam studi ini, kami mentransfeksi sel mamalia CHO-K1 dengan plasmid rekombinan pcDNA3.1-rpfD dan dilanjutkan dengan pewarnaan imunologis
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50

Halabi, Susan, Akash Roy, Qian Yang, Wanling Xie, William Kevin Kelly, and Christopher Sweeney. "Radiographic progression-free survival as a surrogate endpoint of overall survival in men with metastatic castrate-resistant prostate cancer." Journal of Clinical Oncology 39, no. 15_suppl (2021): 5057. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.5057.

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5057 Background: Radiographic progression-free survival (rPFS) is commonly used as a co-primary endpoint in randomized clinical trials in men with metastatic castrate-resistant prostate cancer (mCRPC). However, rPFS has not been established as a valid surrogate endpoint of overall survival (OS) in men with mCRPC. Here, we hypothesized that rPFS is a reliable surrogate for OS in mCRPC. We also explored whether PFS is a valid surrogate endpoint of OS at the aggregate trial level. Methods: We performed a systematic search of the literature encompassing the period January 2004-December 2020 using
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