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Journal articles on the topic 'RSC complex, Rsc2'

Author: Grafiati

Published: 9 March 2023

Last updated: 19 July 2025

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1

Bungard, David, Michelle Reed, and Edward Winter. "RSC1 and RSC2 Are Required for Expression of Mid-Late Sporulation-Specific Genes in Saccharomyces cerevisiae." Eukaryotic Cell 3, no. 4 (2004): 910–18. http://dx.doi.org/10.1128/ec.3.4.910-918.2004.

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ABSTRACT Rsc1 and Rsc2 are alternative bromodomain-containing subunits of the ATP-dependent RSC chromatin remodeling complex in Saccharomyces cerevisiae. Smk1 is a sporulation-specific mitogen-activated protein kinase homolog that is required for the postmeiotic events of spore formation. In this study we show that RSC1 and RSC2 are haploinsufficient for spore formation in a smk1 hypomorph. Moreover, diploids lacking Rsc1 or Rsc2 show a subset of smk1-like phenotypes. High-copy-number RSC1 plasmids do not suppress rsc2-Δ/rsc2-Δ sporulation defects, and high-copy-number RSC2 plasmids do not sup

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Wong, Michael C. V. L., Suzanna R. S. Scott-Drew, Matthew J. Hayes, Philip J. Howard та James A. H. Murray. "RSC2, Encoding a Component of the RSC Nucleosome Remodeling Complex, Is Essential for 2μm Plasmid Maintenance in Saccharomyces cerevisiae". Molecular and Cellular Biology 22, № 12 (2002): 4218–29. http://dx.doi.org/10.1128/mcb.22.12.4218-4229.2002.

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ABSTRACT The stable maintenance of the 2μm circle plasmid depends on its ability to overcome intrinsic maternal inheritance bias, which in yeast normally results in the failure to transmit DNA molecules efficiently to daughter cells. In addition to the plasmid proteins Rep1 and Rep2 acting on the plasmid DNA locus STB, it is likely that other chromosomally encoded yeast proteins are required. We have isolated mutants of yeast unable to maintain 2μm and found that RSC2 is essential for 2μm to overcome maternal inheritance bias. Rsc2 is part of a multisubunit RSC chromatin remodeling complex, an

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Baetz, Kristin K., Nevan J. Krogan, Andrew Emili, Jack Greenblatt, and Philip Hieter. "The ctf13-30/CTF13 Genomic Haploinsufficiency Modifier Screen Identifies the Yeast Chromatin Remodeling Complex RSC, Which Is Required for the Establishment of Sister Chromatid Cohesion." Molecular and Cellular Biology 24, no. 3 (2004): 1232–44. http://dx.doi.org/10.1128/mcb.24.3.1232-1244.2003.

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ABSTRACT The budding yeast centromere-kinetochore complex ensures high-fidelity chromosome segregation in mitosis and meiosis by mediating the attachment and movement of chromosomes along spindle microtubules. To identify new genes and pathways whose function impinges on chromosome transmission, we developed a genomic haploinsufficiency modifier screen and used ctf13-30, encoding a mutant core kinetochore protein, as the reference point. We demonstrate through a series of secondary screens that the genomic modifier screen is a successful method for identifying genes that encode nonessential pr

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4

Rossio, Valentina, Elena Galati, Matteo Ferrari, et al. "The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase." Journal of Cell Biology 191, no. 5 (2010): 981–97. http://dx.doi.org/10.1083/jcb.201007025.

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Upon prolonged activation of the spindle assembly checkpoint, cells escape from mitosis through a mechanism called adaptation or mitotic slippage, which is thought to underlie the resistance of cancer cells to antimitotic drugs. We show that, in budding yeast, this mechanism depends on known essential and nonessential regulators of mitotic exit, such as the Cdc14 early anaphase release (FEAR) pathway for the release of the Cdc14 phosphatase from the nucleolus in early anaphase. Moreover, the RSC (remodel the structure of chromatin) chromatin-remodeling complex bound to its accessory subunit Rs

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Hooshyar, Mohsen, Daniel Burnside, Maryam Hajikarimlou, et al. "Actin-Related Protein 6 (Arp6) Influences Double-Strand Break Repair in Yeast." Applied Microbiology 1, no. 2 (2021): 225–38. http://dx.doi.org/10.3390/applmicrobiol1020017.

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DNA double-strand breaks (DSBs) are the most deleterious form of DNA damage and are repaired through non-homologous end-joining (NHEJ) or homologous recombination (HR). Repair initiation, regulation and communication with signaling pathways require several histone-modifying and chromatin-remodeling complexes. In budding yeast, this involves three primary complexes: INO80-C, which is primarily associated with HR, SWR1-C, which promotes NHEJ, and RSC-C, which is involved in both pathways as well as the general DNA damage response. Here we identify ARP6 as a factor involved in DSB repair through

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Ma, Chien-Hui, Deepanshu Kumar, Makkuni Jayaram, Santanu K. Ghosh, and Vishwanath R. Iyer. "The selfish yeast plasmid exploits a SWI/SNF-type chromatin remodeling complex for hitchhiking on chromosomes and ensuring high-fidelity propagation." PLOS Genetics 19, no. 10 (2023): e1010986. http://dx.doi.org/10.1371/journal.pgen.1010986.

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Extra-chromosomal selfish DNA elements can evade the risk of being lost at every generation by behaving as chromosome appendages, thereby ensuring high fidelity segregation and stable persistence in host cell populations. The yeast 2-micron plasmid and episomes of the mammalian gammaherpes and papilloma viruses that tether to chromosomes and segregate by hitchhiking on them exemplify this strategy. We document for the first time the utilization of a SWI/SNF-type chromatin remodeling complex as a conduit for chromosome association by a selfish element. One principal mechanism for chromosome tet

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Titus, Laura C., T. Renee Dawson, Deborah J. Rexer, Kathryn J. Ryan, and Susan R. Wente. "Members of the RSC Chromatin-Remodeling Complex Are Required for Maintaining Proper Nuclear Envelope Structure and Pore Complex Localization." Molecular Biology of the Cell 21, no. 6 (2010): 1072–87. http://dx.doi.org/10.1091/mbc.e09-07-0615.

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The assembly, distribution, and functional integrity of nuclear pore complexes (NPCs) in the nuclear envelope (NE) are key determinants in the nuclear periphery architecture. However, the mechanisms controlling proper NPC and NE structure are not fully defined. We used two different genetic screening approaches to identify Saccharomyces cerevisiae mutants with defects in NPC localization. The first approach examined green fluorescent protein (GFP)-Nic96 in 531 strains from the yeast Tet-promoters Hughes Collection with individual essential genes expressed from a doxycycline-regulated promoter

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8

Shim, Eun Yong, Jia-Lin Ma, Ji-Hyun Oum, Yvonne Yanez, and Sang Eun Lee. "The Yeast Chromatin Remodeler RSC Complex Facilitates End Joining Repair of DNA Double-Strand Breaks." Molecular and Cellular Biology 25, no. 10 (2005): 3934–44. http://dx.doi.org/10.1128/mcb.25.10.3934-3944.2005.

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ABSTRACT Repair of chromosome double-strand breaks (DSBs) is central to cell survival and genome integrity. Nonhomologous end joining (NHEJ) is the major cellular repair pathway that eliminates chromosome DSBs. Here we report our genetic screen that identified Rsc8 and Rsc30, subunits of the Saccharomyces cerevisiae chromatin remodeling complex RSC, as novel NHEJ factors. Deletion of RSC30 gene or the C-terminal truncation of RSC8 impairs NHEJ of a chromosome DSB created by HO endonuclease in vivo. rsc30Δ maintains a robust level of homologous recombination and the damage-induced cell cycle ch

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9

Romeo, Martin J., Melinda L. Angus-Hill, Andrew K. Sobering, Yoshiaki Kamada, Bradley R. Cairns, and David E. Levin. "HTL1 Encodes a Novel Factor That Interacts with the RSC Chromatin Remodeling Complex in Saccharomyces cerevisiae." Molecular and Cellular Biology 22, no. 23 (2002): 8165–74. http://dx.doi.org/10.1128/mcb.22.23.8165-8174.2002.

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ABSTRACT RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for mutants that displayed additive growth defects with a conditional mutation in the protein kinase C gene (PKC1), which has been suggested through genetic connections to interact functionally with RSC. Several lines of evidence connect HTL1 to RSC function. First, an htl1Δ

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10

Soutourina, Julie, Véronique Bordas-Le Floch, Gabrielle Gendrel, et al. "Rsc4 Connects the Chromatin Remodeler RSC to RNA Polymerases." Molecular and Cellular Biology 26, no. 13 (2006): 4920–33. http://dx.doi.org/10.1128/mcb.00415-06.

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ABSTRACT RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol). Furthermore, the RSC complex coimmunoprecipitated with all three RNA polymerases. Mutations in the C terminus of Rsc4 conferred a thermosensitive phenotype and the loss of interaction with Rpb5. Certain thermosensitive rpb5 mutations were lethal in combination with an rsc4 mutation, supporting the physiological significance of the interaction. Pol II transcription of ca.

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11

Balachandra, Vinutha K., Jiyoti Verma, Madhu Shankar, et al. "The RSC (Remodels the Structure of Chromatin) complex of Candida albicans shows compositional divergence with distinct roles in regulating pathogenic traits." PLOS Genetics 16, no. 11 (2020): e1009071. http://dx.doi.org/10.1371/journal.pgen.1009071.

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Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. Biochemical analysis showed that CaRSC comprises 13 subunits and contains two novel non-essential members, which we named Nri1 and Nri2 (Novel RSC Interactors) that are exclusive to the CTG clade of Saccharomycotina. Genetic analysis showed distinct essentiality of C. albicans RSC subunits compared

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12

Kent, Nicholas A., Anna L. Chambers, and Jessica A. Downs. "Dual Chromatin Remodeling Roles for RSC during DNA Double Strand Break Induction and Repair at the Yeast MAT Locus." Journal of Biological Chemistry 282, no. 38 (2007): 27693–701. http://dx.doi.org/10.1074/jbc.m704707200.

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DNA double strand breaks (DSBs) are potentially serious chromosomal lesions. However, cells sometimes deliberately cleave their own DNA to facilitate certain chromosomal processes, and there is much interest in how such self-inflicted breaks are effectively managed. Eukaryotic DSBs occur in the context of chromatin and the RSC chromatin-remodeling ATPase complex has been shown to promote DSB repair at the budding yeast MAT locus DSB, created by the HO endonuclease during mating type switching. We show that the role of RSC at MAT is highly specialized. The Rsc1p subunit of RSC directs nucleosom

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13

Damelin, Marc, Itamar Simon, Terence I. Moy, et al. "The Genome-Wide Localization of Rsc9, a Component of the RSC Chromatin-Remodeling Complex, Changes in Response to Stress." Molecular Cell 9, no. 3 (2002): 563–73. http://dx.doi.org/10.1016/s1097-2765(02)00475-6.

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14

Sanz, Ana Belén, Sonia Díez-Muñiz, Jennifer Moya, et al. "Systematic Identification of Essential Genes Required for Yeast Cell Wall Integrity: Involvement of the RSC Remodelling Complex." Journal of Fungi 8, no. 7 (2022): 718. http://dx.doi.org/10.3390/jof8070718.

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Conditions altering the yeast cell wall lead to the activation of an adaptive transcriptional response mainly governed by the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Two high-throughput screenings were developed using the yTHC collection of yeast conditional mutant strains to systematically identify essential genes related to cell wall integrity, and those required for the transcriptional program elicited by cell wall stress. Depleted expression of 52 essential genes resulted in hypersensitivity to the dye Calcofluor white, with chromatin organization, Golgi

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15

Campsteijn, Coen, Anne-Marie J. Wijnands-Collin, and Colin Logie. "Reverse Genetic Analysis of Yeast RSC Chromatin Remodeling Complex Subunits Reveals a Role for RSC3 and SNF5 Homologue 1 in Ploidy Maintenance." PLoS Genetics preprint, no. 2007 (2005): e92. http://dx.doi.org/10.1371/journal.pgen.0030092.eor.

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16

Ljubotina, Predrag. "The influence of entrepreneurial skills, education and risk perception on career choice intent: The case of European students with family business background." Research in Social Change 12, no. 1 (2020): 23–37. http://dx.doi.org/10.2478/rsc-2020-0002.

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Abstract When confronted with career choice, students with family business background have a specific trilemma. Having an additional career alternative as a potential family business successor, makes their career choice decision more complex compared with their peers. The purpose of this paper is to investigate how entrepreneurial education, risk perception and skill mastering influence career choice intention of this specific group of students. We used a data set from GUESSS 2014 survey. Our sample includes students with family business background from 18 European countries. We used a multino

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17

Concas, Maria Pina, Anna Morgan, Paola Tesolin, Aurora Santin, Giorgia Girotto, and Paolo Gasparini. "Sensory Capacities and Eating Behavior: Intriguing Results from a Large Cohort of Italian Individuals." Foods 11, no. 5 (2022): 735. http://dx.doi.org/10.3390/foods11050735.

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Eating behavior (EB) is a complex system influenced by many factors, but an undisputed role is played by the senses. In this work, we examined the effect of the sensory capacities on EB in 1152 Italian adult individuals. After administering a questionnaire on EB and assessing sensory performance through standard audiometric, olfactory, and taste tests, the prevalence of reduced sensory capacities (RSCs) and the correlation with selected risk factors were calculated. Regression models, structural equation modelling, and conditional recursive partitioning were used to investigate the relationshi

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18

Bareiro, Diego, Enrique O’Durnin, Laura Oporto, and Christian Schaerer. "Shallow water model for pollutant distribution in the Ypacarai Lake." Revista de la Sociedad Científica del Paraguay 26, no. 2 (2021): 54–76. http://dx.doi.org/10.32480/rscp.2021.26.2.54.

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In this paper, we analyze the distribution of a non-reactive contaminant in Ypacarai Lake. We propose a shallow-water model that considers wind-induced currents, inflow and outflow conditions in the tributaries, and bottom effects due to the lakebed. The hydrodynamic is based on the depth-averaged Navier-Stokes equations considering wind stresses as force terms which are functions of the wind velocity. Bed (bottom) stress is based on Manning's equation, the lakebed characteristics, and wind velocities. The contaminant transportation is modeled by a 2D convection-diffusion equation taking into

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Andreto, Crislayne G., Mikhael Rodarte, Roseli M. Balestra, Lais S. Alves, Alexandre A. Ribeiro, and Magna Monteiro. "Biomimetic coating of alumina scaffolds at different immersion times." Revista de la Sociedad Científica del Paraguay 30, no. 1 (2025): 44–55. https://doi.org/10.32480/rscp.2025.30.1.4455.

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The biomimetic method can be used to coat scaffolds with calcium phosphate to improve bioactivity. This method presents the advantages of low cost, reproducibility, and applicability to complex surface and porous structures. This study aimed to manufacture alumina scaffolds coated with calcium phosphate by a biomimetic method for use as a biomaterial. The scaffolds were compacted at 200 MPa with 40 wt.% ammonium bicarbonate and 60 wt.% alumina, subjected to heat treatment at 270 °C for 120 min, and sintered at 1500 °C for 120 min. The samples were biomimetically coated by immersing them in a s

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Talajic, Mirko, Robert Kopal, and Leo Mršić. "Impact of Leadership on Business Performance – Leadership Analytical Concept." Research in Social Change 14, no. 1 (2022): 121–38. http://dx.doi.org/10.2478/rsc-2022-0009.

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Abstract Leaders have a difficult task as managing employees becomes more and more demanding and complex. Leadership mechanisms that were valid in the past are not valid today. Competition on the market is increasing and there is less and less room for differentiation. Leadership is an area in which organisations increasingly seek to differentiate themselves. However, to be able to do this it is necessary to understand all the complexity and breadth of leadership, the elements that need to be considered and how they affect each other. Many studies examine individual elements, but none offers a

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Ndreka, Alkida. "Return migration and re-integration of returnees challenges in the origin country." Research in Social Change 11, no. 3 (2019): 4–24. http://dx.doi.org/10.2478/rsc-2019-0012.

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Abstract Return migration, traditionally not a well-studied and often neglected area, is becoming an important component of the international migration debate. Reintegration is an essential part of return migration and identified as a complex process that is experienced differently by returnees. The adaptation of immigrants in the host country has been extensively studied, while much less attention has been paid to economic and socio-cultural reintegration and the difficulties return migrants face once they come back to their homeland. Especially children and youth born in destination countrie

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22

Yu, Feifei, Yuko Imamura, Masaru Ueno, et al. "The yeast chromatin remodeler Rsc1-RSC complex is required for transcriptional activation of autophagy-related genes and inhibition of the TORC1 pathway in response to nitrogen starvation." Biochemical and Biophysical Research Communications 464, no. 4 (2015): 1248–53. http://dx.doi.org/10.1016/j.bbrc.2015.07.114.

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23

Wang, Chunze, Rui Feng, Yao Chu, Qing Tan, Chaoyang Xing, and Fei Tang. "Simulations of the Rotor-Stator-Cavity Flow in Liquid-Floating Rotor Micro Gyroscope." Micromachines 14, no. 4 (2023): 793. http://dx.doi.org/10.3390/mi14040793.

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When rotating at a high speed in a microscale flow field in confined spaces, rotors are subject to a complex flow due to the joint effect of the centrifugal force, hindering of the stationary cavity and the scale effect. In this paper, a rotor-stator-cavity (RSC) microscale flow field simulation model of liquid-floating rotor micro gyroscopes is built, which can be used to study the flow characteristics of fluids in confined spaces with different Reynolds numbers (Re) and gap-to-diameter ratios. The Reynolds stress model (RSM) is applied to solve the Reynolds averaged Navier–Stokes equation fo

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24

Schlichter, Alisha, Margaret M. Kasten, Timothy J. Parnell, and Bradley R. Cairns. "Specialization of the chromatin remodeler RSC to mobilize partially-unwrapped nucleosomes." eLife 9 (June 4, 2020). http://dx.doi.org/10.7554/elife.58130.

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SWI/SNF-family chromatin remodeling complexes, such as S. cerevisiae RSC, slide and eject nucleosomes to regulate transcription. Within nucleosomes, stiff DNA sequences confer spontaneous partial unwrapping, prompting whether and how SWI/SNF-family remodelers are specialized to remodel partially-unwrapped nucleosomes. RSC1 and RSC2 are orthologs of mammalian PBRM1 (polybromo) which define two separate RSC sub-complexes. Remarkably, in vitro the Rsc1-containing complex remodels partially-unwrapped nucleosomes much better than does the Rsc2-containing complex. Moreover, a rsc1Δ mutation, but not

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25

Patel, Avinash B., Camille M. Moore, Basil J. Greber, et al. "Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement." eLife 8 (December 30, 2019). http://dx.doi.org/10.7554/elife.54449.

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Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric

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26

Navasa, Nicolás, Leandro Rodríguez-Aparicio, Miguel Ángel Ferrero, Andrea Monteagudo-Mera, and Honorina Martínez-Blanco. "Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB." Bioscience Reports 33, no. 3 (2013). http://dx.doi.org/10.1042/bsr20130018.

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We have shown previously that Escherichia coli K92 produces two different capsular polymers known as CA (colanic acid) and PA (polysialic acid) in a thermoregulated manner. The complex Rcs phosphorelay is largely related to the regulation of CA synthesis. Through deletion of rscA and rscB genes, we show that the Rcs system is involved in the regulation of both CA and PA synthesis in E. coli K92. Deletion of either rcsA or rcsB genes resulted in decreased expression of cps (CA biosynthesis cluster) at 19°C and 37°C, but only CA production was reduced at 19°C. Concerning PA, both deletions enhan

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27

Locci, Emanuela. "The Masonic Degree of Rose-Croix and Christianity: The Complex Links between Religion and Freemasonry during the Enlightenment." Ritual, Secrecy, and Civil Society, 2013. http://dx.doi.org/10.18278/rscs.1.2.2.

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Acoba, Michelle Grace, Ya‐Wen Lu, Selvaraju Kandasamy, et al. "Functional Insights on the Human Adenine Nucleotide Translocator Interactome." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.634.4.

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The adenine nucleotide translocator (ANT), by mediating the exchange of ADP for ATP across the inner mitochondrial membrane, lies at the core of oxidative phosphorylation (OXPHOS). Discrepancies in ANT expression and/or activity resulting to reduced energy metabolism are linked to a wide spectrum of pathologies, including lactic acidosis, progressive external opthalmoplegia, muscular dystrophy and cardiomyopathy. In yeast, the major isoform was modeled for a long time to exist in isolation, but was demonstrated to physically interact with respiratory supercomplexes (RSCs) exclusively in the co

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Yuan, Huiling, Ying Zhou, Yuping Lin, et al. "Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris." Biotechnology for Biofuels and Bioproducts 15, no. 1 (2022). http://dx.doi.org/10.1186/s13068-022-02150-w.

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Abstract Background Pichia pastoris is a widely used host organism for heterologous production of industrial proteins, such as cellulases. Although great progress has been achieved in improving protein expression in P. pastoris, the potential of the P. pastoris expression system has not been fully explored due to unknown genomic impact factors. Recently, whole-cell directed evolution, employing iterative rounds of genome-wide diversity generation and high-throughput screening (HTS), has been considered to be a promising strategy in strain improvement at the genome level. Results In this study,

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Professor, Peter L. Hays Former USAF Officer. "The Evolving United States Space – Enabled Reconnaissance – Strike Complex: Theory, Practice and Challenges." Air Force Review, no. 134 (April 14, 2025). https://doi.org/10.5281/zenodo.15163451.

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This article explains ways in which robust space capabilities have become an indispensable foundation for success in employing modern airpower. It considers how military space capabilities fit into conceptual models for modern warfare and uses these models to review evolution of the global reconnaissance-strike complex that first emerged in 1991 during Operation Desert Storm. It discusses specific ways space capabilities support each step in the find, fix, track, target, engage, and assess “kill chain” and outlines the many space systems the United States has deployed to modernize

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