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Lovelock, Laura Charlotte. "RsmN : a new atypical RsmA homologue in Pseudomonas aeruginosa." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12562/.
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The RsmA/CsrA family of global post-transcriptional regulators are small RNA-binding proteins involved in the regulation of a large number of genes such as those involved in quorum sensing, virulence factor production, secondary metabolism, motility and biofilm formation. They bind to target mRNAs and hence modulate their stability and translation rates. Their effects are antagonised by small non-coding regulatory RNAs. The control of expression of target genes via this post-transcriptional regulatory network is mostly operated in Pseudomonas spp. via the GacS/GacA two component system. This study aimed to perform a biophysical analysis of RsmA and to obtain a preliminary understanding of the structure, function and regulation of RsmN, a new atypical RsmA homologue from Pseudomonas aeruginosa. RsmA was purified and biophysical analysis confirmed that RsmA exists as a dimer and is highly stable at high temperatures (75 °C) and low pH (5.2). Although RsmN was found to be structurally similar to RsmA, no functional phenotypes have been identified. Consequently, rsmN was mutated and transcriptional fusions to rsmN and its anti-sense transcript were constructed for expression studies. Phenotypic analysis indicated that RsmN was not involved in the control of swarming, pyocyanin, elastase and protease production or glycogen accumulation. Unlike RsmA, RsmN does not have a control on the restriction modification system of P. aeruginosa. Transcriptional fusions revealed RetS, LadS and GacA all appear to have a 21 significant effect as activators of both the rsmN and nmsR promoters. 2-Alkyl-4(1H)-quinolone (AQ) signalling also modulate rsmN expression possibly via the iron chelating properties of 2-alkyl-3-hydroxy-4(1H)-heptyl-quinolone (PQS). RsmN targets identified from Deep Sequencing include those required for structural outer membrane proteins, transcriptional regulators as well as genes involved in motility, secretion, flagellar structure and biofilms. RsmA, RsmZ and RsmY were all identified as targets together with the small RNAs RgsA (indirectly gac-controlled) and the antagonistic RNA CrcZ (represses catabolite repression control protein Crc). Targets common to both RsmN and RsmA include the transcriptional regulators Vfr, PqsR, MvaT and Anr, regulatory RNAs RsmZ and RsmY together with transcripts corresponding to the pqsABCDE operon, LasB, LecA/B, RhlI, LasR/I, Crc and CrcZ. The identification of many mRNA targets for RsmN which are shared with targets of RsmA provides further evidence that RsmN is involved in global-post-transcriptional regulation of gene expression.
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Diaz, Manisha Regina. "Regulation of virulence gene expression by Rsm homologs in Pseudomonas aeruginosa." Diss., University of Iowa, 2014. https://ir.uiowa.edu/etd/4612.
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Pseudomonas aeruginosa RsmA belongs to the CsrA family of RNA binding proteins. CsrA family members are post-transcriptional regulators of global gene expression and usually function to inhibit translation of target genes, but in some cases can also exert positive regulatory effects. Previous work from our lab determined that RsmA is required for maximal T3SS gene expression in P. aeruginosa strain PA103. Nevertheless, the molecular mechanism underlying the RsmA-mediated control of T3SS gene expression was unknown. Expression of the T3SS is under the direct control of ExsA, a transcriptional activator. Previous microarray analyses showed that exsA transcript levels were reduced two-fold in an rsmA mutant. In chapter II I examine the role of RsmA in regulating ExsA expression. I demonstrate that expression of a ExsA-LacZ translational fusion was reduced two-fold in an rsmA mutant suggesting a specific effect of RsmA on ExsA expression. The effect of RsmA on ExsA expression occurs at a post-transcriptional level and is independent of mRNA and protein stabilization mechanisms. RsmA directly interacts with the exsCEBA transcript at multiple sites. Truncation analyses indicate that the -37 to +85 region (relative to the ATG start codon) is necessary and sufficient for RsmA-dependent control. I identified two binding sites, BS1 (-25 bp) and BS2 (+84), involved in the interaction of RsmA with the exsA transcript using sequence analysis, site-directed mutagenesis, EMSA assays, RNase footprints, and RNaseH cleavage assays. Mutagenesis of both binding sites results in an RsmA-independent phenotype. I further demonstrate that RsmA is able to activate ExsA expression. I propose a model wherein RsmA relieves a block on ExsA translation. Collectively, this work shows that RsmA directly binds and activates ExsA expression at the post-transcriptional level.
Most Pseudomonas species carry at least two homologs of CsrA on the chromosome, but only one copy had been identified in P. aeruginosa. Through the course of other projects in the lab, we observed several phenotypes that could not be accounted for by a single copy of RsmA. In collaboration with the Wolfgang lab, we identified a second CsrA homolog, RsmF in P. aeruginosa. RsmF is dimeric in solution. The structure of RsmF differs substantially from other CsrA homologs by having alpha-helices located between the beta-2 and beta-3 strands. In chapter III I examine the role of RsmF in regulating RsmA-controlled processes associated with acute (T3SS) and chronic (T6SS and biofilm formation) infection. I discovered that while an rsmF mutant alone does not exhibit a phenotype, simultaneous deletion of both rsmA and rsmF significantly accentuates the phenotypes exhibited by an rsmA mutant alone. I show that RsmA directly binds and represses RsmF translation and that the small regulatory RNAs RsmZ and RsmY do not significantly modulate RsmF activity. Site-directed mutagenesis revealed that Arg 62, located in the beta-1 and beta-5 fold, is essential for biological activity in vivo and RNA-binding in vitro suggesting a conserved mechanism of RNA recognition maintained across all CsrA family members. Finally, I show that RsmF binds to only a subset of RsmA targets and is not involved in the regulation of all RsmA-controlled processes. In chapter IV I identified high-affinity RNA ligands from a chemically synthesized oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX) and high-througput sequencing. From preliminary analyses of high-throughput sequencing data, the RsmF-binding consensus was determined as 5'-RUACARGGAC-3', with the ARGGA motif being 95% conserved. Collectively, this work shows that Rsm homologs play important roles in regulating virulence gene expression in P. aeruginosa.
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Gomes, Waldenor Ramone Juvito. "Solução analítica para o modelo RSOS." reponame:Repositório Institucional da UnB, 2016. http://repositorio.unb.br/handle/10482/21452.
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Dissertação (mestrado)—Universidade de Brasília, Instituto de Física, Programa de Pós-Graduação em Física, 2016.<br>Submitted by Fernanda Percia França (fernandafranca@bce.unb.br) on 2016-08-26T17:36:15Z
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2016_WaldenorRamoneJuvitoGomes.pdf: 761006 bytes, checksum: 85217e8d2f95a45f8d3451df3d06b44f (MD5)<br>Nesta dissertação iremos analisar a dinâmica do crescimento para modelos de autômatos celulares. Uma vez que a evolução cria uma superfície de fractal, mostraremos que a variação da rugosidade pode ser apresentada em uma expressão simples que combina duas componentes: a distribuição de probabilidade das configurações da superfície e as regras que define um autômato celular. A partir destas considerações investigaremos uma das variações do modelo RestrictedSolid-On-Solid(RSOS) em 1+1 dimensão e as propriedades da rugosidade, assim como as propriedades gerais que a mesma deve satisfazer. _________________________________________________________________________________________________ ABSTRACT<br>This dissertation will examine the growth dynamics for cellular automata models. Since the evolution creates a fractal surface, we show that the variations of roughness can be presented in simple expression that combine two combines: the probability distribution of the surface configurations and the rules that define a cellular automata. From these considerations we will investigate one of the variations of the Restricted Solid-On-Solid (RSOS) model in 1+1 dimensions and properties of the roughness, as well as the general properties that it must satisfy.
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Stacey, Sean D. "Regulating rsmA Expression in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etd/1232.
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Pseudomonas aeruginosa, a Gram-negative bacillus, commonly infects immunocompromised individuals and uses a variety of virulence factors to persist in these hosts. The posttranscriptional regulator, RsmA, plays a role in the expression of many virulence factors in P. aeruginosa. RsmA up regulates virulence factors used in colonizing hosts. However, regulation of rsmA is not well elucidated. Transposon mutagenesis was performed on P. aeruginosa containing a transcriptional rsmA-lacZ fusion to answer this question. Mutants were screened via β-galactosidase assay and transposon insertions identified via arbitrary PCR. A probable MFS transporter, we named mtpX, was one significant transposon mutant identified. A ΔmtpX mutant containing the rsmA-lacZ transcriptional fusion was constructed to confirm our results. Further analysis of rsmA, looking at RNA and protein levels, revealed varying results in nonmucoid versus mucoid backgrounds. Phenotypic assays were performed to characterize this unknown transporter and develop a putative mechanism as to how MtpX affects rsmA expression.
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González, Maturana Rodrigo. "Rodoviario San Francisco de la Selva RSFS." Tesis, Universidad de Chile, 2010. http://www.repositorio.uchile.cl/handle/2250/100311.
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Chen, Jui-Chun. "A virtual RSNS direction finding antenna system." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2004. http://library.nps.navy.mil/uhtbin/hyperion/04Dec%5FChen%5FJui.pdf.
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Thesis (M.S. in Electrical Engineering)--Naval Postgraduate School, Dec. 2004.<br>Thesis Advisor(s): David C. Jenn, Phillip E. Pace. Includes bibliographical references (p. 69-70). Also available online.
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Speaks, Tyler. "AlgR Directly Controls rsmA in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etd/2570.
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Pseudomonas aeruginosa is a bacterial pathogen that can infect any human tissue. The lungs of cystic fibrosis patients become chronically infected with Pseudomonas aeruginosa. Virulence factor gene expression is under elaborate regulatory control that remains poorly characterized. Understanding the regulatory hierarchy involved during infection is essential for identifying novel drug targets. RsmA is a post-transcriptional regulatory protein that controls expression of several virulence factors. Previous studies demonstrated alginate regulatory components AlgU and AlgR as regulators of rsmA expression. The aim of this study was to determine how AlgR controls rsmA expression. Western blot analysis of HA-tagged RsmA confirmed lower RsmA levels in an algR mutant. An electrophoretic mobility shift assay using purified AlgR demonstrated direct binding of AlgR to the rsmA promoter. These results indicate AlgR directly controls rsmA expression. We propose a mechanism whereby AlgR and AlgU work together to regulate rsmA.
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Androutsopoulos, Kalliopi. "Specification and verification of reactive systems with RSDS." Thesis, King's College London (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414509.
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Miller, Ian. "Post-transcriptional Regulation of RsmA In Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3481.
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Pseudomonas aeruginosa is a Gram-negative bacillus found in numerous environments. Gene regulatory mechanisms such as; Two-Component Systems, transcriptional and post-transcriptional regulators, and small non-coding RNAs control the expression of virulence factors that allow P. aeruginosa to initiate acute infections and persist as a chronic infection. A significant post-transcriptional regulator involved in these regulatory networks is the Regulator of Secondary Metabolites (RsmA). In this study, we investigated the contribution of a putative stem-loop on expression of RsmA. We constructed rsmA leader fusions to measure translation with and without the stem-loop present. Secondly, we introduced point mutations to disrupt the formation of the stem-loop. Finally, we performed Site-Directed Mutagenesis on the rsmA leader to examine protein levels in vivo by western blot analysis using an HA-tagged rsmA. Our data suggests that the segment of RNA that contains the putative stem-loop structure serves some function in post-transcriptional regulation of RsmA.
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Салуян, М. В. "Android-застосування для читання RSS". Thesis, Чернігів, 2021. http://ir.stu.cn.ua/123456789/23070.
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Салуян, М. В. Android-застосування для читання RSS : випускна кваліфікаційна робота : 123 "Кoмп’ютepнa iнжeнepiя" / М. В. Салуян ; керівник роботи О. А. Пріла ; НУ "Чернігівська політехніка", кафедра iнфopмaцiйниx тa кoмп’ютepниx cиcтeм. – Чернігів, 2021. – 54 с.<br>Метою кваліфікаційної роботи є розробка мобільного додатку для вивчення мови програмування Kotlin і вдосконалення навичків розробки Androidзастосувань. Об’єктом дослідження є технологія RSS, як найкращий спосіб автоматичного отримання оновлень з веб-сайтів. Предметом дослідження є Android-застосування для читання RSS. Для створення застосування використовувалися: інтегроване середовище розробки Android Studio, мова програмування Kotlin та реляційна система керування базами даних SQLite. Результатом роботи є RSS-агрегатор для Android-пристроїв, що виконує функції отримання оновлень з веб-сайтів та їх перегляду. Подальша реалізація застосування можлива шляхом додавання додаткових функцій керування отриманими оновленнями з веб-сайтів та збільшення можливостей налаштування інтерфейсу користувача.<br>The purpose of the qualification work is to learn the programming language Kotlin and improve skills in developing Android applications. The object of research is RSS technology as the best way to automatically receive updates from websites. The subject of the study is an RSS reader Android application. The following resources were used to create the application: Android Studio integrated development environment, Kotlin programming language and SQLite relational database management system. The result of the qualification work is an RSS aggregator for Android devices, which performs the functions of receiving updates from websites and viewing them. Further implementation of the application is possible by adding additional functions to manage updates received from websites and by increasing the ability to customize the user interface.
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Vanejevas, Andrius. "RSS šaltinių kaupimo ir peržiūros sistema." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140716_111629-78245.
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RSS šaltinių kaupimo ir peržiūros sistema sukurta, norint įgyvendinti funkcijas, kurių nėra kitose panašiose sistemose: naujienų siuntimas el. paštu, automatinis naujienų archyvavimas. Kita priežastis, dėl ko sukurta sistema, tai vartotojo sąsajos paprastumas, nereikalaujantis specifinių žinių, kad nekiltų keblumų naudojantis sistemos funkcijomis.
Pirmame darbo skyriuje aprašoma HTML, XML, RSS ir Atom standartai, detalizuoti paveiksliukais ir lentelėmis. Taip pat aprašoma RSS apdorojimo web sprendimai, trumpai aprašomi privalumai, trūkumai ir pateikiama palyginimų lentelė.
Reikalavimų specifikacija parodo, kokias funkcijas turi atlikti sistema. Pateikiama panaudojimo atvejų diagrama, funkciniai ir nefunkciniai reikalavimai. Taip pat aprašomi reikalavimai esamų duomenų perkėlimui ir galimos sistemos kūrimo rizikos.
Trečiame skyriuje, architektūros specifikacija pateikama UML diagramomis: veiklos, sekų, bendradarbiavimo, klasių, esybių ryšių ir komponentų diagramos. Iš pateiktų diagramų galima matyti kaip sistema atrodo iš architektūros pusės ir galima suprasti kaip veikia viena ar kita funkcija.
Testavimo dokumentacijos paskirtis – parodyti dar neatrastas klaidas, kurios gali paveikti visos sistemos funkcionalumą. Sistemos testavimas pateikiamas lentelėse. Buvo atlikti sistemos funkcijų, našumo, suderinamumo ir saugumo testavimai.
Vartotojo vadovas sukurtas, kad parodyti sistemos funkcijų galimybes. Vartotojo vadovas pateikiamas DUK formoje, integruota pačioje sistemos... [toliau žr. visą tekstą]<br>The "Gathering and Viewing System of RSS Sources" was developed in order to implement features that are not presented in other similar systems: send email newsletter to users and automatically archive news items. Another reason for creating a system is the simplicity of the user interface and does not require specific knowledge that avoid problems using the system.
The first chapter describes the HTML, XML, RSS and Atom standards, detailed with pictures and tables. It also provides web solutions for processing RSS feeds, briefly describes the advantages and disadvantages. As a result, web solutions of the RSS feeds represents in comparison table.
Requirements specification indicates what functions have to perform this system. It represents use case diagram, the functional and non-functional requirements. It also, describes the requirements of the existing data transfer and the potential risk of system development.
In the third chapter of architectural specification contains UML diagrams: activity, sequence, collaboration, class, entity and component. From these diagrams you can see how the system looks like from the architecture side, this way you can understand the operation of one or the other function. Also, diagrams are represented in simple and short descriptions, and detailed tables.
The purpose of testing documentation is to show undetected bugs that may affect the overall system’s functionality. The testing of system was detailed in tables. It was carried out of... [to full text]
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Kuhne, Sarah Anna. "RsmA - a global post-transcriptional regulator in Pseudomonas aeruginosa." Thesis, University of Nottingham, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.519398.
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To, Davidnhan D. "Characterization of a novel peptide inhibitor of RsmC function." Kent State University Honors College / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ksuhonors1558543430558686.
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Matveev, Konstantin. "q-deformed Interacting Particle Systems, RSKs and Random Polymers." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493453.
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We introduce and study four $q$-randomized Robinson--Schensted--Knuth (RSK) insertion tableau dynamics. Each of them is a discrete time Markov dynamics on two-dimensional interlacing particle arrays (these arrays are in a natural bijection with semistandard Young tableaux). For $0<q<1$ each dynamics provides a two-dimensional extension of the corresponding one-dimensional exactly solvable random dynamics of interacting particles. We prove that our dynamics act nicely on a certain class of probability measures on arrays, namely, on $q$-Whittaker processes. For $q=0$ these dynamics degenerate to the classical row or column RSK insertion tableau dynamics applied to a random input matrix with independent geometric or Bernoulli entries. We prove that in a scaling limit as $q\nearrow1$, two of our four dynamics on interlacing arrays turn into the geometric RSK dynamics associated with log-Gamma and strict-weak directed random lattice polymers.<br>Mathematics
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Andersson, Ola, and Niclas Larsson. "RSS – The future of internal communication?" Thesis, Växjö University, School of Mathematics and Systems Engineering, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:vxu:diva-377.
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<p>RSS is a format for easy information sharing between computers. A RSS-file, also known as a feed or a channel, consist of a list of items. The items are structured with XML-tags and have to be processed in some way before it can be read. RSS¬readers, web-browsers with RSS support and e-mail-clients with RSS support can be used to display the feed.</p><p>This thesis is focused to investigate if RSS can be used within an enterprise for internal communication. We look at different computer based communication tools that are available today and compare them with RSS. All the different tools has there advantages as well as disadvantages, we have tried to find out if there is room for one more information channel within an enterprise.</p><p>Our study shows that RSS is not suitable as a stand alone solution for internal combination. RSS main strengths lies in mass information that has to be pushed out in one direction.</p>
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Brochard, Jean-Christophe Noël Elisabeth. "Utilisation des fils RSS en bibliothèque." [S.l.] : [s.n.], 2005. http://www.enssib.fr/bibliotheque/documents/dcb/M-2005-RECH-03.pdf.
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Benveniste, Jessica A. "Design and development of a single channel RSNS direction finder." Thesis, Monterey, Calif. : Naval Postgraduate School, 2009. http://edocs.nps.edu/npspubs/scholarly/theses/2009/March/09Mar%5FBenveniste.pdf.
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Thesis (M.S. in Electrical Engineering)--Naval Postgraduate School, March 2009.<br>Thesis Advisor(s): Pace, Phillip E. ; Jenn, David C. "March 2009." Description based on title screen as viewed on April 23, 2009. Author(s) subject terms: Direction Finding, Robust Symmetrical Number System, Single Channel, Angle of Arrival. Includes bibliographical references (p. 77-76). Also available in print.
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Davidsson, Marcus. "Förundersökning och koncept-framtagning av kompletterande produkt till RSS fallskyddssystem : Möjliggörande applicering av RSS fallskyddssystem till tak med fotrännor." Thesis, Karlstads universitet, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-46236.
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Detta är en rapportering av ett examinationsprojekt inom högskoleingenjörsprogrammet i innovationsteknik och design. Uppdragsgivaren och projektbeställaren var Stefan Bäckström, VD för Roofac AB. Syftet med projektet var att göra en förundersökning om möjligheten att applicera RSS (Roof Safety Systems) fallskyddssystem på tak med fotrännor genom en tillbehörsprodukt. Denna förundersökning skulle därmed leda till en kunskapsbank och material som skulle kunna vara till nytta för vidare utvecklingsarbete av tillbehörsprodukten. Syftet var också att göra en bedömning kring potentialen att erhålla en prototyp som är tillräckligt bra att användas som en tillbehörsprodukt. Metoden att uppfylla syftet med projektet och uppnå de uppsatta målen har varit att använda PU-processen som har präglats i utbildningens olika projektarbeten. I planeringen av projektet har verktyg som WBS, PERT-schema, Gant-schema och projektmodell använts för att strukturera projektet. I förstudien har research, användarundersökning och analyser utförts för att samla kunskap och underlag till konceptframtagningsarbetet. Analyserna resulterade i en kravspecifikation som kan användas som ett underlag för utvärdering av genererade koncept. Även en mockup-modell av tak med fotränna har konstruerats för att underlätta testning och utvärdering av fysiska koncept-prototyper. Konceptframtagningsarbetet har utgjorts av idégenerering, konceptgenerering och konceptutvärdering. Idégenereringen baserades på tre idégenereringssessioner, en med ett gäng studenter, en med produktutvecklingsingenjörer på Semcon i Karlstad och en med uppdragsgivaren och kollega på Roofac. Utifrån idéerna genererades sedan CAD-modeller som användes till att generera koncept. Koncepten utvärderades sedan med hjälp av för- och nackdelslista och matris. Resultatet av utvärderingen blev tre slutgiltiga koncept. För att uppnå målet med att leverera en fysisk modell/prototyp att använda som uppvisningsmaterial under utställningen av projektet, avgränsades prototypframtagnings- och testningsarbetet med att gå vidare med den lämpligaste av de tre slutgiltiga koncepten. Från prototypframtagningen och testningen erhölls en fysisk modell av det koncept som valdes att gå vidare med efter konceptframtagningen. Denna modell användes tillsammans med en mockup-modell vid utställningen av projektet. Baserat på resultatet från förstudien, konceptframtagningen, prototypframtagningen samt mockupbygget har en kunskapsbank samt material erhållits att kunna använda i vidare arbeten. Utifrån detta anses därför att syftet med projektet vara uppfyllt.
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Quintero, Yanes Alex Armando. "Novel pleiotropic regulators of gas vesicle biogenesis in Serratia." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288405.
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Serratia sp. ATCC 39006 (S39006) is known for producing carbapenem and prodiginine antibiotics; 1-carbapen-2-em-3-carboxylic acid (car) and prodigiosin. It displays different motility mechanisms, such as swimming and swarming aided by flagellar rotation and biosurfactant production. In addition, S39006 produces gas vesicles to float in aqueous environments and enable colonization of air-liquid interfaces. Gas vesicles are thought to be constructed solely from proteins expressed from a gene cluster composed of two contiguous operons, gvpA1-gvpY and gvrA-gvrC. Prior to this study, three cognate regulators, GvrA, GvrB, and GvrC, encoded by the right hand operon were known to be essential for gas vesicle synthesis. Post-transcriptional regulators such as RsmA-rsmB were also known to be involved in the inverse regulation of gas vesicles and flagella based motility. Furthermore, gas vesicle formation, antibiotic production, and motility in S39006 were affected by cell population densities and de-repressed at high cellular densities through a quorum sensing (QS) system. The aim of this research study was to identify novel regulatory inputs to gas vesicle production. Mutants were generated by random transposon mutagenesis followed by extensive screening, then sequencing and bioinformatic identification of the corresponding mutant genes. After screening, 31 mutants and seven novel regulatory genes impacting on cell buoyancy were identified. Phenotypic and genetic analysis revealed that the mutations were pleiotropic and involved in cell morphology, ion transport and central metabolism. Two new pleiotropic regulators were characterized in detail. Mutations in an ion transporter gene (trkH) and a putative transcriptional regulator gene (floR) showed opposite phenotypic impacts on flotation, flagella-based motility and prodigiosin, whereas production of the carbapenem antibiotic was affected in the transcription regulator mutant. Gene expression assays with reporter fusions, phenotypic assays in single and double mutants, and proteomics suggested that these regulatory genes couple different physiological inputs to QS and RsmA-dependent and RsmA-independent pathways.
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Rangfeldt, Alexander. "Självkänsla och Facebook : En kvantitativ enkätstudie." Thesis, Karlstads universitet, Institutionen för sociala och psykologiska studier (from 2013), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-71961.
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Föreliggande studie är en kvantitativ enkätundersökning som ämnade att undersöka korrelationenmellan global självkänsla och facebookanvändande. Enkäten bestod av Rosenbergs Self-Esteem Scaleoch en egendesignad skala gjord för att mäta facebookanvändande. Enkäten skapades med hjälpSurvey and Report och resultatet analyserades sedan i SPSS där Pearsonsproduktmomentkorrelationskoefficient användes för att räkna ut korrelationen. Deltagarna valdes utgenom ett bekvämlighetsurval, då enkäten delades ut genom Facebook. Resultat visade att det intefinns en signifikant korrelation mellan facebookanvändande och självkänsla.<br>Quantitative survey study about facebook usage and self-esteem. The study used the Rosenberg Self-Esteem Scale.
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Kwai, Kevin Kiah Wen. "An analysis of three-channel RSNS virtual spacing direction finding system." Thesis, Monterey, Calif. : Naval Postgraduate School, 2007. http://bosun.nps.edu/uhtbin/hyperion-image.exe/07Dec%5FKwai.pdf.
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Thesis (M.S. in Combat Systems Science and Technology)--Naval Postgraduate School, December 2007.<br>Thesis Advisor(s): Pace, Phillip E. ; Jenn, David C. ; Walters, Donald L. "December 2007." Description based on title screen as viewed on January 22, 2008. Includes bibliographical references (p. 73-74). Also available in print.
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Brual, Typhaine. "Unraveling virulence regulation in pectinolytic bacteria : Insights from ArcZ and RsmC." Electronic Thesis or Diss., Lyon, INSA, 2023. http://www.theses.fr/2023ISAL0100.
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Les bactéries pectinolytiques du genre Dickeya prospèrent dans diverses niches écologiques, y compris l'eau, le sol et les plantes, s'adaptant à des environnements complexes et en constante évolution, façonnés par diverses interactions biotiques et abiotiques.Dans cette thèse, nous avons étudié les mécanismes qui régulent la virulence du genre Dickeya, en particulier D. dadantii et D. solani.Nos principaux résultats concernent la régulation post-transcriptionnelle exercée par le sRNA ArcZ et la régulation post-traductionnelle modulée par la protéine RsmC. ArcZ est un acteur clé de l'adaptation de D. solani, régulant la motilité en fonction des conditions environnementales et favorisant la virulence lors de l'infection des plantes. En outre, ArcZ joue un rôle essentiel dans la résistance à l’acidité.RsmC, quant à lui, est impliqué dans la régulation de la motilité et joue un rôle complexe dans la virulence. Nos résultats suggèrent de nouvelles interactions pour RsmC et ouvrent des perspectives pour l'étude d'autres fonctions non documentées.En un mot, notre étude révèle comment des régulateurs tels que ArcZ et RsmC orchestrent les réponses bactériennes à un environnement dynamique. Ces résultats mettent en évidence l'adaptabilité des espèces du genre Dickeya et soulignent l'importance du contexte écologique dans l'étude du comportement bactérien<br>Pectinolytic bacteria of the genus Dickeya thrive in diverse ecological niches including water, soil and plants, adapting to complex and ever-changing environments shaped by various biotic and abiotic interactions. In this thesis, we investigated the mechanisms that regulate the virulence of the genus Dickeya, in particular D. dadantii and D. solani. Our main findings concern post-transcriptional regulation exerted by the sRNA ArcZ and post-translational regulation modulated by the protein RsmC. ArcZ is a key player in D. solani adaptation, regulating motility according to environmental conditions and enhancing virulence during plant infection. In addition, ArcZ plays a critical role in acid resistance. RsmC, in turn, is involved in the regulation of motility and has a complex role in virulence. Our results suggest novel interactions for RsmC and open perspectives for studying other undocumented functions. In a word, our study reveals how regulators such as ArcZ and RsmC orchestrate bacterial responses to a dynamic environment. These findings highlight the adaptability of Dickeya species and underscore the importance of ecological context in the study of bacterial behavior
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Roberts, Brian J. "Site-specific RSS signature modeling for WiFi localization." Worcester, Mass. : Worcester Polytechnic Institute, 2009. http://www.wpi.edu/Pubs/ETD/Available/etd-050109-120008/.
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Thesis (M.S.)--Worcester Polytechnic Institute.<br>Keywords: empirical database; WiFi localization; RSS; channel modeling; performance evaluation. Includes bibliographical references (leaves 110-111).
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Li, Qian, and Xintong Zhang. "Indoor and Outdoor Evaluation of Campus RSS Performance." Thesis, Högskolan i Gävle, Avdelningen för elektronik, matematik och naturvetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-9430.
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The focus of this thesis work is to evaluate the RSS (Received Signal Strength) Performance of the University of Gävle (HiG) based on IEEE 802.11 standards both indoor and outdoor. Authors investigated the issues of deploying access points for wireless local area networks in the library-2nd floor, building 99-4th floor and outdoor university campus. By using the program VisiWave Site Survey, Global Position System (GPS) and RSS sensor to analysis the received signal strength, throughput and radio map. The influence of the building material and distance for the signal strength and the throughput are done by investigating indoor environment. The results of investigation suggest that most parts of library-2nd floor and building 99-4th floor possess at least a good RSS performance. However, minority parts of these places have a poor RSS performance, and the new resolution of Access Points’ (AP) deployment for these poor-RSS-performance parts is provided. For the outdoor campus part, the RSS in the area which near the walls of building is satisfactory (Received Signal Strength Indication (RSSI) between -79.8 dBm and -57 dBm), however in the centre of outdoor campus the RSS is poor. Thus, the evaluation of APs deployment in HiG is achieved.
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Grigutis, Valdemaras. "Universalus žiniatinklio srautų aprašymo formatas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2008. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20080619_133430-62328.
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Žiniatinklio srautų technologija neseniai pradėjo augti, tačiau ji sparčiai išplito ir tapo dažnai sutinkama internetiniuose puslapiuose. Dabar yra trys pagrindiniai naudojimi žiniatinklio srauto formatai ATOM 1.0, RSS 2.0 ir RSS 1.0. Šiame darbe yra parašyta nauja žiniatinklio srautų specifikacija, kuri sujungia visus ATOM 1.0, RSS 2.0 ir RSS 1.0 formatus ir pridedami atitinkamai nauji elementai. Kad tai padaryti buvi išanalizuota ATOM 1.0, RSS 2.0 ir RSS 1.0 formatų architektūra. Universalaus žiniatinklio srautų architektūra realizuota sukurtame konvertavimo į universalų žiniatinklio srauto metode, kuris gali būti naudojamas projektuose. Sukurtas metodas verčia ATOM 1.0, RSS 2.0 ir RSS 1.0 formatų dokumentus į šiame darbe aprašytą universalų žiniatinklio srauto formatą ir normalizuoja jį. Rezultatai, gauti po ATOM 1.0, RSS 2.0 ir RSS 1.0 dokumentų konvertavimo į universalų žiniatinklio srauto formatą yra palyginti ir padarytos išvados.<br>Feed technology has only just started growing, but it become very popular and can meet in most web pages. There is at least three major competing feed formats in ATOM 1.0, RSS 2.0 and RSS 1.0 . In this work is written new feed format specification, which joins all ATOM 1.0, RSS 2.0 and RSS 1.0 formats and includes new ones elements. To do this has been analysed ATOM 1.0, RSS 2.0 and RSS 1.0 architekture. Universal feed architekture realized in created universal feed parser method, which can be included in projects. Craeted method replaces ATOM 1.0, RSS 2.0 and RSS 1.0 documents into universal feed document and normalize them. Results got after converting ATOM 1.0, RSS 2.0 and RSS 1.0 feeds into universal feed document is compared and made conclusion.
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Kijek, Jaroslaw Jacek [Verfasser]. "RsfS (YbeB) is an universally conserved ribosome silencing factor / Jaroslaw Jacek Kijek." Berlin : Freie Universität Berlin, 2013. http://d-nb.info/1036872769/34.
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Li, Zeyuan. "Target localization using RSS measurements in wireless sensor networks." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31356.
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The subject of this thesis is the development of localization algorithms for target localization in wireless sensor networks using received signal strength (RSS) measurements or Quantized RSS (QRSS) measurements. In chapter 3 of the thesis, target localization using RSS measurements is investigated. Many existing works on RSS localization assumes that the shadowing components are uncorrelated. However, here, shadowing is assumed to be spatially correlated. It can be shown that localization accuracy can be improved with the consideration of correlation between pairs of RSS measurements. By linearizing the corresponding Maximum Likelihood (ML) objective function, a weighted least squares (WLS) algorithm is formulated to obtain the target location. An iterative technique based on Newtons method is utilized to give a solution. Numerical simulations show that the proposed algorithms achieves better performance than existing algorithms with reasonable complexity. In chapter 4, target localization with an unknown path loss model parameter is investigated. Most published work estimates location and these parameters jointly using iterative methods with a good initialization of path loss exponent (PLE). To avoid finding an initialization, a global optimization algorithm, particle swarm optimization (PSO) is employed to optimize the ML objective function. By combining PSO with a consensus algorithm, the centralized estimation problem is extended to a distributed version so that can be implemented in distributed WSN. Although suboptimal, the distributed approach is very suitable for implementation in real sensor networks, as it is scalable, robust against changing of network topology and requires only local communication. Numerical simulations show that the accuracy of centralized PSO can attain the Cramer Rao Lower Bound (CRLB). Also, as expected, there is some degradation in performance of the distributed PSO with respect to the centralized PSO. In chapter 5, a distributed gradient algorithm for RSS based target localization using only quantized data is proposed. The ML of the Quantized RSS is derived and PSO is used to provide an initial estimate for the gradient algorithm. A practical quantization threshold designer is presented for RSS data. To derive a distributed algorithm using only the quantized signal, the local estimate at each node is also quantized. The RSS measurements and the local estimate at each sensor node are quantized in different ways. By using a quantization elimination scheme, a quantized distributed gradient method is proposed. In the distributed algorithm, the quantization noise in the local estimate is gradually eliminated with each iteration. Simulations show that the performance of the centralized algorithm can reach the CRLB. The proposed distributed algorithm using a small number of bits can achieve the performance of the distributed gradient algorithm using unquantized data.
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Hindmarch, Ian. "Localisation using RSS(D) and site-specific propagation modelling." Thesis, University of Bristol, 2016. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.707748.
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Arslan, Erdal. "Extending the dynamic range of a photonic RSNS ADC using a segmentation approach." Thesis, Monterey, California. Naval Postgraduate School, 2011. http://hdl.handle.net/10945/5492.
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Approved for public release; distribution is unlimited.<br>The trend in high-performance digital receivers is to configure the analog-to-digital converter (ADC) directly after the antenna and low noise amplifier. Direct digitization of wideband antenna signals can be realized using Mach-Zehnder interferometers (MZI) to efficiently fold the RF signal into the optical domain. The robust symmetrical number system (RSNS) is a modular scheme formed using N ? 2 integer sequences and insures that any two successive vectors or paired terms from all N sequences differ by only one integer (integer Gray code property). A photonic ADC preprocessing architecture using the RSNS uses a modulus i m number of comparators at the output of each MZI/photodetector. The total number of comparators with a logic ?1? in each channel represents the integer values within each RSNS sequence. The dynamic range
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Quiles, Rolando. "Challenges of implementing RSS barcodes on hospital unit dose blisters /." Online version of thesis, 2007. http://hdl.handle.net/1850/5468.
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Yeh, ShihYuan. "Development of a digital tracking array with single-channel RSNS and monopulse digital beamforming." Thesis, Monterey, California. Naval Postgraduate School, 2010. http://hdl.handle.net/10945/4956.
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Approved for public release; distribution is unlimited<br>Unmanned aerial vehicles (UAVs) are widely used in military applications, and one of the most common missions is remote sensing. Remote sensing requires UAVs equipped with different kinds of sensors. Information collected by remote sensors must be transmitted back to a ground control station (GCS) to conduct analysis. The majority of UAVs are controlled directly by GCS personnel using radio frequency (RF), line-of-sight (LOS) links. The ground antenna must acquire and then track the UAV signal. A digital phased array allows signal processing functions to be performed in the antenna processor as well as beamforming and tracking. The development of a digital tracking array with single-channel robust symmetrical number system (RSNS) and monopulse digital beamforming (DBF) to track a UAV's transmitted signal is described in this thesis. The RSNS is used as the direction finding (DF) algorithm and can provide high angle resolution with two closely spaced elements. However, as is typical for an array, the angle accuracy is reduced at the two ends of the field-of-view (FOV). The monopulse DBF is used to precisely track the signals. The monopulse tracking technique provides precise angle accuracy within a FOV of approximately ±45. The tracking system is developed in LabView, and the performance of a six-element prototype array is demonstrated by measurement in an anechoic chamber.
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Dalinger, Andrej [Verfasser]. "Der Vertragsbruch des Berufsfußballspielers und die Rechtsfolgen nach Art. 17 FIFA-RSTS / Andrej Dalinger." Baden-Baden : Nomos Verlagsgesellschaft mbH & Co. KG, 2017. http://d-nb.info/1160315353/34.
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Ndima, Nombeko Lungelwa Velile. "The dimensionality of the Rosenberg Self-Esteem Scale (RSES) with South African University Students." Diss., University of Pretoria, 2017. http://hdl.handle.net/2263/61369.
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The Rosenberg Self-Esteem Scale (RSES) has been the subject of widespread debate over the years. Initially conceptualised by Rosenberg as a undimensional measure of global self-esteem, other studies have found evidence that challenges this notion, suggesting that this scale is in fact a multidimensional measure. The aim of this study was to investigate the construct validity of the RSES among South African university students. The RSES was administered to students from two different South African universities located in different regions (N = 304). Principal component analysis (PCA) was used in order to investigate the factor structure of the RSES and correlations were run between the RSES and the General Self-Efficacy Scale (SGSES) to investigate the relationship between self-esteem and self-efficacy. The PCA findings yielded a single factor structure of the RSES in the South African university student sample and a significant positive correlation was observed between self-esteem and self-efficacy. The findings therefore supported the construct validity of the RSES within the South African university context.<br>Mini Dissertation (MA)--University of Pretoria, 2017.<br>Psychology<br>MA<br>Unrestricted
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G, C. Keshav. "Investigation of the Role of Bacterial Ribosomal RNA Methyltransferase Enzyme RsmC in Ribosome Biogenesis." Kent State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=kent1621868567263046.
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Li, Mengchan. "Spatial wireless connectivity prediction for mobile robots." Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-183086.
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Mobile robots, either autonomous or tele-operated have the potential of assisting humans in various situations such as during natural disasters, Urban Search and Rescue (USAR) efforts, and in Explosive Ordinance Disposal (EOD). These robots need steady wireless connectivity with their base station for control and communication. On one hand, the wireless link has to be managed to maintain a stable high quality network connection. On other hand, wireless connection should be continuously monitored to foresee network failure or inadequate link quality situations caused by entering access with low signal strength. This thesis focus on the later where we aim to address the prediction of wireless network connectivity for mobile robots. To indicate wireless connection quality, we use the Radio Signal Strength (RSS) parameter which is readily available by most wireless devices, and it has been frequently used in the literature to indicate wireless connection quality as the RSS have direct relation to the network throughput. Thus the focus of this thesis is to predict the RSS in future robot positions with reference to the current position of the robot. The solution is not straight forward because of the challenging nature of the radio signal propagation which involves complex phenomena such as path loss, shadowing and multipath fading. The RSS prediction method designed in this thesis has two stages. In the first stage, we estimate the location of radio signal source using an RSS gradient-based approach that can work in both single and multiple receivers arrangements. This information will be applied in the next prediction stage. For RSS prediction, we make use of Gaussian Process Regression (GPR) due to non-parametric nature, robustness to noise in the RSS data and changes in the environment. We validate our design with extensive experiments conducted using different types of mobile robots and wireless devices in indoor and outdoor environments, and under line-of-sight (LOS) and non-line-of-sight (NLOS) conditions. We are able to achieve results with source localization error of up to 2 meters for indoor and 5 meters for outdoor environment. In terms of RSS prediction, we obtain the mean absolute prediction error of less than 5 dBm on average, for prediction within 5 meters in indoor environment and 20 meters in outdoor environment. The work is not only promising in terms of prediction time and accuracy but also outperform the state-of-the-art (SOTA) methods including the GPR algorithm, the Kriging interpolation method and the linear regression approaches.
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Hatami, Ahmad. "Application of channel modeling for indoor localization using TOA and RSS." Link to electronic thesis, 2006. http://www.wpi.edu/Pubs/ETD/Available/etd-053106-160141/.
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Nilsson, Martin. "Indoor Positioning Using Opportunistic Multi-Frequency RSS With Foot-Mounted INS." Thesis, Linköpings universitet, Reglerteknik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-111072.
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Reliable and accurate positioning systems are expected to significantly improve the safety for first responders and enhance their operational efficiency. To be effective, a first responder positioning systemmust provide room level accuracy during extended time periods of indoor operation. This thesis presents a system which combines a zero-velocity-update (ZUPT) aided inertial navigation system (INS), using a foot-mounted inertial measurement unit (IMU), with the use of opportunistic multi-frequency received signal strength (RSS) measurements. The system does not rely on maps or pre-collected data from surveys of the radio-frequency (RF environment; instead, it builds its own database of collected rss measurements during the course of the operation. New RSS measurements are continuously compared with the stored values in the database, and when the user returns to a previously visited area this can thus be detected. This enables loop-closures to be detected online, which can be used for error drift correction. The system utilises a distributed particle simultaneous localisation and mapping (DP-SLAM) algorithm which provides a flexible 2-D navigation platform that can be extended with more sensors. The experimental results presented in this thesis indicates that the developed rss slam algorithm can, in many cases, significantly improve the positioning performance of a foot-mounted INS.
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Päivärinta, E. (Elia), and S. (Samuel) Savikoski. "Lisätty todellisuus RSS-syötteiden heijastuksessa ja eri konenäkökirjastojen vertailu sen toteutuksessa." Bachelor's thesis, University of Oulu, 2017. http://urn.fi/URN:NBN:fi:oulu-201705101776.
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Lisätyllä todellisuudella tarkoitetaan virtuaalisen todellisuuden liittämistä ympäröivään todellisuuden, esimerkiksi videon, äänen tai kuvan muodossa. Lisätty todellisuus on lupaava teknologia, jolle löytyy tulevaisuudessa useita mahdollisia tutkimuskohteita ja kaupallisia toteutuksia. Tässä työssä vertaillaan SimpleCV ja OpenCV -konenäkökirjastojen käyttöä ja tehoa Pythonilla käyttämällä niitä RSS-syötteiden heijastukseen lisätyn todellisuuden muodossa. Lisäksi niiden toiminnallisuutta kokeillaan suorituskykytesteillä. Testiohjelmissa vertaillaan tehoa normaalille videokuvalle, Cannyn reunantunnistukselle ja eroosiolle. Näiden testien lisäksi esitellään lisätyn todellisuuden sovellus, joka tehtiin Python-ohjelmointikielellä Raspberry Pi -tietokoneelle. Sovelluksessa tulostetaan RSS-uutissyötteitä kameran kuvaamalle tasolle. Taso löydetään käyttämällä Cannyn reunantunnistusta ja Hough-muunnosta, jotka toteutetaan OpenCV-kirjaston avulla. Käyttäjä voi ohjata ohjelmaa yksinkertaisilla näppäimistön syötteillä<br>Augmented reality means merging virtual reality and surrounding reality together using video, sound or image. Augmented reality is a promising technology with many possible research topics and commercial products. In this bachelor’s thesis SimpleCV and OpenCV computer vision libraries were compared for usability and efficiency. This was done as augmented reality by using them to display RSS-feeds on top of a camera image. The performance and speed were also tested by creating small test programs. In these programs the performance was evaluated for video feed, Canny edge detection and erosion. In addition to these tests an augmented reality program is introduced in this thesis. It was implemented for Raspberry Pi platform using Python programming language. The program shows RSS-feeds on top of a surface found on a camera image. The surface is found using Canny edge detection and Hough transform. OpenCV library was used for the algorithms. The user can control the program with simple keyboard inputs
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Carramate, Fausto Filipe das Neves Dias. "Localização relativa de robôs móveis baseada no RSS de comunicações RF." Master's thesis, Universidade de Aveiro, 2008. http://hdl.handle.net/10773/1966.
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Mestrado em Engenharia Electrónica e Telecomunicações<br>Este estudo sumaria, de forma critica, algumas contribuições científicas
acerca da aplicação de Localização Relativa a Multi-robot Systems.
A comunicação RF entre agentes, quando constituem uma equipa, é
fundamental para que exista consenso nas decisões acerca do estado
da equipa bem como para definir a próxima tarefa a ser executada.
O conceito de Localização Relativa é exigido por estas equipas, na
ausência de referenciais fixos ou âncoras, por exemplo, na manutenção
de conectividade entre agentes. A limitação nas respectivas potências
de transmissão impõe limitações ao seu alcance. Se durante a execução
da tarefa individual de cada agente a conectividade ficar comprometida,
a Localização Relativa apoiará os agentes na sua aproximação.
Apresentamos um método que permite a um agente controlar a sua
locomoção para garantir certos requisitos de Received Signal Strength
(RSS) e conectividade utilizando unicamente informação RSS. Este
efeito consegue-se devido á filtragem das inúmeras interferências que
as comunicações RF sofrem no meio de transmissão.
Por último, sugerimos uma forma, também ela de Localização Relativa,
que é caracterizada por um planeamento de locomoção mais rápido.
Com este estudo indicamos as vantagens e desvantagens da Locali-
zação Relativa quando comparada com a Localização Absoluta. Mos-
tramos que o RSS de comunicações Ad-hoc pode ser utilizado pela
Localização Relativa e ainda no controlo da locomoção para contro-
lar a formação, e certas propriedades da topologia da Mobile Ad-hoc
Network (MANET).
ABSTRACT: This study critically summarises relevant scienti¯c contributions of Re-
lative Localisation applied to Multi-Robot Systems. The system uses
RF communication to reach consensus on the team state as well on
the de¯nition of the next task to be executed. The Relative Localisa-
tion concept is needed by Multi-Robot Systems, when static anchors
are not available, e.g. connectivity management between agents. The
limited transmission power also imposes limitations on transmission
range. When one agent is performing a local task the connectivity can
be put in danger, the Relative Localisation will support the agents on
their approximation.
Our proposal consists on a motion control method to achieve and
maintain certain RSS and connectivity requirements using only the
RSS information. This is possible with an e±cient ¯ltering of the noise
and interference that characterises the RSS information.
We then propose a new method of Relative Localisation which aims to
be quicker than presented early.
With this study we indicate the advantages and disadvantages of Re-
lative and Absolute Localisation. We also show that RSS can be to
generate Relative Localisation for Multi-Robot Systems and can be
used as well to address motion control to achieve formation control,
tracking and topological properties of the MANET.
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Landa, Tobar Luis Ángel. "Modelo de crecimiento del negocio de la firma RSM Auditores Ltda." Tesis, Universidad de Chile, 2014. http://repositorio.uchile.cl/handle/2250/115590.
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Tesis para optar al grado de Magíster en Gestión y Dirección de Empresas<br>El presente documento ha sido elaborado tomando como base el proceso de planificación estratégica que la Firma RSM Chile Auditores Ltda. , está llevando a cabo en su desarrollo de actividades para el crecimiento de la Firma en Chile. La firma internacional de la que es parte, le exige ser la quinta firma de mayor facturación del rubro en Chile, lo que representaría en 2015 $5.300.000.000, con una base de $2.840.000.000.-en 2012 ( incluye $1.100.000.000 de la sociedad relacionada Landa Consultores ,con licencia para representar a RSM ) y un crecimiento requerido de $2.460.000.000 ingresos anuales adicionales, al termino de dicho periodo.-
El objetivo del trabajo es diseñar una estrategia de crecimiento y evaluación de la misma, que pueda ser implementada durante los próximos 3 años. Determinar si es posible alcanzar el crecimiento y si es rentable hacerlo.
Se realiza un diagnóstico de la situación competitiva de la empresa en Chile y un análisis del entorno del modelo de su negocio, identificando las condiciones presentes y los cambios que pueden venir, todo ello en relación con el crecimiento requerido.
Se elabora una propuesta de su modelo de negocio, utilizando CANVAS y luego sobre este se aplica un cuestionario para la evaluación del mismo, (FODA), identificándose brechas que la empresa debe de trabajar para alcanzar el crecimiento requerido, siendo las principales: no se está cobrando por algunos servicios; no se está trabajando la venta de servicios complementarios.
Del análisis de los distintos caminos de crecimiento (Matriz de Ansoff), y considerando las características de la Firma, se eligió crecer aumentando la participación de mercado con marketing directo y la designación de oficinas representantes en regiones.
Como conclusión es posible señalar que la situación de RSM es favorable para alcanzar el crecimiento requerido, definiéndose 2 líneas de acción y con aporte y una rentabilidad cada una de ellas como sigue:
Crecimiento orgánico; Aportaría $1.650.000.000.- representa el 67 % del crecimiento requerido y una rentabilidad del orden del 30 %.
Apertura de nuevas oficinas representantes en regiones; Aportaría $ 800.000.000.-, representa el 33 % del crecimiento y no aportaría a la rentabilidad, salvo para financiar en parte los gastos de la estructura central en materia de marketing, publicidad, capacitación, membrecía. El licenciamiento a empresas representantes en regiones es una opción en la cual están dadas las condiciones para incursionar con éxito toda vez que se cuenta con el conocimiento, la experiencia, la metodología y la reputación necesarias.
Para alcanzar el crecimiento requerido, no está previsto incursionar en un proceso de fusión con otras firmas debido a la complejidad que tiene el unir culturas e intereses. Sin embargo, siempre está abierta esta posibilidad.
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Pashaei, Fakhri Kambiz. "Modélisation des composants d'optique intégrée par la méthode modale (la RSM)." Grenoble INPG, 1998. http://www.theses.fr/1998INPG0147.
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Nous etudions la modelisation des composants d'optique integree par la methode modale. La methode modale est la projection du champ electrique et magnetique sur les modes propres de la structure. La methode modale, nous fourni une meilleure comprehension des effets de rayonnements indispensable a la realisation des circuits d'optique performants. Nous presentons les autres methodes existantes et nous les comparons avec la methode modale. Nous etudions la modelisation d'une discontinuite. Lorsque qu'une onde est incidente sur une discontinuite, une partie de l'onde est reflechie et une autre partie est transmise. Ainsi une partie de la puissance de l'onde incidente est couplee aux modes progressifs et une autre partie aux modes regressifs. Ces modes progressifs et regressifs comprennent non seulement les modes guides mais aussi les modes rayonnes et les modes evanescents. La perte de puissance dans les guides d'onde optique est due aux modes rayonnes. Les modes rayonnes transportent de la puissance active mais les modes evanescents transportent de la puissance reactive. Ces modes sont importants pour la representation fine du champ pres de la discontinuite. Pour la modelisation d'une discontinuite, nous presentons deux methodes. La premiere methode consiste, a projeter les modes progressifs et les modes regressifs sous un systeme d'equation lineaire matricielle. En inversant ce systeme matriciel, on retrouve la puissance reflechie et transmise. Nous presentons ensuite une nouvelle technique pour l'analyse des discontinuites, cette methode nous permet d'eviter les matrices de grosse taille et en meme temps la vitesse et la precision de calcul sont grande. Nous appliquons cette methode originale aux differentes structures et nous comparons les resultats ainsi obtenus avec les resultats obtenus en utilisants les coefficients de reflexion de fresnel. Nous nous interessons ensuite aux reseaux periodiques. Les reseaux periodiques sont important en optique integree pour la realisation des differents composants tels que les filtres et les lasers dfb. Pour la modelisation nous utilisons deux methodes, l'une matricielle et l'autre en faisant appel a des aller-retours multiples. Nous presentons les resultats ainsi obtenus.
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Hmedeh, Zeinab. "Indexation pour la recherche par le contenu textuel de flux RSS." Phd thesis, Conservatoire national des arts et metiers - CNAM, 2013. http://tel.archives-ouvertes.fr/tel-00968604.
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Afin de réduire l'intervalle de temps nécessaire entre la publication de l'information sur le Web et sa consultation par les utilisateurs, les sites Web reposent sur le principe de la Syndication Web. Les fournisseurs d'information diffusent les nouvelles informations à travers des flux RSS auxquels les utilisateurs intéressés peuvent s'abonner. L'objectif de la thèse est de proposer un système de notification passant à l'échelle du Web, prenant en considération le grand nombre d'utilisateurs et le débit élevé d'items. Nous proposons un index basé sur les mots-clés des requêtes utilisateurs permettant de retrouver ceux-ci dans les items des flux. Trois structures d'indexation de souscriptions sont présentées. Un modèle analytique pour estimer le temps de traitement et l'espace mémoire de chaque structure est détaillé. Nous menons une étude expérimentale approfondie de l'impact de plusieurs paramètres sur ces structures. Pour les souscriptions jamais notifiées, nous adaptons les index étudiés pour prendre en considération leur satisfaction partielle. Afin de réduire le nombre d'items reçus par l'utilisateur, nous intégrons une deuxième phase de filtrage par nouveauté et diversité considérant l'ensemble d'items déjà reçus par l'utilisateur.
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Hmedeh, Zeinab. "Indexation pour la recherche par le contenu textuel de flux RSS." Electronic Thesis or Diss., Paris, CNAM, 2013. http://www.theses.fr/2013CNAM0894.
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Afin de réduire l’intervalle de temps nécessaire entre la publication de l’information sur le Web et sa consultation par les utilisateurs, les sites Web reposent sur le principe de la Syndication Web. Les fournisseurs d’information diffusent les nouvelles informations à travers des flux RSS auxquels les utilisateurs intéressés peuvent s’abonner. L’objectif de la thèse est de proposer un système de notification passant à l’échelle du Web, prenant en considération le grand nombre d’utilisateurs et le débit élevé d’items. Nous proposons un index basé sur les mots-clés des requêtes utilisateurs permettant de retrouver ceux-ci dans les items des flux. Trois structures d’indexation de souscriptions sont présentées. Un modèle analytique pour estimer le temps de traitement et l’espace mémoire de chaque structure est détaillé. Nous menons une étude expérimentale approfondie de l’impact de plusieurs paramètres sur ces structures. Pour les souscriptions jamais notifiées, nous adaptons les index étudiés pour prendre en considération leur satisfaction partielle. Afin de réduire le nombre d’items reçus par l’utilisateur, nous intégrons une deuxième phase de filtrage par nouveauté et diversité considérant l’ensemble d’items déjà reçus par l’utilisateur<br>Based on a Publish/Subscribe paradigm, Web Syndication formats such as RSS have emerged as a popular means for timely delivery of frequently updated Web content. According to these formats, information publishers provide brief summaries of the content they deliver on the Web, while information consumers subscribe to a number of RSS feeds and get informed about newly published items. The goal of this thesis is to propose a notification system which scales on the Web. To deal with this issue, we should take into account the large number of users on the Web and the high publication rate of items. We propose a keyword-based index for user subscriptions to match it on the fly with incoming items. We study three indexing techniques for user subscriptions. We present analytical models to estimate memory requirements and matching time. We also conduct a thorough experimental evaluation to exhibit the impact of critical workload parameters on these structures. For subscriptions which are never notified, we adapt the indexes to support a partial matching between subscriptions and items. We integrate a diversity and novelty filtering step in our system in order to decrease the number of notified items for short subscriptions. This filtering is based on the set of items already received by the user
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Johansson, Robert. "Investigation of the Turbulent Flow and Heat Transfer around a Heated Cube Cooled by Multiple Impinging jets in a Cross-Flow." Thesis, Högskolan i Gävle, Avdelningen för bygg- energi- och miljöteknik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-21851.
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The fast development in electronics has resulted in faster and faster computers. Furthermore, the electronic components trend to get smaller and smaller by the year. With more processing power combined with smaller components the heat generation rapidly increases. The scope of this study is to examine a spot cooling technique consisting with different geometry of multiple impinging jets in combination with a cross-flow by the use of CFD. The case is limited to a heated wall mounted cube cooled by a impinging jet as well as an multiple impinging jets in a low velocity cross-flow. This study can be divided into two parts a verification study and a detailed study. The verification study consist of comparison between RSM model and measured values for both the turbulent flow and the surface temperature. The single impinging mesh consists of 934 k elements while the plus 1439 k and cross consists of 2809 k elements. All the meshes are created in ANSYS fluent and this paper contains a detailed guide to create them. The verification study proved that RSM can predict the complicated flow with good agreement with the single impinging jet. The heat transfer coefficient differ substantially between the cases. The PIV compared to the UDF for the inlet velocity profiles had a 21\% increase in heat transfer coefficient in the top layer of the cube. In all the simulations the cross had at least an increase of 18\% on average \(h\). While there was no real verification study for the multiple impinging jets I would still argue that cross is better than the plus sign geometry in terms of heat transfer.
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Gaskell, Alisa A. "Genetic and biochemical characterisation of Streptomyces coelicolor sigma factor, δм, and its regulator, RsmA." Thesis, University of East Anglia, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435022.
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Almeida, Valéria Gentil. "O esverdeamento da economia e os tributos verdes : um duro caminho rumo à sustentabilidade da gestão dos resíduos sólidos urbanos (RSU)." reponame:Repositório Institucional da UnB, 2016. http://repositorio.unb.br/handle/10482/21003.
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Tese (doutorado)—Universidade de Brasília, Centro de Desenvolvimento Sustentável, 2013.<br>Submitted by Fernanda Percia França (fernandafranca@bce.unb.br) on 2016-06-08T16:17:35Z
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2013_ValériaGentilAlmeida.pdf: 4692627 bytes, checksum: 5923acdcf5925c5f6201a7b43161531d (MD5)<br>O esverdeamento da economia é o novo termo de ordem associado ao desenvolvimento sustentável, particularmente após a Conferência Rio + 20. A economia verde, ou green economics, utiliza um conjunto de metodologias que permitem, de uma forma mais concreta e prática, promover mudanças no âmbito regulatório, nos processos produtivos e atitudes dos consumidores. Ela se apresenta agnóstica em relação ao método, pois suas metodologias fundamentam-se, de modo objetivo, na transição para uma economia verde. Nos moldes norteadores do esverdeamento da economia, o objetivo geral da presente Tese diz respeito a um dos setores chave desta, ao de reciclagem e reaproveitamento de resíduos sólidos urbanos (RSU), e consiste, notadamente, em sistematizar e interpretar as perspectivas de aplicação da tributação verde nesse setor, com base no levantamento do estado-da-arte do conhecimento relativo ao tema no mundo. A pesquisa está embasada nos procedimentos metodológicos de investigação exploratória e pesquisa qualitativa. Conclui-se que as metas e estratégias da economia verde são claras: uma economia de baixo carbono, eficiência dos recursos, investimentos verdes, inovação tecnológica e maior reciclagem, empregos verdes, erradicação da pobreza e inclusão social. Ênfase especial é dada a uma estrutura de política verde adequada, pondo-se em foco as questões de base como as dificuldades ainda enfrentadas na gestão dos resíduos sólidos no Brasil. O estudo de caso foi realizado no Bairro de Perus, localizado na zona noroeste da capital São Paulo/SP, cuja população representa um recorte característico da realidade socioeconômica do Brasil. Os resultados desse estudo demonstram a necessidade e a urgência das medidas políticas voltadas para a implantação e a utilização dos instrumentos baseados no mercado, como as taxas verdes, no intuito de promover o esverdeamento da economia brasileira. Se, por um lado, a pesquisa de campo revelou uma disposição da população em adotar práticas mais sustentáveis, a partir de um reconhecimento de que a má gestão traz impactos ambientais negativos, com reflexos inclusive para a qualidade de vida nas cidades, por outro, verificou-se que a população precisa assumir uma postura mais pró-ativa, e cabe ao Estado oferecer tais instrumentos econômicos capazes de alterar o quadro atual de baixa eficiência na gestão verde das cidades. ________________________________________________________________________________________________ ABSTRACT<br>The greening of the economy is the new term of order associated with sustainable development, particularly after the Conference Rio + 20. Green economics uses a set of methodologies that allow for a more concrete and practical way to make changes within the scope of regulations, production processes and consumer attitudes. It seems to be agnostic in relation to method, because its underlying methodologies, objectively point to the transition to a green economy. Modeled after the guiding principles of the "greening the economy”, the general aim of this thesis concerns one of its key sectors , the recycling and reuse of urban solid waste , and notedly consists in the systematization and interpretation of perspectives of implementing green taxation in this sector, based on the survey of the state-of-the-art knowledge on the subject in the world. The researchis based on the methodological procedures of exploratory research and qualitative research. We conclude that the goals and strategies of the green economy are clear: an economy of low carbon, resource efficiency, green investments, technological innovations and increased recycling, green jobs, poverty erradication and social inclusion. Special emphasis is given to a structure suitable to green policies, putting into focus basic questions such as the difficulties still faced in the management of solid waste in Brazil. The case study was conducted in the Perus neighborhood, located in the north west of the capital São Paulo/SP, whose population represents a characteristic sample of socio-economic reality in Brazil. The results of this study demonstrate the necessity and urgency of policy measures aimed at the implementation and use of market-based instruments such as green taxes, in order to promote the greening of the Brazilian economy. If, on the one hand, the field research revealed a willing ness of the population to adopt more sustainable practices resulting from are cognition that poor management brings negative environmental consequences with effects that impact the quality of life in cities, on the other hand, it was found that the population needs to take a more pro-active attitude, and it is up to the state to provide the economic instruments that can change the current situation of low efficiency in the green management of cities. ________________________________________________________________________________________________ RESUMÉ<br>L'écologisation de l'économie est un nouveau mot d’ordre associé au développement soutenable, spécialement après la Conférence de Rio + 20. L'économie verte, ou green economics, signifie un ensemble de méthodologies qui permettent de promouvoir, d’une façon plus concrète et pratique, une évolution dans le domaine réglementaire, dans les processus de production et les attitudes des consommateurs. Elle se présente agnostique, car ses méthodologies justifient, objectivement, la transition vers une économie verte. En conformité avec les principes de l'économie verte, l'objectif général de cette Thèse porte sur l'un des secteurs clés de celle-ci, le recyclage et la réutilisation des déchets solides urbains (DSU), et consiste, particulièrement, à systématiser et à interpréter les perspectives d'application de la fiscalité verte dans ce secteur, en prenant comme base une enquête des connaissances relatives à ce sujet qui sont accumulées dans le monde. La recherche est basée sur les procédures méthodologiques de la recherche exploratoire et de la recherche qualitative. On conclut que les objectifs et les stratégies de l'économie verte sont clairs: une economie de basse teneur de carbone, l'efficacité énergétique, les investissements verts, l'innovation technologique et l’accroissement du recyclage, les emplois verts, l'éradication de la pauvreté et l’inclusion sociale. Une attention particulière est accordée à la structure de la politique verte adéquate, en mettant l'accent sur les questions fondamentales, y comprises les difficultés encore présentes dans la gestion des déchets solides au Brésil. L'étude de cas a été effectuée dans le quartier de Perus, situé dans la partie nord-ouest de la capitale São Paulo / SP, dont la population représente une tranche caractéristique de la réalité sociale et économique du Brésil. Les résultats de l'étude démontrent la nécessité et l’urgence des mesures politiques destinées à l’implantation et l’utilisation des instruments dérivés du marché, telles que les taxes vertes, dans le but de promouvoir l’écologisation de l’économie brésilienne. Si, d'une part, la recherche sur le terrain a révélé une volonté de la population à adopter des pratiques plus durables, de la reconnaissance que la mauvaise gestion apporte impacts négatifs sur l'environnement, avec des effets, y compris la qualité de vie dans les villes, d'autre part, il a été constaté que la population doit adopter une approche plus pro-active, et il appartient à l'Etat de fournir les instruments économiques capables de changer la situation actuelle de la faible efficacité dans la gestion verte des villes.
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Gomes, Keila Adiene Guimarães. "Caracterização dos resíduos sólidos de serviços de saúde do Hospital Escola Hélvio Auto em Maceió AL, visando a elaboração e implantação do plano de gerenciamento de resíduos." Universidade Federal de Alagoas, 2011. http://repositorio.ufal.br/handle/riufal/423.
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The environmental impacts related to inadequate management of solid wastes have been widely discussed by society. Healthcare wastes (HCW) is part of this equation thus deserves special attention. The qualitative and quantitative characterization of this waste is fundamental to the development and implementation of a Waste Management Plan of Health Services (WMPHS), identifying the percentage of special and common wastes. This study aimed to characterize the waste generated in the University Hospital Hélvio Auto and propose measures to prevent, minimize, reuse and recycle in order to avoid environmental and human contamination. The method adopte for waste characterization was based on the guidelines of the Pan American Health Organization (PAHO, 1997) and Souza (2005). The period of characterization was from May/2009 to May/2010, for better assessment of seasonal variation. The results indicated that most of the waste generated in the study area is the common type (85.3%) and recycling potential. The infectious waste, sharps and chemicals accounted for 5.5, 7.0 and 2.2% respectively of total waste generated in the hospital. Such a waste, if mishandled, can compromise all mass of waste, preventing the reuse and recycling. The average production of RSS was 0,5 kg / patient.day. Data from qualitative and quantitative characterization of the waste subsides the elaboration of WMPHS in the study area, required by law, the RDC N° 306/2004 ANVISA (National Agency for Sanitary Vigilance), Ministry of Health. As a waste measurement of maximization of reusable wastes, is proposed a proper segregation, at the very source of the material, in order to enable the reuse and recycling. For the effective management of waste, at the hospital, or at any other institution providing health services, it is necessary to stimulate a permanent awareness programs, from the button to top of its hierarchical levels.<br>Os impactos ambientais relacionados ao manejo inadequado dos resíduos sólidos são amplamente discutidos pela sociedade. Os resíduos de serviços de saúde (RSS) se inserem nesta problemática e merecem atenção especial. A caracterização quali-quantitativa desses resíduos é fundamental para a elaboração e implantação de um Plano de Gerenciamento de Resíduos de Serviços de Saúde (PGRSS), com a identificação da porcentagem de resíduos especiais e comuns. Este estudo teve como objetivo caracterizar os resíduos gerados no Hospital Escola Hélvio Auto (HEHA) e propor medidas para prevenção, minimização, reutilização e reciclagem, visando evitar a contaminação ambiental e humana. O método adotado para caracterização dos resíduos foi baseado nas orientações da Organização Pan-Americana de Saúde (OPAS, 1997) e Souza (2005). O período de caracterização compreendeu os meses de maio/2009 a maio/2010, para melhor avaliação da variação sazonal. Os resultados indicaram que a maior parte dos resíduos gerados na área de estudo é do tipo comum (85,3%) e com potencial de reciclagem. Os resíduos infectantes, perfurocortantes e químicos responderam por 5,5, 7,0 e 2,2%, respectivamente, do total de resíduos gerados no HEHA. Estes, se mal manejados, podem comprometer toda a massa de resíduos, inviabilizando a reutilização e reciclagem. A produção média de RSS foi de 0,5 Kg/paciente.dia Os dados de caracterização quali-quantitativa dos resíduos subsidiaram a elaboração do PGRSS na área de estudo, exigido pela legislação vigente, a RDC Nº 306/2004 ANVISA (Agência Nacional de Vigilância Sanitária) do Ministério da Saúde. Como medida de maximização de resíduos reaproveitáveis, sugere-se a adequada segregação de material na fonte de geração para viabilizar a reutilização e reciclagem. Para a gestão eficaz de resíduos, no HEHA, ou em qualquer outra instituição prestadora de serviço de saúde, deve-se estimular ações permanentes de sensibilização, em todos os seus níveis hierárquicos.
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Wald, Jean François. "Lois de paroi adaptatives pour un modèle de fermeture du second ordre dans un contexte industriel." Thesis, Pau, 2016. http://www.theses.fr/2016PAUU3014/document.
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Les calculs de CFD industriels pour les écoulements turbulents commencent par une phase complexe de réalisation de maillage (calculs de fond de cuve, de plénum supérieur ou d’assemblages combustibles par exemple dans le domaine nucléaire). Les premières contraintes prises en compte sont le plus souvent géométriques (complexité, détail, intuition ou retour d'expérience concernant les endroits « importants » où le maillage doit être raffiné). On doit cependant respecter des contraintes inhérentes aux modèles de turbulence RANS (Reynolds Averaged Navier Stokes) utilisés notamment la taille de la première cellule de calcul à la paroi. Si on utilise un modèle dit « Haut-Reynolds » (k- ε standard, SSG, …), on ne devrait trouver que des cellules de paroi ayant un centre à une distance adimensionnelle au moins égale à 20 pour pouvoir d’une part justifier l'utilisation de la loi « universelle » logarithmique pour la vitesse et d’autre part, ce qui souvent occulté, respecter le fait que ces modèles ne sont pas conçus pour des distances plus basses. En revanche, si on utilise un modèle dit « Bas-Reynolds » (BL-v²/k, EB-RSM, …), on devrait partout avoir des cellules de paroi ayant un centre à une distance adimensionnelle de la paroi très faible. Si ces modèles sont utilisés avec une partie des cellules en paroi ayant une distance adimensionnelle nettement supérieure, les résultats peuvent être catastrophiques (le calcul peut ou bien diverger ou bien donner des résultats avec une physique totalement fausse). Cette thèse propose le développement d'un nouveau modèle de turbulence avec lois de paroi adaptatives qui donne des résultats satisfaisants quelque soit le type de maillage utilisé, en particulier quand ce dernier contient à la fois des cellules dont le centre est à une distance « Bas-Reynolds » et « Haut-Reynolds ». Étant donné les écoulements complexes des configurations industrielles, ce nouveau modèle s'appuie sur l'utilisation d'un modèle du second ordre connu pour son bon comportement : le modèle EB-RSM. Ce modèle permet de reproduire l'anisotropie de la turbulence et comble certaines lacunes des modèles du premier ordre. Ce modèle est disponible dans Code_Saturne, code open source développé par EDF et au sein duquel les développements ont été réalisés<br>CFD computations of turbulent flows always begin with a complex meshing process (upper plenum, fuel assembly in the nuclear industry for example). Geometrical constraints are the first ones to be satisfied (level of details, important zones to refine regarding “user experiences”). One has however to satisfy constraints that are inherent to the RANS model (Reynolds Averaged Navier Stokes) used for the computation. For example, if a « High-Reynolds » (k-ε standard, SSG, …) model is used one should only have wall cells with a dimensionless distance to the wall greater or equal to 20 to justify the use of the universal “law of the wall”. On the other hand, if a « Low-Reynolds » (BL-v²/k, EB-RSM, …) model is used, one should only find wall cells with a dimensionless distance to the wall below 1. If those models are used in an inappropriate way the results could be dramatic (computations can either diverge or give unphysical results). This thesis proposes the development of a new turbulence model with adaptive wall treatments that gives satisfactory results on all types of meshes. In particular, the model will be able to cope with meshes containing both « High-Reynolds » and « Low-Reynolds » wall cells. Given the complex flows encountered in the nuclear industry this thesis will use a model known for its good behavior: the EB-RSM model. This model is able to reproduce the anisotropy of the turbulence and give more satisfactory results than eddy viscosity models in different configurations. This model is available in Code_Saturne, an open source code developed at EDF. Al the developments are made in this code
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Lau, Shuk-kwan, and 劉淑君. "An immunological approach to rolling horizon order pickup scheduling for AS/RSs." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B4440668X.
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O'Shea, Martin. "A series of case studies to enhance the social utility of RSS." Thesis, Birkbeck (University of London), 2016. http://bbktheses.da.ulcc.ac.uk/276/.
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RSS (really simple syndication, rich site summary or RDF site summary) is a dialect of XML that provides a method of syndicating on-line content, where postings consist of frequently updated news items, blog entries and multimedia. RSS feeds, produced by organisations or individuals, are often aggregated, and delivered to users for consumption via readers. The semi-structured format of RSS also allows the delivery/exchange of machine-readable content between different platforms and systems. Articles on web pages frequently include icons that represent social media services which facilitate social data. Amongst these, RSS feeds deliver data which is typically presented in the journalistic style of headline, story and snapshot(s). Consequently, applications and academic research have employed RSS on this basis. Therefore, within the context of social media, the question arises: can the social function, i.e. utility, of RSS be enhanced by producing from it data which is actionable and effective? This thesis is based upon the hypothesis that the fluctuations in the keyword frequencies present in RSS can be mined to produce actionable and effective data, to enhance the technology's social utility. To this end, we present a series of laboratory-based case studies which demonstrate two novel and logically consistent RSS-mining paradigms. Our first paradigm allows users to define mining rules to mine data from feeds. The second paradigm employs a semi-automated classification of feeds and correlates this with sentiment. We visualise the outputs produced by the case studies for these paradigms, where they can benefit users in real-world scenarios, varying from statistics and trend analysis to mining financial and sporting data. The contributions of this thesis to web engineering and text mining are the demonstration of the proof of concept of our paradigms, through the integration of an array of open-source, third-party products into a coherent and innovative, alpha-version prototype software implemented in a Java JSP/servlet-based web application architecture.