Relevant bibliographies by topics / RT-PCR - Arrays

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'RT-PCR - Arrays'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "RT-PCR - Arrays"

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Hessner, Martin J., Bixia Xiang, Shuang Jia, et al. "Three-color cDNA microarrays with prehybridization quality control yield gene expression data comparable to that of commercial platforms." Physiological Genomics 25, no. 1 (2006): 166–78. http://dx.doi.org/10.1152/physiolgenomics.00243.2005.

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Despite their lower cost and high content flexibility, a limitation of in-house-prepared arrays has been their susceptibility to quality control (QC) issues and lack of QC standards across laboratories. Therefore, we developed a novel three-color array system that allows prehybridization QC as well as the Matarray software to facilitate acquisition of accurate gene expression data. In this study, we compared performance of our rat cDNA array to the Affymetrix RG-U34A and Agilent G4130A arrays using 2,824 UniGenes represented on all three arrays. Before data filtering, poor interplatform agreem
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Bergqvist, Cecilia, Petra Holmström, Gunnel Lindegren, Nina Lagerqvist, Mikael Leijon, and Kerstin I. Falk. "Multiplex Nucleic Acid Suspension Bead Arrays for Detection and Subtyping of Filoviruses." Journal of Clinical Microbiology 53, no. 4 (2015): 1368–70. http://dx.doi.org/10.1128/jcm.02787-14.

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Here we describe multiplex suspension bead array systems that allow fast and reliable detection of reverse transcriptase (RT) PCR amplified filovirus genomes and also enable subtyping of Ebola virus species and Marburg virus strains. These systems have an analytical sensitivity equivalent to that of RT-PCR.
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Osman, Fatima, Christian Leutenegger, Deborah Golino, and Adib Rowhani. "Comparison of low-density arrays, RT-PCR and real-time TaqMan® RT-PCR in detection of grapevine viruses." Journal of Virological Methods 149, no. 2 (2008): 292–99. http://dx.doi.org/10.1016/j.jviromet.2008.01.012.

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Arikawa, Emi, Min You, Jie Wang, Jing-yi Lo, Sean Yu, and Jingping Yang. "Real-time PCR array for simultaneous evaluation of multiple cytokine mRNA expression (B204)." Journal of Immunology 178, no. 1_Supplement (2007): LB42—LB43. http://dx.doi.org/10.4049/jimmunol.178.supp.b204.

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Abstract Cytokine quantification is an important element in studies of inflammation and immune responses. Quantitative RT-PCR, a rapid and sensitive assay, is the preferred method to quantify cytokine mRNA levels because they are often expressed at low levels. The RT2Profiler™ PCR Array combines the reliable performance of SYBR® Green based RT-qPCR with multi-gene profiling capabilities to simultaneously analyze the expression of a panel of genes from the same pathway. Using PCR Arrays, we have monitored the mRNA levels of 84 different cytokines in human peripheral blood mononuclear cells (PBM
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Ida, Hisashi, Sharon A. Boylan, Andrea L. Weigel, and Leonard M. Hjelmeland. "Age-related changes in the transcriptional profile of mouse RPE/choroid." Physiological Genomics 15, no. 3 (2003): 258–62. http://dx.doi.org/10.1152/physiolgenomics.00126.2003.

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To evaluate the age-related changes in gene expression occurring in the complex of retinal pigmented epithelium, Bruch’s membrane, and choroid (RPE/choroid), we examined the gene expression profiles of young adult (2 mo) and old (24 mo) male C57BL/6 mice. cDNA probe sets from individual animals were synthesized using total RNA isolated from the RPE/choroid of each animal. Probes were amplified using the Clontech SMART system, radioactively labeled, and hybridized to two different Clontech Atlas mouse cDNA arrays. From each age group, three independent triplicates were hybridized to the arrays.
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Yildirim, Fatih, M. Enes Aslan, Hüseyin daştan, et al. "Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs." Pakistan Journal of Medical and Health Sciences 15, no. 10 (2021): 2928–32. http://dx.doi.org/10.53350/pjmhs2115102928.

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Introduction and Purpose: Recent studies revealed that different behavioral and physiological processes are closely related to different receptor levels in humans. On the other hand, there weren’t any studies that investigated neurotransmitter activity and entrepreneurship relationships. Little is known about how genes expressed in the blood are associated with entrepreneurship. In this study, we measured the expression of 84 genes encoding neurotransmitter receptors between the entrepreneurs and non-entrepreneurs (n=25) using RT-PCR arrays to monitor differentially expressed genes for explori
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Yildirim, Fatih, M. Enes Aslan, Hüseyin Daştan, et al. "Comparative Expression Analysis of Genes Encoding Neurotransmitter Receptors Between Entrepreneurs and Non-Entrepreneurs." Pakistan Journal of Medical and Health Sciences 15, no. 9 (2021): 3058–62. http://dx.doi.org/10.53350/pjmhs211593058.

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Introduction and Purpose: Recent studies revealed that different behavioral and physiological processes are closely related to different receptor levels in humans. On the other hand, there weren’t any studies that investigated neurotransmitter activity and entrepreneurship relationships. Little is known about how genes expressed in the blood are associated with entrepreneurship. In this study, we measured the expression of 84 genes encoding neurotransmitter receptors between the entrepreneurs and non-entrepreneurs (n=25) using RT-PCR arrays to monitor differentially expressed genes for explori
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Brokx, Stephen J., Michael Ellison, Troy Locke, Drell Bottorff, Laura Frost, and Joel H. Weiner. "Genome-Wide Analysis of Lipoprotein Expression in Escherichia coli MG1655." Journal of Bacteriology 186, no. 10 (2004): 3254–58. http://dx.doi.org/10.1128/jb.186.10.3254-3258.2004.

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ABSTRACT To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions—aerobic, anaerobic, and anaerobic with nitrate—were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR). Many genes showed significant changes in expression level. The RT-PCR results were in very good agreement with the microarray data. The results of this study represent the first insights into th
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Li, Chun-Hong, Yong-Qing Zhu, Yu-Ling Meng, et al. "Isolation of genes preferentially expressed in cotton fibers by cDNA filter arrays and RT-PCR." Plant Science 163, no. 6 (2002): 1113–20. http://dx.doi.org/10.1016/s0168-9452(02)00320-5.

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Schuchardt, Eleanor L., Shelley D. Miyamoto, Timothy Crombleholme, et al. "Amniotic Fluid microRNA in Severe Twin-Twin Transfusion Syndrome Cardiomyopathy—Identification of Differences and Predicting Demise." Journal of Cardiovascular Development and Disease 9, no. 2 (2022): 37. http://dx.doi.org/10.3390/jcdd9020037.

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Twin-twin transfusion syndrome (TTTS) is a rare but serious cause of fetal cardiomyopathy with poorly understood pathophysiology and challenging prognostication. This study sought a nonbiased, comprehensive assessment of amniotic fluid (AF) microRNAs from TTTS pregnancies and associations of these miRNAs with clinical characteristics. For the discovery cohort, AF from ten fetuses with severe TTTS cardiomyopathy were selected and compared to ten normal singleton AF. Array panels assessing 384 microRNAs were performed on the discovery cohort and controls. Using a stringent q < 0.0025, arrays
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Dissertations / Theses on the topic "RT-PCR - Arrays"

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Deo, Ameya. "Normalization of microRNA expression levels in Quantitative RT-PCR arrays." Thesis, University of Skövde, School of Life Sciences, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-4133.

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<p><strong>Background:</strong> Real-time quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) is recently used for characterization and expression analysis of miRNAs. The data from such experiments need effective analysis methods to produce reliable and high-quality data. For the miRNA prostate cancer qRT-PCR data used in this study, standard housekeeping normalization method fails due to non-stability of endogenous controls used. Therefore, identifying appropriate normalization method(s) for data analysis based on other data driven principles is an important aspect of this
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Brandt, Stephan Peter. "Zelltyp-spezifische Mikroanalyse von Arabidopsis thaliana-Blättern." Phd thesis, Universität Potsdam, 2001. http://opus.kobv.de/ubp/volltexte/2005/40/.

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Im ersten Teil der Arbeit wurden Strategien zur Analyse von Transkripten erarbeitet. Die ersten Versuche zielten darauf ab, in mit Glaskapillaren genommenen Einzelzellproben verschiedener Gewebeschichten RT-PCR durchzuführen, um spezifische Transkripte nachweisen zu können. Dies gelang für eine Reihe von Genen aus verschiedenen Pflanzenspezies. Dabei konnten sowohl Transkripte stark wie auch schwach exprimierter Gene nachgewiesen werden. <br /> Für die Erstellung von Gewebe-spezifischen Expressionsprofilen war es notwendig, die in vereinigten Zellproben enthaltene mRNA zunächst zu amplifiziere
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Book chapters on the topic "RT-PCR - Arrays"

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Barcroft, Lisa C., D. Holstead Jones-Taggart, and Andrew J. Watson. "Antisense Disruption of Gene Expression in the Preimplantation Embryo." In A Laboratory Guide To The Mammalian Embryo. Oxford University PressNew York, NY, 2004. http://dx.doi.org/10.1093/oso/9780195142266.003.0017.

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Abstract Development of techniques such as reverse transcription-polymerase chain reaction (RT- PCR), differential display RT-PCR, and, more recently, the use of gene-array chips has allowed a number of developmentally regulated gene products within the preimplantation embryo to be identified and characterized. Many of these gene products are involved in cell adhesion (1–10), inter- and intracellular signaling (11–19), cell cycle regulation (20–23), autocrine regulation (24–36), embryo metabolism (37–46), and ion transport (47–56). Despite extensive investigation of the timing of mRNA and prot
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Scotton, Chris, and Frances Burke. "Northern analysis, RT-PCR, RNase protection, cDNA array systems, and in situ hybridization to detect cytokine messenger RNA." In Cytokine Molecular Biology. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199638581.003.0003.

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Abstract There are various methods used to detect cytokine mRNA in tissues and cell lines and the commonly used ones are described in this chapter. The varying degrees of sensitivity and suitability for use are discussed. Northern analysis of RNA allows the quantitation of specific messenger RNA (mRNA) sequences and the determination of their size. The RNA sample is separated by electrophoresis through a denaturing agarose gel followed by capillary transfer to a membrane to which the RNA is subsequently covalently linked. The mRNA of interest is located by probing the membrane with a labelled
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Conference papers on the topic "RT-PCR - Arrays"

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Parisi, Cleo, Athina Markou, Areti Strati, and Evi Lianidou. "Abstract 1462: Development of a multiplexed RT-PCR-coupled liquid bead array assay for the molecular characterization of CTCs." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-1462.

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Ahmed, Ola, Hisham Imam, and Abdel-Rahman N. Zekri. "Abstract 2914: Gold magneto nanoparticles induced apoptosis in liver cancer cells through changes of Ca channel pump detected by LIBS Technique and RT-PCR array." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-2914.

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Murphy, Patricia, Melissa Adams, Brennan Martin, Elizabeth Horlick, and Douglas Malinowski. "Abstract 818: Correlation of mRNA and protein expression of CA125 and HE4 in ovarian cancer tissues using real-time RT-PCR and reverse phase protein array technologies." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-818.

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Pereira, JD, KBG Borges, MTA Vieira, KF Rodrigues, KTOS Jorge, and FM Soriani. "EXPRESSÃO DE MIRNAS RELACIONADOS COM A RESPOSTA INFLAMATÓRIA EM PACIENTES COM DIABETES MELLITUS TIPO 2." In Resumos do 54º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2022. http://dx.doi.org/10.5327/1516-3180.140s1.5763.

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Objetivo: O diabetes mellitus (DM) é uma doença que atinge cerca de 15,733 milhões de indivíduos no mundo. O principal tipo é o diabetes mellitus tipo 2 (DM2), caracterizado pela resistência à insulina, processo inflamatório subcrônico; representa cerca de 90% dos casos da doença. O DM2 é um importante fator de risco para distúrbios cardiovasculares e um dos problemas é o seu diagnóstico tardio. Assim, novos marcadores para o diagnóstico precoce da doença são necessários. Os miRNAs são pequenos RNAs não codificantes endógenos, envolvidos na regulação da expressão gênica; são promissores marcad
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Ahmed, Sumaya, and Nasser Rizk. "The Expression of Bile Acid Receptor TGR5 in Adipose Tissue in Diet-Induced Obese Mice." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0212.

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Bile acids are significant physiological factors for digestion, solubilization, absorption, toxic metabolites and xenobiotics. In addition, bile acids are responsible of signal transduction as well as metabolic regulation that activate several receptors such as farnesoid X receptor (FXR) and the membrane G-protein receptor 5 (TGR5). Activation of TGR5 by bile acids is associated with prevention of obesity as well as ameliorating the resistance to insulin via increasing energy expenditure. The objective of this research is to investigate TGR5 gene expression level in different fat depots includ
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Al-Qeraiwi, Maha, Manar Al-Rashid, Nasser Rizk, Abdelrahman El Gamal, and Amena Fadl. "Hepatic Gene Expression Profile of Lipid Metabolism of Obese Mice after treatment with Anti-obesity Drug." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0214.

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Obesity is a global disorder with multifactorial causes. The liver plays a vital role in fat metabolism. Disorder of hepatic fat metabolism is associated with obesity and causes fatty liver. High fat diet intake (HFD) to mice causes the development of dietinduced obesity (DIO). The study aimed to detect the effects of anti-obesity drugs (sulforaphane; SFN and leptin) on hepatic gene expression of fat metabolism in mice that were fed HFD during an early time of DIO. Twenty wild types (WT) CD1 male mice aged ten weeks were fed a high fat diet. The mice were treated with vehicle; Veh (control gro
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Rodrigues, Antonio Rony da S. P., and Edinalda Maria Cavalcante. "ESTUDO GENÉTICO DO CÂNCER DE TIREOIDE – UMA REVISÃO." In I SIMPÓSIO MARANHENSE DE GENÉTICA E GENÔMICA EM SAÚDE. Doity - Plataforma de Eventos, 2022. http://dx.doi.org/10.55664/simaggens2022.005.

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INTRODUÇÃO: O câncer de tireoide é um dos agravos mais comuns ao sistema endócrino, com o maior aumento anual de incidência em diferentes países, principalmente devido à melhoria das tecnologias de diagnóstico, sendo mais proeminente em regiões com acesso aos cuidados de saúde amplamente disponíveis. Alguns eventos moleculares são descritos tanto na carciogênese da tireoide quanto na evolução do tumor glandular. Dados do Atlas do câncer no Genoma Humano dividiu os carcinomas papilíferos de tireoide nas categorias BRAF e RAS, com base nos resultados do exoma do sequenciamento de DNA, RNA e perf
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Reports on the topic "RT-PCR - Arrays"

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Porat, Ron, Gregory T. McCollum, Amnon Lers, and Charles L. Guy. Identification and characterization of genes involved in the acquisition of chilling tolerance in citrus fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7587727.bard.

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Citrus, like many other tropical and subtropical fruit are sensitive to chilling temperatures. However, application of a pre-storage temperature conditioning (CD) treatment at 16°C for 7 d or of a hot water brushing (HWB) treatment at 60°C for 20 sec remarkably enhances chilling tolerance and reduces the development of chilling injuries (CI) upon storage at 5°C. In the current research, we proposed to identify and characterize grapefruit genes that are induced by CD, and may contribute to the acquisition of fruit chilling tolerance, by two different molecular approaches: cDNA array analysis an
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Hindson, B., B. Baker, L. Bentley Tammero, et al. Diagnostic evaluation of a multiplexed RT-PCR microsphere array assay for the detection of foot-and-mouth and look-alike disease viruses. Office of Scientific and Technical Information (OSTI), 2007. http://dx.doi.org/10.2172/922093.

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