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Dissertations / Theses on the topic 'RT-PCR (Reverse Transcription Polymerase Chain Reaction)'

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Published: 2 June 2025

Last updated: 31 July 2025

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1

Saunders, Daniel Curtis. "Microfluidic system with open loop control for rapid infrared reverse transcription quantitative PCR (RT-qPCR)." Thesis, Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/44865.

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Microfluidic techniques have allowed for fast, sensitive, and low cost applications of the Polymerase Chain Reaction (PCR) through the use of small reaction volumes, rapid amplification speeds, and the on-chip integration of upstream and downstream sample handling processes including purification and electrophoretic separation functionality. While such systems are capable of measuring the expression levels of thousands of genes simultaneously, or in hundreds of cells, or with sample-in and answer-out capability, none of these systems are easily scalable in the time domain. Because of this, t

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2

Lekas, Poli. "Analysis of human CYP2E1 mRNA in a HepG2 cell line by reverse transcription-polymerase chain reaction (RT-PCR)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0014/MQ40842.pdf.

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3

Zhang, Tieling 1968. "Gene expression analysis of the proteinase inhibitor gene PiA of potato (Solanum tuberosum) by reverse transcription polymerase chain reaction (RT-PCR)." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79212.

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In this study the gene expression pattern of a novel proteinase inhibitor II (PI-II) gene, PiA, in potato (Solanum tuberosum ), was analyzed using RT-PCR. The PiA gene was found to have enhanced expression in expanding tubers of a greenhouse-grown diploid clone (DC), different stages of 'Russet Burbank' tubers and expanding microtubers of in vitro-grown 'Russet Burbank' and 'Bintje'. PiA mRNA was more abundant in the tuber vascular ring and perimedullary zone areas than in other tuber tissues of DC. The PiA gene was also expressed in flower buds/flowers, shoot apices, leaf blades, stems

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4

Bontoux, Nathalie. "Analyse du transcriptome d'une cellule unique à l'aide d'une puce microfluidique." Paris 6, 2006. http://www.theses.fr/2006PA066600.

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5

Kwan, See-wai Grace. "Detection of human coronavirus infections by reverse transcription PCR in children hospitalized with respiratory disease in Hong Kong /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31494274.

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6

Wires, Shannon Meredith. "Rapid detection of Norwalk-like viruses by reverse transcription-polymerase chain reaction (RT-PCR) and southern blot hybridisation (SBH) in outbreaks of acute gastroenteritis in eastern Ontario, Canada." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ59412.pdf.

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7

Chehtane, Mounir. "REAL TIME REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION FOR DIRECT DETECTION OF VIABLE MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS IN CROHN S DISEASE PATIENTS and ASSOCIATION OF MAP INFECTION WITH DOWNREGUALTION IN INTERFERON-GAMMA RECEPTOR (INFG." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4281.

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Association of Mycobacterium avium subspecies paratuberculosis (MAP) with Crohn's disease (CD) and not with ulcerative colitis (UC), two forms of inflammatory bowel disease (IBD), has been vigorously debated in recent years. This theory has been strengthened by recent culture of MAP from breast milk, intestinal tissue and Blood from patients with active Crohn's disease. Culture of MAP from clinical samples remained challenging due to the fastidious nature of MAP including its lack of cell wall in infected patients. The advent of real time PCR has proven to be significant in infectious disease

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8

Lam, Siu-yan. "Multiplex reverse transcription-PCR for detection and identification of human parainfluenza viruses 1,2,3 and 4 infection in hospitalized children with respiratory disease in Hong Kong /." View the Table of Contents & Abstract, 2007. http://sunzi.lib.hku.hk/hkuto/record/B3848058X.

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9

Chan, Kit-man, and 陳潔雯. "Detection of human enteroviruses by reverse transcription-PCR in hospitalized children with respiratory disease in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44669963.

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10

Lassauniere, Maria Magdalena. "Development of quantitative multiplex real-time RT-PCR assays for detection of 13 conventional and newly discovered viruses associated with lower respiratory tract infections in children in South Africa." Diss., University of Pretoria, 2010. http://hdl.handle.net/2263/28457.

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Acute lower respiratory tract infection (ALRI) is a major cause of paediatric morbidity and mortality, annually accounting for approximately 2 million childhood deaths worldwide of which up to 90% resides in the developing world. In 12-39% of ALRI cases no aetiological agent is identified, despite comprehensive investigations, thus suggesting that additional unknown agents may be involved. Since 2001 a number of new viruses have been identified that may account for some of these cases including human metapneumovirus (hMPV), human bocavirus (hBoV), and two new coronaviruses (hCoV) NL63 and HKU1

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Lam, Siu-yan, and 林小欣. "Multiplex reverse transcription-PCR for detection and identification of human parainfluenza viruses 1,2,3 and 4 infection in hospitalizedchildren with respiratory disease in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B45011357.

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12

Mildenberger, Michael [Verfasser], Tobias [Gutachter] Reuther, and Heike [Gutachter] Walles. "Untersuchung von im Tissue-Engineering-Verfahren hergestellten Oral-Mukosa-Äquivalenten mittels RT-qPCR (reverse transcription quantitative real-time polymerase chain reaction) / Michael Mildenberger ; Gutachter: Tobias Reuther, Heike Walles." Würzburg : Universität Würzburg, 2017. http://d-nb.info/1147682070/34.

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13

Odendaal, Lieza. "Sensitivity and specificity of rRT-PCR, histopathology, and immunohistochemistry for the detection of rift valley fever virus in naturally-infected cattle and sheep." Diss., University of Pretoria, 2014. http://hdl.handle.net/2263/40707.

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Rift Valley fever (RVF) is a mosquito-borne zoonotic disease caused by a virus of the family Bunyaviridae, genus Phlebovirus. It is responsible for extensive outbreaks of disease in livestock in Africa with significant mortality and economic impact. Virus neutralization is considered the gold standard for confirming Rift Valley fever virus (RVFV) infection but the procedure is time consuming and expensive. Real-time reverse transcription-polymerase chain reaction (rRT-PCR), histopathology, and immunohistochemistry (IHC) are the diagnostic methods most often used in South Africa to confir

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14

Iglezias, Silvia D\'Andretta. "Endomiocardiofibrose: patologia e correlação clínica em material de ressecção cirúrgica." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/5/5144/tde-02062008-113135/.

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INTRODUÇÃO: A endomiocardiofibrose (EMF) é uma miocardiopatia de padrão restritivo de etiologia desconhecida, prevalente em regiões tropicais. Caracteriza-se por espessamento fibroso do endocárdio e miocárdio subjacente, comprometendo ponta e via de entrada de um ou de ambos os ventrículos. Sua etiopatogenia é pouco conhecida e muitos autores a associam à doença infecciosa cardíaca ou sistêmica, à eosinofilia prévia e/ou à carência nutricional. O prognóstico em geral é grave e a ressecção cirúrgica da lesão é indicada aos pacientes com insuficiência cardíaca refratária a tratamento clínico, em

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15

劉永棠 and Wing-tong Ricky Lau. "Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970667.

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16

Lau, Wing-tong Ricky. "Multiplex RT-PCR for typing and subtyping influenza and respiratory syncytial viruses." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25151599.

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17

Häuptli, Daniel. "Use of reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of vaccine contamination by avian leukosis virus /." [S.l.] : [s.n.], 1996. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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18

Noach, Liesl Christine. "The molecular characterization of South African isolates of Grapevine Rupestris Stem Pitting-associated virus (GRSPaV)." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5252.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2010.<br>Includes bibliography.<br>ENGLISH ABSTRACT: The first aim of this study was to reliably and rapidly detect Grapevine rupestris stem pittingassociated virus (GRSPaV) in grapevine. This was achieved by screening 94 grapevines using crude plant extracts in both quantitative and conventional reverse transcription polymerase chain reaction (RT-PCR). The second aim was to establish a technique capable of differentiating GRSPaV sequence variants. The application of this technique is for the largescale screening of diseased vines to as

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19

Weir, Kenneth. "A refinement to the semi-quantitative RT-PCR method and defense related, stress resistance and insulin pathway gene expression during Sarcophaga crassipalpis diapause." Diss., Online access via UMI:, 2008.

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20

Carter, Kristina Kim. "A Comparison of Centrifugal Forces to Reduce the Inhibitory Effects of Food Matrixes on Reverse Transcriptase Polymerase Chain Reaction for the Detection of Food Borne Viruses." Scholar Commons, 2004. https://scholarcommons.usf.edu/etd/984.

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The CDC estimated that foodborne infections resulted in approximately 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths per year in the United States (Mead, 1999). There are over 200 known diseases caused by viruses, bacteria, parasites, toxins, metals, or prions that can be transmitted through food. Of these illnesses caused by foodborne disease, the CDC estimates that 38.6 million cases are from identifiable pathogens and 30.9 million of these cases are caused by viruses. Hence, approximately 80% of foodborne illnesses of known etiology result from viral transmission (Mead, 19

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21

Lopes, Marissol Cardoso. "Comparação entre as técnicas de RT-PCR e inoculação intracerebral em camundongos para detecção do vírus da raiva em amostras mantidas por longos períodos em diferentes estados de conservação /." Araçatuba : [s.n.], 2008. http://hdl.handle.net/11449/94733.

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Orientador: Luzia Helena Queiroz da Silva<br>Banca: Avelino Albas<br>Banca: Silvana Regina Favaretto Lazarini<br>Resumo: As análises antigênica e genética são ferramentas importantes para o estudo da epidemiologia da raiva em uma região. A recuperação e reisolamento viral a partir de amostras conservadas por longos períodos em temperatura de congelamento é essencial para estudos retrospectivos. Porém, o tempo de conservação, associado a repetidos ciclos de congelamento e descongelamento, promove uma perda significativa na viabilidade do vírus, condição esta que pode ser contornada com a utiliz

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Lopes, Marissol Cardoso [UNESP]. "Comparação entre as técnicas de RT-PCR e inoculação intracerebral em camundongos para detecção do vírus da raiva em amostras mantidas por longos períodos em diferentes estados de conservação." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/94733.

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Made available in DSpace on 2014-06-11T19:27:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-12-12Bitstream added on 2014-06-13T20:35:37Z : No. of bitstreams: 1 lopes_mc_me_araca.pdf: 187442 bytes, checksum: e5c382aecfe4bb38f157a656bfad5f2a (MD5)<br>As análises antigênica e genética são ferramentas importantes para o estudo da epidemiologia da raiva em uma região. A recuperação e reisolamento viral a partir de amostras conservadas por longos períodos em temperatura de congelamento é essencial para estudos retrospectivos. Porém, o tempo de conservação, associado a repetidos ciclos d

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23

de, Albuquerque Andreia, Ilja Kubisch, Georg Breier, et al. "Multimarker Gene Analysis of Circulating Tumor Cells in Pancreatic Cancer Patients: A Feasibility Study." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-133530.

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Objective: The aim of this study was to develop an immunomagnetic/real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and assess its clinical value for the molecular detection of circulating tumor cells (CTCs) in peripheral blood of pancreatic cancer patients. Methods: The presence of CTCs was evaluated in 34 pancreatic cancer patients before systemic therapy and in 40 healthy controls, through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 [targeting mucin 1 and epithelial cell adhesion molecule (EpCAM), respectively], followed by real-time RT-PCR analysis

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de, Albuquerque Andreia, Ilja Kubisch, Georg Breier, et al. "Multimarker Gene Analysis of Circulating Tumor Cells in Pancreatic Cancer Patients: A Feasibility Study." Karger, 2012. https://tud.qucosa.de/id/qucosa%3A27513.

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Objective: The aim of this study was to develop an immunomagnetic/real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and assess its clinical value for the molecular detection of circulating tumor cells (CTCs) in peripheral blood of pancreatic cancer patients. Methods: The presence of CTCs was evaluated in 34 pancreatic cancer patients before systemic therapy and in 40 healthy controls, through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 [targeting mucin 1 and epithelial cell adhesion molecule (EpCAM), respectively], followed by real-time RT-PCR analysis

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25

Santos, Tatiana Nayara Libório dos. "Expressão de variantes transcricionais do gene Homeobox TGIF1 em carcinomas epidermóides de boca." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-09042009-115621/.

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Genes da família homeobox têm sido alvo de intensas pesquisas científicas relacionadas ao câncer. Recentemente, mostramos que o gene homeobox TGIF1 está expresso no carcinoma epidermóide de boca na sua forma genérica. Porém, não foi feita uma discriminação entre quais variantes transcricionais, ou subtipos, do TGIF1 estariam expressas. Neste estudo, propusemo-nos a verificar diferenças na freqüência de expressão das variantes transcricionais do TGIF1 em carcinomas epidermóides de boca (CEB) em relação a tecidos morfologicamente não tumorais (TN), avaliar possíveis associações entre essas varia

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26

Smith, Matthew Adams. "Evaluation and implementation of a molecular-based protocol for the identification of enteroviruses at the Florida Department of Health - Tampa Laboratory." [Tampa, Fla.] : University of South Florida, 2003. http://purl.fcla.edu/fcla/etd/SFE0000164.

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27

Jacobsen, Jan. "Vascular endothelial growth factor in renal cell carcinoma." Doctoral thesis, Umeå : Kirurgisk och perioperativ vetenskap, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-713.

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28

Shiang, Wang-Yu, and 王裕翔. "A development of RT-PCR machine with Reverse Transcription and Polymerase Chain Reaction in a Capillary Loop Tube." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/6b6bjk.

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碩士<br>國立臺灣大學<br>機械工程學研究所<br>107<br>Capillary Loop Convective Polymerase Chain Reaction (CLCPCR) is a new application of convective PCR. It can complete the temperature cycle of PCR with a single heater. By heating the bottom of the loop container, the fluid would form a Rayleigh–Bénard convection cell and generate a circulating flow continuously. This new method can economize more time,energy,cost than traditional PCR machine. Our lab has researched the topic of PCR for many years.But the final purpose is to develop Reverse Transcription PCR,because genetic material of virus is composed of

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29

Wu, Po-Chen, and 吳柏辰. "Development of a 4-in-1 multiplex reverse-transcription polymerase chain reaction (RT-PCR) detection method and survey of four banana viruses in Taiwan." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/r67m2j.

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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>107<br>Banana is one of the most important fruits in the world and ranks the third of export crop in Taiwan. In addition to Panama disease caused by Fusarium oxysporum f.sp. cubense (Foc), the banana plants also face with the threat from different viruses such as cucumber mosaic virus (CMV), banana bunchy top virus (BBTV), banana streak virus (BSV) and banana bract mosaic virus (BBrMV). These four viruses cause various symptoms including stunting, foliar chlorosis or mottling, poor fruit production and decline. CMV and BBTV are the most widespread and serious in

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30

Mildenberger, Michael. "Untersuchung von im Tissue-Engineering-Verfahren hergestellten Oral-Mukosa-Äquivalenten mittels RT-qPCR (reverse transcription quantitative real-time polymerase chain reaction)." Doctoral thesis, 2017. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-155286.

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Im Rahmen dieser Arbeit wurden Fibroblasten und Keratinozyten, welche in vitro auf unterschiedlichen Scaffolds sowohl gemeinsam als auch in Monokulturen gezüchtet wurden, mittels Real-time PCR auf ihre Genausschüttung untersucht, um festzustellen wie sich die Unterlage auf die Genausschüttung auswirkt. Hierzu wurden die Proben sowohl auf die Genexpressionsmarker für die Basallamina Kollagen IV, Laminin 1 und 5 als auch auf die Genexpressionsmarker für die frühe Differenzierung Keratin K13 und K14 untersucht. Als Referenzgen wurde β-Actin ausgewählt, da dieses Gen in den Vorversuchen mit zwei w

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31

尚君璽. "Establishment of a dengue virus surveillance system in mosquitoes by reverse-transcriptase polymerase chain reaction(RT-PCR)." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/17086677866100135495.

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碩士<br>國立臺灣大學<br>流行病學研究所<br>85<br>Dengue fever has become the most important arboviral infectious disease in the 20 century because of the increasing of incidence rates and expanding of the epidemic areas. In 1981, a DEN-2 outbreak exploded in Liu-Chiu Hsiang(琉球鄉). Since then, dengue cases were reported each year in Taiwan. There are four serotypes of dengue viruses and the high indices of mosquito vectors in Taiwan, so that potential risk of DHF/DSS outbreak in the main island for the coming years is increasing. Currently, the surveillance system of dengue in Taiwan depends mainly on physi

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32

"Identification of differentially expressed genes in fibroblasts from human hypertrophic scars by using differential display RT-PCR technique." 1998. http://library.cuhk.edu.hk/record=b5889768.

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by Cheng Chi Wa.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 1998.<br>Includes bibliographical references (leaves 110-120).<br>Abstract also in Chinese.<br>Title --- p.i<br>Abstract --- p.ii<br>Acknowledgement --- p.iv<br>Abbreviations --- p.v<br>Abbreviation Table for Amino Acids --- p.vi<br>Table of Contents --- p.vii<br>List of Figures --- p.xii<br>List of Tables --- p.xv<br>Chapter Chapter 1 --- Introduction --- p.1<br>Chapter Chapter 2 --- Literature review --- p.2<br>Chapter Part I --- Hypertrophic Scar<br>Chapter 2.1 --- Definition of hypertrophic scar --- p.2<br>C

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Liou, Miaw-Jene, and 劉妙真. "Reverse transcriptase polymerase chain reaction (RT-PCR) of thyroid relevant genes in thyroid tumors: a potential implication of preoperative molecular diagnosis of thyroid tumors." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/60627238574925143156.

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碩士<br>長庚大學<br>臨床醫學研究所<br>90<br>Thyroid cancer is the eighth leading cancer among female Taiwanese. Although many scientists have done a lot of researches to investigate this disease, the tumorigenesis of thyroid tumors remains unclear. Fine-needle aspiration (FNA) had been widely established as the key diagnostic tool for preoperative selection of nodules at risk of malignancy. However, preoperative diagnosis of thyroid cancer, especially for those diagnosed as follicular neoplasm (up to 30% in all thyroid tumors) is challenging most physicians. To verify a reliable tumor marker, which can hel

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