Journal articles on the topic 'Rubber tree. eng'

Crude rubber seed oil (CRSO) is a promising but currently underutilized biodiesel feedstock alternative, extracted by pressing the seeds of the rubber tree (Hevea brasiliensis). Rubber trees are cultivated across more than 11.4 million hectares worldwide, mainly in Southeast Asia. Despite their suitability as a biodiesel feedstock source, rubber seeds are currently treated as waste in the monocultural plantation system. To date, no assessments have been performed to examine the potential impact of rubber seed-based biodiesel production on GHG emissions. This study analyses the global warming potential of rubber seed methyl ester (RSME) production in Southeast Asia. The functional unit used is 1 MJ of biodiesel. A sensitivity analysis assesses the influence of key parameters (e.g., rubber seed yield) on the GHG mitigation potential. A scenario analysis evaluates the effect of using RSME by-products for energy generation. In comparison to fossil diesel, RSME has a carbon mitigation potential of 67 g CO2.eq. MJ−1, based on allocation by mass. On the condition of compliance with international sustainability standards that call for deforestation-free value chains, the generation of RSME biodiesel on rubber tree plantations in Southeast Asia would have a total mitigation potential of around 2.8 million tonnes of CO2 eq. per year.

ABSTRACT The objective of this study was to evaluate organic substrates in the production of canafistula (Peltophorum dubium) (Spreng.) Taub, cutieira (Joannesiaprinceps Vell.), jatoba (Hymenaea courbaril L.) and rubber tree (Hevea brasiliensis M. Arg.) seedlings, native trees with potential use in forest restoration programs. The design was completely randomized with 10 substrate formulations with 4 repetitions of 3 plants for the four species. The evaluated substrates consisted of soil, bovine manure (BM), poultry manure (PM), chemical fertilizer (CF) and sand, in different proportions. The experiment was concluded at the end of 180 days for canafistula, cutieira and rubber and 210 days for jatoba. At the end of these periods, the root (RDM), shoot (SDM) and total (TDM) the dry matters of the seedlings were determined. Quantification of AMF spores and normalization between samples through SPORES/RDM correction were also performed. The Scott-Knott test at 5% probability was applied. Regarding biomass production, only canafistula had significant difference among the tested substrates. In relation to sporulation, the highest values were observed in cutieira and rubber tree in substrate containing PM. The substrates composed of 40 or 50% soil + 20% sand + 30% or 40 PM for canafistula; 50% soil + 20% sand + 30% PM for cutieira; and for jatoba and rubber tree 60% soil + 20% sand + 20% PM, enabled the best results in terms of biomass production in seedlings and AMF sporulation.

Trunk phloem necrosis (TPN) is a physiological disease of rubber tree (Hevea brasiliensis Müll. Arg.) discovered in the 1980s. It has been distinguished from rubber tree tapping panel dryness (TPD) by its macroscopic symptoms and presumed origin. But little attention has been paid to its microscopic features, and there is now some evidence that both syndromes could be linked to an impaired cyanide metabolism. In order to characterise TPN and compare it with TPD microscopically, the inner phloem of tapping panels was investigated by light and transmission electron microscopy in healthy trees and TPN-affected trees. TPN-affected phloem presented numerous and varied structural and ultrastructural features. There were signs of cellular deterioration in a great number of specialised cells, i.e. laticifers and sieve tubes, and not very specialised cells, i.e. parenchyma cells and companion cells. There were also signs of cellular dedifferentiation in other parenchymatous cells, e.g. in tylosoids and hyperplasic cells. These cells were derived from parenchyma cells that ensheath laticifers in which the latex coagulated. Numerous structural features of TPN are common to TPD, notably tylosoids associated with in situ coagulated latex, which are also known to be early structural markers of TPD and cyanide-induced. It is therefore concluded that TPN is identical to or a variant of TPD, and is a degenerative disease of rubber tree trunk phloem resembling plant stress response, programmed cell death and plant tumourigenesis in some aspects.

We first reported Alternaria heveae (E.G. Simmons ) to be the pathogen that caused black leaf spot of rubber tree (Hevea brasiliensis Muell. Arg) in Heikou county in July 2014 (1). Black leaf spots that resembled the symptoms caused by A. heveae were observed on the leaves of rubber trees of the whole propagule collection nursery in Jingping County (22°68′ N and 103°05′ E) of Yunnan Province. Black foliar spots (0.1 to 2 mm in diameter) surrounded by a yellow halo with lesions slightly sunken on the leaf surface were observed. To confirm whether the disease was caused by the same pathogen, 5-mm2 sections were removed from the leading edge of the lesion and were surface-sterilized in 75% ethanol, air-dried, plated on potato carrot agar (PCA), and incubated at 28°C in the dark. Colonies of the fungus on PCA had round margins and little aerial mycelia with gray-black coloration after 6 days of growth on PCA (2). Medium brown conidia were found to be in short chains of two to eight spores, ovoid, obclavate, and obpyriform, with or without a short conical or cylindrical-shaped apical beak. Conidia ranged from 22.5 to 67.5 μm long (mean 39.9 μm) × 10 to 15 μm wide (mean 12.5 μm; 100 colodia were measured), with three to six transverse septa and zero to three longitudinal or oblique septa. Morphological characteristics matched the descriptions of A. alternata [(Fries) Keissler] (4).The ITS1-5.8S-ITS2 region of one single-spore isolate, Ah02JP1, was amplified with primers ITS1 and ITS4. The PCR product was sequenced directly and deposited in GenBank (Accession No. KM111289). A BLAST search of the GenBank database revealed 100% similarity with A. alternata isolates KJ829535.1, KJ677246.1, and KF813070.1. Therefore, the pathogen was identified as A. alternata on the basis of its morphological characteristics and ITS sequence. Pathogenicity of a representative isolate, Ah02JP1 was confirmed using a field rubber tree inoculation method. Three rubber plants (the clone of rubber tree Yunyan77-4) were grown to the copper-colored leaf stage. Leaves were spray-inoculated (104 conidia per milliliter spore suspension) until drops were equally distributed using a manual pressure sprayer. Three rubber plants sprayed with sterile distilled water were used as controls. After inoculation, the plants were covered with plastic bags to maintain high relative humidity. The plastic bags were removed 2 days post-inoculation (dpi), and the plants were monitored daily for symptom development. Five days post-inoculation, spots similar to the original ones seen on the field trees developed on all inoculated leaves, while control leaves remained symptomless. A. alternata was re-isolated from spray-inoculated leaves, confirming Koch's postulates. A. alternata has been reported as the causal agent of leaf blight of rubber tree in India, which initially appeared as minute spots on leaves and enlarged with the growth of the leaves (3). However, in the present study, the symptoms (black leaf spots) remained small over time after inoculation. To our knowledge, this is the first report of A. alternata on rubber tree in China. Correct identification of pathogens is essential for disease management strategies. This report will establish a foundation for the further study of Alternaria alternata to address the disease effectively. References: (1) Z. Y. Cai et al. Plant Dis. 98:1011, 2014. (2) E. Mirkova. J. Phytopathol. 151:323, 2003. (3) C. B. Roy et al. J. Plantation Crops 34:499, 2006. (4) T. Y. Zhang. Page 32 in: Flora Fungorum Sinicorum, Vol. 16: Alternaria. Science Press, Beijing, 2003.

The rubber agroforestry experiments in Jambi started with the theory of change that productive clonal rubber could be economically used in low-labour intensity rubber agroforests, allowing selective retention of forest species or planted fruit trees in interrows. At the end of what was expected to be a 25-year production cycle we revisited the farmers (or their next generation), recorded what had happened to the plot and registered farmer plans for a way forward. Qualitatively, the results showed a wide range of directions of actual change. The envisaged plots, with full-grown tapped rubber in a secondary forest setting did occur – but as exception rather than rule. Some plots had early on been converted to oil palm when white root rot disease killed many of the rubber trees. Others were in a gradual transition to oil palm, already interplanted, or depended on natural regeneration of rubber within the plot for the trees currently being tapped. Some plots had been completely destroyed as the land was sold to a local coal-mine developer. Overall tapping frequency was low, as farmgate rubber prices have in recent years been low and farmers had other options (including participating in small-scale gold mining). Farmer experience with the various clones tested led to mixed opinions on which (if any) of the clones introduced were superior to what farmers used in the past (and what still regenerates in the landscape). GT1, a robust clone, was seen as hardly more productive as local germplasm, the PB260 and BPM1 clone were productive, but especially PB260 clone sensitive to white root rot disease. The quality of rubber wood was a concern for some farmers. The most successful intervention, from farmers’ as well as environmental perspective, has probably been the interplanting of meranti (Shorea leprosula) or tembesu (Fagraea fragrans) trees in young rubber stands, with good prospects for generating substantial income.

The phenomenon of rubber consumption in Malaysia has been increased parallel with the world demand. Hence, it is necessary for the cultivators to gain some knowledge for differentiating various rubber tree clone breed which may give them high latex yield after 5 years or more of planting. This paper discusses the identification of several rubber tree clones breed based on the best percentage of the reflectance wavelength obtained from the respected latex. In this work, there were three selected rubber tree clones which are RRIM 2025, PB 355 and PB 350 suggested by Rubber Research Institute of Malaysia (RRIM) will undergo a preliminary analysis on the significant information of spectrum properties acquired from the latex. A hundred of latex samples have been collected from each clone and extracted the optical measurements of the primary color spectrum (Red, Green and Blue) using spectrometer. The next step is to analyze the acquired information based on its reflectance wavelength with respect to clones and at the end it will be evaluated statistically. As a result, there were two out of three clones could be discriminated between each other with refer to the green wavelength (573nm).

Rubber tree (Hevea brasiliensis) is an important crop in tropical regions of China. In October 2013, a new stem rot disease was found on cv. Yunyan77-4 at a rubber tree plantation in Hekou, Yunnan Province. There were about 100 plants, and diseased rubber trees accounted for 30% or less. Initially, brown-punctuate secretion appeared on the stem, which was 5 to 6 cm above the ground. Eventually, the secretion became black and no latex produced from the rubber tree bark. After removing the secretion, the diseased bark was brown putrescence, but the circumambient bark was normal. Upon peeling the surface bark, the inner bark and xylem had brown rot and was musty. The junction between health and disease was undulate. On the two most serious plants, parts of leaves on the crown were yellow, and the root near the diseased stem was dry and puce. The pathogen was isolated and designated HbFO01; the pathogenicity was established by following Koch's postulates. The pathogen was cultivated on a potato dextrose agar (PDA) plate at 28°C for 4 days. Ten plants of rubber tree cv. Yunyan77-4 were selected from a disease-free plantation in Haikou, Hainan Province, and the stem diameter was about 7 cm. The bark of five plants was peeled, and one mycelium disk with a diameter of 1 cm was inserted into the cut and covered again with the bark. The other five plants were treated with agar disks as controls. The inoculation site was kept moist for 2 days, and then the mycelium and agar disk were removed. On eighth day, symptoms similar to the original stem lesions were observed on stems of inoculated plants, while only scars formed on stems of control plants. The pathogen was re-isolated from the lesions of inoculated plants. On PDA plates, the pathogen colony was circular and white with tidy edges and rich aerial hyphae. Microscopic examination showed microconidia and chlamydospores were produced abundantly on PDA medium. The falciform macroconidia were only produced on lesions and were slightly curved, with a curved apical cell and foot shaped to pointed basal cell, usually 3-septate, 16.2 to 24.2 × 3.2 to 4.0 μm. Microconidia were produced in false heads, oval, 0-septate, 6.2 to 8.2 × 3.3 to 3.8 μm, and the phialide was cylindrical. Chlamydospores were oval, 6.4 to 7.2 × 3.1 to 3.8 μm, alone produced in hypha. Morphological characteristics of the specimen were similar to the descriptions for Fusarium oxysporum (2). Genomic DNA of this isolate was extracted with a CTAB protocol (4) from mycelium and used as a template for amplification of the internal transcribed spacer (ITS) region of rDNA with primer pair ITS1/ITS4 (1). The full length of this sequence is 503 nt (GenBank Accession No. KJ009335), which exactly matched several sequences (e.g., JF807394.1, JX897002.1, and HQ451888.1) of F. oxysporum. Williams and Liu had listed F. oxysporum as the economically important pathogen of Hevea in Asia (3), while this is, to our knowledge, the first report of stem rot caused by F. oxysporum on rubber tree in China. References: (1) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (2) J. F. Leslie and B. A. Summerell. The Fusarium Laboratory Manual, 2006. (3) T. H. Williams and P. S. W. Liu. A host list of plant diseases in Sabah, Malaysia, 1976. (4) J. R. Xu et al. Genetics 143:175, 1996.

High revenues from rubber latex exports have led to a rapid expansion of commercial rubber cultivation and, as a consequence, the conversion of different land use types (e.g., natural forests) into rubber plantations, which may lead to a decrease in soil health. In this study in Quang Tri Province, Vietnam, we determined: (1) the variation of soil health parameters along a chronosequence of rubber tree stands and natural forests and (2) the relationships and potential feedback between vegetation types, vegetation structures and soil health. Our results revealed that: (1) soil health was higher in natural forests than in rubber plantations with a higher values in higher biomass forests; (2) soil health was lower in younger rubber plantations; (3) soil health depends on vegetation structure (with significantly positive relationships found between soil health and canopy cover, litter biomass, dry litter cover and ground vegetation cover). This study highlights the need for more rigorous land management practices and land use conversion policies in order to ensure the long-term conservation of soil health in rubber plantations.

Bark necrosis (BN), described and first studied in Côte d'Ivoire in the 1980s (2), affects most modern rubber plantations (i.e., grafted trees with high-yielding clones, intensive exploitation due to tapping frequency, and use of Ethrel as a yield stimulant) worldwide with a wide range of severity across sites. While previous (3) and recent (4) etiological analyses remain inconclusive, environmental factors were shown associated with BN. Numerous epidemiological surveys conducted in various African and Asian plantations on recently tapped blocks (less than 10-year-old trees) revealed the nonrandom location of the earliest single diseased trees. These risky areas are mainly characterized by the proximity of a swamp, plantation road, windrow, old bulldozer track, residual forest stump, or slope break. In BN emergence areas, while no significant correlation was found with chemical soil parameters, physical soil analyses (e.g., penetrometer) revealed higher soil compaction often associated with poorer rhizogenesis in BN trees (comparative root counts made in pits close to healthy or BN trees). Furthermore, initial BN symptoms were preferentially observed near the grafted bud at the rootstock/scion junction (RS/S). Numerous comparative ecophysiological measurements of leaf water potential, stem water potential, and predawn base potential using a plant moisture stress (PMS) pressure chamber indicated water stress in BN trees. These results and preliminary dye transfer studies at the RS/S junction suggested a nonoptimal vascular relation between the root system and the trunk of BN trees. In conclusion, compaction-associated reduced water availability of the soil and poor root capacity to meet the water demand during drier dry seasons combined with disturbed sap flows and recurrent local water drainage (latex flows) are now suspected to jointly act as the main exogenous causal stresses that induce the BN process at the RS/S bud zone before spreading upward to the tapping cut. This multidisciplinary approach gives a new comprehensive scenario for the emergence of this multifactorial physiological disease, now suspected to involve cyanide release (1) into the inner phloem of the rubber tree. References: (1) H. Chrestin. Plant Dis. 88:1047, 2004. (2) D. Nandris et al. Eur. J. For. Pathol. 21:325, 1991. (3) D. Nandris et al. Eur. J. For Pathol. 21:340, 1991. (4) F. Pellegrin et al. Plant Dis. 88:1046, 2004.

Knowing the stand age of rubber tree (Hevea brasiliensis) plantations is vitally important for best management practices, estimations of rubber latex yields, and carbon cycle studies (e.g., biomass, carbon pools, and fluxes). However, the stand age (as estimated from the establishment year of rubber plantation) is not available across large regions. In this study, we analyzed Landsat time series images from 1987–2015 and developed algorithms to identify (1) the establishment year of rubber plantations; and (2) the pre-conversion land cover types, such as old rubber plantations, evergreen forests, and cropland. Exposed soil during plantation establishment and linear increases in canopy closure during non-production periods (rubber seedling to mature plantation) were used to identify the establishment year of rubber plantations. Based on the rubber plantation map for 2015 (overall accuracy = 97%), and 1981 Landsat images since 1987, we mapped the establishment year of rubber plantations on Hainan Island (R2 = 0.85/0.99, and RMSE = 2.34/0.54 years at pixel/plantation scale). The results show that: (1) significant conversion of croplands and old rubber plantations to new rubber plantations has occurred substantially in the northwest and northern regions of Hainan Island since 2000, while old rubber plantations were mainly distributed in the southeastern inland strip; (2) the pattern of rubber plantation expansion since 1987 consisted of fragmented plantations from smallholders, and there was no tendency to expand towards a higher altitude and steep slope regions; (3) the largest land source for new rubber plantations since 1988 was old rubber plantations (1.26 × 105 ha), followed by cropland (0.95 × 105 ha), and evergreen forests (0.68 × 105 ha). The resultant algorithms and maps of establishment year and pre-conversion land cover types are likely to be useful in plantation management, and ecological assessments of rubber plantation expansion in China.

Land surface phenology derived from satellite data provides insights into vegetation responses to climate change. This method has overcome laborious and time-consuming manual ground observation methods. In this study, we assessed the influence of climate on phenological metrics of rubber (Hevea brasiliensis) in South Sumatra, Indonesia, between 2010 and 2019. We modelled rubber growth through the normalised difference vegetation index (NDVI), using eight-day surface reflectance images at 250 m spatial resolution, sourced from NASA’s Moderate Resolution Imaging Spectroradiometer (MODIS) Terra and Aqua satellites. The asymmetric Gaussian (AG) smoothing function was applied on the model in TIMESAT to extract three phenological metrics for each growing season: start of season (SOS), end of season (EOS), and length of season (LOS). We then analysed the effect of rainfall and temperature, which revealed that fluctuations in SOS and EOS are highly related to disturbances such as extreme rainfall and elevated temperature. Additionally, we observed inter-annual variations of SOS and EOS associated with rubber tree age and clonal variability within plantations. The 10-year monthly climate data showed a significant downward and upward trend for rainfall and temperature data, respectively. Temperature was identified as a significant factor modulating rubber phenology, where an increase in temperature of 1 °C advanced SOS by ~25 days and EOS by ~14 days. These results demonstrate the capability of remote sensing observations to monitor the effects of climate change on rubber phenology. This information can be used to improve rubber management by helping to identify critical timing for implementation of agronomic interventions.

Flowers of three Hevea brasiliensis clones, RRIM 527, RRIM 600 and GT 1, were analyzed under stereomicroscope and scanning electron microscope, aiming to observe hermaphroditism rates. Results showed 71.49% hermaphrodite flowers, 29.83% of which exhibited incompletely developed, residual anthers. The scanning electron microscope analysis did not detect differences in anther epidermis of male and bisexual flowers of RRIM 600 and RRIM 527. In GT 1 clone (sterile male), the anther epidermis was already weak at the beginning of floral development and completely wrinkled at the end of maturation. Consequently, the anthers were empty by this stage.

The main constraint in rubber plantations worldwide is the cessation of latex production because of two syndromes: (i) tapping panel dryness (TPD), a reversible physiological response to overexploitation; and (ii) bark necrosis (BN), an irreversible syndrome spreading from the collar toward the tapping cut. Early BN symptoms develop in the inner phloem tissues but never affect the cambium. Necrotic patches appear in the outer phloem, inducing bark cracking and peeling, but these alterations never lead to tree death. BN spreads gradually to neighboring rubber trees, and evidence of linear disease centers suggest that a pathogen may be involved, possibly transmitted by the tapping knife. Several previous etiological investigations (fungi, phytoplasma, bacteria, and virus) were performed (3) on leaves, bark, and latex using different methods (e.g., isolation, transmission, chemical treatments, and optic and electron microscope studies). Recent works focused on mechanically transmissible pathogens, such as viroid (2) or virus/double strand RNA, using RNA extraction (nonionic cellulose and appropriate ethanol concentrations) and treatment with RNase A, followed with sequential polyacrylamide gel electrophoresis (s-PAGE), reverse transcription-polymerase chain reaction (RT-PCR), degenerate oligonucleotide primer-PCR (DOP-PCR), and cloning and sequence analysis. While numerous viroid-like (between 250 and 400 nucleotides) and double strand virus-like (1,800 bp) low-molecular-weight RNAs were observed, no definite correlation was found with the BN status of trees. Sequencing of the various isolated RNAs only identified plasmids, nonpathogenic bacteria and yeasts, but none of the suspected pathogens. In addition, previous and recent transmission trials (tapping knife disinfection, bud grafting, bark implantation, and etc.) failed to confirm the involvement of a biotic agent. In conclusion, since these etiological investigations were inconclusive, a physiological disease is now suspected that involves exogenous stresses, nonoptimal vascular relations at the rootstock/scion junction and impaired cyanide metabolism (1,4). References: (1) H. Chrestin et al. Plant Dis. 88:1047, 2004. (2) N. Duran-Vila et al. J. Gen. Virol. 69:3069, 1988. (3) D. Nandris et al. Eur. J. For. Pathol. 21:340, 1991. (4) D. Nandris et al. Plant Dis. 88:1047, 2004.

In 1994 in the Sanggau/Sintang area in West Kalimantan province, most farmers relied mainly on jungle rubber, an old agroforestry system based on rubber seedling with low productivity, low establishment cost and low maintenance but high biomass and biodiversity. Most farmers at that period wanted to have access to clonal rubber planting material in order to improve their productivity (rubber clones do produce 3 times more than seedlings). The CIRAD/ICRAF/IRRI project called SRAP (Smallholder Rubber Agroforestry project) has set-up in 1994 on farm trials with 60 farmers in order to optimize clonal based new RAS according to local conditions and constraints. When SRAP started (1994/2007), the original objectives were multiple: i) to provide clone and high rubber productivity, ii) to maintain agroforestry practices to profit from positive externalities, and iii) to diversify income through timber, fruits, resins (Gaharu, Damar…) and other forest products (rattan, medicinal plants, forest vegetables etc). In 1997, came in the landscape oil palm estates though the very high and rapid development of private concessions. Oil palm became in the 2000’s the main priority for most smallholders. Today, all forest and most local jungle rubber have disappeared to the profit of roughly 2/3 of the area planted with oil palm (estates and smallholder) and 1/3 with clonal rubber for smallholder, either in monoculture or agroforestry. In 2019, CIFOR/FTA program funded a mission to CIRAD to obtain information about the evolution of RAS trials plots evolution in the province of West Kalimantan. The survey provide an idea of the historical and current trend in terms of local farming strategies concerning agroforestry. It raised also the question of clonal planting material availability for replanting and the poor tapping quality that lead to a reduction of the clonal rubber lifespan. Evolution of trials status over the period1994/2019 display the following results: i) Conversion to oil palm (20 %) or to clonal rubber monoculture (20 % mainly in Trimulia in Transdmigration area), ii) with agroforestry systems maintained in RAS 1 or 2 (50 %) and iii) evolution to tembawang at the end of rubber lifespan (10 %). We are back to the same problems faced in 1994: poor access to clonal planting material, no training on tapping frequency and practices but with some knowledge on clones and AF practices. The lessons learned are the following: i) Rubber agroforestry trials came right in time in 1994, with a strong demand from farmers, ii) but oil palm came in 1997 with a very strong pressure from concession companies providing a lucrative alternative to rubber cultivation with full credit (but loss of land) and better return to labor, iii) Interest in agroforestry practices remains high for old men but no interest is witnessed from younger generation, iv) It is now time for rubber replanting but the same old story remains: poor access to planting material), v) no good tapping practices, poor technical information available). These are essential to be able to maximize tree lifespan up to 35 years long.

When writing this botanical and historical review, it was necessary to get acquainted with the works of different authors from the XVII to the XXI century, written in Latin, old Spanish, old French, Spanish, French, German, Dutch, English and Russian. It shows the development of knowledge about growing on the American continents rubber-bearing trees, including their species definition and a botanical description. The main attention is paid to the Castilla elastica Cerv. from the Moraceae family, which served as a source of raw materials for the manufacture of an analogue of modern vulcanized rubber for ancient peoples of Mesoamerica and was then for many years the main rubber-bearing plant, whereas the priority have been given to Hevea brasiliensis (Wild. ex A. Juss.) Muell.-Arg. only after the beginning of plantation cultivation of this species in the end of XIX century. However, interest in Castilla did not completely disappear, and in the coming millennium, attempts were made to resume plantations. Sequencing of individual fragments of the nuclear and chloroplast genomes of Castilla species has also begun. The history of the appearance of the second erroneous name of this genus as Castilloa and the long process of returning the original name Castilla are described.

This research work was conducted to demonstrate the mechanism of white board marker ink production using locally sourced raw materials such as charcoal and used lube oil. In the production of the ink, the charcoal served as a pigment, used lube oil served as the primary binder or resin, ethanol served as solvent and gum Arabic served as an additive. The charcoal was obtained from processing of Mango, Oil bean (Ugba) and rubber trees, which were further crushed to their finest particles respectively and the used lube oil was obtained from mechanical engineering servicing unit of automobile engines. The crushed charcoal samples were characterized to determine the physio-chemical properties of some mineral elements such as Ca, Cu, P, K, C, S, N. however the mineral component that controlled the production of this ink was the Carbon content. The different ink samples were formulated in terms of odour, colour, hazardous reaction, pH, density and viscosity and compared with that of international standards. Results obtained showed a good match, indicating the reliability and the quality of the produced white board marker ink. The pH results for Ugba ink = 5.43, Rubber ink = 6.79, and Mango = 7.41. Empirical models were used to predict concentration with that of experimental values, a plot of concentration against time in terms of production yield revealed that the order of magnitude was rubber>Ugba>Mango whereas in terms of penetration and writing ability Ugba>rubber>mango. Furthermore, the research demonstrates the significance of the characteristics of the charcoal and the used lube oil in the quality of the end product. Finally, the research revealed that ink produced from the oil bean (ugba) charcoal and lube oil was best in terms of write-ability and quality in the production of white board marker ink.

The most basic and primary building material for construction of houses is the conventional brick. The rapid growth in today’s construction industry has obliged the civil engineers in searching for more efficient and durable alternatives far beyond the limitations of the conventional brick production [1-2]. A number of studies have been made and serious steps have been taken in manufacturing of bricks from several waste materials. However, the traditional mean of bricks production which has brought hazardous impacts to the context has not yet been changed or replaced by more efficient and sustainable one [3], [4]. Most of the researches went through enhancing the clay brick quality and properties by mixing the clay with various recycled wastes as foundry sand, granite sawing waste, harbour sediments, perlite, sugarcane, baggase ash, clay waste and fine waste of boron, sewage sludge, waste glass from structural wall and other different wastes. Compile this state of the art work of manufacturing bricks in the past and the current trend in the bricks industry with respect to the raw materials, ways of manufacturing and the out- comings.This project presents an experimental study on the utilization of waste materials which replaces clay with (Plastic covers, Ceramic Powder, Egg Shell Powder, GGBS, Silica Fume, Rice Husk Ash and Lime Powder) and Fine Aggregate with (Recycled glass, Dry Grass, Dead Leaves, Tree barks powder, Sugar cane powder, crumbed rubber) to produce eco-friendly Bricks. This project is an attempt to fill the gap of the past studies and suggest more sustainable and sophisticated methods of brick manufacturing in the future. 40 percent replacement of fine aggregate with crumbled rubber and dry grass in mortar bricks have given encouraging results, also the replacement of cement by egg shell powder at 20% has given a considerable result

The skeletal musculature is usually thought of as the primary organ of locomotion, and, like the tyres of a high-performance racing car, their composition, design, preparation and plasticity can make the difference between winner and ‘wannabe’. The similarities do not end there, however. Their primary components (cells of the mesodermal layer in the embryo and latex from the rubber tree) begin their existence in locations that can be quite distant from their final point of use and in forms that bear no resemblance to the final product. Their differentiation from primary material to final product entails extensive processing, and the integration of other materials and structures are essential to ensure their function. A fundamental difference, however, is that, in the case of muscle, once the embryo is formed, the progression from relatively undifferentiated mesodermal cells to the final structures is on autopilot, provided there are no contextual aberrations either from genetic or environmental causes. Our current understanding of how muscles develop is a synthesis of observations made on a wide array of organisms, including nematode worms, fruitflies, fish, frogs, birds and various mammals, as well as from the in vitro study of cells isolated from these species. The study of myogenesis in mammals, although less amenable to experimental manipulation, has been facilitated by the recent advances in mouse genetic engineering which has enabled the function of individual genes and cell types to be investigated, as well as the lineage of cells to be traced back to their origin. In this rapid trek through the life of a muscle, how the production of a mature functional muscle from its early inception is orchestrated will be outlined in exceedingly broad strokes so as to convey the wide range of processes that must be engaged in order to generate a functional muscle. Hopefully, enough information will be provided to encourage those interested to explore further.

This study uses data collected by ENSEA within the framework of the Agricultural Sector Support Project in Côte d'Ivoire (PSAC) carried out in 2015 to measure, firstly, the financial availability, use and quality of financial inclusion of farmers in the rural region of Sud-Comoé of Côte d'Ivoire and, secondly, to examine the factors that influence their choices in terms of financial inclusion. To this end, we construct indices for each of the three dimensions (access, use and quality) and a synthetic financial inclusion index. The results of the univariate analysis reveal that farmers in the zone are poorly financially included in terms of the "use" and "quality" dimensions, and that the overall financial inclusion situation of farmers in the Sud-Comoé is low (33% of farmers are financially included). To achieve the second objective, we used a logit model. The empirical results indicate that farmers with no schooling and fewer qualifications are more likely to be financially excluded; that the high cost of banking services and the low income of farmers limit their financial inclusion and, finally, that the experience acquired by farmers on the farm and the savings products offered by financial institutions are the factors that stimulate the financial inclusion of farmers. In addition, the education variable partly explains why financial inclusion is more frequent among rubber tree farmers than among oil palm farmers. In view of these results, economic choices and decisions must be targeted according to these empirical findings to increase the level of financial inclusion of producers, the true pillars of the economy of Côte d’Ivoire.

<p><strong>Abstract.</strong> Crop discrimination and acreage play a vital role in interpreting the cropping pattern, statistics of the produce and market value of each product. Sultan Battery is an area where a large amount of irrigated and rainfed paddy crops are grown along with Rubber, Arecanut and Coconut. In addition, the northern region of Sultan Battery is covered with evergreen and deciduous forest. In this study, the main objective is to evaluate the performance of optical and Synthetic Aperture Radar (SAR)-optical hybrid fusion imageries for crop discrimination in Sultan Bathery Taluk of Wayanad district in Kerala. Seven land use classes such as paddy, rubber, coconut, deciduous forest, evergreen forest, water bodies and others land use (e.g., built-up, barren etc.) were selected based on literature review and local land use classification policy. Both Sentinel-2A (optical) and sentinel-1A (SAR) satellite imageries of 2017 for Kharif season were used for classification using three machine learning classifiers such as Support Vector Machine (SVM), Random Forest (RF) and Classification and Regression Trees (CART). Further, the performance of these techniques was also compared in order to select the best classifier. In addition, spectral indices and textural matrices (NDVI, GLCM) were extracted from the image and best features were selected using the sequential feature selection approach. Thus, 10-fold cross-validation was employed for parameter tuning of such classifiers to select best hyperparameters to improve the classification accuracy. Finally, best features, best hyperparameters were used for final classification and accuracy assessment. The results show that SVM outperforms the RF and CART and similarly, Optical+SAR datasets outperforms the optical and SAR satellite imageries. This study is very supportive for the earth observation scientists to support promising guideline to the agricultural scientist, policy-makers and local government for sustainable agriculture practice.</p>

An experiment with the objective of evaluating the performance of 15 clones of rubber (Hevea brasiliensis) was conducted in the Konkan region of Western India. The clones under evaluation were RRII 5, RRII 6, RRII 105, RRII 208, RRII 308, RRIC 52, RRIC 100, RRIC 102, RRIC 105, RRIM 605, PB 260, PB 310, PB 311, PR 255, and PR 261. The region is a trial environment for the crop and experiences more than seven rainless months and severe drought in the summer months. Data on monthly girth growth w ere collected for six years from a trial with randomized block design. The growth of clones in terms of monthly girth increment growth (GIN, cm month−1) seasonal mean girth increments (MGIN, cm season−1) and mean relative increment rates (MRIR, mm cm−1 season−1) as well as annual MGIN (cm a−1) and MRIR (mm cm−1 a−1) was studied. Correlation analysis was performed to understand the effect of seasonal growth on the final growth. At the beginning of the study, the largest girth noted was for the clone RRII 6 (22. 5 cm) followed by RRII 208 (22.0 cm). PR 261 with a girth of only 14.2 cm was the least vigorous among the clones. A large portion of the growth occurred in the wet season only. During the dry season the growth rates of the clones declined substantially and decreases in girth ranging from 0.2 mm to 0.5 mm were noticed in most of the clones. At the end of the study period the largest girth observed was for clone RRII 208 (49.3 cm) and the lowest for PR 261 (39.3 cm). The highest proportion of tappable trees noted was for clone RRII 208 (52.4%) and the lowest for PR 261 (2.7%). The pooled average of tappable trees was on ly 17.5%. The data revealed that the immaturity period for Hevea in the region will not be less than 9 years under rainfed conditions. From the analysis based on the final girth it was concluded that clones RRII 208, RRIC 52, RRII 6, RRIC 100 and RRIC 102 are more tolerant to drought while RRII 105, RRIC 105, RRII 5, RRIM 605, PB 310, PB 260, PB 311, PR 255, RRII 308 and PR 261 are less tolerant. The results of correlation indicated that by analysing the growth, potentially drought-toleran t clones can be identified.

AbstractMonoculture cultivation system has impact on various biotic surrounding in it. Palm oil cultivation with intercropping system started to be popular to avoid environment damaged. Intercropping cultivation between palm oil and other plant was possible in existing both intraspecific and interspecific competation. Various form of this competation was needed to be learned. The compatible variables to measure these competation forms is Land Equal Ratio (LER) that able to compare the benefit between monoculture and multicultural cultivation system. The aim of this research was to determine the LER of multicultural cultivation between palm oli and rubber tree. this research had been held in February – July 2016 at Wanareja Village, Rimbo Ulu Sub-District, Tebo Regency. Survey method was use in this research, and the location above was purposely sampled suitable with the needed data. In the other word one reason of this location to be sampled was the only area that showed the existing of multicultural cultivation system especially between palm oil and rubber tree. There were several farmer characteristics to be sampled as a respondent e.g. : 1). Farmers that has no cooperation with enterprise ; 2). The farmer has at least one hectare of land; and 3). Show plant at the minimally age of 5 years. The result of this research showed that the production of Monoculture cultivation was higher than multicultural cultivation system, but it had 1.5 of LER so it indicated that the multicultural cultivation system give 50% higher benefit than Monoculture cultivation system. keywords: Monoculture, multicultural, Intercropping AbstrakPertanaman monokultur diyakini akan berdampak terhadap keragaman makhluk hidup yang berada pada areal tersebut. Wacana untuk membudidayakan sawit dengan sistem polikultur mulai mencuat seiring dengan kekhawatiran terjadinya kerusakan lingkungan. Budidaya polikultur antara tanaman sawit dengan tanaman lain memungkinkan terjadinya persaingan intra dan interspesifik. Perlu kajian untuk mengetahui bentuk dari persaingan tersebut. Peubah yang paling menentukan yang dapat diukur dari bentuk persaingan tersebut adalah Nisbah Kesetarahan Lahan (NKL) yang akan mengkaji keuntungan budidaya secara monokultur atau polikultur. Penelitian ini bertujuan untuk menentukan nilai nisbah kesetaraan lahan (NKL) pada tanaman polikultur kelapa sawit dengan tanaman karet. Penelitian telah dilaksanakan pada Bulan Pebruari sampai Juli 2016 di Desa Wanareja Kecamatan Rimbo Ulu Kabupaten Tebo. Penelitian menggunakan metode survey. Dalam hal ini pemilihan Kabupaten dan Desa dilakukan dengan sengaja ditentukan sesuai dengan kebutuhan data. Di Desa Wanareja Kecamatan Rimbo Ulu Kabupaten Tebo terdapat tanaman kelapa sawit polikultur dengan tanaman karet, maka petani dari desa ini yang dijadikan sampel. Adapun syarat-syarat petani yang akan dijadikan sampel adalah : 1) Petani yang tidak berkolaborasi dengan perusahaan, 2) luas lahan minimal satu hektar, dan 3) Umur tanaman minimal 5 tahun. Hasil penelitian menunjukkan produksi tanaman kelapa sawit sistem monokultur lebih tinggi dari sistem polikultur. Namun berdasarkan rata-rata produktivitas lahan perhitungan nilai nisbah kesetaraan lahan (NKL) menunjukkan nilai 1,5 ini menggambarkan bahwa pertanaman tumpangsari (polikultur) dari segi ekonomi lebih menguntungkan setengah atau 50% dari tanaman sistem monokultur. Kata Kunci : Monokultur, Polikultur, tumpangsari

Abstract Gopher tortoises (Gopherus polyphemus) occur in open-canopy pine habitat on well-drained soils in the southeastern United States, where they construct burrows that offer protection from thermal extremes, fire, and predators. Gopher tortoise populations have declined over the past 50 y, primarily as a result of habitat loss and degradation. Southeastern pine forests require active management with prescribed fire, mechanical thinning, or removal of hardwoods to maintain suitable habitat for gopher tortoises. In addition, many pine forests in the Southeast that support gopher tortoise populations are managed for multiple uses including intensive silviculture. Heavy equipment associated with these activities used in proximity to gopher tortoise burrows can cause them to collapse, potentially causing harm to tortoises or other imperiled organisms that use their burrows. Hence, there is a need for practical guidelines for use of heavy equipment for timber harvest, management, and other activities around gopher tortoise burrows to minimize risk to tortoises. We conducted a field study to determine the distance at which heavy equipment caused gopher tortoise burrows to collapse using a feller buncher, rubber-tire front-end loader, and an agricultural tractor with a tree-mower attachment in sandy clay loam (15 burrows) and undifferentiated deep sand (15 burrows) soils at a site in southwestern Georgia. All burrows were confirmed to be unoccupied by tortoises or other vertebrate commensal species using a camera scope before collapse. The greatest mean distance to collapse across all vehicles tested in sandy clay loam and undifferentiated deep sand was 2.19 ± 0.56 m and the maximum distance to collapse was 3 m. Given the variation in collapse distance, we recommend a buffer that extends 4 m in radius from the entrance of the gopher tortoise burrow to minimize risk of collapse from heavy equipment.

Moraines of two different age groups have been identified fronting the present-day glaciers and snowfields in Glacier National Park, Montana. The subdued, vegetated moraines of the older group have been found at 25 sites, mainly in the central part of the Lewis Range. These older moraines are in places overlain by the Mazama ash. Although the exact age of the moraines has not been determined by radiocarbon dating, vegetative evidence and correlation with other pre-altithermal age moraines in the Rocky Mountains suggest that these older moraines date from 10 000 BP or earlier. Whether these moraines are the product of a separate advance after the end of the Wisconsin glaciation or are simply the product of the last advance or stillstand of Wisconsin glaciers before final deglaciation is not known.Moraines of the younger group, consisting of fresh bouldery rubble, are common throughout Glacier Park. Tree-ring analyses indicate that some of these younger moraines were deposited by advances that culminated during the mid-19th century. At that time there were more than 150 glaciers in Glacier Park. This episode of mid-19th century climatic cooling resulted in the most extensive glacial advance in this region since the end of the Wisconsin glaciation.Present-day glaciers have shrunk drastically from their mid-19th century positions; more than half the glaciers present during that time no longer exist. Much of this retreat occurred between 1920 and the mid-1940's, corresponding to a period of above-average summer temperatures and below-average annual precipitation in this region. Between 1966 and 1979, several of the larger glaciers in the Mount Jackson area of Glacier Park advanced as much as 100 m.

The Humogo Waterfall Tourism Area is one of the waterfall tours located on Nias Island, Fadoro Village, Gunungsitoli Idanoi Subdistrict, Gunungsitoli City, North Sumatra Province. The plan for structuring the Humogo Waterfall Tourism Area is planned based on the Regional Work Plan of the Gunungsitoli City Tourism and Culture Office in 2018, where the arrangement is aimed at adding facilities and infrastructure to support tourism in the Humogo Waterfall Tourism Area and the preparation of the Humogo Waterfall Tourism Area Master Plan, Gunungsitoli city. The Humogo Waterfall area is located in Fadoro Village with an area of 15.5 ha with most of the area still dominated by forests and plantations. The Humogo Waterfall Tourism Area is one of the leading tourist destinations in Gunungsitoli City, which was only opened to the public at the end of 2018. This waterfall has a distinctive characteristic of a multilevel waterfall so it is very interesting to be explored by tourists and has very natural conditions that are still very natural surrounded by trees such as coconut trees, rubber trees and so on. However, this tourism area still has shortcomings in the form of supporting tourism facilities and infrastructure so it is necessary to propose facilities and infrastructure in order to increase the tourist attraction of Humogo Waterfall. The analysis carried out is a policy analysis, site and site analysis, best practice analysis, tourist attraction analysis, perceptions and preferences analysis and analysis of spatial requirements so as to produce proposals and the Humogo Waterfall Tourism Area Planning Plan which is laid out with the concept of ecotourism Keywords: ecotourism; nature tourism; tourist zoning; waterfall tourism region AbstrakKawasan Pariwisata Air Terjun Humogo merupakan salah salah satu wisata air terjun yang terletak di Pulau Nias, tepatnya di Desa Fadoro, Kecamatan Gunungsitoli Idanoi, Kota Gunungsitoli, Provinsi Sumatera Utara. Rencana penataan Kawasan Pariwisata Air Terjun Humogo direncanakan berdasarkan Rencana Kerja Perangkat Daerah Dinas Pariwisata dan Kebudayaan Kota Gunungsitoli Tahun 2018, dimana penataan yang dilakukan bertujuan untuk menambah fasilitas dan prasarana yang mendukung pariwisata di Kawasan Pariwisata Air Terjun Humogo dan penyusunan Masterplan Kawasan Pariwisata Air Terjun Humogo, Kota Gunungsitoli. Kawasan Air Terjun Humogo terletak di Desa Fadoro dengan luas 15,5 ha dengan sebagian besar kawasan masih didominasi oleh hutan dan perkebunan. Kawasan Pariwisata Air Terjun Humogo merupakan salah satu destinasi wisata unggulan di Kota Gunungsitoli yang baru dibuka untuk umum pada akhir tahun 2018. Air terjun ini memiliki ciri khas yaitu air terjun yang bertingkat sehingga sangat menarik untuk dijelajahi oleh wisatawan serta memiliki kondisi alam yang masih sangat alami yang dikelilingi oleh pepohonan seperti pohon kelapa, pohon karet dan lain sebagainya. Namun area wisata ini masih memiliki kekurangan berupa sarana dan prasarana penunjang wisata sehingga diperlukan usulan fasilitas dan prasarana agar dapat meningkatkan daya tarik wisata Air Terjun Humogo. Analisis yang dilakukan adalah analisis kebijakan, analisis lokasi dan tapak, analisis best practice, analisis daya tarik wisata, analisis persepsi dan preferensi serta analisis kebutuhan ruang sehingga menghasilkan usulan dan Rencana Penataan Kawasan Pariwisata Air Terjun Humogo yang ditata dengan konsep ekowisata.

<p>ABSTRAK</p><p>Ampas sagu berpotensi sebagai sumber bahan organik untukmeningkatkan kesuburan tanah. Pengaruh ampas sagu terhadap kesuburantanah ditentukan oleh tingkat dekomposisi dan komposisinya. Penelitianini bertujuan untuk mengetahui tingkat dekomposisi ampas sagu terhadappertumbuhan dan produksi lada perdu. Penelitian dilaksanakan di KebunPercobaan Institut Pertanian Bogor dan Balai Penelitian Tanaman Rempahdan Obat pada bulan Mei 2003 sampai April 2004. Tanaman yangdigunakan adalah tanaman lada perdu umur 4 tahun yang ditanam dibawah tanaman karet. Percobaan menggunakan rancangan acak kelompok(RAK), dengan 3 ulangan dan 6 tan/perlakuan yang menguji perlakuankombinasi antara tingkat dekomposisi ampas sagu(W) dan komposisinyadengan kompos (A), terdiri dari : W 0 = ampas sagu dekomposisi 0 bulan,W 1 = ampas sagu dekomposisi 1 bulan, dan W 2 = ampas sagu dekomposisi2 bulan dan A 1 = 100% ampas sagu, A 2 = 75% ampas sagu + 25% kompos,A 3 = 50% ampas sagu + 50% kompos dan A 4 = 25% ampas sagu + 75%kompos. Hasil penelitian menunjukkan bahwa penggunaan mulsa ampassagu 100 % dalam bentuk segar dekomposisi 1 bulan ternyata menghambatpertambahan jumlah cabang tersier lada perdu hingga akhir penelitian.Dibandingkan dengan kontrol terjadi perbedaan pengaruh yang nyataterhadap komponen produksi, sebagai respon terhadap kombinasiperlakuanW 2 A 2 , W 2 A 1, W 1 A 3 pada panjang tandan (9,13; 9,03; 8,70 cm),dan W 2 A 2 , W 2 A 1, W 0 A 4 pada jumlah biji/tandan (46,67; 43,00; 41,73biji/tandan), serta W 2 A 2 , W 2 A 1, W 2 A 3 pada bobot kering buahlada/tanaman (323,20; 314,90; 297,85 g/tanaman). Pemberian ampas sagu75% + 25% kompos (W 2 A 2 ) dan 100% (W 2 A 1 ) dekomposisi 2 bulanmampu meningkatkan jumlah biji 91 - 107% dan menghasilkan bobotkering buah yang tinggi sebesar 323,20 dan 314,90 g per tanaman.</p><p>Kata kunci: Ampas sagu, mulsa, lada perdu, produktivitas</p><p>ABSTRACT</p><p>Use of Sago Waste and Compost to Increase theProductivity of Bushy Black Pepper</p><p>As a source of organic matter to improve soil fertility, sago wastecan also be used as an ameliorant and natural herbicide. The effect of sagoand compost on soil fertility is determined by the grade of decompositionand its compositions. An experiment was conducted at the experimentalgarden of Bogor Agricultural University Bogor and Indonesian Medicinaland Aromatic Crops Research Institute, from May 2003 to April 2004.Plant material used was bushy black pepper of the Petaling variety, 4 yearsold, planted under rubber trees. The treatments used were A 1 W 0 = 100%sago waste without decomposition; A 1 W 1 = 100% sago waste after 1month decomposition; A 1 W 2 = 100% sago waste after 2 monthsdecomposition; A 2 W 0 = 75% sago waste + 25% compost, withoutdecomposition; A 2 W 1 = 75% sago waste + 25% compost, after 1 monthdecomposition; A 2 W 2 = 75% sago waste + 25% compost, after 2 monthsdecomposition; A 3 W 0 = 50% sago waste + 50% compost, withoutdecomposition; A 3 W 1 = 50% sago waste + 50% compost, after 1 monthdecomposition; A 3 W 2 = 50% sago waste + 50% compost, after 2 monthsdecomposition; A 4 W 0 = 25% sago waste + 75% compost, withoutdecomposition; A 4 W 1 = 25% sago waste + 75% compost, after 1 monthdecomposition; A 4 W 2 = 25% sago waste + 75% compost, after 2 monthsdecomposition; TBO = without organic matter (control). The experimentwas performed with a randomized block design, with 3 replicates and 6plants/plot. The results showed, that 100% sago palm waste after 1 monthdecomposition hampered number of tertiary branch until the end ofresearch. The real difference on the productivity components as a respondof combination sago waste treatments wereW 2 A 2 , W 2 A 1, W 1 A 3 for lengthof stem (9.13; 9.03; 8.70 cm), and W 2 A 2 , W 2 A 1, W 0 A 4 for number ofberries/spike (46.67; 43.00; 41.73 kernels/spike), and W 2 A 2 , W 2 A 1, W 2 A 3for dry weight of berries/plant (323.20; 314.90; 297.85 g/plant).Extension of 75% sago palm waste+ 25% compost (W 2 A 2 ) and 100%(W 2 A 1 ) after 2 months decomposition were able to increase amount ofseed 91 - 107 % and dry weight of berries /plant which were323.20 and314.90 g per plant.</p><p>Key words: Sago waste, mulch, bushy pepper black, productivity</p>

Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. 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ABSTRACT The use of the Ethrel® stimulant on rubber cultivation has become essential to ensure greater rubber production. When applied on the cut of the tree, this stimulant releases the ethylene gas that causes an increase of the exudation period of the latex flow. Dilutions and applications should be performed correctly so that they do not cause tapping panel dryness, which would leave the plant unproductive for a long time. Thus, the objective of this study was to evaluate the effects of increasing doses of ethrel on tapping panel dryness and rubber production of rubber tree clones. Three experiments were conducted using a randomized block design with four treatments (ethrel doses) and three replicates. The clones used were RRIM600, PR255 and PB217 submitted to doses of the stimulant Ethrel 0%, 2.5%, 3.3% and 5% with an interval of application of 30 days. The d/7 bleeding system was used. The productive performance was evaluated monthly and the total of plants with tapping panel dryness at the end of the work. All clones presented a linear positive response to increasing doses. The dose of ethrel 5% favors higher rubber yields in the three evaluated clones, but is associated with high percentage of drying. Thus, it was concluded that the production of rubber is affected positively with the increase of ethrel doses, but this increase causes a higher incidence of panel drying. Thus, it is recommended to use the ethrel 2.5% dose so the production is guaranteed without causing the tapping panel dryness.

Изучение роста и продуктивности гевеи бразильской проводилось на про- мышленных плантациях в районе Тхонг Нхат (провинция Донг Най, Вьетнам). Плантации закладывались привитыми саженцами пяти клонов – GT1, PB260, PB235, RRIC121, VM515 на площадях, имеющих уклон менее 3. В качестве подвоя использовали сеянцы гевеи бразильской, выращенные в полиэтиленовых тубах. Посадка проводилась в ямки, подготовленные вручную. Перед посадкой в ямку вносился комплекс органических и минеральных удобрений. Перед посад- кой снималась пленка с корнезакрывающего кома, растения помещались в ямку и заделывались почвой, смешанной с органическими и минеральными удобре- ниями. Первоначальная густота посадки на всех вариантах исследования состав- ляла 560 шт./га. Уход за посадками заключался в многократном удалении травя- нистых растений вокруг посаженного растения. Диаметр обрабатываемой площади увеличивался до размера проекции кроны. После достижения расте- ниями высоты 3 м начинали постепенную обрезку нижних ветвей, доводя высо- ту ствола без нижних ветвей до 2,5–3,3 м. Для определения хода роста заклады- вались постоянные пробные площади на плантациях гевеи бразильской в возрасте 1, 5, 7, 10, 15, 20, 25, 30 лет размером 25  25 м, на которых проводился учет биометрических показателей всех деревьев. Определение продуктивности латекса проводили на трех деревьях на тех же постоянных пробных площадях в течение 30 дней по каждому клону в возрасте 7, 10, 15, 20, 25, 30 лет. Сбор ла- текса производился ежедневно. Массу латекса определяли взвешиванием каждой пробы по отдельному дереву в г/дер./сут. На плантациях все клоны имеют высо- кую приживаемость и сохранность, стабильно высокий прирост по высоте и диаметру ствола и высокую продуктивность млечного сока. Клон RRIC121 пока- зывает более высокие темпы роста уже с пятилетнего возраста и это преимуще- ство сохраняется до конца эксплуатации плантаций в 30-летнем возрасте как по биометрическим показателям, так и по продуктивности млечного сока. Биомет- рические параметры и динамика запаса древесины у клонов GT1, PB235 и VM515 не имеют достоверных отличий друг от друга, но несколько ниже, чем у клона RRIC121. Запас древесины у клона PB260 уже в 10-летнем возрасте ниже, чем у остальных клонов, и остается таковым на протяжении всего срока наблю- дений. Хотя промышленная заготовка млечного сока на плантация гевеи бра- зильской во Вьетнаме проводится с 7 и до 30-летнего возраста, наибольшая про- дуктивность у всех изучаемых клонов наступает с 10 и до 25-летнего возраста, а максимальная продуктивность – в 15–20-летнем возрасте. Лидирующие позиции по продуктивности млечного сока в период максимальной продуктивности име- ет клон RRIC121, несколько ниже продуктивность у клонов PB235 и VM515. Самая низкая продуктивность отмечена у клонов GT1 и PB235. Наиболее высо- кая корреляция между продуктивностью млечного сока и биометрическими по- казателями гевеи бразильской отмечена для диаметра у корневой шейки, протя- женности кроны и объема ствола отдельных деревьев. На увеличение этих показателей отдельных деревьев должны быть направлены агротехнические ме- роприятия при создании и эксплуатации плантаций гевеи бразильской. The growth and productivity of Brazilian Hevea (Hevea brasiliensis) on commer- cial plantations in the Thong Nhat district, Dong Nai province, Vietnam, are studied and analyzed. The plantations were established with the grafted seedlings of five clones: GT1, PB260, PB235, RRIC121, and VM515, in the areas with a ground surface slope of less than 30°. The Brazilian Hevea seedlings grown in polyethylene tubes were used as rootstock. The seedlings were planted into pits prepared manually. Be- fore planting, a complex of organic and mineral fertilizers was introduced into the pits. The polyfilm was removed from the rootballs, and then the plants were placed into pits and embedded with soil mixed with organic and mineral fertilizers. The initial planting density was 560 plants per ha for all variants in this research. Tending consisted in re- peated weeding of herbaceous plants in the vicinity of the seedlings. The diameter of the cultivated area was increased to the size of the crown projection. After the trees reached 3 meters in height, lower branches were successively pruned so that the trees could not have any branches up to 2.5–3.3 m in height. To determine the growth rate, permanent trial plots were established on the Brazilian Hevea plantations at the age of 1, 5, 7, 10, 15, 20, 25, and 30, each 25 x 25 m, on which the biometric parameters of all trees were measured. Latex productivity was determined for 3 trees on the same permanent trial plots during 30 days for each clone at the age of 7, 10, 15, 20, 25, and 30. Latex was collected every day. The latex mass was determined by weighing each sample from a separate tree in grams every day. On the plantations, all clones show high survival and safety, stable high growth in height and diameter, and a high produc- tivity of latex. Clone RRIC121 has been showing a higher growth rate since the age of five and this advantage shows itself until the end of the plantation exploitation at the age of 30 both in biometric parameters and latex productivity. Biometric parameters and dynamics of wood stock in clones GT1, PB235 and VM515 have no significant differences from each other, but they are somewhat lower than those of clone RRIC121. Already at the age of 10, the wood stock of clone PB260 was smaller as compared with that of the other clones and it has not changed over the entire observa- tion period. Although on the Brazilian Hevea plantations in Vietnam, latex is collected in production quantities from the trees aged from 7 to 30, the highest productivity of all the clones under study is shown by the trees within the age period from 10 to 25, and the maximum productivity, at the age from 15 to 20. Clone RRIC121 has the lead- ing positions in latex productivity in the maximum productivity period; somewhat lower productivity is shown by clones PB235 and VM515. Clones GT1 and PB235 have the lowest productivity. The highest relationship between the lateх productivity and the biometric indicators of Brazilian Hevea is recorded for the root neck diameter, the crown length, and the trunk volume of individual trees. Special agrotechnical measures should be developed and applied during the establishment and management of Brazilian Hevea plantations in order to improve these parameters.

ABSTRACT The use of the Hevea brasiliensis tree in latex rubber production has resulted in a consolidated role of this tree in the Brazilian economy. However, at the end of the productive cycle of the tree that lasts for approximately 25 years, the timber resulting from this venture has no added value and is usually used for the production of firewood. In order to introduce this species into the furniture and civil construction industry, the current study aimed to evaluate the quality of particleboard produced by adding Pinus oocarpa wood with Hevea brasiliensis wood in different ratios. The particleboards were produced with a nominal density of 0.60 g/cm³ and 8% (wt%) urea-formaldehyde resin and pressed at 160 °C for 8 min at a pressure of 4.0 MPa. The quality of the particleboard was evaluated by water absorption and thickness swelling, internal bonding, static bending, and screw withdrawal tests. The substitution of Pinus particles by Hevea wood resulted in decreased water absorption and thickness swelling. The boards produced with a higher proportion of Hevea had better dimensional stability due to the greater porosity of the Pinus oocarpa particles. The inclusion of Hevea brasiliensis wood resulted in a 75% increase in the modulus of rupture and a 300% increase in internal bonding compared with the particleboards produced only with Pinus oocarpa wood. The results show that Hevea brasiliensis wood could be used to obtain higher value-added products and contribute to the diversification of the raw materials used in the particleboard industries of Brazil.

<em>Peat land accumulates organic materials and emits greenhouse gas (GHG). Agricultural activities in peat land cause the subsidence of peat land surface and the loss of carbon in the form of GHG. Appropriate management of peat land for agriculture would reduce GHG emission. This research aims to understand the microorganism population dynamics and emission of GHG on the treatment of chicken manure application in peat land. The research was conducted in the GHG Laboratory of Indonesia Agricultural Environment Research Institute (IAERI) in 2012 using peat material taken from Jabiren, Central Kalimantan. The experiment was done by incubating peat soil for 2 months with the treatment of chicken manure application and without manure. The incubation experiment was conducted by using paralon PVC pipe. Parameters observed included pH, Eh, bacteria population and fungi, as well as GHG flux (CO₂, CH₄and N₂O). GHG samples taking method was used a sealed containment. The research result showed at the peat given chicken manure treatment, bacteria population decreased at the end of incubation; fungi population, however, increased. The application of chicken manure on peat land planted rubber trees and pineapples would reduce GHG emission by 12.8% as compared without manure application.</em>

Apricot vein clearing-associated virus is the type species of genus Prunevirus, family Betaflexiviridae. The virus was first discovered from an Italian apricot tree (Prunus armeniaca) showing leaf vein clearing and mottling symptoms (Elbeaino et al. 2014). Since then, apricot vein clearing-associated virus (AVCaV) has been reported in symptomatic and asymptomatic plants from other countries (Marais et al. 2015; Kinoti et al. 2017; Kubaa et al. 2014). In 2018, a domestic selection of a flowering apricot (P. mume cv. Peggy Clarke) (PC01) with no discernible foliar virus-like symptoms was received for inclusion in the Foundation Plant Services (UC-Davis) collection. The plant originated from a private Prunus collection located in California. Total nucleic acids (TNA) were isolated from PC01 leaves using MagMax Plant RNA Isolation Kit (Thermo Fisher Scientific). The TNA were analyzed for a panel of 15 Prunus-infecting viruses by reverse-transcription quantitative PCR (RT-qPCR) (Diaz-Lara et al. 2020). In addition, to screen for sap-transmissible viruses, young leaves of PC01 were homogenized in inoculation buffer and were rubbed onto leaves of herbaceous indicator plants, Chenopodium amaranticolor, C. quinoa, Cucumis sativus, and Nicotiana clevelandii (Rowhani et al. 2005). The source PC01 tested negative for the 15 screened viruses. Interestingly, vein clearing symptoms were observed on leaves of C. quinoa and C. amaranticolor plants (Figure S1). These results suggested the presence of a mechanically transmissible virus in PC01. To determine the identity of mechanically transmissible viral agent, symptomatic C. quinoa and PC01 plant were advanced for high throughput sequencing analysis. Aliquots of TNA from PC01 and C. quinoa were rRNA-depleted and used for cDNA library preparation with TruSeq Stranded Total RNA kit (Illumina). The raw reads were trimmed, de novo assembled, and subsequently were annotated using tBLASTx algorithm (Al Rwahnih et al. 2018). A total of 47,261,138 and 8,812,296 single-end reads were obtained from cDNA libraries of PC01 and C. quinoa, respectively. The de novo assembly generated near-complete contigs resembling AVCaV genome ) from both PC01 and C. quinoa, which were 99.8% identical at the nucleotide level. The longest contig (8,342 nucleotides, 73.5x coverage depth) obtained from PC01 was further completed using SMARTer RACE 5’/3’ kit (Takara Bio). The complete genome sequence of AVCaV-PC01 is 8,364 nucleotides long (GenBank: MK170158). The full-length virus genome was compared with GenBank database using BLASTn, which the best hit corresponded to KY132099 with 98% identity. Additionally, AVCaV infection was confirmed in both PC01 selection and the symptomatic C. quinoa by RT-PCR as previously described (Marais et al. 2015). Lastly, symptomatic leaves of C. quinoa were used in leaf dip method to visualize virus particles by transmission electron microscope. As a result, flexuous rod-shaped virions were observed from leaf dips of symptomatic C. quinoa plants (Figure S2). Therefore, our results represent the first report of AVCaV in California, USA. Furthermore, mechanical transmission of an AVCaV isolate infecting flowering apricot to herbaceous hosts was confirmed. Field surveys and biological studies are underway to determine the prevalence of AVCaV in commercial orchards and assess its effect on tree performance.

The German anatomist and artist Gunther von Hagens’s exhibition Body Worlds has toured Europe, Asia and the US several times, provoking both interest and dismay, fascination and disgust. This “original exhibition of real human bodies” features whole cadavers as well as specific body parts and it is organized thematically around specific bodily functions such as the respiratory system, blood circulation, skeletal materials and brain and nervous system. In each segment of the exhibition these themes are illustrated using parts of the body, presented in glass cases that are associated with each function. Next to these cases are the full body cadavers—the so-called “plastinates”. The Body Worlds exhibition is all about perception-in-motion: it is about circumnavigating bodies, stopping in front of a plastinate and in-corporating it, leaning over an arm or reaching towards a face, pointing towards a discrete blood vessel, drawing an abstract line between two organs. Experiencing here is above all a matter of reaching-towards and incorporeally touching bodies (Manning, Politics of Touch). These bodies are dead, still, motionless, “frozen in time between death and decay” (von Hagens, Body Worlds). Dead and still eerily animate, just as the surface of a freeze-frame photograph would seem to capture spatially a movement in its unfolding becoming, plastinates do not simply appear as dead matter used to represent vitality, but rather [...] as persons who managed to survive together with their bodies. What “inner quality” makes them appear alive? In what way is someone present, when what is conserved is not opinions (in writing), actions (in stories) or voice (on tape) but the body? (Hirschauer 41—42) Through the corporeal transformation—the plastination process—that these bodies have gone through, and the designed space of the exhibition—a space that makes possible both innovative and restrictive movements—these seemingly dead bodies come alive. There is a movement within these bodies, a movement that resonates with-in the exhibition space and mobilises visitors.Two ways of thinking movement in relation to stillness come out of this. The first one is concerned with the ordering and designing of space by means of visual cues, things or texts. This relates to stillness and slowness as suggestive, imposed and enforced upon bodies so that the possibilities of movement are reduced due to the way an environment is designed. Think for example of the way that an escalator moulds movements and speeds, or how signs such as “No walking on the grass” suggest a given pattern of walking. The second one is concerned with how movement is linked up with and implies continuous change. If a body’s movement and exaltation is reduced or slowed down, does the body then become immobile and still? Take ice, water and steam: these states give expression to three different attributes or conditions of what is considered to be one and the same chemical body. But in the transformation from one to the other, there is also an incorporeal transformation related to the possibilities of movement and change—between motion and rest—of what a body can do (Deleuze, Spinoza).Slowing Down Ever since the first exhibition Body Worlds has been under attack from critics, ethicists, journalists and religious groups, who claim that the public exhibition of dead bodies should, for various reasons, be banned. In 2004, in response to such criticism, the Californian Science Centre commissioned an ethical review of the exhibition before taking the decision on whether and how to host Body Worlds. One of the more interesting points in this review was the proposition that “the exhibition is powerful, and guests need time to acclimate themselves” (6). As a consequence, it was suggested that the Science Center arrange an entrance that would “slow people down and foster a reverential and respectful mood” (5). The exhibition space was to be organized in such a way that skeletons, historical contexts and images would be placed in the beginning of the exhibition, the whole body plastinates should only be introduced later in the exhibition. Before my first visit to the exhibition, I wasn’t sure how I would react when confronted with these dead bodies. To be perfectly honest, the moments before entering, I panicked. Crossing the asphalt between the Manchester Museum of Science and the exhibition hall, I felt dizzy; heart pounding in my chest and a sensation of nausea spreading throughout my body. Ascending a staircase that would take me to the entrance, located on the third floor in the exhibition hall, I thought I had detected an odour—rotten flesh or foul meat mixed with chemicals. Upon entering I was greeted by a young man to whom I presented my ticket. Without knowing in advance that this first room had been structured in such a way as to “slow people down”, I immediately felt relieved as I realized that the previously detected smell must have been psychosomatic: the room was perfectly odourless and the atmosphere was calm and tempered. Dimmed lights and pointed spotlights filled the space with an inviting and warm ambience. Images and texts on death and anatomical art were spread over the walls and in the back corners of the room two skeletons had been placed. Two glass cases containing bones and tendons had been placed in the middle of the room and next to these a case with a whole body, positioned upright in ‘anatomically correct’ position with arms, hands and legs down. There was nothing gruesome or spectacular about this room; I had visited anatomical collections, such as that of the Hunterian Museum in London or Medical Museion in Copenhagen, which in comparison far surpassed the alleged gruesomeness and voyeurism. And so I realized that the room had effectively slowed me down as my initial state of exaltation had been altered and stalled by the relative familiarity of images, texts and bare bones, all presented in a tempered and respectful way.Visitors are slowed down, but they are not still. There is no degree zero of movement, only different relations of speeds and slowness. Here I think it is useful to think of movement and change as it is expressed in Henri Bergson’s writings on temporality. Bergson frequently argued that the problem of Western metaphysics had been to spatialise movement, as in the famous example with Zeno’s arrow that—given that we think of movement as spatial—never reaches the tree towards which it has been shot. Bergson however did not refute the importance and practical dimensions of thinking through immobility; rather, immobility is the “prerequisite for our action” (Creative Mind 120). The problem occurs when we think away movement on behalf of that which we think of as still or immobile.We need immobility, and the more we succeed in imagining movement as coinciding with the immobilities of the points of space through which it passes, the better we think we understand it. To tell the truth, there never is real immobility, if we understand by that an absence of movement. Movement is reality itself (Bergson, Creative Mind 119).This notion of movement as primary, and immobility as secondary, gives expression to the proposition that immobility, solids and stillness are not given but have to be achieved. This can be done in several ways: external forces that act upon a body and transform it, as when water crystallizes into ice; certain therapeutic practices—yoga or relaxation exercises—that focus and concentrate attention and perception; spatial and architectural designs such as museums, art galleries or churches that induce and invoke certain moods and slow people down. Obviously there are other kinds of situations when bodies become excited and start moving more rapidly. Such situations could be, to name a few, when water starts to boil; when people use drugs like nicotine or caffeine in order to heighten alertness; or when bodies occupy spaces where movement is amplified by means of increased sensual stimuli, for example in the extreme conditions that characterize a natural catastrophe or a war.Speeding Up After the Body Worlds visitor had been slowed down and acclimatised in and through the first room, the full body plastinates were introduced. These bodies laid bare muscles, tissues, nerves, brain, heart, kidneys, and lungs. Some of these were “exploded views” of the body—in these, the body and its parts have been separated and drawn out from the position that they occupy in the living body, in some cases resulting in two discrete plastinates—e.g. one skeleton and one muscle-plastinate—that come from the same anatomical body. Congruent with the renaissance anatomical art of Vesalius, all plastinates are positioned in lifelike poses (Benthien, Skin). Some are placed inside a protective glass case while others are either standing, lying on the ground or hanging from the ceiling.As the exhibition unfolds, the plastinates themselves wipe away the calmness and stillness intended with the spatial design. Whereas a skeleton seems mute and dumb these plastinates come alive as visitors circle and navigate between them. Most visitors would merely point and whisper, some would reach towards and lean over a plastinate. Others however noticed that jumping up and down created a resonating effect in the plastinates so that a plastinate’s hand, leg or arm moved. At times the rooms were literally filled with hordes of excited and energized school children. Then the exhibition space was overtaken with laughter, loud voices, running feet, comments about the gruesome von Hagens and repeated remarks on the plastinates’ genitalia. The former mood of respectfulness and reverence has been replaced by the fascinating and idiosyncratic presence of animated and still, plastinated bodies. Animated and still? So what is a plastinate?Movement and Form Through plastination, the body undergoes a radical and irreversible transformation which turns the organic body into an “inorganic organism”, a hybrid of plastic and flesh (Hirschauer 36). Before this happens however the living body has to face another phase of transition by which it turns into a dead cadaver. From the point of view of an individual body that lives, breathes and evolves, this transformation implies turning into a decomposing and rotting piece of flesh, tissue and bones. Any corpse will sooner or later turn into something else, ashes, dust or earth. This process can be slowed down using various techniques and chemicals such as mummification or formaldehyde, but this will merely slow down the process of decomposition, and not terminate it.The plastination technique is rather different in several respects. Firstly the specimen is soaked in acetone and the liquids in the corpse—water and fat—are displaced. This displacement prepares the specimen for the next step in the process which is the forced vacuum impregnation. Here the specimen is placed in a polymer mixture with silicone rubber or epoxy resin. This process is undertaken in vacuum which allows for the plastic to enter each and every cell of the specimen, thus replacing the acetone (von Hagens, Body Worlds). Later on, when this transformation has finished, the specimen is modelled according to a concept, a “gestalt plastinate”, such as “the runner”, “the badminton player” or “the skin man”. The concept expresses a dynamic and life-like pose—referred to as the gestalt—that exceeds the individual parts of which it is formed. This would suggest that form is in itself immobility and that perception is what is needed to make form mobile; as gestalt the plastinated body is spatially immobilised, yet it gives birth to a body that comes alive in perception-movement. Once again I think that Bergson could help us to think through this relation, a relation that is conceived here as a difference between form-as-stillness and formation-as-movement:Life is an evolution. We concentrate a period of this evolution in a stable view which we call a form, and, when the change has become considerable enough to overcome the fortunate inertia of our perception, we say that the body has changed its form. But in reality the body is changing form at every moment; or rather, there is no form, since form is immobile and the reality is movement. What is real is the continual change of form; form is only a snapshot view of a transition (Bergson, Creative Evolution 328, emphasis in original).In other words there is a form that is relative to human perception, but there is “underneath” this form nothing but a continuous formation or becoming as Bergson would have it. For our purposes the formation of the gestalt plastinate is an achievement that makes perceptible the possibility of divergent or co-existent durations; the plastinate belongs to a temporal rhythm that even though it coincides with ours is not identical to it.Movement and Trans-formation So what kind of a strange entity is it that emerges out of this transformation, through which organic materials are partly replaced with plastic? Compared with a living body or a mourned cadaver, it is first and foremost an entity that no longer is subject to the continuous evolution of time. In this sense the plastinate is similar to cryogenetical bodies (Doyle, Wetwares), or to Ötzi the ice man (Spindler, Man in the ice)—bodies that resist the temporal logic according to which things are in constant motion. The processes of composition and decomposition that every living organism undergoes at every instant have been radically interrupted.However, plastinates are not forever fixed, motionless and eternally enduring objects. As Walter points out, plastinated cadavers are expected to “remain stable” for approximately 4000 years (606). Thus, the plastinate has become solidified and stabilized according to a different pattern of duration than that of the decaying human body. There is a tension here between permanence and change, between bodies that endure and a body that decomposes. Maybe as when summer, which is full of life and energy, turns into winter, which is still and seemingly without life. It reminds us of Nietzsche's Zarathustra and the winter doctrine: When the water is spanned by planks, when bridges and railings leap over the river, verily those are believed who say, “everything is in flux. . .” But when the winter comes . . . , then verily, not only the blockheads say, “Does not everything stand still?” “At bottom everything stands still.”—that is truly a winter doctrine (Bennett and Connolly 150). So we encounter the paradox of how to accommodate motion within stillness and stillness within motion: if everything is in continuous movement, how can there be stillness and regularity (and vice versa)? An interesting example of such temporal interruption is described by Giorgio Agamben who invokes an example with a tick that was kept alive, in a state of hibernation, for 18 years without nourishment (47). During those years this tick had ceased to exist in time, it existed only in extended space. There are of course differences between the tick and von Hagens’s plastinates—one difference being that the plastinates are not only dead but also plastic and inorganic—but the analogy points us to the idea of producing the conditions of possibility for eternal, timeless (and, by implication, motionless) bodies. If movement and change are thought of as spatial, as in Zeno’s paradox, here they have become temporal: movement happens in and because of time and not in space. The technique of plastination and the plastinates themselves emerge as processes of a-temporalisation and re-spatialisation of the body. The body is displaced—pulled out of time and history—and becomes a Cartesian body located entirely in the coordinates of extended space. As Ian Hacking suggests, plastinates are “Cartesian, extended, occupying space. Plastinated organs and corpses are odourless: like the Cartesian body, they can be seen but not smelt” (15).Interestingly, Body Worlds purports to show the inner workings of the human body. However, what visitors experience is not the working but the being. They do not see what the body does, its activities over time; rather, they see what it is, in space. Conversely, von Hagens wishes to “make us aware of our physical nature, our nature within us” (Kuppers 127), but the nature that we become aware of is not the messy, smelly and fluid nature of bodily interiors. Rather we encounter the still nature of Zarathustra’s winter landscape, a landscape in which the passage of time has come to a halt. As Walter concludes “the Body Worlds experience is primarily visual, spatial, static and odourless” (619).Still in Constant MotionAnd yet...Body Worlds moves us. If not for the fact that these plastinates and their creator strike us as gruesome, horrific and controversial, then because these bodies that we encounter touch us and we them. The sensation of movement, in and through the exhibition, is about this tension between being struck, touched or moved by a body that is radically foreign and yet strangely familiar to us. The resonant and reverberating movement that connects us with it is expressed through that (in)ability to accommodate motion in stillness, and stillness in motion. For whereas the plastinates are immobilised in space, they move in time and in experience. As Nigel Thrift puts it The body is in constant motion. Even at rest, the body is never still. As bodies move they trace out a path from one location to another. These paths constantly intersect with those of others in a complex web of biographies. These others are not just human bodies but also all other objects that can be described as trajectories in time-space: animals, machines, trees, dwellings, and so on (Thrift 8).This understanding of the body as being in constant motion stretches beyond the idea of a body that literally moves in physical space; it stresses the processual intertwining of subjects and objects through space-times that are enduring and evolving. The paradoxical nature of the relation between bodies in motion and bodies at rest is obviously far from exhausted through the brief exemplification that I have tried to provide here. Therefore I must end here and let someone else, better suited for this task, explain what it is that I wish to have said. We are hardly conscious of anything metaphorical when we say of one picture or of a story that it is dead, and of another that it has life. To explain just what we mean when we say this, is not easy. Yet the consciousness that one thing is limp, that another one has the heavy inertness of inanimate things, while another seems to move from within arises spontaneously. There must be something in the object that instigates it (Dewey 182). References Agamben, Giorgio. The Open. Trans. Kevin Attell. Stanford: Stanford U P, 2004.Bennett, Jane, and William Connolly. “Contesting Nature/Culture.” Journal of Nietzsche Studies 24 (2002) 148-163.Benthien, Claudia. Skin: On the Cultural Border Between Self and the World. Trans. Thomas Dunlap. New York: Columbia U P, 2002. California Science Center. “Summary of Ethical Review.” 10 Jan. 2009.Bergson, Henri. The Creative Mind. Trans. Mabelle Andison. Mineola: Dover, 2007. –––. Creative Evolution. Trans. Arthur Mitchell. New York: Cosimo Classics, 2005Deleuze, Gilles. Spinoza: Practical Philosophy. Trans. Robert Hurley. San Francisco: City Lights, 1988.Dewey, John. Art as Experience. New York: Perigee, 2005.Doyle, Richard. Wetwares. Minnesota: Minnesota U P, 2003.Hacking, Ian. “The Cartesian Body.” Biosocieties 1 (2006) 13-15.Hirschauer, Stefan. “Animated Corpses: Communicating with Post Mortals in an Anatomical Exhibition.” Body & Society 12.4 (2006) 25-52.Kuppers, Petra. “Visions of Anatomy: Exhibitions and Dense Bodies.” differences 15.3 (2004) 123-156.Manning, Erin. Politics of Touch: Sense, Movement, Sovereignty. Minnesota: Minnesota UP, 2007. Spindler, Konrad. The Man in the Ice. London: Weidenfeld and Nicolson, 1994.Thrift, Nigel. Spatial Formations. London: Sage, 1996.Von Hagens, Gunther, and Angelina Whalley. Body Worlds: The Original Exhibition of Real Human Bodies. Heidelberg: Institute for Plastination, 2008.Walter, Tony. “Plastination for Display: A New Way to Dispose of the Dead.” Journal of the Royal Anthropological Institute 10.3 (2004) 603-627.

At a time when a trip abroad was out of the reach of most women, even if they could not make the journey, Australian women could imagine “abroad” just by reading popular women’s magazines such as Woman (later Woman’s Day and Home then Woman’s Day) and The Australian Women’s Weekly, and journals, such as The Progressive Woman and The Housewife. Increasingly in the post-war period, these magazines and journals contained advertisements for holidaying abroad, recipes for international foods and articles on overseas fashions. It was not unusual for local manufacturers, to use the lure of travel and exotic places as a way of marketing their goods. Healing Bicycles, for example, used the slogan “In Venice men go to work on Gondolas: In Australia it’s a Healing” (“Healing Cycles” 40), and Exotiq cosmetics featured landscapes of countries where Exotiq products had “captured the hearts of women who treasured their loveliness: Cincinnati, Milan, New York, Paris, Geneva and Budapest” (“Exotiq Cosmetics” 36).Unlike Homer’s Penelope, who stayed at home for twenty years waiting for Odysseus to return from the Trojan wars, women have always been on the move to the same extent as men. Their rich travel stories (Riggal, Haysom, Lancaster)—mostly written as letters and diaries—remain largely unpublished and their experiences are not part of the public record to the same extent as the travel stories of men. Ros Pesman argues that the women traveller’s voice was one of privilege and authority full of excitement and disbelief (Pesman 26). She notes that until well into the second part of the twentieth century, “the journey for Australian women to Europe was much more than a return to the sources of family identity and history” (19). It was also:a pilgrimage to the centres and sites of culture, literature and history and an encounter with “the real world.”Europe, and particularly London,was also the place of authority and reference for all those seeking accreditation and recognition, whether as real writers, real ladies or real politicians and statesmen. (19)This article is about two Australian writers; Helen Seager, a journalist employed by The Argus, a daily newspaper in Melbourne Australia, and Gwen Hughes, a graduate of Emily McPherson College of Domestic Economy in Melbourne, working in England as a lecturer, demonstrator and cookbook writer for Parkinsons’ Stove Company. Helen Seager travelled to England on an assignment for The Argus in 1950 and sent articles each day for publication in the women’s section of the newspaper. Gwen Hughes travelled extensively in the Balkans in the 1930s recording her impressions, observations, and recipes for traditional foods whilst working for Parkinsons in England. These women were neither returning to the homeland for an encounter with the real world, nor were they there as cultural tourists in the Cook’s Tour sense of the word. They were professional writers and their observations about the places they visited offer fresh and lively versions of England and Europe, its people, places, and customs.Helen SeagerAustralian Journalist Helen Seager (1901–1981) wrote a daily column, Good Morning Ma’am in the women’s pages of The Argus, from 1947 until shortly after her return from abroad in 1950. Seager wrote human interest stories, often about people of note (Golding), but with a twist; a Baroness who finds knitting exciting (Seager, “Baroness” 9) and ballet dancers backstage (Seager, “Ballet” 10). Much-loved by her mainly female readership, in May 1950 The Argus sent her to England where she would file a daily report of her travels. Whilst now we take travel for granted, Seager was sent abroad with letters of introduction from The Argus, stating that she was travelling on a special editorial assignment which included: a certificate signed by the Lord Mayor of The City of Melbourne, seeking that any courtesies be extended on her trip to England, the Continent, and America; a recommendation from the Consul General of France in Australia; and introductions from the Premier’s Department, the Premier of Victoria, and Austria’s representative in Australia. All noted the nature of her trip, her status as an esteemed reporter for a Melbourne newspaper, and requested that any courtesy possible to be made to her.This assignment was an indication that The Argus valued its women readers. Her expenses, and those of her ten-year-old daughter Harriet, who accompanied her, were covered by the newspaper. Her popularity with her readership is apparent by the enthusiastic tone of the editorial article covering her departure. Accompanied with a photograph of Seager and Harriet boarding the aeroplane, her many women readers were treated to their first ever picture of what she looked like:THOUSANDS of "Argus" readers, particularly those in the country, have wanted to know what Helen Seager looks like. Here she is, waving good-bye as she left on the first stage of a trip to England yesterday. She will be writing her bright “Good Morning, Ma'am” feature as she travels—giving her commentary on life abroad. (The Argus, “Goodbye” 1)Figure 1. Helen Seager and her daughter Harriet board their flight for EnglandThe first article “From Helen in London” read,our Helen Seager, after busy days spent exploring England with her 10-year-old daughter, Harriet, today cabled her first “Good Morning, Ma’am” column from abroad. Each day from now on she will report from London her lively impressions in an old land, which is delightfully new to her. (Seager, “From Helen” 3)Whilst some of her dispatches contain the impressions of the awestruck traveller, for the most they are exquisitely observed stories of the everyday and the ordinary, often about the seemingly most trivial of things, and give a colourful, colonial and egalitarian impression of the places that she visits. A West End hair-do is described, “as I walked into that posh looking establishment, full of Louis XV, gold ornateness to be received with bows from the waist by numerous satellites, my first reaction was to turn and bolt” (Seager, “West End” 3).When she visits Oxford’s literary establishments, she is, for this particular article, the awestruck Australian:In Oxford, you go around saying, soto voce and aloud, “Oh, ye dreaming spires of Oxford.” And Matthew Arnold comes alive again as a close personal friend.In a weekend, Ma’am, I have seen more of Oxford than lots of native Oxonians. I have stood and brooded over the spit in Christ Church College’s underground kitchens on which the oxen for Henry the Eighth were roasted.I have seen the Merton Library, oldest in Oxford, in which the chains that imprisoned the books are still to be seen, and have added by shoe scrape to the stone steps worn down by 500 years of walkers. I have walked the old churches, and I have been lost in wonder at the goodly virtues of the dead. And then, those names of Oxford! Holywell, Tom’s Quad, Friars’ Entry, and Long Wall. The gargoyles at Magdalen and the stones untouched by bombs or war’s destruction. It adds a new importance to human beings to know that once, if only, they too have walked and stood and stared. (Seager, “From Helen” 3)Her sense of wonder whilst in Oxford is, however, moderated by the practicalities of travel incorporated into the article. She continues to describe the warnings she was given, before her departure, of foreign travel that had her alarmed about loss and theft, and the care she took to avoid both. “It would have made you laugh, Ma’am, could you have seen the antics to protect personal property in the countries in transit” (Seager, “From Helen” 3).Her description of a trip to Blenheim Palace shows her sense of fun. She does not attempt to describe the palace or its contents, “Blenheim Palace is too vast and too like a great Government building to arouse much envy,” settling instead on a curiosity should there be a turn of events, “as I surged through its great halls with a good-tempered, jostling mob I couldn’t help wondering what those tired pale-faced guides would do if the mob mood changed and it started on an old-fashioned ransack.” Blenheim palace did not impress her as much as did the Sunday crowd at the palace:The only thing I really took a fancy to were the Venetian cradle, which was used during the infancy of the present Duke and a fine Savvonerie carpet in the same room. What I never wanted to see again was the rubbed-fur collar of the lady in front.Sunday’s crowd was typically English, Good tempered, and full of Cockney wit, and, if you choose to take your pleasures in the mass, it is as good a company as any to be in. (Seager, “We Look” 3)In a description of Dublin and the Dubliners, Seager describes the food-laden shops: “Butchers’ shops leave little room for customers with their great meat carcasses hanging from every hook. … English visitors—and Dublin is awash with them—make an orgy of the cakes that ooze real cream, the pink and juicy hams, and the sweets that demand no points” (Seager, “English” 6). She reports on the humanity of Dublin and Dubliners, “Dublin has a charm that is deep-laid. It springs from the people themselves. Their courtesy is overlaid with a real interest in humanity. They walk and talk, these Dubliners, like Kings” (ibid.).In Paris she melds the ordinary with the noteworthy:I had always imagined that the outside of the Louvre was like and big art gallery. Now that I know it as a series of palaces with courtyards and gardens beyond description in the daytime, and last night, with its cleverly lighted fountains all aplay, its flags and coloured lights, I will never forget it.Just now, down in the street below, somebody is packing the boot of a car to go for, presumably, on a few days’ jaunt. There is one suitcase, maybe with clothes, and on the footpath 47 bottles of the most beautiful wines in the world. (Seager, “When” 3)She writes with a mix of awe and ordinary:My first glimpse of that exciting vista of the Arc de Triomphe in the distance, and the little bistros that I’ve always wanted to see, and all the delights of a new city, […] My first day in Paris, Ma’am, has not taken one whit from the glory that was London. (ibid.) Figure 2: Helen Seager in ParisIt is my belief that Helen Seager intended to do something with her writings abroad. The articles have been cut from The Argus and pasted onto sheets of paper. She has kept copies of the original reports filed whist she was away. The collection shows her insightful egalitarian eye and a sharp humour, a mix of awesome and commonplace.On Bastille Day in 1950, Seager wrote about the celebrations in Paris. Her article is one of exuberant enthusiasm. She writes joyfully about sirens screaming overhead, and people in the street, and looking from windows. Her article, published on 19 July, starts:Paris Ma’am is a magical city. I will never cease to be grateful that I arrived on a day when every thing went wrong, and watched it blossom before my eyes into a gayness that makes our Melbourne Cup gala seem funeral in comparison.Today is July 14.All places of business are closed for five days and only the places of amusement await the world.Parisians are tireless in their celebrations.I went to sleep to the music of bands, dancing feet and singing voices, with the raucous but cheerful toots from motors splitting the night air onto atoms. (Seager, “When” 3)This article resonates uneasiness. How easily could those scenes of celebration on Bastille Day in 1950 be changed into the scenes of carnage on Bastille Day 2016, the cheerful toots of the motors transformed into cries of fear, the sirens in the sky from aeroplanes overhead into the sirens of ambulances and police vehicles, as a Mohamed Lahouaiej Bouhlel, as part of a terror attack drives a truck through crowds of people celebrating in Nice.Gwen HughesGwen Hughes graduated from Emily Macpherson College of Domestic Economy with a Diploma of Domestic Science, before she travelled to England to take up employment as senior lecturer and demonstrator of Parkinson’s England, a company that manufactured electric and gas stoves. Hughes wrote in her unpublished manuscript, Balkan Fever, that it was her idea of making ordinary cooking demonstration lessons dramatic and homelike that landed her the job in England (Hughes, Balkan 25-26).Her cookbook, Perfect Cooking, was produced to encourage housewives to enjoy cooking with their Parkinson’s modern cookers with the new Adjusto temperature control. The message she had to convey for Parkinsons was: “Cooking is a matter of putting the right ingredients together and cooking them at the right temperature to achieve a given result” (Hughes, Perfect 3). In reality, Hughes used this cookbook as a vehicle to share her interest in and love of Continental food, especially food from the Balkans where she travelled extensively in the 1930s.Recipes of Continental foods published in Perfect Cooking sit seamlessly alongside traditional British foods. The section on soup, for example, contains recipes for Borscht, a very good soup cooked by the peasants of Russia; Minestrone, an everyday Italian soup; Escudella, from Spain; and Cream of Spinach Soup from France (Perfect 22-23). Hughes devoted a whole chapter to recipes and descriptions of Continental foods labelled “Fascinating Foods From Far Countries,” showing her love and fascination with food and travel. She started this chapter with the observation:There is nearly as much excitement and romance, and, perhaps fear, about sampling a “foreign dish” for the “home stayer” as there is in actually being there for the more adventurous “home leaver”. Let us have a little have a little cruise safe within the comfort of our British homes. Let us try and taste the good things each country is famed for, all the while picturing the romantic setting of these dishes. (Hughes, Perfect 255)Through her recipes and descriptive passages, Hughes took housewives in England and Australia into the strange and wonderful kitchens of exotic women: Madame Darinka Jocanovic in Belgrade, Miss Anicka Zmelova in Prague, Madame Mrskosova at Benesova. These women taught her to make wonderful-sounding foods such as Apfel Strudel, Knedlikcy, Vanilla Kipfel and Christmas Stars. “Who would not enjoy the famous ‘Goose with Dumplings,’” she declares, “in the company of these gay, brave, thoughtful people with their romantic history, their gorgeously appareled peasants set in their richly picturesque scenery” (Perfect 255).It is Hughes’ unpublished manuscript Balkan Fever, written in Melbourne in 1943, to which I now turn. It is part of the Latrobe Heritage collection at the State Library of Victoria. Her manuscript was based on her extensive travels in the Balkans in the 1930s whilst she lived and worked in England, and it was, I suspect, her intention to seek publication.In her twenties, Hughes describes how she set off to the Balkans after meeting a fellow member of the Associated Country Women of the World (ACWW) at the Royal Yugoslav Legation. He was an expert on village life in the Balkans and advised her, that as a writer she would get more information from the local villagers than she would as a tourist. Hughes, who, before television gave cooking demonstrations on the radio, wrote, “I had been writing down recipes and putting them in books for years and of course the things one talks about over the air have to be written down first—that seemed fair enough” (Hughes, Balkan 25-26). There is nothing of the awestruck traveller in Hughes’ richly detailed observations of the people and the places that she visited. “Travelling in the Balkans is a very different affair from travelling in tourist-conscious countries where you just leave it to Cooks. You must either have unlimited time at your disposal, know the language or else have introductions that will enable the right arrangements to be made for you” (Balkan 2), she wrote. She was the experiential tourist, deeply immersed in her surroundings and recording food culture and society as it was.Hughes acknowledged that she was always drawn away from the cities to seek the real life of the people. “It’s to the country district you must go to find the real flavour of a country and the heart of its people—especially in the Balkans where such a large percentage of the population is agricultural” (Balkan 59). Her descriptions in Balkan Fever are a blend of geography, history, culture, national songs, folklore, national costumes, food, embroidery, and vivid observation of the everyday city life. She made little mention of stately homes or buildings. Her attitude to travel can be summed up in her own words:there are so many things to see and learn in the countries of the old world that, walking with eyes and mind wide open can be an immensely delightful pastime, even with no companion and nowhere to go. An hour or two spent in some unpretentious coffee house can be worth all the dinners at Quaglino’s or at The Ritz, if your companion is a good talker, a specialist in your subject, or knows something of the politics and the inner life of the country you are in. (Balkan 28)Rather than touring the grand cities, she was seduced by the market places with their abundance of food, colour, and action. Describing Sarajevo she wrote:On market day the main square is a blaze of colour and movement, the buyers no less colourful than the peasants who have come in from the farms around with their produce—cream cheese, eggs, chickens, fruit and vegetables. Handmade carpets hung up for sale against walls or from trees add their barbaric colour to the splendor of the scene. (Balkan 75)Markets she visited come to life through her vivid descriptions:Oh those markets, with the gorgeous colours, and heaped untidiness of the fruits and vegetables—paprika, those red and green peppers! Every kind of melon, grape and tomato contributing to the riot of colour. Then there were the fascinating peasant embroideries, laces and rich parts of old costumes brought in from the villages for sale. The lovely gay old embroideries were just laid out on a narrow carpet spread along the pavement or hung from a tree if one happened to be there. (Balkan 11)Perhaps it was her radio cooking shows that gave her the ability to make her descriptions sensorial and pictorial:We tasted luxurious foods, fish, chickens, fruits, wines, and liqueurs. All products of the country. Perfect ambrosial nectar of the gods. I was entirely seduced by the rose petal syrup, fragrant and aromatic, a red drink made from the petals of the darkest red roses. (Balkan 151)Ordinary places and everyday events are beautifully realised:We visited the cheese factory amongst other things. … It was curious to see in that far away spot such a quantity of neatly arranged cheeses in the curing chamber, being prepared for export, and in another room the primitive looking round balls of creamed cheese suspended from rafters. Later we saw trains of pack horses going over the mountains, and these were probably the bearers of these cheeses to Bitolj or Skoplje, whence they would be consigned further for export. (Balkan 182)ConclusionReading Seager and Hughes, one cannot help but be swept along on their travels and take part in their journeys. What is clear, is that they were inspired by their work, which is reflected in the way they wrote about the places they visited. Both sought out people and places that were, as Hughes so vividly puts it, not part of the Cook’s Tour. They travelled with their eyes wide open for experiences that were both new and normal, making their writing relevant even today. Written in Paris on Bastille Day 1950, Seager’s Bastille Day article is poignant when compared to Bastille Day in France in 2016. Hughes’s descriptions of Sarajevo are a far cry from the scenes of destruction in that city between 1992 and 1995. The travel writing of these two women offers us vivid impressions and images of the often unreported events, places, daily lives, and industry of the ordinary and the then every day, and remind us that the more things change, the more they stay the same.Pesman writes, “women have always been on the move and Australian women have been as numerous as passengers on the outbound ships as have men” (20), but the records of their travels seldom appear on the public record. Whilst their work-related writings are part of the public record (see Haysom; Lancaster; Riggal), this body of women’s travel writing has not received the attention it deserves. Hughes’ cookbooks, with their traditional Eastern European recipes and evocative descriptions of people and kitchens, are only there for the researcher who knows that cookbooks are a trove of valuable social and cultural material. Digital copies of Seager’s writing can be accessed on Trove (a digital repository), but there is little else about her or her body of writing on the public record.ReferencesThe Argus. “Goodbye Ma’am.” 26 May 1950: 1. <http://trove.nla.gov.au/newspaper/article/22831285?searchTerm=Goodbye%20Ma%E2%80%99am%E2%80%99&searchLimits=l-title=13|||l-decade=195>.“Exotiq Cosmetics.” Advertisement. Woman 20 Aug. 1945: 36.Golding, Peter. “Just a Chattel of the Sale: A Mostly Light-Hearted Retrospective of a Diverse Life.” In Jim Usher, ed., The Argus: Life & Death of Newspaper. North Melbourne: Australian Scholarly Publishing 2007.Haysom, Ida. Diaries and Photographs of Ida Haysom. <http://search.slv.vic.gov.au/MAIN:Everything:SLV_VOYAGER1637361>.“Healing Cycles.” Advertisement. Woman 27 Aug. 1945: 40. Hughes, Gwen. Balkan Fever. Unpublished Manuscript. State Library of Victoria, MS 12985 Box 3846/4. 1943.———. Perfect Cooking London: Parkinsons, c1940.Lancaster, Rosemary. Je Suis Australienne: Remarkable Women in France 1880-1945. Crawley WA: UWA Press, 2008.Pesman, Ros. “Overseas Travel of Australian Women: Sources in the Australian Manuscripts Collection of the State Library of Victoria.” The Latrobe Journal 58 (Spring 1996): 19-26.Riggal, Louie. (Louise Blanche.) Diary of Italian Tour 1905 February 21 - May 1. <http://search.slv.vic.gov.au/MAIN:Everything:SLV_VOYAGER1635602>.Seager, Helen. “Ballet Dancers Backstage.” The Argus 10 Aug. 1944: 10. <http://trove.nla.gov.au/newspaper/article/11356057?searchTerm=Ballet%20Dancers%20Backstage&searchLimits=l-title=13|||l-decade=194>.———. “The Baroness Who Finds Knitting Exciting.” The Argus 1 Aug. 1944: 9. <http://trove.nla.gov.au/newspaper/article/11354557?searchTerm=Helen%20seager%20Baroness&searchLimits=l-title=13|||l-decade=194>.———. “English Visitors Have a Food Spree in Eire.” The Argus 29 Sep. 1950: 6. <http://trove.nla.gov.au/newspaper/article/22912011?searchTerm=English%20visitors%20have%20a%20spree%20in%20Eire&searchLimits=l-title=13|||l-decade=195>.———. “From Helen in London.” The Argus 20 June 1950: 3. <http://trove.nla.gov.au/newspaper/article/22836738?searchTerm=From%20Helen%20in%20London&searchLimits=l-title=13|||l-decade=195>.———. “Helen Seager Storms Paris—Paris Falls.” The Argus 15 July 1950: 7.<http://trove.nla.gov.au/newspaper/article/22906913?searchTerm=Helen%20Seager%20Storms%20Paris%E2%80%99&searchLimits=l-title=13|||l-decade=195>.———. “We Look over Blenheim Palace.” The Argus 28 Sep. 1950: 3. <http://trove.nla.gov.au/newspaper/article/22902040?searchTerm=Helen%20Seager%20Its%20as%20a%20good%20a%20place%20as%20you%20would%20want%20to%20be&searchLimits=l-title=13|||l-decade=195>.———. “West End Hair-Do Was Fun.” The Argus 3 July 1950: 3. <http://trove.nla.gov.au/newspaper/article/22913940?searchTerm=West%20End%20hair-do%20was%20fun%E2%80%99&searchLimits=l-title=13|||l-decade=195>.———. “When You Are in Paris on July 14.” The Argus 19 July 1950: 3. <http://trove.nla.gov.au/newspaper/article/22906244?searchTerm=When%20you%20are%20in%20Paris%20on%20July%2014&searchLimits=l-title=13|||l-decade=195>.

Jessica craned her head to take in the imposing, stone building, then lowered her gaze to the gold-plated sign at the base of the steps. “Institute of Methodology”, it read. Inside the heavy iron doors, a woman sat at a desk, her face devoid of expression. “Subject area?” asked the woman. “Uhmm, feminism ... and fiction, I think.” “Turn right.” “Do you have a map?” “No.” “How am I meant to find things?” “Each has their own method; it’s not up to us to prescribe that.” Jessica sighed, readjusted her handbag and turned right. A corridor stretched out before her. She set off, her stiletto boots echoing on the hard wooden floor. The first door she arrived at had the words “Deleuze and Guattari” positioned squarely in the middle. She hesitated, then turned the doorknob. The room was white and empty. A male voice issued from somewhere but she couldn’t tell the direction from which it came. It droned on, with some inflection, but there was no way of knowing where the sentences started and finished. She picked out a few words: a thousand plateaus, becoming, burrowing, but couldn’t piece them into anything meaningful. She backed out of the room, frowning, and asked me, How am I going to learn anything if they only have these voices? I can’t lipread them. And how can I produce something factual if I haven’t heard it all? I might make stuff up. You always make things up anyway. After the barrier of disembodied sound, the silence of the corridor was soothing. Jessica always had difficulty with hearing men’s voices, for their registers were lower. Sometimes, she wondered if this was the reason she’d become interested in feminism: women were simply easier to understand. The next door was labelled “Facets of Phenomenology.” After that was “Post-It Notes and Poststructuralism”, “Interpretation of Geometric Design”, “Knitting Class” and “Cyberspace and Geography.” None of these were very helpful. She wanted something on bodies and writing. She walked on. It was, she soon realised, so terribly easy to lose one’s way. The corridors continued. She turned right most of the time, and occasionally left. Her arches began to ache. After a while she came to the conclusion that she had no idea of where she was. Immediately, a bird appeared and dived down her throat. Trapped, it thudded against her ribs. Breathe, I told her. Breathe. She put a hand out to the wall. Outside another door she heard, a voice with a distinct Australian accent. She checked the label on the door. “Fictocriticism,” it read. The door opened. The bird climbed out of her chest and flew away. A young woman stood before her, wearing bright red lipstick. “We saw your shadow beneath the door.” She pointed to Jessica’s feet. “We don’t like barriers, so come in.” The room was airy and brilliantly lit, with a high ceiling patterned with pressed metal vines and flowers. A man and a handful of women sat at a table covered with papers, bottles of wine, brie, sundried tomatoes and crackers. “Wine?” asked the woman, a bottle in her hand. “It’s from Margaret River.” “Oh yes, please.” Jessica pulled out a chair from the table. The people’s faces looked friendly. “What brings you here?” The woman with red lipstick asked, handing her a glass. “I’m trying to find a writing style that’s comfortable for me to use. I just can’t relate to abstract texts, like those by Deleuze and Guattari.” Jessica eyed the cheese platter on the table. She was hungry. “Help yourself,” said the man. Jessica picked up the cheese knife and a cracker. “You’d like my essay, then, ‘Me and My Shadow.’” It was an older woman speaking, with soft grey hair and luminous eyes. “In it I assert that Guattari’s Molecular Revolution is distancing and, she pushed the pile of paper napkins towards Jessica, ‘totally abstract and impersonal. Though the author uses the first person (‘The distinction I am proposing’, ‘I want therefore to make it clear’), it quickly became clear to me that he had no interest whatsoever in the personal, or in concrete situations as I understand them – a specific person, a specific machine, somewhere in time and space, with something on his/her mind, real noises, smells, aches and pains” (131). Jessica thought about the first room, where Deleuze’s and Guattari’s voices had seemed to issue from nowhere. “Of course,” she said. “If my comprehension comes from reading faces and bodies, it follows that those writers who evince themselves in the text will be the ones that appeal to me.” The rest of the table was silent. “I’m deaf,” Jessica explained. “I’ve no hearing in my left ear and half in my right, but people don’t know until I tell them.” “I’d never have guessed,” said the woman with red lipstick. “I’m good at faking it,” Jessica replied wryly. “It seems to me that, if I only hear some things and make the rest up, then my writing should reflect that.” “We might be able to help you — we write about, and in the style of, fictocriticism.” Two women were talking at once. It was difficult to tell who was saying what. “But what is it?” Jessica asked. “That’s a problematic question. It resists definition, you see, for the form it takes varies according to the writer.” She glanced from one woman to the other. It was hard to keep up. They went on, “Fictocriticism might most usefully be defined as hybridised writing that moves between the poles of fiction (‘invention’/‘speculation’) and criticism (‘deduction’/‘explication’), of subjectivity (‘interiority’) and objectivity (‘exteriority’). It is writing that brings the ‘creative’ and the ‘critical’ together – not simply in the sense of placing them side by side, but in the sense of mutating both, of bringing a spotlight to bear upon the known forms in order to make them ‘say’ something else” (Kerr and Nettlebeck 3). “It began to incorporate narratives and styles that wrote against omniscience in favour of fragmentary, personal perspectives.” Concentrating on cutting and spreading her brie, Jessica couldn’t see who had said this. She looked up, trying to see who had spoken. “In addition,” said a young, slim woman, “The use of autobiographical elements in ficto-criticism that include the body and personal details … realises a subjectivity that is quite different from the controlling academic critical subject with their voice from on high” (Flavell 77). Jessica bit into her cracker. The brie was creamy, but rather too strong. She piled sundried tomatoes onto it. “It is of course, a capacious category,” the man added, “as it must be if it is inspired by the materials and situation at hand. One might urge the interested writer not to feel that their practice has to conform to one or another model, but to have the confidence that the problem characterising the situation before them will surprise them into changing their practices. Like all literature, fictocriticism experiments with ways of being in the world, with forms of subjectivity if you like” (Muecke 15). Jessica nodded, her mouth full of biscuit and brie. Oil dripped from the tomatoes down her fingers. “Yes,” it was the two women in their duet, “in fictocritical writings the ‘distance’ of the theorist/critic collides with the ‘interiority’ of the author. In other words, the identity of the author is very much at issue. This is not to say that an ‘identity’ declares itself strictly in terms of the lived experience of the individual, but it does declare itself as a politic to be viewed, reviewed, contested, and above all engaged with” (Kerr and Nettlebeck 3). “That makes sense,” Jessica thought aloud. “Everything I write is an amalgam of fact and fiction, because I hear some things and make the rest up. Deafness influences the way I process and write about the world, so it seems I can’t avoid my body when I write.” She lifted a napkin from the pile and wiped her oily fingers. “Yet, to use a language of the body, or écriture féminine, is also to run the risk of essentialism, of assuming that, for example when we write long, silky sentences, we are saying that this is how every woman would write. It’s also true that, when writing, we don’t have to be limited to our own bodies – we can go beyond them.” She paused, thinking. “It’s been said that sign language is a form of écriture féminine, for a person who signs literally writes with their hands. Where are my notes?” She ferreted through her handbag, pushing aside tubes of lip gloss and hand cream, a bus pass and mirror, and extracted some folded pieces of paper. “Here, H-Dirksen L. Bauman comments on the possibilites of écriture féminine for the disabled, writing that, The project of recognizing Deaf identity bears similarities to the feminist project of re-gaining a ‘body of one’s own’ through linguistic and literary practices. Sign, in a more graphic way, perhaps, than l’écriture féminine is a ‘writing of/on the body.’ The relation between Sign and l’écriture féminine raises questions that could have interesting implications for feminist performance. Does the antiphonocetric nature of Sign offer a means of averting these essentializing tendency of l’écriture féminine? Does the four-dimensional space of performance offer ways of deconstructing phallogocentric linear discourse? (359) “As Sign is a writing by the body, it could be argued that each body produces an original language. I think it’s this, rather than antiphonocentrism — that is, refusing to privilege speech over writing, as has been the tradition — that represents the destabilising effects of Sign.” “Here’s Jamming the Machinery.” The slim woman pushed a book towards Jessica. “It’s about contemporary Australian écriture féminine.” Jessica opened the covers and began reading: As a counter-strategy, écriture féminine, it is argued, is theoretically sourced in the bodies of women. Here, the body represents one aspect of what it ‘means’ to be a woman, but of course our bodies are infinitely variable as are our socio-historical relations and the way that we live through and make meaning of our particular bodies. Texts, however, are produced through the lived practices of being socially positioned as (among other things) women, so those effects will be inscribed in actively inventing ways for women to speak and write about ourselves as women, rather than through the narrative machinery of patriarchy (Bartlett 1-2). I agree with that, Jessica mused to herself. Even if, on paper, écriture féminine does run the risk of essentialism, it’s still a useful strategy, so long as one remains attentive to the specificity of each individual body. She looked up. The conversation was becoming loud, joyful and boisterous. It was turning into a party. Sadly, she stood. “I’d like to stay, but I have to keep thinking.” She pushed in her chair. “Thank you for your ideas.” “Goodbye and good luck!” they chorused, and replenished their wine glasses. Outside, it was getting dark. She trailed her fingers along the wall for balance. Her sight orientated her; without it, she was liable to fall over, particularly in stilettos. Seeing a movement near the ceiling, Jessica stopped and peered upwards. Dragons! she cried. Sitting in the rafters were three small, pearly white dragons, their scaly hides gleaming in the darkness. Here, she called, stretching out a hand. One dropped, swooping, and landed on her wrist, its talons gripping her arm. Ouch! It looked at her curiously with its small gold eyes, then stretched its wings proudly. Dark blue veins ran across the soft membrane. You’re not very cuddly, she told it, but you are exquisite. Tell me, are you real? For an answer, it leaned over and gently nipped her thumb, drawing blood. Its tail swished like a cat’s in a frisky mood. Stop making things up, I scolded her. This is supposed to be serious. Abruptly, the dragon sprang from her wrist, winging gracefully back to the ceiling. Jessica rubbed her arm and continued, feeling ripples of unevenly applied paint beneath her fingertips. Let me pose a question, I suggested: if a fairy godmother offered you your hearing, would you take it? Well, deafness has made me who I am— You mean, an opinionated, obnoxious, feminist thinker and writer? Yes, exactly. So perhaps I wouldn’t take it. And where would you be without silence, which has given you the space in which to think, and which has shaped you as a writer? Without silence, you wouldn’t have turned to words. Hmmm, yes. She slowed. It’s awfully dark in here now. And quiet. For deaf people, silence has often been yoked together with negative connotations – it’s a cave, a prison, a tomb. Sometimes it can feel like this, but, as you know, at other times it’s liberating. You don’t have to listen to someone yakking on their mobile phone on the bus, nor overhear your flatmate having loud sex in the room above; you can simply switch off your hearing aid and keep reading your book, or thinking your thoughts. In a somewhat similar situation, Stephen Hawking, the theoretical physicist, has said that ‘his disability has given him the advantage of having more time to think,’ although Susan Wendell points out that he is only able to do this ‘because of the help of his family, three nurses, a graduate student who travels with him to maintain his computer-communications systems’ – resources which are unavailable to many disabled people” (109). Thus although disability has been largely theorised as lack, it would seem that the contrary is the case: disability brings with it a wealth of possibility. Jessica slowed, feeling vibrations in the wall and beneath our feet. Her heartbeat quickened. Maybe it’s music. It’s not. It’s irregular. Then we heard the sound, like distant thunder. Get back against the wall, I ordered her. Seconds later a crowd of creatures ran past, rattling the floorboards. They were so black we couldn’t see them. What was that? she asked. They smelled like horses. Musky, but sharp too. Let’s get moving. And I told you to stop making things up. I didn’t make that up! she protested. Her pulse was still rapid, so I kept talking to distract her. The difficulty is to avoid referring to the disabled person as having lost something. Of course, you can lose your hearing, but you gain infinitely more in other ways – your senses of touch, taste, smell and sight are augmented. In the current climate of thinking, this is easier said than done. Lennard Davis indicates with distaste that discussions of disability stop theorists in their tracks. Disability, as it has been formulated, is a construct that is defined by lack. Rather than face this ragged imaged [of the disabled individual], the critic turns to the fluids of sexuality, the gloss of lubrication, the glossary of the body as text, the heteroglossia of the intertext, the glossolalia of the schizophrenic. But almost never the body of the differently abled (5). Theorists of disability consistently point out that, if more effort and energy were directed towards the philosophical implications of the disabled body, a wealth of new material and ideas would emerge that would shatter existing presumptions about the corporeal. For example, there are still immense possibilities thrown up by theorising a jouissance, or pleasure, in the disabled body. As Susan Wendell points out, “paraplegics and quadriplegics have revolutionary things to teach us about the possibilities of sexuality which contradict patriarchal culture’s obsessions with the genitals” (120). Thus if there were more of a focus on the positive aspects of disability and on promoting the understanding that disability is not about lack, people could see how it fosters creativity and imagination. Jessica saw with relief that there was a large bay window at the end of a corridor, looking out onto the Institute’s grounds. She collapsed onto the bench beneath it, which was layered with cushions. The last of the sun was fading and the grass refracted a golden sheen. She unzipped her boots and swung her legs onto the bench. Leaning her head back against the wall, she remembered a day at primary school when she was eleven. She sat on the blue seat beneath the Jacaranda tree, a book open in her lap. It was lunchtime, the sun was warm and purple Jacaranda blossoms lay scattered at her feet, some squidged wetly into the cement. She looked up from the book to watch her classmates playing soccer on the field, shouting and calling. She would have joined them, except that of late she had felt awkwardness, where before she had been blithe. She, who was so used to scrambling over the delightful hardness of wool bales in the shearing shed, who ran up and down the banks of creeks and crawled into ti trees, flakes of bark sticking to her jumper, had gradually, insidiously, learnt a consciousness of her body. She was not like them. We were silent. The electric lights on the walls of the building came on, illuminating sections of the stonework. At the time, she hated being isolated, but it forced to look at the world differently. Spending so much time on her own also taught her to listen to me, her imagination, and because of that her writing flourished. There was a flutter in the hallway. The tiny dragon had returned. It braked in the air, circled, and floated gently onto her skirt. Was this your doing? She asked me suspiciously. Maybe. She held out her palm. The dragon jumped into it, squeaking, its tail whipping lazily. Jessica smiled. References Bartlett, Alison. Jamming the Machinery: Contemporary Australian Women’s Writing. Toowoomba: Association for the Study of Australian Literature, 1998. Bauman, H-Dirksen L. “Toward a Poetics of Vision, Space and the Body.” The Disability Studies Reader. Ed. Lennard J. Davis. Hoboken: Routledge, 2006. 355-366. Davis, Lennard J. Enforcing Normalcy: Disability, Deafness, and the Body. London: Verso, 1995. Flavell, Helen. Writing-Between: Australian and Canadian Ficto-Criticism. Ph.D. Thesis. Murdoch University, 2004. Gibbs, Anna. “Writing and the Flesh of Others.” Australian Feminist Studies 18 (2003): 309–319. Kerr, Heather, and Amanda Nettlebeck. “Notes Towards an Introduction.” The Space Between: Australian Women Writing Fictocriticism. Ed. Heather Kerr and Amanda Nettlebeck. Nedlands: U of Western Australia P, 1998. 1-18. Muecke, Stephen. Joe in the Andamans: And Other Fictocritical Stories. Erskineville: Local Consumption Publications, 2008. Tompkins, Jane. “Me and My Shadow.” Gender and Theory: Dialogues on Feminist Criticism. Ed. Linda Kauffman. Oxford: Basil Blackwell, 1989. 121-139. Wendell, Susan. “Towards a Feminist Theory of Disability.” Hypatia 4 (1989): 104–124.

As the name suggests, the humanities is largely a study of the human condition, in which history sits as a discipline concerned with the past. Environmental history is a new field that brings together scholars from a range of disciplines to consider the changing relationships between humans and the environment over time. Critiques of anthropocentrism that place humans at the centre of the universe or make assessments through an exclusive human perspective provide a challenge to scholars to rethink our traditional biases against the nonhuman world. The movement towards nonhumanism or posthumanism, however, does not seem to have had much of an impression on history as a discipline. What would a nonhumanist history look like if we re-centred the historical narrative around pigs? There are histories of pigs as food (see for example, The Cambridge History of Food which has a chapter on “Hogs”). There are food histories that feature pork in terms of its relationship to multiethnic identity (such as Donna Gabaccia’s We Are What We Eat) and examples made of pigs to promote ethical eating (Singer). Pigs are central to arguments about dietary rules and what motivates them (Soler; Dolander). Ancient pig DNA has also been employed in studies on human migration and colonisation (Larson et al.; Durham University). Pigs are also widely used in a range of products that would surprise many of us. In 2008, Christien Meindertsma spent three years researching the products made from a single pig. Among some of the more unexpected results were: ammunition, medicine, photographic paper, heart valves, brakes, chewing gum, porcelain, cosmetics, cigarettes, hair conditioner and even bio diesel. Likewise, Fergus Henderson, who coined the term ‘nose to tail eating’, uses a pig on the front cover of the book of that name to suggest the extraordinary and numerous potential of pigs’ bodies. However, my intention here is not to pursue a discussion of how parts of their bodies are used, rather to consider a reorientation of the historical narrative to place pigs at the centre of stories of our co-evolution, in order to see what their history might say about humans and our relationships with them. This is underpinned by recognition of the inter-relationality of humans and animals. The relationships between wild boar and pigs with humans has been long and diverse. In a book exploring 10,000 years of interaction, Anton Ervynck and Peter Rowley-Conwy argue that pigs have been central to complex cultural developments in human societies and they played an important role in human migration patterns. The book is firmly grounded within the disciplines of zoology, anthropology and archaeology and contributes to an understanding of the complex and changing relationship humans have historically shared with wild boar and domestic pigs. Naturalist Lyall Watson also explores human/pig relationships in The Whole Hog. The insights these approaches offer for the discipline of history are valuable (although overlooked) but, more importantly, such scholarship also challenges a humanist perspective that credits humans exclusively with historical change and suggests, moreover, that we did it alone. Pigs occupy a special place in this history because of their likeness to humans, revealed in their use in transplant technology, as well as because of the iconic and paradoxical status they occupy in our lives. As Ervynck and Rowley-Conwy explain, “On the one hand, they are praised for their fecundity, their intelligence, and their ability to eat almost anything, but on the other hand, they are unfairly derided for their apparent slovenliness, unclean ways, and gluttonous behaviour” (1). Scientist Niamh O’Connell was struck by the human parallels in the complex social structures which rule the lives of pigs and people when she began a research project on pig behaviour at the Agricultural Research Institute at Hillsborough in County Down (Cassidy). According to O’Connell, pigs adopt different philosophies and lifestyle strategies to get the most out of their life. “What is interesting from a human perspective is that low-ranking animals tend to adopt one of two strategies,” she says. “You have got the animals who accept their station in life and then you have got the other ones that are continually trying to climb, and as a consequence, their life is very stressed” (qtd. in Cassidy). The closeness of pigs to humans is the justification for their use in numerous experiments. In the so-called ‘pig test’, code named ‘Priscilla’, for instance, over 700 pigs dressed in military uniforms were used to study the effects of nuclear testing at the Nevada (USA) test site in the 1950s. In When Species Meet, Donna Haraway draws attention to the ambiguities and contradictions promoted by the divide between animals and humans, and between nature and culture. There is an ethical and critical dimension to this critique of human exceptionalism—the view that “humanity alone is not [connected to the] spatial and temporal web of interspecies dependencies” (11). There is also that danger that any examination of our interdependencies may just satisfy a humanist preoccupation with self-reflection and self-reproduction. Given that pigs cannot speak, will they just become the raw material to reproduce the world in human’s own image? As Haraway explains: “Productionism is about man the tool-maker and -user, whose highest technical production is himself […] Blinded by the sun, in thrall to the father, reproduced in the sacred image of the same, his rewards is that he is self-born, an auto telic copy. That is the mythos of enlightenment and transcendence” (67). Jared Diamond acknowledges the mutualistic relationship between pigs and humans in Guns, Germs and Steel and the complex co-evolutionary path between humans and domesticated animals but his account is human-centric. Human’s relationships with pigs helped to shape human history and power relations and they spread across the world with human expansion. But questioning their utility as food and their enslavement to this cause was not part of the account. Pigs have no voice in the histories we write of them and so they can appear as passive objects in their own pasts. Traces of their pasts are available in humanity’s use of them in, for example, the sties built for them and the cooking implements used to prepare meals from them. Relics include bones and viruses, DNA sequences and land use patterns. Historians are used to dealing with subjects that cannot speak back, but they have usually left ample evidence of what they have said. In the process of writing, historians attempt to perform the miracle, as Curthoys and Docker have suggested, of restoration; bringing the people and places that existed in the past back to life (7). Writing about pigs should also attempt to bring the animal to life, to understand not just their past but also our own culture. In putting forward the idea of an alternative history that starts with pigs, I am aware of both the limits to such a proposal, and that most people’s only contact with pigs is through the meat they buy at the supermarket. Calls for a ban on intensive pig farming (RSPCA, ABC, AACT) might indeed have shocked people who imagine their dinner comes from the type of family farm featured in the movie Babe. Baby pigs in factory farms would have been killed a long time before the film’s sheep dog show (usually at 3 to 4 months of age). In fact, because baby pigs do grow so fast, 48 different pigs were used to film the role of the central character in Babe. While Babe himself may not have been aware of the relationship pigs generally have to humans, the other animals were very cognisant of their function. People eat pigs, even if they change the name of the form it takes in order to do so:Cat: You know, I probably shouldn’t say this, but I’m not sure if you realize how much the other animals are laughing at you for this sheep dog business. Babe: Why would they do that? Cat: Well, they say that you’ve forgotten that you’re a pig. Isn't that silly? Babe: What do you mean? Cat: You know, why pigs are here. Babe: Why are any of us here? Cat: Well, the cow’s here to be milked, the dogs are here to help the Boss's husband with the sheep, and I’m here to be beautiful and affectionate to the boss. Babe: Yes? Cat: [sighs softly] The fact is that pigs don’t have a purpose, just like ducks don’t have a purpose. Babe: [confused] Uh, I—I don’t, uh ... Cat: Alright, for your own sake, I’ll be blunt. Why do the Bosses keep ducks? To eat them. So why do the Bosses keep a pig? The fact is that animals don’t seem to have a purpose really do have a purpose. The Bosses have to eat. It’s probably the most noble purpose of all, when you come to think about it. Babe: They eat pigs? Cat: Pork, they call it—or bacon. They only call them pigs when they’re alive (Noonan). Babe’s transformation into a working pig to round up the sheep makes him more useful. Ferdinand the duck tried to do the same thing by crowing but was replaced by an alarm clock. This is a common theme in children’s stories, recalling Charlotte’s campaign to praise Wilbur the pig in order to persuade the farmer to let him live in E. B. White’s much loved children’s novel, Charlotte’s Web. Wilbur is “some pig”, “terrific”, “radiant” and “humble”. In 1948, four years before Charlotte’s Web, White had published an essay “Death of a Pig”, in which he fails to save a sick pig that he had bought in order to fatten up and butcher. Babe tried to present an alternative reality from a pig’s perspective, but the little pig was only spared because he was more useful alive than dead. We could all ask the question why are any of us here, but humans do not have to contemplate being eaten to justify their existence. The reputation pigs have for being filthy animals encourages distaste. In another movie, Pulp Fiction, Vincent opts for flavour, but Jules’ denial of pig’s personalities condemns them to insignificance:Vincent: Want some bacon? Jules: No man, I don’t eat pork. Vincent: Are you Jewish? Jules: Nah, I ain’t Jewish, I just don’t dig on swine, that’s all. Vincent: Why not? Jules: Pigs are filthy animals. I don’t eat filthy animals. Vincent: Bacon tastes gooood. Pork chops taste gooood. Jules: Hey, sewer rat may taste like pumpkin pie, but I’d never know ’cause I wouldn’t eat the filthy motherfucker. Pigs sleep and root in shit. That’s a filthy animal. I ain’t eat nothin’ that ain’t got sense enough to disregard its own feces [sic]. Vincent: How about a dog? Dogs eats its own feces. Jules: I don’t eat dog either. Vincent: Yeah, but do you consider a dog to be a filthy animal? Jules: I wouldn’t go so far as to call a dog filthy but they’re definitely dirty. But, a dog’s got personality. Personality goes a long way. Vincent: Ah, so by that rationale, if a pig had a better personality, he would cease to be a filthy animal. Is that true? Jules: Well we’d have to be talkin’ about one charming motherfuckin’ pig. I mean he’d have to be ten times more charmin’ than that Arnold on Green Acres, you know what I’m sayin’? In the 1960s television show Green Acres, Arnold was an exceptional pig who was allowed to do whatever he wanted. He was talented enough to write his own name and play the piano and his attempts at painting earned him the nickname “Porky Picasso”. These talents reflected values that are appreciated, and so he was. The term “pig” is, however, chiefly used a term of abuse, however, embodying traits we abhor—gluttony, obstinence, squealing, foraging, rooting, wallowing. Making a pig of yourself is rarely honoured. Making a pig of the humanities, however, could be a different story. As a historian I love to forage, although I use white gloves rather than a snout. I have rubbed my face and body on tree trunks in the service of forestry history and when the temperature rises I also enjoy wallowing, rolling from side to side rather than drawing a conclusion. More than this, however, pigs provide a valid means of understanding key historical transitions that define modern society. Significant themes in modern history—production, religion, the body, science, power, the national state, colonialism, gender, consumption, migration, memory—can all be understood through a history of our relationships with pigs. Pigs play an important role in everyday life, but their relationship to the economic, social, political and cultural matters discussed in general history texts—industrialisation, the growth of nation states, colonialism, feminism and so on—are generally ignored. However “natural” this place of pigs may seem, culture and tradition profoundly shape their history and their own contribution to those forces has been largely absent in history. What, then, would the contours of such a history that considered the intermeshing of humans and pigs look like? The intermeshing of pigs in early human history Agricultural economies based on domestic animals began independently in different parts of the world, facilitating increases in population and migration. Evidence for long-term genetic continuity between modern and ancient Chinese domestic pigs has been established by DNA sequences. Larson et al. have made an argument for five additional independent domestications of indigenous wild boar populations: in India, South East Asia and Taiwan, which they use to develop a picture of both pig evolution and the development and spread of early farmers in the Far East. Domestication itself involves transformation into something useful to animals. In the process, humans became transformed. The importance of the Fertile Crescent in human history has been well established. The area is attributed as the site for a series of developments that have defined human history—urbanisation, writing, empires, and civilisation. Those developments have been supported by innovations in food production and animal husbandry. Pig, goats, sheep and cows were all domesticated very early in the Fertile Crescent and remain four of the world’s most important domesticated mammals (Diamond 141). Another study of ancient pig DNA has concluded that the earliest domesticated pigs in Europe, believed to be descended from European wild boar, were introduced from the Middle East. The research, by archaeologists at Durham University, sheds new light on the colonisation of Europe by early farmers, who brought their animals with them. Keith Dobney explains:Many archaeologists believe that farming spread through the diffusion of ideas and cultural exchange, not with the direct migration of people. However, the discovery and analysis of ancient Middle Eastern pig remains across Europe reveals that although cultural exchange did happen, Europe was definitely colonised by Middle Eastern farmers. A combination of rising population and possible climate change in the ‘fertile crescent’, which put pressure on land and resources, made them look for new places to settle, plant their crops and breed their animals and so they rapidly spread west into Europe (ctd in ScienceDaily). Middle Eastern farmers colonised Europe with pigs and in the process transformed human history. Identity as a porcine theme Religious restrictions on the consumption of pigs come from the same area. Such restrictions exist in Jewish dietary laws (Kashrut) and in Muslim dietary laws (Halal). The basis of dietary laws has been the subject of much scholarship (Soler). Economic and health and hygiene factors have been used to explain the development of dietary laws historically. The significance of dietary laws, however, and the importance attached to them can be related to other purposes in defining and expressing religious and cultural identity. Dietary laws and their observance may have been an important factor in sustaining Jewish identity despite the dispersal of Jews in foreign lands since biblical times. In those situations, where a person eats in the home of someone who does not keep kosher, the lack of knowledge about your host’s ingredients and the food preparation techniques make it very difficult to keep kosher. Dietary laws require a certain amount of discipline and self-control, and the ability to make distinctions between right and wrong, good and evil, pure and defiled, the sacred and the profane, in everyday life, thus elevating eating into a religious act. Alternatively, people who eat anything are often subject to moral judgments that may also lead to social stigmatisation and discrimination. One of the most powerful and persuasive discourses influencing current thinking about health and bodies is the construction of an ‘obesity epidemic’, critiqued by a range of authors (see for example, Wright & Harwood). As omnivores who appear indiscriminate when it comes to food, pigs provide an image of uncontrolled eating, made visible by the body as a “virtual confessor”, to use Elizabeth Grosz’s term. In Fat Pig, a production by the Sydney Theatre Company in 2006, women are reduced to being either fat pigs or shrieking shallow women. Fatuosity, a blog by PhD student Jackie Wykes drawing on her research on fat and sexual subjectivity, provides a review of the play to describe the misogyny involved: “It leaves no options for women—you can either be a lovely person but a fat pig who will end up alone; or you can be a shrill bitch but beautiful, and end up with an equally obnoxious and shallow male counterpart”. The elision of the divide between women and pigs enacted by such imagery also creates openings for new modes of analysis and new practices of intervention that further challenge humanist histories. Such interventions need to make visible other power relations embedded in assumptions about identity politics. Following the lead of feminists and postcolonial theorists who have challenged the binary oppositions central to western ideology and hierarchical power relations, critical animal theorists have also called into question the essentialist and dualist assumptions underpinning our views of animals (Best). A pig history of the humanities might restore the central role that pigs have played in human history and evolution, beyond their exploitation as food. Humans have constructed their story of the nature of pigs to suit themselves in terms that are specieist, racist, patriarchal and colonialist, and failed to grasp the connections between the oppression of humans and other animals. The past and the ways it is constructed through history reflect and shape contemporary conditions. In this sense, the past has a powerful impact on the present, and the way this is re-told, therefore, also needs to be situated, historicised and problematicised. The examination of history and society from the standpoint of (nonhuman) animals offers new insights on our relationships in the past, but it might also provide an alternative history that restores their agency and contributes to a different kind of future. As the editor of Critical Animals Studies, Steve Best describes it: “This approach, as I define it, considers the interaction between human and nonhuman animals—past, present, and future—and the need for profound changes in the way humans define themselves and relate to other sentient species and to the natural world as a whole.” References ABC. “Changes to Pig Farming Proposed.” ABC News Online 22 May 2010. 10 Aug. 2010 http://www.abc.net.au/news/stories/2010/05/22/2906519.htm Against Animal Cruelty Tasmania. “Australia’s Intensive Pig Industry: The Intensive Pig Industry in Australia Has Much to Hide.” 10 Sep. 2010 http://www.aact.org.au/pig_industry.htm Babe. Dir. Chris Noonan. Universal Pictures, 1995. Best, Steven. “The Rise of Critical Animal Studies: Putting Theory into Action and Animal Liberation into Higher Education.” Journal for Critical Animal Studies 7.1 (2009): 9-53. Cassidy, Martin. “How Close are Pushy Pigs to Humans?”. BBC News Online 2005. 10 Sep. 2010 http://news.bbc.co.uk/2/hi/uk_news/northern_ireland/4482674.stmCurthoys, A., and Docker, J. “Time Eternity, Truth, and Death: History as Allegory.” Humanities Research 1 (1999) 10 Sep. 2010 http://www.anu.edu.au/hrc/publications/hr/hr_1_1999.phpDiamond, Jared. Guns, Germs and Steel: The Fates of Human Societies. New York: W. W. Norton, 1999. Dolader, Miguel-Àngel Motis. “Mediterranean Jewish Diet and Traditions in the Middle Ages”. Food: A Culinary History. Eds. Jean-Louis Flandrin and Massimo Montanari. Trans. Clarissa Botsford, Arthus Golhammer, Charles Lambert, Frances M. López-Morillas and Sylvia Stevens. New York: Columbia UP, 1999. 224-44. Durham University. “Chinese Pigs ‘Direct Descendants’ of First Domesticated Breeds.” ScienceDaily 20 Apr. 2010. 29 Aug. 2010 http://www.sciencedaily.com/releases/2010/04/100419150947.htm Gabaccia, Donna R. We Are What We Eat: Ethnic Food and the Making of Americans. Cambridge: Harvard University Press, 1998. Grosz, Elizabeth. Volatile Bodies: Toward a Corporeal Feminism. St Leonards: Allen & Unwin, 1994. Haraway, D. “The Promises of Monsters: A Regenerative Politics for Inappropriate/d Others.” The Haraway Reader. New York: Routledge, 2005. 63-124. Haraway, D. When Species Meet: Posthumanities. 3rd ed. London: University of Minnesota Press, 2008. Henderson, Fergus. Nose to Tail Eating: A Kind of British Cooking. London: Bloomsbury, 2004. Kiple, Kenneth F., Kriemhild Coneè Ornelas. Cambridge History of Food. Cambridge: Cambridge University Press, 2000. Larson, G., Ranran Liu, Xingbo Zhao, Jing Yuan, Dorian Fuller, Loukas Barton, Keith Dobney, Qipeng Fan, Zhiliang Gu, Xiao-Hui Liu, Yunbing Luo, Peng Lv, Leif Andersson, and Ning Li. “Patterns of East Asian Pig Domestication, Migration, and Turnover Revealed by Modern and Ancient DNA.” Proceedings of the National Academy of Sciences, United States 19 Apr. 2010. 10 Sep. 2010 http://www.pnas.org/cgi/content/full/0912264107/DCSupplemental Meindertsma, Christien. “PIG 05049. Kunsthal in Rotterdam.” 2008. 10 Sep. 2010 http://www.christienmeindertsma.com/index.php?/books/pig-05049Naess, A. “The Shallow and the Deep, Long-Range Ecology Movement.” Inquiry 16 (1973): 95-100. Needman, T. Fat Pig. Sydney Theatre Company. Oct. 2006. Noonan, Chris [director]. “Babe (1995) Memorable Quotes”. 10 Sep. 2010 http://www.imdb.com/title/tt0112431/quotes Plumwood, V. Feminism and the Mastery of Nature. London: Routledge, 1993. Pulp Fiction. Dir. Quentin Tarantino. Miramax, 1994. RSPCA Tasmania. “RSPCA Calls for Ban on Intensive Pig Farming.” 10 Sep. 2010 http://www.rspcatas.org.au/press-centre/rspca-calls-for-a-ban-on-intensive-pig-farming ScienceDaily. “Ancient Pig DNA Study Sheds New Light on Colonization of Europe by Early Farmers” 4 Sep. 2007. 10 Sep. 2010 http://www.sciencedaily.com/releases/2007/09/070903204822.htm Singer, Peter. “Down on the Family Farm ... or What Happened to Your Dinner When it was Still an Animal.” Animal Liberation 2nd ed. London: Jonathan Cape, 1990. 95-158. Soler, Jean. “Biblical Reasons: The Dietary Rules of the Ancient Hebrews.” Food: A Culinary History. Eds. Jean-Louis Flandrin and Massimo Montanari. Trans. Clarissa Botsford, Arthus Golhammer, Charles Lambert, Frances M. López-Morillas and Sylvia Stevens. New York: Columbia University Press, 1999. 46-54. Watson, Lyall. The Whole Hog: Exploring the Extraordinary Potential of Pigs. London: Profile, 2004. White, E. B. Essays of E. B. White. London: HarperCollins, 1979. White, E. B. Charlotte’s Web. London: HarperCollins, 2004. Wright, J., and V. Harwood. Eds. Biopolitics and the ‘Obesity Epidemic’. New York: Routledge, 2009. Wykes, J. Fatuosity 2010. 29 Aug. 2010 http://www.fatuosity.net

IntroductionSydney’s Garden Palace was a magnificent building with a grandeur that dominated the skyline, stretching from the site of the current State Library of New South Wales to the building that now houses the Sydney Conservatorium of Music. The Palace captivated society from its opening in 1879. This article outlines the building of one of Sydney’s early structural icons and how, despite being destroyed by fire after three short years in 1882, it had an enormous impact on the burgeoning colonial community of New South Wales, thus building a physical structure, pride and a suite of memories.Design and ConstructionIn February 1878, the Colonial Secretary’s Office announced that “it is intended to hold under the supervision of the Agricultural Society of New South Wales an international Exhibition in Sydney in August 1879” (Official Record ix). By December the same year it had become clear that the Agricultural Society lacked the resources to complete the project and control passed to the state government. Colonial Architect James Barnet was directed to prepare “plans for a building suitable for an international exhibition, proposed to be built in the Inner Domain” (Official Record xx). Within three days he had submitted a set of drawings for approval. From this point on there was a great sense of urgency to complete the building in less than 10 months for the exhibition opening the following September.The successful contractor was John Young, a highly experienced building contractor who had worked on the Crystal Palace for the 1851 London International Exhibition and locally on the General Post Office and Exhibition Building at Prince Alfred Park (Kent 6). Young was confident, procuring electric lights from London so that work could be carried out 24 hours a day, to ensure that the building was delivered on time. The structure was built, as detailed in the Colonial Record (1881), using over 1 million metres of timber, 2.5 million bricks and 220 tonnes of galvanised corrugated iron. Remarkably the building was designed as a temporary structure to house the Exhibition. At the end of the Exhibition the building was not dismantled as originally planned and was instead repurposed for government office space and served to house, among other things, records and objects of historical significance. Ultimately the provisional building materials used for the Garden Palace were more suited to a temporary structure, in contrast with those used for the more permanent structures built at the same time which are still standing today.The building was an architectural and engineering wonder set in a cathedral-like cruciform design, showcasing a stained-glass skylight in the largest dome in the southern hemisphere (64 metres high and 30 metres in diameter). The total floor space of the exhibition building was three and half hectares, and the area occupied by the Garden Palace and related buildings—including the Fine Arts Gallery, Agricultural Hall, Machinery Hall and 10 restaurants and places of refreshment—was an astounding 14 hectares (Official Record xxxvi). To put the scale of the Garden Palace into contemporary perspective it was approximately twice the size of the Queen Victoria Building that stands on Sydney’s George Street today.Several innovative features set the building apart from other Sydney structures of the day. The rainwater downpipes were enclosed in hollow columns of pine along the aisles, ventilation was provided through the floors and louvered windows (Official Record xxi) while a Whittier’s Steam Elevator enabled visitors to ascend the north tower and take in the harbour views (“Among the Machinery” 70-71). The building dominated the Sydney skyline, serving as a visual anchor point that welcomed visitors arriving in the city by boat:one of the first objects that met our view as, after 12 o’clock, we proceeded up Port Jackson, was the shell of the Exhibition Building which is so rapidly rising on the Domain, and which next September, is to dazzle the eyes of the world with its splendours. (“A ‘Bohemian’s’ Holiday Notes” 2)The DomeThe dome of the Garden Palace was directly above the intersection of the nave and transept and rested on a drum, approximately 30 metres in diameter. The drum featured 36 oval windows which flooded the space below with light. The dome was made of wood covered with corrugated galvanised iron featuring 12 large lattice ribs and 24 smaller ribs bound together with purlins of wood strengthened with iron. At the top of the dome was a lantern and stained glass skylight designed by Messrs. Lyon and Cottier. It was light blue, powdered with golden stars with wooden ribs in red, buff and gold (Notes 6). The painting and decorating of the dome commenced just one month before the exhibition was due to open. The dome was the sixth largest dome in the world at the time. During construction, contractor Mr Young allowed visitors be lifted in a cage to view the building’s progress.During the construction of the Lantern which surmounts the Dome of the Exhibition, visitors have been permitted, through the courtesy of Mr. Young, to ascend in the cage conveying materials for work. This cage is lifted by a single cable, which was constructed specially of picked Manilla hemp, for hoisting into position the heavy timbers used in the construction. The sensation whilst ascending is a most novel one, and must resemble that experienced in ballooning. To see the building sinking slowly beneath you as you successively reach the levels of the galleries, and the roofs of the transept and aisles is an experience never to be forgotten, and it seems a pity that no provision can be made for visitors, on paying a small fee, going up to the dome. (“View from the Lantern of the Dome Exhibition” 8)The ExhibitionInternational Exhibitions presented the opportunity for countries to express their national identities and demonstrate their economic and technological achievements. They allowed countries to showcase the very best examples of contemporary art, handicrafts and the latest technologies particularly in manufacturing (Pont and Proudfoot 231).The Sydney International Exhibition was the ninth International Exhibition and the colony’s first, and was responsible for bringing the world to Sydney at a time when the colony was prosperous and full of potential. The Exhibition—opening on 17 September 1879 and closing on 20 April 1880—had an enormous impact on the community, it boosted the economy and was the catalyst for improving the city’s infrastructure. It was a great source of civic pride.Image 1: The International Exhibition Sydney, 1879-1880, supplement to the Illustrated Sydney News Jan. 1880. Image credit: Mitchell Library, State Library of NSW (call no.: DL X8/3)This bird’s eye view of the Garden Palace shows how impressive the main structure was and how much of the Gardens and Domain were occupied by ancillary buildings for the Exhibition. Based on an original drawing by John Thomas Richardson, chief engraver at the Illustrated Sydney News, this lithograph features a key identifying buildings including the Art Gallery, Machinery Hall, and Agricultural Hall. Pens and sheds for livestock can also be seen. The parade ground was used throughout the Exhibition for displays of animals. The first notable display was the International Show of Sheep featuring Australian, French and English sheep; not surprisingly the shearing demonstrations proved to be particularly popular with the community.Approximately 34 countries and their colonies participated in the Exhibition, displaying the very best examples of technology, industry and art laid out in densely packed courts (Barnet n.p.). There were approximately 14,000 exhibits (Official Record c) which included displays of Bohemian glass, tapestries, fine porcelain, fabrics, pyramids of gold, metals, minerals, wood carvings, watches, ethnographic specimens, and heavy machinery. Image 2: “Meet Me under the Dome.” Illustrated Sydney News 1 Nov. 1879: 4. Official records cite that between 19,853 and 24,000 visitors attended the Exhibition on the opening day of 17 September 1879, and over 1.1 million people visited during its seven months of operation. Sizeable numbers considering the population of the colony, at the time, was just over 700,000 (New South Wales Census).The Exhibition helped to create a sense of place and community and was a popular destination for visitors. On crowded days the base of the dome became a favourite meeting place for visitors, so much so that “meet me under the dome” became a common expression in Sydney during the Exhibition (Official Record lxxxiii).Attendance was steady and continuous throughout the course of the Exhibition and, despite exceeding the predicted cost by almost four times, the Exhibition was deemed a resounding success. The Executive Commissioner Mr P.A. Jennings remarked at the closing ceremony:this great undertaking […] marks perhaps the most important epoch that has occurred in our history. In holding this exhibition we have entered into a new arena and a race of progress among the nations of the earth, and have placed ourselves in kindly competition with the most ancient States of the old and new world. (Official Record ciii)Initially the cost of admission was set at 5 shillings and later dropped to 1 shilling. Season tickets for the Exhibition were also available for £3 3s which entitled the holder to unlimited entry during all hours of general admission. Throughout the Exhibition, season ticket holders accounted for 76,278 admissions. The Exhibition boosted the economy and encouraged authorities to improve the city’s services and facilities which helped to build a sense of community as well as pride in the achievement of such a fantastic structure. A steam-powered tramway was installed to transport exhibition-goers around the city, after the Exhibition, the tramway network was expanded and by 1905–1906 the trams were converted to electric traction (Freestone 32).After the exhibition closed, the imposing Garden Palace building was used as office space and storage for various government departments.An Icon DestroyedIn the early hours of 22 September 1882 tragedy struck when the Palace was engulfed by fire (“Destruction of the Garden Palace” 7). The building – and all its contents – destroyed.Image 3: Burning of the Garden Palace from Eaglesfield, Darlinghurst, sketched at 5.55am, Sep 22/82. Image credit: Mitchell Library, State Library of NSW (call no.: SSV/137) Many accounts and illustrations of the Garden Palace fire can be found in contemporary newspapers and artworks. A rudimentary drawing by an unknown artist held by the State Library of New South Wales appears to have been created as the Palace was burning. The precise time and location is recorded on the painting, suggesting it was painted from Eaglesfield, a school on Darlinghurst Road. It purveys a sense of immediacy giving some insight into the chaos and heat of the tragedy. A French artist living in Sydney, Lucien Henry, was among those who attempted to capture the fire. His assistant, G.H. Aurousseau, described the event in the Technical Gazette in 1912:Mister Henry went out onto the balcony and watched until the Great Dome toppled in; it was then early morning; he went back to his studio procured a canvas, sat down and painted the whole scene in a most realistic manner, showing the fig trees in the Domain, the flames rising through the towers, the dome falling in and the reflected light of the flames all around. (Technical Gazette 33-35)The painting Henry produced is not the watercolour held by the State Library of New South Wales, however it is interesting to see how people were moved to document the destruction of such an iconic building in the city’s history.What Was Destroyed?The NSW Legislative Assembly debate of 26 September 1882, together with newspapers of the day, documented what was lost in the fire. The Garden Palace housed the foundation collection of the Technological and Sanitary Museum (the precursor to the Powerhouse Museum, now the Museum of Applied Arts and Sciences), due to open on 1 December 1882. This collection included significant ethnological specimens such as Australian Indigenous artefacts, many of which were acquired from the Sydney International Exhibition. The Art Society of New South Wales had hung 300 paintings in preparation for their annual art exhibition due to open on 2 October of that year, all of these paintings consumed by fire.The Records of the Crown Lands Occupation Office were lost along with the 1881 Census (though the summary survived). Numerous railway surveys were lost, as were: £7,000 worth of statues, between 20,000 and 30,000 plants and the holdings of the Linnean Society offices and museum housed on the ground floor. The Eastern Suburbs Brass Band performed the day before at the opening of the Eastern Suburbs Horticultural Society Flower show; all the instruments were stored in the Garden Palace and were destroyed. Several Government Departments also lost significant records, including the: Fisheries Office; Mining Department; Harbour and Rivers Department; and, as mentioned, the Census Department.The fire was so ferocious that the windows in the terraces along Macquarie Street cracked with the heat and sheets of corrugated iron were blown as far away as Elizabeth Bay. How Did The Fire Start?No one knows how the fire started on that fateful September morning, and despite an official enquiry no explanation was ever delivered. One theory blamed the wealthy residents of Macquarie Street, disgruntled at losing their harbour views. Another was that it was burnt to destroy records stored in the basement of the building that contained embarrassing details about the convict heritage of many distinguished families. Margaret Lyon, daughter of the Garden Palace decorator John Lyon, wrote in her diary:a gentleman who says a boy told him when he was putting out the domain lights, that he saw a man jump out of the window and immediately after observed smoke, they are advertising for the boy […]. Everyone seems to agree on his point that it has been done on purpose – Today a safe has been found with diamonds, sapphires and emeralds, there were also some papers in it but they were considerably charred. The statue of her majesty or at least what remains of it, for it is completely ruined – the census papers were also ruined, they were ready almost to be sent to the printers, the work of 30 men for 14 months. Valuable government documents, railway and other plans all gone. (MLMSS 1381/Box 1/Item 2) There are many eyewitness accounts of the fire that day. From nightwatchman Mr Frederick Kirchen and his replacement Mr John McKnight, to an emotional description by 14-year-old student Ethel Pockley. Although there were conflicting accounts as to where the fire may have started, it seems likely that the fire started in the basement with flames rising around the statue of Queen Victoria, situated directly under the dome. The coroner did not make a conclusive finding on the cause of the fire but was scathing of the lack of diligence by the authorities in housing such important items in a building that was not well-secured a was a potential fire hazard.Building a ReputationA number of safes were known to have been in the building storing valuables and records. One such safe, a fireproof safe manufactured by Milner and Son of Liverpool, was in the southern corner of the building near the southern tower. The contents of this safe were unscathed in contrast with the contents of other safes, the contents of which were destroyed. The Milner safe was a little discoloured and blistered on the outside but otherwise intact. “The contents included three ledgers, or journals, a few memoranda and a plan of the exhibition”—the glue was slightly melted—the plan was a little discoloured and a few loose papers were a little charred but overall the contents were “sound and unhurt”—what better advertising could one ask for! (“The Garden Palace Fire” 5).barrangal dyara (skin and bones): Rebuilding CommunityThe positive developments for Sydney and the colony that stemmed from the building and its exhibition, such as public transport and community spirit, grew and took new forms. Yet, in the years since 1882 the memory of the Garden Palace and its disaster faded from the consciousness of the Sydney community. The great loss felt by Indigenous communities went unresolved.Image 4: barrangal dyara (skin and bones). Image credit: Sarah Morley.In September 2016 artist Jonathan Jones presented barrangal dyara (skin and bones), a large scale sculptural installation on the site of the Garden Palace Building in Sydney’s Royal Botanic Garden. The installation was Jones’s response to the immense loss felt throughout Australia with the destruction of countless Aboriginal objects in the fire. The installation featured thousands of bleached white shields made of gypsum that were laid out to show the footprint of the Garden Palace and represent the rubble left after the fire.Based on four typical designs from Aboriginal nations of the south-east, these shields not only raise the chalky bones of the building, but speak to the thousands of shields that would have had cultural presence in this landscape over generations. (Pike 33)ConclusionSydney’s Garden Palace was a stunning addition to the skyline of colonial Sydney. A massive undertaking, the Palace opened, to great acclaim, in 1879 and its effect on the community of Sydney and indeed the colony of New South Wales was sizeable. There were brief discussions, just after the fire, about rebuilding this great structure in a more permanent fashion for the centenary Exhibition in 1888 (“[From Our Own Correspondents] New South Wales” 5). Ultimately, it was decided that this achievement of the colony of New South Wales would be recorded in history, gifting a legacy of national pride and positivity on the one hand, but on the other an example of the destructive colonial impact on Indigenous communities. For many Sydney-siders today this history is as obscured as the original foundations of the physical building. What we build—iconic structures, civic pride, a sense of community—require maintenance and remembering. References“Among the Machinery.” The Sydney Mail and New South Wales Advertiser 10 Jan. 1880: 70-71.Aurousseau, G.H. “Lucien Henry: First Lecturer in Art at the Sydney Technical College.” Technical Gazette 2.III (1912): 33-35.Barnet, James. International Exhibition, Sydney, 1880: References to the Plans Showing the Space and Position Occupied by the Various Exhibits in the Garden Palace. Sydney: Colonial Architect’s Office, 1880.“A ‘Bohemian’s’ Holiday Notes.” The Singleton Argus and Upper Hunter General Advocate 23 Apr. 1879: 2.Census Department. New South Wales Census. 1881. 3 Mar. 2017 <http://hccda.ada.edu.au/pages/NSW-1881-census-02_vi>. “Destruction of the Garden Palace.” Sydney Morning Herald 23 Sep. 1882: 7.Freestone, Robert. “Space Society and Urban Reform.” Colonial City, Global City, Sydney’s International Exhibition 1879. Eds. Peter Proudfoot, Roslyn Maguire, and Robert Freestone. Darlinghurst, NSW: Crossing P, 2000. 15-33.“[From Our Own Correspondents] New South Wales.” The Age (Melbourne, Vic.) 30 Sep. 1882: 5.“The Garden Palace Fire.” Sydney Morning Herald 25 Sep. 1882: 5.Illustrated Sydney News and New South Wales Agriculturalist and Grazier 1 Nov. 1879: 4.“International Exhibition.” Australian Town and Country Journal 15 Feb. 1879: 11.Kent, H.C. “Reminiscences of Building Methods in the Seventies under John Young. Lecture.” Architecture: An Australian Magazine of Architecture and the Arts Nov. (1924): 5-13.Lyon, Margaret. Unpublished Manuscript Diary. MLMSS 1381/Box 1/Item 2.New South Wales, Legislative Assembly. Debates 22 Sep. 1882: 542-56.Notes on the Sydney International Exhibition of 1879. Melbourne: Government Printer, 1881.Official Record of the Sydney International Exhibition 1879. Sydney: Government Printer, 1881.Pike, Emma. “barrangal dyara (skin and bones).” Jonathan Jones: barrangal dyara (skin and bones). Eds. 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