Journal articles: 'Ruffier'

1

Заневський, І. П., and Л. Г. Заневська. "Ruffier test model taking into account an age of the patient." Teorìâ ta Metodika Fìzičnogo Vihovannâ, no. 2 (June 30, 2013): 17–27. http://dx.doi.org/10.17309/tmfv.2013.2.1013.

Full text

Abstract:

There are presented the results of the comparative analysis of the existing methods of correcting the values of Ruffier index for the children teenagers. The model of the correction of the value of Ruffier index and the model of the correction of the gradation of the estimations of Ruffier test taking into account an age of the patient are proposed. It is shown that the parameter of the correction of the value of Ruffier index and gradations of the estimations of the corresponding test must be a relationship of the standard pulse rate in the state of calmness, accepted for the age class of patient, to the standard pulse rate of adult person. The corresponding model of the estimation of the functional state of the cardiovascular system of persons of school age showed a statistically significal (p < 0,001) correction of the value of Ruffier index and the gradation of the levels of health relatively to the conventional model for the adult, which is recommended. The quantitative assessment of the consequences of correction in the distribution of students on levels and groups of health is represented.

APA, Harvard, Vancouver, ISO, and other styles

2

Raţă, Bogdan Constantin, Gloria Raţă, Marinela Raţă, and Costel Sava. "Assisted Detertermination of the Relation between the Ruffier and Margaria Kalamen Tests and the Human Running Test." Applied Mechanics and Materials 555 (June 2014): 640–45. http://dx.doi.org/10.4028/www.scientific.net/amm.555.640.

Full text

Abstract:

The Aim of this Research is to Underline the Relation between the Performance Results in 800m Events Achieved in Competitions and the Ruffier and Margaria-Kalamen Tests Assessed before the Competitions.Research Methods and Techniques. the Paper is a Constatative Experimental Study, of the Longitudinal Type, Performed on 10 Women Athletes Practising the 800m Running Event. the Realisation of this Study Started from Checking Three Hypotheses (the Sportswomen’s Results Improve from One Competition Season to another as a Result of the Continuously Performed Training Process; the Adaptability to Effort, Assessed by the Ruffier Test, Improves as a Result of the Sportswomen’s Continuous Training; the Value of the Sports Performances is Influenced by the Level of the Results at the Ruffier and Margaria-Kalamen Tests). The Subjects Involved in this Study are between 16 to 17 Years of Age. The Research Methods Used were the Following: Bibliographic Study, Testing, Statistical-Mathematical Method, Analysis, and for the Interpretation of Results we Used: the Arithmetic Mean, the Maximum Value and the Standard Deviation. as Assessment Events, we Used the Results Obtained during the Competition Seasons of Two Consecutive Years, Namely 2011 and 2012, a Test for Assessing the Effort Capacity Represented by the Ruffier Test and a Test for Assessing the Power of the Inferior Limbs Represented by the Margaria-Kalamen Test. Results are Presented in a Table and Contain the Performance Values, the Effort Adaptability Values and Power Development Values. they Underline the Efficiency of the Training Process and the Relation between the Sports Performances and the Values of the Indices of the Ruffier and Margaria Kalaman Tests. The Drawn Conclusions Underline the Fact that the Results in the 2012 Indoor Season and the Values of the Indices of the Ruffier and Margaria-Kalaman Tests are Better than those of the 2011 Season, but also the Fact that there is an Inter-Conditioning Relation. the Progress Rate was Different from One Athlete to another.

APA, Harvard, Vancouver, ISO, and other styles

3

Alahmari, Khalid A., Kanagaraj Rengaramanujam, Ravi Shankar Reddy, Paul Silvian Samuel, Venkata Nagaraj Kakaraparthi, Irshad Ahmad, and Jaya Shanker Tedla. "Cardiorespiratory Fitness as a Correlate of Cardiovascular, Anthropometric, and Physical Risk Factors: Using the Ruffier Test as a Template." Canadian Respiratory Journal 2020 (September 8, 2020): 1–10. http://dx.doi.org/10.1155/2020/3407345.

Full text

Abstract:

Background. Assessment of cardiorespiratory fitness (CRF) is a standard procedure in routine clinical practices. Early identification of risk factors through screening is vital in the fight against chronic diseases. Evaluation of CRF can impose cost implications in the clinical setting; thus, a simple and easy-to-use test is to be advocated. The Ruffier test is a simple test that can assess CRF, and it is necessary to find whether the test reflects the effects of compounding factors in CRF. Objective. This study aims to determine the association between CRF (estimated VO2max) with cardiovascular, anthropometric, and physical risk factors using the Ruffier test. Methods. A cross-sectional study with a sample of 52 male participants was conducted. Before the Ruffier test, each participant’s body weight, height, waist circumference, skinfold thickness, thigh length, lower-limb length, thigh circumference, physical activity, blood pressure, smoking, diabetes, and pulmonary functions were recorded, and these factors correlated with CRF. Results. There was a significant inverse relationship found between the estimated VO2max and age, height, body weight, body mass index, waist circumference, a sum of skinfold, fat percentage, thigh length, lower-limb length, thigh circumference, smoking, blood pressure, heart rates, and diabetes p<0.05. A significant positive correlation was found between the estimated VO2max with physical activity and respiratory functions p<0.05. In the multivariable model, body weight and resting heart rate were significantly inversely associated with the estimated VO2maxp<0.05. Conclusion. Using the Ruffier test, various risk factors of CRF are correlated with the estimated VO2max. This test reflects the effects of different compounding factors on CRF; therefore, it can be used in routine clinical practices to identify the risk factors early.

APA, Harvard, Vancouver, ISO, and other styles

4

Sinziana, К., and A. Silisteanu. "THE IMPORTANCE FOR YOUNG ADULTS TO DO PHYSICAL ACTIVITIES FOR A HEALTHY LIFESTYLE." Вісник Прикарпатського університету. Серія: Фізична культура, no. 33 (January 3, 2020): 84–89. http://dx.doi.org/10.15330/fcult.33.84-89.

Full text

Abstract:

The relationship between the physical activity and the health condition is very complex since the physical activity brings benefits to health whereas the movement is essential for the human body to work properly. The physical activity, the physical condition and the health condition are influenced by the genetic factors, environmental conditions and lifestyle of each individual. Objectives. The trial aimed at pointing out the cardiorespiratory functional status of students correlated to their lifestyle and physical activity level. Material and method. The cardio-respiratory capacity was tested on the students in the 1st year at the Department of Health and Human Development, precisely 30 students. The inclusion criteria were: their age of 18–40, their consent to take part in the trial, their compliance during the trial, their lack of acute conditions or chronic ones. The Ruffier test was used, a test that assesses the functional capacity of the respiratory and cardiovascular apparatus. Results. The interpretation of the Ruffier test and Ruffier-Dickson test enables an overall view about the ability to adapt the body to effort for the students taken into consideration for the trial. Conclusions. The results of the trial show us that the participants have a good physical condition as they do a series of physical exercises on a daily basis according to the study curriculum. These results are similar to the ones in the specialty literature where the pyramid of physical activities plays an important role

APA, Harvard, Vancouver, ISO, and other styles

5

Yaroshevich, Irina. "DETERMINATION OF PHYSICAL PERFORMANCE STUDENTS." Modern Technologies and Scientific and Technological Progress 1, no. 1 (May 17, 2021): 357–58. http://dx.doi.org/10.36629/2686-9896-2021-1-1-357-358.

Full text

Abstract:

The analysis of the work of the physical performance of the students of AnGTU with the help of functional tests of Ruffier for the study of general endurance and the Harvard step test is considered

APA, Harvard, Vancouver, ISO, and other styles

6

Zanevskyy, Ihor, and Lyudmyla Zanevska. "Approbation of the Ruffier Test Model Adapted for Children." Journal of Testing and Evaluation 46, no. 3 (November 21, 2017): 20170007. http://dx.doi.org/10.1520/jte20170007.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Szabóová, Renáta, Natália Škutová, and Robert Herich. "Can the Regular Physical Load Change the Functional State of Pupils in Elementary School?" Acta Facultatis Educationis Physicae Universitatis Comenianae 60, no. 2 (November 1, 2020): 146–57. http://dx.doi.org/10.2478/afepuc-2020-0012.

Full text

Abstract:

SummaryThe functional status of the organism is a cluster of aerobic, cardiorespiratory and cardiovascular ability in co-operation with the muscular ability ensuring physiological activity of myocard and muscles in the form of regular dynamic and static active movement. Nowadays is observed an increasing lack of physical activity in the pediatric population, which is reflected in the increasing number of the civilization diseases. The aim of this work was to show the impact of regular dynamic activity on the functional status of the cardiovascular system and fitness in schoolable children (n = 100) using Ruffier’s functional test. The values of the Ruffier index were monitored and compared in groups of girls (n = 25) and boys (n = 25) involved in regular school activity within subject „Physical education“ in comparison with groups of children participating also in regular out-of-school physical activity (girls n = 25, boys n = 25) during 5 months. The most significant result (p < 0.001) was observed in functional status of children involved in both regular and after-school activity after 5 months. Presented work showed the significant impact of regular physical activities on circulatory system and muscular work of schoolable children.

APA, Harvard, Vancouver, ISO, and other styles

8

Cherkesov, Timur, Cherim Ingushev, Anna Konopleva, Radmir Cherkessov, Magomed Gairbekov, and Azamat Zhukov. "Features of the performance exposure in girls involved in cyclic and acyclic sports." Journal of Medicine and Life 14, no. 1 (January 2021): 105–10. http://dx.doi.org/10.25122/jml-2020-0054.

Full text

Abstract:

According to the definition adopted by the international biological program, physical performance is characterized by maximum oxygen consumption (MOC). Profession, lifestyle, and sport affect the value of the MOC. For anatomy and physiology, oxygen delivery to muscle tissue depends on the state of the respiratory and cardiovascular system, the amount and composition of blood. In this case, the leading role belongs to the cardiac activity, namely to the magnitude of the shock and minute volumes of blood in working conditions. High values of MOC and, consequently, a large work capacity are characteristic of athletes specializing in cyclic sports with moderate and high power. The purpose of the research was to evaluate the adaptive response of the cardiovascular system of girls involved in cyclic and acyclic sports as well as those not involved in sports. The study was conducted in 2018. During the research, we used pulse oximetry and determination of blood pressure according to N.S. Korotkov, as well as an assessment of the adaptation of the cardiovascular system according to the Ruffier Index. Studies have shown differences in the level of performance of girls involved in cyclic sports (athletics) and acyclic sports (karate and taekwondo), as well as non-sports. During the experiments, heart rate and blood pressure indicators were recorded at rest and after exercise, and the Ruffier Index, which reflects the level of performance of the participants, was calculated.

APA, Harvard, Vancouver, ISO, and other styles

9

Chernenko, S., O. Oliinyk, O. Kapkan, and Zh Malakhova. "Peculiarities of Higher Education Students’ Functional and Motor Preparedness." Teorìâ ta Metodika Fìzičnogo Vihovannâ 17, no. 4 (December 30, 2017): 169–76. http://dx.doi.org/10.17309/tmfv.2017.4.1201.

Full text

Abstract:

The objective is to determine the age peculiarities of higher education students’ functional and motor preparedness.Materials and methods. The participants in the study were male students of the 1st year (n = 98), 2nd year (n = 69), 3rd year (n = 56), 4th year (n = 32), and 5th year (n = 46) of training.The following research methods were used to solve the tasks set within the study: analysis of scientific literature, pedagogical observation, pedagogical testing; the index method and biomedical methods. The pedagogical methods were used to study the peculiarities of the functional state of the organism and the motor abilities in higher education students of the 1st-5th years of trtaining; methods of mathematical statistics were used to processing the data.Results. The first and second-year students demonstrated statistically reliable dynamics in the indicators of their functional and motor preparedness. Thus, at the age of 19, the students show a decrease in the Ruffier index (9.7 – “good heart”), the static equilibrium (12 sec. – “satisfactory”), and the standing long jump (Р <0.05). The breath-holding indicators and the speed-strength abilities (running 100 m) and the strength of hand muscles remain unchanged. At the age of 20, the students show a significant deterioration in the results of Gench’s test (31 sec.) and Romberg’s test (3.9 sec.), (P <0.05). The recorded results of the Ruffie index, timed inspiratory capacity, speed-strength abilities, and strength demonstrated no change. At the age of 20-21, significant changes happen to the indicators of the cardiovascular system and breath-holding. The speed-strength abilities, the strength of hand muscles remain unchanged. At the age of 21-22 (4th and 5th year), all the indicators show no change.Conclusions. At the age ranging between 18 and 22, the Ruffle index shows an increase in the number of students whose diovascular system state indicator is assessed as “good” and a decrease in the number of students whose result by this indicator is assessed as “bad”. The level of motor abilities development varies statistically unreliably.

APA, Harvard, Vancouver, ISO, and other styles

10

Modave, Francois, Trevor Leavitt, Ravi Kumar, Bian Jiang, Yi Guo, and Heather K. Vincent. "Relationship Between the Ruffier Test and Maximal Treadmill Testing in Healthy Adults." Medicine & Science in Sports & Exercise 49, no. 5S (May 2017): 600. http://dx.doi.org/10.1249/01.mss.0000518570.95524.e0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Zanevskyy, I., R. Janiszewska, and L. Zanevska. "Validity of Ruffier Test in Evaluation of Resistance to the Physical Effort." Journal of Testing and Evaluation 45, no. 6 (February 17, 2017): 20160380. http://dx.doi.org/10.1520/jte20160380.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Laure, P. "Indice de Ruffier: la fréquence cardiaque à 45 secondes n'est pas maximale." Science & Sports 11, no. 2 (January 1996): 124–26. http://dx.doi.org/10.1016/0765-1597(96)88160-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Tudor, Virgil, Florin Pelin, Gabriel Iulian Ghitescu, Alina-Daniela Moanta, Vasilica Grigore, Iozsef Hidi, Ana-Maria Mujea, et al. "Somato-Functional Profile of Children at Risk of Educational and Social Exclusion in Western Romania." BRAIN. BROAD RESEARCH IN ARTIFICIAL INTELLIGENCE AND NEUROSCIENCE 11, no. 4Sup1 (December 28, 2020): 200–222. http://dx.doi.org/10.18662/brain/11.4sup1/165.

Full text

Abstract:

This paper aims to identify the somatic and functional profile of middle-school students from three areas of Romania (South-West, West and North-West). A group of 364 students (173 girls and 191 boys) aged between 10 and 16 years (12.75 ± 1.2) from 6 different schools in Mehedinti, Caras-Severin and Bistrita-Nasaud counties took part in this study. The target group category includes Roma ethnic students, students from disorganised families and students at high risk of early school dropout, from both urban and rural areas. The calculated statistical indicators were: arithmetic mean, standard deviation, coefficient of variation and Pearson coefficient. The anthropometric measurements made to identify the somatic and functional profile of students were height, weight, BMI, arm span and Ruffier test. The results show an upward trend in the height of boys aged between 10 (1.44 m) and 16 years (1.67 m), the maximum value recorded for girls aged 14 years being 1.59 m. The highest value of body weight was identified in boys aged 15 years (58.91 kg) and girls aged 14 years (52.63 kg). The highest value for arm span was found for 15-years-old boys (167 cm) and 14-years-old girls (158.83 cm). BMI values for both boys and girls were between 17.22 and 21.52 points. In Ruffier test, the scores obtained by students ranged between 9.95 and 13.4. This research is part of the “Sustainable social and education integration through sport activities” project (PNP001).

APA, Harvard, Vancouver, ISO, and other styles

14

Tudor, Virgil, Florin Pelin, Gabriel Iulian Ghitescu, Alina-Daniela Moanta, Vasilica Grigore, Iozsef Hidi, Ana-Maria Mujea, et al. "Somato-Functional Profile of Children at Risk of Educational and Social Exclusion in Western Romania." BRAIN. BROAD RESEARCH IN ARTIFICIAL INTELLIGENCE AND NEUROSCIENCE 11, no. 4Sup1 (December 28, 2020): 200–222. http://dx.doi.org/10.18662/brain/11.4sup1/165.

Full text

Abstract:

This paper aims to identify the somatic and functional profile of middle-school students from three areas of Romania (South-West, West and North-West). A group of 364 students (173 girls and 191 boys) aged between 10 and 16 years (12.75 ± 1.2) from 6 different schools in Mehedinti, Caras-Severin and Bistrita-Nasaud counties took part in this study. The target group category includes Roma ethnic students, students from disorganised families and students at high risk of early school dropout, from both urban and rural areas. The calculated statistical indicators were: arithmetic mean, standard deviation, coefficient of variation and Pearson coefficient. The anthropometric measurements made to identify the somatic and functional profile of students were height, weight, BMI, arm span and Ruffier test. The results show an upward trend in the height of boys aged between 10 (1.44 m) and 16 years (1.67 m), the maximum value recorded for girls aged 14 years being 1.59 m. The highest value of body weight was identified in boys aged 15 years (58.91 kg) and girls aged 14 years (52.63 kg). The highest value for arm span was found for 15-years-old boys (167 cm) and 14-years-old girls (158.83 cm). BMI values for both boys and girls were between 17.22 and 21.52 points. In Ruffier test, the scores obtained by students ranged between 9.95 and 13.4. This research is part of the “Sustainable social and education integration through sport activities” project (PNP001).

APA, Harvard, Vancouver, ISO, and other styles

15

Нечитайло, Y., and B. Othmen Mabrouk. "Peculiarities of breath holding tests in school age children." Bukovinian Medical Herald 25, no. 1(97) (May 26, 2021): 103–7. http://dx.doi.org/10.24061/2413-0737.xxv.1.97.2021.15.

Full text

Abstract:

Objective of the study was to examine the performance of breath-holding and Ruffier tests, relationship between them and other factors in healthy school age children.Material and methods. The cross-sectional descriptive study with 45 healthy participants in age 7-13 years (22 boys and 23 girls) was performed. The study included assessment of nutrition, physical activity habits, family and socioeconomic data, resting anthropometric and blood pressure measurements, pulse oximetry, tests for the functional status of the cardiorespiratory system and readiness of child organism for physical loading (Ruffier and volitional breath-holding hypoxic tests). Results. The Rufier index in most children was average and amounted to 8.75±0.32 units. Test with breath-holding on inspiration was at the level of 37.0±1.84 sec, on exhalation - 22.2±1.3 sec. In children with low tolerance to exercise during respiratory tests execution there were a decrease in blood saturation and changes in heart rate. The tests are based on different physiological processes in the human body that create physical load tolerance: heart tolerance and cardiorespiratory oxygen supply to tissues.Conclusions. All selected tests could be executed anywhere and in a short period of time. They are easy to perform and do not cause emotional reactions in children and could be used for tolerance for physical loading assessment. The results obtained are complementary and can be recommended for use in the complex when examining the health and tolerance for physical loading in children during different screening types.

APA, Harvard, Vancouver, ISO, and other styles

16

Maliar, N., E. Maliar, A. Ognystyy, and K. Ognysta. "Implementation of the experimental methodology of preventive physical education in higher education institutions." Scientific Journal of National Pedagogical Dragomanov University. Series 15. Scientific and pedagogical problems of physical culture (physical culture and sports), no. 2(122) (February 21, 2020): 106–12. http://dx.doi.org/10.31392/npu-nc.series15.2020.2(122).21.

Full text

Abstract:

In this paper the content of the experimental method of preventive physical education, which was directed to solving the problems of primary prevention of diseases of the respiratory and cardiovascular systems of students, is disclosed; the content of the concept of "preventive physical education" is specified; the efficiency of complex use of preventive physical education in improving and preventive work with students by fixing during the experiment the average morbidity, lung capacity (LC), adaptive potential (AP) according to the method of RM. Baevsky and physical performance index (Ruffier test, IR).

APA, Harvard, Vancouver, ISO, and other styles

17

Rodríguez Cabrero, M., A. García Aparicio, T. García Pastor, J. J. Salinero, B. Pérez González, J. J. Sánchez Fernández, R. Gracia, S. Robledo, and R. Ibáñez Moreno. "Actividad física y ocio y su relación con el índice de Ruffier en adolescentes / Physical Activity And Leisure Habits And Relation With Ruffier Index In Adolescents pp. 165-180." RIMCAFD 57, no. 2015 (2015): 165–80. http://dx.doi.org/10.15366/rimcafd2015.57.012.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Mendez, A. Delgado, C. Bernardeau, and G. Pérès. "La pression artérielle lors de l'épreuve de ruffier et lors de l'épreuve sur ergocycle." Science & Sports 6, no. 2 (July 1991): 85–90. http://dx.doi.org/10.1016/s0765-1597(05)80113-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Chernenko, S., O. Honcharenko, and S. Marchenko. "Informative Indicators of Functional and Motor Fitness of Students of Higher Education Institutions." Teorìâ ta Metodika Fìzičnogo Vihovannâ 19, no. 3 (September 25, 2019): 107–15. http://dx.doi.org/10.17309/tmfv.2019.3.01.

Full text

Abstract:

The study objective was to determine informative indicators of functional and motor fitness of 1st-5th year students. Materials and methods. The study involved male students of 1st year (n = 67), 2nd year (n = 66), 3rd year (n = 62), 4th year (n = 45), 5th year (n = 56). The following research methods were used to solve the tasks set: analysis of scientific literature, pedagogical observation, pedagogical testing; index method and medical-biological methods. Pedagogical methods were used to study the peculiarities of functional state of the body and motor abilities of 1st-5th year students of higher education institutions; factor analysis was used for data processing. Results. By the Ruffier index, the 1st-2nd year students have an average heart performance. The 3rd-5th year students have a satisfactory heart performance. By the results of the Stange and Genci tests, the 1st-5th year students are evaluated as healthy and fit. According to the Romberg test, the 1st and 5th year students show higher results than the 2nd, 3rd and 4th year students (p < 0.001; p < 0.001; p < 0.006, respectively). The 3rd year students have the lowest results. The test results are estimated as lower than normal. Conclusions. The analysis of motor and functional fitness of the 1st-5th year students showed that the first factor in their structure is the functional state of the respiratory system, the second factor is speed strength and strength fitness. The most informative tests are: 1st year – the Stange test (0.822) and Genci test (0.741); 2nd year – the Genci test (0.758), the Ruffier index has the lowest informativity (0.11); 3rd year – “Standing long jump” (0.741) and the Genci test (0.723); 4th year – the Stange test (0.927) and Genci test (0.810); 5th year – the Stange test (0.799).

APA, Harvard, Vancouver, ISO, and other styles

20

Oliveira, Roberto C., Mário C. Marques, Daniel A. Marinho, Júlio Martins, Marta Martins, and Aldo M. Costa. "Can The Ruffier-dickson Test Be Used For The Evaluation Of Training Level In Sportsmen?" Medicine & Science in Sports & Exercise 42 (May 2010): 573. http://dx.doi.org/10.1249/01.mss.0000385422.80568.0d.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Ayán Pérez, C., F. Reigosa Galáns, J. M. Cancela Carral, H. Rodríguez Barreiro, and I. Martínez-Lemos. "Test-retest reliability and convergent validity of the Ruffier Index in children under 12 years old." Science & Sports 33, no. 6 (December 2018): 353–60. http://dx.doi.org/10.1016/j.scispo.2018.05.002.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

TUMBLETY, JOAN. "Rhetoric, Enterprise and Professional Authority: Dr James-Edward Ruffier and Physician-Writers in Twentieth-Century France." History 104, no. 359 (January 2019): 125–48. http://dx.doi.org/10.1111/1468-229x.12725.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Molano Tobar, Nancy Janneth, Dolly Ximena Molano Tobar, and Raquel Amalia Vélez Tobar. "Variación del componente antropométrico y parámetros cardiovasculares de jóvenes universitarios entre 2013 y 2016 en Popayán-Colombia." Revista Investigaciones Andina 21, no. 38 (March 30, 2019): 9–22. http://dx.doi.org/10.33132/01248146.990.

Full text

Abstract:

Objetivo. Identificar la variación en el tiempo de algunos parámetros antropométricos y cardiovasculares relacionados con la salud en jóvenes de una Institución de Educación Superior (IES) en Popayán-Colombia en el periodo comprendido entre 2013 a 2016. Métodos. Estudio cuantitativo de tipo descriptivo comparativo de carácter longitudinal, con una muestra intencional de 142 estudiantes universitarios, conformada por 70 hombres y 72 mujeres con un promedio de edad de 19,65 años±1,66, a quienes se les determinó el IMC, perímetros de cintura y cadera, test de Ruffier Dickson y valoración de la presión arterial. Resultados. Hay cambios entre 2013 a 2016 en los universitarios, relacionados con el incremento de sobrepeso y obesidad, presencia de pre hipertensión arterial y baja resistencia cardiovascular ante el ejercicio, con correlaciones significativas para el sexo femenino. Conclusiones. La vida universitaria genera detrimentos en la salud cardiovascular como incremento de sobrepeso, lo que demerita seguimiento para prevenir enfermedades futuras.

APA, Harvard, Vancouver, ISO, and other styles

24

Pashkevych, S. A., and G. M. Danilenko. "Comparative Evaluation of de Ruffier-Dickson and Martine's Tests of 2-d Year of Study Young School Children." Ukraïnsʹkij žurnal medicini, bìologìï ta sportu 1, no. 2 (May 19, 2016): 151–55. http://dx.doi.org/10.26693/jmbs01.02.151.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Piquet, L., F. Dalmay, J. C. Vandroux, and L. Pourcelot. "Doppler study of beta-blockade effects on heart-rate and muscular blood flow velocity after Ruffier-Dickson test." European Journal of Ultrasound 7 (February 1998): S20. http://dx.doi.org/10.1016/s0929-8266(97)80179-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Legg, John A., Guillaume Bompard, John Dawson, Hannah L. Morris, Natalie Andrew, Lisa Cooper, Simon A. Johnston, Giorgos Tramountanis, and Laura M. Machesky. "N-WASP Involvement in Dorsal Ruffle Formation in Mouse Embryonic Fibroblasts." Molecular Biology of the Cell 18, no. 2 (February 2007): 678–87. http://dx.doi.org/10.1091/mbc.e06-06-0569.

Full text

Abstract:

The Wiskott–Aldrich syndrome protein (WASP) family activates the Arp2/3 complex leading to the formation of new actin filaments. Here, we study the involvement of Scar1, Scar2, N-WASP, and Arp2/3 complex in dorsal ruffle formation in mouse embryonic fibroblasts (MEFs). Using platelet-derived growth factor to stimulate circular dorsal ruffle assembly in primary E13 and immortalized E9 Scar1+/+and Scar1 null MEFs, we establish that Scar1 loss does not impair the formation of dorsal ruffles. Reduction of Scar2 protein levels via small interfering RNA (siRNA) also did not affect dorsal ruffle production. In contrast, wiskostatin, a chemical inhibitor of N-WASP, potently suppressed dorsal ruffle formation in a dose-dependent manner. Furthermore, N-WASP and Arp2 siRNA treatment significantly decreased the formation of dorsal ruffles in MEFs. In addition, the expression of an N-WASP truncation mutant that cannot bind Arp2/3 complex blocked the formation of these structures. Finally, N-WASP−/−fibroblast-like cells generated aberrant dorsal ruffles. These ruffles were highly unstable, severely depleted of Arp2/3 complex, and diminished in size. We hypothesize that N-WASP and Arp2/3 complex are part of a multiprotein assembly important for the generation of dorsal ruffles and that Scar1 and Scar2 are dispensable for this process.

APA, Harvard, Vancouver, ISO, and other styles

27

Hasegawa, T. "Production of a unique antibody specific for membrane ruffles and its use to characterize the behavior of two distinct types of ruffles." Journal of Cell Biology 120, no. 6 (March 15, 1993): 1439–48. http://dx.doi.org/10.1083/jcb.120.6.1439.

Full text

Abstract:

I have produced a new monoclonal antibody, YF-169, against membrane ruffle specific 55-kD protein. YF-169 stained membrane ruffles of chick embryo fibroblasts so definitely that it enabled clear and reliable analyses of membrane ruffles. Fibroblasts organized two distinct types of membrane ruffles. One type of the ruffles were transiently formed in serum-starved cells (Type I) when stimulated by serum or platelet-derived growth factor. After spontaneous degradation of Type I ruffles, the other type of ruffles containing many microspikes were gradually organized at leading edges (Type II). The formation of Type I ruffles was not affected by either nocodazole, a microtubule-disrupting drug, or taxol, a microtubule-stabilizing reagent. However, Type II ruffles were entirely destroyed not only by nocodazole but also by taxol, suggesting that regulated organization of microtubule network is important to maintain Type II ruffles. H8, a protein kinase inhibitor prevented the spontaneous degradation of Type I ruffles and also reduced the destructive effect of nocodazole on Type II ruffles without affecting microtubule-disrupting activity. Protein kinases may be involved in the degradation processes of both types of ruffles. W7, a calmodulin antagonist, strongly inhibited Type I ruffle formation and completely destroyed Type II ruffles. W7 was also found to induce a remarkable change of 55-kD protein localization. After degradation of Type II ruffles, most of 55-kD protein was incorporated into newly formed unusual thick fibers. These results suggest that regulated organization of microtubule network is not necessary to form Type I ruffles but is important to maintain Type II ruffles, while calmodulin function is essential for both types of membrane ruffles.

APA, Harvard, Vancouver, ISO, and other styles

28

Patel, Prerna C., and Rene E. Harrison. "Membrane Ruffles Capture C3bi-opsonized Particles in Activated Macrophages." Molecular Biology of the Cell 19, no. 11 (November 2008): 4628–39. http://dx.doi.org/10.1091/mbc.e08-02-0223.

Full text

Abstract:

A widespread belief in phagocyte biology is that FcγR-mediated phagocytosis utilizes membrane pseudopods, whereas Mac-1–mediated phagocytosis does not involve elaborate plasma membrane extensions. Here we report that dynamic membrane ruffles in activated macrophages promote binding of C3bi-opsonized particles. We identify these ruffles as components of the macropinocytosis machinery in both PMA- and LPS-stimulated macrophages. C3bi-particle capture is facilitated by enrichment of high-affinity Mac-1 and the integrin-regulating protein talin in membrane ruffles. Membrane ruffle formation and C3bi-particle binding are cytoskeleton dependent events, having a strong requirement for F-actin and microtubules (MTs). MT disruption blunts ruffle formation and PMA- and LPS-induced up-regulation of surface Mac-1 expression. Furthermore, the MT motor, kinesin participates in ruffle formation implicating a requirement for intracellular membrane delivery to active membrane regions during Mac-1–mediated phagocytosis. We observed colocalization of Rab11-positive vesicles with CLIP-170, a MT plus-end binding protein, at sites of particle adherence using TIRF imaging. Rab11 has been implicated in recycling endosome dynamics and mutant Rab11 expression inhibits both membrane ruffle formation and C3bi-sRBC adherence to macrophages. Collectively these findings represent a novel membrane ruffle “capture” mechanism for C3bi-particle binding during Mac-1–mediated phagocytosis. Importantly, this work also demonstrates a strong functional link between integrin activation, macropinocytosis and phagocytosis in macrophages.

APA, Harvard, Vancouver, ISO, and other styles

29

Kornosenko, Oksana К., Iryna V. Taranenko, Yuliia V. Zaitseva, and Oleksandr V. Petryshyn. "COMPLEX ESTIMATION OF FITNESS TRAINING SYSTEMS AND WOMEN’S SOMATIC HEALTH OF THE FIRST PERIOD MATURE AGE." Wiadomości Lekarskie 73, no. 6 (2020): 1134–39. http://dx.doi.org/10.36740/wlek202006110.

Full text

Abstract:

The aim: To model, to ground and to check experimentally the efficiency of the complex organized system of fitness trainings and system of health improving trainings; to discover their influence on the status of women’s somatic health of the first period mature age. Materials and methods: Analysis, systematization, pedagogical observation, modelling, pedagogical experiment, tests: Ruffier, Stange, Romberg’s, Harvard step test and tests of PWC170. Results: Complex organized system of fitness trainings (dosed, systematic, complex motive activity, managed by trainer) appeared more effective (11.68 %) in comparison with system of individual health improving trainings (6.54 %). Both systems contributed to reducing of weight (CG2 – 3.8 %, EG2 – 16.3 %),decrease in body’s parts sizes, in particular, of breast (CG2 – 0.5 %, EG2 – 1.3 %), waist (CG2 – 2.3 %; EG2 – 13.75 %), pelvis (CG2 – 3.6 %; EG2 – 5.3 %), formation of physical characteristics (strength – CG2 – 2.1 %, EG2 – 17.4 % and flexibility – CG2 – 4.5 % and EG2 – 9 %).Differences in the level of physical capacity are marked, mainly, in the control group (PWC170absolute – 12.2 %; PWC170relative – 19.3 %), in comparison with experimental (PWC170 absolute – 10.2 %; PWC170 relative – 17.5 %). Conclusions: In general both systems assisted the improvement of somatic health level that proves the efficiency of system approach to organization of trainings in this agerelated group.

APA, Harvard, Vancouver, ISO, and other styles

30

Parfenova, L., G. Glazkova, and E. Gerasimov. "EVALUATION OF A SPORTS-SPECIFIC APPROACH IN PHYSICAL EDUCATION OF SECONDARY SCHOOL STUDENTS WITH HEALTH-RELATED ISSUES." Human Sport Medicine 20, no. 2 (July 14, 2020): 132–38. http://dx.doi.org/10.14529/hsm200216.

Full text

Abstract:

Aim. The article deals with the evaluation of experimental methods in the physical education of students with different nosologies based on a sports-specific approach. Materials and methods. The study involved 90 secondary school students (grades 5–6) with health-related issues. In the experimental group (EG, n = 45), training sessions were conducted according to the developed program that included volleyball and Russian lapta elements. In the control group (CG, n = 45), students were engaged in traditional activities for students of the special medical group (SMG). During the experiment, physical development, functional status, adaptive abilities, physical fitness, and physical education competencies were evaluated. Results. At the end of the experiment, the students of the experimental group showed a significant increase in the functional capacity of the body. The Shapovalova Index in EG improved by 8.90–21.70%, in CG – by 0.20–7.80%; the Ruffier Index in EG improved by 21.30–29.10%, in CG – by up to 7.80%. Moreover, students in EG had a more significant development of strength and coordination abilities than in CG. Conclusion. The experimental technique in physical education of secondary school students contributed to the development of physical abilities, health, and physical education competencies in participants.

APA, Harvard, Vancouver, ISO, and other styles

31

Cortesio, Christa L., Benjamin J. Perrin, David A. Bennin, and Anna Huttenlocher. "Actin-binding Protein-1 Interacts with WASp-interacting Protein to Regulate Growth Factor-induced Dorsal Ruffle Formation." Molecular Biology of the Cell 21, no. 1 (January 2010): 186–97. http://dx.doi.org/10.1091/mbc.e09-02-0106.

Full text

Abstract:

Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor–induced actin-based structures has not been defined. Here we show that mAbp1 localizes to dorsal ruffles and is necessary for platelet-derived growth factor (PDGF)-mediated dorsal ruffle formation. Despite their structural similarity, we find that mAbp1 and cortactin have nonredundant functions in the regulation of dorsal ruffle formation. mAbp1, like cortactin, is a calpain 2 substrate and the preferred cleavage site occurs between the actin-binding domain and the proline-rich region, generating a C-terminal mAbp1 fragment that inhibits dorsal ruffle formation. Furthermore, mAbp1 directly interacts with the actin regulatory protein WASp-interacting protein (WIP) through its SH3 domain. Finally, we demonstrate that the interaction between mAbp1 and WIP is important in regulating dorsal ruffle formation and that WIP-mediated effects on dorsal ruffle formation require mAbp1. Taken together, these findings identify a novel role for mAbp1 in growth factor–induced dorsal ruffle formation through its interaction with WIP.

APA, Harvard, Vancouver, ISO, and other styles

32

Leyton Román, Marta, Vicente Luis del Campo, Rafael Sabido Solana, and Jesús Morenas Martín. "Perfil y diferencias antropométricas y físicas de gimnastas de tecnificación de las modalidades de artística y rítmica (Anthropometric and physical differences of the gymnasts from the talent identification program of the artistic and rhythmic specialties." Retos, no. 21 (March 7, 2015): 58–62. http://dx.doi.org/10.47197/retos.v0i21.34606.

Full text

Abstract:

El objetivo del trabajo fue describir y comparar las características físicas y antropométricas de 25 gimnastas pertenecientes al grupo de tecnificación de la Federación Extremeña de Gimnasia. Estas gimnastas fueron clasificadas según modalidad gimnástica: artística femenina (GAF) (n=13) y gimnasia rítmica (GR) (n=12). Las variables dependientes incluyen valoraciones del porcentaje graso, a través de una báscula electrónica de columna con tallímetro (SECA 220cm); perímetros corporales, a través de un plicómetro (Holtain); diámetros y pruebas específicas de flexibilidad, a través de cinta métrica (CM 3m); frecuencia cardíaca, a través del test de Ruffier y uso de pulsómetro (Polar F6); fuerza isométrica del tren inferior, donde se utilizó una célula de carga (SSMAJ 5000N), y la capacidad de salto, a través de una plataforma de contacto (Lafayette CVP A73). Los resultados concluyen que GAF obtiene mejor resultado en las pruebas de flexibilidad (p<.05). Además, el grupo GR cuanto menor porcentaje graso posee mayor capacidad de salto SJ (r=-.774; p<.01) y CMJ (r=-.600; p<.05). En cambio, el grupo GAF cuanto mayor índice de masa corporal menor es su flexibilidad (p<.01). Se concluye que existen diferencias en composición corporal y pruebas físicas entre las modalidades gimnásticas femeninas de artística y rítmica, además de encontrar relaciones entre el rendimiento de dichas pruebas y variables antropométricas. Palabra clave: gimnasia, características antropométricas, flexibilidad, fuerza isométrica, salto.Abstract: The aim of our research was to describe the physical and anthropometric characteristics of 25 gymnasts from the talent identification program of the Extremeña Gymnastics Federation. Subjects were classified according to their specialty: women‘s artistic gymnastic (WAG) and rhythmic gymnastics (RG). The dependent variables include body fat percentage through an electronic scale column with stadiometer (SECA 220cm); body circumferences through a plicometer (Holtain); body diameters and specific tests of flexibility through a tape (CM 3m); a heart rate through Ruffier test and a heart rate monitor (Polar F6); isometric strength of lower extremities through a load cell (SSMAJ 5000N); and jumping ability through a contact mat (Lafayette CVP A73). The results concluded that the WAG group got the best results in flexibility tests (p<.05). Also, the RG group had lower body fat percentage and greater SJ jump ability (r=-.774; p<.01) and CMJ (r=-.600; p<.05). However, the WAG group showed a negative relation between body mass index and flexibility (p<.01). We conclude that there are differences in body composition and physical tests between the specialties/styles of women’s artistic gymnastics and female rhythmic gymnastics; we also found relationships between the performance of such tests and anthropometric variables.Key words: gymnastics, anthropometric characteristics, flexibility, isometric strength, jump.

APA, Harvard, Vancouver, ISO, and other styles

33

Robles-Romero, José, Eduardo Fernández-Ozcorta, Juan Gavala-González, Macarena Romero-Martín, Juan Gómez-Salgado, and Carlos Ruiz-Frutos. "Anthropometric Measures as Predictive Indicators of Metabolic Risk in a Population of “Holy Week Costaleros”." International Journal of Environmental Research and Public Health 16, no. 2 (January 13, 2019): 207. http://dx.doi.org/10.3390/ijerph16020207.

Full text

Abstract:

Preventive measures are a priority in those groups that perform intense physical efforts without physical preparation and that can also be overweight or obese. One of the groups that reflect these characteristics is the costaleros of the Holy Week of Andalusia, Spain. This paper aims to describe the effect of obesity on blood pressure. A descriptive cross-sectional study was conducted on 101 costaleros. The anthropometric measures were determined through segmental impedance. Cardiac recovery and anaerobic power were measured through the Ruffier–Dickson test and the Abalakov test, respectively. Blood pressure was measured when the individuals were at rest. The Kruskal–Wallis test was applied for of continuous parameters and the X2 test for dichotomous measures. Binary logistic regression models were used for the subsequent analysis with R-square and Receiver Operating Characteristic (ROC) curves. The average population was 28 years of age, 173.7 cm tall, and 82.59 Kg weigh. The excess of body fat was 11.27 Kg and Body Mass Index was 27.33 Kg/m2. 72.3% showed abnormal blood pressure and 68.2% were overweight. 32.7% had a waist-hip ratio higher than 0.94. The probability of presenting abnormal blood pressure was higher among the subjects whose fat content was higher and muscle content was lower.

APA, Harvard, Vancouver, ISO, and other styles

34

Felder, S., and E. L. Elson. "Mechanics of fibroblast locomotion: quantitative analysis of forces and motions at the leading lamellas of fibroblasts." Journal of Cell Biology 111, no. 6 (December 1, 1990): 2513–26. http://dx.doi.org/10.1083/jcb.111.6.2513.

Full text

Abstract:

Shapes, motions, and forces developed in lamellipodia and ruffles at the leading edges of primary chick embryo heart fibroblasts were characterized by differential interference contrast microscopy and digital video enhancement techniques. The initial extension of the cell edge to form a thin, planar lamellipodium parallel to the substrate surface was analyzed in two dimensions with temporal and spatial resolution of 3 s and 0.2 micron, respectively. An extension begins and ends with brief, rapid acceleration and deceleration separated by a long period of nearly constant velocity in the range of 4-7 microns/min. Extensions and retractions were initiated randomly over time. As demonstrated by optical sectioning microscopy, the extended lamellipodia formed ruffles by sharply bending upward at hinge points 2-4 microns behind their tips. Surprisingly, ruffles continued to grow in length at the same average rate after bending upward. They maintained a straight shape in vertical cross section, suggesting the ruffles were mechanically stiff. The forces required to bend ruffles of these cells and of BC3H1 cells were measured by pushing a thin quartz fishpole probe against the tip of a ruffle 7-10 microns from its base either toward or away from the center of the cell. Force was determined by measuring the bending of the probe monitored by video microscopy. Typically the probe forced the ruffle to swing rigidly in an arc about an apparent hinge at is base, and ruffles rapidly, and almost completely, recovered their shape when the probe was removed. Hence, ruffles appeared to be relatively stiff and to resist bending with forces more elastic than viscous, unlike the cell body. Ruffles on both types of cells resisted bending with forces of 15-30 mudyn/microns of displacement at their tips when pushed toward or away from the cell center. The significance of the observations for mechanisms of cell locomotion is discussed.

APA, Harvard, Vancouver, ISO, and other styles

35

Wang, Dawei, Ying-cai Tan, Geri E. Kreitzer, Yoko Nakai, Dandan Shan, Yi Zheng, and Xin-Yun Huang. "G Proteins G12 and G13 Control the Dynamic Turnover of Growth Factor-induced Dorsal Ruffles." Journal of Biological Chemistry 281, no. 43 (August 30, 2006): 32660–67. http://dx.doi.org/10.1074/jbc.m604588200.

Full text

Abstract:

Growth factors induce massive actin cytoskeletal remodeling in cells. These reorganization events underlie various cellular responses such as cell migration and morphological changes. One major form of actin reorganization is the formation and disassembly of dorsal ruffles (also named waves, dorsal rings, or circular ruffles). Dorsal ruffles are involved in physiological functions including cell migration, invasion, macropinocytosis, plasma membrane recycling, and others. Growth factors initiate rapid formation (within 5 min) of circular membrane ruffles, and these ruffles move along the dorsal side of the cells, constrict, close, and eventually disassemble (∼20 min). Considerable attention has been devoted to the mechanism by which growth factors induce the formation of dorsal ruffles. However, little is known of the mechanism by which these ruffles are disassembled. Here we have shown that G proteins G12 and G13 control the rate of disassembly of dorsal ruffles. In Gα12-/-Gα13-/- fibroblast cells, dorsal ruffles induced by growth factor treatment remain visible substantially longer (∼60 min) than in wild-type cells, whereas the rate of formation of these ruffles was the same with or without Gα12 and Gα13. Thus, Gα12/Gα13 critically regulate dorsal ruffle turnover.

APA, Harvard, Vancouver, ISO, and other styles

36

Gao, Ya-sheng, Charlotte C. Hubbert, Jianrong Lu, Yi-Shan Lee, Joo-Yong Lee, and Tso-Pang Yao. "Histone Deacetylase 6 Regulates Growth Factor-Induced Actin Remodeling and Endocytosis." Molecular and Cellular Biology 27, no. 24 (October 15, 2007): 8637–47. http://dx.doi.org/10.1128/mcb.00393-07.

Full text

Abstract:

ABSTRACT Histone deacetylase 6 (HDAC6) is a cytoplasmic deacetylase that uniquely catalyzes α-tubulin deacetylation and promotes cell motility. However, the mechanism underlying HDAC6-dependent cell migration and the role for microtubule acetylation in motility are not known. Here we show that HDAC6-induced global microtubule deacetylation was not sufficient to stimulate cell migration. Unexpectedly, in response to growth factor stimulation, HDAC6 underwent rapid translocation to actin-enriched membrane ruffles and subsequently became associated with macropinosomes, the vesicles for fluid-phase endocytosis. Supporting the importance of these associations, membrane ruffle formation, macropinocytosis, and cell migration were all impaired in HDAC6-deficient cells. Conversely, elevated HDAC6 levels promoted membrane ruffle formation with a concomitant increase in macropinocytosis and motility. In search for an HDAC6 target, we found that heat shock protein 90 (Hsp90), another prominent substrate of HDAC6, was also recruited to membrane ruffles and macropinosomes. Significantly, inhibition of Hsp90 activity suppressed membrane ruffling and cell migration, while expression of an acetylation-resistant Hsp90 mutant promoted ruffle formation. Our results uncover a surprising role for HDAC6 in actin remodeling-dependent processes and identify the actin cytoskeleton as an important target of HDAC6-regulated protein deacetylation.

APA, Harvard, Vancouver, ISO, and other styles

37

Wilfret, Gary J. "Florida Red Ruffles and Florida Irish Lace: Two New Lance-leaf Caladium Cultivars." HortScience 31, no. 4 (August 1996): 564. http://dx.doi.org/10.21273/hortsci.31.4.564.

Full text

Abstract:

Two lance-leaf caladium cultivars are to be released from the ornamental breeding program at the University of Florida. `Red Ruffles', whose pedigree is Red Frill × (`Red Frill' × `Candidum Jr.'), has elongated medium red leaves with ruffled green margins. Plants are upright with strong petioles, have leaf blades 25 cm long and 14 cm wide, and attain a height of 61 cm when grown in full sun in the field. Plants have more leaves and are more cold tolerant than `Red Frill', the major red lance-leaf cultivar of commerce. Tuber yields of `Red Ruffles' are similar to `Red Frill' but less than `Rosalie', with production indices of 95.0, 97.8, and 121.0, respectively. Foliage of `Red Ruffles' is more upright and less likely to elongate under reduced light than the other cultivars. `Irish Lace', an F2 selection from a cross of “Candidum Jr.' × “Red Frill', has elongated dark green leaves with heavily ruffled margins, which are etched with a thin red border. Leaf blades are 26 cm long and 8 cm wide and have heavy substance. Plant height is 65 cm in the field. Tuber yields of `Irish Lace' are greater than `White Wing', a major green/white cultivar. Use of a green caladium would be as a border or a mixture with red or white lance-leaf cultivars.

APA, Harvard, Vancouver, ISO, and other styles

38

Philip, Reuben V., and Rene E. Harrison. "A tent pole twist on membrane ruffles." Journal of Cell Biology 217, no. 11 (October 10, 2018): 3774–75. http://dx.doi.org/10.1083/jcb.201810022.

Full text

Abstract:

Macropinocytosis or “cell drinking” involves the elaboration of membrane ruffles that enclose and internalize extracellular fluids. Using lattice light sheet microscopy, Condon et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201804137) reveal the presence of parallel membrane protrusions termed “tent poles” that flank and direct membrane ruffle formation.

APA, Harvard, Vancouver, ISO, and other styles

39

Hasegawa, Junya, Emi Tokuda, Takeshi Tenno, Kazuya Tsujita, Haruko Sawai, Hidekazu Hiroaki, Tadaomi Takenawa, and Toshiki Itoh. "SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain." Journal of Cell Biology 193, no. 5 (May 30, 2011): 901–16. http://dx.doi.org/10.1083/jcb.201012161.

Full text

Abstract:

Reversible interactions between cytosolic proteins and membrane lipids such as phosphoinositides play important roles in membrane morphogenesis driven by actin polymerization. In this paper, we identify a novel lipid-binding module, which we call the SYLF domain (after the SH3YL1, Ysc84p/Lsb4p, Lsb3p, and plant FYVE proteins that contain it), that is highly conserved from bacteria to mammals. SH3YL1 (SH3 domain containing Ysc84-like 1) strongly bound to phosphatidylinositol 3,4,5-triphosphate (PI(3,4,5)P3) and several D5-phosphorylated phosphoinositides through its SYLF domain and was localized to circular dorsal ruffles induced by platelet-derived growth factor stimulation. Interestingly, SHIP2 (the PI(3,4,5)P3 5-phosphatase, src-homology 2–containing inositol-5-phosphatase 2) was identified as a binding partner of SH3YL1, and knockdown of these proteins significantly suppressed dorsal ruffle formation. Phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2), which is mainly synthesized from PI(3,4,5)P3 by the action of SHIP2, was enriched in dorsal ruffles, and PI(3,4)P2 synthesis strongly correlated with formation of the circular membrane structure. These results provide new insight into the molecular mechanism of dorsal ruffle formation and its regulation by phosphoinositide metabolism.

APA, Harvard, Vancouver, ISO, and other styles

40

Rymar, Olga, Alla Solovey, Nataliya Sorokolit, Uliana Shevtsiv, and Vasyl Matviiv. "TOOLS FOR CHILDREN FITNESS IN THE PHYSICAL EDUCATION OF PRIMARY SCHOOL PUPILS." SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference 3 (May 20, 2020): 540. http://dx.doi.org/10.17770/sie2020vol3.4852.

Full text

Abstract:

The results of pedagogical experiment that lasted from 2017 to 2018 are published in this research. 82 children took part in the experiment, among them were 42 boys and 40 girls. The actual age of children was nine years old. The purpose of the work was to discover the influence of fitness tools on physical development, physical preparation, psycho-emotional condition on younger school-age pupils. There was noticed a significant improvement of results of physical development according to Kettle index. In particular, it is about high level (from 16,9% to 43,1%), decreasing the average (from 54,4% to 46,8%) and especially the low level of physical development (from 28,7% to 10,1%),(p<0,05). Probably, positive changes appeared in the result of Ruffier Dickson tests, improvement indicators of intermediate level (from 4,8% to 14,1%),(p<0,05), decreasing in indicators of lower-intermediate level (from 38,4% to 16,7%),(p<0,05). The stable dynamics was noticed in the results of physical preparation (p<0,05), in particular after “30m run” exercise. Boys have got the result from 6,2±0,7sec to 5,4±0,5sec, girls - from 6,9±0,5sec to 6,1±0,5sec. The exercise “tilting torso forward from sitting position” represented result 3,9cm among boys and 5,5cm among girls; the exercise “long jump from place” represented 14,7cm among boys and 22,5cm among girls; in the exercise “shuttle running” boys got result from 11,7±1sec to 9,2±0,9sec and girls – from 12,1±1,2sec to 10,2±1 sec. What is more, positive and statistically important changes (p<0,05) where in results of pupils’ psycho-emotional condition evaluation. We have noticed increasing in levels of well-being, activeness, mood.

APA, Harvard, Vancouver, ISO, and other styles

41

Zebzeev, V., F. Zekrin, and O. Zdanovich. "FACTOR PROFILES OF FUNCTIONAL AND TECHNICAL FITNESS IN NORDIC COMBINED SKIERS DURING MULTIYEAR PREPARATION." Human Sport Medicine 19, S1 (August 17, 2019): 106–13. http://dx.doi.org/10.14529/hsm19s114.

Full text

Abstract:

Aim. The article deals with establishing the differences in the functional and technical fitness of Nordic combined skiers during multiyear preparation. Material and methods. To establish the factor profiles of the functional and technical fitness in Nordic combined skiers, we examined athletes at different stages of multiyear preparation: 40 athletes – initial preparation, 35 athletes – 2-year training program, 40 athletes – more than 2-year training program, 30 athletes – sports performance enhancement. The structure of functional and technical fitness was established with the help of factor analysis. Results. The comparative analysis of the factor structure of functional and technical fitness in Nordic combined skiers revealed the significant differences at each stage of multiyear training. It was established that during the stage of initial preparation and 2-year training program the most important factors are the nervous system (eye-hand visual reaction time, reaction to a moving object, interference resistance) and jumping fitness (angle characteristics during the run-up, take-off, flight, and landing), which create favorable conditions for forming the basics of ski-jumping technique in Nordic combined skiers. After 2-year training program and at the stage of performance enhancement the leading factors of physical fitness are determined by the cardiovascular (the Ruffier test, recovery constant, and Health integral indicator) and respiratory (VO2max, the Shtange's and Hench's breath test) systems, as well as ski racing technique (angle characteristics at different phases), which are favorable for the enhancement of ski racing technique. Conclusions. The results obtained allow the coaches to effectively improve functional and technical fitness and adjust training loads to the specifics of a certain stage of multiyear training.

APA, Harvard, Vancouver, ISO, and other styles

42

Lu, Xian-long, Zeng-zhen Qian, and Wen-zhi Yang. "Closure to “Discussion of ‘Axial Uplift Behavior of Belled Piers in Sloping Ground’ by F. A. B. Danziger, C. Pereira Pinto, A. P. Ruffier, and M. P. Pacheco”." Geotechnical Testing Journal 42, no. 2 (August 31, 2018): 20180048. http://dx.doi.org/10.1520/gtj20180048.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

GANDHI, Payal N., Richard M. GIBSON, Xiaofeng TONG, Jun MIYOSHI, Yoshimi TAKAI, Martha KONIECZKOWSKI, John R. SEDOR, and Amy L. WILSON-DELFOSSE. "An activating mutant of Rac1 that fails to interact with Rho GDP-dissociation inhibitor stimulates membrane ruffling in mammalian cells." Biochemical Journal 378, no. 2 (March 1, 2004): 409–19. http://dx.doi.org/10.1042/bj20030979.

Full text

Abstract:

Rac1, a member of the Rho family of small GTP-binding proteins, is involved in the regulation of the actin cytoskeleton via activation of lamellipodia and membrane ruffle formation. RhoGDI (Rho-family-specific GDP-dissociation inhibitor) forms a complex with Rho proteins in the cytosol of mammalian cells. It not only regulates guanine nucleotide binding to Rho proteins, but may also function as a molecular shuttle to carry Rho proteins from an inactive cytosolic pool to the membrane for activation. These studies tested if RhoGDI is necessary for the translocation of Rac1 from the cytosol to the plasma membrane for the formation of membrane ruffles. We describe a novel mutant of Rac1, R66E (Arg66→Glu), that fails to bind RhoGDI. This RhoGDI-binding-defective mutation is combined with a Rac1-activating mutation G12V, resulting in a double-mutant [Rac1(G12V/R66E)] that fails to interact with RhoGDI in COS-7 cells, but remains constitutively activated. This double mutant stimulates membrane ruffling to a similar extent as that observed after epidermal growth factor treatment of non-transfected cells. To confirm that Rac1 can signal ruffle formation in the absence of interaction with RhoGDI, Rac1(G12V) was overexpressed in cultured mesangial cells derived from a RhoGDI knockout mouse. Rac1-mediated membrane ruffling was indistinguishable between the RhoGDI(−/−) and RhoGDI(+/+) cell lines. In both the COS-7 and cultured mesangial cells, Rac1(G12V) and Rac1(G12V/R66E) co-localize with membrane ruffles. These findings suggest that interaction with RhoGDI is not essential in the mechanism by which Rac1 translocates to the plasma membrane to stimulate ruffle formation.

APA, Harvard, Vancouver, ISO, and other styles

44

Lozano Zapata, Rafael Enrique, Yusbely Cecilia Castrillón Jaimes, María Susana Peña Reyes, and Brian Johan Bustos Viviescas. "El esfuerzo físico y la salud en actividades recreativas y deportivas." Respuestas 23, S1 (July 1, 2018): 105–8. http://dx.doi.org/10.22463/0122820x.1528.

Full text

Abstract:

Antecedentes: El esfuerzo que se produce por la actividad física trae consigo múltiples beneficios, pero realizarlo de una formaprogramada, sistemática y con controles periódicos, se puede lograr observar los cambios que genera el ejerciciofisiológicamente, en pro de la salud y la condición física; cada fin de semana el ejercitarse en los espacios que ofrece la ciudad,la cantidad de personas y las diferentes actividades que se promueven para realizar ejercicio, deporte, arte y otras actividadespuede que no conlleven a realizar el esfuerzo físico requerido para mantener una óptima condición física. Objetivos: por estemotivo el estudio se enfoca en reconocer las necesidades de esta población que asiste a la ciclovía cada semana y poder orientarde una forma más apropiada a quienes no conocen de qué manera realizar una actividad física, en pro de su salud. Métodos: Elestudio presentó un enfoque mixto de tipo descriptivo donde se miden variables independientes el cual tuvo un diseño de campopara conocer los beneficios que trae realizar una fuerza y el ejercitarse como buena condición física y de salud. Resultados: parael logro de este objetivo se realizó una encuesta estructurada en la cual participaron 222 hombres (30,98±14,24 años) y 234mujeres (28,47±12,24 años), además se aplicó el Test de Ruffier-Dickson (TRD) para el cuál se tomó la frecuencia cardiaca enreposo, al final y recuperación a 181 personas de las cuales 143 realizan actividad física regularmente (Grupo AF) y 38 personasque no realizaban actividad física regularmente (Grupo NAF). Conclusiones: la muestra participante presentaba deficiencias enalgunos aspectos valorados, así mismo en la recolección de las valoraciones el beneficio del grupo AF presentó un IMC y % Gmenor que el grupo NAF puesto que en los demás datos ambos grupos obtuvieron valores muy similares. Palabras Claves: Actividad Física,Condición,Esfuerzo,Salud

APA, Harvard, Vancouver, ISO, and other styles

45

Kuchin, R., M. Stogov, N. Nenenko, N. Chernitsyna, and T. Maksimova. "DIFFERENCES IN PHYSICAL PREPAREDNESS OF DIFFERENT GENERATIONS OF FEMALES BORN BY THE MIGRANTS TO YUGRA." Human Sport Medicine 19, S2 (March 19, 2020): 74–80. http://dx.doi.org/10.14529/hsm19s210.

Full text

Abstract:

Aim. The purpose of the article is to study the characteristics of changes in physiological parameters and physical preparedness of different generations of females born by migrants to the Khanty-Mansi Autonomous District–Yugra and living there. Materials and methods. The study involved 60 females of which three groups were formed. Group 1 (n = 20) – females born in the regions of the middle zone of the Russian Federation who moved to the Khanty-Mansi Autonomous District-Yugra no more than 8 months ago. Group 2 (n = 20) – females born and permanently living in the Khanty–Mansi Autonomous District–Yugra, who are children (first generation) of migrants from the regions of the middle zone of the Russian Federation. Group 3 (n = 20) females born and permanently living in the Khanty-Mansi Autonomous Okrug-Yugra, who are grandchildren (second generation) of migrants from the regions of the middle zone of the Russian Federation. All subjects were comparable in age. A comprehensive examination was carried out, including an assessment of physical preparedness, anthropometry, physiological parameters, physical activity. Results. No significant differences between the average values of anthropometric indicators were found in the examined subjects. In girls of groups 2 and 3 compared to group 1, a statistically significant increase in variation in body weight, body mass index and chest circumference was found. In group 3, significant differences were noted in the indicators of strength and speed-strength endurance, as well as speed and coordination of movement. The girls of group 1 had a significantly increased value of the Ruffier index compared to other groups. Conclusion. Group 1 had a lower training status of the cardiovascular system and adaptation to living conditions compared to groups 2 and 3. The level of physical preparedness was lower in the subjects of group 3.

APA, Harvard, Vancouver, ISO, and other styles

46

Ovcharuk, Vasyl, Borys Maksymchuk, Vira Ovcharuk, Oleksandr Khomenko, Serhii Khomenko, Yevhen Yevtushenko, Petro Rybalko, et al. "Forming Competency in Health Promotion in Technical Specialists Using Physical Education." Revista Romaneasca pentru Educatie Multidimensionala 13, no. 3 (August 13, 2021): 01–19. http://dx.doi.org/10.18662/rrem/13.3/437.

Full text

Abstract:

In the light of new information, environmental, educational and production conditions, the problem of creating a health-promoting environment in a technical university appears to be rather important and needs to be studied in detail. The research aims to develop, justify and experimentally verify pedagogical conditions for forming competency in health promotion in technical students using physical education. The experiment involved two groups of students (Years 1-4): 241 individuals in the experimental group (EG) and 237 individuals in the control group (CG). Research methods include pedagogical observations, surveys, tests, self-evaluation, expert evaluation of students? practical skills, analysis of personal valeological and sport diaries of students; modelling in the framework of ascertaining and formative stages of the experiment; quantitative analysis of empirical data and its quality presentation; statistical verification of the proposed pedagogical conditions and the research hypothesis, the use of relevant tests (the Ruffier-Dickson index, the Cooper test) and quantitative indicators (Student?s t-test) in order to obtain some objective data on the results from the implementation of the author?s model. At the end of the experiment, most respondents (80 %) in the EG have reached a high level of competency in health promotion using physical education. In the CG, the differences in levels have not shown any significant changes since only 32.5% of respondents have a high level. Therefore, the results of the pedagogical experiment show some positive dynamics and confirm the effectiveness of the designed model and technology for forming competency in health promotion in technical students using physical education, which has made it possible to create qualitatively new conditions of the educational process and enhance students? motivation towards developing and realizing values in this field.

APA, Harvard, Vancouver, ISO, and other styles

47

Kang, Guo Jun, Chao Song, and Xue Feng Ren. "Effect of Meso-Submissions to Electronic Structure and Optical Properties of Ruffled Si Porphyrins Relatives." Advanced Materials Research 1028 (September 2014): 7–13. http://dx.doi.org/10.4028/www.scientific.net/amr.1028.7.

Full text

Abstract:

A series of silicon (VI) porphyrins compounds with varying meso substitutions Si (TPP)Cl2 (where X=5,10,15,20-tetraphenylporphyrin), Si (TFP)Cl2 (X=5,10,15,20-tetrafluorenylporphyrin), Si (TQP)Cl2(X=5‚10‚15‚20-tetra (2,3,6,7-tetrahydro-1H,5H-benzo [ij] puinolizine) porphyrin),Si (TMP)Cl2(X=5,10,15,20-tetra (N,N-dimethylphenyl) porphyrin) have been investigated using density functional theory (DFT) to assess the influence of ruffled conformation on the electronic structures, frontier molecular orbital, charge carrier transport, electronic spectra. The electronic structures reveal that all these Si porphyrins display visible ruffling distortion, as the dihedral angle Cα2-N2-N4-Cα4 are ca. 30 ̊. And calculations confirm that ruffed distortion result in higher LUMO energies, lower EA values than corresponding planed Zn porphyrins, especial for similar λhole and λelectron values. These calculations suggest that the ruffled conformation bring about better charge injection and transport, which would broaden the application of distorted porphyrin in several different fields.

APA, Harvard, Vancouver, ISO, and other styles

48

Mancini, Raffaella, Enza Piccolo, Stefania Mariggio', Beatrice Maria Filippi, Cristiano Iurisci, Paolo Pertile, Christopher P. Berrie, and Daniela Corda. "Reorganization of Actin Cytoskeleton by the Phosphoinositide Metabolite Glycerophosphoinositol 4-Phosphate." Molecular Biology of the Cell 14, no. 2 (February 2003): 503–15. http://dx.doi.org/10.1091/mbc.e02-04-0179.

Full text

Abstract:

Glycerophosphoinositol 4-phosphate (GroPIns-4P) is a biologically active, water-soluble phospholipase A metabolite derived from phosphatidylinositol 4-phosphate, whose cellular concentrations have been reported to increase in Ras-transformed cells. It is therefore important to understand its biological activities. Herein, we have examined whether GroPIns-4P can regulate the organization of the actin cytoskeleton, because this could be a Ras-related function involved in cell motility and metastatic invasion. We find that in serum-starved Swiss 3T3 cells, exogenously added GroPIns-4P rapidly and potently induces the formation of membrane ruffles, and, later, the formation of stress fibers. These actin structures can be regulated by the small GTPases Cdc42, Rac, and Rho. To analyze the mechanism of action of GroPIns-4P, we selectively inactivated each of these GTPases. GroPIns-4P requires active Rac and Rho, but not Cdc42, for ruffle and stress fiber formation, respectively. Moreover, GroPIns-4P induces a rapid translocation of the green fluorescent protein-tagged Rac into ruffles, and increases the fraction of GTP-bound Rac, in intact cells. The activation of Rac by GroPIns-4P was near maximal and long-lasting. Interestingly, this feature seems to be critical in the induction of actin ruffles by GroPIns-4P.

APA, Harvard, Vancouver, ISO, and other styles

49

Myrdal, S. E., and N. Auersperg. "An agent or agents produced by virus-transformed cells cause unregulated ruffling in untransformed cells." Journal of Cell Biology 102, no. 4 (April 1, 1986): 1224–29. http://dx.doi.org/10.1083/jcb.102.4.1224.

Full text

Abstract:

KNRK cells (a normal rat kidney [NRK] cell line transformed by Kirsten murine sarcoma virus) in sparse culture exhibit a highly ruffled morphology, but the cause of this ruffling is unknown. In this study, we have demonstrated that the continuous, excess ruffling on KNRK cells is caused by one or more soluble agents secreted by the KNRK cells themselves. To do this study, an assay for ruffling responses in live cell cultures was defined, and its reproducibility was demonstrated. This assay permitted observation of the kinetics of ruffling responses (percentage of cells ruffled as a function of time after stimulation). This method was used to compare the kinetics of ruffling induced by insulin, epidermal growth factor, fibroblast growth factor, glucose, and KNRK cell conditioned medium (CM). Ruffling was elicited on NRK cells by each of the polypeptide mitogens and nutrients, but, in each case, this ruffling subsided spontaneously within an hour. CM from KNRK cells also caused ruffling movements on untransformed NRK cells, but this ruffling continued for at least 20 h. This response was largely blocked by premixing the KNRK cell CM with rabbit IgG against rat transforming growth factor, type alpha, (TGF-alpha). KNRK cells made quiescent (ruffle free) by a pH shift (from 7.4 to 8.4) responded to insulin, glucose, and KNRK cell CM with kinetics similar to those observed for each of these factors in NRK cells. The unusual feature for the ruffle-inducing agent(s) produced by KNRK cells was that this activity was not subject, in either NRK or KNRK cells, to the cellular off-regulation that limits the responses to insulin or glucose. Thus, the continuous ruffling of KNRK cells is caused by their own unregulated ruffle-inducing agent or agents, which appear to include TGF-alpha. This work also demonstrates that kinetic analysis of cellular responses to exogenous factors can provide new insights into the regulatory mechanisms involved in the normal limitation of these responses.

APA, Harvard, Vancouver, ISO, and other styles

50

Ruusala, A., C. Sundberg, A. K. Arvidsson, E. Rupp-Thuresson, C. H. Heldin, and L. Claesson-Welsh. "Platelet-derived growth factor (PDGF)-induced actin rearrangement is deregulated in cells expressing a mutant Y778F PDGF beta-receptor." Journal of Cell Science 111, no. 1 (January 1, 1998): 111–20. http://dx.doi.org/10.1242/jcs.111.1.111.

Full text

Abstract:

Platelet-derived growth factor-stimulated actin rearrangement and edge ruffle formation have previously been shown to be dependent on activation of phosphatidylinositol 3′-kinase, the activity of which also is important for directed migration of cells. This lipid kinase binds to phosphorylated tyrosine residues Y740 and Y751 in the kinase insert of the human platelet-derived growth factor ss-receptor. We examined the role of two other tyrosine residues in the kinase insert of this receptor, Y775 and Y778, for ligand-induced actin rearrangement. Both were shown to be phosphorylation sites; Y775 was only marginally phosphorylated in cells expressing the wild-type ss-receptor, whereas Y778 was phosphorylated at higher stoichiometry. Mutant receptors Y775F, Y778F and Y775/778F were active kinases and mediated proliferative responses when expressed in porcine aortic endothelial cells. Fluorescence staining of actin in platelet-derived growth factor-stimulated PAE cells revealed that Y778 is involved in regulation of the actin cytoskeleton since the cells contained, apart from edge ruffles and circular ruffles, a novel type of giant ruffle on the dorsal side of the cell, which consisted of irregular multilayered actin structures. Mutation at Y778 had no effect on activation of phosphatidylinositol 3′-kinase, nor on the GTPase activating protein of Ras and phospholipase C(gamma), and the extent of directed migration towards platelet-derived growth factor of these cells was not changed. We conclude that actin rearrangement is regulated in part by Y778 in the platelet-derived growth factor ss-receptor, potentially through binding of a novel signaling molecule to this site.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Ruffier'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles