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Journal articles on the topic 'Rumen fermentation'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Purcell, Peter James, Tommy M. Boland, Martin O'Brien, and Pádraig O'Kiely. "In vitro rumen methane output of forb species sampled in spring and summer." Agricultural and Food Science 21, no. 2 (2012): 83–90. http://dx.doi.org/10.23986/afsci.4811.

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The chemical composition, in vitro rumen fermentation variables and methane (CH4) output of a range of common forb species sampled in spring and summer, and grass silage (14 treatments in total), were determined in this study. Dried, milled herbage samples were incubated in an in vitro rumen batch culture with rumen microbial inoculum (rumen fluid) and buffered mineral solution (artificial saliva) at 39 °C for 24 hours. All herbage chemical composition and in vitro rumen fermentation variables were affected (p<0.001) by treatment. Rumex obtusifolius (in spring and summer), Urtica dioica (su

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NAGARAJA, T. G., S. J. GALITZER, D. L. HARMON, and S. M. DENNIS. "EFFECT OF LASALOCID, MONENSIN AND THIOPEPTIN ON LACTATE PRODUCTION FROM IN VITRO RUMEN FERMENTATION OF STARCH." Canadian Journal of Animal Science 66, no. 1 (1986): 129–39. http://dx.doi.org/10.4141/cjas86-014.

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Starch fermentations with strained rumen fluid and centrifuged rumen fluid devoid of protozoa were set up to test the effect of lasalocid, monensin, and thiopeptin on L(+) and D(−) lactate production. Protozoa-free rumen fluid was the supernatant from low-speed centrifugation of strained rumen fluid. Starch fermentation in the control (no antibiotic) with centrifuged rumen fluid resulted in higher lactate concentration than the fermentation with strained rumen fluid. Decreased lactate production with strained rumen fluid was attributed to sequestration of starch by protozoa and to enhanced lac

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3

Arya, Anjali, PM Lunagariya, RJ Modi, and YG Patel. "Rumen fermentation." International Journal of Veterinary Sciences and Animal Husbandry 9, no. 5 (2024): 09–12. http://dx.doi.org/10.22271/veterinary.2024.v9.i5a.1639.

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Moningkey, Sony A. E., R. A. V. Tuturoong, and I. D. R. Lumenta. "PEMANFAATAN ISI RUMEN TERFERMENTASI CELLULOMONAS Sp SEBAGAI CAMPURAN PAKAN KOMPLIT TERNAK KELINCI." ZOOTEC 40, no. 1 (2020): 352. http://dx.doi.org/10.35792/zot.40.1.2020.28245.

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UTILIZATION OF FERMENTED RUMENT CONTENT WITH CELLULOMONAS SP IN MIXED COMPLETE FEED FOR RABBIT. Research conducted to learn how to use cattle rumen content by using fermentation processing techniques to enable this rumen to be used as rabbit feed. The material used in this study consisted of cattle rumen contents, starter Cellulomonas sp, rabbits, complete feed. This research consisted of two phase. The first study used an experimental method with a completely randomized design 4 preparations and 6 replications. The fermentation time consists of 0 hours, 24 hours, 48 hours and 72 hours. For th

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Jalč, D., and M. Čertík. "Effect of microbial oil, monensin and fumarate on rumen fermentation in artificial rumen." Czech Journal of Animal Science 50, No. 10 (2011): 467–72. http://dx.doi.org/10.17221/4238-cjas.

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The objective of this study was to investigate the effect of microbial oil on rumen fermentation of a diet composed of 60% hay and 40% barley in an artificial rumen (Rusitec). Microbial oil (MO) was produced by the fungus Thamnidium elegans. This fungus grew on the wheat bran/spent malt grains (3:1) mixture. The fatty acid composition of microbial oil was as follows: 0.7% C<sub>14:0</sub>, 15.4% C<sub>16:0</sub>, 10.1% C<sub>18:0</sub>, 50.9% C<sub>18:1</sub>, 13.9% C<sub>18:2</sub> and 8.4% C<sub>18:3</sub> (GLA, &gam

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Banik, B. K., Z. Durmic, W. Erskine, K. Ghamkhar, and C. Revell. "In vitro ruminal fermentation characteristics and methane production differ in selected key pasture species in Australia." Crop and Pasture Science 64, no. 9 (2013): 935. http://dx.doi.org/10.1071/cp13149.

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Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermenta

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Rarumangkay, Jeni. "PENGARUH FERMENTASI ISI RUMEN SAPI DENGAN Trichoderma viride TERHADAP ENERGI METABOLIS PADA AYAM BROILER." ZOOTEC 35, no. 2 (2015): 312. http://dx.doi.org/10.35792/zot.35.2.2015.8569.

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THE EFFECT OF DRIED COW RUMEN FERMENTATION WITH TRICHODERMA VIRIDE ON METABOLIZABLE ENERGY VALUE OF BROILER. The purpose of this experiment was to determine the metabolizable energy of dried cow rumen. The experiment use dried cow rumen and dried cow rumen fermented Trichoderma viride during 9 days with 0,3% inoculum dose. The experiment use 18 six weeks old male broiler metabolizable energy parameter were analyzed with Wilcoxon test. The result of this experiment showed fermentation with Trichoderma viride could increase the metabolizable energy of dried cow rumen. Key word : Fermentation of

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Nagadi, S., M. Herrero, and N. S. Jessop. "Effect of frequency of ovine ruminal sampling on microbial activity and substrate fermentation." Proceedings of the British Society of Animal Science 1999 (1999): 154. http://dx.doi.org/10.1017/s1752756200003094.

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Food eaten by a ruminant firstly undergoes microbial fermentation within the rumen. Nutritionally important characteristics of the food are the rate and extent of fermentation of its carbohydrate fraction, which can both be estimated using the in vitro gas production technique. The single greatest source of uncontrolled variation in any in vitro rumen fermentation system is the rumen fluid; curves produced from gas production data were influenced significantly by the variation in microbial activity between days (Menke and Steingass, 1988; Beuvink et al, 1992). A more reliable measure of rumen

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9

Walker, Charles E., James S. Drouillard, and Tiruvoor G. Nagaraja. "Optaflexx1 affects rumen fermentation." Kansas Agricultural Experiment Station Research Reports, no. 1 (January 1, 2007): 88–90. http://dx.doi.org/10.4148/2378-5977.1536.

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10

Castillo-González, AR, ME Burrola-Barraza, J. Domínguez-Viveros, and A. Chávez-Martínez. "Rumen microorganisms and fermentation." Archivos de medicina veterinaria 46, no. 3 (2014): 349–61. http://dx.doi.org/10.4067/s0301-732x2014000300003.

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Bagheri, M., G. R. Ghorbani, H. R. Rahmani, and M. Khorvash. "Effect of yeast and mannan-oligosaccharides on in vitro fermentation of different substrates." Proceedings of the British Society of Animal Science 2009 (April 2009): 91. http://dx.doi.org/10.1017/s1752756200029306.

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Results of yeast effects on in vitro rumen fermentation are inconsistent (Sullivan et al., 1999; Yang et al., 2004). In addition, there is no data on the effect of mannan-oligosacchrides (MOS) and their interaction with yeast on rumen fermentation. This trial was conducted to study the effects of yeast and MOS on rumen fermentation of different substrates.

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Wilk, Martyna, Ewa Pecka-Kiełb, Jerzy Pastuszak, Muhammad Umair Asghar, and Laura Mól. "Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production." Agriculture 12, no. 11 (2022): 1943. http://dx.doi.org/10.3390/agriculture12111943.

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Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different types of copper supplement, their rumen decomposition and effect on in vitro ruminal fermentation as well as methanogenesis. Two different copper additives were used in the experiment: CS—copper sulfate (CuSO4 × 5 H2O)—and EC—encapsulated copper (tribasic copper chloride and copper sulfate enclosed within a polysaccharide polymer coating). A total mixed rati

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Newbold, C. J., R. J. Wallace, and I. M. Nevison. "Influence of ionophores on in vitro fermentation by rumen fluid from sheep receiving yeast culture (Yeasacc; YC)." Proceedings of the British Society of Animal Production (1972) 1991 (March 1991): 78. http://dx.doi.org/10.1017/s0308229600020286.

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A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the ru

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Candyrine, S. C. L., M. F. Jahromi, M. Ebrahimi, J. B. Liang, Y. M. Goh, and N. Abdullah. "In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids." Animal Production Science 57, no. 8 (2017): 1607. http://dx.doi.org/10.1071/an15684.

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An in vitro gas-production study was conducted to compare differences in rumen fermentation characteristics and the effect of supplementation of 4% linseed oil as a source of polyunsaturated fatty acids on the rumen fermentation profile in rumen fluid collected from goats and sheep. Rumen fluid for each species was obtained from two male goats of ~18 months old and two sheep of similar sex and age fed the similar diet containing 30% alfalfa hay and 70% concentrates. The substrate used for the fermentation was alfalfa hay and concentrate mixture (30:70) without (control) and with addition of li

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Li, Jinhui, Hui Yan, Jiaxin Chen, et al. "Correlation of Ruminal Fermentation Parameters and Rumen Bacterial Community by Comparing Those of the Goat, Sheep, and Cow In Vitro." Fermentation 8, no. 9 (2022): 427. http://dx.doi.org/10.3390/fermentation8090427.

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In this study, we aimed to establish the correlation between ruminal fermentation parameters and the bacterial community by comparing those of the goat, sheep, and cow, thus illustrating the main bacteria causing the difference in rumen fermentation among goats, sheep, and cows and providing a new idea for improving the feed digestibility of ruminants. Rumen fluid from goats (Taihang White cashmere goat, n = 6), sheep (Hu sheep, n = 6), and cows (Holstein cow, n = 6) was collected using oral intubation and immediately brought back to the laboratory for a fermentation test with the same total m

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Wang, Liyan, Shoukun Ji, Hui Yan, et al. "Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH4 Emission and Skatole Content In Vitro." Fermentation 9, no. 5 (2023): 428. http://dx.doi.org/10.3390/fermentation9050428.

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The purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH4) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula and immediately transferred to the laboratory for rumen fermentation in vitro. The experimental diet was supplemented with 0%, 0.2%, 0.8%, 1.2%, 1.8% and 2.4% FOS in the basal diet. Gas production (GP) and CH4 production were measured and recorded at 2, 4, 6, 8, 10, 12, 24, 36 and 48 h. After 48 h of fermentation, degradation rates of nutritional co

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Ungerfeld, Emilio M., M. Fernanda Aedo, Emilio D. Martínez, and Marcelo Saldivia. "Inhibiting Methanogenesis in Rumen Batch Cultures Did Not Increase the Recovery of Metabolic Hydrogen in Microbial Amino Acids." Microorganisms 7, no. 5 (2019): 115. http://dx.doi.org/10.3390/microorganisms7050115.

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There is an interest in controlling rumen methanogenesis as an opportunity to both decrease the emissions of greenhouse gases and improve the energy efficiency of rumen fermentation. However, the effects of inhibiting rumen methanogenesis on fermentation are incompletely understood even in in vitro rumen cultures, as the recovery of metabolic hydrogen ([H]) in the main fermentation products consistently decreases with methanogenesis inhibition, evidencing the existence of unaccounted [H] sinks. We hypothesized that inhibiting methanogenesis in rumen batch cultures would redirect [H] towards mi

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Dewi, Wuryani Kusuma, Limbang Kustiawan Nuswantara, and Surono Surono. "Digestibility of Coconut Coir Fiber Fermented by Buffalo Rumen Fluid Microbes in Vitro." Jurnal Agripet 25, no. 1 (2025): 95–100. https://doi.org/10.17969/agripet.v25i1.30112.

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This study aimed to examine the digestibility of fiber, including crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility of coconut husk fermented using selected buffalo rumen microbes with varying percentages of inoculum and fermentation periods. The study utilized a completely randomized design (CRD) with a factorial pattern (3×3) and 3 replications. The first factor was the percentage of inoculum (0%, 2.5%, and 5% mL of inoculum per gram of dry matter of coconut husk), and the second factor was the fermentation period (0, 7, and 14 days). The collected data

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Hesni, V., A. Taghizadeh, H. Paya, H. Janmohamadi, G. A. Moghadam, and N. Pirani. "Effect of monensin and lasalocid on rumen fermentation in sheep." Proceedings of the British Society of Animal Science 2007 (April 2007): 221. http://dx.doi.org/10.1017/s1752756200021244.

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Ionophores, consisting of monensin and lasalocid were reported to improve rumen fermentation and metabolism of ruminants when delivered in their respective proper dosage (Mass et al., 2001). However, the efficacy of ionophores in the manipulation of rumen fermentation has been shown to vary with diet. Ionophores may also inhibit ruminal amino acid deamination (Yang et al., 2003). The objective of this study was to determine of the effect of monensin and lasalocid on rumen fermentation characteristics.

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YANG, H. J., H. ZHUANG, X. K. MENG, D. F. ZHANG, and B. H. CAO. "Effect of melamine onin vitrorumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures." Journal of Agricultural Science 152, no. 4 (2013): 686–96. http://dx.doi.org/10.1017/s0021859613000725.

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SUMMARYThe effects of melamine on gas production (GP) kinetics, methane (CH4) production and fermentation of diets differing in forage content (low-forage (LF) diet: 200 g/kg and high-forage (HF) diet: 800 g/kg) by rumen micro-organismsin vitrowere studied using batch cultures. Rumen contents were collected from three Simmental×Luxi crossbred beef cattle. Melamine was added to the incubation bottles to achieve final concentration of 0 (control), 2, 6, 18, 54, 162 and 484 mg/kg of each diet. Cumulative GP was continuously measured in an automated gas recording instrument during 72 h of incubati

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Wang, Xinjie, Jianzhao Zhou, Runjie Jiang, et al. "Development of an Alternative In Vitro Rumen Fermentation Prediction Model." Animals 14, no. 2 (2024): 289. http://dx.doi.org/10.3390/ani14020289.

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The aim of this study is to identify an alternative approach for simulating the in vitro fermentation and quantifying the production of rumen methane and rumen acetic acid during the rumen fermentation process with different total mixed rations. In this experiment, dietary nutrient compositions (neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and dry matter (DM)) were selected as input parameters to establish three prediction models for rumen fermentation parameters (methane and acetic acid): an artificial neural network model, a genetic algorithm-bp model, and a

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Yu, Jiangkun, Liyuan Cai, Jiacai Zhang, et al. "Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro." Microorganisms 8, no. 8 (2020): 1160. http://dx.doi.org/10.3390/microorganisms8081160.

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This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane product

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Vigh, Antal, Adriana Criste, Kévin Gragnic, Léa Moquet, and Christine Gerard. "Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro." Agriculture 13, no. 4 (2023): 879. http://dx.doi.org/10.3390/agriculture13040879.

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The aim of this study was to assess the effects of supplementation with inorganic sources of manganese (MnO, MnSO4), zinc (ZnO, ZnSO4) and copper (CuSO4) at different levels (0.06%DM for Mn, 0.05%DM for Zn; 0.01 and 0.05%DM for Cu) on in vitro rumen fermentation, solubility and bioavailability. Fermentation activity was measured by total gas production (TGP) and dry matter degradability after 70 h of fermentation (dDM%). Trace mineral (TM) solubility was estimated via the TM concentration in the supernatant of the final fermentation medium (SOL) and TM bioavailability from the TM concentration

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Qiu, Xinjun, Xiaoli Qin, Liming Chen, et al. "Serum Biochemical Parameters, Rumen Fermentation, and Rumen Bacterial Communities Are Partly Driven by the Breed and Sex of Cattle When Fed High-Grain Diet." Microorganisms 10, no. 2 (2022): 323. http://dx.doi.org/10.3390/microorganisms10020323.

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Hybridization in bovines is practiced with the main aim of improving production performance, which may imply the microbial variations in the rumen from the parental breed cross to their progeny. Besides, the interactions of offspring breed with sex in terms of rumen bacteria are not clear. This study aims to evaluate the variations in rumen bacterial communities in different breeds and sexes, and the correlations among fattening performance, serum biochemical parameters, and rumen fermentation. Forty-two 19.2 ± 0.67-month-old beef cattle (390 ± 95 kg of initial body weight) comprising two gene

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Huang, Jianzhi, Yueyun Sheng, Pengfei Xue, et al. "Patterns of Spatial Variation in Rumen Microbiology, Histomorphology, and Fermentation Parameters in Tarim wapiti (Cervus elaphus yarkandensis)." Microorganisms 12, no. 1 (2024): 216. http://dx.doi.org/10.3390/microorganisms12010216.

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The rumen is divided into multiple rumen sacs based on anatomical structure, and each has its unique physiological environment. Tarim wapiti preserved roughage tolerance after domestication, and adaptation to the desertified environment led to the development of a unique rumen shape and intraruminal environment. In this work, six Tarim wapiti were chosen and tested for fermentation parameters, microbes, and histomorphology in four rumen areas (Dorsal sac, DS; Ventral sac, VS; Caudodorsal blind sac, CDBS; Caudoventral blind sac, CVBS). Tarim wapiti’s rumen blind sac had better developed rumen h

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Cone, J. W., and M. A. M. Rodrigues. "Protein fermentation characteristics in rumen fluid determined with the gas production technique." Proceedings of the British Society of Animal Science 2009 (April 2009): 192. http://dx.doi.org/10.1017/s1752756200030313.

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The gas production technique was developed to determine the fermentation kinetics of organic matter in rumen fluid. However, the gas production technique can be adapted for the determination of protein fermentation characteristics. To do that the buffer must be N-free. All the N coming with the rumen fluid must be incorporated into microbial mass. This can be done by supplying the buffered rumen fluid with an excess of fast fermentable carbohydrates. To prevent a too high input of N from the rumen fluid the rumen fluid can be diluted further compared to the standard 3 times (Cone et al., 1996)

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Rinttilä, Teemu, Colm A. Moran, and Juha Apajalahti. "DHA-Rich Aurantiochytrium Biomass, a Novel Dietary Supplement, Resists Degradation by Rumen Microbiota without Disrupting Microbial Activity." Applied Microbiology 2, no. 1 (2022): 53–72. http://dx.doi.org/10.3390/applmicrobiol2010004.

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We first sought to evaluate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA), on rumen fermentation and the resistance of DHA to degradation and biohydrogenation by rumen microbes through ex vivo fermentation experiments. Subsequently, we sought to quantify the diet-derived DHA content of milk and the impact of AURA on microbial composition and metabolism in a pilot feeding trial with rumen-cannulated dairy cows. To achieve our aims, rumen fluid from cannulated cows was used as inoculum, and the effect of AURA inclusio

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Munyiva, Brenda, and Wahu Oyaya. "Effect of Rumen Fluid Dosage and Fermentation Time on Dissolved Protein Levels of Vegetable Waste Silage for Vannamei Shrimp Feed." International Journal Papier Advance and Scientific Review 2, no. 2 (2021): 20–24. http://dx.doi.org/10.47667/ijpasr.v2i2.110.

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Specifically, the goal of this research was to evaluate the dissolved protein content of vegetable waste generated during the incubation of rumen fluid for use in (Whiteleg) vannamei shrimp diet. The results of the analysis of the degree of protein hydrolysis of vegetable waste treated with the addition of rumen fluid enzymes and different fermentation times of rumen fluid revealed that the addition of rumen fluid enzymes and different fermentation times of rumen fluid had a statistically significant effect (p0.05) on the degree of protein hydrolysis of vegetable waste. But there was no signif

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Guo, Wei, Jolet K. van Niekerk, Mi Zhou, and Michael A. Steele. "PSIX-32 Assessment of Mucosa-associated Microbiota in the Colon and Rumen of Dairy Calves Fed High Plane of Milk and during Weaning Transition." Journal of Animal Science 98, Supplement_4 (2020): 311. http://dx.doi.org/10.1093/jas/skaa278.554.

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Abstract The nature of weaning is considered as the most stressful period experienced by dairy calves and has been known to negatively affect calf growth and intake. The objectives of this study were to investigate the shift of rumen and colon mucosa-associated microbiota in dairy calves fed with a high plane of milk pre- and post-weaning, and whether such shifts are associated with rumen fermentation and growth performance. Six Holstein dairy bull calves were ruminally cannulated at the second week of life followed by weaning at the end of week 6. Ruminal and colon tissue samples were biopsie

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Calsamiglia, S., P. Cardozo, A. Ferret, and A. Bach. "Changes in rumen microbial fermentation during acidosis are due to a combined effects of fermentation substrate and pH." Proceedings of the British Society of Animal Science 2007 (April 2007): 21. http://dx.doi.org/10.1017/s1752756200019244.

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The supply of high-concentrate diets results in the reduction in ruminal pH and the development of acidosis. Because the reduction of pH occurs at the same time as the diet is changed, the effects are confounded. For example, high-concentrate diets ferment towards propionate, and low rumen pH also results in greater propionate production. Which factor is responsible for the increase in rumen propionate? The objective of this study was to determine the effects of rumen pH and the type of diet (D) on rumen microbial fermentation, with the aim of developing simple mathematical model to describe t

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Guo, Yanxia, Faiz-ul Hassan, Mengwei Li, et al. "Effect of Sodium Nitrate and Cysteamine on In Vitro Ruminal Fermentation, Amino Acid Metabolism and Microbiota in Buffalo." Microorganisms 10, no. 10 (2022): 2038. http://dx.doi.org/10.3390/microorganisms10102038.

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Nitrate is used as a methane inhibitor while cysteamine is considered as a growth promoter in ruminants. The present study evaluated the effect of sodium nitrate and cysteamine on methane (CH4) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. Four treatments containing a 0.5 g of substrate were supplemented with 1 mg/mL sodium nitrate (SN), 100 ppm cysteamine hydrochloride (CS), and a combination of SN 1 mg/mL and CS 100 ppm (CS+SN), and a control (no additive) were applied in a completely randomized design. Each treatment group had five r

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Qian, Wenxi, ZhiPeng Li, Weiping Ao, Guangyong Zhao, Guangyu Li, and JianPing Wu. "Bacterial community composition and fermentation in the rumen of Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries)." Canadian Journal of Microbiology 63, no. 5 (2017): 375–83. http://dx.doi.org/10.1139/cjm-2016-0596.

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The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rumen fermentation of these animals are poorly understood. Here, we apply high-throughput sequencing to examine the bacterial community in the rumen of cattle, red deer, and sheep and measured rumen fermentation products. Overall, 548 218 high-quality sequences were obtained and then

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Durix, Andrée, C. Jean-Blain, H. P. Sallmann, and J. P. Jouany. "Use of a semicontinuous culture system (RUSITEC) to study the metabolism of ethanol in the rumen and its effects on ruminal digestion." Canadian Journal of Animal Science 71, no. 1 (1991): 115–23. http://dx.doi.org/10.4141/cjas91-013.

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The metabolism of ethanol by rumen micro-organisms and its effects on rumen fermentations have been studied in vitro in a semicontinuous fermentor (RUSITEC). Ethanol introduced in the fermentor at 1, 4 or 8 g d−1 L−1 of rumen juice induced important qualitative and quantitative modifications in the fermentation outputs. Total VFA production was increased from 5 to 40% according to the diet and ethanol concentration. Relative proportions of VFA were modified: caproate concentration increased three times, propionate and isovalerate concentrations decreased significantly in most cases. Methane pr

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Ismartoyo, Ismartoyo, Rohmiyatul Islamiyati, and Muhammad Rusdy. "Rumen Fermentation of Local Grasses Feed to Native Goat." Hasanuddin Journal of Animal Science (HAJAS) 5, no. 1 (2024): 28–35. http://dx.doi.org/10.20956/hajas.v5i1.24777.

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The aim of this research was to examine the feed rumen fermentation in the rumen of goat fed 4 different diets. This study was designed based on the Latin Square Design which consists of 4 diet treatments with 4 replications in each diet treatment. A total of 4 male goats, with relatively the same weight and age, were randomly assigned to an individual metabolic cage fed with 4 diets. The four diets studied were R1: Elephant grass, R2: Mini elephant grass, R3: Panicum maximum grass and R4: Brachiaria decumbens grass. Each diet was added with 20% of rice bran. The result of this experiment indi

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Ouda, J. O., C. J. Newbold, S. Lopez, et al. "The effect of acrylate and fumarate on fermentation and methane production in the rumen simulating fermentor (Rusitec)." Proceedings of the British Society of Animal Science 1999 (1999): 37. http://dx.doi.org/10.1017/s1752756200001927.

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Methane formation in the rumen represents a substantial loss of energy to the animal and is also a major source of greenhouse gas emissions from agriculture. Methanogenesis is the main means of disposal of hydrogen during rumen fermentation. The formation of propionate represents an alternative route of hydrogen disposal, providing sufficient propionate precursors are available. Theoretically, adding propionate precursors should stimulate propionate production and decrease methane production. In the present experiment, the effects of two potential precursors of propionate, fumarate and acrylat

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Dhakal, Rajan, André Luis Alves Neves, Rumakanta Sapkota, Prabhat Khanal, and Hanne Helene Hansen. "Prokaryote Composition and Structure of Rumen Fluid before and after In Vitro Rumen Fermentation." Fermentation 10, no. 2 (2024): 108. http://dx.doi.org/10.3390/fermentation10020108.

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Background: This study aimed to investigate the impact of in vitro rumen fermentation (IVRF) on the microbiome structure and composition of rumen fluid before and after fermentation assays. Methods and Results: Six separate fermentation batches were run for 48 h using maize silage as the basal feed. Rumen fluid samples were analyzed before (RF; only rumen fluid inoculant) and after 48 h fermentation assay (MS; maize silage as the substrate) and further processed for microbiome analysis using amplicon sequencing targeting the V4 region of the bacterial 16S rRNA gene. Bacterial alpha diversity r

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TKALCIC, SUZANA, CATHY A. BROWN, BARRY G. HARMON, et al. "Effects of Diet on Rumen Proliferation and Fecal Shedding of Escherichia coli O157:H7 in Calves." Journal of Food Protection 63, no. 12 (2000): 1630–36. http://dx.doi.org/10.4315/0362-028x-63.12.1630.

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Calves inoculated with Escherichia coli O157:H7 and fed either a high-roughage or high-concentrate diet were evaluated for rumen proliferation and fecal shedding of E. coli O157:H7. Calves fed the high-roughage diet had lower mean rumen volatile fatty acid concentrations and higher rumen pH values than did calves fed the high-concentrate diet. Despite these differences in rumen conditions, the calves fed the high-roughage diet did not have greater rumen populations of E. coli O157: H7 and did not exhibit increased or longer fecal shedding compared with the calves fed the high-concentrate diet.

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Fakhri, S., A. R. Moss, D. I. Givens, and E. Owen. "Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds." Proceedings of the British Society of Animal Science 1997 (1997): 196. http://dx.doi.org/10.1017/s1752756200596379.

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Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.

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Hussain, A., and E. L. Miller. "Effect of supplementation of sucrose and lactose with sodium bicarbonate on rumen metabolism and microbial protein synthesis in sheep." Proceedings of the British Society of Animal Science 1999 (1999): 28. http://dx.doi.org/10.1017/s1752756200001836.

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Sucrose and lactose are used as energy supplements in ruminant diets. In our previous study (Hussain and Miller, 1998) lactose maintained a higher rumen pH, increased organic matter and neutral detergent fibre digestion in the rumen, reduced the number of rumen protozoa and increased microbial (bacterial) protein flow to the duodenum compared with sucrose. However, it was not clear whether the effect of lactose on pH or on reduction of protozoa was the main factor increasing rumen fermentation and microbial protein flow. The objective of the present study was to examine the effect of isoenerge

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Astuti, W. D., Y. Widyastuti, E. Wina, S. Suharti, R. Ridwan, and K. G. Wiryawan. "Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR." Journal of the Indonesian Tropical Animal Agriculture 43, no. 2 (2018): 184. http://dx.doi.org/10.14710/jitaa.43.2.184-192.

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The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentatio

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CIESLAK, A., P. ZMORA, A. STOCHMAL, et al. "Rumen antimethanogenic effect ofSaponaria officinalisL. phytochemicalsin vitro." Journal of Agricultural Science 152, no. 6 (2014): 981–93. http://dx.doi.org/10.1017/s0021859614000239.

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SUMMARYAlthough the effect of saponins or saponin-containing plants on rumen microorganisms and rumen fermentation has been intensively investigated, this issue still requires special attention. Many of the phenomena occurring in the rumen related to dietary saponin supplementation are still not fully understood.Saponaria officinalisis a triterpenoid saponin-containing plant; thus, the aim of the present study was to evaluate the effect ofS. officinalisL. powdered root, methanolic extract of theS. officinalisroot (SOR) and the effect of the separated fractions (polysaccharides, saponins and ph

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Wei, Xiao, Kehui Ouyang, Tanghui Long, Zuogui Liu, Yanjiao Li, and Qinghua Qiu. "Dynamic Variations in Rumen Fermentation Characteristics and Bacterial Community Composition during In Vitro Fermentation." Fermentation 8, no. 6 (2022): 276. http://dx.doi.org/10.3390/fermentation8060276.

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This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), fermentation at 12 h (R12, n = 10), and fermentation at 24 h (R24, n = 10). Results showed that gas production, concentrations of microbial crude protein, ammonia nitrogen, and individual volatile fatty acids (VFA), as well as total VFA and branched-chain VFA concentrations, were higher in R24 when compared with R12 (p < 0.05). However, no signif

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Wu, Yinglian, Chong Jiao, Qiyu Diao, and Yan Tu. "Effect of Dietary and Age Changes on Ruminal Microbial Diversity in Holstein Calves." Microorganisms 12, no. 1 (2023): 12. http://dx.doi.org/10.3390/microorganisms12010012.

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Ruminal microorganisms play a crucial role in the energy supply of ruminants and animal performance. We analyzed the variations in rumen bacteria and fungi at 45 d, 75 d, and 105 d by using 16SrRNA and ITS sequencing data and investigated their correlation with rumen fermentation. According to the results, rumen microflora tended to gradually mature with age, and bacterial and fungal establishment gradually stabilized. Upon comparing the three periods, the concentration of propionic acid increased significantly (p < 0.05) after weaning, and weaning accompanied by a transition in diet remark

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Li, Qin, Yan Tu, Tao Ma, et al. "Effects of Two Feeding Patterns on Growth Performance, Rumen Fermentation Parameters, and Bacterial Community Composition in Yak Calves." Microorganisms 11, no. 3 (2023): 576. http://dx.doi.org/10.3390/microorganisms11030576.

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The health of young ruminants is highly dependent on early rumen microbial colonization. In this study, the effects of milk replacer on growth performance, rumen fermentation, and the rumen microflora in yak calves were evaluated. Sixty yak calves (body weight = 22.5 ± 0.95 kg, age = 30 ± 1 d) were assigned to the CON group (breastfed) or TRT group (milk replacer fed) and evaluated over 120 d. At 120 d, ruminal fluid samples were collected from 14 calves and then conducted for rumen fermentation and microbiota analyses. There was no difference in growth performance; however, calf survival was

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Newbold, C. J., and R. J. Wallace. "The effect of yeast and distillery by-products on the fermentation in the rumen simulation technique (rusitec)." Proceedings of the British Society of Animal Production (1972) 1992 (March 1992): 210. http://dx.doi.org/10.1017/s0308229600023199.

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The practice of adding low levels of non-commensal yeast and fungi to ruminant diets is increasingly gaining acceptance as a means of manipulating rumen fermentation to benefit production. Reported benefits include an increased degradability of forages in the rumen and an improved flow of microbial protein from the rumen (Williams and Newbold, 1990).Distillery by-products, such as pot ale syrup, are commonly included in ruminant diets as an energy source. However, pot ale syrup contains a substantial number of yeast cells. The aim of the present study was to establish if different yeasts and y

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Rabee, Alaa Emara, Khalid Z. Kewan, Hassan M. El Shaer, Mebarek Lamara, and Ebrahim A. Sabra. "Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep." AIMS Microbiology 8, no. 1 (2022): 26–41. http://dx.doi.org/10.3934/microbiol.2022003.

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<abstract> <p>Rumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (<italic>Phoenix dactylifera</italic>) and olive (<italic>Olea europaea</italic>) trees have received attention to be used in animal feeding. This study evaluated the impact of non-traditional feed resources including olive cake (OC), discarded dates (DD), and date palm frond (DPF) in sheep diet on rumen

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Kingston-Smith, Alison H., Joan E. Edwards, Sharon A. Huws, Eun J. Kim, and Michael Abberton. "Plant-based strategies towards minimising ‘livestock's long shadow’." Proceedings of the Nutrition Society 69, no. 4 (2010): 613–20. http://dx.doi.org/10.1017/s0029665110001953.

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Ruminant farming is an important component of the human food chain. Ruminants can use offtake from land unsuitable for cereal crop cultivation via interaction with the diverse microbial population in their rumens. The rumen is a continuous flow fermenter for the digestion of ligno-cellulose, with microbial protein and fermentation end-products incorporated by the animal directly or during post-ruminal digestion. However, ruminal fermentation is inefficient in capturing the nutrient resource presented, resulting in environmental pollution and generation of greenhouse gases. Methane is generated

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Nueraihemaiti, Gulinizier, Xiangdong Huo, Huiying Zhang, et al. "Effect of Diet Supplementation with Two Yeast Cultures on Rumen Fermentation Parameters and Microbiota of Fattening Sheep In Vitro." Microorganisms 13, no. 3 (2025): 550. https://doi.org/10.3390/microorganisms13030550.

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Yeast culture can improve ruminant health and reduce economic losses in intensive farming, but as a non-standardized product in China, its quality and efficacy vary significantly. In this study, a self-developed yeast culture was compared with a commercially available product using in vitro rumen fermentation and amplicon-based high-throughput sequencing to evaluate its effects on rumen fermentation parameters, microbial diversities, and community compositions in Hu sheep. The aim was to validate the efficacy and mechanisms of the self-developed yeast culture, produced with simplified raw mate

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Xue, Ligang, Shuyi Zhou, Dan Wang, Fangyu Zhang, Junfeng Li, and Liyuan Cai. "The Low Dose of Saccharomyces cerevisiae Is Beneficial for Rumen Fermentation (Both In Vivo and In Vitro) and the Growth Performance of Heat-Stressed Goats." Microorganisms 10, no. 10 (2022): 1877. http://dx.doi.org/10.3390/microorganisms10101877.

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This study aimed to investigate the effects of Saccharomyces cerevisiae on rumen fermentation and the growth performance of heat-stressed goats. The fermentation experiment was conducted using Saccharomyces cerevisiae added at 0‰ (HS1), 0.30‰ (SC1), 0.60‰ (SC2), and 1.20‰ (SC3) of the dry matter (DM) weight of the basal diet. The results showed that supplementing with 0.60‰ (SC2) could increase the pH, acetic acid to propionic acid ratio, the concentrations of ammonia nitrogen, total volatile fatty acids, acetic acid, propionic acid, butyric acid, and the degradability of DM, neutral detergent

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Mantovani, Hilario. "90 Microbiome-Derived Bioactive Molecules to Reduce Enteric Methane Emissions." Journal of Animal Science 101, Supplement_2 (2023): 234–35. http://dx.doi.org/10.1093/jas/skad341.264.

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Abstract Microorganisms are pivotal for the development, health, and productivity of livestock. In adult ruminants, the microbiota colonizing the rumen is essential for efficient depolymerization of indigestible carbohydrates from plant biomass and their conversion into microbial protein and fermentation end-products that are used by the host for growth. In the last decades, next-generation sequencing and omics technologies provided unparalleled insights into the composition, structure, and function of the gastrointestinal (GI) tract microbiome of ruminants and helped improve our understanding

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