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1
Purcell, Peter James, Tommy M. Boland, Martin O'Brien, and Pádraig O'Kiely. "In vitro rumen methane output of forb species sampled in spring and summer." Agricultural and Food Science 21, no. 2 (June 5, 2012): 83–90. http://dx.doi.org/10.23986/afsci.4811.
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The chemical composition, in vitro rumen fermentation variables and methane (CH4) output of a range of common forb species sampled in spring and summer, and grass silage (14 treatments in total), were determined in this study. Dried, milled herbage samples were incubated in an in vitro rumen batch culture with rumen microbial inoculum (rumen fluid) and buffered mineral solution (artificial saliva) at 39 °C for 24 hours. All herbage chemical composition and in vitro rumen fermentation variables were affected (p<0.001) by treatment. Rumex obtusifolius (in spring and summer), Urtica dioica (summer) and Senecio jacobaea (summer) had lower (p<0.05) CH4 outputs relative to feed dry matter incubated compared with grass silage, reflecting their lower extent of in vitro rumen fermentation.
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NAGARAJA, T. G., S. J. GALITZER, D. L. HARMON, and S. M. DENNIS. "EFFECT OF LASALOCID, MONENSIN AND THIOPEPTIN ON LACTATE PRODUCTION FROM IN VITRO RUMEN FERMENTATION OF STARCH." Canadian Journal of Animal Science 66, no. 1 (March 1, 1986): 129–39. http://dx.doi.org/10.4141/cjas86-014.
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Starch fermentations with strained rumen fluid and centrifuged rumen fluid devoid of protozoa were set up to test the effect of lasalocid, monensin, and thiopeptin on L(+) and D(−) lactate production. Protozoa-free rumen fluid was the supernatant from low-speed centrifugation of strained rumen fluid. Starch fermentation in the control (no antibiotic) with centrifuged rumen fluid resulted in higher lactate concentration than the fermentation with strained rumen fluid. Decreased lactate production with strained rumen fluid was attributed to sequestration of starch by protozoa and to enhanced lactate fermentation. Addition of lasalocid or monensin (1.5–48.0 μg mL−1) to the fermentation enhanced L(+) and D(−) lactate production in the presence of protozoa. In the absence of protozoa, lasalocid and monensin inhibited L(+) lactate production; however, D(−) lactate concentration was unaffected. Increased lactate production by lasalocid and monensin in the presence of protozoa was possibly due to inhibition of protozoal engulfment of starch. Thiopeptin had no effect on lactate production in the presence of protozoa but in the absence of protozoa lactate production was inhibited. Similar antibiotic responses were observed at different starch amounts (0.5, 1.5 and 3.0 g) and with starch types (soluble, corn and wheat) and with rumen fluid collected from defaunated cattle. Key words: Antibiotics, cattle, rumen, starch, fermentation, lactic acid
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Arya, Anjali, PM Lunagariya, RJ Modi, and YG Patel. "Rumen fermentation." International Journal of Veterinary Sciences and Animal Husbandry 9, no. 5 (January 1, 2024): 09–12. http://dx.doi.org/10.22271/veterinary.2024.v9.i5a.1639.
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Moningkey, Sony A. E., R. A. V. Tuturoong, and I. D. R. Lumenta. "PEMANFAATAN ISI RUMEN TERFERMENTASI CELLULOMONAS Sp SEBAGAI CAMPURAN PAKAN KOMPLIT TERNAK KELINCI." ZOOTEC 40, no. 1 (January 31, 2020): 352. http://dx.doi.org/10.35792/zot.40.1.2020.28245.
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UTILIZATION OF FERMENTED RUMENT CONTENT WITH CELLULOMONAS SP IN MIXED COMPLETE FEED FOR RABBIT. Research conducted to learn how to use cattle rumen content by using fermentation processing techniques to enable this rumen to be used as rabbit feed. The material used in this study consisted of cattle rumen contents, starter Cellulomonas sp, rabbits, complete feed. This research consisted of two phase. The first study used an experimental method with a completely randomized design 4 preparations and 6 replications. The fermentation time consists of 0 hours, 24 hours, 48 hours and 72 hours. For the second study using an experimental method with randomized block design based on the initial body weight of rabbits. The treatment given is the level of use of the best fermented rumen contents in a complete feed ration. Variable which is translated as feed consumption, body weight gain and feed conversion. Research results The first stage of the P4 study sample (72 hours) as the best guideline is seen from the parameters of crude protein and crude fiber. The results of this study indicate that the use of feed using rumen fermentation (IRF) can increase feed consumption and weight gain. The conclusion of this study is the provision of 30% mixture of fermented rumen contents of Cellulomonas sp in complete feed produced the best results seen from the parameters of consumption, weight gain and feed conversion of rabbit.Keywords: Rumen contents, fermentation, Cellulomonas sp, complete feed, rabbits
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Jalč, D., and M. Čertík. "Effect of microbial oil, monensin and fumarate on rumen fermentation in artificial rumen." Czech Journal of Animal Science 50, No. 10 (December 11, 2011): 467–72. http://dx.doi.org/10.17221/4238-cjas.
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The objective of this study was to investigate the effect of microbial oil on rumen fermentation of a diet composed of 60% hay and 40% barley in an artificial rumen (Rusitec). Microbial oil (MO) was produced by the fungus Thamnidium elegans. This fungus grew on the wheat bran/spent malt grains (3:1) mixture. The fatty acid composition of microbial oil was as follows: 0.7% C<sub>14:0</sub>, 15.4% C<sub>16:0</sub>, 10.1% C<sub>18:0</sub>, 50.9% C<sub>18:1</sub>, 13.9% C<sub>18:2</sub> and 8.4% C<sub>18:3</sub> (GLA, &gamma;-linolenic acid). The effect of monensin MON (66 ppm) and fumarate FUM (6.25 mmol) with and without MO supplementation was also studied. The experiment in Rusitec lasted 11 days. After a stabilization period (5 days), MO was added to fermentation vessel V<sub>2</sub> (6 days), MON to fermentation vessel V<sub>3</sub> (6 days) and FUM to fermentation vessel V<sub>4 </sub>(6 days). MO was also added to V<sub>3</sub> and V<sub>4</sub> on the last day together with MON (V<sub>3</sub>) and FUM (V<sub>4</sub>). The fermentation vessel V<sub>1 </sub>served as control (without additives). The results showed that MO reduced (P &lt; 0.05) mol% acetate and increased (P &lt; 0.05) mol% propionate and n-butyrate. Methane production (mmol/day) was reduced numerically (NS). The efficiency of microbial synthesis (EMS) was also reduced numerically and nitrogen incorporated by the microflora (N<sub>M</sub>) was reduced significantly in MO supplementation. There were no differences in the rumen fermentation when MO was applied together with MON and FUM compared to the vessel where only MO was applied. No additive effect was observed in the relationship MO-ionophore or MO-FUM. Monensin and fumarate applied separately showed their typical effects on rumen fermentation in vitro. &nbsp;
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Banik, B. K., Z. Durmic, W. Erskine, K. Ghamkhar, and C. Revell. "In vitro ruminal fermentation characteristics and methane production differ in selected key pasture species in Australia." Crop and Pasture Science 64, no. 9 (2013): 935. http://dx.doi.org/10.1071/cp13149.
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Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermentation, with the lowest methane-producing species, Biserrula pelecinus L., producing 90% less methane (4 mL CH4 g–1 dry matter incubated) than the highest methane-producing species, Trifolium spumosum L. (51 mL CH4 g–1 dry matter incubated). Proxy nutritive values of species were found not to be useful predictors of plant fermentation characteristics or methane production. In conclusion, there were significant differences in fermentative traits, including methane production, among selected pasture species in Australia, indicating that the choice of fodder species may offer a way to reduce the impact on the environment from enteric fermentation.
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Rarumangkay, Jeni. "PENGARUH FERMENTASI ISI RUMEN SAPI DENGAN Trichoderma viride TERHADAP ENERGI METABOLIS PADA AYAM BROILER." ZOOTEC 35, no. 2 (July 15, 2015): 312. http://dx.doi.org/10.35792/zot.35.2.2015.8569.
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THE EFFECT OF DRIED COW RUMEN FERMENTATION WITH TRICHODERMA VIRIDE ON METABOLIZABLE ENERGY VALUE OF BROILER. The purpose of this experiment was to determine the metabolizable energy of dried cow rumen. The experiment use dried cow rumen and dried cow rumen fermented Trichoderma viride during 9 days with 0,3% inoculum dose. The experiment use 18 six weeks old male broiler metabolizable energy parameter were analyzed with Wilcoxon test. The result of this experiment showed fermentation with Trichoderma viride could increase the metabolizable energy of dried cow rumen. Key word : Fermentation of dried cow rumen, broiler, metabolizable energy
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Nagadi, S., M. Herrero, and N. S. Jessop. "Effect of frequency of ovine ruminal sampling on microbial activity and substrate fermentation." Proceedings of the British Society of Animal Science 1999 (1999): 154. http://dx.doi.org/10.1017/s1752756200003094.
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Food eaten by a ruminant firstly undergoes microbial fermentation within the rumen. Nutritionally important characteristics of the food are the rate and extent of fermentation of its carbohydrate fraction, which can both be estimated using the in vitro gas production technique. The single greatest source of uncontrolled variation in any in vitro rumen fermentation system is the rumen fluid; curves produced from gas production data were influenced significantly by the variation in microbial activity between days (Menke and Steingass, 1988; Beuvink et al, 1992). A more reliable measure of rumen fluid activity is needed. The objective of this study was to determine whether the frequency of sampling of rumen fluid affected the microbial activity and subsequent fermentation.
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Walker, Charles E., James S. Drouillard, and Tiruvoor G. Nagaraja. "Optaflexx1 affects rumen fermentation." Kansas Agricultural Experiment Station Research Reports, no. 1 (January 1, 2007): 88–90. http://dx.doi.org/10.4148/2378-5977.1536.
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Castillo-González, AR, ME Burrola-Barraza, J. Domínguez-Viveros, and A. Chávez-Martínez. "Rumen microorganisms and fermentation." Archivos de medicina veterinaria 46, no. 3 (2014): 349–61. http://dx.doi.org/10.4067/s0301-732x2014000300003.
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Bagheri, M., G. R. Ghorbani, H. R. Rahmani, and M. Khorvash. "Effect of yeast and mannan-oligosaccharides on in vitro fermentation of different substrates." Proceedings of the British Society of Animal Science 2009 (April 2009): 91. http://dx.doi.org/10.1017/s1752756200029306.
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Results of yeast effects on in vitro rumen fermentation are inconsistent (Sullivan et al., 1999; Yang et al., 2004). In addition, there is no data on the effect of mannan-oligosacchrides (MOS) and their interaction with yeast on rumen fermentation. This trial was conducted to study the effects of yeast and MOS on rumen fermentation of different substrates.
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Wilk, Martyna, Ewa Pecka-Kiełb, Jerzy Pastuszak, Muhammad Umair Asghar, and Laura Mól. "Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production." Agriculture 12, no. 11 (November 18, 2022): 1943. http://dx.doi.org/10.3390/agriculture12111943.
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Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different types of copper supplement, their rumen decomposition and effect on in vitro ruminal fermentation as well as methanogenesis. Two different copper additives were used in the experiment: CS—copper sulfate (CuSO4 × 5 H2O)—and EC—encapsulated copper (tribasic copper chloride and copper sulfate enclosed within a polysaccharide polymer coating). A total mixed ration without copper additive was used as a control (C). In vitro rumen fermentation was conducted, and fermentation profile, gas production and methanogenesis were evaluated. After 24 h of fermentation, the amount of copper in the rumen fluid was significantly higher in the CS group. EC was protected against rumen degradation to a greater extent. The type of used copper supplement affects rumen fermentation. However, the effect on methanogenesis is ambiguous. CS supplement increases rumen gas production but does not affect methanogenesis. The obtained results suggest that the EC supplement may reduce the risk of low-fat milk and may improve the economic indicators of milk production. An in vivo experiment is necessary to compare the obtained in vitro results with animal productivity.
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Newbold, C. J., R. J. Wallace, and I. M. Nevison. "Influence of ionophores on in vitro fermentation by rumen fluid from sheep receiving yeast culture (Yeasacc; YC)." Proceedings of the British Society of Animal Production (1972) 1991 (March 1991): 78. http://dx.doi.org/10.1017/s0308229600020286.
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A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the rumen fermentation. These products have been shown to increase bacterial numbers within the rumen with an associated increase in the breakdown of fibre and supply of microbial protein (Williams and Newbold, 1990). YC has also been reported to increase the production of propionate in the rumen. Little appears to be known about the effect a combination YC and an ionophore would have on the rumen fermentation. This study describes the effects of the ionophores monensin and tetronasin on the fermentation of hay by rumen fluid from sheep fed a basal diet with or without YC.
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Candyrine, S. C. L., M. F. Jahromi, M. Ebrahimi, J. B. Liang, Y. M. Goh, and N. Abdullah. "In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids." Animal Production Science 57, no. 8 (2017): 1607. http://dx.doi.org/10.1071/an15684.
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An in vitro gas-production study was conducted to compare differences in rumen fermentation characteristics and the effect of supplementation of 4% linseed oil as a source of polyunsaturated fatty acids on the rumen fermentation profile in rumen fluid collected from goats and sheep. Rumen fluid for each species was obtained from two male goats of ~18 months old and two sheep of similar sex and age fed the similar diet containing 30% alfalfa hay and 70% concentrates. The substrate used for the fermentation was alfalfa hay and concentrate mixture (30:70) without (control) and with addition of linseed oil. The experiment was a two (inoculums) × two (oil levels) factorial experiment, with five replicates per treatment, and was repeated once. Rumen fermentation characteristics, including pH, fermentation kinetics, in vitro organic matter digestibility (IVOMD), volatile fatty acid (VFA) production and microbial population were examined. Results of the study showed that gas-production rate (c), IVOMD, VFA production and population of total bacteria and two cellulolytic bacteria (Ruminococus albus and Butyrivibrio fibrisolvens) from rumen fluid of goat were significantly (P &lt; 0.05) higher than those of samples from sheep. Irrespective of sources of inoculums, addition of oil did not affect fermentation capacity, IVOMD and total VFA production. The higher B. fibrisolvens population (associated with bio-hydrogenation) in rumen fluid of goat seems to suggest that polyunsaturated fatty acids are more prone to bio-hydrogention in the rumen of goat than in sheep. This assumption deserves further investigation.
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Li, Jinhui, Hui Yan, Jiaxin Chen, Chunhui Duan, Yunxia Guo, Yueqin Liu, Yingjie Zhang, and Shoukun Ji. "Correlation of Ruminal Fermentation Parameters and Rumen Bacterial Community by Comparing Those of the Goat, Sheep, and Cow In Vitro." Fermentation 8, no. 9 (August 28, 2022): 427. http://dx.doi.org/10.3390/fermentation8090427.
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In this study, we aimed to establish the correlation between ruminal fermentation parameters and the bacterial community by comparing those of the goat, sheep, and cow, thus illustrating the main bacteria causing the difference in rumen fermentation among goats, sheep, and cows and providing a new idea for improving the feed digestibility of ruminants. Rumen fluid from goats (Taihang White cashmere goat, n = 6), sheep (Hu sheep, n = 6), and cows (Holstein cow, n = 6) was collected using oral intubation and immediately brought back to the laboratory for a fermentation test with the same total mixed ration (TMR) feed in vitro. The rumen bacterial composition was measured by high-throughput sequencing of 16S rRNA genes in the MiSeq platform, the gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, and 48 h of fermentation, and the feed nutrient digestibility and the rumen fluid parameters were determined after 48 h of fermentation. The results showed that the 48 h GP of the sheep group was higher than that of the cow group (p < 0.05), and the theoretical maximum GP was higher than that of the goat and cow groups (p < 0.05). The organic matter digestibility (OMD), dry matter digestibility (DMD), crude protein digestibility (CPD), and gross energy digestibility (GED) of the sheep group were higher than those of the goat and cow groups (p < 0.05). The ammonia nitrogen (NH3-N), microbial protein (MCP), and total volatile fatty acids (TVFA) concentrations of the sheep group were higher than those of the other groups (p < 0.05), and the pH of the sheep group was lower than those of the other groups (p < 0.05). The 16S rRNA gene sequencing revealed that bacterial composition also differed in the rumens of the sheep, goat, and cow groups (ANOSIM, p < 0.05). We then used a random forest machine learning algorithm to establish models to predict the fermentation parameters by rumen bacterial composition, and the results showed that rumen bacterial composition could explain most of the ruminal fermentation parameter variation (66.56%, 56.13%, 65.75%, 80.85%, 61.30%, 4.59%, 1.41%, −3.13%, 34.76%, −25.62%, 2.73%, 60.74%, 76.23%, 47.48%, −13.2%, 80.16%, 4.15%, 69.03%, 32.29%, and 89.96% for 48 h GP, a (GP of quickly degraded part), b (GP of slowly degraded part), c (GP rate), a + b (theoretical maximum GP), DMD, OMD, GED, CPD, NDFD, ANDF, pH, NH3-N, MCP, acetic acid, propionic acid, butyric acid, valeric acid, TVFA, and A:P (acetic acid–propionic acid ratio), respectively). A correlation analysis revealed that Lactobacillus, Prevotellaceae_UCG-003, Selenomonas, Peptostreptococcus, and Olsenella significantly correlated with most in vitro fermentation parameters (p < 0.05). A comprehensive analysis showed that rumen fermentation parameters and bacterial composition differed in goats, sheep, and cows. The ruminal fermentation parameters of GP, a, b, c, a + b, pH, NH3-N, propionic acid, valeric acid, and A:P could be accurately predicted by rumen bacteria (explanation > 55% of variation), and the Lactobacillus, Prevotellaceae_UCG-003, Olsenella, Selenomonas, and Peptostreptococcus were the main bacteria that affected the in vitro fermentation parameters of goats, sheep, and cows.
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Wang, Liyan, Shoukun Ji, Hui Yan, Jinhui Li, Lishen Zhang, Dezhi Yan, Chunhui Duan, Yueqin Liu, and Yingjie Zhang. "Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH4 Emission and Skatole Content In Vitro." Fermentation 9, no. 5 (April 28, 2023): 428. http://dx.doi.org/10.3390/fermentation9050428.
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The purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH4) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula and immediately transferred to the laboratory for rumen fermentation in vitro. The experimental diet was supplemented with 0%, 0.2%, 0.8%, 1.2%, 1.8% and 2.4% FOS in the basal diet. Gas production (GP) and CH4 production were measured and recorded at 2, 4, 6, 8, 10, 12, 24, 36 and 48 h. After 48 h of fermentation, degradation rates of nutritional components, fermentation parameters and skatole content were determined. The results showed that the GP, the nutrient degradation rates and the fermentation parameters of rumen linearly increased with increasing doses of FOS supplementation (p < 0.05). There was a quadratic trend between FOS addition and CH4 production and skatole content in rumen fluid (p < 0.05). We also observed the CH4 production in the 1.2% FOS-treated group was significantly lower than the other FOS-treated groups. Skatole content of the 0.2%, 0.8% and 1.2% FOS-treated groups were significantly lower than the other FOS-treated groups (p < 0.05). Our findings indicated that the effect of FOS on rumen fermentation parameters, CH4 production and skatole production in vitro was dose-dependent. To improve the digestibility of nutrients and the fermentation parameters of rumen, a higher FOS dosage might be helpful. However, if CH4 and skatole production is a concern, a dose of FOS at 1.2% is recommended.
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Ungerfeld, Emilio M., M. Fernanda Aedo, Emilio D. Martínez, and Marcelo Saldivia. "Inhibiting Methanogenesis in Rumen Batch Cultures Did Not Increase the Recovery of Metabolic Hydrogen in Microbial Amino Acids." Microorganisms 7, no. 5 (April 27, 2019): 115. http://dx.doi.org/10.3390/microorganisms7050115.
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There is an interest in controlling rumen methanogenesis as an opportunity to both decrease the emissions of greenhouse gases and improve the energy efficiency of rumen fermentation. However, the effects of inhibiting rumen methanogenesis on fermentation are incompletely understood even in in vitro rumen cultures, as the recovery of metabolic hydrogen ([H]) in the main fermentation products consistently decreases with methanogenesis inhibition, evidencing the existence of unaccounted [H] sinks. We hypothesized that inhibiting methanogenesis in rumen batch cultures would redirect [H] towards microbial amino acids (AA) biosynthesis as an alternative [H] sink to methane (CH4). The objective of this experiment was to evaluate the effects of eight inhibitors of methanogenesis on digestion, fermentation and the production of microbial biomass and AA in rumen batch cultures growing on cellulose. Changes in the microbial community composition were also studied using denaturing gradient gel electrophoresis (DGGE). Inhibiting methanogenesis did not cause consistent changes in fermentation or the profile of AA, although the effects caused by the different inhibitors generally associated with the changes in the microbial community that they induced. Under the conditions of this experiment, inhibiting methanogenesis did not increase the importance of microbial AA synthesis as a [H] sink.
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Dewi, Wuryani Kusuma, Limbang Kustiawan Nuswantara, and Surono Surono. "Digestibility of Coconut Coir Fiber Fermented by Buffalo Rumen Fluid Microbes in Vitro." Jurnal Agripet 25, no. 1 (April 30, 2025): 95–100. https://doi.org/10.17969/agripet.v25i1.30112.
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This study aimed to examine the digestibility of fiber, including crude fiber, neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility of coconut husk fermented using selected buffalo rumen microbes with varying percentages of inoculum and fermentation periods. The study utilized a completely randomized design (CRD) with a factorial pattern (3×3) and 3 replications. The first factor was the percentage of inoculum (0%, 2.5%, and 5% mL of inoculum per gram of dry matter of coconut husk), and the second factor was the fermentation period (0, 7, and 14 days). The collected data were analyzed using analysis of variance (ANOVA) and, where applicable, Duncan’s multiple range test. The results indicated that there was no significant interaction (P0.05) between the different percentages of buffalo rumen fluid as inoculum and the varying fermentation periods on crude fiber and ADF digestibility of coconut husk. However, the percentage of buffalo rumen fluid as inoculum had a significant effect (P0.05) on crude fiber and ADF digestibility, and the fermentation period also significantly influenced (P0.05) ADF digestibility. An interaction between the percentage of buffalo rumen fluid and fermentation period had a significant effect (P0.05) on NDF digestibility. The findings concluded that the percentage of buffalo rumen fluid as inoculum and the fermentation period did not increase crude fiber and ADF digestibility; however, the interaction between 2.5% buffalo rumen fluid inoculum and a 14-day fermentation period resulted in improved NDF digestibility of coconut husk.
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YANG, H. J., H. ZHUANG, X. K. MENG, D. F. ZHANG, and B. H. CAO. "Effect of melamine onin vitrorumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures." Journal of Agricultural Science 152, no. 4 (October 15, 2013): 686–96. http://dx.doi.org/10.1017/s0021859613000725.
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SUMMARYThe effects of melamine on gas production (GP) kinetics, methane (CH4) production and fermentation of diets differing in forage content (low-forage (LF) diet: 200 g/kg and high-forage (HF) diet: 800 g/kg) by rumen micro-organismsin vitrowere studied using batch cultures. Rumen contents were collected from three Simmental×Luxi crossbred beef cattle. Melamine was added to the incubation bottles to achieve final concentration of 0 (control), 2, 6, 18, 54, 162 and 484 mg/kg of each diet. Cumulative GP was continuously measured in an automated gas recording instrument during 72 h of incubation, while fermentation gas end-products were collected to determine molar proportions of carbon dioxide (CO2), CH4and hydrogen gas (H2) in manually operated batch cultures. Differences in GP kinetics and fermentation gases were observed in response to the nature of the diets incubated. Although melamine addition did not affect GP kinetics and fermentation gas pattern compared to the control, the increase of melamine addition stimulated the yield of CH4by decreasing CO2, especially during the fermentation of the HF diet. The concentrations of ammonia nitrogen (N), amino acid N and microbial N in culture fluids were greater in the fermentation of LF- than HF diets, and these concentrations were increased by the increase of melamine addition after 72-h fermentation. The concentrations of total volatile fatty acids (VFA) were greater in HF than LF diets. The addition of melamine decreased total VFA concentrations and this response was greater in HF than LF diet fermentations. Melamine addition did not affect molar proportions of acetate, butyrate, propionate and valerate compared with the control; however, branched-chain VFA production, which was lower in the HF than the LF diet, was increased by the melamine addition, especially in the HF diet fermentation. The ratio of non-glucogenic to glucogenic acids was lower in the HF than the LF diet, but it was not affected by melamine addition. In brief, the greater reduction in the rate and extent of rumen fermentation found for the HF diet in comparison with the LF diet suggested that rumen fermentation rate and extentin vitrodepended mainly on the nature of the incubated substrate, and that they could be further inhibited by the increase of melamine addition.
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Hesni, V., A. Taghizadeh, H. Paya, H. Janmohamadi, G. A. Moghadam, and N. Pirani. "Effect of monensin and lasalocid on rumen fermentation in sheep." Proceedings of the British Society of Animal Science 2007 (April 2007): 221. http://dx.doi.org/10.1017/s1752756200021244.
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Ionophores, consisting of monensin and lasalocid were reported to improve rumen fermentation and metabolism of ruminants when delivered in their respective proper dosage (Mass et al., 2001). However, the efficacy of ionophores in the manipulation of rumen fermentation has been shown to vary with diet. Ionophores may also inhibit ruminal amino acid deamination (Yang et al., 2003). The objective of this study was to determine of the effect of monensin and lasalocid on rumen fermentation characteristics.
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Wang, Xinjie, Jianzhao Zhou, Runjie Jiang, Yuxuan Wang, Yonggen Zhang, Renbiao Wu, Xiaohui A, et al. "Development of an Alternative In Vitro Rumen Fermentation Prediction Model." Animals 14, no. 2 (January 17, 2024): 289. http://dx.doi.org/10.3390/ani14020289.
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The aim of this study is to identify an alternative approach for simulating the in vitro fermentation and quantifying the production of rumen methane and rumen acetic acid during the rumen fermentation process with different total mixed rations. In this experiment, dietary nutrient compositions (neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and dry matter (DM)) were selected as input parameters to establish three prediction models for rumen fermentation parameters (methane and acetic acid): an artificial neural network model, a genetic algorithm-bp model, and a support vector machine model. The research findings show that the three models had similar simulation results that aligned with the measured data trends (R2 ≥ 0.83). Additionally, the root mean square errors (RMSEs) were ≤1.85 mL/g in the rumen methane model and ≤2.248 mmol/L in the rumen acetic acid model. Finally, this study also demonstrates the models’ capacity for generalization through an independent verification experiment, as they effectively predicted outcomes even when significant trial factors were manipulated. These results suggest that machine learning-based in vitro rumen models can serve as a valuable tool for quantifying rumen fermentation parameters, guiding the optimization of dietary structures for dairy cows, rapidly screening methane-reducing feed options, and enhancing feeding efficiency.
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Yu, Jiangkun, Liyuan Cai, Jiacai Zhang, Ao Yang, Yanan Wang, Lei Zhang, Le Luo Guan, and Desheng Qi. "Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro." Microorganisms 8, no. 8 (July 30, 2020): 1160. http://dx.doi.org/10.3390/microorganisms8081160.
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This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.
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Vigh, Antal, Adriana Criste, Kévin Gragnic, Léa Moquet, and Christine Gerard. "Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro." Agriculture 13, no. 4 (April 16, 2023): 879. http://dx.doi.org/10.3390/agriculture13040879.
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The aim of this study was to assess the effects of supplementation with inorganic sources of manganese (MnO, MnSO4), zinc (ZnO, ZnSO4) and copper (CuSO4) at different levels (0.06%DM for Mn, 0.05%DM for Zn; 0.01 and 0.05%DM for Cu) on in vitro rumen fermentation, solubility and bioavailability. Fermentation activity was measured by total gas production (TGP) and dry matter degradability after 70 h of fermentation (dDM%). Trace mineral (TM) solubility was estimated via the TM concentration in the supernatant of the final fermentation medium (SOL) and TM bioavailability from the TM concentration in a bacterial-enriched fraction (BACT). Mn (regardless of source) and ZnO tended (p < 0.10) to decrease, while Cu showed no significant effect on TGP. The addition of inorganic Mn and of ZnO tended (p < 0.10) to decrease, ZnSO4 tended to increase (p < 0.10), whilst Cu showed no effect on dDM%. Concerning solubility, Mn (MnO and MnSO4), ZnSO4 and CuSO4 significantly (p < 0.05, p < 0.001 and p < 0.01) increased, while ZnO did not affect TM content in the SOL. These results indicate that MnSO4, ZnSO4 and CuSO4 are highly soluble, MnO is quite soluble, while ZnO has a low solubility in the rumen. Based on the TM content in BACT, MnO, MnS4 and CuSO4 have high bioavailability, while ZnO is poorly assimilated by rumen bacteria. However, the lack of clear inhibition or improvement in fermentations suggests that the rumen microbiota have a low requirement for TM supplementation.
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Qiu, Xinjun, Xiaoli Qin, Liming Chen, Zhiming Chen, Rikang Hao, Siyu Zhang, Shunran Yang, et al. "Serum Biochemical Parameters, Rumen Fermentation, and Rumen Bacterial Communities Are Partly Driven by the Breed and Sex of Cattle When Fed High-Grain Diet." Microorganisms 10, no. 2 (January 30, 2022): 323. http://dx.doi.org/10.3390/microorganisms10020323.
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Hybridization in bovines is practiced with the main aim of improving production performance, which may imply the microbial variations in the rumen from the parental breed cross to their progeny. Besides, the interactions of offspring breed with sex in terms of rumen bacteria are not clear. This study aims to evaluate the variations in rumen bacterial communities in different breeds and sexes, and the correlations among fattening performance, serum biochemical parameters, and rumen fermentation. Forty-two 19.2 ± 0.67-month-old beef cattle (390 ± 95 kg of initial body weight) comprising two genetic lines (Yiling and Angus × Yiling) and two sexes (heifers and steers) were raised under the same high-grain diet for 120 d. On the last two days, blood samples were collected from each animal via the jugular vein before morning feeding for analyzing serum biochemical parameters; rumen fluid samples were obtained via esophageal intubation 2 h after morning feeding for analyzing rumen fermentation parameters and bacterial communities. The results show that both breed and sex had a certain impact on fattening performance, serum biochemical parameters, and rumen fermentation. No differences in the diversity and structure of rumen bacterial communities were observed. Significant interactions (p < 0.05) of breed and sex were observed for Succinivibrionaceae UCG-002 and Prevotellaceae UCG-001. The relative abundances of the Rikenellaceae RC9 gut group, Prevotellaceae UCG-003, and Succinivibrio were different (p < 0.05) between breeds. Heifers had a higher (p = 0.008) relative abundance of the Rikenellaceae RC9 gut group than steers. Correlation analysis showed a significant relationship (p < 0.05) of rumen bacteria with serum biochemical parameters, rumen pH, and rumen fermentation patterns. Additionally, only two genera, Prevotellaceae UCG-003 and Prevotellaceae UCG-001, had positive correlations with feed efficiency. In conclusion, serum biochemical parameters, rumen fermentation, and rumen bacterial communities are partly driven by the breed and sex of cattle fed a high-grain diet.
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Huang, Jianzhi, Yueyun Sheng, Pengfei Xue, Donghui Yu, Peng Guan, Jiangang Ren, and Wenxi Qian. "Patterns of Spatial Variation in Rumen Microbiology, Histomorphology, and Fermentation Parameters in Tarim wapiti (Cervus elaphus yarkandensis)." Microorganisms 12, no. 1 (January 20, 2024): 216. http://dx.doi.org/10.3390/microorganisms12010216.
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The rumen is divided into multiple rumen sacs based on anatomical structure, and each has its unique physiological environment. Tarim wapiti preserved roughage tolerance after domestication, and adaptation to the desertified environment led to the development of a unique rumen shape and intraruminal environment. In this work, six Tarim wapiti were chosen and tested for fermentation parameters, microbes, and histomorphology in four rumen areas (Dorsal sac, DS; Ventral sac, VS; Caudodorsal blind sac, CDBS; Caudoventral blind sac, CVBS). Tarim wapiti’s rumen blind sac had better developed rumen histomorphology, the ventral sac was richer in VFAs, and the dominant bacteria varied most notably in the phylum Firmicutes, which was enriched in the caudoventral blind sac. The ventral sac biomarkers focused on carbohydrate fermentation-associated bacteria, the dorsal sac focused on N recycling, and the caudoventral blind sac identified the only phylum-level bacterium, Firmicutes; we were surprised to find a probiotic bacterium, Bacillus clausii, identified as a biomarker in the ventral sac. This research provides a better understanding of rumen fermentation parameters, microorganisms, and histomorphology in the Tarim wapiti rumen within a unique ecological habitat, laying the groundwork for future regulation targeting the rumen microbiota and subsequent animal production improvement.
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Cone, J. W., and M. A. M. Rodrigues. "Protein fermentation characteristics in rumen fluid determined with the gas production technique." Proceedings of the British Society of Animal Science 2009 (April 2009): 192. http://dx.doi.org/10.1017/s1752756200030313.
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The gas production technique was developed to determine the fermentation kinetics of organic matter in rumen fluid. However, the gas production technique can be adapted for the determination of protein fermentation characteristics. To do that the buffer must be N-free. All the N coming with the rumen fluid must be incorporated into microbial mass. This can be done by supplying the buffered rumen fluid with an excess of fast fermentable carbohydrates. To prevent a too high input of N from the rumen fluid the rumen fluid can be diluted further compared to the standard 3 times (Cone et al., 1996). This makes N the limiting factor for fermentation and the obtained gas production profiles reflect the availability of N from the feed samples. The aim of the present study was to investigate if the adapted gas production technique is suitable to determine differences in protein availability in rumen fluid. The fermentation characteristics of N of 19 feed samples were determined using the adapted gas production technique. The amount of sample incubated, was that sufficient to provide 15 mg N. The results were compared with data of N degradation obtained with the nylon bag technique.
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Rinttilä, Teemu, Colm A. Moran, and Juha Apajalahti. "DHA-Rich Aurantiochytrium Biomass, a Novel Dietary Supplement, Resists Degradation by Rumen Microbiota without Disrupting Microbial Activity." Applied Microbiology 2, no. 1 (January 7, 2022): 53–72. http://dx.doi.org/10.3390/applmicrobiol2010004.
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We first sought to evaluate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA), on rumen fermentation and the resistance of DHA to degradation and biohydrogenation by rumen microbes through ex vivo fermentation experiments. Subsequently, we sought to quantify the diet-derived DHA content of milk and the impact of AURA on microbial composition and metabolism in a pilot feeding trial with rumen-cannulated dairy cows. To achieve our aims, rumen fluid from cannulated cows was used as inoculum, and the effect of AURA inclusion on fermentation ex vivo was examined. At doses corresponding to the amount of AURA recommended for commercial production animals, only ~10% of DHA was degraded or biohydrogenated by rumen microorganisms. The results show that feeding with AURA had no effect on either total bacterial density or short-chain fatty acid production. Real-time quantitative PCR analysis of the rumen fluid samples collected during a seven-week in vivo trial revealed that microbes related to lactic acid metabolism and methanogenesis were significantly suppressed by the AURA-supplemented diet. The DHA concentration in milk increased over 25-fold with the AURA-supplemented diet and dropped by 30–40% within one week of washout. The addition of A. limacinum biomass to dairy cow diets resulted in positive effects on rumen microbial composition with no adverse effect on fermentation activity. AURA-derived DHA was stable, with only modest degradation in the rumen, and was successfully deposited in milk. This is the first study to investigate the effect of supplementing the diet of dairy cows with a protist-based biomass, namely, on important rumen fermentation parameters and on DHA deposition in milk, using a combination of ex vivo and in vivo approaches.
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Munyiva, Brenda, and Wahu Oyaya. "Effect of Rumen Fluid Dosage and Fermentation Time on Dissolved Protein Levels of Vegetable Waste Silage for Vannamei Shrimp Feed." International Journal Papier Advance and Scientific Review 2, no. 2 (October 7, 2021): 20–24. http://dx.doi.org/10.47667/ijpasr.v2i2.110.
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Specifically, the goal of this research was to evaluate the dissolved protein content of vegetable waste generated during the incubation of rumen fluid for use in (Whiteleg) vannamei shrimp diet. The results of the analysis of the degree of protein hydrolysis of vegetable waste treated with the addition of rumen fluid enzymes and different fermentation times of rumen fluid revealed that the addition of rumen fluid enzymes and different fermentation times of rumen fluid had a statistically significant effect (p0.05) on the degree of protein hydrolysis of vegetable waste. But there was no significant difference in the length of fermentation time or the interaction between the dosage of rumen fluid and the length of time (p>0.05) between the two groups. Duncan's test of rumen fluid dosage revealed that the degree of hydrolysis at a 1 percent dose was considerably greater (p0.05) than at 2 percent and 3 percent doses, and that the degree of hydrolysis at a 3 percent dose was significantly lower than at 2 percent
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Guo, Wei, Jolet K. van Niekerk, Mi Zhou, and Michael A. Steele. "PSIX-32 Assessment of Mucosa-associated Microbiota in the Colon and Rumen of Dairy Calves Fed High Plane of Milk and during Weaning Transition." Journal of Animal Science 98, Supplement_4 (November 3, 2020): 311. http://dx.doi.org/10.1093/jas/skaa278.554.
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Abstract The nature of weaning is considered as the most stressful period experienced by dairy calves and has been known to negatively affect calf growth and intake. The objectives of this study were to investigate the shift of rumen and colon mucosa-associated microbiota in dairy calves fed with a high plane of milk pre- and post-weaning, and whether such shifts are associated with rumen fermentation and growth performance. Six Holstein dairy bull calves were ruminally cannulated at the second week of life followed by weaning at the end of week 6. Ruminal and colon tissue samples were biopsied at the end of week 5, 7 and 12 to determine the bacterial community changes, and rumen content was collected at the same time to measure rumen fermentation parameters. QIIME2 was used to analyze microbial profiles and R studio software was used to determine the microbial changes and its relationship with phenotypic measures. Age-dependent profiles were observed for the colon mucosa associated microbiota but not for rumen mucosa associated microbiota, indicating that varied responses to weaning transition exists between colon and rumen mucosa associated microbiota. As calves aged, higher abundance of taxa such as Delftia (associated with hexadecanoic acid production) and lower abundance of Veillonella (associated with short-chain organic acids utilization) were detected in the colon mucosa. The rumen also displayed taxonomic changes with decreases in Pseudobutyrivibrio in older calves. In addition, we identified significantly positive correlations between Prevotella associated with rumen mucosa and average daily gain, Isovalerate and Isobutyrate, suggesting that this genus may promote rumen fermentation during weaning transition. Our findings suggest that weaning transition affects rumen and colon mucosa-associated bacteria, and some of the rumen mucosa-associated microbiota promote rumen fermentation during this period, which may have long-term effects on animal health and productivity.
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Calsamiglia, S., P. Cardozo, A. Ferret, and A. Bach. "Changes in rumen microbial fermentation during acidosis are due to a combined effects of fermentation substrate and pH." Proceedings of the British Society of Animal Science 2007 (April 2007): 21. http://dx.doi.org/10.1017/s1752756200019244.
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The supply of high-concentrate diets results in the reduction in ruminal pH and the development of acidosis. Because the reduction of pH occurs at the same time as the diet is changed, the effects are confounded. For example, high-concentrate diets ferment towards propionate, and low rumen pH also results in greater propionate production. Which factor is responsible for the increase in rumen propionate? The objective of this study was to determine the effects of rumen pH and the type of diet (D) on rumen microbial fermentation, with the aim of developing simple mathematical model to describe these effects.
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Guo, Yanxia, Faiz-ul Hassan, Mengwei Li, Huade Xie, Lijuan Peng, Zhenhua Tang, and Chengjian Yang. "Effect of Sodium Nitrate and Cysteamine on In Vitro Ruminal Fermentation, Amino Acid Metabolism and Microbiota in Buffalo." Microorganisms 10, no. 10 (October 14, 2022): 2038. http://dx.doi.org/10.3390/microorganisms10102038.
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Nitrate is used as a methane inhibitor while cysteamine is considered as a growth promoter in ruminants. The present study evaluated the effect of sodium nitrate and cysteamine on methane (CH4) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. Four treatments containing a 0.5 g of substrate were supplemented with 1 mg/mL sodium nitrate (SN), 100 ppm cysteamine hydrochloride (CS), and a combination of SN 1 mg/mL and CS 100 ppm (CS+SN), and a control (no additive) were applied in a completely randomized design. Each treatment group had five replicates. Two experimental runs using in vitro batch culture technique were performed for two consecutive weeks. Total gas and CH4 production were measured in each fermentation bottle at 3, 6, 9, 12, 24, 48, and 72 h of incubation. The results showed that SN and CS+SN reduced the production of total gas and CH4, increased the rumen pH, acetate, acetate to propionate ratio (A/P), and microbial protein (MCP) contents (p < 0.05), but decreased other volatile fatty acids (VFA) and total VFA (p = 0.001). The CS had no effect on CH4 production and rumen fermentation parameters except for increasing A/P. The CSN increased the populations of total bacteria, fungi, and methanogens but decreased the diversity and richness of rumen microorganisms. In conclusion, CS+SN exhibited a positive effect on rumen fermentation by increasing the number of fiber degrading and hydrogen-utilizing bacteria, with a desirable impact on rumen fermentation while reducing total gas and CH4 production.
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Qian, Wenxi, ZhiPeng Li, Weiping Ao, Guangyong Zhao, Guangyu Li, and JianPing Wu. "Bacterial community composition and fermentation in the rumen of Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries)." Canadian Journal of Microbiology 63, no. 5 (May 2017): 375–83. http://dx.doi.org/10.1139/cjm-2016-0596.
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The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rumen fermentation of these animals are poorly understood. Here, we apply high-throughput sequencing to examine the bacterial community in the rumen of cattle, red deer, and sheep and measured rumen fermentation products. Overall, 548 218 high-quality sequences were obtained and then classified into 6034 operational taxonomic units. Prevotella spp., Succiniclasticum spp., and unclassified bacteria within the families Succinivibrionaceae, Lachnospiraceae, and Veillonellaceae were the dominant bacteria in the rumen across the 3 hosts. Principal coordinate analysis identified significant differences in the bacterial communities across the 3 hosts. Pseudobutyrivibrio spp., Oscillospira spp., and Prevotella spp. were more prevalent in the rumen of the cattle, red deer, and sheep, respectively. Among the 3 hosts, the red deer rumen had the greatest amounts of acetate and butyrate and the lowest pH value. These results showed that Prevotella spp. are the dominant bacteria in the rumen of the cattle, red deer, and sheep, providing new insight into the rumen fermentation of ruminants distributed in the Tarim Basin.
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Durix, Andrée, C. Jean-Blain, H. P. Sallmann, and J. P. Jouany. "Use of a semicontinuous culture system (RUSITEC) to study the metabolism of ethanol in the rumen and its effects on ruminal digestion." Canadian Journal of Animal Science 71, no. 1 (March 1, 1991): 115–23. http://dx.doi.org/10.4141/cjas91-013.
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The metabolism of ethanol by rumen micro-organisms and its effects on rumen fermentations have been studied in vitro in a semicontinuous fermentor (RUSITEC). Ethanol introduced in the fermentor at 1, 4 or 8 g d−1 L−1 of rumen juice induced important qualitative and quantitative modifications in the fermentation outputs. Total VFA production was increased from 5 to 40% according to the diet and ethanol concentration. Relative proportions of VFA were modified: caproate concentration increased three times, propionate and isovalerate concentrations decreased significantly in most cases. Methane production was increased. Whatever the ethanol concentration and the type of diet, in vitro digestibility parameters and end-products from solid feedstuffs stayed practically unchanged by ethanol addition. Except with the lowest supply (1 g d−1 L−1), ethanol induced an uncoupling effect on the metabolism of rumen bacteria and decreased biomass production. Radioactivity of 2-14C ethanol was recovered mainly in acetate (77–80%). Microbial transformation of ethanol in RUSITEC was limited regardless of ethanol concentration. Ethanol had a negligible effect on the digestibility of solid feedstuffs but induced consistent qualitative changes in rumen fermentations. Key word: Ethanol, metabolism, rumen, semicontinuous culture
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Ismartoyo, Ismartoyo, Rohmiyatul Islamiyati, and Muhammad Rusdy. "Rumen Fermentation of Local Grasses Feed to Native Goat." Hasanuddin Journal of Animal Science (HAJAS) 5, no. 1 (February 7, 2024): 28–35. http://dx.doi.org/10.20956/hajas.v5i1.24777.
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The aim of this research was to examine the feed rumen fermentation in the rumen of goat fed 4 different diets. This study was designed based on the Latin Square Design which consists of 4 diet treatments with 4 replications in each diet treatment. A total of 4 male goats, with relatively the same weight and age, were randomly assigned to an individual metabolic cage fed with 4 diets. The four diets studied were R1: Elephant grass, R2: Mini elephant grass, R3: Panicum maximum grass and R4: Brachiaria decumbens grass. Each diet was added with 20% of rice bran. The result of this experiment indicated that there was no any significant effect of different diets on the pH values, concentrations of VFA (Volatile fatty acids), and Ammonia (NH3) in the rumen of goat. The pH values in the rumen of goat treated with R1, R2, R3, and R4 were 7.17, 7.12, 7.07, and 7.0, respectively. The concentration of ammonia (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 4.6, 3.3. 4.5, and 4.1, respectively. Whereas the level of VFA (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 40.4, 43.6, 48.7, and 49.9, respectively. There was no any significantly difference (p>0.05) in the characteristic of rumen fermentation between the diet treatment. This might be due to the similarity in the nutrient content of those grasses studied. The characteristic of rumen fermentation was in the range of optimal growth of rumen microbes. It is concluded that those of four grasses studied edible, acceptable and contain a good nutrient as a feed resource for goat. The nutrient content of the grasses studied creates a good rumen environment for optimum feed fermentation in the rumen of goat.
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Dhakal, Rajan, André Luis Alves Neves, Rumakanta Sapkota, Prabhat Khanal, and Hanne Helene Hansen. "Prokaryote Composition and Structure of Rumen Fluid before and after In Vitro Rumen Fermentation." Fermentation 10, no. 2 (February 14, 2024): 108. http://dx.doi.org/10.3390/fermentation10020108.
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Background: This study aimed to investigate the impact of in vitro rumen fermentation (IVRF) on the microbiome structure and composition of rumen fluid before and after fermentation assays. Methods and Results: Six separate fermentation batches were run for 48 h using maize silage as the basal feed. Rumen fluid samples were analyzed before (RF; only rumen fluid inoculant) and after 48 h fermentation assay (MS; maize silage as the substrate) and further processed for microbiome analysis using amplicon sequencing targeting the V4 region of the bacterial 16S rRNA gene. Bacterial alpha diversity revealed that the Shannon index and observed index were similar between MS and RF fluid. The core microbiome was detected in 88.6% of the amplicon sequence variants in MS and RF. Taxonomic analysis at the phylum level showed similar abundances of Bacteroidetes, Proteobacteria, Firmicutes, Verrucomicrobiota, Spirochaetota, Patescibacteria, and Campilobacterota in MS and RF. The Bray–Curtis distance matrix showed similar bacterial community structure among MS and RF samples. Conclusion: Our results indicated that the in vitro procedure did not affect the bacterial community structure compared to the original rumen fluid inoculum. It should be noted that assessing the microbiome at a single endpoint (i.e., 48 h) may not provide a comprehensive understanding of the microbiome profile dynamics. However, the findings of this study provide a basis for future microbiome-based in vitro fermentation tests and confirm that the technique allows a high degree of species diversity that approximates the rumen function in vivo.
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Ouda, J. O., C. J. Newbold, S. Lopez, N. Nelson, A. R. Moss, R. J. Wallace, and H. Omed. "The effect of acrylate and fumarate on fermentation and methane production in the rumen simulating fermentor (Rusitec)." Proceedings of the British Society of Animal Science 1999 (1999): 37. http://dx.doi.org/10.1017/s1752756200001927.
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Methane formation in the rumen represents a substantial loss of energy to the animal and is also a major source of greenhouse gas emissions from agriculture. Methanogenesis is the main means of disposal of hydrogen during rumen fermentation. The formation of propionate represents an alternative route of hydrogen disposal, providing sufficient propionate precursors are available. Theoretically, adding propionate precursors should stimulate propionate production and decrease methane production. In the present experiment, the effects of two potential precursors of propionate, fumarate and acrylate, on rumen fermentation and methane production were investigated in a rumen simulating fermentor.
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TKALCIC, SUZANA, CATHY A. BROWN, BARRY G. HARMON, ANANT V. JAIN, ERIC P. O. MUELLER, ANDREW PARKS, KAREN L. JACOBSEN, SCOTT A. MARTIN, TONG ZHAO, and MICHAEL P. DOYLE. "Effects of Diet on Rumen Proliferation and Fecal Shedding of Escherichia coli O157:H7 in Calves." Journal of Food Protection 63, no. 12 (December 1, 2000): 1630–36. http://dx.doi.org/10.4315/0362-028x-63.12.1630.
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Calves inoculated with Escherichia coli O157:H7 and fed either a high-roughage or high-concentrate diet were evaluated for rumen proliferation and fecal shedding of E. coli O157:H7. Calves fed the high-roughage diet had lower mean rumen volatile fatty acid concentrations and higher rumen pH values than did calves fed the high-concentrate diet. Despite these differences in rumen conditions, the calves fed the high-roughage diet did not have greater rumen populations of E. coli O157: H7 and did not exhibit increased or longer fecal shedding compared with the calves fed the high-concentrate diet. Two calves shedding the highest mean concentrations of E. coli O157:H7 were both fed the high-concentrate diet. There was a significant (P &lt; 0.05) positive correlation between fecal shedding and rumen volatile fatty acid concentration in calves fed a high-concentrate diet. The effects of diet on E. coli O157:H7 proliferation and acid resistance were investigated using an in vitro rumen fermentation system. Rumen fluid collected from steers fed a high-roughage diet, but not from steers fed a high-concentrate diet, supported the proliferation of E. coli O157:H7. Rumen fluid from steers fed a high-concentrate diet rapidly induced acid resistance in E. coli O157:H7. The impact of diet on fecal shedding of E. coli O157:H7 is still unclear and may depend on dietary effects on fermentation in the colon and on diet-induced changes in the resident microflora. However, rapid development of acid tolerance by E. coli O157:H7 in the rumens of calves fed high-concentrate diets, allowing larger populations to survive passage through the acidic abomasum to proliferate in the colon, may be one factor that influences fecal shedding in cattle on feed.
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Fakhri, S., A. R. Moss, D. I. Givens, and E. Owen. "Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds." Proceedings of the British Society of Animal Science 1997 (1997): 196. http://dx.doi.org/10.1017/s1752756200596379.
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Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.
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Hussain, A., and E. L. Miller. "Effect of supplementation of sucrose and lactose with sodium bicarbonate on rumen metabolism and microbial protein synthesis in sheep." Proceedings of the British Society of Animal Science 1999 (1999): 28. http://dx.doi.org/10.1017/s1752756200001836.
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Sucrose and lactose are used as energy supplements in ruminant diets. In our previous study (Hussain and Miller, 1998) lactose maintained a higher rumen pH, increased organic matter and neutral detergent fibre digestion in the rumen, reduced the number of rumen protozoa and increased microbial (bacterial) protein flow to the duodenum compared with sucrose. However, it was not clear whether the effect of lactose on pH or on reduction of protozoa was the main factor increasing rumen fermentation and microbial protein flow. The objective of the present study was to examine the effect of isoenergetic amounts of sucrose and lactose as supplements (equivalent to 50 g glucose) on rumen fermentation and metabolism in the presence of sodium bicarbonate to maintain rumen pH.
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Astuti, W. D., Y. Widyastuti, E. Wina, S. Suharti, R. Ridwan, and K. G. Wiryawan. "Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR." Journal of the Indonesian Tropical Animal Agriculture 43, no. 2 (May 24, 2018): 184. http://dx.doi.org/10.14710/jitaa.43.2.184-192.
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The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source.
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CIESLAK, A., P. ZMORA, A. STOCHMAL, L. PECIO, W. OLESZEK, E. PERS-KAMCZYC, J. SZCZECHOWIAK, A. NOWAK, and M. SZUMACHER-STRABEL. "Rumen antimethanogenic effect ofSaponaria officinalisL. phytochemicalsin vitro." Journal of Agricultural Science 152, no. 6 (April 8, 2014): 981–93. http://dx.doi.org/10.1017/s0021859614000239.
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SUMMARYAlthough the effect of saponins or saponin-containing plants on rumen microorganisms and rumen fermentation has been intensively investigated, this issue still requires special attention. Many of the phenomena occurring in the rumen related to dietary saponin supplementation are still not fully understood.Saponaria officinalisis a triterpenoid saponin-containing plant; thus, the aim of the present study was to evaluate the effect ofS. officinalisL. powdered root, methanolic extract of theS. officinalisroot (SOR) and the effect of the separated fractions (polysaccharides, saponins and phenolics) ofS. officinalison rumen methanogenesis, microbial population and rumen fermentation characteristics in anin vitrobatch culture fermentation system. The powdered root (raw plant material) andS. officinalisextract (SOE) decreasedin vitromethane production and consequently reduced the microbial population in a dose-dependent manner. The inhibition of methanogenesis was accompanied by changes in the volatile fatty acids profile.In vitrodry matter digestibility was not affected by any of the secondary compounds applied. The highest applied doses of SOE caused a higher reduction in methanogenesis (33·5v. 14·4%) than the highest doses of powdered root form. Such results suggest that the basic components of the SOR could interact with phytochemicals or that the phytochemicals became physically less available for microbiota, resulting in a decreased antimethanogenic activity of the powdered rootv. the extract. Among all the fractions selected, the saponin fraction exerted the greatest impact on ruminal fermentation. In conclusion, saponins decreased methane production by 29% in comparison with the control. This decrease was related to the reduction in protozoa and methanogen counts. It is proposed thatS. officinalishas the potential to inhibit rumen methanogenesis without affecting rumen fermentation adversely.
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Wei, Xiao, Kehui Ouyang, Tanghui Long, Zuogui Liu, Yanjiao Li, and Qinghua Qiu. "Dynamic Variations in Rumen Fermentation Characteristics and Bacterial Community Composition during In Vitro Fermentation." Fermentation 8, no. 6 (June 14, 2022): 276. http://dx.doi.org/10.3390/fermentation8060276.
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This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), fermentation at 12 h (R12, n = 10), and fermentation at 24 h (R24, n = 10). Results showed that gas production, concentrations of microbial crude protein, ammonia nitrogen, and individual volatile fatty acids (VFA), as well as total VFA and branched-chain VFA concentrations, were higher in R24 when compared with R12 (p < 0.05). However, no significant differences were observed in acetate to propionate ratio and fermentation efficiency between R12 and R24 (p > 0.05). Bacterial diversity analysis found that Shannon index and Simpson index were higher in R24 (p < 0.05), and obvious clusters were observed in rumen bacterial community between R12 and R24. Taxonomic analysis at the phylum level showed that the abundances of Proteobacteria and Fibrobacteres were higher in R12 than that in R24, and inverse results were observed in Bacteroidetes, Firmicutes, Cyanobacteria, Verrucomicrobia, Lentisphaerae, and Synergistetes abundances. Taxonomic analysis at the genus level revealed that the abundances of Rikenellaceae RC9 gut group, Succiniclasticum, Prevotellaceae UCG-003, Christensenellaceae R-7 group, Ruminococcaceae UCG-002, Veillonellaceae UCG-001, and Ruminococcaceae NK4A214 group were higher in R24, whereas higher abundances of Succinivibrionaceae UCG-002, Ruminobacter, and Fibrobacter, were found in R12. Correlation analysis revealed the negative associations between gas production and abundances of Proteobacteria, Succinivibrionaceae UCG-002, and Ruminobacter. Moreover, the abundances of Firmicutes, Rikenellaceae RC9 gut group, Christensenellaceae R-7 group, and Ruminococcaceae UCG-002 positively correlated with VFA production. These results indicate that both rumen fermentation characteristics and bacterial community composition were dynamic during in vitro fermentation, whereas the fermentation pattern, efficiency, and bacterial richness remained similar. This study provide insight into the dynamics of rumen fermentation characteristics and bacterial composition during in vitro fermentation. This study may also provide a reference for decision-making for the sampling time point when conducting an in vitro fermentation for bacterial community investigation.
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Wu, Yinglian, Chong Jiao, Qiyu Diao, and Yan Tu. "Effect of Dietary and Age Changes on Ruminal Microbial Diversity in Holstein Calves." Microorganisms 12, no. 1 (December 20, 2023): 12. http://dx.doi.org/10.3390/microorganisms12010012.
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Ruminal microorganisms play a crucial role in the energy supply of ruminants and animal performance. We analyzed the variations in rumen bacteria and fungi at 45 d, 75 d, and 105 d by using 16SrRNA and ITS sequencing data and investigated their correlation with rumen fermentation. According to the results, rumen microflora tended to gradually mature with age, and bacterial and fungal establishment gradually stabilized. Upon comparing the three periods, the concentration of propionic acid increased significantly (p < 0.05) after weaning, and weaning accompanied by a transition in diet remarkably decreased (p < 0.05) rumen diversity in the short term and induced a corresponding change in the rumen microbiota composition. Bacteroidota, Actinobacteriota, and Firmicutes were the core bacterial phyla for all age periods. Ruminococcus, NK4A214_group, Sharpea, Rikenellaceae_RC9_gut_group, and norank_f__Butyricicoccaceae were the markedly abundant bacterial genera in pre-weaning. After weaning, the relative abundance of Erysipelotrichaceae_ UCG-002, Eubacterium_ruminantium_group, and Solobacterium significantly increased (p < 0.05). The relative abundance of Acetitomaculum increased with age with the greatest abundance noted at 105 d (37%). The dominant fungal phyla were Ascomycota and Basidiomycota, and Aspergillus and Xeromyces were the most abundant fungal genera after weaning. Trichomonascus, Phialosimplex, and Talaromyces were enriched at 105 d. However, the low abundance of Neocallimastigomycota was not detected throughout the study, which is worthy of further investigation. In addition, correlations were observed between age-related abundances of specific genera and microbiota functions and rumen fermentation-related parameters. This study revealed that rumen microbiota and rumen fermentation capacity are correlated, which contributed to a better understanding of the effects of age and diet on rumen microbiology and fermentation in calves.
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Li, Qin, Yan Tu, Tao Ma, Kai Cui, Jianxin Zhang, Qiyu Diao, and Yanliang Bi. "Effects of Two Feeding Patterns on Growth Performance, Rumen Fermentation Parameters, and Bacterial Community Composition in Yak Calves." Microorganisms 11, no. 3 (February 24, 2023): 576. http://dx.doi.org/10.3390/microorganisms11030576.
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The health of young ruminants is highly dependent on early rumen microbial colonization. In this study, the effects of milk replacer on growth performance, rumen fermentation, and the rumen microflora in yak calves were evaluated. Sixty yak calves (body weight = 22.5 ± 0.95 kg, age = 30 ± 1 d) were assigned to the CON group (breastfed) or TRT group (milk replacer fed) and evaluated over 120 d. At 120 d, ruminal fluid samples were collected from 14 calves and then conducted for rumen fermentation and microbiota analyses. There was no difference in growth performance; however, calf survival was higher in the TRT group than in the CON group. The concentration of total volatile fatty acids and the molar proportion of butyric acid and lactic acid were increased with milk replacer feed in the TRT group (p < 0.05), but iso-valeric acid concentration was highest in the CON group (p < 0.05). Firmicutes and Bacteroidetes were the most dominant phyla in the CON and TRT groups, respectively. In the TRT group, Bacteroidetes, Prevotellaceae, Bacteroidia, Bacteroidetes, and Prevotella_1 were the dominant flora in the rumen of calves. The relative abundances of various taxa were correlated with rumen fermentation parameters; the relative abundance of Quinella and iso-butyrate levels were positively correlated (r = 0.57). The relative abundances of the Christensenellaceae_R-7_group and A/P were positively correlated (r = 0.57). In summary, milk replacer is conducive to the development of the rumen microflora, the establishment of rumen fermentation function, and the implementation of early weaning in yaks.
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Newbold, C. J., and R. J. Wallace. "The effect of yeast and distillery by-products on the fermentation in the rumen simulation technique (rusitec)." Proceedings of the British Society of Animal Production (1972) 1992 (March 1992): 210. http://dx.doi.org/10.1017/s0308229600023199.
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The practice of adding low levels of non-commensal yeast and fungi to ruminant diets is increasingly gaining acceptance as a means of manipulating rumen fermentation to benefit production. Reported benefits include an increased degradability of forages in the rumen and an improved flow of microbial protein from the rumen (Williams and Newbold, 1990).Distillery by-products, such as pot ale syrup, are commonly included in ruminant diets as an energy source. However, pot ale syrup contains a substantial number of yeast cells. The aim of the present study was to establish if different yeasts and yeast-containing by-products had similar effects on rumen fermentation to those found with a commercial yeast culture feed additive.Two commercial Saccharomyces cerevisiae preparations (Alkosel, Alko Biotechnology, Finland and Yea-sacc, Alltech, UK), active dried baker's yeast (United Distillers, UK) and two pot ale syrups from the Inchgower and Dailuaine distilleries were compared for their effects on the fermentation in the rumen simulation technique (Rusitec).
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Rabee, Alaa Emara, Khalid Z. Kewan, Hassan M. El Shaer, Mebarek Lamara, and Ebrahim A. Sabra. "Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep." AIMS Microbiology 8, no. 1 (2022): 26–41. http://dx.doi.org/10.3934/microbiol.2022003.
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<abstract> <p>Rumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (<italic>Phoenix dactylifera</italic>) and olive (<italic>Olea europaea</italic>) trees have received attention to be used in animal feeding. This study evaluated the impact of non-traditional feed resources including olive cake (OC), discarded dates (DD), and date palm frond (DPF) in sheep diet on rumen fermentation, diversity and relative abundance of rumen methanogens. Nine adult rams were assigned to three equal groups and fed three diets: traditional concentrates mixture (S1); non-traditional concentrate mixture (S2) based on DD and OC; and (S3) composed of the same S2 concentrate supplemented with DPF as a roughage part. The results showed that rumen pH was higher with S3 diet than the other two diets. However, the S1 diet showed the highest values of total volatile fatty acids (TVFA) and rumen ammonia. In addition, the proportions of acetic and butyric acids were increased, whereas propionic acid declined in S2 and S3 compared to the S1 diet. Rumen methanogens were dominated by <italic>Methanobrevibacter</italic> that showed a numeric decline by including DD, OC, and DPF in the animal diets. Principal component analysis (PCA) based on rumen fermentation parameters and relative abundances of methanogens genera showed three distinct clusters. Also, positive and negative correlations were revealed between methanogens genera and rumen metabolites. This study expands the knowledge regarding the effect of agricultural byproducts on rumen fermentation and the methanogenic community.</p> </abstract>
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Kingston-Smith, Alison H., Joan E. Edwards, Sharon A. Huws, Eun J. Kim, and Michael Abberton. "Plant-based strategies towards minimising ‘livestock's long shadow’." Proceedings of the Nutrition Society 69, no. 4 (August 4, 2010): 613–20. http://dx.doi.org/10.1017/s0029665110001953.
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Ruminant farming is an important component of the human food chain. Ruminants can use offtake from land unsuitable for cereal crop cultivation via interaction with the diverse microbial population in their rumens. The rumen is a continuous flow fermenter for the digestion of ligno-cellulose, with microbial protein and fermentation end-products incorporated by the animal directly or during post-ruminal digestion. However, ruminal fermentation is inefficient in capturing the nutrient resource presented, resulting in environmental pollution and generation of greenhouse gases. Methane is generated as a consequence of ruminal fermentation and poor retention of ingested forage nitrogen causes nitrogenous pollution of water and land and contributes to the generation of nitrous oxide. One possible cause is the imbalanced provision of dietary substrates to the rumen micro-organisms. Deamination of amino acids by ammonia-producing bacteria liberates ammonia which can be assimilated by the rumen bacteria and used for microbial protein synthesis. However, when carbohydrate is limiting, microbial growth is slow, meaning low demand for ammonia for microbial protein synthesis and excretion of the excess. Protein utilisation can therefore be improved by increasing the availability of readily fermentable sugars in forage or by making protein unavailable for proteolysis through complexing with plant secondary products. Alternatively, realisation that grazing cattle ingest living cells has led to the discovery that plant cells undergo endogenous, stress-mediated protein degradation due to the exposure to rumen conditions. This presents the opportunity to decrease the environmental impact of livestock farming by using decreased proteolysis as a selection tool for the development of improved pasture grass varieties.
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Nueraihemaiti, Gulinizier, Xiangdong Huo, Huiying Zhang, Honglin Shi, Yan Gao, Jun Zeng, Qing Lin, and Kai Lou. "Effect of Diet Supplementation with Two Yeast Cultures on Rumen Fermentation Parameters and Microbiota of Fattening Sheep In Vitro." Microorganisms 13, no. 3 (February 28, 2025): 550. https://doi.org/10.3390/microorganisms13030550.
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Yeast culture can improve ruminant health and reduce economic losses in intensive farming, but as a non-standardized product in China, its quality and efficacy vary significantly. In this study, a self-developed yeast culture was compared with a commercially available product using in vitro rumen fermentation and amplicon-based high-throughput sequencing to evaluate its effects on rumen fermentation parameters, microbial diversities, and community compositions in Hu sheep. The aim was to validate the efficacy and mechanisms of the self-developed yeast culture, produced with simplified raw materials and processes, on rumen function. The experiment was divided into four groups. In each 60 mL fermentation solution, the following treatments were added: 0.00 g high-concentrate diet (CK1 group, blank control), 0.40 g high-concentrate diet (CK2 group, basal diet control), 0.40 g high-concentrate diet supplemented with 5% XP yeast culture (XP group), and 0.40 g high-concentrate diet supplemented with 5% YC yeast culture (YC group). Gas production was measured every 4 h during fermentation. At the end of fermentation, pH, ammonia nitrogen, microbial protein, volatile fatty acids, and ruminal microbiota were determined. The results demonstrated the following. Compared to the CK2 group, both the XP and YC groups exhibited a significant increase (p < 0.05) in cumulative gas production and microbial protein content, while a significant decrease (p < 0.05) was observed in acetic acid content and the acetate-to-propionate ratio. The microbial protein content in the YC group was significantly higher (p < 0.05) than that in the XP group. Additionally, the content of valeric acid and isobutyric acid in the XP group was significantly higher (p < 0.05) compared to the other groups. The microbial community sequencing results revealed that the addition of yeast culture did not affect the alpha diversity index of rumen bacteria (p > 0.05); however, the addition of XP significantly reduced (p < 0.05) the richness of rumen fungal communities. At the phylum and genus levels, the relative abundance of multiple functional bacteria improved after adding YC. In summary, under the conditions of in vitro rumen fermentation with high-concentrate diets, adding 5% XP and YC yeast cultures both promoted rumen fermentation. The rumen fermentation type changed from the acetic acid type to the propionic acid type, which regulated rumen microbial composition and thereby improved dietary digestion efficiency. Notably, YC significantly increased the relative abundance of functional microbial communities compared to XP. These findings provide a theoretical and practical foundation for optimizing the large-scale breeding of Hu sheep.
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Xue, Ligang, Shuyi Zhou, Dan Wang, Fangyu Zhang, Junfeng Li, and Liyuan Cai. "The Low Dose of Saccharomyces cerevisiae Is Beneficial for Rumen Fermentation (Both In Vivo and In Vitro) and the Growth Performance of Heat-Stressed Goats." Microorganisms 10, no. 10 (September 20, 2022): 1877. http://dx.doi.org/10.3390/microorganisms10101877.
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This study aimed to investigate the effects of Saccharomyces cerevisiae on rumen fermentation and the growth performance of heat-stressed goats. The fermentation experiment was conducted using Saccharomyces cerevisiae added at 0‰ (HS1), 0.30‰ (SC1), 0.60‰ (SC2), and 1.20‰ (SC3) of the dry matter (DM) weight of the basal diet. The results showed that supplementing with 0.60‰ (SC2) could increase the pH, acetic acid to propionic acid ratio, the concentrations of ammonia nitrogen, total volatile fatty acids, acetic acid, propionic acid, butyric acid, and the degradability of DM, neutral detergent fiber, and acid detergent fiber in rumen fluids of heat-stressed goats. In the feeding experiment, twelve heat-stressed goats were assigned to a 4 × 4 Latin square experimental design, and the Saccharomyces cerevisiae supplement levels are similar to the fermentation experiment above. Similar effects on rumen fermentation and digestibility parameters were obtained with a supplement with 0.60‰ of Saccharomyces cerevisiae (SC2A) compared to the fermentation trial. Moreover, in the SC2A group, the DM intake and average daily gain also increased significantly compared with other groups. These results suggested that a low dose of Saccharomyces cerevisiae can still effectively improve the rumen fermentation and growth performance of heat-stressed goats.
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Mantovani, Hilario. "90 Microbiome-Derived Bioactive Molecules to Reduce Enteric Methane Emissions." Journal of Animal Science 101, Supplement_2 (October 28, 2023): 234–35. http://dx.doi.org/10.1093/jas/skad341.264.
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Abstract Microorganisms are pivotal for the development, health, and productivity of livestock. In adult ruminants, the microbiota colonizing the rumen is essential for efficient depolymerization of indigestible carbohydrates from plant biomass and their conversion into microbial protein and fermentation end-products that are used by the host for growth. In the last decades, next-generation sequencing and omics technologies provided unparalleled insights into the composition, structure, and function of the gastrointestinal (GI) tract microbiome of ruminants and helped improve our understanding of relationships between the rumen microbiota with cattle performance (efficiency) traits, as well as health and disease and greenhouse gas emissions. The ecological interactions between microbes within the rumen ecosystem are complex and involve, among others, cross-feeding, predation, parasitism, antagonism, and competition for novel (empty) niches and available resources. These diverse associations of ecological traits across distinct microbial populations that coexist in the same ecosystem represent a goldmine for the discovery of novel bioactive molecules, including compounds with potential to modulate rumen fermentation and inhibit methane emissions from enteric fermentation. These effects often result from metabolic shifts in the rumen fermentation that lead to increased production of propionate, but can also be caused by direct inhibition of methanogenic archaea or through a reduction in the production of substrates for methanogenesis. Culture-independent approaches based on genome mining and functional metagenomics demonstrated that the rumen is an underexplored resource for bioactive molecules, such as antimicrobial peptides, non-ribosomal peptides, polyketides, and secondary metabolites involved in intercellular (microbe-microbe) communication. Rumen metatranscriptomic data indicate that the expression of genes potentially encoding some of these molecules is increased during the colonization of plant biomass that enters the rumen. Nonetheless, some representatives of key taxa from the core rumen microbiome cannot be found in culture collections, which is critical to validate phenotypic predictions from genomic and metagenomic data and obtain ecological insights about the interplay between individual microbial populations in the microbiome. Culturomic technologies and high-throughput identification and characterization of microbial species that colonize the rumen could contribute to building a unique biotechnological resource that can be explored for sourcing novel bioactive compounds with anti-methanogenic activity and developing fermentation products that could reduce rumen methanogenesis while improving the health status and productivity of cattle.