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Journal articles on the topic 'S. paucimobilis'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Ionescu, Mihaela Ileana, Dan Ștefan Neagoe, Alexandra Marioara Crăciun, and Oana Teodora Moldovan. "The Gram-Negative Bacilli Isolated from Caves—Sphingomonas paucimobilis and Hafnia alvei and a Review of Their Involvement in Human Infections." International Journal of Environmental Research and Public Health 19, no. 4 (2022): 2324. http://dx.doi.org/10.3390/ijerph19042324.

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The opportunistic infections with Gram-negative bacilli are frequently reported. The clinical studies are focused on the course of human infectious and very often the source of infection remain unclear. We aim to see if the Gram-negative bacilli isolated from a non-contaminated environment—the caves—are reported in human infections. Eleven samples were collected from six Romanian caves. We used the standard procedure used in our clinical laboratory for bacterial identification and for antibiotic susceptibility testing of the cave isolates. Out of the 14 bacterial strains, three isolates are Gr

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2

Marincu, Iosif, Felix Bratosin, Iulia Bogdan, et al. "Concurrent Sphingomonas paucimobilis and Mycobacterium tuberculosis Meningitis in an Immunocompromised Patient: A Rare Case Report and Comprehensive Review of Literature." Medicina 59, no. 4 (2023): 687. http://dx.doi.org/10.3390/medicina59040687.

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Sphingomonas paucimobilis is a gram-negative bacillus that is widely distributed in the environment but rarely causes infections in humans. Meningitis caused by S. paucimobilis is an extremely rare clinical entity with very few reported cases in the literature. The clinical presentation and management of S. paucimobilis meningitis are not well established, and further research is needed to better understand this rare infection. Therefore, the goal of this study was to present probably the only case of meningitis caused by co-infection with S. paucimobilis and Mycobacterium tuberculosis and to

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3

Yilmaz, Busra Zeynep, Özge Metin Akcan, Sevgi Pekcan, Havva Parlatan, Ganime Dilek Emlik, and Metin Doğan. "A Rare Presentation of Sphingomonas paucimobilis in a Healthy Child: Acute Ischemic Stroke." Journal of Pediatric Infectious Diseases 18, no. 03 (2023): 168–70. http://dx.doi.org/10.1055/s-0043-1767770.

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Abstract Sphingomonas paucimobilis is a rarely pathogenic organism that usually infects immunocompromised patients and causes nosocomial infections, but a few community-acquired infections have been identified in relatively healthy adult patients. Herein we report a 5-year-old child who presented with acute ischemic stroke caused by S. paucimobilis that was treated successfully with antibiotics and antithrombotic agents. According to our knowledge, this is the first case of acute ischemic infarction with S. paucimobilis in a previously healthy child.

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4

Rinawati, Weny, and July Kumalawati. "Sphingomonas paucimobilis infection in subcutaneous abdominal preservation of bone flap after craniotomy." Journal of Infection in Developing Countries 16, no. 02 (2022): 388–91. http://dx.doi.org/10.3855/jidc.15369.

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Introduction: Sphingomonas paucimobilis, previously known as Pseudomonas paucimobilis, is a Gram-negative rod. It is emerging as an opportunistic pathogen that can infect individuals in community or hospital settings. It is believed that the natural habitat of this organism is soil and water, including water sources in the hospital environment. Case report: We describe the case of a 46-year-old patient in whom S. paucimobilis was identified in the implanted bone flap after craniotomy. The postoperative bone flap was implanted in the right hypochondria and replaced after 8 weeks. There wa

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5

Kuo, I.-Ching, Po-Liang Lu, Wei-Ru Lin, et al. "Sphingomonas paucimobilis bacteraemia and septic arthritis in a diabetic patient presenting with septic pulmonary emboli." Journal of Medical Microbiology 58, no. 9 (2009): 1259–63. http://dx.doi.org/10.1099/jmm.0.009985-0.

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Sphingomonas paucimobilis, a yellow-pigmented, aerobic, glucose non-fermenting, Gram-negative bacillus, is a rare cause of human infection normally associated with immunocompromised hosts. We report a case of bacteraemia and septic arthritis in a 47-year-old diabetic man who presented with septic pulmonary emboli due to S. paucimobilis. The patient had an initial presentation of fever, right knee pain, coughing, dyspnoea and chest pain. The infection was treated successfully by surgical debridement combined with meropenem plus ciprofloxacin, based on the patient's antibiotic susceptibility pro

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6

Kumari, Rekha, Sanjukta Subudhi, Mrutyunjay Suar, et al. "Cloning and Characterization of lin Genes Responsible for the Degradation of Hexachlorocyclohexane Isomers by Sphingomonas paucimobilis Strain B90." Applied and Environmental Microbiology 68, no. 12 (2002): 6021–28. http://dx.doi.org/10.1128/aem.68.12.6021-6028.2002.

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ABSTRACT Hexachlorocyclohexane (HCH) has been used extensively against agricultural pests and in public health programs for the control of mosquitoes. Commercial formulations of HCH consist of a mixture of four isomers, α, β, γ, and δ. While all these isomers pose serious environmental problems, β-HCH is more problematic due to its longer persistence in the environment. We have studied the degradation of HCH isomers by Sphingomonas paucimobilis strain B90 and characterized the lin genes encoding enzymes from strain B90 responsible for the degradation of HCH isomers. Two nonidentical copies of

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7

Hussein, Nadheema Hammood, Israa M. S. AL-Kadmy, Suhad Abbas Abid, et al. "Detection of NDM-1 harboring Moraxella lacunata and Sphingomonas paucimobilis: first report from Iraq." Reviews and Research in Medical Microbiology 35, no. 4 (2023): 212–17. http://dx.doi.org/10.1097/mrm.0000000000000362.

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Background and aim: This study aimed to detect New Delhi metallo-beta-lactamase 1 (NDM-1) harboring Moraxella lacunata and Sphingomonas paucimobilis for the first time in Iraq. Material and methods: One hundred and sixty-nine bacterial isolates were collected from blood samples in Baghdad children hospital. Two bacterial species based on the selection of carbapenem will be reported for this study. The results are confirmed by conventional PCR and detection about NDM-1 gene that responsible of carbapenemases resistance. Results: Three isolates (one M. lacunata and two S. paucimobilis) were resi

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8

Rognrud, Kara, Andrew M. Diaz, Collin Hill, and Melissa A. Kershaw. "Bacterial Endocarditis Caused by Sphingomonas paucimobilis: A Case Report and Literature Review." Case Reports in Infectious Diseases 2020 (October 10, 2020): 1–3. http://dx.doi.org/10.1155/2020/7185834.

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A 47-year-old male with no significant medical history was hospitalized for bacteremia and diagnosed with endocarditis. The organism isolated was a Gram-negative bacillus—Sphingomonas paucimobilis. There are only a few reported cases of endocarditis caused by S. paucimobilis, and to our knowledge, this is the first in the United States.

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9

Suar, Mrutyunjay, Andrea Hauser, Thomas Poiger та ін. "Enantioselective Transformation of α-Hexachlorocyclohexane by the Dehydrochlorinases LinA1 and LinA2 from the Soil Bacterium Sphingomonas paucimobilis B90A". Applied and Environmental Microbiology 71, № 12 (2005): 8514–18. http://dx.doi.org/10.1128/aem.71.12.8514-8518.2005.

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ABSTRACT Sphingomonas paucimobilis B90A contains two variants, LinA1 and LinA2, of a dehydrochlorinase that catalyzes the first and second steps in the metabolism of hexachlorocyclohexanes (R. Kumari, S. Subudhi, M. Suar, G. Dhingra, V. Raina, C. Dogra, S. Lal, J. R. van der Meer, C. Holliger, and R. Lal, Appl. Environ. Microbiol. 68:6021-6028, 2002). On the amino acid level, LinA1 and LinA2 were 88% identical to each other, and LinA2 was 100% identical to LinA of S. paucimobilis UT26. Incubation of chiral α-hexachlorocyclohexane (α-HCH) with Escherichia coli BL21 expressing functional LinA1 a

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10

Acharya, Manisha, Javed Hussain Farooqui, Hayden Spencer, Vinay Arora, and Umang Mathur. "Sphingomonas paucimobilis keratitis post cataract surgery: First case report from India." Nepalese Journal of Ophthalmology 11, no. 1 (2019): 82–85. http://dx.doi.org/10.3126/nepjoph.v11i1.25426.

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Sphingomonas paucimobilis is a rare, aerobic Gram-negative soil bacillus rarely associated with intraocular infections. With only 3 cases of ophthalmic manifestations reported so far, we are presenting the fi rst case of S. Paucimobilis, causing keratitisafter cataract surgery, from India. The organism, which was resistant to initial medical treatment, eventually responded to a patch graft and the patient improved to a final visual acuity of 20/40.

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11

Nada, S. Razuqi, S. Muftin Fatema, H. Murbat Hamid, and Kh. Abdalameer Nisreen. "Influence of Dielectric-barrier Discharge (DBD) Cold Plasma on Water Contaminated Bacteria." Annual Research & Review in Biology 14, no. 4 (2017): 1–9. https://doi.org/10.9734/ARRB/2017/34642.

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<strong>Background and Aim:</strong> Dielectric Barrier Discharge (DBD) cold plasma has efficient antibacterial activity on bacterial contamination at a short period of time and confirms its potential for routine used in clinical environment biodecontamination as an alternative to conventional disinfectant methods of water treatment. The goal of this study is to evaluated <em>in vitro</em> effectiveness of DBD cold plasma on clinically important opportunistic pathogens that identified from water samples which were: <em>Legionella</em> <em>E.meningosepteca</em> and <em>S. paucimobilis</em> grow

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12

Sofoudis, Chrisostomos. "Sphingomonas paucimobilis complicating vaginal fluid cultivation. A rare case." Cancer Research and Cellular Therapeutics 5, no. 1 (2021): 01–03. http://dx.doi.org/10.31579/2640-1053/071.

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Sphingomonas paucimobilis represents an aerobic Gram-negative bacillus that is gaining recognition as an important human pathogen. These species are widely distributed in both natural environment and hospitals. They appear as opportunistic pathogen that take advantage of underlying conditions and diseases. Regardless of the clinical significance, pathogenic mechanism varies throughout current bibliography. Aim of our study, reflects presentation of a rare case of an out-patient clinical asymptomatic, with vaginal culture positive for this rare microorganism, S. paucimobilis. Assiduous diagnosi

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13

Harrison, Peter J., Kenneth Gable, Niranjanakumari Somashekarappa, et al. "Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase." Journal of Lipid Research 60, no. 5 (2019): 953–62. http://dx.doi.org/10.1194/jlr.m089367.

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Isotope labels are frequently used tools to track metabolites through complex biochemical pathways and to discern the mechanisms of enzyme-catalyzed reactions. Isotopically labeled l-serine is often used to monitor the activity of the first enzyme in sphingolipid biosynthesis, serine palmitoyltransferase (SPT), as well as labeling downstream cellular metabolites. Intrigued by the effect that isotope labels may be having on SPT catalysis, we characterized the impact of different l-serine isotopologues on the catalytic activity of recombinant SPT isozymes from humans and the bacterium Sphingomon

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14

Gad, Hend A., Ahmed A. Tayel, Mohammed S. Al-Saggaf, Shaaban H. Moussa, and Amany M. Diab. "Phyto-fabrication of selenium nanorods using extract of pomegranate rind wastes and their potentialities for inhibiting fish-borne pathogens." Green Processing and Synthesis 10, no. 1 (2021): 529–37. http://dx.doi.org/10.1515/gps-2021-0049.

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Abstract The invasion of fish/seafoods by zoonotic pathogens causes health threats to humans. Plant derivatives and phytosynthesized nanometals could promisingly overcome bacterial infections/contaminations. The extract of pomegranate rinds (PRE) was innovatively employed for biosynthesizing selenium nanorods (Se-NRs). These agents were assessed as antibacterial candidates against diverse fish-borne pathogenic species (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium, and Sphingomonas paucimobilis). The PRE-synthesized Se-NRs, within 60 min of contact, we

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15

Davison, Annette D., Duncan A. Veal, Peter Karuso, and Daniel R. Jardine. "Halopicolinic acids, novel products arising through the degradation of chloro- and bromo-biphenyl bySphingomonas paucimobilisBPSI-3." Canadian Journal of Microbiology 42, no. 1 (1996): 66–71. http://dx.doi.org/10.1139/m96-009.

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Sphingomonas paucimobilis BPSI-3 was previously isolated from a mixed microbial consortium growing on biphenyl as the sole source of carbon and energy. Transformation of 4 -chlorobiphenyl (4CBP) was demonstrated by this strain, although little or no growth was observed. In minimal salts medium supplemented with 4CBP or bromobiphenyl and dextrose, yellow coloured product(s) were rapidly formed. Gas chromatography – mass spectrometry (GC–MS) revealed single ring N-heterocyclic compounds that were identified as halopicolinic acids. We believe this to be the first report of such compounds being fo

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16

Miyauchi, Keisuke, Seug-Kyo Suh, Yuji Nagata та Masamichi Takagi. "Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of γ-Hexachlorocyclohexane by Sphingomonas paucimobilis". Journal of Bacteriology 180, № 6 (1998): 1354–59. http://dx.doi.org/10.1128/jb.180.6.1354-1359.1998.

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ABSTRACT Sphingomonas (formerly Pseudomonas)paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed that γ-HCH is degraded to 2,5-dichlorohydroquinone (2,5-DCHQ) (Y. Nagata, R. Ohtomo, K. Miyauchi, M. Fukuda, K. Yano, and M. Takagi, J. Bacteriol. 176:3117–3125, 1994). In the present study, we cloned and characterized a gene, designated linD, directly involved in the degradation of 2,5-DCHQ. The linD gene encodes a peptide of 343 amino acids and has a low level of similarity to proteins whi

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17

Bayram, Nuri, İlker Devrim, Hurşit Apa, Gamze Gülfidan, Hande Namal Türkyılmaz, and İlker Günay. "SPHINGOMONAS PAUCIMOBILIS INFECTIONS IN CHILDREN: NOSOCOMIAL VERSUS COMMUNITY ACQUIRED INFECTIONS." Mediterranean Journal of Hematology and Infectious Diseases 5, no. 1 (2013): e2013040. http://dx.doi.org/10.4084/mjhid.2013.040.

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Sphingomonas paucimobilis is a causative agent of infection in immunocompromised patients, and healthcare-associated infections. Although the infections associated with S.paucimobilis occurs rarely, it has been encountered with increasing frequency in clinical settings. In the current study we noted the risk factors and clinical features of the children with S.paucimobilis infections, and the antimicrobial susceptibilities of the isolated strains among the patients. This study was conducted in Dr. Behçet Uz Children’s Hospital, Turkey, during the period of January 2005 and December 2012. The m

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18

MARQUES, Ana Rita, Pedro M. COUTINHO, Paula VIDEIRA, Arsénio M. FIALHO, and Isabel SÁ-CORREIA. "Sphingomonas paucimobilis beta-glucosidase Bgl1: a member of a new bacterial subfamily in glycoside hydrolase family 1." Biochemical Journal 370, no. 3 (2003): 793–804. http://dx.doi.org/10.1042/bj20021249.

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The Sphingomonas paucimobilis β-glucosidase Bgl1 is encoded by the bgl1 gene, associated with an 1308bp open reading frame. The deduced protein has a potential signal peptide of 24 amino acids in the N-terminal region, and experimental evidence is consistent with the processing and export of the Bgl1 protein through the inner membrane to the periplasmic space. A His6-tagged 44.3kDa protein was over-produced in the cytosol of Escherichia coli from a recombinant plasmid, which contained the S. paucimobilis bgl1 gene lacking the region encoding the putative signal peptide. Mature β-glucosidase Bg

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19

Annisa Muhyi and Amalia Aswin. "Sphingomonas paucimobilis an unusual cause of subdural empyema in pediatric: a case report." Pediatrics Sciences Journal 2, no. 1 (2022): 1–3. http://dx.doi.org/10.51559/pedscij.v2i1.19.

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Introduction: Acute bacterial meningitis continues to be a neurological emergency with high mortality and morbidity Case: We report the case of a pediatric patient with subdural empyema due to Sphingomonas paucimobilis. A 2-month-old infant developed fever, generalized seizures, and worsening general status. He had a cough for two weeks, and a chest X-ray showed pneumonia. Laboratory tests reveal marked leukocytosis, a white blood cell count of 28.500/mL, and an elevated platelet level of 654.000 mg/dL. A computerized brain tomography revealed an empyema subdural. A cerebrospinal fluid a

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VIDEIRA, Paula, Arsénio FIALHO, Roberto A. GEREMIA, Christelle BRETON та Isabel SÁ-CORREIA. "Biochemical characterization of the β-1,4-glucuronosyltransferase GelK in the gellan gum-producing strain Sphingomonas paucimobilis A.T.C.C. 31461". Biochemical Journal 358, № 2 (2001): 457–64. http://dx.doi.org/10.1042/bj3580457.

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Biosynthesis of bacterial polysaccharide-repeat units proceeds by sequential transfer of sugars, from the appropriate sugar donor to an activated lipid carrier, by committed glycosyltransferases (GTs). Few studies on the mechanism of action for this type of GT are available. Sphingomonas paucimobilis A.T.C.C. 31461 produces the industrially important polysaccharide gellan gum. We have cloned the gelK gene from S. paucimobilis A.T.C.C. 31461. GelK belongs to family 1 of the GT classification [Campbell, Davies, Bulone, Henrissat (1997) Biochem. J. 326, 929–939]. Sequence similarity studies sugge

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Naka, Takashi, Nagatoshi Fujiwara, Eiko Yabuuchi, et al. "A Novel Sphingoglycolipid Containing Galacturonic Acid and 2-Hydroxy Fatty Acid in Cellular Lipids ofSphingomonas yanoikuyae." Journal of Bacteriology 182, no. 9 (2000): 2660–63. http://dx.doi.org/10.1128/jb.182.9.2660-2663.2000.

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ABSTRACT A novel sphingoglycolipid was isolated from Sphingomonas yanoikuyae, and its structure was identified as a galacturonosyl-β (1→1)-ceramide. This was a characteristic sphingoglycolipid present in S. yanoikuyae and certain other species of Sphingomonas, such as Sphingomonas mali, Sphingomonas terrae, and Sphingomonas macrogoltabidus, but not in the type species ofSphingomonas, Sphingomonas paucimobilis.

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22

Faletrov, Y. V., V. O. Maliugin, N. S. Frolova, and V. M. Shkumatov. "<i>In silico</i> evaluation of new affine interactions of methylcoumarin with cytochromes P450." Proceedings of the National Academy of Sciences of Belarus, Chemical Series 58, no. 2 (2022): 186–90. http://dx.doi.org/10.29235/1561-8331-2022-58-2-186-190.

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4-methyl-7-methoxycoumarin (CumOMe) has been synthesized and in silico calculations demonstrated localization of methoxy group within 0.4 nm from Fe ion of hem groups for some structures of human CYP19 & CYP46 as well as CYP152 S. paucimobilis, CYP158 St. coelicolor, HMUO C. diphtheriae, XPLA R. rhodochrous, CYP199A4 Rh. palustris, CYP101A1 Ps. putida and CYP51 M. tuberculosis.

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Dogra, Charu, Vishakha Raina, Rinku Pal, et al. "Organization of lin Genes and IS6100 among Different Strains of Hexachlorocyclohexane-Degrading Sphingomonas paucimobilis: Evidence for Horizontal Gene Transfer." Journal of Bacteriology 186, no. 8 (2004): 2225–35. http://dx.doi.org/10.1128/jb.186.8.2225-2235.2004.

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ABSTRACT The organization of lin genes and IS6100 was studied in three strains of Sphingomonas paucimobilis (B90A, Sp+, and UT26) which degraded hexachlorocyclohexane (HCH) isomers but which had been isolated at different geographical locations. DNA-DNA hybridization data revealed that most of the lin genes in these strains were associated with IS6100, an insertion sequence classified in the IS6 family and initially found in Mycobacterium fortuitum. Eleven, six, and five copies of IS6100 were detected in B90A, Sp+, and UT26, respectively. IS6100 elements in B90A were sequenced from five, one,

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Hara, Hirofumi, Eiji Masai, Keisuke Miyauchi, Yoshihiro Katayama, and Masao Fukuda. "Characterization of the 4-Carboxy-4-Hydroxy-2-Oxoadipate Aldolase Gene and Operon Structure of the Protocatechuate 4,5-Cleavage Pathway Genes in Sphingomonas paucimobilis SYK-6." Journal of Bacteriology 185, no. 1 (2003): 41–50. http://dx.doi.org/10.1128/jb.185.1.41-50.2003.

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ABSTRACT The protocatechuate (PCA) 4,5-cleavage pathway is the essential metabolic route for degradation of low-molecular-weight products derived from lignin by Sphingomonas paucimobilis SYK-6. In the 10.5-kb EcoRI fragment carrying the genes for PCA 4,5-dioxygenase (ligAB), 2-pyrone-4,6-dicarboxylate hydrolase (ligI), 4-oxalomesaconate hydratase (ligJ), and a part of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (ligC), we found the ligK gene, which encodes 4-carboxy-4-hydroxy-2-oxoadipate (CHA) aldolase. The ligK gene was located 1,183 bp upstream of ligI and transcribed in the sa

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Leaphart, A. B., M. J. Friez, and C. R. Lovell. "Formyltetrahydrofolate Synthetase Sequences from Salt Marsh Plant Roots Reveal a Diversity of Acetogenic Bacteria and Other Bacterial Functional Groups." Applied and Environmental Microbiology 69, no. 1 (2003): 693–96. http://dx.doi.org/10.1128/aem.69.1.693-696.2003.

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ABSTRACT Sixty-two partial formyltetrahydrofolate synthetase (FTHFS) structural gene sequences were recovered from roots of salt marsh plants, including Spartina alterniflora, Salicornia virginica, and Juncus roemerianus. Only S. alterniflora roots yielded sequences grouping with FTHFS sequences from known acetogens. Most other FTHFS or FTHFS-like sequences grouped with those from sulfate-reducing bacteria. Several sequences that grouped with Sphingomonas paucimobilis ligH were also recovered.

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Sonoki, Tomonori, Takahiro Obi, Sachiko Kubota, Motoo Higashi, Eiji Masai, and Yoshihiro Katayama. "Coexistence of Two Different O Demethylation Systems in Lignin Metabolism by Sphingomonas paucimobilis SYK-6: Cloning and Sequencing of the Lignin Biphenyl-Specific O-Demethylase (LigX) Gene." Applied and Environmental Microbiology 66, no. 5 (2000): 2125–32. http://dx.doi.org/10.1128/aem.66.5.2125-2132.2000.

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ABSTRACT Sphingomonas paucimobilis SYK-6 can grow on several dimeric model compounds of lignin as a carbon and energy source. It has O demethylation systems on three kinds of substrates: 5,5′-dehydrodivanillic acid (DDVA), syringate, and vanillate. We previously reported the cloning of a gene involved in the tetrahydrofolate-dependent O demethylation of syringate and vanillate. In the study reported here, we cloned the gene responsible for DDVA O demethylation. Using nitrosoguanidine mutagenesis, a mutant strain, NT-1, which could not degrade DDVA but could degrade syringate and vanillate, was

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Titah, Harmin Sulistiyaning, Siti Rozaimah Sheikh Abdullah, Mushrifah Idris, et al. "Arsenic Resistance and Biosorption by Isolated Rhizobacteria from the Roots of Ludwigia octovalvis." International Journal of Microbiology 2018 (December 30, 2018): 1–10. http://dx.doi.org/10.1155/2018/3101498.

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Certain rhizobacteria can be applied to remove arsenic in the environment through bioremediation or phytoremediation. This study determines the minimum inhibitory concentration (MIC) of arsenic on identified rhizobacteria that were isolated from the roots of Ludwigia octovalvis (Jacq.) Raven. The arsenic biosorption capability of the was also analyzed. Among the 10 isolated rhizobacteria, five were Gram-positive (Arthrobacter globiformis, Bacillus megaterium, Bacillus cereus, Bacillus pumilus, and Staphylococcus lentus), and five were Gram-negative (Enterobacter asburiae, Sphingomonas paucimob

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Nishikawa, Seiji, Tomonori Sonoki, Tatsuhide Kasahara, et al. "Cloning and Sequencing of theSphingomonas (Pseudomonas)paucimobilis Gene Essential for the O Demethylation of Vanillate and Syringate." Applied and Environmental Microbiology 64, no. 3 (1998): 836–42. http://dx.doi.org/10.1128/aem.64.3.836-842.1998.

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ABSTRACT Sphingomonas (Pseudomonas)paucimobilis SYK-6 is able to grow on 5,5′-dehydrodivanillic acid (DDVA), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. Nitrosoguanidine mutagenesis of S. paucimobilis SYK-6 was performed, and two mutants with altered DDVA degradation pathways were isolated. The mutant strain NT-1 could not degrade DDVA, but could degrade syringate, vanillate, and 2,2′,3′-trihydroxy-3-methoxy-5,5′-dicarboxybiphenyl (OH-DDVA). Strain DC-49 could slowly assimilate DDVA, but could degrade neither vanillate nor syringate, although it c

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Raj, M. Shailaja, I. Furtado та S. Mavinkurve. "Periplasmically located α-santonin binding factor in Sphingomonas paucimobilis strain S ATCC 43388". Indian Journal of Microbiology 47, № 4 (2007): 358–63. http://dx.doi.org/10.1007/s12088-007-0064-0.

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Fialho, Arsénio M., Lígia O. Martins, Marie-Lucie Donval, et al. "Structures and Properties of Gellan Polymers Produced bySphingomonas paucimobilis ATCC 31461 from Lactose Compared with Those Produced from Glucose and from Cheese Whey." Applied and Environmental Microbiology 65, no. 6 (1999): 2485–91. http://dx.doi.org/10.1128/aem.65.6.2485-2491.1999.

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ABSTRACT The dairy industry produces large quantities of whey as a by-product of cheese production and is increasingly looking for new ways to utilize this waste product. Gellan gum is reliably produced bySphingomonas paucimobilis in growth media containing lactose, a significant component of cheese whey, as a carbon source. We studied and compared polysaccharide biosynthesis by S. paucimobilis ATCC 31461 in media containing glucose, lactose (5 to 30 g/liter), and sweet cheese whey. We found that altering the growth medium can markedly affect the polysaccharide yield, acyl substitution level,

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Suar, Mrutyunjay, Jan Roelof van der Meer, Kirsten Lawlor, Christof Holliger, and Rup Lal. "Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers." Applied and Environmental Microbiology 70, no. 11 (2004): 6650–56. http://dx.doi.org/10.1128/aem.70.11.6650-6656.2004.

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ABSTRACT Sphingomonas paucimobilis B90A is able to degrade the α-, β-, γ-, and δ-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown S. paucimobilis B90A by RNA dot blot hybridization and real-time reverse transcriptase PCR upon exposure to a pulse of different HCH isomers. Irrespective of the addition of HCH, linA, linB, and linC were all expressed constitutively. In contrast, linD and linE were induced with α-HCH (

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Kim, Woo, Joo Park, Jae Park, Doo Choi, and Yong Park. "Purification and Characterization of a Fucoidanase (FNase S) from a Marine Bacterium Sphingomonas paucimobilis PF-1." Marine Drugs 13, no. 7 (2015): 4398–417. http://dx.doi.org/10.3390/md13074398.

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Thompson, Alex F., Erika L. English, Adam M. Nock, et al. "Characterizing species interactions that contribute to biofilm formation in a multispecies model of a potable water bacterial community." Microbiology 166, no. 1 (2020): 34–43. http://dx.doi.org/10.1099/mic.0.000849.

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Microbial biofilms are ubiquitous in drinking water systems, yet our understanding of drinking water biofilms lags behind our understanding of those in other environments. Here, a six-member model bacterial community was used to identify the interactions and individual contributions of each species to community biofilm formation. These bacteria were isolated from the International Space Station potable water system and include Cupriavidus metallidurans , Chryseobacterium gleum , Ralstonia insidiosa, Ralstonia pickettii, Methylorubrum (Methylobacterium) populi and Sphingomonas paucimobilis , bu

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Klopfleisch, R., U. Polster, K. Klingler, and J. P. Teifke. "Hochgradige, aber klinisch »stumme« Cholangiolithiasis beim Rind." Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere 32, no. 01 (2004): 13–17. http://dx.doi.org/10.1055/s-0038-1623280.

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ZusammenfassungBerichte über Konkremente der Gallenwege bei Haustieren sind selten, nicht zuletzt deshalb, weil die Cholelithiasis beim Tier meist klinisch inapparent verläuft. Bei Rindern können Gallensteine aber gelegentlich zu Kolikerscheinungen mit Diarrhö führen. Gallenpigmentsteine und Gallenpigment- Kalksteine stellen bei dieser Tierart den häufigsten Steintyp dar. Der vorliegende Beitrag berichtet über eine massive, jedoch klinisch inapparente Cholelithiasis bei einem 17-jährigen, weiblichen Niederungsrind. Bei der Sektion des mit dem bovinen Leukosevirus infizierten Tieres fand sich e

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Safari, Wulan Fitriani, Sri Wardhiati, and Almatin Puspa Dewi. "Identifikasi Bakteri Dari Sampel Darah Pasien COVID-19 Di RSUD Ciawi Kabupaten Bogor." Journal of Medical Laboratory Research 2, no. 2 (2024): 12–17. http://dx.doi.org/10.36743/jomlr.v2i2.712.

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Coronavirus Disease 2019 (COVID-19) is a disease caused by the Severe Acute Respiratory Syndrome Corona Virus 2 virus called SARSCov-2, this virus attacks the respiratory tract and creates a risk of infection in COVID-19 patients. Bacterial infections are known to complicate viral infections of the respiratory tract and are associated with worsening conditions in COVID-19 patients. The aim of this research is to identify bacteria from blood samples of COVID-19 patients at Ciawi Hospital, Bogor Regency. This type of research is quantitative descriptive which describes the types of bacteria from

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Kim, Yeon-Ju, Joon Young Park, Sri Renukadevi Balusamy, et al. "Comprehensive Genome Analysis on the Novel Species Sphingomonas panacis DCY99T Reveals Insights into Iron Tolerance of Ginseng." International Journal of Molecular Sciences 21, no. 6 (2020): 2019. http://dx.doi.org/10.3390/ijms21062019.

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Plant growth-promoting rhizobacteria play vital roles not only in plant growth, but also in reducing biotic/abiotic stress. Sphingomonas panacis DCY99T is isolated from soil and root of Panax ginseng with rusty root disease, characterized by raised reddish-brown root and this is seriously affects ginseng cultivation. To investigate the relationship between 159 sequenced Sphingomonas strains, pan-genome analysis was carried out, which suggested genomic diversity of the Sphingomonas genus. Comparative analysis of S. panacis DCY99T with Sphingomonas sp. LK11 revealed plant growth-promoting potent

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Masai, Eiji, Shouji Shinohara, Hirofumi Hara, Seiji Nishikawa, Yoshihiro Katayama, and Masao Fukuda. "Genetic and Biochemical Characterization of a 2-Pyrone-4,6-Dicarboxylic Acid Hydrolase Involved in the Protocatechuate 4,5-Cleavage Pathway of Sphingomonas paucimobilis SYK-6." Journal of Bacteriology 181, no. 1 (1999): 55–62. http://dx.doi.org/10.1128/jb.181.1.55-62.1999.

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ABSTRACT Sphingomonas paucimobilis SYK-6 is able to grow on a wide variety of dimeric lignin compounds with guaiacyl moieties, which are converted into protocatechuate by the actions of lignin degradation enzymes in this strain. Protocatechuate is a key metabolite in the SYK-6 degradation of lignin compounds with guaiacyl moieties, and it is thought that it degrades to pyruvate and oxaloacetate via the protocatechuate 4,5-cleavage pathway. In a 10.5-kb EcoRI fragment carrying the protocatechuate 4,5-dioxygenase gene (ligAB) (Y. Noda, S. Nishikawa, K. Shiozuka, H. Kadokura, H. Nakajima, K. Yoda

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Rajendrasozhan, Saravanan, Hani El Moll, Mejdi Snoussi, et al. "Phytochemical Screening and Antimicrobial Activity of Various Extracts of Aerial Parts of Rhanterium epapposum." Processes 9, no. 8 (2021): 1351. http://dx.doi.org/10.3390/pr9081351.

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Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used to cure skin infections. The objective is to screen the phytochemical content and antimicrobial activity of aqueous, methanol and 80% methanol extracts of aerial parts of R. epapposum. The phytochemical screening of aqueous, methanolic, and 80% methanol extracts of R. epapposum was conducted using gas chromatographymass spectrometry. The antimicrobial activities of the extracts were assessed by well diffusion and microdilution methods. Qualitative phytochemical analysis revealed the presence of 2-methoxy-4-vinylphenol

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Yang, Suijuan, Xinghai Zhang, Zhaoyun Cao, et al. "Growth‐promoting S phingomonas paucimobilis ZJSH 1 associated with D endrobium officinale through phytohormone production and nitrogen fixation." Microbial Biotechnology 7, no. 6 (2014): 611–20. http://dx.doi.org/10.1111/1751-7915.12148.

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Blachowicz, Adriana, Camilla Urbaniak, Alec Adolphson, Gwyn Isenhouer, Andy Page, and Kasthuri Venkateswaran. "Microbial Detection and Quantification of Low-Biomass Water Samples Using an International Space Station Smart Sample Concentrator." Microorganisms 11, no. 9 (2023): 2310. http://dx.doi.org/10.3390/microorganisms11092310.

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The pressing need to safeguard the health of astronauts aboard the International Space Station (ISS) necessitates constant and rigorous microbial monitoring. Recognizing the shortcomings of traditional culture-based methods, NASA is deliberating the incorporation of molecular-based techniques. The challenge, however, lies in developing and validating effective methods for concentrating samples to facilitate this transition. This study is dedicated to investigating the potential of an ISS Smart Sample Concentrator (iSSC) as an innovative concentration method. First, the iSSC system and its comp

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Masai, Eiji, Miyuki Sasaki, Yasunori Minakawa, et al. "A Novel Tetrahydrofolate-Dependent O-Demethylase Gene Is Essential for Growth of Sphingomonas paucimobilis SYK-6 with Syringate." Journal of Bacteriology 186, no. 9 (2004): 2757–65. http://dx.doi.org/10.1128/jb.186.9.2757-2765.2004.

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ABSTRACT Sphingomonas paucimobilis SYK-6 degrades syringate to 3-O-methylgallate (3MGA), which is finally converted to pyruvate and oxaloacetate via multiple pathways in which protocatechuate 4,5-dioxygenase, 3MGA dioxygenase, and gallate dioxygenase are involved. Here we isolated the syringate O-demethylase gene (desA), which complemented the growth deficiency on syringate of a Tn5 mutant of the SYK-6 derivative strain. The desA gene is located 929 bp downstream of ferA, encoding feruloyl-coenzyme A synthetase, and consists of a 1,386-bp open reading frame encoding a polypeptide with a molecu

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Masai, Eiji, Atsushi Ichimura, Yusuke Sato, Keisuke Miyauchi, Yoshihiro Katayama та Masao Fukuda. "Roles of the Enantioselective Glutathione S-Transferases in Cleavage of β-Aryl Ether". Journal of Bacteriology 185, № 6 (2003): 1768–75. http://dx.doi.org/10.1128/jb.185.6.1768-1775.2003.

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ABSTRACT Cleavage of the β-aryl ether linkage is the most important process in lignin degradation. Here we characterize the three tandemly located glutathione S-transferase (GST) genes, ligF, ligE, and ligG, from low-molecular-weight lignin-degrading Sphingomonas paucimobilis SYK-6, and we describe the actual roles of these genes in the β-aryl ether cleavage. Based on the identification of the reaction product by electrospray ionization-mass spectrometry, a model compound of β-aryl ether, α-(2-methoxyphenoxy)-β-hydroxypropiovanillone (MPHPV), was transformed by LigF or LigE to guaiacol and α-g

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Barkay, T., S. Navon-Venezia, E. Z. Ron, and E. Rosenberg. "Enhancement of Solubilization and Biodegradation of Polyaromatic Hydrocarbons by the Bioemulsifier Alasan." Applied and Environmental Microbiology 65, no. 6 (1999): 2697–702. http://dx.doi.org/10.1128/aem.65.6.2697-2702.1999.

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ABSTRACT Alasan, a high-molecular-weight bioemulsifier complex of an anionic polysaccharide and proteins that is produced by Acinetobacter radioresistens KA53 (S. Navon-Venezia, Z. Zosim, A. Gottlieb, R. Legmann, S. Carmeli, E. Z. Ron, and E. Rosenberg, Appl. Environ. Microbiol. 61:3240–3244, 1995), enhanced the aqueous solubility and biodegradation rates of polyaromatic hydrocarbons (PAHs). In the presence of 500 μg of alasan ml−1, the apparent aqueous solubilities of phenanthrene, fluoranthene, and pyrene were increased 6.6-, 25.7-, and 19.8-fold, respectively. Physicochemical characterizati

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Chen, Ying, Xinlin Wu, Yaming Zhao, Shaoning Chen, and Xiufang Hu. "Screening of Cadmium-Resistant Microbes to Improve the Tolerance of Sorghum bicolor (L.) Moench Against Cadmium." Journal of Biobased Materials and Bioenergy 17, no. 6 (2023): 716–24. http://dx.doi.org/10.1166/jbmb.2023.2321.

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To improve the cadmium (Cd) resistance and the remediation ability of Sorghum bicolor (L.) Moench, fourteen Cd-resistant bacteria were screened from cadmium-contaminated soil, together with two strains preserved in the laboratory. It was found that 3 strains (ZR451, ZR452, and ZJSH1) significantly promoted the growth of Cd-stressed S. bicolor in hydroponics and mitigated Cd toxicity to plants, and SR8, ZRK1, and ZR211 promoted sorghum growth lightly. Further studies indicated that strains SR8, ZR211, and ZJSH1 were Cd-resistant and acid-resistant strains. Strains ZR211and ZR121 significantly p

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Masai, Eiji, Kyo Harada, Xue Peng, Hirotaka Kitayama, Yoshihiro Katayama, and Masao Fukuda. "Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6." Applied and Environmental Microbiology 68, no. 9 (2002): 4416–24. http://dx.doi.org/10.1128/aem.68.9.4416-4424.2002.

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ABSTRACT Sphingomonas paucimobilis SYK-6 degrades ferulic acid to vanillin, and it is further metabolized through the protocatechuate 4,5-cleavage pathway. We obtained a Tn5 mutant of SYK-6, FA2, which was able to grow on vanillic acid but not on ferulic acid. A cosmid which complemented the growth deficiency of FA2 on ferulic acid was isolated. The 5.2-kb BamHI-EcoRI fragment in this cosmid conferred the transformation activity of ferulic acid to vanillin on Escherichia coli host cells. A sequencing analysis revealed the genes ferB and ferA in this fragment; these genes consist of 852- and 2,

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Stachon, Henrique, Vanessa Amoroso, Cicero Urban, et al. "Intraoperative Assessment of Endogenous Microbiota in the Breast." Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics 43, no. 10 (2021): 759–64. http://dx.doi.org/10.1055/s-0041-1736300.

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Abstract Objective: Breast surgery is considered a clean surgery; however, the rates of infection range between 3 and 15%. The objective of the present study was to intraoperatively investigate the presence of autochthonous microbiota in the breast. Methods: Pieces of breast tissue collected from 49 patients who underwent elective breast surgery (reconstructive, diagnostic, or oncologic) were cultured. The pieces of breast tissue were approximately 1 cm in diameter and were removed from the retroareolar area, medial quadrant, and lateral quadrant. Each piece of tissue was incubated in brain he

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Imbeault, Nathalie, Marcel Paquet, and Raynald Côté. "A Study of ATP and Biodegradation as a Measure of Bacterial Activity in an Anaerobic Fluidized Bed Bioreacto." Water Quality Research Journal 33, no. 3 (1998): 403–16. http://dx.doi.org/10.2166/wqrj.1998.023.

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Abstract In this study, the formation of the biofilm and the temporal change in the bacterial composition at different whey permeate (wastewater) sugar concentrations were assessed inside a fluidized bed bioreactor. At a sugar concentration of 5 g/L, the efficiency of biodégradation was 10%, while at 20 g/L it was 3%. With time, an increase in the ATP concentration of the bacteria on the coal grains was observed, indicating biofilm formation. To estimate the biodegradation activity in the biofilm, expressed as mass of sugar biodegraded per day and per volume of support, the bacterial ATP was d

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Kushnir, I. M., I. S. Semen, G. V. Kolodiy, S. D. Murska, V. I. Kushnir, and U. Z. Berbeka. "MICROBIOLOGICAL MONITORING OF HONEY." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 22, no. 1 (2021): 131–36. http://dx.doi.org/10.36359/scivp.2021-22-1.15.

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The article presents the results of determination the microbiological parameters of honey as one of the important factors which influence on the quality and safety of food.. Therefore. It was determined the quantitative and qualitative composition of honey microflora from different regions of Ukraine and also was studied the sensitivity of isolated cultures to antibiotics. The main microbiological risks are the increased level of mesophilic anaerobic facultative anaerobic microorganisms, contamination of honey with pathogenic and opportunistic microorganisms (Coliforms, Staphylococcus aureus,

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NISHIYAMA, M. "Identification of soil micro-habitats for growth, death and survival of a bacterium, ?-1,2,3,4,5,6-hexachlorocyclohexane-assimilating Sphingomonas paucimobilis, by fractionation of soil." FEMS Microbiology Reviews 101, no. 3 (1992): 145–50. http://dx.doi.org/10.1016/0168-6445(92)90010-s.

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Peng, Xue, Takashi Egashira, Kaoru Hanashiro, et al. "Cloning of a Sphingomonas paucimobilis SYK-6 Gene Encoding a Novel Oxygenase That Cleaves Lignin-Related Biphenyl and Characterization of the Enzyme." Applied and Environmental Microbiology 64, no. 7 (1998): 2520–27. http://dx.doi.org/10.1128/aem.64.7.2520-2527.1998.

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ABSTRACT Sphingomonas paucimobilis SYK-6 transforms 2,2′-dihydroxy-3,3′-dimethoxy-5,5′-dicarboxybiphenyl (DDVA), a lignin-related biphenyl compound, to 5-carboxyvanillic acid via 2,2′,3-trihydroxy-3′-methoxy-5,5′-dicarboxybiphenyl (OH-DDVA) as an intermediate (15). The ring fission of OH-DDVA is an essential step in the DDVA degradative pathway. A 15-kbEcoRI fragment isolated from the cosmid library complemented the growth deficiency of a mutant on OH-DDVA. Subcloning and deletion analysis showed that a 1.4-kb DNA fragment included the gene responsible for the ring fission of OH-DDVA. An open

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