Relevant bibliographies by topics / Sabouraud Dextrose Agar

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Academic literature on the topic 'Sabouraud Dextrose Agar'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Sabouraud Dextrose Agar"

1

Bulajic, Nina, Dragana Ivanovic, and Milan Savic. "Modified Sabouraud dextrose agar for isolation and identification of dermatophytes." Zbornik Matice srpske za prirodne nauke, no. 108 (2005): 291–99. http://dx.doi.org/10.2298/zmspn0508291b.

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The most common causative agents of dermatomycoses are fungi belonging to genders Trichophyton, Microsporum and Epidermophyton. Media mainly used for isolation of dermatophytes are mycobiotic agar, dermatophyte test medium Sabouraud agar (original formula or modification by Emmons) with or without antibiotics and cycloheximide. Peptones are the most important components of the media, which enable adequate reproductivity in identification of dermatophytes. Standard medium for isolation of dermatophytes is not produced in our country. The aim of the study was to create an optimal easily accessible and economic medium which enables isolation and identification of dermatophytes according to criteria for morphological diagnosis provided by identification guides. We examined 57 strains of Trichophyton, 24 of Microsporum and 5 of Epidermophyton floccosum (E. floccosum). Each strain was seeded on Sabouraud dextrose agar (Torlak Serbia and Montenegro), Sabouraud maltose agar (Torlak), two experimental modified Sabouraud dextrose agar media marked as SA-2 and SA-3 (Torlak) Sabouraud-Chloramp- henicole agar (Biomerieux, France) Sabouraud-Chloramphenicole agar (Himedia, India), Glucose-peptone agar (Himedia, India) and Sabouraud Emmons dextrose Agar with Chloramphenicole and Cycloheximide (Biolife, Italy). Colony morphology of Trichophyton mentagrophytes (T. mentagrophytes) was uni- form on all the media, while morphology of Trichophyton rubrum (T. rubrum) and Microsporum canis (M. canis) depended more on the media type. Colonies of E. floccosum were typical and uniform on all the media, as were the control species of Trichophyton schoenleinii (T. schoenleinii) and Trichophyton soudanense (T. soudanense). Experimental modified Sabouraud dextrose agar (Torlak) marked as SA-3 demonstrated the best results in identification of dermatophytes in this study.
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Wiwanitkit, Viroj. "Sabouraud dextrose agar for the diagnosis of fungal keratitis." Indian Journal of Ophthalmology 58, no. 6 (2010): 549. http://dx.doi.org/10.4103/0301-4738.71684.

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3

Sanjaya, Eko Muri, M. Cyccu Tobing, and Lisnawati Lisnawati. "Toksisitas Metabolit Skunder Penicillium Sp. Pada Berbagai Mediakultur Untuk Mengendalikan Spodoptera Sp. In Vitro." Talenta Conference Series: Agricultural and Natural Resources (ANR) 1, no. 1 (October 16, 2018): 131–37. http://dx.doi.org/10.32734/anr.v1i1.132.

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Penelitian untuk mengetahui toksisitas metabolit skunder Penicillium sp. pada berbagai media kultur yang akan digunakanuntuk mengendalikan Spodoptera sp. in vitro telah dilakukandi Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Sumatera Utara pada bulan Januari – Agustus 2016. Berbagai media biakan Penicillium sp. Yaitu Potato Dextrose Agar (Kontrol), Sabouraud Dextrose Agar, D0C2, Czapek Dox Agar, dan tepung yang dibuat dari tubuh Spodoptera sp. telah digunakan. Hasil penelitian menunjukkan media tepung yang dibuat dari tubuh Spodoptera sp. memiliki toksisitas tertinggi dengan persentase mortalitas 100% pada pengamatan jam ke enam. Lt50 didapat pada 3,545 jam. This study was to determine the toxicity of secondary metabolites of Penicillium sp. on various culture media used to control Spodoptera sp. in vitro. Various culture media for Penicillium sp. such as Potato Dextrose Agar (Control), Sabouraud Dextrose Agar, D0C2, Czapek Dox Agar, and flour made from the body of Spodoptera sp. has been used. The results showed that flour media made from the body of Spodoptera sp. has the highest toxicity with a percentage of 100% mortality at the sixth-hour observation. Lt50 is obtained at 3,545 hours.
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Guinea, Jesús, Teresa Peláez, Luis Alcalá, and Emilio Bouza. "Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia." Diagnostic Microbiology and Infectious Disease 53, no. 4 (December 2005): 333–34. http://dx.doi.org/10.1016/j.diagmicrobio.2005.07.002.

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Akgül, Öncü, and Nilgün Çerikçioğlu. "Hypertonic Sabouraud Dextrose Agar as a Substrate for Differentiation of Candida dubliniensis." Mycopathologia 167, no. 6 (February 20, 2009): 357–59. http://dx.doi.org/10.1007/s11046-009-9187-7.

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Kinash, O. V., and T. A. Skotarenko. "CULTURAL PROPERTIES OF FUNGI OF THE FAMILY MUCORACEAE, THE GENUS ASPERGILLUS AS CAUSATIVE AGENTS OF ZOOANTHROPONOSIS." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 19, no. 1 (April 26, 2019): 61–65. http://dx.doi.org/10.31718/2077-1096.19.1.61.

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At present, in Ukraine there is a growing prevalence of fungal infections, including aspergillosis and mucormycosis, against HIV / AIDS, tuberculosis, bronchial asthma and cancerous diseases [11]. The literature available presents scanty data on which of the most commonly used nutrient media provide the highest biosynthetic activity for the fungi of the family Mucoraceae, the genus Aspergillus. The aim of this study was to investigate the cultural properties of the studied isolates of fungi on different nutrient media. The following field fungi isolates were studied: Mucor ramosissimus Samutsevitsch, Rhizopus spp., Aspergillus fumigatus Fresenius, Aspergillus niger van Tieghem, Aspergillus flavus Link. The following nutrient media were used: the Plout medium, the Grigoraki medium, the Czapek agar, the Sabouraud agar, the wort-agar, the Van Eterson medium, the Sabouraud-dextrose broth, the honey medium. The concentration of fungi spores per 1 cm3 of inoculum was assessed on the basis of standard methods using a Goryaev's chamber. The evaluation of the intensity of spore formation on different nutrient media was carried out by standard techniques and assessed in CFU/ cm2 [30]. The most intense growth of micromycetes of the Mucor and Aspergillus genera is found on media containing sucrose, dextrose, maltose, glucose, dextrin, glycerol or plant components (the Sabouraud agar, the Czapek agar, the wort agar, the Grigoraki medium, and the Plout medium).
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Ulrich, Sebastian, and Cornelius Schäfer. "Toxin Production by Stachybotrys chartarum Genotype S on Different Culture Media." Journal of Fungi 6, no. 3 (September 2, 2020): 159. http://dx.doi.org/10.3390/jof6030159.

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Stachybotrys (S.) chartarum had been linked to severe health problems in humans and animals, which occur after exposure to the toxic secondary metabolites of this mold. S. chartarum had been isolated from different environmental sources, ranging from culinary herbs and improperly stored fodder to damp building materials. To access the pathogenic potential of isolates, it is essential to analyze them under defined conditions that allow for the production of their toxic metabolites. All Stachybotrys species are assumed to produce the immunosuppressive phenylspirodrimanes, but the highly cytotoxic macrocyclic trichothecenes are exclusively generated by the genotype S of S. chartarum. In this study, we have analyzed four genotype S strains initially isolated from three different habitats. We grew them on five commonly used media (malt-extract-agar, glucose-yeast-peptone-agar, potato-dextrose-agar, cellulose-agar, Sabouraud-dextrose-agar) to identify conditions that promote mycotoxin production. Using LC-MS/MS, we have quantified stachybotrylactam and all S-type specific macrocyclic trichothecenes (satratoxin G, H, F, roridin E, L-2, verrucarin J). All five media supported a comparable fungal growth and sporulation at 25 °C in the dark. The highest concentrations of macrocyclic trichothecenes were detected on potato-dextrose-agar or cellulose-agar. Malt-extract-agar let to an intermediate and glucose-yeast-peptone-agar and Sabouraud-dextrose-agar to a poor mycotoxin production. These data demonstrate that the mycotoxin production clearly depends on the composition of the respective medium. Our findings provide a starting point for further studies in order to identify individual components that either support or repress the production of mycotoxins in S. chartarum.
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Scognamiglio, T., R. Zinchuk, P. Gumpeni, and D. H. Larone. "Comparison of Inhibitory Mold Agar to Sabouraud Dextrose Agar as a Primary Medium for Isolation of Fungi." Journal of Clinical Microbiology 48, no. 5 (March 10, 2010): 1924–25. http://dx.doi.org/10.1128/jcm.01814-09.

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Csöndes, I. "Effect of culture media on the growth and morphology of Hungarian Macrophomina phaseolina isolates." Acta Agronomica Hungarica 60, no. 2 (June 1, 2012): 109–29. http://dx.doi.org/10.1556/aagr.60.2012.2.3.

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Macrophomina phaseolina (Tassi) Goidanich can cause serious yield losses in several plant species. Investigations were made on the colony morphology of fifty Macrophomina phaseolina isolates from Hungary, one from Spain and two from Serbia on six different culture media to detect differences between the isolates. The fungus exhibited the most intensive growth on maize-flour agar, followed by the Sabouraud-glucose, maltextract, potato-dextrose, Czapek-Dox and watery agar media. Investigations on the macromorphology of the isolates revealed different extents of aerial mycelia formation and differences in the zonal structure of microsclerotial formation on the individual media. One isolate (Mp 38) turned the Sabouraud-glucose agar medium pale pink. Investigations on the micromorphology of the cultures showed that the isolates also varied with respect to microsclerotial characters (size, shape and production of microsclerotia). The microsclerotia of this fungal pathogen had the greatest diameter (averaging 135.31 μm) on Sabouraud-glucose agar medium. This study was the first in Hungary to prove that Macrophomina phaseolina could be grown on nutrient-deficient watery agar, suggesting that it can tolerate a wide range of ecological circumstances.
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Dubljanin, Eleonora, A. M. Dzamic, Sanja Mitrovic, Valentina Arsic-Arsenijevic, and Ivana Colovic-Calovski. "Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods." Archives of Biological Sciences 66, no. 2 (2014): 587–94. http://dx.doi.org/10.2298/abs1402587d.

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The aim of this study was to identify the etiological agents in patients with suspected onychomycosis, and to carry out comparative testing of individual or combinations of tests: direct microscopy with KOH and Blankophor (BP), culturing on Sabouraud?s dextrose agar (SDA), diluted Sabouraud?s dextrose agar (D-SDA) and dermatophyte test medium (DTM). From 70 nail samples (65 toenails, 5 fingernails), 46 (60.5%) had at least one of five positive tests. Isolation was possible in 41, while in 5 samples the presence of fungi was observed by KOH and/or BP. Dermatophytes were most frequently isolated (80.5%) where Trichophyton rubrum was predominant. Candida spp. was isolated in 9.8%, Aspergillus spp. 4.9%, Alternaria spp. 2.4% and Fusarium spp. 2.4%. Application of BP as an individual test was the most sensitive method. The combination of BP with DTM or D-SDA provides the best sensitivity and allows the identification of fungi to the species/genus level.
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Dissertations / Theses on the topic "Sabouraud Dextrose Agar"

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Goupil, Kassandre. "Effects of Liming on Soil Respiration, Fungi Diversity and Abundance in a Metal-Contaminated Region in Northern Ontario." Thesis, Laurentian University of Sudbury, 2014. https://zone.biblio.laurentian.ca/dspace/handle/10219/2196.

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At present, little is known concerning the fungi communities inhabiting the Greater Sudbury Region. This study aimed at identifying the fungal species and abundance in limed and unlimed areas contaminated with metals. Samples were collected from the LFH soil layer from Wahnapitae Hydro-Dam, Daisy Lake, Kingsway, Kelly Lake, Hagar, Onaping Falls and Capreol. Limed and unlimed areas were compared for soil metals, pH, fungi diversity, abundance and seasonal soil respiration. Fungi from soil samples were cultured using Sabouraud Dextrose Agar and Malt Extract Agar. A total of 52 fungi species from 34 genera were identified. There was a significantly higher fungal diversity in the limed areas compared to the samples from unlimed sites based on SDA medium data. Fungi abundance followed the same trend. Significantly higher soil respiration rates were recorded for limed sites compared to unlimed sites. Summer soil respiration rates correlated (r = 0.50) with total fungal abundance.
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Conference papers on the topic "Sabouraud Dextrose Agar"

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Erza, Nurul Nadifa, Fajriati Zulfa, and Yuni Setyaningsih. "Antifungal Test of the Ethanol Extract of Brotowali Stem (Tinospora Crispa) on the Growth of Trichophyton Rubrum in Vitro." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.02.

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ABSTRACT Background: Trichophyton rubrum is an infectious dermatophyte fungus which is the most common cause of dermatophytosis. Fungal resistance and the side effects of therapy are problems of antifungal agents. Phytochemical test of brotowali stem extract (Tinospora crispa) consist of flavonoids, phenols and triterpenoids which have antifungal effects. This study aimed to examine the effectiveness of brotowali stem extract (Tinospora crispa) as antifungal agent towards the growth of Trichophyton rubrum in vitro using agar well diffusion method. Subjects and Method: This was an experimental study using brotowali stem extract with concentration of 10%, 12,5%, 25%, 30%, 40%, 50%, 60%, 75%, and 100%. The dependent variable was Trichophyton rubrum growth. The independent variable was brotowali stem extract (Tinospora crispa). The data were obtained from the inhibition zone showed in agar well diffusion method in Sabouraud Dextrose Agar media. The data were analyzed using Kruskal-Wallis test. Results: The average diameter of inhibition zone of each variance (10%, 12,5%, 25%, 30%, 40%, 50%, 60%, 75%, and 100%) were 2.167 mm, 6.367 mm, 7.0 mm, 10.67 mm, 119 mm, 13.07 mm, 15.8 mm, 17.96 mm dan 17.13 mm, respectively, and they were statistically significant (p= 0.001). Conclusion: Brotowali stem extract has weak antifungal effectiveness at concentration 10%, 12,5%, 25%, intermediate antifungal effectiveness at concentration 30% and strong antifungal effectiveness at concentration 40%, 50%, 60%, 75% and 100%. Keywords: antifungal, brotowali stem, well diffusion, Trichophyton rubrum Correspondence: Fajriati Zulfa. Faculty of Medicine, Universitas Pembangunan Nasional ‘Veteran’ Jakarta. Jl. RS Fatmawati, Pondok Labu, Jakarta Selatan 12450, Telp. (021) 7656971. Email: nurulnerza@gmail.com DOI: https://doi.org/10.26911/the7thicph.05.02
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Montana, Fajar Daniswara, Yuni Setyaningsih, and Fajriati Zulfa. "Effectiveness of Cocoa (Theobroma Cacao L.) Seed Extract on the Growth of in Vitro Malassezia Furfur." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.01.

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ABSTRACT Background: Pityriasis versicolor or Tinea versicolor is a skin disease caused by the Malassezia furfur which is often found in Indonesia. People can use anti-fungal drugs to treat this disease. However, long-term use of anti-fungal drugs is relatively more expensive and can have side effects for its users. Cocoa bean husk contains flavonoids, saponins, and alkaloids which have anti-fungal effects. This study aimed to determine the antifungal effectiveness of the cocoa bean husk extract on the growth of M. furfur. Subjects and Methods: This was an experimental study using cocoa bean husk extract with a concentration variance of 25%, 50%, 75%, 100%, with a positive control for ketoconazole 2% and a negative control using distilled water. The test was carried out by the well diffusion method using Sabouraud Dextrose Agar media. The inhibition of fungal growth was calculated by looking at the clear zone formed after 48 hours. Data were analyzed using Kruskal-Wallis and Post hoc Mann Whitney statistical tests. Results: The mean diameter of the inhibition zone at a concentration of 25%, 50%, 75% and 100% was 3.42 mm, 4.07 mm, 4.9 mm, and 7.3 mm, respectively, and it was statistically significant (p = 0.001). Conclusion: Cocoa bean husk extract has weak anti-fungal effectiveness at concentrations of 25%, 50%, and 75%, while at 100% it has moderate effectiveness. Keywords: antifungal, Pityriasis versicolor, cocoa bean shell, well diffusion, Malassezia furfur Correspondence: Yuni Setyaningsih. Department of Parasitology, Faculty of Medicine, Universitas Pembangunan Nasional “Veteran” Jakarta. DOI: https://doi.org/10.26911/the7thicph.05.01
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