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Journal articles on the topic 'Sabouraud dextrose agar media and cup-plate method'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

K.Ramanji, Reddy* Dr.Chandaka Madhu Dr.J.Balaji Chandra Mouli Dr.B.Jagan Mohan Reddy Penumala Joshi Ballavarapu Sharanya. "ESTIMATION OF PHYTOCHEMICAL SCREENING, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY ON ETHANOL EXTRACT OF SOLANUM LYCOPERSICUM, POUTERIA SAPOTA, MUSA ACUMINATA, AND BETA VULGARIS." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 2808–16. https://doi.org/10.5281/zenodo.888112.

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Even though pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by microorganisms has increased. In general, bacteria have the genetic ability to transmit and acquire resistance to drugs, which are utilized as therapeutic agents . so the present study is to know that the effiency of the herbal extacts on microbical activity(Pouteria Sapota , ,Solanum lycopersicum ,Musa Acuminata, and betavulgaris. Estimation of Antimicrobial activity and antifungal activity on Ecoil,bacillus,salmonella,candida and aspergillus by using Soyabe

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U., Ajima, Ogbonna A.I., Olotu P.N., and Asuke A.U. "EVALUATION OF FUNGAL SPECIES ASSOCIATED WITH DRIED OGI." Continental J. Food Science and Technology 5, no. 1 (2011): 17–25. https://doi.org/10.5281/zenodo.7281485.

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Ogi is a fermented maize product usually used for weaning children in parts of Southern Nigeria. It is sometimes processed by sun drying to preserve and increase its shelf life. This study involves an investigation on the species of fungi associated with dried Ogi.. Soil-Plate isolation method was employed to isolate the fungal species on two conventional fungal culture media (Sabouraud Dextrose Agar and Potato Dextrose Agar). The experimental dried Ogi was assessed for its pH and percentage moisture content. A total of 10 species of fungi belonging to 8 genera were isolated. Three (3) species

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3

Terrones-Fernandez, Ines, Lourdes Rodero-De-Lamo, Asunción López, et al. "Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap." Applied Sciences 14, no. 6 (2024): 2340. http://dx.doi.org/10.3390/app14062340.

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There is currently increased interest in the use of alternatives to autoclaved culture media, in order to maintain the properties of the media, while saving energy and time. In this study, we assess a new system for culture media preparation, using a conventional microwave with a water bath and a glass bottle with a rubber cap that allows depressurization. Sterilization, using the proposed system (1000 W, 3 to 20 min), was compared with autoclaving for the preparation of tryptone soy agar (TSA), tryptone soy broth (TSB), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG)

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4

Rizqi Aminnullah. "Effectiveness of Actinomycetes Isolates from Bogor Botanical Gardens Land as Antifungal against Candida albicans Growth in Vitro." BIOEDUSCIENCE: Jurnal Pendidikan Biologi dan Sains 4, no. 1 (2020): 90–96. http://dx.doi.org/10.29405/j.bes/4190-964362.

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Background: Actinomycetes are Gram-positive and anaerobic bacteria noted for its rod-shaped forms. Actinomycetes aso has an antibiotic and antifungal activity. This study aims to identify the effectiveness of Actinomycetes isolate as the antifungal toward C. albicans growth by in vitro. Methods: Collected samples were planted on Starch Casein Agar (SCA) through pour plate method with three dilution series of 10-4, 10-5,10-6. The media used to see the obstacles zone of C. albicans was Sabouraud Dextrose Agar (SDA) with well method diffusion. Results: Actinomycetes isolates found in the samples

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5

International, Journal of Medical Science and Innovative Research (IJMSIR). "Speciation and anti-fungal susceptibility testing of candida isolates from various clinical samples in a tertiary care hospital, Hyderabad." International Journal of Medical Science and Innovative Research (IJMSIR) 9, no. 3 (2024): 37–42. https://doi.org/10.5281/zenodo.15406789.

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<strong>Abstract</strong> The objective of present study is to speciate the Candida albicans and nonalbicans by using selective, differential media and methods and to determine the anti-fungal susceptibility pattern of the isolates. Biological samples of urine, surgical biopsy (FESS), blood were processed by routine culture methods. All the Candida isolates were speciated by using differential methods like germ tube test, sugar fermentation test, inoculation on media such as Sabouraud dextrose agar, MALT extract agar, Cornmeal -Tween 80 agar and CHROMagar and anti-fungal susceptibility was per

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Shrestha, Bijay Kumar, and Jenish Shakya. "In-Vitro Evaluation of Biofilm and Hemolysis activity of Candida albicans Isolated from Oral Cavity." International Journal of Applied Sciences and Biotechnology 8, no. 4 (2020): 394–99. http://dx.doi.org/10.3126/ijasbt.v8i4.32971.

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Candida albicans is a member of the healthy human microflora, colonizing several niches in the body and can cause opportunistic infection under host debilitated and immunocompromised condition. The present study aimed to investigate the in-vitro hemolytic activity of C. albicans isolated from oral cavity and screen biofilm through three different methods. During the study, 200 oral rinse samples from general human population were analyzed in microbiology laboratory of Central Campus of Technology, Tribhuvan University, Hattisar, Dharan. Nepal. Candida albicans were isolated and identified by c

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Jumare, F. I., M. I. Ahmad, N. Abdulkadir, A. Y. Bello, M. Gani, and M. H. Usman. "Characterization of microorganisms associated with the degradation of sawdust and woodchips." Bayero Journal of Pure and Applied Sciences 15, no. 1 (2022): 69–73. http://dx.doi.org/10.4314/bajopas.v15i1.9.

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Microorganisms play a vital role in the degradation of organic matters such as Sawdust and woodchips. In this study, the Serial dilution method and pour plate techniques were used according to microbiological standards. The media used were nutrient agar, sabouraud dextrose agar, and cellulolytic medium to identify microorganisms and inoculated them into the Sawdust and woodchips and kept for 30 days at 37oC and 25oC. The total viable bacterial count for Sawdust and woodchips ranged between 9.0×103 - 6.0×103and 1.96×103 - 1.48×103, respectively. A total of 12 organisms were identified according

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8

Astuti, Pudji, Achmad Gunadi, and Andania Ulfa Yuga Prasetyaningrum. "Effectiveness biduri leaf extract (Calotropis gigantea) as a denture cleanser in acrylic immersion against the growth of Candida albicans: an experimental laboratory." Padjadjaran Journal of Dentistry 36, no. 1 (2024): 1. http://dx.doi.org/10.24198/pjd.vol36no1.52834.

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ABSTRACTIntroduction: The high number of cases of tooth loss in Indonesia has led to the emergence of dentures. Dentures not appropriately kept clean can trigger cases of denture stomatitis due to Candida albicans. C. albicans is often detected on denture plates and causes denture stomatitis. Cleaning the dentures can prevent denture stomatitis, and chemical cleaning often uses synthetic materials such as alkaline peroxide. However, alkaline peroxide can cause side effects in the form of increased porosity on the surface of the acrylic plate, so an alternative natural ingredient, namely biduri

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9

Nurfadillah, Nurfadillah, Hartati Hartati, and Sulfiani Sulfiani. "Identifikasi Jamur Dermatofita Penyebab Tinea unguium Pada Kuku kaki Petani di Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai." Kampurui Jurnal Kesehatan Masyarakat (The Journal of Public Health) 3, no. 2 (2021): 84–92. http://dx.doi.org/10.55340/kjkm.v3i2.498.

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Tinea unguium is an infection of the nail plate caused by the fungus Dermatophyta. The majority of the livelihood in Dusun Ballakale Desa Aska Kecamatan Sinjai selatan Kabupaten Sinjai is farming where the habit of farmers in this area is to work on rice fields that are in direct contact with soil, water and mud for a long time without wearing protective equipment, one of which is boots to protect the feet. Nails that are in direct contact with soil or mud for a long time and are rarely cleaned can trigger fungal growth. The purpose of this study was to determine the presence or absence of a d

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Abidullah, Mohammed, Arshiya Sanober, Sandeep Kumar, et al. "Salivary Candida albicans in asthmatic patients taking anti-asthmatic medication." Journal of Medicine and Life 15, no. 9 (2022): 1110–14. http://dx.doi.org/10.25122/jml-2022-0142.

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Anti-asthmatic medication makes the oral habitat susceptible to opportunistic infections like Candida, causing oral candidiasis. This study aimed to estimate salivary Candida Albicans in asthmatic patients taking anti-asthmatics medication. A prospective study was performed at the Oral Pathology and Microbiology Department of S.B. Patil Dental College and Hospital, Bidar, Karnataka, India, between June 2018 to November 2018. The research comprised a total of 100 individuals, 50 of whom were asthmatics, and 50 healthy controls who were age and sex-matched to the asthmatics. Saliva was collected

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Pandya, Vanshree, and Sudhir Kumar Jain. "ISOLATION AND MORPHOLOGICAL IDENTIFICATION OF FUNGI FROM DETERIORATING MONUMENTS OF MADHYA PRADESH, INDIA." Journal of Advanced Scientific Research 13, no. 07 (2022): 72–78. http://dx.doi.org/10.55218/jasr.202213708.

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Fungi are the most commonly occurring microbes found on the surfaces of stone. They have very simple nutritionalrequirements and can absorb organic compounds from the environment. Fungal hyphae penetrate surfaces and growinside the soil. This investigation was carried out in order to determine the diverse community of fungi growing on thesedeteriorating monuments. For this study, 55 samples of stone were collected from 10 ancient sites which weredeteriorating. The samples were collected by use of scalpels, brushes, tweezers and stored in UV sterilised poly bags.The isolation was done by using

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12

Wijdan Ahmed Ali, Bahrouz M.M AL-Jaaf, and SajidS.Saleam AL-SaeedI. "Detection of Unculturable Fungi in Soil Regions of Aziz Awa in Sulaimani province." Tikrit Journal of Pure Science 23, no. 9 (2023): 32–38. http://dx.doi.org/10.25130/tjps.v23i9.810.

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Unculturable and non-sporulting fungi represent a great challenge instudying biotrophic, endotrophic and mycorrhizal fungal groups. In thisstudy collection of soil sample from region Aziz Awa in sulaimaniprovince and using culture-dependent and culture in-dependenttechniques for cultivation of unculturable fungi for the purpose ofexpanding studies on fungal biodiversity in soil. Sabouraud DextroseAgar with supplement as pyridoxine by replicating master plate fromhigher dilution of soil three tinny colonies (less than 1mm in diameter)had grown on medium after incubation for 5-7 weeks at 280C bu

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13

Duarte Dias, Ludmila, Guilherme Rodriguez Amando, Francisco Montagner, and Tiago André Fontoura de Melo. "Microbiological analysis of different prefabricated fiberglass posts." Journal of Research in Dentistry 12, no. 3 (2025): 22–27. https://doi.org/10.59306/jrd.v12e3202422-27.

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Objective: This study aims to evaluate the presence of microorganisms in fiberglass posts available in the dental market, aiming to better understand the contribution of these devices to maintaining asepsis after their insertion into the root canal. Materials and methods: Fifty-four smooth conical fiberglass posts from six different commercial brands were selected (n=9 for each brand): Whitepost DC-E®, Exacto®, Fiber Post®, Superpost®, Power Point®, and Rebilda Post®. Microbiological analyses were conducted by submerging the posts in tubes containing Brain-Heart Infusion (BHI) broth and then i

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14

Hardani, Ririen, I. Kadek Adi Krisna, Baharuddin Hamzah, and Muhammad Fakhrul Hardani. "Uji Anti Jamur Ekstrak Buah Mengkudu (Morinda citrifolia L.)." Jurnal IPA & Pembelajaran IPA 4, no. 1 (2020): 92–102. http://dx.doi.org/10.24815/jipi.v4i1.16579.

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Noni is a plant that has the potential to be used as traditional medicinal ingredients. This study aims to determine the inhibition of noni extracts (Morinda citrifolia L.) in inhibiting the growth of the fungus Candida albicans. This type of research is experimental. A total of 150 grams of dried noni fruit that has been mashed, macerated with three different types of solvents namely aquades, acetone and n-hexane to obtain thick extract. Preparation of the fungus suspension was Candida albicans made by mixing the media Nutrient Broth with 5 ose test fungus that were equated with turbidity of

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15

Erina, Erina, Roslizawaty Roslizawaty, and Sri Wahyuli. "Isolasi Candida sp. dan Aspergilus sp. pada Tembolok (Ingluviens) Ayam Ras dan Ayam Buras di Pasar Peunayong, Banda Aceh." Jurnal Agripet 19, no. 1 (2019): 51–58. http://dx.doi.org/10.17969/agripet.v19i1.13162.

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ABSTRAK. Penelitian ini bertujuan mengisolasi Candida sp. dan Aspergillus sp. pada tembolok ayam ras dan buras. Sampel dalam penelitian ini adalah tembolok ayam ras dan buras masing-masing berjumlah 15 sampel yang diambil secara acak dari tempat pemotongan unggas Peunayong Banda Aceh. Isolasi Candida sp. dan Aspergillus sp. dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang diberi antibiotik selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pengamatan morfologi Candida sp. dan Aspergil

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16

Phee, Lynette, Lee Ling Kwang, and Tong Yong Ng. "715. Evaluation of Four Chromogenic Selective Media for the Isolation of Candida auris." Open Forum Infectious Diseases 8, Supplement_1 (2021): S457. http://dx.doi.org/10.1093/ofid/ofab466.912.

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Abstract Background Candida auris is an emerging healthcare-associated multi-drug resistant pathogen able to cause severe invasive infections. Public health agencies have emphasized the need for active surveillance of C. auris to enable prompt infection control measures in healthcare institutions. Four commercially available chromogenic selective media were evaluated for the isolation of C. auris in this study. Methods Four chromogenic selective media (Brilliance Candida (BC), CHROMagar Candida (CC), CHROMagar Candida Plus (CCP) and chromID Candida (CAN2)) were evaluated with 52 fungal and 20

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17

Fatmasari, Vivi Indah, Rebriarina Hapsari, Nadia Hardini, and Endang Sri Lestari. "THE EFFECT OF VCO TO THE GROWTH OF CANDIDA ALBICANS ON DENTURE BASIS OF ACRYLIC RESIN." DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) 9, no. 6 (2020): 442–47. http://dx.doi.org/10.14710/dmj.v9i6.29329.

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Background: Acrylic resin was a material of denture base which frequently used, but it still had a weakness that it was porous, then, it was often the breeding ground of Candida albicans. Virgin coconut oil (VCO) contained fatty acid which was effective to hamper the growth of C.albicans. Objective: This research was to identify the effect of VCO on the growth of C.albicans on acrylic resin denture. Methods: This research was an experimental laboratory research which exerted post-test control group design. The total sample 24 which were divided into 3 groups of treatment and 1 group of control

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18

Blaser, Frank, Isabelle Meneau, Jana Schneider, et al. "Comparison of Analytical Methods for the Validation of the Sterility of Autologous Serum Eye Drops." Klinische Monatsblätter für Augenheilkunde, February 27, 2025. https://doi.org/10.1055/a-2468-5416.

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Abstract Purpose For specific samples, there are different methods and requirements for testing sterility. Blood and blood products are analyzed using automated blood culture methods, while pharmacopeial sterility is assessed using membrane filtration or direct inoculation according to the European Pharmacopoeia (Ph. Eur. 2.6.1.) to detect microbial growth. As autologous serum eye drops (ASEDs) may be viewed as both a blood product and a classical drug, the Swiss legislator has classified these as non-standardizable drugs, and thus, the pharmacopeial requirements apply. This study investigates

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19

Taylor, Denise, Brittney Lynn, Kimberly Trosch, Daniel Marsh, Laurie Grier, and Alexandre Malek. "P-356. Reduction of high-touch surface aerobic bacteria and fungi colony counts with the implementation of ActivePure continuous disinfection technology." Open Forum Infectious Diseases 12, Supplement_1 (2025). https://doi.org/10.1093/ofid/ofae631.557.

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Abstract Background As hospitals nationwide rebound from the effects of the COVID pandemic, reduced staffing and increased hospital-acquired infections (HAIs) are still challenges. Surface disinfection plays a pivotal role in the prevention of HAIs. To improve high-touch surface disinfection and remove the dependence on the human factor, ActivePure continuous disinfection technology devices were installed in the Medical Intensive Care Unit (MICU) HVAC system at Ochsner LSU Health Shreveport in October 2023. ActivePure is a novel technique that kills microbes in the environment by using an adva

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