Relevant bibliographies by topics / Saccharase

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Saccharase'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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Journal articles on the topic "Saccharase"

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Kátai, János, Zsolt Sándor, Magdolna Tállai, and Ágnes Zsuposné Oáh. "Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles." Acta Agraria Debreceniensis, no. 70 (October 24, 2016): 33–39. http://dx.doi.org/10.34101/actaagrar/70/1814.

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Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.
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J, Bayarmaa, and Purev D. "Enzyme activity of zhargalant farm soil, central province of Mongolia." Mongolian Journal of Agricultural Sciences 22, no. 03 (May 9, 2018): 109–13. http://dx.doi.org/10.5564/mjas.v22i03.953.

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We conducted monitoring analysis of cellulase, saccharase, protease, urease, acid and alcaline phosphatase activities of Zhargalant farm soil, Central province of Mongolia. From the results it is clear seen that for the year activity of cellulase, protease and urease are increased but activity of saccharase decreased. The activity of acid phosphatase on control, wheat and nearby wheat field soils decreased but on rape and nearby rape fields its activity increased. About alkaline phosphatase its activity decreased on control and soil of wheat field, on soils of nearby wheat, rape and nearby rape fields its activity increased. For the field where seeding crops did not produces there was positive correlation between humus and enzyme activity, but for the soils were the crops were sown this correlation changes depending on the enzymes. This trend is also observed for the soils of nearby fields.
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Jensen, Poul Erik. "Familial Saccharase Deficiency Entailing Intolerance to Cane Sugar." Acta Paediatrica 52 (January 21, 2008): 119. http://dx.doi.org/10.1111/j.1651-2227.1963.tb08743.x.

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Stano, Ján, Peter Siekel, Karol Mičieta, and Alfred Barth. "Study of immobilized and extracellular saccharase of watermelon." Acta Histochemica 108, no. 5 (November 2006): 401–6. http://dx.doi.org/10.1016/j.acthis.2006.05.003.

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Brezovcsikné, Maria Antal, and Attila Anton. "Comparative Studies on Saccharase Activity of Different Hungarian Soils." Zentralblatt für Mikrobiologie 141, no. 7 (1986): 495–501. http://dx.doi.org/10.1016/s0232-4393(86)80001-4.

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Zhao, Fazhu, Jieying Wang, Lu Zhang, Chengjie Ren, Xinhui Han, Gaihe Yang, Russell Doughty, and Jian Deng. "Understory Plants Regulate Soil Respiration through Changes in Soil Enzyme Activity and Microbial C, N, and P Stoichiometry Following Afforestation." Forests 9, no. 7 (July 20, 2018): 436. http://dx.doi.org/10.3390/f9070436.

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Soil respiration (SR) is an important process in the carbon cycle. However, the means by which changes in understory plant community traits affect this ecosystem process is still poorly understood. In this study, plant species surveys were conducted and soil samples were collected from forests dominated by black locust (Robinia pseudoacacia L.), with a chronosequence of 15, 25, and 40 years (RP15, RP25, and RP40, respectively), and farmland (FL). Understory plant coverage, evenness, diversity, and richness were determined. We investigated soil microbial biomass carbon (MBC), nitrogen (MBN), phosphorus (MBP), and stoichiometry (MBC:MBN, MBC:MBP, and MBN:MBP). Soil enzyme assays (catalase, saccharase, urease, and alkaline phosphatase), heterotrophic respiration (HR), and autotrophic respiration (AR) were measured. The results showed that plant coverage, plant richness index (R), evenness, and Shannon-Wiener diversity were higher in RP25 and RP40 than in RP15. SR, HR, and AR were significantly higher in the forested sites than in farmland, especially for SR, which was on average 360.7%, 249.6%, and 248.2% higher in RP40, RP25, and RP15, respectively. Meanwhile, catalase, saccharase, urease, and alkaline phosphatase activities and soil microbial C, N, P, and its stoichiometry were also higher after afforestation. Moreover, significant Pearson linear correlations between understory plants (coverage, evenness, diversity, and richness) and SR, HR, and AR were observed, with the strongest correlation observed between plant coverage and SR. This correlation largely depended on soil enzymes (i.e., catalase, saccharase, urease, and alkaline phosphatase), and soil microbial biomass C, N, and P contents and its stoichiometry, particularly urease activity and the MBC:MBP ratio. Therefore, we conclude that plant communities are drivers of soil respiration, and that changes in soil respiration are associated with shifts in soil enzyme activities and nutrient stoichiometry.
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Juknevičienė, Edita, Honorata Danilčenko, Elvyra Jarienė, and Jürgen Fritz. "The effect of horn-manure preparation on enzymes activity and nutrient contents in soil as well as great pumpkin yield." Open Agriculture 4, no. 1 (August 21, 2019): 452–59. http://dx.doi.org/10.1515/opag-2019-0044.

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AbstractThis investigation was inspired by an increasing global issue on how to improve soil quality while using alternative preparations instead of synthetic fertilizers. The main aim of a three-year study was to investigate the influence of horn-manure preparation on enzyme activity and nutrient content in soil and pumpkin yield. The results showed that significantly higher amounts of P (respectively 106 and 79 mg kg−1 CAL), K (149 and 106 mg kg−1 CAL), nitrogen (5.41 and 3.21 mg kg−1), ammoniacal nitrogen (9.38 and 3.45 mg kg−1) and mineral nitrogen (7.97 and 5.67 mg kg−1) were measured in the plots where the horn-manure preparation was used. A higher activity of the soil enzymes (urease activity was 1.93 times higher and the saccharase activity was 1.05 times higher) were identified with horn-manure. The average soil CO2 flux (Fc) value, when using horn-manure preparation (from 56 till 70 day), was significantly higher by 5.32% in the middle of the growing season. The yield of pumpkin was significantly increased by 18% with horn manure treatments. Significant positive correlations were identified between pumpkin yield and urease activity, and saccharase activity, as well as soil P and K.
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Malá, Š., P. Karasová, M. Marková, and B. Králová. "Oligosaccharide synthesis using a-glucosidases of different origin." Czech Journal of Food Sciences 19, No. 2 (February 7, 2013): 57–61. http://dx.doi.org/10.17221/6576-cjfs.

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a-Glucosidase from Aspergillus awamori and intestinal a-glucosidase (saccharase-isomaltase complex) exhibited high transglycosylation activity and were able to synthesize tri- and tetrasaccharides during maltose hydrolysis. Both tested enzymes were also able to transfer the glucose residue to all tested monosaccharide acceptors (D-mannose, D-xylose, L-sorbose and D-galactose). Their transfer activity towards respective acceptors varied and their acceptor preference also depended on the origin of the enzyme. Out of the acceptors tested, both enzymes exhibited high transfer activity in xylose.
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Kátai, János, Thomas Döring, Magdolna Tállai, Andrea Balla-Kovács, István Henzsel, Marianna Makádi, Zsolt Sándor, and Imre Vágó. "Influence of alternative plant nutrition methods on soil microbial characteristics in long-term experiments." Agrokémia és Talajtan 67, no. 1 (June 2018): 79–90. http://dx.doi.org/10.1556/0088.2018.67.1.6.

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The size of the arable land is constantly decreasing all over the world due to severe anthropogenic disorders. Plant production therefore has to be adapted to changing environmental conditions along with the proper selection of crop varieties and the application of sustainable environmental technologies which also consider economic aspects. The investigations were carried out in the Westsik long-term fertilization experiment near Nyíregyháza, East Hungary, which was set up in 1929 (89 years ago). Alternative forms of nutrient supplies (A) (green manure, straw with and without fermentation, organic fertilizer with and without inorganic fertilizer supplements) were used in different crop rotations. The test plant was potato (Solanum tuberosum L.) and the soil type sand with a low humus content (Arenosols). A further long-term experiment is located on calcareous chernozem soil (Chernozems) in Debrecen (set up in 1983, 35 years ago). In one part of this experiment, organic farming (OF) has been carried out with a pea, winter wheat and maize crop rotation for over 15 years with no inorganic fertilization. In another block in this experiment, changes in soil properties as a result of the medium and high doses of fertilizers applied in intensive farming (I) were evaluated with a maize (Zea mays L.) monoculture as the test plant. The results obtained with alternative nutrient supplies (green manure, fermented and unfermented straw, farmyard manure, fertilization) proved that the soil organic carbon content increased to varying degrees in humus-poor, acidic sand soil. The organic matter content of the soils increased in response to the treatments, contributing to a significant enhancement in soil microbial parameters (MBC, saccharase, dehydrogenase and phosphatase enzyme activities). The carbon dioxide production and saccharase enzyme activity in organic plots (OF) were significantly lower than in intensively farmed (I) soils. At the same time, in the case of organic farming (OF) the microbial biomass carbon, phosphatase and dehydrogenase activity were significantly higher in OF plots than in I plots. Compared to the control soil, MBC was 7-8 times higher in organic plots and 1.3-3.8 times higher in intensive plots. Organic farming on chernozem soil generally resulted in higher microbial activity (MBC, phosphatase, saccharase and dehydrogenase enzyme activity) than in either intensively farmed chernozem or in the case of alternative farming (A) on sandy soil.
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Feng, Da Lan, Yan Jin, Yu Hong Yang, and Jian Guo Huang. "Distribution and Enzyme Activities in the Soil around the Fertilizes." Advanced Materials Research 610-613 (December 2012): 3027–33. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.3027.

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An incubation experiment was carried out to study various available N pools and enzyme activities in the soil near fertilizers under controlled temperature and soil moisture. Fertilizers added into soil were chemical fertilizer supplied as urea, organic fertilizer as rapeseed straw, and mixture of urea and rapeseed straw in a ratio of 7:3, respectively. 30 days after incubation, NH+4-N, NO-3-N and 1 N NaOH- hydrolyzed N increased in the soil at < 2.5 cm from the fertilizers in two lateral directions, and progressively decreased as the distance to the fertilizers increased. The results indicated the intensive available N release from the fertilizers and easy movement of fertilizer N. Taking into account of dense roots in cultivated soil layers and easy migration of N fertilizers, broadcast application of N fertilizers could be efficient in the middle growing periods of crops. There was neither obvious influence of urea application on urease activity nor significant correlation between urease activity and NH4+-N in the soil. Therefore, it seems reasonable to suggest that urea hydrolysis catalyzed by urease might be fast, unlikely the rate-limiting step in the process of urea transformation into NH4+-N. Further study showed the high activities of saccharase and protease in the soil only at 0.25 cm from the organic fertilizers added either in pure rape straw or mixture with urea. Saccharase and protease on the interface between organic fertilizer and soil could thus accelerate N release of organic fertilizers as available forms through organic N decomposition, resulting in the high available N pools in the soil near organic fertilizers.
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Dissertations / Theses on the topic "Saccharase"

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Joucla, Gilles. "Caractérisation de l'alternane-saccharase de Leuconostoc mesenteroides NRRL B-1355 : approche rationnelle et aléatoire pour la conception de nouvelles glucane-saccharases." Toulouse, INSA, 2003. http://www.theses.fr/2003ISAT0032.

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L'alternane-saccharase (ASR) catalyse, à partir de saccharose, la formation d'un polymère de glucose associé par des liaisons osidiques a-1,6 et a-1,3 alternées. L'objectif de l'étude était de caractériser l'ASR, dont la spécificité est unique dans la famille 70 des glycoside-hydrolases, pour avancer dans la compréhension du mode de synthèse du polymère et de la formation des liaisons alternées. Dans un premier temps, l'expression hétérologue du gène asr dans E. Coli a montré que l'enzyme était faiblement produite et fortement dégradée. La forme ASR C-del (161 kDa), tronquée des séquences répétées de type " APY " et obtenue par délétion du gène asr, a permis de multiplier par un facteur 120 le niveau de production de l'enzyme. Cette forme est très peu dégradée ce qui rend possible la caractérisation de l'activité. L'étude montre que le polymère est synthétisé à partir de l'extrémité non réductrice par transfert successif sur du glucose. S'opposant au mécanisme couramment admis pour les glucane-saccharases, il devra être étayé par des données structurales. Un procédé permettant de produire efficacement l'enzyme et de la purifier a été établi pour pouvoir initier les expériences de cristallisation. D'après des travaux de mutagénèse, le fragment 768YDA770 s'est révélé impliqué dans la spécificité d'alternance. Cependant l'absence de données structurales limite l'identification de nouvelles cibles. C'est pourquoi, nous avons créé des banques de variants par mutagénèse aléatoire et recombinaison, et développé des méthodes de criblage pour l'isolement de variants plus actifs et dépourvus d'activité ASR. Les protocoles ont été testés avec succès sur une fraction de la banque
The alternansucrase (ASR) synthesizes from sucrose an homopolymer of glucose with a-1,6 and a-1,3 alternanted glucosidic linkages. The aim of this work was to characterise the ASR to understand the polymer synthesis and the process for alternanting the linkages which is unique in the 70 family of glucoside-hydrolases. Expression of asr gene in E. Coli led to low production and to degraded forms of the enzyme. However, the form ASR C-del (161 kDa), which is genetically truncated from "APY" amino acids repeated sequences, allowed a 120 fold increase in production without any degradation. Characterisation of the enzyme activity showed that the polymer is synthesized from the non-reducing end with successive transfers on glucose, the initial acceptor. Furthermore, sequence analysis followed with mutant constructions showed that the 768YDA770 fragment has a significant role in the alternating synthesis process. All theses results need to be confirmed by structural data. Thus, we developed a purification process to purify the ASR C-del in order to initiate crystallization experiments. To avoid limited design by the site directed mutagenesis without 3D structure, we generated libraries of variants of ASR C-del by random mutagenesis and combinatorial engineering and developed screening assays to isolate mutants more actives or affected in alternating function. Protocols were successfully set up and tested with the recent high throughput platform
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Willemot, René-Marc. "Etude de la dextrane-saccharase de Leuconostoc mesenteroides NRRL B512F." Toulouse, INSA, 1993. http://www.theses.fr/1993ISAT0031.

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Differentes formes de la dextrane-saccharase de leuconostoc mesenteroides nrrl b-512f ont ete purifiees. Pour cela, le dextrane associe a l'enzyme a tout d'abord ete elimine par action d'une preparation hautement pure de dextranase fongique. Plusieurs protocoles de purification de la dextrane-saccharase sont decrits. Une preparation d'activite specifique egale a 161 u/mg a ete obtenue. L'analyse electrophoretique de l'enzyme conduit a des resultats dependant de la methode de purification utilisee: dans tous les cas un poids moleculaire de 130000 est observe, mais des molecules actives de poids moleculaire 65000 sont egalement obtenues lorsqu'on purifie l'enzyme par ultrafiltration. Il pourrait s'agir d'une forme monomerique active, mais le plus souvent presente sous une forme agregee dans les milieux. L'enzyme presente un point isoelectrique egal a 4. L'effet activateur et stabilisateur du dextrane sur l'enzyme a ete etudie, ceux-ci sont etroitement dependants de la forme enzymatique utilisee. Le calcium n'est pas indispensable a l'activite enzymatique, mais l'edta inhibe partiellement et reversiblement cette activite. La specificite des reactions d'accepteur catalysees par la dextrane-saccharase s'avere trop etroite pour pouvoir utiliser cette enzyme en vue de transferer des unites glucose vers d'autres molecules comme des proteines, des peptides, des acides amines ou des polyosides autres que le dextrane
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Daguer, Smith Jean Pierre. "L'opéron lévane saccharase de Bacillus subtilis : régulation transcriptionnelle et post-transcriptionnelle." Paris 7, 2004. http://www.theses.fr/2004PA077046.

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Aymerich, Stéphane. "Etude de la regulation de la synthese de la levane-saccharase de bacillus subtilis : modulation du niveau de la synthese, induction par le saccharose." Paris, Institut national d'agronomie de Paris Grignon, 1987. http://www.theses.fr/1987INAPA019.

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BENYAHIA, BABAALI FADILA. "Etude du mecanisme de secretion de la levane saccharase chez bacillus subtilis." Paris 6, 1989. http://www.theses.fr/1989PA066553.

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La levane saccharase de bacillus subtilis, enzyme secrete pendant la phase exponentielle de croissance, inductible par le saccharase, representant chez un mutant haut producteur 8 a 10% des proteines membranaires, est utilisee comme modele d'etude du mecanisme de secretion proteique. Une forme lourde membranaire (53 kd) possedant une extension n-terminale de 29 acides amines, deja caracterisee dans les minicellules de e. Coli, hote du gene de structure de la ls et une forme legere membranaire (50 kd) de meme masse que la ls exocellulaire ont ete caracterisees comme intermediaires de secretion. Chez b. Subtilis ou les minicellules de e. Coli la maturation de la forme lourde est inhibee en presence d'agents amphiphiles. Purifiees, les deux formes membranaires sont actives qu'en presence d'ion fe#3#+. La forme lourde est convertie in vitro en forme legere par la leader peptidase de e. Coli. Nous proposons un mecanisme de secretion a deux etapes membranaires: la modification covalente de la forme lourde, la secretion de la forme legere resultante, le fer etant necessaire a cette etape. Le remplacement de la glycine, en position 366 (la ls mature est constituee de 444 acides amines) par un aspartate ou une arginine affecte uniquement la seconde etape. Le niveau de secretion du mutant aspartate est augmente d'un facteur 10 en presence de fer. In vitro, le fer augmente d'un facteur trois la vitesse de repliement de la ls sauvage purifiee. L'implication d'ions metalliques souvent requis pour la secretion du proteines de bacillacees pourrait etre un element general du mecanisme de secretion chez ces bacteries gram#+
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ARGUELLO, MORALES MARTHA ALICIA. "L'alternane-saccharase de leuconostoc mesenteroides nrrl b-1355 : structure primaire et synthese d'oligosides." Toulouse, INSA, 2000. http://www.theses.fr/2000ISAT0009.

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L'alternane-saccharase est une glucane-saccharase excretee par l. Mesenteroides nrrl b-1355. En presence de saccharose, l'enzyme catalyse la synthese d'alternane, un polymere compose de residus glucose associes par des liaisons osidiques 1,6 et 1,3 alternees. De plus, lorsqu'un accepteur tel que le maltose est ajoute dans le milieu, l'alternane-saccharase catalyse le transfert du glucose provenant du saccharose sur l'accepteur pour produire des oligosides de faible masse molaire egalement porteurs de liaisons 1,6 et 1,3 alternees. L'etude realisee a porte sur deux aspects. Tout d'abord, le clonage du gene codant pour l'alternane-saccharase a ete entrepris afin de comparer la structure primaire de cette enzyme aux autres glucane-saccharases connues de specificite differente. En parallele, la reaction en presence de differents accepteurs catalysee par l'alternane-saccharase a ete etudiee afin de cerner l'interet de cette enzyme pour la synthese de nouveaux oligosides. Les premiers travaux de clonage nous ont conduits a isoler un gene codant pour une dextrane-saccharase (dsr-c). Le gene codant pour l'alternane-saccharase (asr) a ete isole a l'aide d'une sonde specifique de la proteine. Les deux genes ont ete sequences et les proteines codees ont ete caracterisees. Le gene dsr-c est identique au gene dsr-b de l. Mesenteroides nrrl b-1299 indiquant que les deux genes proviennent du meme ancetre. L'analyse de la sequence proteique codee par le gene asr a permis d'identifier des segments absents dans les sequences des autres glucane-saccharases, ainsi que des segments differents. Ces regions pourraient etre impliquees dans la specificite de l'enzyme. L'activite alternane-saccharase en presence de differents oses accepteurs a ete comparee a celle de la dextrane-saccharase de l. Mesenteroides nrrl b-512f qui ne synthetise que des liaisons osidiques de type 1,6. Il est apparu de nombreuses differences entre l'alternane-saccharase et la dextrane-saccharase vis a vis des molecules testees, certaines etant mieux reconnues par une enzyme que par l'autre. En particulier, le cellobiose qui etait identifie comme un mauvais accepteur pour la dextrane-saccharase est apparu comme etant efficacement glucosyle par l'alternane-saccharase, avec un rendement de 30%. Les produits obtenus ont ete purifies et leurs structures determinees.
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Molina, Manon. "Exploration of the molecular determinants involved in alternansucrase specificity and stability." Thesis, Toulouse, INSA, 2019. http://www.theses.fr/2019ISAT0010.

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L’alternane-saccharase (ASR) de Leuconostoc citreum NRRL B-1355 est une glucane-saccharase appartenant à la famille 70 des glycoside hydrolases (GH70). Cette α-transglucosylase utilise le saccharose, substrat peu coûteux et abondant, pour catalyser la formation d’un polymère d’α-glucane composé de liaisons osidiques α-1,6 et α-1,3 alternées dans la chaîne principale et appelé alternane. Avec une température optimale de 45°C, l’ASR est parmi les glucane-saccharases les plus stables. Afin d’avoir une meilleure compréhension des déterminants de la spécificité de liaison, de la polymérisation et de la plus haute stabilité de l’ASR, nous avons résolu la structure de cette enzyme. Notre étude par mutagénèse dirigée combinée à du docking moléculaire suggère que la spécificité de liaison est contrôlée par le positionnement de l’accepteur dans l’un ou l’autre des sous-sites +2 ou +2’. Des complexes de l’ASR avec différents ligands ont également mis en évidence un site signature de l’enzyme. Ce site est impliqué dans la formation du polymère d’alternane et pourrait servir de pont facilitant l’élongation processive de l’alternane. Enfin, nos travaux préliminaires indiquent que le domaine C pourrait être impliqué dans la stabilité de ces enzymes. Nos résultats ouvrent des nouvelles pistes d’investigation concernant l’étude des relations structure-fonction des glucane-saccharases et la conception de polymères de structure et propriétés physico-chimiques contrôlées
The alternansucrase (ASR) from Leuconostoc citreum NRRL B-1355 is a glucansucrase belonging to the family 70 of glycoside hydrolases (GH70). This α-transglucosylase uses a cheap and abundant molecule, sucrose, to catalyze the formation of a unique α-glucan polymer made of alternating α-1,6 and α-1,3 linkages in the main chain, called alternan. With a 45°C optimum temperature, ASR is among the most stable glucansucrases to date. To get a deeper insight in ASR determinants involved in linkage specificity, polymerization and stability, we have solved the unliganded 3D structure of this enzyme at 2.8 Å. Coupled to mutagenesis and molecular docking, our results suggest the alternance to be governed by the acceptor positioning in either +2 or +2’ subsite, and the key contributions of Trp675 or Asp772 residue, respectively. Complexes of ASR with various sugar ligands were also obtained and highlighted a site never identified in any other GH70 enzymes. This site is uniquely found in alternansucrase and could act as a bridge between the domain V and the active site facilitating alternan processive elongation. Finally, the construction and characterization of chimera enzymes suggested domain C to be involved in enzyme stability. Overall, our results improved our knowledge on the structure-function relationship of ASR and open new paths for the conception of polymers with controlled structures and physicochemical properties
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HADDAOUI, ELARBI. "Secretion de la levane saccharase et de l'alpha-amylase chez bacillus subtilis : caracterisation de l'etape cinetiquement limitante." Paris 11, 1997. http://www.theses.fr/1997PA112014.

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Le travail presente dans ce memoire est consacre a l'etude de la secretion proteique chez bacillus subtilis. La description du mecanisme de secretion proteique, en termes moleculaires, repose au moins en partie sur l'identification des etapes discretes cellulaires de ce processus. Cet objectif peut etre atteint grace a la caracterisation spatiale et temporelle des formes precurseurs de la proteine secretee completee par l'etude in vitro des reactions simulants les modifications que peut subir la proteine au cours de sa secretion. Deux enzymes secretes par ce microorganisme ont ete choisis comme proteines modeles, la levane saccharase et l'alpha-amylase. Un mecanisme de secretion a deux etapes a ete anterieurement propose pour la levane saccharase. J'ai contribue a l'identification des catalyseurs possibles de la deuxieme etape, etape cinetiquement limitante s'effectuant sur la face externe de la membrane et au cours de laquelle l'evenement de liberation de la proteine dans le milieu exterieur est etroitement couple a l'acquisition de la structure native resistante aux proteases. Avec l'alpha-amylase, j'ai entrepris la caracterisation des formes intermediaires cellulaires. Lorsque l'alpha-amylase est exprimee sous le controle de son propre promoteur amyri, aucune forme transitoire ne peut etre detectee. Cette recherche m'a conduit cependant a caracteriser une nouvelle amylase endocellulaire chez b. Subtilis. A un niveau de synthese plus eleve, obtenu en placant le gene amye sous le controle de sacr, j'ai identifie une forme precurseur transitoire, depourvue de peptide signal. Le temps de demi-liberation de cette forme est du meme ordre de grandeur que le temps de demi-reaction du repliement de la proteine in vitro. Les deux evenements exigent la presence de calcium. Ainsi, le processus de secretion de l'alpha-amylase et de la levane saccharase pourrait s'effectuer post-traductionnellement selon une sequence assez semblable de deux etapes discretes. L'efficacite de la seconde etape, cinetiquement limitante, couplee au repliement proteique pourrait dependre de la presence de catalyseurs de repliement sur la face externe de la membrane. La comparaison des proprietes de repliement de ces deux proteines revele des caracteristiques communes qui pourraient aider a definir un code interne de secretion des proteines chez b. Subtilis.
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Dumond, Pascale Morali Alain. "Le déficit congénital en saccharase-isomaltase étude rétrospective de 53 cas diagnostiqués en France de 1963 à 2003 /." [S.l.] : [s.n.], 2006. http://www.scd.uhp-nancy.fr/docnum/SCDMED_T_2006_DUMOND_PASCALE.pdf.

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Dols, Marguerite. "Etude de la dextrane-saccharase de Leuconostoc mesenteroides NRRL B-1299 : production et application à la synthèse d'oligosides." Toulouse, INSA, 1996. http://www.theses.fr/1996ISAT0026.

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La dextrane-saccharase de leuconostoc mesenteroides nrrl b-1299 catalyse la synthese de dextrane, polymere de glucose, a partir du saccharose et, en presence de saccharose et maltose, la synthese d'oligosides contenant des liaisons (12) osidiques, molecules d'interet industriel. Une etude du metabolisme de la bacterie en presence de fructose, glucose et saccharose montre que la production de dextrane-saccharase par l. Mesenteroides est soumise a un systeme complexe de regulation faisant intervenir le saccharose (inducteur) et le fructose (regulation negative). De ce fait, il est difficile d'ameliorer le titre final d'activite dextrane-saccharase obtenu par les techniques couramment employees. D'autres methodes, inspirees par l'etude metabolique, sont donc mises au point: l'utilisation de glucose comme co-substrat du saccharose permet ainsi de doubler la quantite d'enzyme produite. Par ailleurs, l'etude du metabolisme du glucose et du fructose revele la presence d'une nouvelle dextrane-saccharase minoritaire, non induite par le saccharose. Les deux formes enzymatiques de la dextrane saccharase (dss soluble et dsi insoluble) produites sur saccharose sont concentrees et caracterisees. Un plan d'experience permet ensuite de determiner les conditions optimales de synthese d'(12) oligosides avec l'enzyme dsi libre. Les produits sont purifies par chromatographie, puis analyses par spectrometrie de masse et rmn du carbone 13. Un modele cinetique des premieres etapes de la reaction est etabli, qui permet de predire les performances de la reaction de synthese de l'oligoside panose (dp 3), avec l'enzyme utilisee en reacteur a lit fixe. Enfin, l'enzyme est encapsulee dans des billes d'alginate. L'efficacite maximale d'immobilisation est obtenue avec de faibles teneurs en alginate, avec une activite specifique de 4 u/g de support. Mais, la reaction est affectee par des problemes de diffusion interne, qui modifient le comportement enzymatique et rendent la synthese d'(12) oligosides moins efficace qu'avec l'enzyme dsi libre. Ce phenomene est accru lorsque l'enzyme est utilisee en reacteur a lit fixe, par l'apparition de problemes de diffusion externe, quelle que soit la configuration de reacteur envisagee
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Books on the topic "Saccharase"

1

D, Phillips Marcus, and Shinkai Seiji, eds. Boronic acids in saccharide recognition. Cambridge: RSC Publishing, 2006.

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Hartley, James Holroyd. Saccharide accelerated hydrolysis of boronic acid imines. Birmingham: University of Birmingham, 2000.

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Derbyshire, Helen M. Physical properties of hydrated saccharides and saccharide derivatives. Leicester: De Montfort University, 2000.

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Le saccharose. Economica, 1995.

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Conformational Analysis Saccharide. CRC, 1999.

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Boronic Acids in Saccharide Recognition. Cambridge: Royal Society of Chemistry, 2006. http://dx.doi.org/10.1039/9781847557612.

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Samuel, Jessy. Adverse events of intravenous iron dextran and intravenous iron saccharate. 1999.

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James, Tony D., Marcus D. Phillips, Seiji Shinkai, and J. Fraser Stoddart. Boronic Acids in Saccharide Recognition (Monographs in Supramolecular Chemistry). Royal Society of Chemistry, 2006.

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Nelson, Michael J., and Popink. Happy Kitty Bunny Pony: A Saccharine Mouthful of Super Cute. Harry N. Abrams, 2005.

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Popink. Happy Kitty Bunny Pony: A Saccharine Mouthful of Super Cute. Tandem Library, 2005.

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Book chapters on the topic "Saccharase"

1

Stellmach, Bruno. "Saccharase." In Bestimmungsmethoden Enzyme, 258–61. Heidelberg: Steinkopff, 1988. http://dx.doi.org/10.1007/978-3-642-93668-5_31.

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Tauber, R., and F. H. Perschel. "Saccharase-Isomaltase." In Springer Reference Medizin, 2089. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_2728.

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Tauber, R., and F. H. Perschel. "Saccharase-Isomaltase." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_2728-1.

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Schinner, Franz, Richard Öhlinger, and Ellen Kandeler. "Bestimmung der Saccharase-Aktivität." In Bodenbiologische Arbeitsmethoden, 57–60. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-97284-3_14.

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Lück, Erich. "Saccharose." In Chemische Lebensmittelkonservierung, 118–22. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-96924-9_19.

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Bährle-Rapp, Marina. "Saccharose." In Springer Lexikon Kosmetik und Körperpflege, 485. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_9058.

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Lück, Erich, and Martin Jager. "Saccharose." In Chemische Lebensmittelkonservierung, 131–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-57868-7_15.

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Baležentienė, Ligita. "Indicating Soil Quality Using Urease and Saccharase Activity in Abandoned Grassland and Differently Managed Crop Fields." In Quantitative Traits Breeding for Multifunctional Grasslands and Turf, 387–94. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9044-4_53.

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Kohaupt, Burchard. "Kohlenhydrate (Saccharide)." In Praxiswissen Chemie für Techniker und Ingenieure, 115–16. Wiesbaden: Vieweg+Teubner Verlag, 1996. http://dx.doi.org/10.1007/978-3-663-07703-9_15.

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Bährle-Rapp, Marina. "Saccharated Lime." In Springer Lexikon Kosmetik und Körperpflege, 485. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_9036.

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Conference papers on the topic "Saccharase"

1

Krug, W. P., and M. Aronson. "Novel Saccharide Piezoelectric Composites." In Sixth IEEE International Symposium on Applications of Ferroelectrics. IEEE, 1986. http://dx.doi.org/10.1109/isaf.1986.201199.

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Alonso, Elena, José Alonso, and Santiago Mata. "ROTATIONAL SPECTRUM OF SACCHARINE." In 72nd International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2017. http://dx.doi.org/10.15278/isms.2017.te03.

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Schechner, Pinchas, Lea Mor, Shlomo Kimchie, Hussein Tarabeah, Carlos Dosoretz, and Kas Hemmes. "Saccharide Fuel Cell (SFC)." In ASME 2004 2nd International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2004. http://dx.doi.org/10.1115/fuelcell2004-2511.

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A study on the possibility to use saccharides as fuels in a Fuel Cell is presented. The study deals with the abundance of saccharides and ways to extract them from solid organic urban, forest and agricultural wastes, and from food industry effluents. The use of saccharides as fuel is treated from the thermodynamic point of view and compared with other common fuels currently used in fuel cells. Other properties of saccharides, relevant to their use as fuels, such as: safety, transportability, storage, inflammability, poisonous character and volatility, are also considered. The different possible catalytic electrodes needed to create a Saccharide Fuel Cell are discussed. Three options are considered: Microbiological, Enzymatic and Inorganic. None of the available catalytic electrodes has satisfactory performance. We conclude that since sacharides are human friendly, abundant, have high-energy content and are relatively easy to extract, efforts should be given to develop a Saccharide Fuel Cell. These fuel cells have the potential to become the basis of a decentralized power economy and open economical ways to deal with the environmental problems caused by organic wastes. The concept exposed in this paper will be tested in a Pilot-Demonstration Project, planned in the Agan Beit Natufa (ABN) region in Israel. We estimate a production of about 11 GWh/year from this project.
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Glibitskiy, G. M. "Energy of activation of saccharose in solutions." In 2010 International Kharkov Symposium on Physics and Engineering of Microwaves, Millimeter and Submillimeter Waves (MSMW). IEEE, 2010. http://dx.doi.org/10.1109/msmw.2010.5546083.

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Wang, Deyu, Duxiao Jiang, and Chunwei Yuan. "Spectral characters of lectin saccharide interaction." In International Symposium on Biomedical Optics, edited by Qingming Luo, Britton Chance, Lihong V. Wang, and Steven L. Jacques. SPIE, 1999. http://dx.doi.org/10.1117/12.364383.

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Kowalczyk, Wioleta, Julie Sanchez, Philippe Kraaz, Laurence Meagher, David Haylock, Oliver E. Hutt, and Peter J. Duggan. "Peptide-Boronic Acid Libraries for Saccharide Recognition." In The Twenty-Third American and the Sixth International Peptide Symposium. Prompt Scientific Publishing, 2013. http://dx.doi.org/10.17952/23aps.2013.104.

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Kalbarczyk, K. Z., M. A. Koffas, and C. H. Collins. "Development of an assay for saccharide detection." In 2015 41st Annual Northeast Biomedical Engineering Conference (NEBEC). IEEE, 2015. http://dx.doi.org/10.1109/nebec.2015.7117170.

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Bourhill, Grant, Kamjou Mansour, Kelly J. Perry, Lutfur R. Khundkar, Edward T. Sleva, Roger Kern, Joseph W. Perry, Ian D. Williams, and Stewart K. Kurtz. "Second-order nonlinear optical properties of saccharide materials." In OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, edited by Peter M. Rentzepis. SPIE, 1993. http://dx.doi.org/10.1117/12.144052.

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Librizzi, Fabio. "Distribution of A substates in saccharide coated Carbonmonoxy-Myoglobin." In Fifth scientific conference on nuclear and condensed matter physics. AIP, 2000. http://dx.doi.org/10.1063/1.1303345.

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Szczygielska, Aneta, Andrzej Burian, John C. Dore, S. Duber, and A. Hannon. "Paracrystalline nature of saccharose- and anthracene-based carbons studied by wide-angle scattering." In SPIE Proceedings, edited by Jaroslaw Rutkowski and Antoni Rogalski. SPIE, 2003. http://dx.doi.org/10.1117/12.519677.

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