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Dissertations / Theses on the topic 'Saccharomyces cerevisiae Gene Expression Regulation'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Wood, Rachel M. C. "Translational regulation of gene expression in Saccharomyces cerevisiae." Thesis, University of Aberdeen, 1994. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU540996.

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The over-expression of the Saccharomyces cerevisiae pyruvate kinase gene (PYK1) is limited at the level of transcription and translation (Moore et al, 1990a). High levels of PYK1 mRNA are not tolerated by the transformants which grow up to 5.2-fold slower than the untransformed host strain. In addition, the transformants are genetically unstable, reverting at high frequency to fast growing cells via plasmid loss (Moore et al., 1990c). A novel experimental system was designed to facilitate the analysis of the translational regulation of the PYK1 gene. This system had two components designed to

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2

Quan, Zhenzhen. "Regulation of starvation-induced gene expression in Saccharomyces cerevisiae." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610892.

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3

Zaman, Zafar. "Regulation of expression of the LPD1 gene in Saccharomyces cerevisiae." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/11671.

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The <i>LPD1</i> gene of <i>Saccharomyces cerevisiae</i> encoding lipoamide dehydrogenase (LPDH) has been shown to be subject to the general control of amino acid biosynthesis mediated via the <i>CGN4</i> gene product. It is subject to catabolite repression and was shown to require the <i>HAP2, HAP3</i> andf <i>HAP4</i> gene products for release from glucose repression. The gene also appears to contain a carbon source-regulated transcriptional enhancer that lies 3' to the translational start site. A defined set of isogenic yeast strains was constructed in which each strain contained a different

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4

Mojzita, Dominik. "Thiamine-related regulation of metabolism and gene expression in the yeast Saccharomyces cerevisiae /." Göteborg : Dept. of Cellular and Molecular Biology, Göteborg University, 2007. http://www.loc.gov/catdir/toc/fy0804/2007440827.html.

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5

Smith, Erin N. "Gene-environment interaction in yeast gene expression /." Thesis, Connect to this title online; UW restricted, 2008. http://hdl.handle.net/1773/5025.

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6

Kleinschmidt, Malte. "Regulation of gene expression and adhesion in Saccharomyces cerevisiae." Doctoral thesis, [S.l.] : [s.n.], 2005. http://webdoc.sub.gwdg.de/diss/2005/kleinschmidt.

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7

Valerius, Oliver. "Chromatin structure and regulation of gene expression at the histidine-adenine branch point in yeast and aspergillus." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963077031.

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8

Van, der Merwe George K. (George Karel)1968. "NCR-sensitive gene expression and regulation of nitrogen interconversion by VID30 in Saccharomyces cerevisiae." Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52952.

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Dissertation (PhD)--University of Stellenbosch, 2002.<br>ENGLISH ABSTRACT: Saccharomyces cerevisiae uses the nitrogenous compounds in its environment selectively. The basis of this phenomenon is the transcriptional regulation of genes whose products are required for nitrogen catabolism. A rich nitrogen source represses the expression of genes required for the degradation of poor nitrogen sources via the action of the target of rapamyein (TOR) signaling cascade. If only a poor nitrogen source is available, these genes are derepressed. This process is known as nitrogen catabolite repressi

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9

Richardson, Jonathan Paul. "Post-transcriptional regulation of gene expression by the (PSI) prion of Saccharomyces cerevisiae." Thesis, University of Aberdeen, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395144.

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Termination of protein synthesis in the yeast <I>Saccharomyces cerevisiae </I>occurs when the eukaryotic release factor eRF1 recognises the stop codon. The rate of termination is enhanced by a second release factor, eRF3 (a GTPase). The efficiency of stop codon recognition by eRF1 is influenced by the surrounding nucleotide context of the stop codon, and in yeast, by the structural properties of eRF3, which displays prion-like characteristics. eRF3 in the [<I>PSI</I><sup>+</sup>], prion state forms insoluble high molecular weight aggregates that cause inefficient termination (nonsense suppress

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10

Louw, Campbell Trout. "Transcriptional regulation of the endo-polygalacturonase-encoding gene in Saccharomyces cerevisiae." Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/4005.

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Thesis (PhD (Science) (Viticulture and Oenology. Wine Biotechnology))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: Wine fermentation with a yeast strain able to degrade grape cell polysaccharides can result in improved processability and an increase in wine quality by improving extraction of essential compounds from the grapes during the maceration stage. Pectin is the only important cell wall polysaccharide that can be degraded by wild-type Saccharomyces cerevisiae strains. Pectin is degraded by a polygalacturonase (PG) encoded by the PGU1 gene (ORF YJR153W). Only certain S. c

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11

Wiley, Emily A. "Yeast telomere structure : genetic analysis implicating a novel terminus-specific factor in telomeric silencing /." Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/6359.

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12

Brewster, Neil Kenneth. "Studies on the regulation of pyruvate carboxylase isozyme (PYC1 PYC2) gene expression in Saccharomyces cerevisiae /." Title page, abstract and contents only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phb848.pdf.

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13

Tsujimoto, Yoshiyuki. "Regulation of DOG2 Gene Expression and Signal Transduction in Environmental Stress Response of saccharomyces cerevisiae." Kyoto University, 1998. http://hdl.handle.net/2433/157117.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである<br>Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第7406号<br>農博第990号<br>新制||農||762(附属図書館)<br>学位論文||H10||N3152(農学部図書室)<br>UT51-98-G335<br>京都大学大学院農学研究科食品工学専攻<br>(主査)教授木村光, 教授天知輝夫, 教授江﨑信芳<br>学位規則第4条第1項該当

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14

Santo, Julio Cezar Araujo do Espírito. "Aperfeiçoamento da fermentação de sacarose através da modificação da expressão dos genes SUC2 e AGT1 em linhagens diploides de Saccharomyces cerevisiae." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04062012-112721/.

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Atualmente, as cepas de S. cerevisiae utilizadas na produção industrial de álcool combustível no Brasil são leveduras diplóides que metabolizam a sacarose através da sua hidrolise extracelular pela invertase periplasmática, seguida pelo transporte para o interior da célula das moléculas de glicose e frutose formadas, e posterior metabolização pela glicólise. Neste trabalho, utilizando técnicas de engenharia genética e posteriores cruzamentos, foram desenvolvidas cepas diplóides de S. cerevisiae capazes de consumir este açúcar por meio da sua captação direta pelo transportador codificado pelo g

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15

Guo, Yingying. "Characterization of global transcriptional responses and DNA repair following aflatoxin B₁ treatment in Saccharomyces cerevisiae /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/8478.

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16

Béve, Jenny. "Structure and function of the yeast mediator tail domain /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-599-2/.

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17

Lenardon, Megan Denise Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "The role of auxiliary transcription factors in the regulation of gene expression during sporulation in Saccharomyces cerevisiae." Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences, 2005. http://handle.unsw.edu.au/1959.4/20818.

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Sporulation in Saccharomyces cerevisiae includes the processes of meiosis and spore formation. The genes involved in this developmental process are tightly regulated at the level of transcription to ensure that genes are expressed at the correct time and level. The co-ordinated expression of middle sporulation genes is mediated by a key timing promoter element called the middle sporulation element (MSE). While this element sets the timing of gene expression to middle sporulation, in some cases, the level of expression is mediated by cis-acting auxiliary promoter elements. This study has ad

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18

Ronsmans, Aria. "Mechanisms of nitrogen catabolite repression-sensitive gene regulation by the GATA transcription factors in Saccharomyces cerevisiae." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209169.

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The process of specific gene transcription by RNA polymerase II (Pol II) is initiated by the<p>binding of specific transcription factors to DNA. A global understanding of the mechanisms of gene<p>transcriptional regulation of Saccharomyces cerevisiae goes through the description of the targets and<p>the behavior of those transcription factors.<p>The GATA factors are specific transcription factors intervening in the regulation of Nitrogen<p>Catabolite Repression (NCR)-sensitive genes, a mechanism encompassing the transcriptional<p>regulations leading to the preferential use of good nitrogen sou

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19

Enjalbert, Brice. "Régulation transcriptionnelle de Gsy2p, glycogène synthase majeure de la levure Saccharomyces cerevisiae." Toulouse, INSA, 2001. http://www.theses.fr/2001ISAT0003.

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La levure Saccharomyces cerevisiae se doit de répondre aux stress et contraintes environnementales pour assurer sa survie et sa prolifération. Un de ces mécanismes de réponse adaptative est l'accumulation de sucres de réserve comme le glycogène lors d'une limitation nutritionnelle. Nous avons démontré que l'ensemble des protagonistes impliqués dans le métabolisme du glycogène est induit en un même stimulon par un phénomène d'induction transcriptionnelle à la sortie de la phase exponentielle de croissance sur glucose. La majorité de ces gènes possède des éléments STREs (STress Response Elements

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20

Nigavekar, Shraddha S. "Regulation of GLC7 encoded PP1 and analysis of synthetic lethal interactions with ade3 and leu2 in saccharomyces cerevisiae." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3013007.

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21

Boban, Mirta. "Transcriptional regulation by inner nuclear membrane proteins /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-170-8/.

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22

Yu, Josephine V. "Characterization of the alcohol dehydrogenase II regulatory sequences in yeast Saacharomyces cerevisae /." Thesis, Connect to this title online; UW restricted, 1989. http://hdl.handle.net/1773/9267.

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23

Apone, Lynne Marie. "Analysis of TAF II Function in the Yeast Saccharomyces Cerevisiae." eScholarship@UMMS, 1998. https://escholarship.umassmed.edu/gsbs_diss/183.

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Transcription by RNA polymerase II is a highly regulated process requiring a number of general and promoter specific transcription factors. Although many of the factors involved in the transcription reaction are known, exactly how they function to stimulate or repress transcription is not well understood. Central to understanding gene regulation is understanding the mechanism by which promoter specific transcription activators (activators) stimulate transcription. A group of factors called coactivators have been shown to be required for activator function in vitro. The best characterized coact

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24

Fazzio, Thomas G. "Isw2 complex slides nucleosomes to create repressive chromatin structure in vivo /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/5092.

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25

Drolet, Jessica Ann. "Evidence for the involvement of the zinc cluster protein Asg1p in the transcriptional regulation of some stress response genes in Saccharomyces cerevisiae." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=112617.

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Saccharomyces cerevisiae has developed mechanisms in order to survive harsh environmental conditions. This species responds to stresses such as ethanol, heat, and weak acid exposure via two well-characterized stress response pathways. These typically involve either the Hsf1p or the Msn2/4p transcriptional regulators. Recently, our lab has begun to characterize a member of the zinc cluster protein family: Asg1p (Activator of Stress Genes, systematic name: YIL130W), which is presumed to stimulate stress response genes independently of the Hsflp and Msn2/4p pathways. Previous work has revealed fi

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26

Miele, Adriana. "Analysis of Long-Range Chromosomal Interactions in Saccharomyces cerevisiae: A Dissertation." eScholarship@UMMS, 2009. https://escholarship.umassmed.edu/gsbs_diss/413.

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Long-range chromosomal interactions have been discovered in a number of organisms, suggesting that gene regulation through direct physical association with regulatory elements and/or other genes is a common and conserved phenomenon. This thesis investigates the relationship between direct physical contact of genomic loci and how these interactions may play a role in gene regulation. Analysis of such levels of chromosomal organization has been made possible in part by the emergence of Chromosome Conformation Capture (3C). This technique makes use of formaldehyde crosslinking to trap interacting

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27

Chen, Hsiuyi V. "Systematic Dissection of Roles for Chromatin Regulators in Dynamics of Transcriptional Response to Stress in Yeast: A Dissertation." eScholarship@UMMS, 2015. http://escholarship.umassmed.edu/gsbs_diss/808.

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The following work demonstrates that chromatin regulators play far more pronounced roles in dynamic gene expression than they do in steady-state. Histone modifications have been associated with transcription activity. However, previous analyses of gene expression in mutants affecting histone modifications show limited alteration. I systematically dissected the effects of 83 histone mutants and 119 gene deletion mutants on gene induction/repression in response to diamide stress in yeast. Importantly, I observed far more changes in gene induction/repression than changes in steady-state gene expr

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28

Shaikhibrahim, Zaki. "Dynamics of protein folding and subunit interactions in assembly of the yeast mediator complex." Doctoral thesis, Umeå : Umeå university, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-29976.

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29

Montigny, Jacky de. "Ura5 et ura10, deux genes codant pour deux isoenzymes a activite omp pyrophosphorylase chez la levure saccharomyces cerevisiae : structure, expression et regulation." Strasbourg 1, 1988. http://www.theses.fr/1988STR13198.

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30

Paulovich, Amanda G. "The regulation of S phase progression rate in yeast in response to DNA damage /." Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/10263.

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31

Lavoie, Mathieu. "Étude des mécanismes de dégradation sélective de l’ARN par la RNase III de Saccharomyces cerevisiae." Thèse, Université de Sherbrooke, 2014. http://hdl.handle.net/11143/5999.

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Résumé : Chez toutes les cellules, une modulation précise de l’expression des gènes est essentielle afin de réguler adéquatement leur métabolisme et de s’adapter aux changements environnementaux. En effet, c’est l’expression des gènes, plutôt que la séquence d’ADN, qui détermine en grande partie la diversité et la complexité des organismes. Celle-ci dépend principalement des changements dans les niveaux d’ARNs cellulaires résultant de la modification de l’équilibre entre leurs taux relatifs de synthèse et de dégradation. Alors que la régulation transcriptionnelle a été largement étudiée par le

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32

Potier, Serge. "Translocation reciproque entre sites chromosomiques choisis : remplacement du locus ura2 sauvage par des alleles deletes in vitro chez saccharomyces cerevisiae." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13121.

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Etude fine du gene ura2 grace aux techniques d'integration chromosomique ou loeus ou remplacement genique et analyse de l'expression, la regulation du gene et du fonctionnement de l'enzyme bifonctionnel codee pae ce gene. Mise au point d'une methode permettant de faire des translocations reciproques stables entre 2 sites choisis

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33

Kundu, Sharmistha. "Chromatin Remodeling and Transcriptional Memory: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/408.

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Transcriptional regulation of gene expression is critical for all unicellular and multicellular organisms. The ability to selectively induce or repress expression of only a few genes from the entire genome gives cells the ability to respond to changing environmental conditions, grow and proliferate. Multicellular organisms begin life as a single totipotent cell, which undergoes many cell divisions during embryonic and later postnatal development. During this process, the dividing cells of the embryo progressively lose their pluripotency and adopt restricted cell fates. Cell fate restriction le

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34

Teufel, Lotte. "Cyclins and their roles in cell cycle progression, transcriptional regulation and osmostress adaptation in Saccharomyces cerevisiae. A transcriptome-wide and single cell approach." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21205.

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Der eukaryotische Zellzyklus ist ein streng regulierter Prozess, für dessen zeitlichen Ablauf unter anderem oszillierende Genexpression notwendig ist. Die Regulation und die zeitliche Koordination des Zellzyklus sind nach wie vor fundamentale Fragen der Zellbiologie. Spezifische Ereignisse, wie DNA Replikation und Zellkernteilung, können vier Zellzyklusphasen zugeordnet werden, welche durch Cyclin-abhängige Kinasen, Cycline und deren Inhibitoren reguliert werden. Während in Saccharomyces cerevisiae Cyclin-abhängige Kinasen (Cdc28, Pho85) über den gesamten Zellzyklus zu Verfügung stehen, wer

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35

Chang, Chien-I. "Functional Analysis of Yeast Pheromone Receptors in ER Exit, Ligand-Induced Endocytosis and Oligomerization: A Dissertation." eScholarship@UMMS, 2009. https://escholarship.umassmed.edu/gsbs_diss/418.

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This study investigates endocytosis and ER export signals of the yeast α-factor receptor and the role that receptor oligomerization plays in these processes. The α-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, I found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the α-factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays strongly suggested that ligand-induced co

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36

Hatton, Lee S. "Gluconeogenic gene regulation in Saccharomyces cerevisiae." Thesis, University of Aberdeen, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387524.

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The yeast <I>FBP1 </I>and <I>PCK1</I> genes and the gluconeogenic enzymes that they encode, fructose-1,6-<I>bis</I>phosphatase and phosphoenolpyruvate carboxykinase, are subject to multiple levels of regulation by glucose. It has been reported that transcriptional repression of these genes is exceptionally sensitive to glucose, being triggered by glucose concentrations of less than 0.005% (0.25 mM). It was shown here that in addition at transcriptional repression, the <I>FBP1 </I>and <I>PCK1</I> and mRNAs are destabilised about 2-fold upon addition of the same low levels of glucose. Low levels

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37

Summers, Ryan Michael. "Gene Expression Analysis of Immobilized Saccharomyces Cerevisiae." DigitalCommons@USU, 2008. https://digitalcommons.usu.edu/etd/68.

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Immobilization is an effective method to increase ethanol production, as proven by previous research. Results almost exclusively demonstrate an increase in ethanol production by and decrease in reproduction rate of immobilized Saccharomyces cerevisiae cells. Recently, research has been conducted to determine the cause of this change. The extreme variance in results due to lack of technology makes it difficult to determine the cellular changes induced by immobilization. With the advent of new technology, specifically gene expression analysis, the RNA content of cells can be easily and rapid

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38

Tournu, Helene. "Global analyses of gene expression in Saccharomyces cerevisiae." Thesis, University of Aberdeen, 2001. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU535196.

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As part of the EUROFAN Transcript Consortium, northern analysis was used to study the expression of 200 yeast orphan genes. Five transient growth conditions were analysed, including glucose upshift, stationary phase, nitrogen starvation, osmotic shock, and heat shock. Around 72% of the genes analysed were expressed at low level, about 12% were expressed at a medium level, and 3.5% at a high level relative to the ACT1 mRNA. Almost half of the genes were regulated significantly in response to at least one of the growth conditions. Expression levels did not correlate with the Codon Adaptation Ind

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39

Mastrangelo, Peter. "Transcription regulation of the Saccharomyces cerevisiae actin gene." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ27461.pdf.

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Zheng, Yun. "Regulation of the GCV3 gene in Saccharomyces cerevisiae." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0015/MQ47798.pdf.

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41

Liu, Yuchen. "Meiotic ncRNA expression and regulation in saccharomyces cerevisiae." Rennes 1, 2011. http://www.theses.fr/2011REN1S039.

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Pervasively transcribed mitotic ncRNAs were described in budding yeast, but little is known during meiotic development. We carried out a tiling array analyzing the transcriptome of budding yeast during whole time course of meiotic development and spore formation, and a non synchronized fermenting and respiring cell culture were also studied. Transcripts that are differentially expressed or specifically transcribed during sporulation including meiotic unannotated transcripts (MUTs), and meiotic specific untranslated regions (mUTRs) were identified. MUTs are mitotic targets of conserved nuclear

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42

Shukla, Abhijit. "HISTONE POSTTRANSLATIONAL MODIFICATIONS AND GENE EXPRESSION IN SACCHAROMYCES CEREVISIAE." Available to subscribers only, 2009. http://proquest.umi.com/pqdweb?did=1967969411&sid=3&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Thesis (Ph. D.)--Southern Illinois University Carbondale, 2009.<br>"Department of Molecular Biology, Microbiology and Biochemistry." Includes bibliographical references (p. 131-155). Also available online.

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Miller, Christian. "Gene regulation during stress response transcription in Saccharomyces Cerevisiae." Diss., lmu, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-155087.

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44

Dickson, Lorna Mary. "Translation during growth and starvation in Saccharomyces cerevisiae." Thesis, University of Aberdeen, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320770.

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The translation of a series of <I>cat </I>mRNAs containing either the <I>HSP26 </I>5'- leader or various artificial 5'-leaders (Vega Laso <I>et al., </I>1993) were analysed during growth. From this study, the relative translational efficiencies of these mRNAs were shown to vary from 2% to 100% during mid-exponential phase as observed previously (Vega Laso <I>et al.,</I>1993). However, upon analysing the translation of the various <I>cat </I>constructs during growth, their relative translational efficiencies did not change significantly as yeast cells approached stationary phase. A new set of <

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45

Chacko, Alexander D. "Post-transcriptional control of gluconeogenic gene expression in Saccharomyces cerevisiae." Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301079.

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This thesis examines the post-transcriptional regulation of the gluconeogenic mRNAs <I>PCK1</I> and <I>FBP1</I> in <I>Saccharomyces cerevisiae</I>. By construction of a set of chimaeric <I>PCK1-PGK1</I> reporter genes, it was possible to show that sequences from the 5' end of the <I>PCK1</I> mRNA were capable of conferring post-transcriptional glucose regulation on the reporter. These regions were presumably the ultimate target of the glucose signal on the wild-type <I>PCK1</I> mRNA. Sequences from the 5' end of the <I>SDH2</I> mRNA had previously been shown to be required for the rapid degrad

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46

Rathjen, Joy. "Expression signals for the phosphoglycerate kinase gene of saccharomyces cerevisiae." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670320.

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47

Jordan, Bernadette. "Factors influencing heterologous gene expression in the yeast Saccharomyces cerevisiae." Thesis, University of Leicester, 1993. http://hdl.handle.net/2381/34432.

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The yeast Saccharomyces cerevisiae is widely employed as a host for the production of recombinant proteins. In the work presented here, expression of recombinant reporter proteins has been analysed in yeast, with the aim of determining factors that contribute to the expression performance of recombinant genes. Initially, expression of bacterial aminoglycoside phosphotransferase was analysed in yeast. This heterologous gene showed very low expression levels, resulting from a small decrease in mRNA abundance, plus a large decrease in translation efficiency. This appeared to be due to an unfavour

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48

McLaren, Niall Ferguson. "An investigation of heavy metal gene regulation in Saccharomyces cerevisiae." Thesis, Heriot-Watt University, 2003. http://hdl.handle.net/10399/382.

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49

Ross, Joseph. "Regulation of the gene encoding lipoamide dehydrogenase in Saccharomyces cerevisiae." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/27307.

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Drobiak, Stephanie L. "Characterization of the Zps1p cell wall protein from saccharomyces cerevisiae." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0008961.

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