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Journal articles on the topic 'Saccharomyces cerevisiae Gene Expression Regulation'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Zitomer, R. S., and C. V. Lowry. "Regulation of gene expression by oxygen in Saccharomyces cerevisiae." Microbiological Reviews 56, no. 1 (1992): 1–11. http://dx.doi.org/10.1128/mmbr.56.1.1-11.1992.

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Zitomer, R. S., and C. V. Lowry. "Regulation of gene expression by oxygen in Saccharomyces cerevisiae." Microbiological Reviews 56, no. 1 (1992): 1–11. http://dx.doi.org/10.1128/mr.56.1.1-11.1992.

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3

Lapinskas, Paula, Helmut Ruis, and Valeria Culotta. "Regulation of Saccharomyces cerevisiae catalase gene expression by copper." Current Genetics 24, no. 5 (1993): 388–93. http://dx.doi.org/10.1007/bf00351846.

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4

Tadi, D., E. Boy-Marcotte, H. Boucherie, and M. Jacquet. "Regulation of gene expression by cAMP in saccharomyces cerevisiae." Biology of the Cell 84, no. 1-2 (1995): 121. http://dx.doi.org/10.1016/0248-4900(96)81472-9.

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5

Nagahashi, S., H. Nakayama, K. Hamada, H. Yang, M. Arisawa, and K. Kitada. "Regulation by tetracycline of gene expression in Saccharomyces cerevisiae." Molecular and General Genetics MGG 255, no. 4 (1997): 372–75. http://dx.doi.org/10.1007/s004380050508.

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6

Kochenova, Olga V. "Noncoding RNA participation in gene expression regulation in yeast Saccharomyces cerevisiae." Ecological genetics 9, no. 1 (2011): 3–14. http://dx.doi.org/10.17816/ecogen913-14.

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Saccharomyces cerevisiae lacks the main components of RNAi-dependent gene silencing. Nevertheless, regulation of gene expression in S. cerevisiae could be accomplished via some other types of noncoding RNA, particularly via antisense RNA. Although, there is a high percent of untranslated RNA in yeast genome only few evidences of noncoding RNA gene regulation exist in yeast S. cerevisiae, some of them are reviewed in the present paper.

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Wiles, Amy M., Houjian Cai, Fred Naider, and Jeffrey M. Becker. "Nutrient regulation of oligopeptide transport in Saccharomyces cerevisiae." Microbiology 152, no. 10 (2006): 3133–45. http://dx.doi.org/10.1099/mic.0.29055-0.

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Small peptides (2–5 amino acid residues) are transported into Saccharomyces cerevisiae via two transport systems: PTR (Peptide TRansport) for di-/tripeptides and OPT (OligoPeptide Transport) for oligopeptides of 4–5 amino acids in length. Although regulation of the PTR system has been studied in some detail, neither the regulation of the OPT family nor the environmental conditions under which family members are normally expressed have been well studied in S. cerevisiae. Using a lacZ reporter gene construct fused to 1 kb DNA from upstream of the genes OPT1 and OPT2, which encode the two S. cere

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Kawaguchi, Hiroko, Manabu Yoshida, and Ichiro Yamashita. "Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae." Bioscience, Biotechnology, and Biochemistry 56, no. 2 (1992): 289–97. http://dx.doi.org/10.1271/bbb.56.289.

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Susek, R. E., and S. Lindquist. "Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock." Molecular and Cellular Biology 10, no. 12 (1990): 6362–73. http://dx.doi.org/10.1128/mcb.10.12.6362.

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hsp26, the small heat shock protein of Saccharomyces cerevisiae, accumulates in response to heat and other types of stress. It also accumulates during the normal course of development, as cells enter stationary phase growth or begin to sporulate (S. Kurtz, J. Rossi, L. Petko, and S. Lindquist, Science 231:1154-1157, 1986). Analysis of deletion and insertion mutations demonstrated that transcriptional control plays a critical role in regulating HSP26 expression. The HSP26 promoter was found to be complex and appears to contain repressing elements as well as activating elements. Several upstream

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Susek, R. E., and S. Lindquist. "Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock." Molecular and Cellular Biology 10, no. 12 (1990): 6362–73. http://dx.doi.org/10.1128/mcb.10.12.6362-6373.1990.

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hsp26, the small heat shock protein of Saccharomyces cerevisiae, accumulates in response to heat and other types of stress. It also accumulates during the normal course of development, as cells enter stationary phase growth or begin to sporulate (S. Kurtz, J. Rossi, L. Petko, and S. Lindquist, Science 231:1154-1157, 1986). Analysis of deletion and insertion mutations demonstrated that transcriptional control plays a critical role in regulating HSP26 expression. The HSP26 promoter was found to be complex and appears to contain repressing elements as well as activating elements. Several upstream

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11

Zitomer, R. S., J. W. Sellers, D. W. McCarter, G. A. Hastings, P. Wick, and C. V. Lowry. "Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene." Molecular and Cellular Biology 7, no. 6 (1987): 2212–20. http://dx.doi.org/10.1128/mcb.7.6.2212.

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The CYC7 gene of Saccharomyces cerevisiae encodes the minor species, iso-2, of the cytochrome c protein. Its expression is governed by two regulatory sequences upstream from the gene: a positive site which stimulates transcription 240 base pairs 5' from the protein-coding sequence (-240) and a negative site which inhibits transcription at -300. In this study, the nature of the positive site and its relationship to the negative site has been investigated. Expression of the CYC7 gene is weakly inducible by oxygen. This effect was greatly enhanced by the semidominant CYP1-16 mutation in the trans

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12

Zitomer, R. S., J. W. Sellers, D. W. McCarter, G. A. Hastings, P. Wick, and C. V. Lowry. "Elements involved in oxygen regulation of the Saccharomyces cerevisiae CYC7 gene." Molecular and Cellular Biology 7, no. 6 (1987): 2212–20. http://dx.doi.org/10.1128/mcb.7.6.2212-2220.1987.

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The CYC7 gene of Saccharomyces cerevisiae encodes the minor species, iso-2, of the cytochrome c protein. Its expression is governed by two regulatory sequences upstream from the gene: a positive site which stimulates transcription 240 base pairs 5' from the protein-coding sequence (-240) and a negative site which inhibits transcription at -300. In this study, the nature of the positive site and its relationship to the negative site has been investigated. Expression of the CYC7 gene is weakly inducible by oxygen. This effect was greatly enhanced by the semidominant CYP1-16 mutation in the trans

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13

Forlani, Nicoletta, Enzo Martegani, and Lilia Alberghina. "Posttranscriptional regulation of the expression of MET2 gene of Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1089, no. 1 (1991): 47–53. http://dx.doi.org/10.1016/0167-4781(91)90083-x.

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KING, LORRAINE, and GERALDINE BUTLER. "Regulation of expression of the chitinase gene CTS1 in Saccharomyces cerevisiae." Biochemical Society Transactions 25, no. 3 (1997): 555S. http://dx.doi.org/10.1042/bst025555s.

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15

Jordá, Tania, and Sergi Puig. "Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae." Genes 11, no. 7 (2020): 795. http://dx.doi.org/10.3390/genes11070795.

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Ergosterol is an essential component of fungal cell membranes that determines the fluidity, permeability and activity of membrane-associated proteins. Ergosterol biosynthesis is a complex and highly energy-consuming pathway that involves the participation of many enzymes. Deficiencies in sterol biosynthesis cause pleiotropic defects that limit cellular proliferation and adaptation to stress. Thereby, fungal ergosterol levels are tightly controlled by the bioavailability of particular metabolites (e.g., sterols, oxygen and iron) and environmental conditions. The regulation of ergosterol synthes

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Smith, H. E., S. S. Su, L. Neigeborn, S. E. Driscoll, and A. P. Mitchell. "Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 12 (1990): 6103–13. http://dx.doi.org/10.1128/mcb.10.12.6103.

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Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-specific gene expression. We report here the sequence of the IME1 gene and the consequences of IME1 expression from the GAL1 promoter. The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region. Expression of PGAL1-IME1 in vegetative a/alpha cells led

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Smith, H. E., S. S. Su, L. Neigeborn, S. E. Driscoll, and A. P. Mitchell. "Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 12 (1990): 6103–13. http://dx.doi.org/10.1128/mcb.10.12.6103-6113.1990.

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Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-specific gene expression. We report here the sequence of the IME1 gene and the consequences of IME1 expression from the GAL1 promoter. The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region. Expression of PGAL1-IME1 in vegetative a/alpha cells led

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18

Miyata, Non, Takuya Miyoshi, Takanori Yamaguchi, Toshimitsu Nakazono, Motohiro Tani, and Osamu Kuge. "VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae." Biochemical Journal 472, no. 3 (2015): 319–28. http://dx.doi.org/10.1042/bj20150884.

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Regulation of expression of the PS decarboxylase 2 (PSD2) gene in Saccharomyces cerevisiae is poorly understood. We found that deletion of VID22 resulted in a decrease in the activity of the Psd2p enzyme through down-regulation of PSD2 gene expression.

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19

Iraqui, Ismaïl, Stéphan Vissers, Bruno André, and Antonio Urrestarazu. "Transcriptional Induction by Aromatic Amino Acids in Saccharomyces cerevisiae." Molecular and Cellular Biology 19, no. 5 (1999): 3360–71. http://dx.doi.org/10.1128/mcb.19.5.3360.

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ABSTRACT Aromatic aminotransferase II, product of the ARO9 gene, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism in Saccharomyces cerevisiae. ARO9 expression is under the dual control of specific induction and nitrogen source regulation. We have here identified UASaro, a 36-bp upstream element necessary and sufficient to promote transcriptional induction of reporter gene expression in response to tryptophan, phenylalanine, or tyrosine. We then isolated mutants in which UASaro-mediated ARO9 transcription is partially or totally impaired. Mutations abolishingARO9 i

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20

Smith, S. J., J. H. Crowley, and L. W. Parks. "Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 16, no. 10 (1996): 5427–32. http://dx.doi.org/10.1128/mcb.16.10.5427.

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Sterol biosynthesis in the yeast Saccharomyces cerevisiae is an energy-expensive, aerobic process, requiring heme and molecular oxygen. Heme, also synthesized exclusively during aerobic growth, not only acts as an enzymatic cofactor but also is directly and indirectly responsible for the transcriptional control of several yeast genes. Because of their biosynthetic similarities, we hypothesized that ergosterol, like heme, may have a regulatory function. Sterols are known to play a structural role in membrane integrity, but regulatory roles have not been characterized. To test possible regulator

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21

Tang, Hongting, Yanling Wu, Jiliang Deng, et al. "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae." Metabolites 10, no. 8 (2020): 320. http://dx.doi.org/10.3390/metabo10080320.

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Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However, native promoters in S. cerevisiae have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances

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22

Boorstein, W. R., and E. A. Craig. "Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 6 (1990): 3262–67. http://dx.doi.org/10.1128/mcb.10.6.3262.

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The SSA3 gene of Saccharomyces cerevisiae, a member of the HSP70 multigene family, is expressed at low levels under optimal growth conditions and is dramatically induced in response to heat shock. Sequences coinciding with two overlapping heat shock elements, located 156 base pairs upstream of the transcribed region, were necessary and sufficient for regulation of heat induction. The SSA3 promoter was also activated in an ssa1ssa2 double-mutant strain. This increase in the expression of SSA3 was mediated via the same upstream activating sequences that activated transcription in response to hea

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Boorstein, W. R., and E. A. Craig. "Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 6 (1990): 3262–67. http://dx.doi.org/10.1128/mcb.10.6.3262-3267.1990.

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The SSA3 gene of Saccharomyces cerevisiae, a member of the HSP70 multigene family, is expressed at low levels under optimal growth conditions and is dramatically induced in response to heat shock. Sequences coinciding with two overlapping heat shock elements, located 156 base pairs upstream of the transcribed region, were necessary and sufficient for regulation of heat induction. The SSA3 promoter was also activated in an ssa1ssa2 double-mutant strain. This increase in the expression of SSA3 was mediated via the same upstream activating sequences that activated transcription in response to hea

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24

Werner-Washburne, Margaret, and Elizabeth A. Craig. "Expression of members of the Saccharomyces cerevisiae hsp70 multigene family." Genome 31, no. 2 (1989): 684–89. http://dx.doi.org/10.1139/g89-125.

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The hsp70 multigene family of Saccharomyces cerevisiae is a complex multigene family, composed of members exhibiting complex patterns of regulation. Expression of some members is induced after a heat shock, whereas expression of others is repressed. Some members of the family are expressed during exponential growth. One gene, SSA3, shows an unusual pattern of expression during approach to stationary phase. While most RNAs decrease in abundance, SSA3 RNA levels dramatically increase. The constitutive expression of SSA3 in cells lacking adenylate cyclase activity suggests that cAMP modulates SSA

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Jiang, Y. W., and D. J. Stillman. "Regulation of HIS4 expression by the Saccharomyces cerevisiae SIN4 transcriptional regulator." Genetics 140, no. 1 (1995): 103–14. http://dx.doi.org/10.1093/genetics/140.1.103.

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Abstract The yeast SIN4 gene functions in the transcriptional activation and repression of diverse yeast genes. Previous experiments suggest a sin4 mutation affects chromatin structure and thus alters transcriptional regulation. In this report we show that SIN4 is required for full expression of the HIS4, Ty1, and MAT alpha genes, in addition to the previously described SIN4-dependence of CTS1 expression. All of these genes contain within their promoters a binding site for the Rap1p transcriptional regulator. However, SIN4 does not play a direct role either in transcriptional activation or rep

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Lemire, J. M., T. Willcocks, H. O. Halvorson, and K. A. Bostian. "Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 8 (1985): 2131–41. http://dx.doi.org/10.1128/mcb.5.8.2131.

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We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in Saccharomyces cerevisiae at the molecular level by analysis of previously isolated and genetically well-defined regulatory gene mutants known to affect APase expression. These mutants identify numerous positive- (PHO4, PHO2, PHO81) and negative-acting (PHO80, PHO85) regulatory loci dispersed throughout the yeast genome. We showed that the interplay of these positive and negative regulatory genes occurs before

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Lemire, J. M., T. Willcocks, H. O. Halvorson, and K. A. Bostian. "Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 8 (1985): 2131–41. http://dx.doi.org/10.1128/mcb.5.8.2131-2141.1985.

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We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in Saccharomyces cerevisiae at the molecular level by analysis of previously isolated and genetically well-defined regulatory gene mutants known to affect APase expression. These mutants identify numerous positive- (PHO4, PHO2, PHO81) and negative-acting (PHO80, PHO85) regulatory loci dispersed throughout the yeast genome. We showed that the interplay of these positive and negative regulatory genes occurs before

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Ferré, P. "Regulation of gene expression by glucose." Proceedings of the Nutrition Society 58, no. 3 (1999): 621–23. http://dx.doi.org/10.1017/s0029665199000816.

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Fatty acid synthase (EC 2.3.1.85) is an enzyme involved in the lipogenic pathway allowing fatty acid synthesis from glucose. Glucose up-regulates the transcription of the fatty acid synthase gene in both adipocytes and hepatocytes, with insulin having only an indirect role. The signal metabolite could be glucose-6-phosphate rather than glucose itself. The glucose response element of the fatty acid synthase gene has not yet been precisely identified, although a −2 kb region of the fatty acid synthase promoter is sufficient to confer nutritional responsiveness to a reporter gene. ADD1/SREBP1, a

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Slater, M. R., and E. A. Craig. "Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 5 (1987): 1906–16. http://dx.doi.org/10.1128/mcb.7.5.1906.

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The yeast Saccharomyces cerevisiae contains three heat-inducible hsp70 genes. We have characterized the promoter region of the hsp70 heat shock gene YG100, that also displays a basal level of expression. Deletion of the distal region of the promoter resulted in an 80% drop in the basal level of expression without affecting expression after heat shock. Progressive-deletion analysis suggested that sequences necessary for heat-inducible expression are more proximal, within 233 base pairs of the initiation region. The promoter region of YG100 contains multiple elements related to the Drosophila me

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Slater, M. R., and E. A. Craig. "Transcriptional regulation of an hsp70 heat shock gene in the yeast Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 5 (1987): 1906–16. http://dx.doi.org/10.1128/mcb.7.5.1906-1916.1987.

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The yeast Saccharomyces cerevisiae contains three heat-inducible hsp70 genes. We have characterized the promoter region of the hsp70 heat shock gene YG100, that also displays a basal level of expression. Deletion of the distal region of the promoter resulted in an 80% drop in the basal level of expression without affecting expression after heat shock. Progressive-deletion analysis suggested that sequences necessary for heat-inducible expression are more proximal, within 233 base pairs of the initiation region. The promoter region of YG100 contains multiple elements related to the Drosophila me

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31

Joo, Yoo Jin, Jung-Ae Kim, Joung Hee Baek, et al. "Cooperative Regulation of ADE3 Transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae." Eukaryotic Cell 8, no. 8 (2009): 1268–77. http://dx.doi.org/10.1128/ec.00116-09.

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ABSTRACT The one-carbon response regulon is essential for the biosynthesis of nucleic acids as well as several amino acids. The ADE3 gene is known to encode a crucial one-carbon regulon enzyme, tetrahydrofolate synthase, which is involved in the biosynthesis of purine and the amino acids methionine and glycine. Therefore, the mechanism through which ADE3 transcription is regulated appears to be critical for the cross-talk among these metabolic pathways. Even so, the direct involvement of ADE3 transcription through gene-specific transcription factors has not been shown clearly. In this study, t

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Kelliher, Christina M., Matthew W. Foster, Francis C. Motta, et al. "Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae." Molecular Biology of the Cell 29, no. 22 (2018): 2644–55. http://dx.doi.org/10.1091/mbc.e18-04-0255.

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In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with quantitative models suggests that a network of interconnected TFs is capable of regulating periodic genes over the cell cycle. Importantly, these dynamical models were built on transcriptomics data and assumed that TF protein levels and activity are directly correlated with mRNA abundance. To ask whether TF transcripts match protein expressio

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Thomas, D., H. Cherest, and Y. Surdin-Kerjan. "Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene." Molecular and Cellular Biology 9, no. 8 (1989): 3292–98. http://dx.doi.org/10.1128/mcb.9.8.3292.

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In Saccharomyces cerevisiae, the MET25 gene encodes O-acetylhomoserine sulfhydrylase. Synthesis of this enzyme is repressed by the presence of S-adenosylmethionine (AdoMet) in the growth medium. We identified cis elements required for MET25 expression by analyzing small deletions in the MET25 promoter region. The results revealed a regulatory region, acting as an upstream activation site, that activated transcription of MET25 in the absence of methionine or AdoMet. We found that, for the most part, repression of MET25 expression was due to a lack of activation at this site, reinforced by an in

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Thomas, D., H. Cherest, and Y. Surdin-Kerjan. "Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene." Molecular and Cellular Biology 9, no. 8 (1989): 3292–98. http://dx.doi.org/10.1128/mcb.9.8.3292-3298.1989.

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In Saccharomyces cerevisiae, the MET25 gene encodes O-acetylhomoserine sulfhydrylase. Synthesis of this enzyme is repressed by the presence of S-adenosylmethionine (AdoMet) in the growth medium. We identified cis elements required for MET25 expression by analyzing small deletions in the MET25 promoter region. The results revealed a regulatory region, acting as an upstream activation site, that activated transcription of MET25 in the absence of methionine or AdoMet. We found that, for the most part, repression of MET25 expression was due to a lack of activation at this site, reinforced by an in

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Valenzuela, Lourdes, Paola Ballario, Cristina Aranda, Patrizia Filetici, and Alicia González. "Regulation of Expression of GLT1, the Gene Encoding Glutamate Synthase in Saccharomyces cerevisiae." Journal of Bacteriology 180, no. 14 (1998): 3533–40. http://dx.doi.org/10.1128/jb.180.14.3533-3540.1998.

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ABSTRACT Saccharomyces cerevisiae glutamate synthase (GOGAT) is an oligomeric enzyme composed of three 199-kDa identical subunits encoded by GLT1. In this work, we analyzed GLT1transcriptional regulation. GLT1-lacZ fusions were prepared and GLT1 expression was determined in a GDH1wild-type strain and in a gdh1 mutant derivative grown in the presence of various nitrogen sources. Null mutants impaired inGCN4, GLN3, GAT1/NIL1, orUGA43/DAL80 were transformed with a GLT1-lacZfusion to determine whether the above-mentioned transcriptional factors had a role in GLT1 expression. A collection of increa

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de Boer, Marco, Jan-Paul Bebelman, Paula M. Goncalves, Jan Maat, Harm van Heerikhuizen, and Rudi J. Planta. "Regulation of expression of the amino acid transporter gene BAP3 in Saccharomyces cerevisiae." Molecular Microbiology 30, no. 3 (1998): 603–13. http://dx.doi.org/10.1046/j.1365-2958.1998.01094.x.

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Thomas, Dominique, and Yolande Surdin-Kerjan. "Structure of the HOM2 gene of Saccharomyces cerevisiae and regulation of its expression." Molecular and General Genetics MGG 217, no. 1 (1989): 149–54. http://dx.doi.org/10.1007/bf00330954.

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Hartig, A., J. Holly, G. Saari, and V. L. MacKay. "Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 6 (1986): 2106–14. http://dx.doi.org/10.1128/mcb.6.6.2106.

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The Saccharomyces cerevisiae STE2 gene, which is required for pheromone response and conjugation specifically in mating-type a cells, was cloned by complementation of the ste2 mutation. Transcription of STE2 is repressed by the MAT alpha 2 gene product, so that the 1.4-kilobase STE2 RNA is detected only in a or mat alpha 2 strains, not in alpha or a/alpha cells. However, STE2 RNA levels are also increased by the mating pheromone alpha-factor and decreased in strains bearing mutations in the nonspecific STE4 gene. Regulation of STE2 expression in a cells is therefore achieved by several mechani

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Hartig, A., J. Holly, G. Saari, and V. L. MacKay. "Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 6 (1986): 2106–14. http://dx.doi.org/10.1128/mcb.6.6.2106-2114.1986.

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The Saccharomyces cerevisiae STE2 gene, which is required for pheromone response and conjugation specifically in mating-type a cells, was cloned by complementation of the ste2 mutation. Transcription of STE2 is repressed by the MAT alpha 2 gene product, so that the 1.4-kilobase STE2 RNA is detected only in a or mat alpha 2 strains, not in alpha or a/alpha cells. However, STE2 RNA levels are also increased by the mating pheromone alpha-factor and decreased in strains bearing mutations in the nonspecific STE4 gene. Regulation of STE2 expression in a cells is therefore achieved by several mechani

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Hallstrom, Timothy C., David J. Katzmann, Rodrigo J. Torres, W. John Sharp, and W. Scott Moye-Rowley. "Regulation of Transcription Factor Pdr1p Function by an Hsp70 Protein in Saccharomyces cerevisiae." Molecular and Cellular Biology 18, no. 3 (1998): 1147–55. http://dx.doi.org/10.1128/mcb.18.3.1147.

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ABSTRACT Multiple or pleiotropic drug resistance in the yeastSaccharomyces cerevisiae requires the expression of several ATP binding cassette transporter-encoding genes under the control of the zinc finger-containing transcription factor Pdr1p. The ATP binding cassette transporter-encoding genes regulated by Pdr1p include PDR5 and YOR1, which are required for normal cycloheximide and oligomycin tolerances, respectively. We have isolated a new member of the PDR gene family that encodes a member of the Hsp70 family of proteins found in this organism. This gene has been designated PDR13 and is re

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Ferrando, A., S. J. Kron, G. Rios, G. R. Fink, and R. Serrano. "Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3." Molecular and Cellular Biology 15, no. 10 (1995): 5470–81. http://dx.doi.org/10.1128/mcb.15.10.5470.

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Dynamic regulation of ion transport is essential for homeostasis as cells confront changes in their environment. The gene HAL3 encodes a novel component of this regulatory circuit in the yeast Saccharomyces cerevisiae. Overexpression of HAL3 improves growth of wild-type cells exposed to toxic concentrations of sodium and lithium and suppresses the salt sensitivity conferred by mutation of the calcium-dependent protein phosphatase calcineurin. Null mutants of HAL3 display salt sensitivity. The sequence of HAL3 gives little clue to its function. However, alterations in intracellular cation conce

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42

Simchen, Giora, and Yona Kassir. "Genetic regulation of differentiation towards meiosis in the yeast Saccharomyces cerevisiae." Genome 31, no. 1 (1989): 95–99. http://dx.doi.org/10.1139/g89-018.

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Normally, meiosis and sporulation in Saccharomyces cerevisiae occur only in diploid strains and only when the cells are exposed to starvation conditions. Diploidy is determined by the mating-type system (the genes MAT, RME1, IME1), whereas the starvation signal is transmitted through the adenylate cyclase – protein kinase pathway (the genes CDC25, RAS2, CDC35 (CYR1), BCY1, TPK1, TPK2, TPK3). The two regulatory pathways converge at the gene IME1, which is a positive regulator of meiosis and whose early expression in sporulating cells correlates with the initiation of meiosis. Sites upstream (5′

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Sarokin, L., and M. Carlson. "Upstream region of the SUC2 gene confers regulated expression to a heterologous gene in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 10 (1985): 2521–26. http://dx.doi.org/10.1128/mcb.5.10.2521.

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The SUC2 gene produces two differently regulated mRNAs that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose (carbon catabolite) repression, and the 1.8-kilobase mRNA encoding intracellular invertase is synthesized constitutively. Previous work has shown that the 5' noncoding region between -650 and -418 is required for derepression of secreted invertase in response to glucose deprivation. We show here that this upstream region can confer glucose-repressible expression to a heterologous gene, a LEU2-lacZ gene fusion, that is not normally

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44

Sarokin, L., and M. Carlson. "Upstream region of the SUC2 gene confers regulated expression to a heterologous gene in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 10 (1985): 2521–26. http://dx.doi.org/10.1128/mcb.5.10.2521-2526.1985.

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The SUC2 gene produces two differently regulated mRNAs that encode two forms of invertase. The 1.9-kilobase mRNA encoding secreted invertase is regulated by glucose (carbon catabolite) repression, and the 1.8-kilobase mRNA encoding intracellular invertase is synthesized constitutively. Previous work has shown that the 5' noncoding region between -650 and -418 is required for derepression of secreted invertase in response to glucose deprivation. We show here that this upstream region can confer glucose-repressible expression to a heterologous gene, a LEU2-lacZ gene fusion, that is not normally

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Brandriss, Marjorie C. "Evidence for Positive Regulation of the Proline Utilization Pathway in Saccharomyces cerevisiae." Genetics 117, no. 3 (1987): 429–35. http://dx.doi.org/10.1093/genetics/117.3.429.

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ABSTRACT A mutation has been identified that prevents Saccharomyces cerevisiae cells from growing on proline as the sole source of nitrogen, causes noninducible expression of the PUT1 and PUT2 genes, and is completely recessive. In the put3-75 mutant, the basal level of expression (ammonia as nitrogen source) of PUT1-lacZ and PUT2-lacZ gene fusions as measured by β-galactosidase activity is reduced 4- and 7-fold, respectively, compared with the wild-type strain. Normal regulation is not restored when the cells are grown on arginine as the sole nitrogen source and put3-75 cells remain sensitive

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Marshall, M., D. Mahoney, A. Rose, J. B. Hicks, and J. R. Broach. "Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 12 (1987): 4441–52. http://dx.doi.org/10.1128/mcb.7.12.4441.

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The product of the Saccharomyces cerevisiae SIR4 gene, in conjunction with at least three other gene products, prevents expression of mating-type genes resident at loci at either end of chromosome III, but not of the same genes resident at the MAT locus in the middle of the chromosome. To address the mechanism of this novel position effect regulation, we have conducted a structural and genetic analysis of the SIR4 gene. We have determined the nucleotide sequence of the gene and found that it encodes a lysine-rich, serine-rich protein of 152 kilodaltons. Expression of the carboxy half of the pr

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47

Marshall, M., D. Mahoney, A. Rose, J. B. Hicks, and J. R. Broach. "Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae." Molecular and Cellular Biology 7, no. 12 (1987): 4441–52. http://dx.doi.org/10.1128/mcb.7.12.4441-4452.1987.

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The product of the Saccharomyces cerevisiae SIR4 gene, in conjunction with at least three other gene products, prevents expression of mating-type genes resident at loci at either end of chromosome III, but not of the same genes resident at the MAT locus in the middle of the chromosome. To address the mechanism of this novel position effect regulation, we have conducted a structural and genetic analysis of the SIR4 gene. We have determined the nucleotide sequence of the gene and found that it encodes a lysine-rich, serine-rich protein of 152 kilodaltons. Expression of the carboxy half of the pr

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Ivy, J. M., A. J. Klar, and J. B. Hicks. "Cloning and characterization of four SIR genes of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 2 (1986): 688–702. http://dx.doi.org/10.1128/mcb.6.2.688.

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Mating type in the yeast Saccharomyces cerevisiae is determined by the MAT (a or alpha) locus. HML and HMR, which usually contain copies of alpha and a mating type information, respectively, serve as donors in mating type interconversion and are under negative transcriptional control. Four trans-acting SIR (silent information regulator) loci are required for repression of transcription. A defect in any SIR gene results in expression of both HML and HMR. The four SIR genes were isolated from a genomic library by complementation of sir mutations in vivo. DNA blot analysis suggests that the four

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49

Ivy, J. M., A. J. Klar, and J. B. Hicks. "Cloning and characterization of four SIR genes of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 2 (1986): 688–702. http://dx.doi.org/10.1128/mcb.6.2.688-702.1986.

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Abstract:

Mating type in the yeast Saccharomyces cerevisiae is determined by the MAT (a or alpha) locus. HML and HMR, which usually contain copies of alpha and a mating type information, respectively, serve as donors in mating type interconversion and are under negative transcriptional control. Four trans-acting SIR (silent information regulator) loci are required for repression of transcription. A defect in any SIR gene results in expression of both HML and HMR. The four SIR genes were isolated from a genomic library by complementation of sir mutations in vivo. DNA blot analysis suggests that the four

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50

Lowry, C. V., and R. S. Zitomer. "ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae." Molecular and Cellular Biology 8, no. 11 (1988): 4651–58. http://dx.doi.org/10.1128/mcb.8.11.4651.

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The ROX1 gene encodes a product implicated in the regulation of heme-repressed and heme-induced genes in Saccharomyces cerevisiae. The gene has been cloned and shown to code for a 1.4-kilobase transcript. The cloned gene was used to construct a null mutant to determine the role of ROX1 in regulating the expression of several heme-regulated genes. Constitutive expression of ANB1 (a heme-repressed gene) was observed in the null strain, indicating that ROX1 codes for a repressor or a facilitator of repression. Enhancement of expression of CYC7 in the null strain indicated that the ROX1 factor is

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