Journal articles: 'Saccharomycetales'

1

Suh, S. O., M. Blackwell, C. P. Kurtzman, and M. A. Lachance. "Phylogenetics of Saccharomycetales, the ascomycete yeasts." Mycologia 98, no. 6 (November 1, 2006): 1006–17. http://dx.doi.org/10.3852/mycologia.98.6.1006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Suh, Sung-Oui, Meredith Blackwell, Cletus P. Kurtzman, and Marc-André Lachance. "Phylogenetics of Saccharomycetales, the ascomycete yeasts." Mycologia 98, no. 6 (November 2006): 1006–17. http://dx.doi.org/10.1080/15572536.2006.11832629.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Zhang, Wen, Xueyan Ren, Qiong Lei, and Lei Wang. "Screening and Comparison of Lignin Degradation Microbial Consortia from Wooden Antiques." Molecules 26, no. 10 (May 12, 2021): 2862. http://dx.doi.org/10.3390/molecules26102862.

Full text

Abstract:

Lignin, which is a component of wood, is difficult to degrade in nature. However, serious decay caused by microbial consortia can happen to wooden antiques during the preservation process. This study successfully screened four microbial consortia with lignin degradation capabilities (J-1, J-6, J-8 and J-15) from decayed wooden antiques. Their compositions were identified by genomic sequencing, while the degradation products were analyzed by GC-MS. The lignin degradation efficiency of J-6 reached 54% after 48 h with an initial lignin concentration of 0.5 g/L at pH 4 and rotation speed of 200 rpm. The fungal consortium of J-6 contained Saccharomycetales (98.92%) and Ascomycota (0.56%), which accounted for 31% of the total biomass. The main bacteria in J-6 were Shinella sp. (47.38%), Cupriavidus sp. (29.84%), and Bosea sp. (7.96%). The strongest degradation performance of J-6 corresponded to its composition, where Saccharomycetales likely adapted to the system and improved lignin degradation enzymes activities, and the abundant bacterial consortium accelerated lignin decomposition. Our work demonstrated the potential utilization of microbial consortia via the synergy of microbial consortia, which may overcome the shortcomings of traditional lignin biodegradation when using a single strain, and the potential use of J-6 for lignin degradation/removal applications.

APA, Harvard, Vancouver, ISO, and other styles

4

Lv, Shi-Long, Chun-Yue Chai, Yun Wang, Zhen-Li Yan, and Feng-Li Hui. "Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China." MycoKeys 75 (November 9, 2020): 31–49. http://dx.doi.org/10.3897/mycokeys.75.57192.

Full text

Abstract:

Spathaspora is an important genus of d-xylose-fermenting yeasts that are poorly studied in China. During recent yeast collections in Yunnan Province in China, 13 isolates of Spathaspora were obtained from rotting wood and all represent undescribed taxa. Based on morphological and phylogenetic analyses (ITS and nuc 28S), five new species are proposed: Spathaspora elongata, Sp. mengyangensis, Sp. jiuxiensis, Sp. parajiuxiensis and Sp. rosae. Our results indicate a high species diversity of Spathaspora waiting to be discovered in rotting wood from tropical and subtropical southwest China. In addition, the two Candida species, C. jeffriesii and C. materiae, which are members of the Spathaspora clade based on phylogeny, are transferred to Spathaspora as new combinations.

APA, Harvard, Vancouver, ISO, and other styles

5

Sakpuntoon, Varunya, Jirameth Angchuan, Wanatchaporn Boontham, Pannida Khunnamwong, Chanita Boonmak, and Nantana Srisuk. "Grease Waste as a Reservoir of Lipase-Producing Yeast and Description of Limtongella siamensis gen. nov., sp. nov." Microorganisms 8, no. 1 (December 22, 2019): 27. http://dx.doi.org/10.3390/microorganisms8010027.

Full text

Abstract:

A total of 175 yeast isolates were obtained from grease samples. Based on the D1/D2 region of the large subunit (LSU) ribosomal RNA (rRNA) gene analysis, 150 yeast isolates were identified as belonging to 36 described yeast species, whereas 25 isolates required more analysis. Among the described species, Rhodotorula mucilaginosa was the only Basidiomycetous yeast, whereas 149 isolates were identified as belonging to 35 described species of 15 genera in the phylum Ascomycota, and Candida tropicalis was the most abundant species. A study of lipase production indicated that strain DMKU-JMGT1-45 showed volumetric activity of 38.89 ± 9.62 and 155.56 ± 14.70 U/mL when grown in yeast extract malt extract (YM) and YM supplemented with 1% olive oil, respectively. In addition, this strain intracellularly accumulated lipid, of which the fatty acid profile revealed the major fatty acids to be 39.9% oleic acid (C18:1), 27.61% palmitoleic acid (C16:1) and 14.97% palmitic acid (C16:0). A phylogenetic analysis of the combined multi-locus gene sequences showed that the strains DMKU-JMGT1-45T and DMKU-JMGT4-14 formed a well-separated lineage and could not be assigned to any of the currently recognized genera of the Saccharomycetales. Limtongella siamensis gen. nov., sp. nov. is therefore proposed to accommodate these two strains as members of the order Saccharomycetales.

APA, Harvard, Vancouver, ISO, and other styles

6

de García, Virginia, Silvia Brizzio, Diego Libkind, Carlos A. Rosa, and María van Broock. "Wickerhamomyces patagonicus sp. nov., an ascomycetous yeast species from Patagonia, Argentina." International Journal of Systematic and Evolutionary Microbiology 60, no. 7 (July 1, 2010): 1693–96. http://dx.doi.org/10.1099/ijs.0.015974-0.

Full text

Abstract:

Eight strains of a novel yeast species were isolated from tree saps of ‘Coihue’ (Nothofagus dombeyi, Nothofagaceae) and glacial meltwater (Castaño Overo River) in the Nahuel Huapi National Park, Patagonia, Argentina. The sequences of the D1/D2 domains of the large subunit of the rRNA gene showed that this novel yeast species belongs to the Wickerhamomyces genus (Order Saccharomycetales, Family Wickerhamomycetaceae). The closest related species were Candida ponderosae and Wickerhamomyces chambardii. Wickerhamomyces patagonicus sp. nov. is proposed to accommodate these novel strains, with the type strain CRUB 1724T (=CBS 11398T =JCM 16381T).

APA, Harvard, Vancouver, ISO, and other styles

7

Farabaugh, Philip J., Emily Kramer, Haritha Vallabhaneni, and Ana Raman. "Evolution of +1 Programmed Frameshifting Signals and Frameshift-Regulating tRNAs in the Order Saccharomycetales." Journal of Molecular Evolution 63, no. 4 (July 12, 2006): 545–61. http://dx.doi.org/10.1007/s00239-005-0311-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Statzell-Tallman, Adele, Gloria Scorzetti, and Jack W. Fell. "Candida spencermartinsiae sp. nov., Candida taylorii sp. nov. and Pseudozyma abaconensis sp. nov., novel yeasts from mangrove and coral reef ecosystems." International Journal of Systematic and Evolutionary Microbiology 60, no. 8 (August 1, 2010): 1978–84. http://dx.doi.org/10.1099/ijs.0.016857-0.

Full text

Abstract:

Three species of yeasts are taxonomically described for strains isolated from marine environments. Candida spencermartinsiae sp. nov. (type strain CBS 10894T =NRRL Y-48663T) and Candida taylorii sp. nov. (type strain CBS 8508T =NRRL Y-27213T) are anamorphic ascomycetous yeasts in a phylogenetic cluster of marine yeasts in the Debaryomyces/Lodderomyces clade of the Saccharomycetales. The two species were isolated from multiple locations among coral reefs and mangrove habitats. Pseudozyma abaconensis sp. nov. (type strain CBS 8380T =NRRL Y-17380T) is an anamorphic basidiomycete that is related to the smut fungi of the genus Ustilago in the Ustilaginales. P. abaconensis was collected from waters adjacent to a coral reef.

APA, Harvard, Vancouver, ISO, and other styles

9

Shi, Cheng-Feng, Kai-Hong Zhang, Chun-Yue Chai, Zhen-Li Yan, and Feng-Li Hui. "Diversity of the genus Sugiyamaella and description of two new species from rotting wood in China." MycoKeys 77 (January 12, 2021): 27–39. http://dx.doi.org/10.3897/mycokeys.77.60077.

Full text

Abstract:

Species of the genus Sugiyamaella (Trichomonascaceae, Saccharomycetales), found in rotting wood in China, were investigated using morphology and the molecular phylogeny of a combined ITS and nrLSU dataset. Nine taxa were collected in China: two were new species (viz. Sugiyamaella chuxiongsp. nov. and S. yunanensissp. nov.) and seven were known species, S. americana, S. ayubii, S. novakii, S. paludigena, S. valenteae, S. valdiviana and S. xiaguanensis. The two new species are illustrated and their morphology and phylogenetic relationships with other Sugiyamaella species are discussed. Our results indicate a potentially great diversity of Sugiyamaella spp. inhabiting rotting wood in China just waiting to be discovered.

APA, Harvard, Vancouver, ISO, and other styles

10

P. Kurtzman, Cletus. "Trichomonascus petasosporus sp. nov. and Sympodiomyces indianaensis sp. nov., two new members of the Saccharomycetales." Antonie van Leeuwenhoek 85, no. 4 (May 2004): 297–304. http://dx.doi.org/10.1023/b:anto.0000020382.23285.db.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Wang, Long, Marizeth Groenewald, Qi-Ming Wang, and Teun Boekhout. "Reclassification of Saccharomycodes sinensis, Proposal of Yueomyces sinensis gen. nov., comb. nov. within Saccharomycetaceae (Saccharomycetales, Saccharomycotina)." PLOS ONE 10, no. 9 (September 16, 2015): e0136987. http://dx.doi.org/10.1371/journal.pone.0136987.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Le Thuong, Hoang Thi, Nguyen Quang Hao, and Tran Thi Thuy. "Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple." TAP CHI SINH HOC 39, no. 4 (February 5, 2018): 474–82. http://dx.doi.org/10.15625/0866-7160/v39n4.10864.

Full text

Abstract:

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8 belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: thuy_tt@hnue.edu.vn Received 5 December 2016, accepted 12 August 2017

APA, Harvard, Vancouver, ISO, and other styles

13

Jutakanoke, Rumpa, Rikiya Endoh, Masako Takashima, Moriya Ohkuma, Somboon Tanasupawat, and Ancharida Akaracharanya. "Allodekkera sacchari gen. nov., sp. nov., a yeast species in the Saccharomycetales isolated from a sugar factory." International Journal of Systematic and Evolutionary Microbiology 67, no. 2 (February 1, 2017): 250–55. http://dx.doi.org/10.1099/ijsem.0.001607.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Ren, Yong-Cheng, Yun Wang, Liang Chen, Tao Ke, and Feng-Li Hui. "Wickerhamiella allomyrinae f.a., sp. nov., a yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma." International Journal of Systematic and Evolutionary Microbiology 64, Pt_11 (November 1, 2014): 3856–61. http://dx.doi.org/10.1099/ijs.0.068403-0.

Full text

Abstract:

Two strains representing Wickerhamiella allomyrinae f.a., sp. nov. were isolated from the gut of Allomyrina dichotoma (Coleoptera: Scarabeidae) collected from the Baotianman National Nature Reserve, Nanyan, Henan Province, China. Sequence analyses of the D1/D2 domains of the LSU rRNA gene revealed that this novel species was located in the Wickerhamiella clade (Saccharomycetes, Saccharomycetales), with three described species of the genus Candida, namely Candida musiphila, Candida spandovensis and Candida sergipensis, as the most closely related species. The novel species differed from these three species by 9.3–9.8 % sequence divergence (35–45 nt substitutions) in the D1/D2 sequences. The species could also be distinguished from the closely related species, C. musiphila, C. spandovensis and C. sergipensis, by growth on vitamin-free medium and at 37 °C. The type strain is Wickerhamiella allomyrinae sp. nov. NYNU 13920T ( = CICC 33031T = CBS 13167T).

APA, Harvard, Vancouver, ISO, and other styles

15

Dayo-Owoyemi, Ifeloju, Carlos Augusto Rosa, André Rodrigues, and Fernando Carlos Pagnocca. "Wickerhamiella kiyanii f.a., sp. nov. and Wickerhamiella fructicola f.a., sp. nov., two yeasts isolated from native plants of Atlantic rainforest in Brazil." International Journal of Systematic and Evolutionary Microbiology 64, Pt_6 (June 1, 2014): 2152–58. http://dx.doi.org/10.1099/ijs.0.058784-0.

Full text

Abstract:

Two novel species, Wickerhamiella kiyanii f.a., sp. nov. (type strain FB1-1DASPT = CBS 12905T = CBMAI 1613T) and Wickerhamiella fructicola f.a., sp. nov. (type strain H10YT = CBS 12902T = CBMAI 1614T) are proposed in the Wickerhamiella clade (Saccharomycetes, Saccharomycetales) to accommodate three strains isolated from flowers and fruits typical of the Brazilian Atlantic rainforest. The novel status of these yeast species was established by sequence divergence observed in the D1/D2 domains of the LSU rRNA gene from the most closely related, described species as well as by phylogenetic analysis. Wickerhamiella kiyanii sp. nov. differs from its nearest phylogenetic neighbours W. pagnoccae CBS 12178T, Candida jalapaonensis CBS 10935T and Candida drosophilae CBS 8459T by 2.2–4.2 % in the D1/D2 sequences. By contrast, a sequence divergence of 13.2–13.8 % was observed between W. fructicola sp. nov. and its closest, described phylogenetic relative Candida kazoui JCM 12558T and Candida hasegawae JCM 12559T. Taxonomic descriptions of the two novel species are given.

APA, Harvard, Vancouver, ISO, and other styles

16

Khunnamwong, Pannida, Noppon Lertwattanasakul, Sasitorn Jindamorakot, Savitree Limtong, and Marc-André Lachance. "Description of Diutina gen. nov., Diutina siamensis, f.a. sp. nov., and reassignment of Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida ranongensis, Candida rugosa and Candida scorzettiae to the genus Diutina." International Journal of Systematic and Evolutionary Microbiology 65, Pt_12 (December 1, 2015): 4701–9. http://dx.doi.org/10.1099/ijsem.0.000634.

Full text

Abstract:

Three strains (DMKU-RE28, DMKU-RE43T and DMKU-RE123) of a novel anamorphic yeast species were isolated from rice leaf tissue collected in Thailand. DNA sequence analysis demonstrated that the species forms a sister pair with Candida ranongensis CBS 10861T but differs by 24–30 substitutions in the LSU rRNA gene D1/D2 domains and 30–35 substitutions in the ITS region. A phylogenetic analysis based on both the small and the large rRNA gene subunits confirmed this connection and demonstrated the presence of a clade that also includes Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida rugosa and Candida scorzettiae. The clade is not closely affiliated to any known teleomorphic genus, and forms a well-separated lineage from currently recognized genera of the Saccharomycetales. Hence, the genus Diutina gen. nov. is proposed to accommodate members of the clade, including Diutina siamensis f.a. sp. nov. and the preceding seven Candida species. The type strain is DMKU-RE43T ( = CBS 13388T = BCC 61183T = NBRC 109695T).

APA, Harvard, Vancouver, ISO, and other styles

17

Guarro, Josep, Josepa Gené, and Alberto M. Stchigel. "Developments in Fungal Taxonomy." Clinical Microbiology Reviews 12, no. 3 (July 1, 1999): 454–500. http://dx.doi.org/10.1128/cmr.12.3.454.

Full text

Abstract:

SUMMARY Fungal infections, especially those caused by opportunistic species, have become substantially more common in recent decades. Numerous species cause human infections, and several new human pathogens are discovered yearly. This situation has created an increasing interest in fungal taxonomy and has led to the development of new methods and approaches to fungal biosystematics which have promoted important practical advances in identification procedures. However, the significance of some data provided by the new approaches is still unclear, and results drawn from such studies may even increase nomenclatural confusion. Analyses of rRNA and rDNA sequences constitute an important complement of the morphological criteria needed to allow clinical fungi to be more easily identified and placed on a single phylogenetic tree. Most of the pathogenic fungi so far described belong to the kingdom Fungi; two belong to the kingdom Chromista. Within the Fungi, they are distributed in three phyla and in 15 orders (Pneumocystidales, Saccharomycetales, Dothideales, Sordariales, Onygenales, Eurotiales, Hypocreales, Ophiostomatales, Microascales, Tremellales, Poriales, Stereales, Agaricales, Schizophyllales, and Ustilaginales).

APA, Harvard, Vancouver, ISO, and other styles

18

Fang, Sheng, Zhening Jin, Yisong Xu, Ruyi Sha, Jianwei Mao, and Zengliang Jiang. "Chinese bayberry Jiaosu fermentation – changes of mycobiota composition and antioxidant properties." International Journal of Food Engineering 17, no. 6 (June 1, 2020): 455–63. http://dx.doi.org/10.1515/ijfe-2020-0238.

Full text

Abstract:

Abstract The aim of the study was to investigate the dynamic changes of mycobiota community, and the resultant effects on the antioxidant properties during the Chinese bayberry Jiaosu fermentation. The structure and composition of mycobiota during the Chinese bayberry Jiaosu fermentation were significantly changed (p < 0.001) and clearly clustered into two distinct phases (Phase 1: Day 5–20; Phase 2: Day 30–60, p < 0.001). From Phase 1 to Phase 2, the dominant fungi gradually changed from Saccharomycetales fam Incertae sedis to Saccharomyces cerevisiae. The antioxidative properties (total polyphenols, 1,1-diphenyl-2-picrylhydrazyl [DPPH], superoxide and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonate) [ABTS] radical scavenging) of Chinese bayberry Jiaosu were significantly increased by 250.4, 73.9, 25.3 and 40.0% respectively (p < 0.001). Co-occurrence network analysis revealed that Saccharomyces cerevisiae contributed to the increase of antioxidative properties in the Chinese bayberry Jiaosu fermentation. Our research indicates that fermentation into Chinese bayberry Jiaosu is an effective and new method for high-valued utilization of Chinese bayberry.

APA, Harvard, Vancouver, ISO, and other styles

19

Boontham, Wanatchaporn, Jirameth Angchuan, Chanita Boonmak, and Nantana Srisuk. "Limtongozyma siamensis gen. nov., sp. nov., a yeast species in the Saccharomycetales and reassignment of Candida cylindracea to the genus Limtongozyma." International Journal of Systematic and Evolutionary Microbiology 70, no. 1 (January 1, 2020): 199–203. http://dx.doi.org/10.1099/ijsem.0.003735.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Chikano, Mao, and Jun-ichi Takahashi. "Complete mitochondrial DNA sequence of the yeast Zygosaccharomyces siamensis (Saccharomycetes: Saccharomycetales) from fermented honey of the Apis cerana japonica in Japan." Mitochondrial DNA Part B 5, no. 3 (July 2, 2020): 2645–47. http://dx.doi.org/10.1080/23802359.2020.1785961.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Chen, Shan-Fu, Shih Feng Lo, Chin-Feng Chang, and Ching-Fu Lee. "Tetrapisispora taiwanensis sp. nov. and Tetrapisispora pingtungensis sp. nov., two ascosporogenous yeast species isolated from soil." International Journal of Systematic and Evolutionary Microbiology 63, Pt_6 (June 1, 2013): 2351–55. http://dx.doi.org/10.1099/ijs.0.051227-0.

Full text

Abstract:

Two novel yeast species, Tetrapisispora taiwanensis sp. nov. and Tetrapisispora pingtungensis sp. nov., belonging to the Saccharomycetaceae within the Saccharomycetales, are proposed to accommodate six strains isolated from samples of Taiwanese forest soil between 2005 and 2010. Sequence analysis of the internal transcribed spacer (ITS) and the D1/D2 domains of the large-subunit (LSU) rRNA gene revealed that the two species are phylogenetically closely related to species of the genus Tetrapisispora. Moreover, the assimilation spectrum of carbon and nitrogen sources and morphological characteristics are very similar to those of other Tetrapisispora species. The molecular, morphological and physiological characteristics described above indicate that these two species should be classified as members of the genus Tetrapisispora. The two species can be differentiated from each other and from other Tetrapisispora species based on their LSU D1/D2 rRNA gene and ITS sequences. Thus, the two species could be regarded as novel species of the genus Tetrapisispora, and the names Tetrapisispora taiwanensis sp. nov. (type strain SJ6S04T = CBS 10586T = NBRC 102652T = BCRC 23090T) and Tetrapisispora pingtungensis sp. nov. (type strain NC2S06T = CBS 12780T = BCRC 23409T) are proposed. The type strains of the two species were isolated from forest soil in Leinhuatsu Park, Nantou, in 2006 and from Hungchun, Pingtung, in 2009, respectively.

APA, Harvard, Vancouver, ISO, and other styles

22

Kurtzman, Cletus P., and Christie J. Robnett. "Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales." FEMS Yeast Research 10, no. 3 (April 12, 2010): 353–61. http://dx.doi.org/10.1111/j.1567-1364.2010.00625.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Nasim, Faiz-ul-Hassan, Adeela Naureen, Muhammad Saleem, Naheed Riaz, Muhammad Shafiq Choudhary, and Muhammad Ashraf. "PAAN135, a novel rhizospheric fungus associated with Cholistan desert grass Panicum antidotale, is a species of Saccharomycetales and a new source of cyclo-L-prolylglycine diketopiperazine." Symbiosis 74, no. 2 (June 23, 2017): 121–30. http://dx.doi.org/10.1007/s13199-017-0495-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Arup, Ulf, and Martin Grube. "Where does Lecanora Demissa (Ascomycota, Lecanorales) Belong?" Lichenologist 31, no. 5 (September 1999): 419–30. http://dx.doi.org/10.1006/lich.1999.0227.

Full text

Abstract:

AbstractLecanora demissa (Körb.) Zahlbr. is a crustose, lobate lichen that produces soredia and conidiomata but no apothecia. Its placement in Lecanora has long been questioned but nothing better has been proposed. We have studied the nuclear rDNA of the ITS regions and the SSU of L. demissa. In an alignment of the ITS regions of several representatives of Lecanora s. lat. it could clearly be shown by a PAUP analysis, using Aspicilia caesiocinerea as outgroup, that L. demissa does not belong to Lecanora. In a PAUP analysis of sequences of the SSU from representatives of the order Lecanorales, using members of Saccharomycetales as outgroup, L. demissa clustered on a well-supported branch with Caloplaca chlorina. In a further analysis of the ITS sequences of L. demissa together with representatives of Caloplaca and Xanthoria using Protoparmelia as outgroup two most parsimonious trees were found. In these trees the L. demissa branch was well within a strongly supported clade with C. cerina, the type species of the genus Caloplaca. The sister taxon to L. demissa in this analysis was C. variabilis. Chemical data and characters of the conidiomata support the affinity with Caloplaca and the new combination C. demissa (Körb.) Arup & Grube is therefore proposed. A lectotype for Imbricaria demissa has been designated. The phylogenetic analysis of several representatives of the genera Caloplaca and Xanthoria suggests that these genera are not monophyletic as presently circumscribed. Two large, monophyletic groups of species could be recognized, one with Xanthoria species mixed with lobate and crustose members of Caloplaca, and one with mainly crustose Caloplaca, including both species with orange or black apothecia.

APA, Harvard, Vancouver, ISO, and other styles

25

Lachance, M. A., and C. P. Kurtzman. "The yeast genus Tortispora gen. nov., description of Tortispora ganteri sp. nov., Tortispora mauiana f.a., sp. nov., Tortispora agaves f.a., sp. nov., Tortispora sangerardonensis f.a., sp. nov., Tortispora cuajiniquilana f.a., sp. nov., Tortispora starmeri f.a., sp. nov. and Tortispora phaffii f.a., sp. nov., reassignment of Candida caseinolytica to Tortispora caseinolytica f.a., comb. nov., emendation of Botryozyma, and assignment of Botryozyma, Tortispora gen. nov. and Trigonopsis to the family Trigonopsidaceae fam. nov." International Journal of Systematic and Evolutionary Microbiology 63, Pt_8 (August 1, 2013): 3104–14. http://dx.doi.org/10.1099/ijs.0.052209-0.

Full text

Abstract:

We describe the yeast genus Tortispora gen. nov., an early-diverging lineage in the Saccharomycetales that displays the formation of helical ascospores. The genus is based on 16 strains resembling Candida caseinolytica that were isolated from necrotic plant tissue in warm regions of the New World. Based on sequences of the D1/D2 domains of the nuclear large subunit rRNA gene, as well as other data, the strains are assigned to eight distinct species. The species are nutritionally specialized and share the unusual ability to hydrolyse casein and to grow on 1-butanol as sole carbon source. One species of the proposed new genus produces a simple ascus with a helical ascospore, whereas other species of the clade have failed to form ascospores. All species in the clade, including C. caseinolytica, are assigned to Tortispora gen. nov. The new binomials are Tortispora ganteri sp. nov., type species of the genus (SUB 86-469.5T = CBS 12581T = NRRL Y-17035T), Tortispora caseinolytica f.a., comb. nov. (UCD-FST 83-438.3T = CBS 7781T = NRRL Y-17796T), Tortispora mauiana f.a., sp. nov. (UWOPS 87-2430.3T = CBS 12803T = NRRL Y-48832T), Tortispora agaves f.a., sp. nov. (UWOPS 94-257.6T = CBS 12794T = NRRL Y-63662T), Tortispora sangerardonensis f.a., sp. nov. (UWOPS 00-157.1T = CBS 12795T = NRRL Y-63663T), Tortispora cuajiniquilana f.a., sp. nov. (UWOPS 99-344.4T = CBS 12796T = NRRL Y-63664T), Tortispora starmeri f.a., sp. nov. (G 91-702.5T = CBS 12793T = NRRL Y-63665T) and Tortispora phaffii f.a., sp. nov. (UWOPS 91-445.1T = CBS 12804T = NRRL Y-48833T). In addition, species formerly assigned to the genus Ascobotryozyma are reassigned to the genus Botryozyma. The genera Trigonopsis, Botryozyma and Tortispora are assigned to the family Trigonopsidaceae fam. nov.

APA, Harvard, Vancouver, ISO, and other styles

26

Luo, Qingwei, Lee R. Krumholz, Fares Z. Najar, Aaron D. Peacock, Bruce A. Roe, David C. White, and Mostafa S. Elshahed. "Diversity of the Microeukaryotic Community in Sulfide-Rich Zodletone Spring (Oklahoma)." Applied and Environmental Microbiology 71, no. 10 (October 2005): 6175–84. http://dx.doi.org/10.1128/aem.71.10.6175-6184.2005.

Full text

Abstract:

ABSTRACT The microeukaryotic community in Zodletone Spring, a predominantly anaerobic sulfide and sulfur-rich spring, was examined using an 18S rRNA gene cloning and sequencing approach. The majority of the 288 clones sequenced from three different locations at Zodletone Spring belonged to the Stramenopiles, Alveolata, and Fungi, with members of the phylum Cercozoa, order Diplomonadida, and family Jakobidae representing a minor fraction of the clone library. No sequences suggesting the presence of novel kingdom level diversity were detected in any of the three libraries. A large fraction of stramenopile clones encountered were monophyletic with either members of the genus Cafeteria (order Bicosoecida) or members of the order Labyrinthulida (slime nets), both of which have so far been encountered mainly in marine habitats. The majority of the observed fungal clone sequences belonged to the ascomycetous yeasts (order Saccharomycetales), were closely related to yeast genera within the Hymenobasidiomycetes (phylum Basidiomycetes), or formed a novel fungal lineage with several previously published or database-deposited clones. To determine whether the unexpected abundance of fungal sequences in Zodletone Spring clone libraries represents a general pattern in anaerobic habitats, we generated three clone libraries from three different anaerobic settings (anaerobic sewage digester, pond sediment, and hydrocarbon-exposed aquifer sediments) and partially sequenced 210 of these clones. Phylogenetic analysis indicated that clone sequences belonging to the kingdom Fungi represent a significant fraction of all three clone libraries, an observation confirmed by phospholipid fatty acid and ergosterol analysis. Overall, this work reveals an unexpected abundance of Fungi in anaerobic habitats, describes a novel, yet-uncultured group of Fungi that appears to be widespread in anaerobic habitats, and indicates that several of the previously considered marine protists could also occur in nonmarine habitats.

APA, Harvard, Vancouver, ISO, and other styles

27

Panaiotov, Stefan, Yordan Hodzhev, Borislava Tsafarova, Vladimir Tolchkov, and Reni Kalfin. "Culturable and Non-Culturable Blood Microbiota of Healthy Individuals." Microorganisms 9, no. 7 (July 8, 2021): 1464. http://dx.doi.org/10.3390/microorganisms9071464.

Full text

Abstract:

Next-generation sequencing (NGS) and metagenomics revolutionized our capacity for analysis and identification of the microbial communities in complex samples. The existence of a blood microbiome in healthy individuals has been confirmed by sequencing, but some researchers suspect that this is a cell-free circulating DNA in blood, while others have had isolated a limited number of bacterial and fungal species by culture. It is not clear what part of the blood microbiota could be resuscitated and cultured. Here, we quantitatively measured the culturable part of blood microbiota of healthy individuals by testing a medium supplemented with a high concentration of vitamin K (1 mg/mL) and culturing at 43 °C for 24 h. We applied targeted sequencing of 16S rDNA and internal transcribed spacer (ITS) markers on cultured and non-cultured blood samples from 28 healthy individuals. Dominant bacterial phyla among non-cultured samples were Proteobacteria 92.97%, Firmicutes 2.18%, Actinobacteria 1.74% and Planctomycetes 1.55%, while among cultured samples Proteobacteria were 47.83%, Firmicutes 25.85%, Actinobacteria 16.42%, Bacteroidetes 3.48%, Cyanobacteria 2.74%, and Fusobacteria 1.53%. Fungi phyla Basidiomycota, Ascomycota, and unidentified fungi were 65.08%, 17.72%, and 17.2% respectively among non-cultured samples, while among cultured samples they were 58.08%, 21.72%, and 20.2% respectively. In cultured and non-cultured samples we identified 241 OTUs belonging to 40 bacterial orders comprising 66 families and 105 genera. Fungal biodiversity accounted for 272 OTUs distributed in 61 orders, 105 families, and 133 genera. Bacterial orders that remained non-cultured, compared to blood microbiota isolated from fresh blood collection, were Sphingomonadales, Rhizobiales, and Rhodospirillales. Species of orders Bacillales, Lactobacillales, and Corynebacteriales showed the best cultivability. Fungi orders Tremellales, Polyporales, and Filobasidiales were mostly unculturable. Species of fungi orders Pleosporales, Saccharomycetales, and Helotiales were among the culturable ones. In this study, we quantified the capacity of a specific medium applied for culturing of blood microbiota in healthy individuals. Other culturing conditions and media should be tested for optimization and better characterization of blood microbiota in healthy and diseased individuals.

APA, Harvard, Vancouver, ISO, and other styles

28

García, Margarita, Braulio Esteve-Zarzoso, Juan Cabellos, and Teresa Arroyo. "Advances in the Study of Candida stellata." Fermentation 4, no. 3 (September 4, 2018): 74. http://dx.doi.org/10.3390/fermentation4030074.

Full text

Abstract:

Candida stellata is an imperfect yeast of the genus Candida that belongs to the order Saccharomycetales, while phylum Ascomycota. C. stellata was isolated originally from a must overripe in Germany but is widespread in natural and artificial habitats. C. stellata is a yeast with a taxonomic history characterized by numerous changes; it is either a heterogeneous species or easily confused with other yeast species that colonize the same substrates. The strain DBVPG 3827, frequently used to investigate the oenological properties of C. stellata, was recently renamed as Starmerella bombicola, which can be easily confused with C. zemplinina or related species like C. lactis-condensi. Strains of C. stellata have been used in the processing of foods and feeds for thousands of years. This species, which is commonly isolated from grape must, has been found to be competitive and persistent in fermentation in both white and red wine in various wine regions of the world and tolerates a concentration of at least 9% (v/v) ethanol. Although these yeasts can produce spoilage, several studies have been conducted to characterize C. stellata for their ability to produce desirable metabolites for wine flavor, such as acetate esters, or for the presence of enzymatic activities that enhance wine aroma, such as β-glucosidase. This microorganism could also possess many interesting technological properties that could be applied in food processing. Exo and endoglucosidases and polygalactosidase of C. stellata are important in the degradation of β-glucans produced by Botrytis cinerea. In traditional balsamic vinegar production, C. stellata shapes the aromatic profile of traditional vinegar, producing ethanol from fructose and high concentrations of glycerol, succinic acid, ethyl acetate, and acetoin. Chemical characterization of exocellular polysaccharides produced by non-Saccharomyces yeasts revealed them to essentially be mannoproteins with high mannose contents, ranging from 73–74% for Starmerella bombicola. Numerous studies have clearly proven that these macromolecules make multiple positive contributions to wine quality. Recent studies on C. stellata strains in wines made by co-fermentation with Saccharomyces cerevisiae have found that the aroma attributes of the individual strains were apparent when the inoculation protocol permitted the growth and activity of both yeasts. The exploitation of the diversity of biochemical and sensory properties of non-Saccharomyces yeast could be of interest for obtaining new products.

APA, Harvard, Vancouver, ISO, and other styles

29

Waldl, Maria, Bernhard Thiel, Roman Ochsenreiter, Alexander Holzenleiter, João de Araujo Oliveira, Maria Walter, Michael Wolfinger, and Peter Stadler. "TERribly Difficult: Searching for Telomerase RNAs in Saccharomycetes." Genes 9, no. 8 (July 26, 2018): 372. http://dx.doi.org/10.3390/genes9080372.

Full text

Abstract:

The telomerase RNA in yeasts is large, usually >1000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very difficult to detect by homology-based methods and so far have escaped annotation in the majority of the genomes of Saccharomycotina. This is a consequence of sequences that evolve rapidly at nucleotide level, are subject to large variations in size, and are highly plastic with respect to their secondary structures. Here, we report on a survey that was aimed at closing this gap in RNA annotation. Despite considerable efforts and the combination of a variety of different methods, it was only partially successful. While 27 new telomerase RNAs were identified, we had to restrict our efforts to the subgroup Saccharomycetacea because even this narrow subgroup was diverse enough to require different search models for different phylogenetic subgroups. More distant branches of the Saccharomycotina remain without annotated telomerase RNA.

APA, Harvard, Vancouver, ISO, and other styles

30

Van Timmeren, Steven, Philip D. Fanning, Torsten Schöneberg, Kelly Hamby, Jana Lee, and Rufus Isaacs. "Exploring the Efficacy and Mechanisms of a Crop Sterilant for Reducing Infestation by Spotted-Wing Drosophila (Diptera: Drosophilidae)." Journal of Economic Entomology, October 20, 2019. http://dx.doi.org/10.1093/jee/toz245.

Full text

Abstract:

Abstract Vinegar flies (Diptera: Drosophilidae) are well known to be associated with yeasts, which provide important nutrients and emit attractive semiochemicals. Drosophila suzukii (Matsumura) has become a major pest of berries and cherries around the world, requiring intensive management to maintain fruit quality. Although insecticides remain a dominant control approach, disruption of fly–yeast–host interactions remains a promising avenue for reducing the economic impact of this pest. We conducted field and laboratory experiments to explore whether a crop sterilant (peroxyacetic acid and hydrogen peroxide) developed for disease control can affect D. suzukii. In 2 yr of field tests in highbush blueberries, we found significantly lower infestation by D. suzukii in plots treated with the crop sterilant, both alone and in a rotation program with zeta-cypermethrin. When shoots from treated plots were tested in no-choice bioassays, crop sterilant treatments did not affect adult mortality or oviposition, but they reduced infestation. To explore the mechanisms in the laboratory, we found that the crop sterilant did not affect adult mortality, nor oviposition on treated fruit under no-choice settings, but adult flies settled and oviposited less on treated fruit in choice settings. When the crop sterilant was applied to colonies of Hanseniaspora uvarum (Niehaus) (Saccharomycetales: Saccharomycodaceae) and Issatchenkia terricola (Van der Walt) (Saccharomycetales: Saccharomycetacea) yeasts that are attractive and provide nutrition to D. suzukii, there was a dose-dependent inhibition of their growth. We highlight the potential for microbial management as a component of integrated pest management programs and prioritize research needs to incorporate this approach into control programs.

APA, Harvard, Vancouver, ISO, and other styles

31

Ortiz-Álvarez, Jossue, Arturo Becerra-Bracho, Alfonso Méndez-Tenorio, Jazmin Murcia-Garzón, Lourdes Villa-Tanaca, and César Hernández-Rodríguez. "Phylogeny, evolution, and potential ecological relationship of cytochrome CYP52 enzymes in Saccharomycetales yeasts." Scientific Reports 10, no. 1 (June 24, 2020). http://dx.doi.org/10.1038/s41598-020-67200-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Hesselbart, Ana, Klara Junker, and Jürgen Wendland. "Draft Genome Sequence of Saccharomycopsis fermentans CBS 7830, a Predacious Yeast Belonging to the Saccharomycetales." Genome Announcements 6, no. 2 (January 11, 2018). http://dx.doi.org/10.1128/genomea.01445-17.

Full text

Abstract:

ABSTRACT Saccharomycopsis fermentans is an ascomycetous necrotrophic fungal pathogen that penetrates and kills fungal prey cells via targeted penetration pegs. Here, we report the draft genome sequence and scaffold assembly of this mycoparasite.

APA, Harvard, Vancouver, ISO, and other styles

33

Rahmat, Endang, Inkyu Park, and Youngmin Kang. "The whole-genome sequence of the novel yeast species Metschnikowia persimmonesis isolated from medicinal plant Diospyros kaki Thunb." G3 Genes|Genomes|Genetics, July 13, 2021. http://dx.doi.org/10.1093/g3journal/jkab246.

Full text

Abstract:

Abstract The new yeast Metschnikowia persimmonesis KCTC 12991BP (KIOM G15050 strain) exhibits strong antimicrobial activity against some pathogens. This activity may be related to the medicinal profile of secondary metabolites that could be found in the genome of this species. Therefore, to explore its future possibility of producing some beneficial activities, including medicinal ability, we report high-quality whole-genome assembly of M. persimmonesis produced by PacBio RS II sequencer. The final draft assembly consisted of 16 scaffolds with GC content of 45.90% and comprised a fairly complete set (82.8%) of BUSCO result using Saccharomycetales lineage data set. The total length of the genome was 16.473 Mb, with a scaffold N50 of 1.982 Mb. Annotation of the M. persimmonesis genome revealed presence of 7029 genes and 6939 functionally annotated proteins. Based on the analysis of phylogenetic relationship and the average nucleotide identities, M. persimmonesis was proved to a novel species within the Metschnikowia genus. This finding is expected to significantly contribute to the discovery of high-value natural products from M. persimmonesis as well as for genome biology and evolution comparative analysis within Metschnikowia species.

APA, Harvard, Vancouver, ISO, and other styles

34

Sun, Xiang Dong, Hong Shan, Lili Li, Ping Su, Jing Lan, Lin Zhao, and Huan Chun Yang. "Field Fungal Diversity in Freshly Harvested Maize." International Journal of Biochemistry Research & Review, June 11, 2019, 1–13. http://dx.doi.org/10.9734/ijbcrr/2019/v26i130088.

Full text

Abstract:

Maize is a major crop in China and maize production in Heilongjiang Province ranks No.1 in the country in annual maize production in the whole country. Maize is prone to invasion by fungi and mycotoxins produced by these fungi are proven to be serious threats to animals as well as human health. Through high through-put sequencing we detected the dominant phylum to be Ascomycota; Dothideomycetes, Sordariomycetes, Eurotiomycetes and Tremellomycetes, Saccharomycetes were the dominant classes; Hypocreales, Eurotiales, Capnodiales, Saccharomycetales, Tremellales, and Pleosporales were the main orders; Nectriaceae, Trichocomaceae, Cladosporiaceae, Debaryomycetaceae, Tremellaceae, and Pleosporaceae were major families; Gibberella, Cladosporium, Papiliotrema, Penicillium, Scheffersomyces, Talaromyces, and Epicoccum were the most abundant phylotypes at the genus level. Epicoccum_nigrum, Gibberella_zeae, Papiliotrema_flavescens, and Scheffersomyces_shehatae were the dominant fungal species. Great fungal diversity was observed in the maize samples harvested in the five major maize-growing regions in Heilongjiang Province. Maize-1 in Nenjiang County was observed to have the greatest fungal diversity and abundance among the five regions. Since some of the fungal species are mycotoxin producing, it is necessary to take precautions to ensure the maize is stored under safe conditions to prevent the occurrence of mycotoxins and the growth and reproduction of other fungi which results in deterioration in the quality of maize.

APA, Harvard, Vancouver, ISO, and other styles

35

višňovská, Denisa, Petr Pyszko, Martin Šigut, Martin Kostovčík, Miroslav Kolařík, Nela Kotásková, and Pavel Drozd. "Caterpillar gut and host plant phylloplane mycobiomes differ: a new perspective on fungal involvement in insect guts." FEMS Microbiology Ecology 96, no. 9 (June 10, 2020). http://dx.doi.org/10.1093/femsec/fiaa116.

Full text

Abstract:

ABSTRACT Compared with the highly diverse microbiota of leaves, herbivorous insects exhibit impoverished gut microbial communities. Research to date has focused on the bacterial component of these gut microbiomes, neglecting the fungal component. As caterpillar gut bacterial microbiomes are derived strongly from their diet, we hypothesized that their mycobiomes would reflect the host leaf mycobiomes. Using the ITS2 rDNA and V5–V6 16S rRNA gene regions for DNA metabarcoding of caterpillar gut and host leaf sample pairs we compared their mycobiome genus diversity and compositions and identified genera associated with caterpillar guts. Leaves and caterpillar guts harbored different mycobiomes with quite low qualitative similarity (Jaccard index = 38.03%). The fungal genera most significantly associated with the caterpillar gut included Penicillium, Mucor and unidentified Saccharomycetales, whereas leaf-associated genera included Holtermanniella, Gibberella (teleomorph of Fusarium) and Seimatosporium. Although caterpillar gut and leaf mycobiomes had similar genus richness overall, this indicator was not correlated for individual duplets. Moreover, as more samples entered the analysis, mycobiome richness increased more rapidly in caterpillar guts than in leaves. The results suggest that the mycobiota of the caterpillar gut differs from that of their feeding substrate; further, the mycobiomes appear to be richer than the well-studied bacterial microbiotas.

APA, Harvard, Vancouver, ISO, and other styles

36

Chen, Yanru, Kaimin Li, Ting Liu, Ruyi Li, Guiming Fu, Yin Wan, and Fuping Zheng. "Analysis of Difference in Microbial Community and Physicochemical Indices Between Surface and Central Parts of Chinese Special-Flavor Baijiu Daqu." Frontiers in Microbiology 11 (January 14, 2021). http://dx.doi.org/10.3389/fmicb.2020.592421.

Full text

Abstract:

Special-flavor Baijiu is a unique Baijiu in Jiangxi Province, China, whose uniqueness mainly depends on the unique production process of special-flavor Baijiu Daqu. However, the microbial structure and physicochemical indices of different parts of the special-flavor Baijiu Daqu are still unknown. This greatly reduces the actual value of Daqu in the production of special-flavor Baijiu. Therefore, culture-dependent and Illumina MiSeq sequencing methods were used to analyze the microbial structure of special-flavor Baijiu Daqu. The results indicated that there was a complicated microbial diversity in Chinese special-flavor Baijiu Daqu. The predominant bacterial communities were Bacillales, Lactobacillales, and Rhodospirillales, while Saccharomycetales and Eurotiales were the predominant fungal communities. Significant differences in microbial community and distribution were shown between the surface and central parts of Daqu. Acetobacter and Pichia genera were the predominant microorganisms in the surface part of Daqu, whereas Aspergillus, Kroppenstedtia, Oceanobacillus, and Bacillus genera were the predominant microorganisms in the central part of Daqu. Meantime, the different microbial distributions between the surface and central parts of Daqu caused the significant differences in the physicochemical indices. These results can provide an important theoretical basis for improving the brewing process and the quality of special-flavor Baijiu.

APA, Harvard, Vancouver, ISO, and other styles

37

Barbosa, Marcílio Souza, Suzanne Nunes Barbosa, Nilson Satoru Nagamoto, Luiz Carlos Forti, and Cícero lmeida. "Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits." Ciência Rural 48, no. 5 (May 21, 2018). http://dx.doi.org/10.1590/0103-8478cr20170353.

Full text

Abstract:

ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with the universal primers (ITS4 and ITS5), to amplify the ITS region. Sequencing was performed using the Sanger method. Sequences were subjected to BLAST, allowing the identification of nine different species of the orders Agaricales, Eurotiales, Hypocreales, Pleosporales, Saccharomycetales and Tremellales showing a variation in identity of 96-100%. Using “The Automatic Barcode Gap Discovery” analysis, nine groups were identified, corresponding to species described in NCBI. The K2P distances were used to generate a tree using Neighbour-joining, demonstrating that the species were grouped according to phylogenetic groups. We concluded that leaf-cutter ant colonies exhibited a wide variety of fungi and this study suggested that there is no correlation between the species of fungi isolated with the control method used on the ant nest.

APA, Harvard, Vancouver, ISO, and other styles

38

Huang, Juan, and Larry J. Gut. "Impact of Background Fruit Odors on Attraction of Drosophila suzukii (Diptera: Drosophilidae) to Its Symbiotic Yeast." Journal of Insect Science 21, no. 2 (March 1, 2021). http://dx.doi.org/10.1093/jisesa/ieab016.

Full text

Abstract:

Abstract Background odors produced by plants in the environment can interfere with the response of insects to a point-releasing attractant, especially when their compositions overlap. In this study, a series of binary choice tests was conducted in a wind tunnel to investigate whether background odors emitted from cherry, blueberry, blackberry, or raspberry fruits would affect the level of Drosophila suzukii (Matsumura) attraction to its symbiotic yeast, Hanseniaspora uvarum (Niehaus) (Saccharomycetales: Saccharomycetaceae). Whether an increase in the intensity of background odors would affect the attractiveness of H. uvarum to D. suzukii was also investigated, either by increasing the number of cherry or raspberry fruit per cup or by increasing the number of fruit cups surrounding the cup baited with the yeast. In wind tunnel assays, background fruit odors interfering with D. suzukii attraction to the yeast varied among fruit types. Raspberry odor inhibited the attractiveness of H. uvarum to the fly the most, followed by blackberry odor, whereas cherry and blueberry odors had no significant impact on the attraction. An increase in the intensity of odors by adding more cherry or raspberry fruit per cup did not increase the impact of fruit odor on the attraction; however, adding more raspberry cups around H. uvarum linearly decreased its attractiveness, suggesting that background host fruit abundance and likely increase in host odor may influence D. suzukii attraction to yeast odor depending on host species.

APA, Harvard, Vancouver, ISO, and other styles

39

Oslan, Siti Nurbaya, Abu Bakar Salleh, Raja Noor Zaliha Raja Abd Rahman, Mahiran Basri, and Adam Leow Thean Chor. "Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization." Acta Biochimica Polonica 59, no. 2 (May 11, 2012). http://dx.doi.org/10.18388/abp.2012_2142.

Full text

Abstract:

Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) Internal Transcribed Spacer (ITS). Full sequence of the rDNA ITS region for each isolate was BLASTed and phylogenetic study was constructed by using MEGA4. Among the isolates, isolate WB from 'ragi' (used to ferment carbohydrates) could be identified as a new species in order Saccharomycetales according to rDNA ITS region, morphology and biochemical tests. Isolate SO (from spoiled orange), RT (rotten tomato) and RG (different type of 'ragi') were identified as Pichia sp. Isolates R1 and R2, S4 and S5 (from the surrounding of a guava tree) were identified as Issatchenkia sp. and Hanseniaspora sp., respectively. Geneticin, 50 µg/mL, was determined to be the antibiotic marker for all isolates excepted for isolates RT and SO which used 500 µg/mL and 100 µg/mL Zeocin, respectively. Intra-extracellular proteins were screened for lipolytic activity at 30°C and 70°C. Thermostable lipase activity was detected in isolates RT and R1 with 0.6 U/mg and 0.1 U/mg, respectively. In conclusion, a new yeast-vector system for isolate WB can be developed by using phleomycin or geneticin as the drugs resistance marker. Moreover, strains RT and R1 can be investigated as a novel source of a thermostable lipase.

APA, Harvard, Vancouver, ISO, and other styles

40

Lehenberger, Maximilian, Nina Foh, Axel Göttlein, Diana Six, and Peter H. W. Biedermann. "Nutrient-Poor Breeding Substrates of Ambrosia Beetles Are Enriched With Biologically Important Elements." Frontiers in Microbiology 12 (April 26, 2021). http://dx.doi.org/10.3389/fmicb.2021.664542.

Full text

Abstract:

Fungus-farming within galleries in the xylem of trees has evolved independently in at least twelve lineages of weevils (Curculionidae: Scolytinae, Platypodinae) and one lineage of ship-timber beetles (Lymexylidae). Jointly these are termed ambrosia beetles because they actively cultivate nutritional “ambrosia fungi” as their main source of food. The beetles are obligately dependent on their ambrosia fungi as they provide them a broad range of essential nutrients ensuring their survival in an extremely nutrient-poor environment. While xylem is rich in carbon (C) and hydrogen (H), various elements essential for fungal and beetle growth, such as nitrogen (N), phosphorus (P), sulfur (S), potassium (K), calcium (Ca), magnesium (Mg), and manganese (Mn) are extremely low in concentration. Currently it remains untested how both ambrosia beetles and their fungi meet their nutritional requirements in this habitat. Here, we aimed to determine for the first time if galleries of ambrosia beetles are generally enriched with elements that are rare in uncolonized xylem tissue and whether these nutrients are translocated to the galleries from the xylem by the fungal associates. To do so, we examined natural galleries of three ambrosia beetle species from three independently evolved farming lineages, Xyleborinus saxesenii (Scolytinae: Xyleborini), Trypodendron lineatum (Scolytinae: Xyloterini) and Elateroides dermestoides (Lymexylidae), that cultivate unrelated ambrosia fungi in the ascomycete orders Ophiostomatales, Microascales, and Saccharomycetales, respectively. Several elements, in particular Ca, N, P, K, Mg, Mn, and S, were present in high concentrations within the beetles’ galleries but available in only very low concentrations in the surrounding xylem. The concentration of elements was generally highest with X. saxesenii, followed by T. lineatum and E. dermestoides, which positively correlates with the degree of sociality and productivity of brood per gallery. We propose that the ambrosia fungal mutualists are translocating essential elements through their hyphae from the xylem to fruiting structures they form on gallery walls. Moreover, the extremely strong enrichment observed suggests recycling of these elements from the feces of the insects, where bacteria and yeasts might play a role.

APA, Harvard, Vancouver, ISO, and other styles

41

Merwe, Marie van der, Sunita Sharma, Jade Caldwell, Nicholas Smith, Richard Bloomer, Randall Buddington, Charles Gomes, and Joseph Pierre. "Timed Feeding Protocols While Consuming a High Fat Diet Do Not Alter Gut Fungal Populations (OR23-06-19)." Current Developments in Nutrition 3, Supplement_1 (June 1, 2019). http://dx.doi.org/10.1093/cdn/nzz040.or23-06-19.

Full text

Abstract:

Abstract Objectives The gut microbiome participates in host metabolic regulation. While the vast majority of microbiome research has focused on bacterial populations, other microorganisms also colonize the mammalian intestine and likely play functionally important roles in host metabolism. The objective of current study was to characterize the role of dietary composition and timing strategies upon gut fungal populations. Methods C57BL/6 male mice were randomized to a Chow diet or a high-fat diet (HFD) for 6 weeks, followed by a switch from HFD to 1) Chow (sChow), 2) Purified High Fiber – Daniel Fast (DF), 3) HFD ad lib, 4) HFD time restricted (TRF), 5) HFD alternative day fasting (ADF), or 6) HFD 60% caloric restriction (CR) for an additional 8 weeks. Ileal, cecal and serial fecal samples were collected for next generation sequencing of ITS2 rRNA to examine the gut mycobiome. Results We observed dramatic reductions in alpha diversity in fecal fungal populations when animals consumed the HFD compared with Chow. HFD resulted in dramatic reduction in the relative abundance of the fungal order Saccharomyces, with a concomitant increases in the genus Candida and Hanseniaspora. In response to dietary switch from HFD to Chow, fungal taxonomic composition, alpha, and beta diversity transitioned to a population clustering more similarity with Chow by weeks 4 and 8 of intervention. After 8 weeks on the respective dietary interventions, alpha diversity of the ileal, cecal and fecal fungal population in mice consuming DF or various HFD fasting protocols remained similar to the HFD controls. Saccharomycetales remained the dominant genus present in HFD and DF groups. However, amongst these groups, the DF group (fecal sample) showed the greatest increase in Saccharomyces, but time-restricted feeding protocols also showed increased levels of Saccharomyces. Conclusions While fasting protocols on HFD are associated with improved metabolic outcomes, these data demonstrate that – similar to microbial populations within the microbiome – diet remains the largest driver of microorganism community composition. To our knowledge, this is the first investigation into the role of dietary timing strategies upon the gut fungal communities ever reported. Funding Sources University of Memphis. Children's Foundation Research Institute, Memphis.

APA, Harvard, Vancouver, ISO, and other styles

42

Bai, Chunsheng, Chao Wang, Lin Sun, Haiwen Xu, Yun Jiang, Na Na, Guomei Yin, Sibo Liu, and Yanlin Xue. "Dynamics of Bacterial and Fungal Communities and Metabolites During Aerobic Exposure in Whole-Plant Corn Silages With Two Different Moisture Levels." Frontiers in Microbiology 12 (June 15, 2021). http://dx.doi.org/10.3389/fmicb.2021.663895.

Full text

Abstract:

The study was aimed to investigate the effect of moisture content on microbial communities, metabolites, fermentation quality, and aerobic stability during aerobic exposure in whole-plant corn silages preserved long time to improve the quality and aerobic stability of the silage during feed-out. Corn plants with two different moisture levels (high-moisture content, 680 g/kg; low-moisture content, 620 g/kg) were harvested at one-third and two-thirds milk-line stages, respectively, ensiled in laboratory-scale silos, and then sampled at 350 day after ensiling and at 2 and 5 day after opening to investigate bacterial and fungal communities, metabolites, and aerobic stability. High-moisture content increased aerobic stability and pH and decreased lactic acid and microbial counts in silages (P < 0.05). During aerobic exposure, the low-moisture silages had higher pH and lactic acid bacterial count and lower lactic acid than the high-moisture silages (P < 0.05); Acinetobacter sp. was the most main bacterial species in the silages; Candida glabrata and unclassified Candida had an increasing abundance and negatively correlation with aerobic stability of high-moisture silages (P < 0.05), while C. glabrata, Candida xylopsoci, unclassified Saccharomycetaceae, and unclassified Saccharomycetales negative correlated with aerobic stability of low-moisture silages (P < 0.05) with a rising Saccharomycetaceae; the silages had a reducing concentration of total metabolites (P < 0.05). Moreover, the high-moisture silages contained greater total metabolites, saturated fatty acids (palmitic and stearic acid), essential fatty acids (linoleic acid), essential amino acids (phenylalanine), and non-essential amino acids (alanine, beta-alanine, and asparagine) than the low-moisture silages at 5 day of opening (P < 0.05). Thus, the high-moisture content improved the aerobic stability. Acinetobacter sp. and Candida sp. dominated the bacterial and fungal communities, respectively; Candida sp. resulted in the aerobic deterioration in high-moisture silages, while the combined activities of Candida sp. and Saccharomycetaceae sp. caused the aerobic deterioration in low-moisture silages. The greater aerobic stability contributed to preserve the palmitic acid, stearic acid, linoleic acid, phenylalanine, alanine, beta-alanine, and asparagine during aerobic exposure.

APA, Harvard, Vancouver, ISO, and other styles

43

Romero, J. J., J. Park, Y. Joo, Y. Zhao, M. Killerby, D. C. Reyes, F. Tiezzi, E. Gutierrez-Rodriguez, and M. S. Castillo. "A combination of Lactobacillus buchneri and Pediococcus pentosaceus extended the aerobic stability of conventional and BMR-corn hybrids ensiled at low dry matter concentrations by causing a major shift in their bacterial and fungal community." Journal of Animal Science, May 7, 2021. http://dx.doi.org/10.1093/jas/skab141.

Full text

Abstract:

Abstract We evaluated the effects of applying a combination inoculant to 4 corn hybrids harvested at high moisture on their nutritive value and microbial populations. The treatment design was the factorial combination of corn hybrids ensiled with (INO) and without (CON) inoculant. The hybrids were TMF2R737 (MCN), F2F817 (MBR), P2089YHR (PCN), and PI144XR (PBR), ensiled at dry matter (DM) concentrations of 30.5, 26.3, 31.1, and 31.5%, respectively; MBR and PBR were brown midrib mutants (BMR). The inoculant contained Lactobacillus buchneri and Pediococcus pentosaceus (4 × 10 5 and 1 × 10 5 cfu/g of fresh corn). The experiment had a complete randomized design with treatments replicated 6 times. Corn was treated or not with inoculant, packed into 7.6L bucket silos, and stored for 100 d. At d 0, the relative abundance (RA, %) of Enterobacteriaceae was lower in PBR vs. the other hybrids [51.3 vs x= (average of) 58.4] and in the case of fungi, incertae sedis (i.s.) Tremellales and Mucoraceae were more and less abundant, respectively, in conventional hybrids vs. BMRs (x= 25.8 vs. x= 13.9 and x= 3.64 vs. x= 7.52; P < 0.04). After ensiling, INO had higher LAB (9.3 vs. 7.1 log cfu/g of fresh corn) and acetic acid (3.44 vs. 1.32% of DM) and lower yeast (3.1 vs. 4.6) and molds (1.5 vs. 3.0), and also extended the aerobic stability (582 vs. 111h) but decreased DM recovery (95.6 vs. 97.4%) vs. CON (P < 0.02). Inoculation reduced bacterial phylogenetic diversity (6.75 vs. 14.4) but increased fungal observed taxonomical units (46 vs. 20) vs. CON (P < 0.01). Also, a higher relative abundance (RA) for Lactobacillaceae (99.2 vs. 75.7%) and lower for Enterobacteriaceae (0.28 vs. 9.93) was observed due to inoculation (P < 0.001). For fungi, INO had a lower RA compared to CON for Monascaceae (12.6 vs. 44.7) and increased i.s. Tremellales (8.0 vs. 1.2) and i.s. Saccharomycetales (6.4 vs. 0.3%; P < 0.006). Inoculation changed the diverse bacterial community found in the phyllosphere across hybrids to a taxonomically uneven one dominated by Lactobacillaceae. In the case of fungi, INO application increased the fungal diversity at d 100 mainly by reducing the dominance of Monascaceae vs. CON. In conclusion, the INO treatment overwhelmed the disparate microbial populations found across BMR and conventional hybrids ensiled at low DM concentrations and ensured a significant concentration of acetic acid that modified fungal populations and in turn extended the aerobic stability of all hybrids.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Saccharomycetales'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles