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Journal articles on the topic 'Salmonella paratyphi a biochemical reactions'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Zahra, Fatima Tuz, Sidrah Saleem, Muhammad Imran, Nida Javed, Ayesha ,. Ghazal, and Saadia Ch. "Association of Drinking Water and Enteric Fever: A Disguised Source of Infection." Pakistan Journal of Medical and Health Sciences 16, no. 5 (2022): 41–44. http://dx.doi.org/10.53350/pjmhs2216541.

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Background: Enteric fever is an illness caused by Salmonella enterica serovars Typhi and Paratyphi A and B. The mode of transmission is the ingestion of contaminated food and water. Aim: To check that whether drinking water is the source of infection or not. Methodology: 202 water samples were collected from the various areas of Lahore. 120 samples were taken from houses and the neighbourhood of confirmed typhoid patients and 82 samples were randomly collected. The samples were centrifuged, enriched and then subcultured on XLD media. The isolated colonies were identified by biochemical reactio

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Sudhaharan, Sukanya, Kanne Padmaja, Rachana Solanki, Vemu Lakshmi, Pamidi Umabala, and Bhaskara Aparna. "Extra-intestinal salmonellosis in a tertiary care center in South India." Journal of Infection in Developing Countries 8, no. 07 (2014): 831–37. http://dx.doi.org/10.3855/jidc.3731.

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Introduction: The present study is a retrospective analysis of a total of 36 cases of bacteriologically proven extra-intestinal salmonellosis, managed at Nizam’s Institute of Medical Sciences, between 1987 and 2012 (25 years). The extra-intestinal sites involved were the skin, cerebrum, spleen, ovary, synovium, and the skeletal muscle. Methodology: The extra-intestinal specimens were first processed using standard methods. Colonies suspected as Salmonella were identified by standard laboratory methods, initially by manual biochemical reactions and later by the API system (bioMerieux, Marcy l’E

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3

Eleazar, Clara Idara, Attama Daniella Chinemerem, and Rita Ngozi Nweke. "Antibiotic Susceptibility Patterns of Enteric Fever Salmonella Isolates from Infected Patients Blood Samples within Enugu, Nigeria." International Journal of Pathogen Research 13, no. 4 (2024): 64–71. http://dx.doi.org/10.9734/ijpr/2024/v13i4299.

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Background: Salmonella enterica enterica serovar typhi/paratyphi is the causative agent of enteric (typhoid) fever, a life-threatening systemic illness, significantly impacting global health especially the developing nations. Although enteric fever can be treated effectively with antibiotics, it is becoming increasingly challenging due to the rising incidence of drug resistance. Aim: The aim was investigate the prevalence and antibiotic susceptibility patterns of Salmonella isolates from typhoid fever patients in order identify the multidrug-resistant trends. Methodology: The study was carried

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Abdullahi, B., Abdulkadir, B., and Olayinka, B. O. "Occurrence of Salmonella serovars implicated in Salmonellosis in North-western part of Nigeria." UMYU Journal of Microbiology Research (UJMR) 2, no. 2 (2017): 141–52. http://dx.doi.org/10.47430/ujmr.1722.022.

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Accurate diagnosis of clinical Salmonella enterica and differentiation into its serovars is important from a public health and epidemiological point of view. Standard cultural, biochemical and polymerase chain reaction techniques were employed to isolate and identify Salmonella enteric serovars implicated in salmonellosis. This study was carried out between June 2015 and May 2016 in some selected hospitals in North-Western Nigeria. Four hundred and twenty stool samples were collected from patients clinically diagnosed of pyrexia and/or gastroenteritis. Of the 420 samples collected, 27 were pos

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Alam, J., T. Chakma, MS Islam, et al. "Pathology of Fowl Paratyphoid and Molecular Detection of Its Pathogen in Layer Chickens." Annals of Bangladesh Agriculture 24, no. 2 (2021): 47–57. http://dx.doi.org/10.3329/aba.v24i2.55783.

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The study was aimed to ascertain the pathology of fowl paratyphoid and molecular detection of its causal agent (Salmonella spp) in chickens. Pathological and swab samples were collected from layers in Gazipur district, Bangladesh. For observing the gross and microscopic lesions of different organs necropsy and histopathology were done, and to isolate and identify the Salmonella spp, different bacteriological tests and Polymerase Chain Reaction (PCR) were performed. Swabs from 150 chickens showed 66% of salmonellosis. Gram’s staining of isolated bacteria showed pink colored rod shaped bacilli.

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Alex Morel Longue Essoua, Fabrice Fabien Dongho Dongmo, Martin Luther Mogtomo Koanga, et al. "Study of coinfection of Salmonella typhi, Salmonella paratyphi with Candida spp., in four hospitals of Douala- Cameroon." International Journal of Science and Research Archive 2, no. 2 (2021): 110–19. http://dx.doi.org/10.30574/ijsra.2021.2.2.0041.

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Candida spp., are saprophytic yeasts capable of creating a favorable environment for bacterial infection, amplifying or inhibiting their activity. On the other hand, bacteria produce molecules that reduce the proliferation of Candida spp or promote their adherence to surfaces. The resurgence of typhoid and paratyphoid fevers in endemic individuals and the relative effectiveness of contemporary conventional therapies arouse curiosity to explore other factors that may influence, the re-emergence of infection in patients. We carried out a cross-sectional study of 2500 patients in four hospitals i

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Kim, Gyu Ri, Si Hyun Kim, Eun-Young Kim, et al. "Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species." Microorganisms 10, no. 10 (2022): 1974. http://dx.doi.org/10.3390/microorganisms10101974.

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Salmonella is a major pathogen causing foodborne infections in humans. Salmonella isolates are identified using biochemical and serological tests, including automated systems such as the VITEK2 system. However, there are few reports on Salmonella identification using VITEK MS. Therefore, we aimed to evaluate the usefulness of MALDI-TOF VITEK MS for Salmonella identification. A total of 1389 Salmonella isolates were identified using VITEK MS ver3.0 or ver3.2. All Salmonella isolates were confirmed by serotyping using the Kauffmann-White scheme, and the results were compared with the VITEK MS re

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Suman, Maiz-ul Ahad, Md Abdullah Siddique, S. M. Shamsuzzaman, et al. "Detection Of Mutated gyrA Gene From Nalidixic Acid Resistant Salmonella Typhi And Paratyphi A Isolated From Enteric Fever Patients In A Tertiary Care Hospital Of Bangladesh." Bangladesh Journal of Medical Microbiology 10, no. 1 (2017): 3–7. http://dx.doi.org/10.3329/bjmm.v10i1.31431.

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A cross sectional study for the detection of empirically used antibiotic resistant Salmonella was carried out in the department of microbiology of Rajshahi Medical College (RMC) and PCR and DNA sequencing were done in the department of microbiology of Dhaka Medical College during the period from August 2014 to July 2015.Total 323 blood samples were collected from suspected enteric fever patients from medicine and paediatric units of RMC hospital and cultured on brain heart infusion broth for isolation of Salmonella. Identification of Salmonella was done by biochemical tests and final identific

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9

Admassu, Dawit, Gudina Egata, and Zelalem Teklemariam. "Prevalence and antimicrobial susceptibility pattern of Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi among febrile patients at Karamara Hospital, Jigjiga, eastern Ethiopia." SAGE Open Medicine 7 (January 2019): 205031211983785. http://dx.doi.org/10.1177/2050312119837854.

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Objective: The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi among febrile patients at Karamara Hospital, Jigjiga, eastern Ethiopia. Methods: A cross-sectional study was conducted among 203 febrile patients presumptive of enteric fever ( Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi) at Karamara Hospital from 15 February to 20 March 2016. Venous blood was collected, cultured, and biochemical tests were performed. Antimicrobial susceptibility

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10

Bakhrouf, Amina, Moncef Jeddi, and Michel J. Gauthier. "Modification des caractères culturaux et biochimiques du Salmonella paratyphi B après incubation dans l'eau de mer." Canadian Journal of Microbiology 38, no. 8 (1992): 871–74. http://dx.doi.org/10.1139/m92-142.

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After incubation in seawater Salmonella paratyphi B cells rapidly became unable to grow on bacteriological media. Previous adaptation to high osmolarity conditions greatly slowed down this process. Strains isolated from seawater microcosms after varying incubation periods were qualitatively different and showed changes in some of their growth (colony shape and size) and biochemical properties (acidification of some sugars, gelatinase activity, acetoin production, nitrate reduction). Because of these modifications, the bacteria showed atypical profiles and could not be identified as members of

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Omotola, Justina. "Antibiotic Resistance of Typhoidal Salmonella Isolated from Humans in Kaduna and its Associated Factors." Open Access Journal of Microbiology & Biotechnology 6, no. 1 (2021): 1–7. http://dx.doi.org/10.23880/oajmb-16000181.

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Background: Pathogenic Salmonella species cause various types of infections ranging from mild gastroenteritis to life- threatening typhoid fever caused by Salmonella typhi , or paratyphoid fever caused by S. paratyphi A, B or C; of which S . paratyphi A is prevalent. Currently, treatment of typhoid infection is being hampered by the emergence of multidrug-resistant strains, while vaccines provide only temporal or partial protection. Objective: The aim of the study was to determine antibiotic resistance of typhoid causing Salmonella isolated from human stool. Methodology: A cross-sectional desc

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Hasan, Thamer O., Inam J Lafta, Emad A Ahmed, and Samah A Jassam. "Application of RAPD-PCR and Phylogenetic Analysis for Accurate ‎Characterization of ‎Salmonella‎‎‎ spp. Isolated from Chicken and Their Feed ‎and Drinking Water in Comparison ‎with API 20E, Vitek 2, and Serotyping." Iraqi Journal of Veterinary Medicine 47, no. 1 (2023): 11–20. http://dx.doi.org/10.30539/ijvm.v47i1.1493.

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    The aim of this study was ‎the‎ discrimination of Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, ‎including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, ‎and serology were used for bacterial identification. Random amplified polymorphic ‎DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships ‎among Salmone

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13

MOHANTY, S., K. RENUKA, S. SOOD, B. K. DAS, and A. KAPIL. "Antibiogram pattern and seasonality of Salmonella serotypes in a North Indian tertiary care hospital." Epidemiology and Infection 134, no. 5 (2006): 961–66. http://dx.doi.org/10.1017/s0950268805005844.

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The antibiogram pattern and seasonal distribution of Salmonella serotypes were analysed retrospectively over a 6-year period from January 1999 to December 2004. Blood cultures received in the Bacteriology Laboratory were processed by standard procedures and the Salmonella spp. isolates were identified with specific antisera and standard biochemical tests. Antimicrobial susceptibility testing was carried out by a standard disc diffusion method and the minimum inhibitory concentration (MIC) of ciprofloxacin for 332 representative Salmonella isolates was determined by E test. Salmonella Typhi (75

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Rödel, Jürgen, Birgit Edel, Sascha D. Braun, et al. "Simple differentiation of Salmonella Typhi, Paratyphi and Choleraesuis from Salmonella species using the eazyplex TyphiTyper LAMP assay." Journal of Medical Microbiology 69, no. 6 (2020): 817–23. http://dx.doi.org/10.1099/jmm.0.001201.

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Introduction. Identification of typhoidal Salmonella (TS) serovars and their discrimination from non-typhoidal Salmonella (NTS) is conventionally performed by seroagglutination. This method is labour-intensive, requires technical experience and can be inconclusive in some cases. Molecular assays may be reliable alternative diagnostic tools. Aim. This study was designed to evaluate the eazyplex TyphiTyper based on loop-mediated isothermal amplification (LAMP) for fast identification of TS and S. Choleraesuis in culture. Methodology. A total of 121 Salmonella strains and 33 isolates of other Ent

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15

Sajib, Mohammad S. I., Arif M. Tanmoy, Yogesh Hooda, et al. "Trends in antimicrobial resistance amongst Salmonella Paratyphi A isolates in Bangladesh: 1999–2021." PLOS Neglected Tropical Diseases 17, no. 11 (2023): e0011723. http://dx.doi.org/10.1371/journal.pntd.0011723.

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Background Typhoid and paratyphoid remain common bloodstream infections in areas with suboptimal water and sanitation infrastructure. Paratyphoid, caused by Salmonella Paratyphi A, is less prevalent than typhoid and its antimicrobial resistance (AMR) trends are less documented. Empirical treatment for paratyphoid is commonly based on the knowledge of susceptibility of Salmonella Typhi, which causes typhoid. Hence, with rising drug resistance in Salmonella Typhi, last-line antibiotics like ceftriaxone and azithromycin are prescribed for both typhoid and paratyphoid. However, unlike for typhoid,

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Amare, Azanaw, Fekadu Asnakew, Yonas Asressie, et al. "Prevalence of multidrug resistance Salmonella species isolated from clinical specimens at University of Gondar comprehensive specialized hospital Northwest Ethiopia: A retrospective study." PLOS ONE 19, no. 5 (2024): e0301697. http://dx.doi.org/10.1371/journal.pone.0301697.

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Background Multidrug resistance Salmonellosis remains an important public health problem globally. The disease is among the leading causes of morbidity and mortality in developing countries, but there have been limited recent studies about the prevalence, antimicrobial resistance, and multidrug resistance patterns of Salmonella isolates from various clinical specimens. Objective Aimed to assess the prevalence, antimicrobial resistance, and multidrug resistance patterns of Salmonella isolates from clinical specimens at the University of Gondar Comprehensive Specialised Hospital, northwestern Et

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LAN, TRAN T. Q., MARIE-LOU GAUCHER, NGUYEN T. M. NHAN, ANN LETELLIER, and SYLVAIN QUESSY. "Distribution of Virulence Genes among Salmonella Serotypes Isolated from Pigs in Southern Vietnam." Journal of Food Protection 81, no. 9 (2018): 1459–66. http://dx.doi.org/10.4315/0362-028x.jfp-17-408.

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ABSTRACT This study was carried out to evaluate the prevalence of Salmonella serogroups and serotypes and their virulence gene carriage in pig fecal samples from farms and slaughterhouses in some southern provinces of Vietnam. The presence of Salmonella was assessed based on culture enrichment of the collected samples and biochemical and serological analyses; 27.7% (51) of 184 samples were posititve for Salmonella. Based on the availability of antisera, serogroups were determined for 61% (31) of 51 isolates. Twenty isolates belonging to Salmonella serotypes Typhimurium (10 isolates), Anatum (8

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Patel, Falguni, and UV Shah. "A study of Isolation of Salmonella species from blood culture & it’s Antimicrobial Resistant Pattern." BJKines National Journal of Basic & Applied Sciences 15, no. 01 (2023): 10–15. http://dx.doi.org/10.56018/bjkines2023062.

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Background: The main aim of this study was to monitor the antimicrobial resistant pattern of Salmonella isolates to select proper antibiotic & prevent drug resistance in Salmonella species. Material and Methods: Total 250 blood culture bottles were collected from patients clinically suspected enteric fever and loaded to BACTEC 9050. Out of them 200 signalled positive samples were inoculated on chocolate and Mac Conkey agar plates and incubated overnight at 370 C in the incubator and identified by colony characteristics, procedures like Gram staining, wet preparation for motility and bio-ch

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Hossain, Md Mobarok, Tasnim Jabin, Md Ifrat Hossain, et al. "Antibiotic resistance profiling of clinical isolates of Salmonella enterica Serovar Paratyphi A in Dhaka, Bangladesh." Stamford Journal of Microbiology 11, no. 1 (2021): 14–16. http://dx.doi.org/10.3329/sjm.v11i1.57146.

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The study was conducted to detect the antibiotic resistance profile of the clinical isolates of Salmonella enterica Serovar Paratyphi A from 100 blood samples of patients from different age groups suspected to be suffering from enteric fever. The pure cultures of the bacterial isolates were collected from some renowned diagnostic centers of Dhaka and they were further characterized through the conventional culture, microscopy and biochemical examinations. These isolates were cross checked for the antibiogram profile by the Kirby-Bauer disc diffusion method against ten different types of antibi

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Ahmed, M. M., M. M. Rahman, K. R. Mahbub, and M. Wahiduzzaman. "Characterization of Antibiotic Resistant Salmonella spp Isolated from Chicken Eggs of Dhaka City." Journal of Scientific Research 3, no. 1 (2010): 191. http://dx.doi.org/10.3329/jsr.v3i1.6109.

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In present study, the occurrence of Salmonella in local chicken eggs and their pattern of antibiotic resistance were determined. 100 egg samples collected from different locations of Dhaka city were analysed and Salmonella spp were found in 8% of the samples. Among all presumptive Salmonella isolates, 8 isolates were confirmed as Salmonella enterica subsp. salamae (4%), Salmonella enterica subsp. indica (1%), Salmonella Paratyphi-A (1%), Salmonella bongori (1%) and Salmonella Choleraesuis (1%) on the basis of serotyping and biochemical analysis. These isolates were subjected to susceptibility

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Husna, Husna, Nurliana Nurliana, and Darmawi Darmawi. "The Presence of Salmonella in Sie Balu, Acehnese Dried Meat After Gamma Irradiation." International Journal of Tropical Veterinary and Biomedical Research 2, no. 1 (2017): 1–7. http://dx.doi.org/10.21157/ijtvbr.v2i1.8632.

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Sie balu is an Acehnese dried meat preserved by the addition of salt, acid and dried. However, long processing and drying it under the sun can cause meat products contaminated by Salmonella. Irradiation can eliminate Salmonella and other pathogenic bacteria in food. This study aims to determine the presence of Salmonella in sie balu after irradiated with increasing doses and 2 to 4 months shelf life. Sie balu was made of 5 kg fresh beef, dried under the sun to dry, vacuum and irradiated at doses of 5, 7 and 9 kGy. Salmonella was detected using bacteriological and biochemical tests. Results sho

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Agrawal, P., R. Tuladhar, and N. Dahal. "Nalidixic Acid Susceptibility Test for Screening Salmonella Isolates of Reduced Susceptibility/Higher Minimum Inhibitory Concentration to Ciprofloxacin." Nepal Journal of Science and Technology 15, no. 2 (2015): 97–104. http://dx.doi.org/10.3126/njst.v15i2.12122.

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Enteric fever is the major diagnosis among febrile patients in Nepal with yearly increase in nalidixic acid resistance and reduced ciprofloxacin susceptibility among Salmonella isolates. This study was carried out to evaluate the validity of nalidixic acid resistance as an indicator of reduced susceptibility of Salmonella isolates to ciprofloxacin. In this study, 999 blood specimens collected from suspected enteric fever patients visiting B&B Hospital were processed by standard microbiological techniques. Isolates were identified by biochemical tests and serotyping. Antibiotic susceptibili

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Chmel, Martin, Oldřich Bartoš, Ondřej Beran, et al. "Salmonella Paratyphi Infection: Use of Nanopore Sequencing as a Vivid Alternative for the Identification of Invading Bacteria." Prague Medical Report 122, no. 2 (2021): 96–105. http://dx.doi.org/10.14712/23362936.2021.10.

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In our study we present an overview of the use of Oxford Nanopore Technologies (ONT) sequencing technology on the background of Enteric fever. Unlike traditional methods (e.g., qPCR, serological tests), the nanopore sequencing technology enables virtually real-time data generation and highly accurate pathogen identification and characterization. Blood cultures were obtained from a 48-year-old female patient suffering from a high fever, headache and diarrhea. Nevertheless, both the initial serological tests and stool culture appeared to be negative. Therefore, the bacterial isolate from blood c

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Ijaz, Samia, Rifat Bibi, Nazia, et al. "Biochemical Characterization and Antibiotic Profile against Salmonella Species Isolated from Milk and Milk Products." Open Access Public Health and Health Administration Review 3, no. 2 (2025): 156–64. https://doi.org/10.59644/oaphhar.3(2).176.

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Salmonella species are among the most common foodborne pathogens associated with milk and dairy products. This study investigated the microbiological characteristics and antibiotic resistance patterns of Salmonella spp. in milk, ice cream, and cheese samples collected from August to December 2024 in Layyah District, Punjab, Pakistan. A total of 150 randomly selected samples (50 each of milk, ice cream, and cheese) were analyzed. Isolation and identification were performed using standard biochemical tests and further confirmed using the API 10S identification kit. Salmonella contamination was d

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ONO, Hiromi, Sakae IWAMOTO, Yoshinari AOKI, Ikuko TERADA, Yasuzumi YAMAMOTO, and Yasuzi NISHII. "An Outbreak of Food Poisoning due to Salmonella paratyphi-B (d-tartrate+) having Atypical Biochemical Property and Salmonella litchfield." Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 26, no. 3 (1985): 295–99. http://dx.doi.org/10.3358/shokueishi.26.295.

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Niroula, Dhirendra, Jyotsna Shrestha, Supriya Sharma, and Anjana Singh. "Antibiotic Susceptibility Pattern of Salmonella Enterica serovars Typhi and Paratyphi A Isolated From Patients Suspected of Enteric Fever." Tribhuvan University Journal of Microbiology 7 (December 26, 2020): 31–36. http://dx.doi.org/10.3126/tujm.v7i0.33791.

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Objectives: The study aimed to assess the antibiotic susceptibility profile of Salmonella spp isolated from patients suspected of enteric fever. Methods: This cross-sectional prospective study was carried out from April to June, 2014among 484 patients clinically suspected of enteric fever visiting Bir Hospital, Kathmandu, Nepal. Blood sample collected from each patient was processed for culture in bile broth. Identification of Salmonella spp was done by conventional microbiological techniques including colony characteristics, Gram's staining and biochemical tests. Antibiotic susceptibili

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Isah, Kamilu, and Sani Rabiu. "Incidence of <i>Salmonella</i> Bacteraemia among Patients Attending Selected Hospitals in Katsina State, Nigeria." HAFED POLY Journal of Science, Management and Technology 5, no. 2 (2024): 92–97. http://dx.doi.org/10.4314/hpjsmt.v5i2.7.

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Invasive Salmonellosis or Salmonella bacteremia is an infection cause by eating foods such as milk, eggs, vegetables, meat or water contaminated by faeces from an infected persons. It remains a threat to public health in many parts of the world especially developing countries where it becomes endemic. About 21.5 million cases occur per annum world-wide with 200,000 deaths in Asia, Africa and Latin America. About 80% of deaths due to Salmonella serovars occur in Africa. This research was conducted to find out the incidence of Salmonella bacteraemia among the patients attending selected hospital

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Shrestha, Esha, Dwij R. Bhatta, and Binod Lekhak. "Occurrence of Salmonella in drinking water samples of urban water supply system of Kathmandu." Botanica Orientalis: Journal of Plant Science 6 (March 15, 2010): 52–55. http://dx.doi.org/10.3126/botor.v6i0.2911.

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Water-borne diseases are among the leading causes of morbidity and mortality in developing countries and around 2.2 million people die every year due to basic hygiene-related diseases, like gastroenteritis, diarrhea, typhoid and dysentery. Eighty-six water samples were randomly collected from urban water supply system of Kathmandu, and analyzed for physiochemical and microbiological parameters to assess drinking water quality. Residual chlorine was undetectable in 100% samples. Salmonella was detected in 4 samples by enrichment culture technique in Selenite F broth followed by plating on Salmo

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Olorunsola, R. A., D. Eruvbetine, O. M. Idowu, O. Oyekunle, and I. M. Ogunade. "Salmonella organism transmission in hatching broiler egggs." Nigerian Journal of Animal Production 41, no. 1 (2021): 230–34. http://dx.doi.org/10.51791/njap.v41i1.2729.

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The vertical transmission of Salmonella organism in hatching broiler eggs were investigated in selected states in South-Western Nigeria. Hatching eggs were obtained from fifteen major hatcheries. five (hatchery A, B, C, D and E from each State [Lagos (LA), Oyo (OY) and Ogun (OG)]. A total of 300 hatchable eggs of 20 eggs from each hatchery were collected. Individual egg was broken and separated into Shell, albumin and yolk. The samples were incubated into buffer peptone water and incubated at 37°C for 24 hours. Typical colonies of salmonella grown on Xylose lysine deoxycholate agar were seen a

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Azad, Abul Kalam, Sahana Parveen, Md Shakir Uddin Ahmed, and Md Abdul Malek. "Occurrence, Antimicrobial Resistance Pattern and Plasmid Profile of Salmonella spp. Isolated from Raw Beef Meat in Dhaka City." Bangladesh Journal of Microbiology 29, no. 1 (2016): 17–22. http://dx.doi.org/10.3329/bjm.v29i1.28429.

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Salmonella is motile enterobacterium that causes diseases like typhoid fever, paratyphoid fever and food borne illnesses. Salmonella infections are due to the consumption of contaminated foods. The objective of the present study was to observe the sanitary and hygienic conditions of the retail areas and to isolate pathogenic Salmonella spp from raw beef, to observe antimicrobial sensitivity pattern of Salmonella spp from the raw beef. A total of forty (40) raw beef samples were collected from the sixteen different butcher shops of the four different markets namely Bazaar-1, Bazaar-2, Bazaar-3

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Fahima Akter and Md Shamimuzzaman. "DETECTION OF INVA GENE BY PCR AND IDENTIFICATION WITH SUSCEPTIBILITY PATTERN EVALUATION BY VITEK 2 OF SALMONELLA ISOLATES." Journal of Applied Biological Sciences 18, no. 3 (2024): 235–52. https://doi.org/10.71336/jabs.1313.

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This study aims to identify and antimicrobial susceptibility of hospital wastewater in Dhaka city. Total 134 Salmonella isolates were identified using cultural characteristics, automated biochemical test, and PCR for invA gene detection. Biochemical identification and Antimicrobial Susceptibility Test (AST) were performed through VITEK 2. Essential Agreement (EA) and Categorical Agreement (CA) were worked out according to the CLSI breakpoint. We also measured Very Major Error (VME), Major Error (ME), and Minor Error (mE). Seven Salmonella serotypes were identified through VITEK 2 ID-GN card an

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Al-Amin, M., MSR Khan, F. Begum, J. Hasan, and M. Asaduzzaman. "Isolation and Characterization of Bacterial Species from Enteric Fever Suspected Human Cases of Mymensingh Medical College Hospital." Progressive Agriculture 20, no. 1-2 (2013): 85–92. http://dx.doi.org/10.3329/pa.v20i1-2.16857.

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A total of 17 stool samples were collected from enteric fever suspected patients of Mymensingh Medical College Hospital, Mymensingh, Bangladesh. Results of morphological, biochemical examinations and pathogenicity test revealed Escherichia coli (47%), Klebsiella spp. (41%), Citrobacter spp. (29%). In cultural examination, all Escherichia coli showed metallic sheen on EMB agar, Klebsiella spp. showed moist shiny mucoid colonies and Citrobacter spp. showed large convex colorless dwarf colonies on MacConkey agar. In biochemical examination, all the isolates fermented five basic sugars with the pr

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Mahjaf, Ghanem Mohammed, Mosab Ahmed Alzain Mohammed, Hadia Abass Eltaib Ahmed, and Leila Mohamed A. Abdelgader. "In vitro assessment of antimicrobial activity of citrus lemon against selected clinical isolates from Shendi city, Sudan." Journal of Bacteriology & Mycology: Open Access 12, no. 3 (2024): 83–87. http://dx.doi.org/10.15406/jbmoa.2024.12.00378.

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Background: The high prevalence of microorganisms resistant to antimicrobials commonly used in clinical practice has impacted healthcare services due to reduced patient treatment options. Medicinal plants are thought to provide new sources of compounds that potentially replace antibiotics in the treatment of bacteria that are resistant to antibiotics. Objective: This work aimed at determining the antibacterial activity of Citrus lemon juice against gram-negative and gram-positive bacteria. Aims: This is a cross-sectional study in Shendi City, Sudan, from March to December 2023, at the microbio

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Akter, Fahima, and Md. Shamimuzzaman. "DETECTION OF INVA GENE BY PCR AND IDENTIFICATION WITH SUSCEPTIBILITY PATTERN EVALUATION BY VITEK 2 OF SALMONELLA ISOLATES." Journal of Applied Biological Sciences 18, no. 3 (2024): 235–52. https://doi.org/10.5281/zenodo.13857861.

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This study aims to identify and antimicrobial susceptibility of hospital wastewater in Dhaka city. Total 134 <em>Salmonella</em> isolates were identified using cultural characteristics, automated biochemical test, and PCR for <em>invA </em>gene detection. Biochemical identification and Antimicrobial Susceptibility Test (AST) were performed through VITEK 2. Essential Agreement (EA) and Categorical Agreement (CA) were worked out according to the CLSI breakpoint. We also measured Very Major Error (VME), Major Error (ME), and Minor Error (mE). Seven <em>Salmonella </em>serotypes were identified th

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Shoaib, Maria Ali, Usama Khalid Choudry, Iram Saddiqa Aamir, Qurrat-ul-Ain Aqeel, Syed Ahsan Uddin Ahmed, and Ghulam Fatima. "EMERGING RESISTANCE." Professional Medical Journal 23, no. 07 (2016): 822–27. http://dx.doi.org/10.29309/tpmj/2016.23.07.1647.

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Objectives: To study the bacteriological profile of enteric fever and their antibioticsensitivity pattern to commonly used antibiotics in view of emerging resistance at Civil HospitalKarachi (C.H.K.) and provide a guideline for making a protocol for empirical antibiotic therapywhere culture facilities are not available. Methodology: Design: Descriptive laboratory Basedstudy. Place and Duration of study: The study was conducted from 1st January, 2010 till 30thJune, 2012 at the Central Lab, Civil Hospital Karachi. Patients and Methods: A cross sectionalanalysis was done on a total of 37,805 bloo

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Lambertucci, José Roberto, Roberto Pedercini Marinho, Maria das Dores Ferreira, and Jayme Neves. "The value of the Widal test in the diagnosis of Prolonged Septicemic Salmonellosis." Revista do Instituto de Medicina Tropical de São Paulo 27, no. 2 (1985): 82–85. http://dx.doi.org/10.1590/s0036-46651985000200004.

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Twenty patients with prolonged septicemic salmonellosis (Group 1) and 20 with schistosomiasis mansoni (Group 2) were selected for this study. In both groups, the Widal test was done using antigens of the sample Ty 901 (S. typhi). The test was also applied in 6 group 1 patients with antigens prepared from salmonellae isolated from these patients (autoantigens). Titres over 1:200 were considered significant. Ten group 1 patients (50%) were positive for antigen "H" and 5 (25%) were positive for antigen "O". Three patients with negative "H" and "O" reactions became positive with high titres when u

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Bukhari, Hina, Tayyeba Komal, Raana Akhtar, Sami Ullah Mumtaz, and Iqra Waheed. "Recent Antimicrobial Susceptibility Patterns of Salmonella Isolates in A Tertiay Care Hospital Lahore." esculapio 17, no. 1 (2021): 45–48. http://dx.doi.org/10.51273/esc21.251719.

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Objective: To determine the recent antimicrobial susceptibility patterns of salmonella isolates (typhi and paratyphi) in a tertiary care hospital of Lahore. Methods: It is cross sectional retrospective study conducted out in King Edward Medical University(Pathology deptt)/Mayo Hospital Lahore.The study period is six months from May 2019 to October 2019(Peak months of Typhoid fever).During this six months study period, total of 4284 samples for blood culture were received that were inoculated on the macConkey and blood agar plates.The growths obtained were then processed through biochemical pro

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Tinrat, Sirikhwan, and Saranya Sedtananun. "NOVEL RUMMELIIBACILLUS SP. ISOLATED FROM FERMENTED VEGETABLE PRODUCTS AS THE POTENTIAL PROBIOTICS." Journal of microbiology, biotechnology and food sciences 11, no. 5 (2022): e4194. http://dx.doi.org/10.55251/jmbfs.4194.

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The novel lactic acid bacteria has potential probiotics which were selectively screened from different fermented vegetable products by antagonistic activity against Escherichia coli ATCC 25922 as a food-borne pathogenic bacteria. From 40 isolates on MRS agar, some isolates had anti-E. coli activity. Surprisingly, isolated STR 0103f and STR 0404f strains demonstrated bile salt-tolerance at 0.3% bile salt for 24 hours with survival rate of 67.77±0.38 and 68.72±0.45%, respectively. Both strains also survived under acidic conditions (pH 3-5) with 60.53±0.08-76.87±0.09% of survival rate. STR 0103f

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Ali, Mohammad Shaokat, Shamima Ahmed, Md Forhad Uddin, Abdul Matin, Mohammad Sharif Uddin, and Nilufa Yeasmin. "Characterization of Probiotic Properties of Lactic Acid Bacteria Isolated from Yoghurt Available in Bangladesh." Turkish Journal of Agriculture - Food Science and Technology 6, no. 11 (2018): 1544. http://dx.doi.org/10.24925/turjaf.v6i11.1544-1549.1856.

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Yoghurt is the common fermented dairy product consumed worldwide and it is considered a potential source of probiotic bacteria (Lactobacilli and Lactococci). To assess the in-vitro probiotic properties of lactic acid bacteria, Lactococcus lactis subsp. lactis, Lactobacillus delbrueckii and Lactobacillus plantarum species isolated from yoghurt sold in different local markets of Chittagong region in Bangladesh were examined by morphological characteristics and biochemical tests. In the preliminary test, all isolates were grown well at acidic pH (pH 3-5) while the optimum growth temperature was 3

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Baibhav, R. Barbaruah R. Roy U. Chetri and SR Joshi. "Currency notes in circulation can be potential vectors for transmissible diseases." NEHU International Journal l Vol. XIX, No. 2, (July –December), 2021 (2022): 82–96. https://doi.org/10.5281/zenodo.7098305.

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In the modern era, currency is the primary medium for exchange of goods and services. The material of Indian currency notes makes it a hub for microorganism to thrive. Taxi drivers exchange currency with customers for different regions and backgrounds and meat sellers are known to use bare hands for butchering of the meat and exchanging currency with customers without washing or sanitizing. This paper highlights the presence of multitude of bacteria which have the potential to cause diseases as well as being antibiotic resistant. 20 different strains were isolated, 10 each from currency notes

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Esonu, D. O., B. V. Maikai, and A. J. Oghumu. "Prevalence of Salmonella organisms in fresh and smoke-dried fish within parts of Kaduna metropolis, Kaduna State, Nigeria." Sokoto Journal of Veterinary Sciences 18, no. 1 (2020): 13–17. http://dx.doi.org/10.4314/sokjvs.v18i1.2.

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Aquatic environments are the major reservoirs of Salmonella and fishery products have been recognized as major carrier of food-borne pathogens. To determine the prevalence of Salmonella in fish, 112 fresh and smoke-dried fish samples were pre-enriched and enriched with buffered peptone water and selenite broth respectively, before plating on Desoxycholate Citrate Agar. Of the 112 samples, 75% (84/112) were contaminated with non-lactose fermenters. Upon subjecting them to biochemical tests, 13.1% (11/84) of the Salmonella suspects showed reactions consistent with that of Salmonella species, Pro

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Swaminathan, Chandrasekaran. "PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L." Asian Journal of Pharmaceutical and Clinical Research 11, no. 6 (2018): 136. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.20060.

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Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-

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Dauphin Dighitoghi Moro, Igbagbo Ehinmore, Oluchi Mariam Ukhureigbe, Jyostna Chandwani, and Kabiru Olusegun Akinyemi. "Serotyping and antibiotic susceptibility pattern of Salmonella serovars from children diagnosed of typhoid fever in Lagos, Nigeria." Open Access Research Journal of Science and Technology 4, no. 2 (2022): 058–65. http://dx.doi.org/10.53022/oarjst.2022.4.2.0034.

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Typhoid fever is a major health problem in developing countries especially in Nigeria where this infectious disease is endemic, with more concern in children. This prospective study was conducted in 3 Primary Health Centres on children diagnosed clinically of typhoid fever between January, 2017 and December, 2019. The aim of this study was to determine the incidence of Salmonella serovars and antibiotic susceptibility pattern from faecal specimens of children diagnosed clinically based on symptoms of typhoid fever, while demographic information was recovered from their case notes and their mot

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Keelan, Susan L., Russell S. Flowers, and Barbara J. Robison. "Multitest System for Biochemical Identification of Salmonella, Escherichia coli, and Other Enterobacteriaceae Isolated from Foods: Collaborative Study." Journal of AOAC INTERNATIONAL 71, no. 5 (1988): 968–72. http://dx.doi.org/10.1093/jaoac/71.5.968.

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Abstract Nine laboratories evaluated the ability of the MICRO-ID test system to correctly identify members of the Enterobacteriaceae. A total of 78 isolates representing 11 genera of enterics that had been previously isolated from foods were used in the collaborative study. The collaborators streaked each isolate on plate count agar and incubated the plates overnight at 35°C to check purity of the isolates. Then they proceeded with the method in which an isolated colony is transferred to a plate count agar slant and is incubated 18-24 h at 35°C. Growth from the slant is emulsified in 3.5 mL ph

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Manigundan, Kaari, Joseph Jerrine, Manikkam Radhakrishnan, Anbalmani Sivarajan, and Ramasamy Balagurunathan. "Multifaceted bioproperties of Streptomyces bacillaris ANS2 isolated from Andaman and Nicobar Islands, India." Research Journal of Biotechnology 16, no. 11 (2021): 99–108. http://dx.doi.org/10.25303/1611rjbt99108.

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The rich warehouse called marine actinobacteria has enticed researchers for several decades. The assessment of their metabolites has evidenced several diverse and multiple biological properties vacillating from antibacterial, antituberculosis, antioxidant, anticancer to mosquitocidal. Therefore, the present study has been aimed to reconnoitre the isolation and portrayal of the promising marine actinobacteria named Streptomyces bacillaris ANS2 isolated from the mangrove sediment collected from Andaman and Nicobar islands. The ethyl acetate extract of ANS2 showed broad spectrum activity against

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Horswill, Alexander R., and Jorge C. Escalante-Semerena. "Salmonella typhimurium LT2 Catabolizes Propionate via the 2-Methylcitric Acid Cycle." Journal of Bacteriology 181, no. 18 (1999): 5615–23. http://dx.doi.org/10.1128/jb.181.18.5615-5623.1999.

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ABSTRACT We previously identified the prpBCDE operon, which encodes catabolic functions required for propionate catabolism inSalmonella typhimurium. Results from13C-labeling experiments have identified the route of propionate breakdown and determined the biochemical role of each Prp enzyme in this pathway. The identification of catabolites accumulating in wild-type and mutant strains was consistent with propionate breakdown through the 2-methylcitric acid cycle. Our experiments demonstrate that the α-carbon of propionate is oxidized to yield pyruvate. The reactions are catalyzed by propionyl c

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Bakori, Hafsat sagir. "Prevalence of Non-Typhoidal Salmonella and Aerobic Bacteria Causing Bacteraemia among HIV/AIDS Seropositive Patients Attending Aminu Kano Teaching Hospital, Kano State." Sahel Journal of Life Sciences FUDMA 1, no. 1 (2023): 8–24. http://dx.doi.org/10.33003/sajols-2023-0101-002.

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The study aimed at determining the prevalence of non-typhoidal Salmonella and aerobic bacteria causing bacteraemia among HIV/AIDS seropositive patients attending Aminu Kano Teaching Hospital, Kano, the relationship between the CD4 cell counts and prevalence of these bacterial infections among the study population. Two hundred and sixty (260) HIV patients of 77 (29.6%) males and 183 (70.4%) females of 22-75 years were enrolled for the study. Socio-demographic and clinical history were obtained. The CD4 cell counts were enumerated using Cytoflometer. Bacteriological blood culture, isolation, ide

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Anelechi, Madubuike Stella, Mailafia Samuel Egwu O. Godwin, Olabode H. Olatunde, Momoh A.Habiba, Ode O. Julius, and Ramon-Yusuf S.Babatunde. "Phenotypic Identification of Citrobacter Isolates from Cloacal Swabs of Apparently Healthy Turtles at the River Banks in Lokoja, Kogi State, Nigeria." International Journal of Current Microbiology and Applied Sciences 11, no. 11 (2022): 117–25. http://dx.doi.org/10.20546/ijcmas.2022.1111.014.

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Citrobacter species are opportunistic pathogens in humans that can lead to invasive disease, including infections of the urinary tract, respiratory tract, Central Nervous System, skin, and soft tissues. A total of 245 cloacal swab samples were collected from the cloaca of apparently healthy turtles and subjected to conventional biochemical tests. Presumptive identification on MacConkey Agar (MAC) and Xylose lysine deoxycholate agar (XLD) yielded 42.0% (103) on each medium, on Eosin Methylene Blue agar (EMB) yielded 21.6 % (53), and on Salmonella-Shigella agar yielded 9.8% (24) isolates while I

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Borchert, Andrew J., Jacquelyn M. Walejko, Adrien Le Guennec, Dustin C. Ernst, Arthur S. Edison, and Diana M. Downs. "Integrated Metabolomics and Transcriptomics Suggest the Global Metabolic Response to 2-Aminoacrylate Stress in Salmonella enterica." Metabolites 10, no. 1 (2019): 12. http://dx.doi.org/10.3390/metabo10010012.

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In Salmonella enterica, 2-aminoacrylate (2AA) is a reactive enamine intermediate generated during a number of biochemical reactions. When the 2-iminobutanoate/2-iminopropanoate deaminase (RidA; EC: 3.5.99.10) is eliminated, 2AA accumulates and inhibits the activity of multiple pyridoxal 5’-phosphate(PLP)-dependent enzymes. In this study, untargeted proton nuclear magnetic resonance (1H NMR) metabolomics and transcriptomics data were used to uncover the global metabolic response of S. enterica to the accumulation of 2AA. The data showed that elimination of RidA perturbed folate and branched cha

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R., N. Patil, S. Kadam J., R. Ingole J., V. Sathe T., and D. Jadhav A. "Antibacterial activity of fish mucus from Clarias batrachus (Linn.) Against selected microbes." Biolife 3, no. 4 (2022): 788–91. https://doi.org/10.5281/zenodo.7302675.

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<strong>ABSTRACT</strong> Fish one of the diverse group of animals, that are highly specialized for their aquatic existence. Being fish are in aquatic intimate contact with such an environment that contains very high concentrations of bacteria and viruses. The immune system is composed of numerous organs and cells that act together in a dynamic network in the defense against infection, disease and foreign substances. Fish mucus were tested by using disc diffusion technique against seven pathogenic bacteria such as <em>Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aerugenosa, Proteu

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