Journal articles: 'Sample calling'

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Marsh, Dylan R., Alexandra J. Alayan, and Bryan J. Dik. "Answered Callings, Unanswered Callings, or No Calling: Examining a Nationally Representative Sample." Career Development Quarterly 68, no. 4 (December 2020): 374–80. http://dx.doi.org/10.1002/cdq.12243.

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Nielsen, Rasmus, Thorfinn Korneliussen, Anders Albrechtsen, Yingrui Li, and Jun Wang. "SNP Calling, Genotype Calling, and Sample Allele Frequency Estimation from New-Generation Sequencing Data." PLoS ONE 7, no. 7 (July 24, 2012): e37558. http://dx.doi.org/10.1371/journal.pone.0037558.

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Lynch, Andy G., Suet-Feung Chin, Mark J. Dunning, Carlos Caldas, Simon Tavaré, and Christina Curtis. "Calling Sample Mix-Ups in Cancer Population Studies." PLoS ONE 7, no. 8 (August 9, 2012): e41815. http://dx.doi.org/10.1371/journal.pone.0041815.

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Park, Jiyoung, and Young Woo Sohn. "Is it happy to work with leaders viewing their work as a calling?" Korean Journal of Industrial and Organizational Psychology 31, no. 2 (May 31, 2018): 353–85. http://dx.doi.org/10.24230/kjiop.v31i2.353-385.

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Although scholars have paid increasing attention to people with callings, relationships between leader's calling and follower's job attitudes have been understudied. The purpose of this study was to investigate the relationship between leader calling and follower job satisfaction, and mediators and a moderator on the relationship. We hypothesized that leader calling would be positively related to follower job satisfaction via follower's perceived transformational leadership and occupational self-efficacy and that the two mediators would be positively related. As a boundary condition, we tested a moderating role of job crafting on the positive relationship between leader calling and follower occupational self-efficacy. To examine the hypotheses, we conducted two survey studies using a sample of 242 Korean working adults (Study 1) and a sample of 221 American working adults in diverse industries (Study 2). We found a positive relationship between leader calling and follower job satisfaction (Study 1) and a significant mediating effect of transformational leadership on the relationship (Studies 1, 2). In both studies, follower occupational self-efficacy mediated the link between transformational leadership and follower job satisfaction rather than directly mediating the relationship between leader calling and follower job satisfaction. Also, when followers were highly involved in job crafting (Study 1) and cognitive crafting (Study 2), their occupational self-efficacy did not vary depending on the level of leader calling. Interestingly, the relationship between leader calling and follower occupational self-efficacy was negative when followers showed low levels of job crafting and cognitive crafting. We discuss the implications of these results, study limitations, and practical suggestions for future research.

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Bedo, Justin, Benjamin Goudey, Jeremy Wazny, and Zeyu Zhou. "Information theoretic alignment free variant calling." PeerJ Computer Science 2 (July 25, 2016): e71. http://dx.doi.org/10.7717/peerj-cs.71.

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While traditional methods for calling variants across whole genome sequence data rely on alignment to an appropriate reference sequence, alternative techniques are needed when a suitable reference does not exist. We present a novel alignment and assembly free variant calling method based on information theoretic principles designed to detect variants have strong statistical evidence for their ability to segregate samples in a given dataset. Our method uses the context surrounding a particular nucleotide to define variants. Given a set of reads, we model the probability of observing a given nucleotide conditioned on the surrounding prefix and suffixes of lengthkas a multinomial distribution. We then estimate which of these contexts are stable intra-sample and varying inter-sample using a statistic based on the Kullback–Leibler divergence.The utility of the variant calling method was evaluated through analysis of a pair of bacterial datasets and a mouse dataset. We found that our variants are highly informative for supervised learning tasks with performance similar to standard reference based calls and another reference free method (DiscoSNP++). Comparisons against reference based calls showed our method was able to capture very similar population structure on the bacterial dataset. The algorithm’s focus on discriminatory variants makes it suitable for many common analysis tasks for organisms that are too diverse to be mapped back to a single reference sequence.

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Zhang, Chunyu, Andreas Hirschi, Anne Herrmann, Jia Wei, and Jinfu Zhang. "Self-directed career attitude as predictor of career and life satisfaction in Chinese employees." Career Development International 20, no. 7 (November 9, 2015): 703–16. http://dx.doi.org/10.1108/cdi-06-2015-0090.

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Purpose – The purpose of this paper is to test if the effects of a self-directed career attitude on career and life satisfaction are mediated by a person’s sense of calling and moderated by job insecurity in a sample of Chinese employees. Design/methodology/approach – Among a sample of Chinese employees (n=263), in this paper, a moderated mediation analysis with bootstrapping was applied to test the hypotheses. Findings – The results showed that calling mediates the effects of a self-directed career attitude on career satisfaction and life satisfaction. Job insecurity moderated the effect on life satisfaction but not on career satisfaction. The effect on life satisfaction were stronger under higher levels of job insecurity. Research limitations/implications – These results suggest that a self-directed career attitude may help people develop a calling, which in turn relates to increased subjective career success and well-being. In addition, the notion of a calling may be especially important for well-being in unstable job circumstances. Originality/value – This study is the first to explore a calling and a self-directed career attitude in a sample of Chinese employees. Corresponding to contemporary China’s rapidly changing context of economy and career development, a self-directed career orientation plays an important role in Chinese employees’ calling and subjective career success.

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Davidson, Debra J., and Richard C. Stedman. "Calling forth the change-makers." Acta Sociologica 61, no. 1 (March 2, 2017): 79–94. http://dx.doi.org/10.1177/0001699317693065.

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Reflexivity theory can contribute in important ways to our understanding of how societies contend with climate change. Avoiding the catastrophic effects of “dangerous climate change” will require substantial change, yet emissions continue to rise. Social scientific research on climate change mitigation is dominated by a relatively small number of macro-theoretical frameworks, plus a rather large and disparate empirical literature on individual cognitions, attitudes and behaviors. We apply Archer’s reflexivity theory to a survey sample drawn from Alberta, Canada, showing that unique predispositions toward reflexivity offer strong predictors of willingness to engage in climate-related mitigative behaviors.

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Zhang, Chunyu, Andreas Hirschi, and Xuqun You. "Trajectories of Calling in the Transition From University to Work: A Growth Mixture Analysis." Journal of Career Assessment 29, no. 1 (July 3, 2020): 98–114. http://dx.doi.org/10.1177/1069072720931010.

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Research on the development of calling is still in its infancy and rarely focused on how calling changes during a major career transition. The current study examined the developmental trajectories of calling and their relation with personality (i.e., conscientiousness, proactive personality) in the transition from university to work with a three-wave longitudinal study with 340 Chinese graduating university students. Results based on growth mixture modeling indicated three developmental trajectories of calling: high and stable calling (23% of sample), high but decreasing calling (74%), and low and increasing calling (3%). Moreover, higher conscientiousness related to a higher chance of being classified into the high and stable calling trajectory. These findings add notable insights to the literature by exploring the previously neglected developmental trajectories of calling and their association with personality in the transition from university to work.

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Crozier, W. Ray, and Patricia S. Dimmock. "Name-calling and nicknames in a sample of primary school children." British Journal of Educational Psychology 69, no. 4 (December 1999): 505–16. http://dx.doi.org/10.1348/000709999157860.

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Darby, Charlotte A., James R. Fitch, Patrick J. Brennan, Benjamin J. Kelly, Natalie Bir, Vincent Magrini, Jeffrey Leonard, et al. "Samovar: Single-Sample Mosaic Single-Nucleotide Variant Calling with Linked Reads." iScience 18 (August 2019): 1–10. http://dx.doi.org/10.1016/j.isci.2019.05.037.

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Flickinger, Matthew, Goo Jun, Gonçalo R. Abecasis, Michael Boehnke, and Hyun Min Kang. "Correcting for Sample Contamination in Genotype Calling of DNA Sequence Data." American Journal of Human Genetics 97, no. 2 (August 2015): 284–90. http://dx.doi.org/10.1016/j.ajhg.2015.07.002.

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Abbas, Nida. "EMERGENCE OF WORKPLACE SPIRITUALITY AS AN OUTCOME OF CALLING WITH A MODERATING ROLE OF CAREER COMMITMENT AMONG MEDICAL PROFESSIONALS IN PAKISTAN." Jinnah Business Review 03, no. 01 (January 1, 2015): 67–76. http://dx.doi.org/10.53369/wact9250.

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The study focused on the emergence of workplace spirituality as an outcome of calling, among medical professionals in Pakistan. Some of the main determinants of calling identified in this research were passion, occupational self-efficacy and work meaningfulness. The data was collected from a sample of 200 participants by using questionnaires. Results showed that calling was positively related with workplace spirituality and passion. On the other hand, calling was not found to be associated with occupational self-efficacy and work meaningfulness. Calling was found to be mediating the relationship of passion, occupational self-efficacy and work meaningfulness with workplace spirituality. However, career commitment was not found to be moderating the relationship of calling with workplace spirituality.

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Vianello, Michelangelo, Elisa Maria Galliani, Anna Dalla Rosa, and Pasquale Anselmi. "The Developmental Trajectories of Calling: Predictors and Outcomes." Journal of Career Assessment 28, no. 1 (February 28, 2019): 128–46. http://dx.doi.org/10.1177/1069072719831276.

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There are many open questions concerning the development of calling, and longitudinal empirical evidence is limited. We know that a calling is associated with many beneficial outcomes, but we do not know how it changes through time and what predicts these changes. Previous studies have shown that calling is relatively stable at the sample level. We show that, at the individual level, calling shows huge variations through time. We identified nine developmental trajectories that are typical across facets of calling, and we found evidence that the development of a calling is fostered by the extent to which individuals have lived it out. We also observed that the more a calling has grown over a 2-year period, the more it is lived out during the third year. These results provide support for a developmental model of calling according to which having a calling and living it out reciprocally influence each other. The practical and theoretical implications of these results are discussed.

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Li, Hongxia, and Xiugang Yang. "When a calling is living." Journal of Chinese Human Resource Management 9, no. 2 (October 8, 2018): 77–106. http://dx.doi.org/10.1108/jchrm-12-2017-0030.

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Purpose The argument that work engagement enhances job performance has gained wide acceptance among practitioners and human resources management literature. There is consensus in management literature that job crafting can affect work engagement. The concept of callings from theology has been resurrected in job behavior and continues to garner growing attention from practitioners in recent years. However, few studies examine how and why living a calling influence job crafting and work engagement. The purpose of this paper is to examine the relationships between living a calling, job crafting and work engagement for knowledgeable employees through questionnaires. Design/methodology/approach The part-time MBA students were asked to reflect on present jobs. In total, 390 effective questionnaires were collected from part-time MBA students of four universities in Chongqing, China for finance, administration, manufacturing, service, technology, medication, education and others. Results were analyzed using SPSS and Amos. The measurement scale is given in Appendix. Findings First, the author explicitly proposes and validates the direct relationship between living a calling and job crafting. Second, this study confirms that crafting challenging job demands are significant to vigor subdimension and dedication subdimension of work engagement, whereas crafting challenging job demands not significant to absorption subdimension of work engagement. Third, this study indicates that crafting hindering job demands are nonsignificant to vigor, dedication and absorption about three subdimensions of work engagement. Fourth, this study showed living a calling can enhance work engagement for employees. Fifth, this study finds three groups (eight items) of mediation effect between living a calling, job crafting and work engagement. Practical implications These insights may help managers to focus on living a calling and encourage beneficial job crafting behaviors in China. The sample is original and has the potential to contribute to debate on work life balance and particularly the meaning of work/careers in China. Social implications This study is an interesting revisit to the old workplace sociology and organizational psychology which has become somewhat neglected these days. Originality/value This study has provided insight in the relationships between living a calling, job crafting and work engagement.

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Kumar, Pankaj, Mashael Al-Shafai, Wadha Al Muftah, Nader Chalhoub, Mahmoud F. Elsaid, Alice Aleem, and Karsten Suhre. "Evaluation of SNP calling using single and multiple-sample calling algorithms by validation against array base genotyping and Mendelian inheritance." BMC Research Notes 7, no. 1 (2014): 747. http://dx.doi.org/10.1186/1756-0500-7-747.

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Dumulescu, Daniela, Adrian Opre, and Buzgar Ramona. "“Is Your Career Meaningful?” Exploring Career Calling on a Romanian Students Sample." Procedia - Social and Behavioral Sciences 187 (May 2015): 553–58. http://dx.doi.org/10.1016/j.sbspro.2015.03.103.

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Saunders, Christopher T., Wendy S. W. Wong, Sajani Swamy, Jennifer Becq, Lisa J. Murray, and R. Keira Cheetham. "Strelka: accurate somatic small-variant calling from sequenced tumor–normal sample pairs." Bioinformatics 28, no. 14 (May 10, 2012): 1811–17. http://dx.doi.org/10.1093/bioinformatics/bts271.

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Dumulescu, Daniela, Robert Balazsi, Alexandra Manuil, and Adrian Opre. "The Validation of Multidimensional Calling Measure on a sample of Romanian teachers." Cognition, Brain, Behavior. An interdisciplinary journal 23, no. 3 (September 16, 2019): 229–43. http://dx.doi.org/10.24193/cbb.2019.23.13.

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Goštautaitė, Bernadeta, Ilona Bučiūnienė, Anna Dalla Rosa, Ryan Duffy, and Haram Julia Kim. "Healthcare professionals with calling are less likely to be burned out: the role of social worth and career stage." Career Development International 25, no. 6 (October 1, 2020): 649–70. http://dx.doi.org/10.1108/cdi-10-2018-0255.

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PurposeThe association of calling with burnout is not well understood. This study investigates how calling influences burnout and what the roles of social worth and career stage are in this relation. Drawing from the Conservation of Resources Theory, we expect that calling may be negatively associated with burnout through increased social worth and that career stage moderates these relationships.Design/methodology/approachBased on a sample of 566 healthcare professionals, we conducted regression analyses with bootstrapping procedures to test the proposed hypotheses.FindingsThe findings show that social worth mediates the negative relation between calling and burnout. Additionally, the positive relation between calling and social worth was more pronounced for late-career employees; yet, the negative relation between social worth and burnout was stronger for early-career employees.Practical implicationsThe findings suggest that searching and pursuing a professional calling is beneficial for individuals. Additionally, social worth is crucial in this relation and could be used to actively prevent burnout.Originality/valueThe study advances our understanding of the consequences of calling for employees by explaining the underlying mechanism between calling and burnout and its importance at different career stages.

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Autin, Kelsey L., Blake A. Allan, Meenakshi Palaniappan, and Ryan D. Duffy. "Career Calling in India and the United States." Journal of Career Assessment 25, no. 4 (September 1, 2016): 688–702. http://dx.doi.org/10.1177/1069072716665860.

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The present study examined the cross-cultural validity of instruments used to assess the experience of searching for, having, and living out a calling. Using a sample of 336 Americans and 327 Indians, we used structural equation modeling to assess measurement invariance of three common scales used to measure calling: the Calling and Vocation Questionnaire (CVQ), the Brief Calling Scale, and the Living Calling Scale. Results showed partial measurement equivalence for the presence scale of the CVQ, indicating that it may be a valid measurement of within-group differences among Indian participants. Analyses on remaining scales showed borderline support for equivalence of factor structure and failed to demonstrate validity of cross-cultural comparisons. Implications for researchers and clinical practitioners are discussed.

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Lau, Poh Li, Kerrie G. Wilkins-Yel, and Y. Joel Wong. "Examining the Indirect Effects of Self-Concept on Work Readiness Through Resilience and Career Calling." Journal of Career Development 47, no. 5 (May 19, 2019): 551–64. http://dx.doi.org/10.1177/0894845319847288.

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The current study examined the relations among self-concept, career calling, resilience, and work readiness in a Malaysian sample of vocational college students ( N = 674). As hypothesized, self-concept was positively associated with work readiness. To explain this relation further, we examined the indirect effect of self-concept through calling and resilience on work readiness. Using structural equation modeling, both career calling and resilience were found to be significant avenues through which self-concept influences work readiness. Results of the indirect effects also indicated that resilience was a stronger pathway compared to career calling. These results suggest that for vocational college students, a more crystallized self-concept may link to greater work readiness skills due, in part, to a heightened sense of resilience and clarity in one’s career calling.

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FU, BILIN, and JIN XU. "A NEW GENOTYPE CALLING METHOD FOR AFFYMETRIX SNP ARRAYS." Journal of Bioinformatics and Computational Biology 09, no. 06 (December 2011): 715–28. http://dx.doi.org/10.1142/s0219720011005458.

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Current genotype-calling methods such as Robust Linear Model with Mahalanobis Distance Classifier (RLMM) and Corrected Robust Linear Model with Maximum Likelihood Classification (CRLMM) provide accurate calling results for Affymetrix Single Nucleotide Polymorphisms (SNP) chips. However, these methods are computationally expensive as they employ preprocess procedures, including chip data normalization and other sophisticated statistical techniques. In the small sample case the accuracy rate may drop significantly. We develop a new genotype calling method for Affymetrix 100 k and 500 k SNP chips. A two-stage classification scheme is proposed to obtain a fast genotype calling algorithm. The first stage uses unsupervised classification to quickly discriminate genotypes with high accuracy for the majority of the SNPs. And the second stage employs a supervised classification method to incorporate allele frequency information either from the HapMap data or from a self-training scheme. Confidence score is provided for every genotype call. The overall performance is shown to be comparable to that of CRLMM as verified by the known gold standard HapMap data and is superior in small sample cases. The new algorithm is computationally simple and standalone in the sense that a self-training scheme can be used without employing any other training data. A package implementing the calling algorithm is freely available at .

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Sun, Li, and Ning Cheng. "Impacts of Sense of Career Calling and Perceived Career Development Opportunities on Work Engagement: A Case Study of Undergraduate Academic Advisor." International Journal of Business and Management 16, no. 8 (June 26, 2021): 37. http://dx.doi.org/10.5539/ijbm.v16n8p37.

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This article studies the impacts of sense of career calling and perception of career development opportunities on work engagement, taking academic advisors as survey object. An online questionnaire survey was carried out on academic advisors of colleges and universities in Henan Province, China. Purposive sampling is used for the convenience of data collection, and a total of 400 valid questionnaires were received. SPSS statistical software is used to conduct descriptive analysis, independent sample t-test, and regression analysis of sample data. Research results show that academic advisors have a higher sense of career calling, perception of career development opportunities, and work engagement. Married teachers have a higher sense of career calling, perception of career development opportunities, and work engagement. Sense of career calling and perceived career development opportunities have a significant positive impact on work engagement, and career mission has a greater impact on work engagement than perceived career development opportunities. This research can help college administrators and decision makers realize the importance of the psychological needs of academic advisors in improving work engagement.

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Cline, Eliot, Nuttachat Wisittipanit, Tossapon Boongoen, Ekachai Chukeatirote, Darush Struss, and Anant Eungwanichayapant. "Recalibration of mapping quality scores in Illumina short-read alignments improves SNP detection results in low-coverage sequencing data." PeerJ 8 (December 7, 2020): e10501. http://dx.doi.org/10.7717/peerj.10501.

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Background Low-coverage sequencing is a cost-effective way to obtain reads spanning an entire genome. However, read depth at each locus is low, making sequencing error difficult to separate from actual variation. Prior to variant calling, sequencer reads are aligned to a reference genome, with alignments stored in Sequence Alignment/Map (SAM) files. Each alignment has a mapping quality (MAPQ) score indicating the probability a read is incorrectly aligned. This study investigated the recalibration of probability estimates used to compute MAPQ scores for improving variant calling performance in single-sample, low-coverage settings. Materials and Methods Simulated tomato, hot pepper and rice genomes were implanted with known variants. From these, simulated paired-end reads were generated at low coverage and aligned to the original reference genomes. Features extracted from the SAM formatted alignment files for tomato were used to train machine learning models to detect incorrectly aligned reads and output estimates of the probability of misalignment for each read in all three data sets. MAPQ scores were then re-computed from these estimates. Next, the SAM files were updated with new MAPQ scores. Finally, Variant calling was performed on the original and recalibrated alignments and the results compared. Results Incorrectly aligned reads comprised only 0.16% of the reads in the training set. This severe class imbalance required special consideration for model training. The F1 score for detecting misaligned reads ranged from 0.76 to 0.82. The best performing model was used to compute new MAPQ scores. Single Nucleotide Polymorphism (SNP) detection was improved after mapping score recalibration. In rice, recall for called SNPs increased by 5.2%, while for tomato and pepper it increased by 3.1% and 1.5%, respectively. For all three data sets the precision of SNP calls ranged from 0.91 to 0.95, and was largely unchanged both before and after mapping score recalibration. Conclusion Recalibrating MAPQ scores delivers modest improvements in single-sample variant calling results. Some variant callers operate on multiple samples simultaneously. They exploit every sample’s reads to compensate for the low read-depth of individual samples. This improves polymorphism detection and genotype inference. It may be that small improvements in single-sample settings translate to larger gains in a multi-sample experiment. A study to investigate this is ongoing.

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Sirine, Hani, and Elisabeth Penti Kurniawati. "The importance of spirituality dimensions in the development of entrepreneurship." Diponegoro International Journal of Business 1, no. 2 (November 17, 2018): 55. http://dx.doi.org/10.14710/dijb.1.2.2018.55-70.

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Human being as spiritual being needs to be nurtured spiritually so that they can be optimal in their work and their calling. Actualization of this calling can be made through entrepreneurship. The purpose of this study is to determine the importance of spiritual dimensions (vision, faith/hope, love altruistic, meaning/calling, membership) in the development of entrepreneurship (entrepreneurship intention, entrepreneurial networking, entrepreneurial capability, and entrepreneurial success). The analysis technique of this study uses multiple linear regressions with a sample of 67 graduates of Universitas Kristen Satya Wacana (UKSW) who have had and run their own business. The results show that the spirituality dimensions (vision, faith/hope, altruistic love, meaning/calling, membership) significantly influence entrepreneurial intention, entrepreneurial networking, entrepreneurial capability, and entrepreneurial success. When it is tested partially, the significant positive effect on entrepreneurial intention is vision and faith/hope. Faith/hope also has significant positive effects on entrepreneurial networking. Vision, faith/hope, and meaning/calling has significant positive effects on entrepreneurial capabilities. Lastly, faith/hope, and meaning/calling has significant positive effects on entrepreneurial success.

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Li, H. "Exploring single-sample SNP and INDEL calling with whole-genome de novo assembly." Bioinformatics 28, no. 14 (May 7, 2012): 1838–44. http://dx.doi.org/10.1093/bioinformatics/bts280.

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Fleury, Ruth E., Cris M. Sullivan, Deborah I. Bybee, and William S. Davidson. "Why Don’t They Just Call the Cops?: Reasons for Differential Police Contact Among Women with Abusive Partners." Violence and Victims 13, no. 4 (January 1998): 333–46. http://dx.doi.org/10.1891/0886-6708.13.4.333.

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Incidents of domestic violence are frequently not reported to police (e.g., Johnson, 1990; Langan & Innes, 1986; Roy, 1977), and people commonly assume that women’s reasons for not calling about violence by a current or former partner are intrapersonal (e.g., shame, embarrassment, love). However, few researchers have asked battered women themselves about the frequency of their police contacts and their reasons for not calling the police. In this study, participants were recruited from a battered women’s shelter and asked about their experiences with the police over the prior 6 months. Two thirds of the sample had had contact with the police during that time, but most did not have as much contact with the police as they had needed. Women gave multiple reasons for not calling the police; these reasons frequently included situational barriers, such as being physically prevented from using the telephone and/or being threatened with more violence. Only 3% of the sample reported that shame, embarrassment, or love were their sole reasons for not calling the police. Underreporting was related to previous (negative) experience with the police, as well as to the level of violence experienced. The practical implications of these findings are discussed.

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Lee, Hang-Shim, Eun Sul Lee, and Yun-Jeong Shin. "The Role of Calling in a Social Cognitive Model of Well-Being." Journal of Career Assessment 28, no. 1 (January 29, 2019): 59–75. http://dx.doi.org/10.1177/1069072719825777.

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The present study examined the role of calling in a social cognitive model of well-being using a sample of 328 South Korean teachers. The model incorporating calling into the social cognitive model of well-being demonstrated an excellent fit, and our variables accounted for significant variance in job satisfaction (47%) and life satisfaction (38%). Among the 12 direct paths of the proposed model, 10 hypothesized paths were significant. The direct paths from positive affect to calling, self-efficacy, job satisfaction, and life satisfaction; from calling to self-efficacy, outcome expectations, and life satisfaction; from self-efficacy to outcome expectations; from outcome expectations to job satisfaction; and from job satisfaction to life satisfaction were significant. Additionally, the mediating paths between positive affect and life satisfaction via calling, self-efficacy, outcome expectations, and job satisfaction were significant. The practical implications for enhancing teachers’ job and life satisfaction and future directions of research were discussed.

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Cain, Lisa, James Busser, and Hee Jung (Annette) Kang. "Executive chefs’ calling: effect on engagement, work-life balance and life satisfaction." International Journal of Contemporary Hospitality Management 30, no. 5 (May 14, 2018): 2287–307. http://dx.doi.org/10.1108/ijchm-02-2017-0105.

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PurposeThis paper aims to understand the relationships among calling, employee engagement, work-life balance and life satisfaction for executive chefs based on role theory and spillover theory.Design/methodology/approachSurveys were completed by members of the American Culinary Federation in North America, the Nevada Restaurant Association and attendees at the ChefConnect Annual Conference. The data were analysed with confirmatory factor analysis and structural equation modelling.FindingsAll relationships in the model were significantly positive except for calling to life satisfaction. Importantly work-life balance was a significant mediator between calling and life satisfaction as well as for employee engagement and life satisfaction.Research limitations/implicationsThe research provides a more comprehensive framework for hospitality scholars to understand the outcomes of work as a calling through meaningfulness. The sample of executive chef limits generalizability.Practical implicationsThe identification of a calling through in-depth interviews is recommended. Once recognized, managers should further foster chef’s passion through employee engagement facilitated by workplace autonomy and continuing education and work-life balance supported with human resource management practices including time off for critical life events. This will allow calling to flourish, increase life satisfaction and reduce the likelihood of turnover and burnout.Originality/valueOutcomes reveal the complexity of the relationship between calling and life satisfaction. Contrary to previous findings, the presence of positive work-life balance was critical to attain life satisfaction, even when work was viewed as a calling.

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Vicente, Paula. "The Best Times to Call in a Mobile Phone Survey." International Journal of Market Research 57, no. 4 (July 2015): 555–70. http://dx.doi.org/10.2501/ijmr-2015-047.

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Establishing contact with the sample units is an important part of the survey response process, and an efficient calling schedule is critical to achieve high response rates. The rapid increase in mobile phone ownership has triggered the interest of marketing researchers in the use of mobile phones for collecting survey data about consumers. Mobile phone surveys may favour establishing contact with sample units since the mobile phone is a personal device carried at all times, thus making the person permanently contactable. This paper aims to identify the best times to call in a mobile phone survey by investigating the influence of the day and time of the call on the likelihood of establishing contact and obtaining an interview. A three-level ranking of calling periods, based on call efficiency, is proposed. Outcomes also revealed that the level of efficiency of calling periods is not dissociated from respondents’ socio-demographic characteristics, namely in terms of age and region of residence.

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Derryberry, Dakota Z., Matthew C. Cowperthwaite, and Claus O. Wilke. "Reproducibility of SNV-calling in multiple sequencing runs from single tumors." PeerJ 4 (January 4, 2016): e1508. http://dx.doi.org/10.7717/peerj.1508.

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We examined 55 technical sequencing replicates of Glioblastoma multiforme (GBM) tumors from The Cancer Genome Atlas (TCGA) to ascertain the degree of repeatability in calling single-nucleotide variants (SNVs). We used the same mutation-calling pipeline on all pairs of samples, and we measured the extent of the overlap between two replicates; that is, how many specific point mutations were found in both replicates. We further tested whether additional filtering increased or decreased the size of the overlap. We found that about half of the putative mutations identified in one sequencing run of a given sample were also identified in the second, and that this percentage remained steady throughout orders of magnitude of variation in the total number of mutations identified (from 23 to 10,966). We further found that using filtering after SNV-calling removed the overlap completely. We concluded that there is variation in the frequency of mutations in GBMs, and that while some filtering approaches preferentially removed putative mutations found in only one replicate, others removed a large fraction of putative mutations found in both.

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Lowy, Ernesto, Susan Fairley, and Paul Flicek. "Variant calling across 505 openly consented samples from four Gambian populations on GRCh38." Wellcome Open Research 6 (September 16, 2021): 239. http://dx.doi.org/10.12688/wellcomeopenres.17001.1.

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The International Genome Sample Resource (IGSR) repository was established to maximise the utility of human genetic data derived from openly consented samples within the research community. Here we describe variant detection in 505 samples from four populations in The Gambia, using the GRCh38 reference genome, adding to the range of populations for which this has been done and, importantly, making allele frequencies available. A multi-caller site discovery process was applied along with imputation and phasing to produce a phased biallelic single nucleotide variant (SNV) and insertion/deletion (INDEL) call set. Variation had not previously been explored on the GRCh38 human genome assembly for 387 of the samples. Compared to our previous work with the 1000 Genomes Project data on GRCh38, we identified over nine million novel SNVs and over 870 thousand novel INDELs.

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Bizon, Chris, Michael Spiegel, Scott A. Chasse, Ian R. Gizer, Yun Li, Ewa P. Malc, Piotr A. Mieczkowski, et al. "Variant calling in low-coverage whole genome sequencing of a Native American population sample." BMC Genomics 15, no. 1 (2014): 85. http://dx.doi.org/10.1186/1471-2164-15-85.

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Monzon, Sara, Javier Alonso, Gonzalo Gómez, David Gonzalez-Pisano, and Isabel Cuesta. "Comparison of variant calling methods in exome sequencing of matched tumor-normal sample pairs." EMBnet.journal 19, A (April 8, 2013): 62. http://dx.doi.org/10.14806/ej.19.a.659.

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Denny, Elaine K., and Jesse Driscoll. "Calling Mogadishu: How Reminders of Anarchy Bias Survey Participation." Journal of Experimental Political Science 6, no. 02 (August 29, 2018): 81–92. http://dx.doi.org/10.1017/xps.2018.20.

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AbstractHow does the fear of anarchy affect telephone survey behaviors? A survey experiment administered to a sample of Mogadishu residents—validated with a natural experiment—is used to assess this question. Randomly assigned reminders of anarchic violence conditioned differential effects on survey participation depending on subjects’ background level of security and welfare. Vulnerable subjects were more likely than non-vulnerable subjects to refuse to provide sensitive survey information after reminders of anarchy.

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Gwak, YeonSun, Yeseul Jung, and YoungWoo Sohn. "Who suffers the most from abusive supervision?" Korean Journal of Industrial and Organizational Psychology 30, no. 3 (August 31, 2017): 443–64. http://dx.doi.org/10.24230/kjiop.v30i3.443-464.

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We examined the interactive effects of calling and competitive climate on the relationship between abusive supervision and turnover intention via organizational cynicism. We hypothesized that high levels of calling would strengthen, and high levels of competitive climate would mitigate, the negative indirect effects of abusive supervision on turnover intention via organizational cynicism. We conducted a survey using a sample of U.S. employees (N = 236) to test five hypotheses. As predicted, results demonstrate that under a high level of abusive supervision, individuals were more likely to become cynical about the organization, which in turn heightened turnover intention. Moderated mediation analyses found that calling exacerbated, but competitive climate attenuated, the mediating effect of organizational cynicism on the relationship between abusive supervision and turnover intention. Underlying mechanisms and implications, as well as avenues for future research, are discussed.

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Pitacho, Liliana, Patrícia Jardim da Palma, Pedro Correia, and Miguel Pereira Lopes. "Why Do People Work? An Empirical Test of Hybrid Work Orientations." Social Sciences 10, no. 8 (July 26, 2021): 284. http://dx.doi.org/10.3390/socsci10080284.

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The present study analyzed whether one’s work orientation can be organized into work orientation profiles beyond the three pure orientations of job, career, and calling. We tested the existence of these hybrid work orientations in a sample of 959 adults aged from 18 to 71 years old (M = 40.61, SD = 9.54). A cluster analysis showed that the best result consisted of four profiles: “Career-Calling”, “Career-Job”, “Pure Job”, and “Indifference”. Theoretical and practical implications of profile approach to the study of work orientations are discussed at the end.

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Smith, Lucian P., Jon A. Yamato, and Mary K. Kuhner. "CNValidator: validating somatic copy-number inference." Bioinformatics 35, no. 15 (December 12, 2018): 2660–62. http://dx.doi.org/10.1093/bioinformatics/bty1022.

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Abstract Motivation CNValidator assesses the quality of somatic copy-number calls based on coherency of haplotypes across multiple samples from the same individual. It is applicable to any copy-number calling algorithm, which makes calls independently for each sample. This test is useful in assessing the accuracy of copy-number calls, as well as choosing among alternative copy-number algorithms or tuning parameter values. Results On a dataset of somatic samples from individuals with Barrett’s Esophagus, CNValidator provided feedback on the correctness of sample ploidy calls and also detected data quality issues. Availability and implementation CNValidator is available on GitHub at https://github.com/kuhnerlab/CNValidator. Supplementary information Supplementary data are available at Bioinformatics online.

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John, Aji, Kathleen Muenzen, and Kristiina Ausmees. "Evaluation of serverless computing for scalable execution of a joint variant calling workflow." PLOS ONE 16, no. 7 (July 9, 2021): e0254363. http://dx.doi.org/10.1371/journal.pone.0254363.

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Advances in whole-genome sequencing have greatly reduced the cost and time of obtaining raw genetic information, but the computational requirements of analysis remain a challenge. Serverless computing has emerged as an alternative to using dedicated compute resources, but its utility has not been widely evaluated for standardized genomic workflows. In this study, we define and execute a best-practice joint variant calling workflow using the SWEEP workflow management system. We present an analysis of performance and scalability, and discuss the utility of the serverless paradigm for executing workflows in the field of genomics research. The GATK best-practice short germline joint variant calling pipeline was implemented as a SWEEP workflow comprising 18 tasks. The workflow was executed on Illumina paired-end read samples from the European and African super populations of the 1000 Genomes project phase III. Cost and runtime increased linearly with increasing sample size, although runtime was driven primarily by a single task for larger problem sizes. Execution took a minimum of around 3 hours for 2 samples, up to nearly 13 hours for 62 samples, with costs ranging from $2 to $70.

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Kim, Chang Sik, Sumitra Mohan, Mahmood Ayub, Dominic G. Rothwell, Caroline Dive, Ged Brady, and Crispin Miller. "In silico error correction improves cfDNA mutation calling." Bioinformatics 35, no. 14 (December 6, 2018): 2380–85. http://dx.doi.org/10.1093/bioinformatics/bty1004.

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Abstract Motivation Circulating-free DNA (cfDNA) profiling by sequencing is an important minimally invasive protocol for monitoring the mutation profile of solid tumours in cancer patients. Since the concentration of available cfDNA is limited, sample library generation relies on multiple rounds of PCR amplification, during which the accumulation of errors results in reduced sensitivity and lower accuracy. Results We present PCR Error Correction (PEC), an algorithm to identify and correct errors in short read sequencing data. It exploits the redundancy that arises from multiple rounds of PCR amplification. PEC is particularly well suited to applications such as single-cell sequencing and circulating tumour DNA (ctDNA) analysis, in which many cycles of PCR are used to generate sufficient DNA for sequencing from small amounts of starting material. When applied to ctDNA analysis, PEC significantly improves mutation calling accuracy, achieving similar levels of performance to more complex strategies that require additional protocol steps and access to calibration DNA datasets. Availability and implementation PEC is available under the GPL-v3 Open Source licence, and is freely available from: https://github.com/CRUKMI-ComputationalBiology/PCR_Error_Correction.git. Supplementary information Supplementary data are available at Bioinformatics online.

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Septianingsih, Selly, and Olivia Fachrunnisa. "The Meaning of Work and Career as Calling: A Model to Determine Millennial's Career Satisfaction." Jurnal Intelek 16, no. 2 (July 31, 2021): 206–17. http://dx.doi.org/10.24191/ji.v16i2.408.

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This research aims to examine and analyze the role of the meaning of work and career as a calling to determine career satisfaction in the millennial workforce. A career as a calling is an opportunity to create social work in the work environment. It is correlated to life satisfaction and meaning, career decisions, self-identity, and positive effectiveness. Meanwhile, the meaning of work for millennials is about the meaning of life. The millennial generation prefers to get freedom according to their characteristics and passions. Job satisfaction lies in self-actualization at work that does not only seek financial stability. The research was conducted using quantitative methods, data were collected from questionnaire and analyzed using partial least square (PLS). The sample population of this study comprised 150 millennial lecturers of Islamic universities in Semarang Indonesia. The criteria were those aged 20-40 years old with a minimum working period of one year. The results showed that digital fluency and spiritual work value have a positive and significant effect on the meaning of work and career as a calling. Meanwhile, the meaning of work and career as a calling has a positive and significant effect on career satisfaction.

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Palmer, Lance E., Mitchell J. Weiss, and Vikram R. Paralkar. "YODEL: Peak calling software for HITS-CLIP data." F1000Research 6 (July 18, 2017): 1138. http://dx.doi.org/10.12688/f1000research.11861.1.

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YODEL is a peak calling software for analyzing RNA sequencing data generated by High-Throughput Sequencing of RNA isolated by Crosslinking Immunoprecipitation (HITS-CLIP; also known as CLIP-SEQ), a method to identify RNA-protein interactions genome-wide. We designed YODEL to analyze HITS-CLIP experiments, in which Argonaute proteins are immunoprecipitated, followed by sequencing of the associated RNA in order to identify bound microRNAs and their mRNA targets. The HITS-CLIP sequenced reads are mapped to the genome, and then read peaks are visualized where clustered sets of reads map to the same region. Several peak calling algorithms have been developed to define the boundaries of these peaks. In contrast to other peak callers for HITS-CLIP data, such as Piranha, YODEL does not map the starts of reads to fixed interval bins, but instead uses a heuristic approach to iteratively find the tallest point within a set clustered reads and examine bases upstream and downstream of that point until a peak has been determined. This allows the peak boundary to be defined more precisely than coordinates that are multiples of the bin size. Per-sample peak counts are also generated by YODEL, which quickly enables downstream differential representation analysis. YODEL is available athttps://github.com/LancePalmerStJude/YODEL/.

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Ryan, Allison, Nathan Hunkapiller, Milena Banjevic, Naresh Vankayalapati, Nicole Fong, Kristine N. Jinnett, Zachary Demko, et al. "Validation of an Enhanced Version of a Single-Nucleotide Polymorphism-Based Noninvasive Prenatal Test for Detection of Fetal Aneuploidies." Fetal Diagnosis and Therapy 40, no. 3 (2016): 219–23. http://dx.doi.org/10.1159/000442931.

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Objective: To validate an updated version (Version 2) of a single-nucleotide polymorphism (SNP)-based noninvasive prenatal test (NIPT) and to determine the likelihood of success when testing for fetal aneuploidies following a redraw. Methods: Version 2 was analytically validated using 587 plasma samples with known genotype (184 trisomy 21, 37 trisomy 18, 15 trisomy 13, 9 monosomy X, 4 triploidy and 338 euploid). Sensitivity, specificity and no-call rate were calculated, and a fetal-fraction adjustment was applied to enable projection of these values in a commercial distribution. Likelihood of success of a second blood draw was computed based on fetal fraction and maternal weight from the first draw. Results: Validation of this methodology yielded high sensitivities (≥99.4%) and specificities (100%) for all conditions tested with an observed no-call rate of 2.3%. The no-call threshold for sample calling was reduced to 2.8% fetal fraction. The redraw success rate was driven by higher initial fetal fractions and lower maternal weights, with the fetal fraction being the more significant variable. Conclusions: The enhanced version of this SNP-based NIPT method showed a reduced no-call rate and a reduced fetal-fraction threshold for sample calling in comparison to the earlier version, while maintaining high sensitivity and specificity.

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Ip, Eddie K. K., Clinton Hadinata, Joshua W. K. Ho, and Eleni Giannoulatou. "dv-trio: a family-based variant calling pipeline using DeepVariant." Bioinformatics 36, no. 11 (April 21, 2020): 3549–51. http://dx.doi.org/10.1093/bioinformatics/btaa116.

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Abstract Motivation In 2018, Google published an innovative variant caller, DeepVariant, which converts pileups of sequence reads into images and uses a deep neural network to identify single-nucleotide variants and small insertion/deletions from next-generation sequencing data. This approach outperforms existing state-of-the-art tools. However, DeepVariant was designed to call variants within a single sample. In disease sequencing studies, the ability to examine a family trio (father-mother-affected child) provides greater power for disease mutation discovery. Results To further improve DeepVariant’s variant calling accuracy in family-based sequencing studies, we have developed a family-based variant calling pipeline, dv-trio, which incorporates the trio information from the Mendelian genetic model into variant calling based on DeepVariant. Availability and implementation dv-trio is available via an open source BSD3 license at GitHub (https://github.com/VCCRI/dv-trio/). Contact e.giannoulatou@victorchang.edu.au Supplementary information Supplementary data are available at Bioinformatics online.

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Sethi, Suresh A., Daniel Linden, John Wenburg, Cara Lewis, Patrick Lemons, Angela Fuller, and Matthew P. Hare. "Accurate recapture identification for genetic mark–recapture studies with error-tolerant likelihood-based match calling and sample clustering." Royal Society Open Science 3, no. 12 (December 2016): 160457. http://dx.doi.org/10.1098/rsos.160457.

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Error-tolerant likelihood-based match calling presents a promising technique to accurately identify recapture events in genetic mark–recapture studies by combining probabilities of latent genotypes and probabilities of observed genotypes, which may contain genotyping errors. Combined with clustering algorithms to group samples into sets of recaptures based upon pairwise match calls, these tools can be used to reconstruct accurate capture histories for mark–recapture modelling. Here, we assess the performance of a recently introduced error-tolerant likelihood-based match-calling model and sample clustering algorithm for genetic mark–recapture studies. We assessed both biallelic (i.e. single nucleotide polymorphisms; SNP) and multiallelic (i.e. microsatellite; MSAT) markers using a combination of simulation analyses and case study data on Pacific walrus ( Odobenus rosmarus divergens ) and fishers ( Pekania pennanti ). A novel two-stage clustering approach is demonstrated for genetic mark–recapture applications. First, repeat captures within a sampling occasion are identified. Subsequently, recaptures across sampling occasions are identified. The likelihood-based matching protocol performed well in simulation trials, demonstrating utility for use in a wide range of genetic mark–recapture studies. Moderately sized SNP (64+) and MSAT (10–15) panels produced accurate match calls for recaptures and accurate non-match calls for samples from closely related individuals in the face of low to moderate genotyping error. Furthermore, matching performance remained stable or increased as the number of genetic markers increased, genotyping error notwithstanding.

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Duan, Junbo, Han Liu, Lanling Zhao, Xiguo Yuan, Yu-Ping Wang, and Mingxi Wan. "Detection of False-Positive Deletions from the Database of Genomic Variants." BioMed Research International 2019 (April 4, 2019): 1–8. http://dx.doi.org/10.1155/2019/8420547.

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Next generation sequencing is an emerging technology that has been widely used in the detection of genomic variants. However, since its depth of coverage, a main signature used for variant calling, is affected greatly by biases such as GC content and mappability, some callings are false positives. In this study, we utilized paired-end read mapping, another signature that is not affected by the aforementioned biases, to detect false-positive deletions in the database of genomic variants. We first identified 1923 suspicious variants that may be false positives and then conducted validation studies on each suspicious variant, which detected 583 false-positive deletions. Finally we analysed the distribution of these false positives by chromosome, sample, and size. Hopefully, incorrect documentation and annotations in downstream studies can be avoided by correcting these false positives in public repositories.

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Esse, Ruben. "ChIPdig: a comprehensive user-friendly tool for mining multi-sample ChIP-seq data." F1000Research 8 (July 31, 2019): 1295. http://dx.doi.org/10.12688/f1000research.20027.1.

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In recent years, epigenetic research has enjoyed explosive growth as high-throughput sequencing technologies become more accessible and affordable. However, this advancement has not been matched with similar progress in data analysis capabilities from the perspective of experimental biologists not versed in bioinformatic languages. For instance, chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) is at present widely used to identify genomic loci of transcription factor binding and histone modifications. Basic ChIP-seq data analysis, including read mapping and peak calling, can be accomplished through several well-established tools, but more sophisticated analyzes aimed at comparing data derived from different conditions or experimental designs constitute a significant bottleneck. We reason that the implementation of a single comprehensive ChIP-seq analysis pipeline could be beneficial for many experimental (wet lab) researchers who would like to generate genomic data. Here we present ChIPdig, a stand-alone application with adjustable parameters designed to allow researchers to perform several analyzes, namely read mapping to a reference genome, peak calling, annotation of regions based on reference coordinates (e.g. transcription start and termination sites, exons, introns, and 5' and 3' untranslated regions), and generation of heatmaps and metaplots for visualizing coverage. Importantly, ChIPdig accepts multiple ChIP-seq datasets as input, allowing genome-wide differential enrichment analysis in regions of interest to be performed. ChIPdig is written in R and enables access to several existing and highly utilized packages through a simple user interface powered by the Shiny package. Here, we illustrate the utility and user-friendly features of ChIPdig by analyzing H3K36me3 and H3K4me3 ChIP-seq profiles generated by the modENCODE project as an example. ChIPdig offers a comprehensive and user-friendly pipeline for analysis of multiple sets of ChIP-seq data by both experimental and computational researchers. It is open source and available at https://github.com/rmesse/ChIPdig.

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Xu, Hui, and Terence J. G. Tracey. "Career Decision Ambiguity Tolerance and Its Relations With Adherence to the RIASEC Structure and Calling." Journal of Career Assessment 25, no. 4 (August 29, 2016): 715–30. http://dx.doi.org/10.1177/1069072716665874.

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While career decision ambiguity tolerance (CDAT) has been repeatedly shown to play a salient role in career decision-making, a potential revision of its measurement model has been indicated in past research. More importantly, it remains less clear as to what individual characteristics are associated with high CDAT. We examined a four-factor model of CDAT (i.e., preference, tolerance, confidence, and aversion) and its associations with adherence to the realistic, investigative, artistic, social, enterprise, and conventional (RIASEC) structure and career calling in a sample of college students ( N = 248). The results of the current study supported the four-factor model being superior to the original three-factor model in representing the structure of CDAT. It was also found that adherence to the RIASEC structure was positively associated with preference, presence of calling was positively associated with confidence, and search for calling was positively associated with aversion. The implications of the results were discussed with respect to the theory and research of CDAT. Limitations and suggestions for future research were also provided.

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Erum, Humaira, Ghulam Abid, and Francoise Contreras. "THE CALLING OF EMPLOYEES AND WORK ENGAGEMENT: THE ROLE OF FLOURISHING AT WORK." Business, Management and Education 18, no. 1 (February 24, 2020): 1–13. http://dx.doi.org/10.3846/bme.2020.11430.

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Purpose – Both the researchers and practitioners believe that engaged, energetic and focused employees provide sustainable competitive advantage to the organization. Therefore, the aim of this study was to explore whether the calling of the employees is related to work engagement and to observe if flourishing at work exerts a mediating role in this proposed relationship. Research Methodology – The time-lag method was followed to collect data from a sample of 101 employees working in different organizations from service industry. Findings – Results showed that calling is a critical psychological driver of work engagement explaining about 30% variance and it is valuable in shaping the employees orientation. Limitations – The data collection for the study was restricted to one major city so care must be taken in generalizing the results. Moreover, the use of cross sectional data may not completely capture the true nature of the psychological constructs like calling, flourishing and engagement. Implications – This study helps human resources managers to hire individuals who feel “called” to the job and devise training programs that shape their work orientation in order to engage and retain them. Originality – The current study considered work engagement as a psychological state and empirically tested psychological drivers- calling as the work orientation and flourishing. The proposed relationships, to the best of our knowledge, were not empirically tested previously.

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Amato, Robert J., Kevin P. Rosenblatt, Mehdi Dehghani, Glauco R. Souza, Reynolds Brobey, Cristian Ionescu-Zanetti, Jeff Jensen, and Hubert Tseng. "Isolation and analysis of circulating tumor cell samples from both spiked analytical sources and patients with renal cell cancer and prostate cancer." Journal of Clinical Oncology 34, no. 2_suppl (January 10, 2016): 584. http://dx.doi.org/10.1200/jco.2016.34.2_suppl.584.

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584 Background: Tumor genotyping using fluid samples such as blood can potentially allow tracking of dynamic changes in mutational profiles over time and allow better access than biopsies. We present a method to detect somatic mutations from a blood draw, where circulating tumor cell (CTC) enrichment above 10% of total cell numbers allows the use of standard gene panels typically used to analyze tissue-based biopsies. Methods: Analytical samples were obtained from 9 prostate cancer (PC) patients and 6 renal cell cancer (RCC) patients, followed by CTC enrichment using the IsoFlux System. Cells were lysed and DNA amplified by whole genome amplification (WGA) using the NGS Kit (Fluxion Biosciences) and quantified via qPCR. Samples were enumerated to determine CTC load, with CTCs defined as CK+, CD45- nucleated cells (DAPI+). Next-generation sequencing was performed using 3 targeted cancer panels on the Ion torrent PGM platform: the Ion ampliseq cancer hotspot panel (50 genes; 6 PC samples), the Oncomine (143 genes; 3 PC samples), and a 29-gene panel of actionable mutations in RCC (6 samples). Data was analyzed using a customized variant calling/filtering pipeline based on standard alignment and variant calling tools. Variant filtering and functional interpretation was performed using VarSeq. All data was analyzed in a blinded manner. Results: Our method was able to isolate CTCs from all patient samples. Whole genome amplified DNA concentration was at a range of 25-164 ng/µL (median, 69) in PC and 29-180 ng/µL (median, 69) in RCC. CTCs were recovered in 2.9-33.7% (median, 10.5%) of PC samples and 1.9-33% (median, 14.5%) of RCC samples After WGA, we found 1 variant/sample using hotpot, 12/sample using Oncomine, and 3/sample using the RCC panel. Conclusions: Our assay consistently detected somatic variants from blood draw using standard gene panels in both PC and RCC. Obtaining repeat tumor biopsies from patients during treatment and/or at time of progression is both challenging and impractical from a clinical perspective. Our assay provides molecular characterization using standard blood draws and will be prospectively validated in clinical trials.

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Journal articles on the topic 'Sample calling'

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