Academic literature on the topic 'San Germán (P.R.)'

Abstract Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = < 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P < 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.

It has been hypothesized that high non-physiological IGF-1 levels are partially responsible for the recurrent pregnancy loss observed in women with the polycystic ovary syndrome (Eng GS et al. 2007 Diabetes 56, 2228–2234). The aim of this study was to determine the effect of supraphysiological concentrations of IGF-1 on blastocyst production and the occurrence of apoptosis in bovine embryos, which are a good model for human embryo development (Baumann CG et al. 2007 Mol. Reprod. Dev. 74, 1345–1353). COC obtained by slicing from abattoir ovaries were matured (TCM-199, Sigma) for 24 h and fertilized (Fert-TALP) for 18 h (Day 0) in vitro. Two different IGF-1 (Recombinant human IGF-1, R&D Systems GmbH, Wiesbaden, Germany) concentrations (supraphysiological = 1000 ng mL–1 and physiological = 100 ng mL–1) were added to the culture media (Synthetic oviduct fluid/BSA) and compared with a control group (no IGF-1 supplementation). On Day 8, blastocyst rates (22 replicates) were recorded and DNA degradation was detected in blastocyst nuclei using a cell death detection kit (Roche Diagnostics GmbH, Mannheim, Germany) based on the terminal deoxinucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) principle. Embryos (n = 27 [control], n = 29 [both IGF-1 groups]) from 4 replicates were examined by confocal laser scanning microscopy. Data were analyzed by ANOVA and the Fisher exact test using the SigmaStat 2.0 software package (Jandel Scientific, San Rafael, CA). Cleavage was numerically improved by both, 1000 (59.1 ± 1.8) and 100 (58.2 ± 2.8) ng IGF-1 over controls (53.5 ± 2.2), but the differences did not reach statistical significance (P = 0.22). The proportion of hatched blastocysts was enhanced by 100 (5.8 ± 1.0, P = 0.03) and 1000 (5.1 ± 0.7, P = 0.03) ng IGF-1 compared to controls (2.8 ± 0.6). Total blastocyst rate was increased by 100 ng IGF-1 (34.4 ± 1.9, P = 0.02) over controls (28.3 ± 1.7), but not by 1000 ng IGF-1 (29.1 ± 1.6 P = 0.75). The 100 ng IGF-1 group (38.5 ± 3.7) had fewer degenerated embryos (P = 0.01) compared to 1000 ng IGF-1 (49.7 ± 3.3). The proportion of embryos displaying at least one apoptotic cell was greater in the 1000 ng IGF-1 group over controls (96% v. 77% P = 0.04). The number of blastomeres with TUNEL-positive nuclei per embryo was higher in the supraphysiological group (5.5 ± 0.6, P < 0.001) compared with the control (2.3 ± 0.4) and the physiological group (2.5 ± 0.3). There were no significant differences between the control and the 100 ng IGF-1 group in this regard (P = 0.49). In conclusion, supraphysiological concentrations of IGF-1 do not increase blastocyst production but increase levels of apoptosis in bovine embryos produced in vitro. M. A. V. is in the PhD program of the University of Veterinary medicine, Hannover, Germany, and is supported by the German Academic Exchange Service (DAAD)

Abstract Abstract 4487 HLA mismatch constitute the risk of aGvHD. However, environmental factors play a significant role what is exemplified by the protective effect of germ free environment. This association can be discussed on the ground of a significant role of cytokine milieu which determines lymphocytes subsets differentiation. Our previous work showed a decrease of proportions of Th17 cells in blood at the onset of aGvHD (Dlubek et al., Transplantation Proceedings 2010; 42(8):3277-9). These cells, however, were seen at the tissue site likely being marginalized during aGvHD process. In this study we looked at Th17 lymphocytes proportions in blood of patients post alloHSCT in the context of an increase of C-reactive protein in serum and NOD2/CARD15 gene mutations. Eighty four patients (median age: 41 yrs, range: 1–64 yrs) entered the study. All of them were clinically followed for the presence of infections complications and aGvHD. Blood work was done at the very first day of the later complications and in addition routinely in one week intervals beginning from the day of transplantation. The cells were labeled for CD4 (Becton Dickinson, San Jose, CA, USA), intracellular IL-17A (e-biosciences, San Diego, CA, U04)SA) and FoxP3 (Becton Dickinson, San Jose, CA, USA) detection. CRP was measured in serum using a Behring BN Prospect nephelometer (Dade Behring Inc., Marburg, Germany). All studied cases were genotyped for NOD2/CARD15 mutations: 104C-T (SNP8, Arg702W; rs.2066844), 2722G-C (SNP12, Gly908Arg; rs.2066845), and 3020insC (SNP13, Leu1007fsinsC; rs.2066847) with the use of RFLP-PCR technique. It was found: Acute GvHD manifestation was seen in 34 (40%) patients among them 22 (26%) had only skin symptoms and 12 (14%) presented gut manifestation. Gut aGvHD was seen at later time post HSCT than skin aGvHD (median 33 vs. 21 days, M-W U test p=0.035).Twelve patients had NOD2/CARD15 mutations. They had lower proportions of Th17 lymphocytes in blood as compared to those lacking these mutations (median 0.03 vs. 0.07%, M-W U test p=0.052) and 6 (50%) of them presented aGvHD.CRP levels measured from 3 to 5 days before clinically apparent aGvHD were significantly higher in patients with gut symptoms as compared to those presented only skin lesions (median 66.8 vs. 7.61 mg/L, M-W U test p=0.020). Notably, patients having only skin symptoms had rather lower CRP levels in sera than those without aGvHD (median 7.61 vs. 27.6 mg/L, M-W U test p=0.028).In patients with gut manifestation FoxP3+CD4+ cells proportions were negatively correlated with serum CRP levels (Spearman r −0.664, p=0.018) what was not seen in patients presented only skin symptoms aGvHD (0.068, p=0.769). In conclusion: CRP elevation constituted the risk of gut aGvHD and was associated with rather exhausted T regulatory cell function. NOD2/CARD15 mutations associated factors contributed to the lowering of Th17 cells in blood what was previously found to be associated with the overt manifestation of aGvHD (Dlubek et al., Transplantation Proceedings 2010; 42(8):3277-9). These both findings are in line with the hypothetical role of microbial pathogens which via inflammation favor differentiation of lymphocytes to Th17 positive cell subset. Disclosures: No relevant conflicts of interest to declare.

MacWhinney, Bates, and colleagues developed the Competition Model in the 1980s as an alternate to Chomskyan models that encapsulate syntax as a special-purpose module. The Competition Model adopted the functional perspective that language serves communicative goals and functions. In contrast to the premise that knowledge of language is innate, the Competition model asserts that language is learned and processed through general cognitive mechanisms that identify and weight phonological, morphological, syntactic, and semantic cues in the language experiences of the learner. These weighted cues guide the language user in the comprehension and production of language forms. The present article provides background on the Competition Model, describes machine simulations of linguistic competition, and extends the principles of the Competition Model to new machine models and applications through deep learning networks. References Bates, E. & MacWhinney, B. (1982). A functionalist approach to grammar. In E. Wanner & L. Gleitman (Eds.), Language acquisition: the state of the art. New York: Cambridge University Press. Bates, E., & MacWhinney, B. (1989). Functionalism and the competition model. In: The Crosslinguistic Study of Sentence Processing, (pp 3-76). B. MacWhinney and E. Bates (Eds.), New York: Cambridge University Press. Devescovi, A., D’Amico, S., Smith, S., Mimica, I., & Bates, E. (1998). The development of sentence comprehension in Italian and Serbo-Croatian: Local versus distributed cues. In: Syntax and Semantics: Vol. 31. Sentence Pocessing: A Cross-Linguistic Perspective, (pp. 345-377). D. Hillert (Ed.), San Diego: Academic Press. Hauser, M. D., Chomsky, N., & Fitch, W. T. (2002). The faculty of language: What it is, who has it, and how did it evolve? Science, 298, 1569-1579. Just, M. A., & Carpenter, P. A. (1980). A theory of reading: From eye fixations to comprehension. Psychological Review, 87, 329-354. Langacker, R. (1989). Foundations of cognitive grammar. Vol. 2: Applications. Stanford: Stanford University Press. Li, P., & MacWhinney, B. (2013). Competition model. In: The Encyclopedia of Applied Linguistics. C. A. Chapelle (Ed.), Malden, MA: Wiley. MacWhinney, B. (1987). The competition model. In: Mechanisms of Language Acquisition, (pp.249-308). B. MacWhinney (Ed.).Hillsdale, NJ: Lawrence Erlbaum. MacWhinney, B. (2001). The competition model: The input, the context, and the brain. In: Cognition and Second Language Instruction, (pp. 69–90). P. Robinson (Ed.), New York: Cambridge University Press. MacWhinney, B. (2008). A Unified Model. In: Handbook of Cognitive Linguistics and Second Language Acquisition, (pp. 341-371). P. Robinson & N. Ellis (Eds.). Mahwah, NJ: Lawrence Erlbaum Associates. MacWhinney B. (2012). The logic of the Unified Model. In: The Routledge Handbook of Second Language Acquisition, (pp. 211–227). S. Gass and A. Mackey (Eds.). New York: Routledge. MacWhinney, B. (2015). Multidimensional SLA. In: Usage-Based Perspectives on Second Language Learning, (pp. 22-45). S. Eskilde and T. Cadierno (Eds.). New York: Oxford University Press. MacWhinney, B., Bates, E. & Kliegl, R. (1984). Cue validity and sentence interpretation in English, German, and Italian. Journal of Verbal Learning and Verbal Behavior, 23, 127-150. MacWhinney, B., Leinbach, J., Taraban, R., & McDonald, J. (1989). Language learning: Cues or rules? Journal of Memory and Language, 28, 255-277. McClelland, J. L., & Rumelhart, D. E. (1986). Parallel Distributed Processing. Explorations in the Microstructure of Cognition. Volume 2: Psychological and Biological Models. Cambridge, MA: MIT Press. Presson, N. & MacWhinney, B. (2011). The Competition Model and language disorders. In: Handbook of Psycholinguistic and Cognitive Processes, (pp. 31-48). J. Guendozi, F. Loncke, and M. Williams (Eds.). New York: Psychology Press. Sokolov, J. L. (1988). Cue validity in Hebrew sentence comprehension. Journal of Child Language, 15, 129-156. Taraban, R. (2004). Drawing learners’ attention to syntactic context aids gender-like category induction. Journal of Memory and Language, 51(2), 202-216. Taraban, R. (2017). Hate, white supremacy, PTSD, and metacognition. In: Improve With Metacognition [online]. L. Scharff, A. Richmond, & J. Draeger (Eds.). Retrieved from: www.improvewithmetacognition.com. Taraban, R., & Kempe, V. (1999). Gender processing in native and non-native Russian speakers. Applied Psycholinguistics, 20, 119-148. Taraban, R., McDonald, J., & MacWhinney, B. (1989). Category learning in a connectionist model: Learning to decline the German definite article. In R. Corrigan, F. Eckman, & M. Noonan (Eds.), Linguistic categorization (pp. 163-193). Philadelphia: Benjamins. Taraban, R., & Roark, B. (1996). Competition in learning language-based categories. Applied Psycholinguistics, 17, 125-148.

Glucocorticoids are mediators of the systemic stress response. Acute or chronic stress characterised by high cortisol concentrations in the periphery impairs reproductive function in a variety of species and therefore may affect fertility. The ovary has been shown to be a target tissue for glucocorticoids in many species, including the mare. This study hypothesised that the equine ovary possesses internal mechanisms to modulate cortisol activity and that supraphysiologic levels of glucocorticoids do not affect oocyte IVM rates. Light horse mares (n = 9) were used in this study. Growing follicles from an induced follicular wave were divided into the following groups: G1: 5–9 mm, G2: 10–14 mm, G3: 15–19 mm, G4: 20–24 mm, and G5: ≥25 mm. Follicular fluid (FF) and compact cumulus‐oocyte complexes (COCs) were obtained by ultrasound-guided transvaginal aspiration. Blood samples were collected at the beginning and the end of every aspiration session. Cortisol (DE1887, Demeditec, Kiel-Wellsee, Germany), progesterone (ADI-901–011, Enzo Life Sciences, Farmingdale, NY, USA), and corticosteroid binding globulin (CBG, MBS047353, MyBioSource, San Diego, CA, USA) concentrations were determined by ELISA. COCs (n = 80) were randomly distributed to either the control group (DMEM-F12+ medium) or the following hydrocortisone treatment groups: 0.1 µg mL–1, 1 µg mL–1, 5 µg mL–1, 10 µg mL–1. Maturation rate was assessed 30 h after incubation. Statistical analysis was performed with the SPSS Statistics 22 software. Data were analysed using one-way ANOVA, Pearson correlation, and chi-squared test. Cortisol (115.4 ± 13.3 ng mL–1) and progesterone (22.1 ± 3.1 ng mL–1) FF concentrations were higher (P < 0.05) in G5 follicles than in all other groups, and were positively correlated (r = 0.8; P < 0.001). Plasma concentrations of cortisol (118.6 ± 7.8 v. 120.3 ± 12.2 ng mL–1), progesterone (2.4 ± 0.5 v. 2.5 ± 0.4 ng mL–1), and CBG (11.1 ± 5.1 v. 9.9 ± 3.2 µg mL–1) did not differ before and after follicle aspiration. However, plasma CBG and progesterone were negatively correlated (r = –0.56; P < 0.01). Maturation rates did not differ among groups, regardless of the hydrocortisone concentration added to the culture medium. Our results demonstrated higher cortisol concentrations in preovulatory follicles in vivo, suggesting its importance for oocyte maturation. The greater unbound cortisol available in the FF of preovulatory follicles can be indicative of the displacement of cortisol from CBG in favour of progesterone. Furthermore, equine oocytes were capable of surviving cortisol concentrations 100 times higher than those physiologically present in preovulatory follicles. This finding suggests the ability of equine oocytes to modulate cortisol levels and adapt to stress situations.

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Introduction. The purpose of the paper is to justify the possibility of developing ontology of augmented reality as a special sphere of social space. A comparative analysis of approaches to the interpretation of reality in philosophy and theoretical sociology is carried out. The original provisions of the conceptual model of social reality and social actuality have been formulated. The concepts of actual and virtual social actions are introduced and analyzed.Methodology and sources. The main provisions of phenomenological sociology are used as the methodological basis of the study: A. Schutz's interpretation of social reality as a fragment of being translated into the world of intersubjective, and P. Berger and T. Luckmann's characterization of the reality of society as a process of its construction in practical activities. The features of augmented reality, revealed in the works of R. Azuma, P. Milgram, A. F. Kishino, H. Papagiannis, A. M. Larsen, S. A. Glazkova, O. N. Kislova and other researchers, are taken into account. Descriptions of the characteristics of augmented reality devices are derived from reports from research center heads: the descriptions of the characteristics of augmented reality devices are derived from reports from the heads of research centers involved in the development and implementation of digital technologies: universities in London, Tokio and Toronto, Hasso-Plattner Institute in Germany, Finnish company Senseg, company Disney Research, company High Fidelity, MIT self-assembly labs, MIT Media Lab Group, San Francisco-based Detour startup, Google and Microsoft etc.Results and discussion. The environment shaped by augmented reality is much more complex than it is in its immediate perception. It includes four spaces: 1) subject world, 2) the mental world and 3) the hybrid world as a symbiosis of real and imaginary worlds, or 4) symbiosis of real-world fragments – torn in space and time and combined with technology in devices that give the individual's ability to be present when observing their combined existence.Conclusion. Augmented reality complicates virtual reality, adding to its content in addition to fictional characteristics the content of practical actions. Augmented reality, using the virtual reality resource, becomes reality as the basis of practice. Augmented reality not only “begets” the world, but is in direct practical contact with it, thus becoming a special side of social reality.

ABSTRACT Purpose To evaluate the sensitivity and specificity of the Pentacam topometric indices derived from the corneal surface curvature to distinguish between normal and keratoconic corneas. Methods The study consisted of 226 normal corneas from 113 patients and 88 keratoconic eyes from 44 patients. Eyes were defined as keratoconus based on comprehensive ocular examination, including Placido-disk-based corneal topography (Atlas Corneal Topography System; Humphrey, San Leandro, California) and rotating Scheimpflug corneal tomography (Pentacam HR; Oculus, Wetzlar, Germany). Corneal Topometric indices ISV, IVA, KI, CKI, IHA and IHD, along with the TKC (Topometric Keratoconus Classification) score were calculated from the Pentacam HR exam. Statistical analysis were accomplished using BioEstat 5.0 (Instituto Mamiraua, Amazonas, Brazil) and MedCalc 12.0 (MedCalc Software, Mariakerke, Belgium) using unpaired nonparametric Mann Whitney test (Wilcoxon ranked-sum). ROC curves were calculated for each topometric parameter to determine the best cut off values from the significantly different parameters. A logistic regression analysis was performed to provide a combined parameter for optimizing accuracy. Results Statistical significant differences were found between keratoconic and normal corneas for all topometric indices (Mann Whitney, p < 0.05). There were four false negative cases among the keratoconic cases on the TKC classification (4.54%) and 16 false positive cases among normal (7.08%), so that the sensitivity and specificity of the TKC were 95.54 and 92.92% respectively. The areas under the ROC curves (AUC) for the individual topometric indices varied from 0.843 (CKI) and 0.992 (ISV). The sensitivity and specificity of the most accurate ISV were 97.7 and 96.5% respectively. The calculated parameter from logistic regression had AUC of 0.996, with sensitivity of 97.7% and specificity of 98.7%. Conclusion Pentacam topometric indices were useful for distinguishing between normal and keratoconic corneas. The TKC classification should be expected to have false positives and negatives and should not be considered alone. TKC had more false positives and false negatives than some individual topometric parameters. A novel combined parameter based on logistic regression analysis may improve accuracy for the diagnosis of keratoconus. Further studies are necessary to evaluate if adding other curvature derived indices is beneficial for the regression analysis, as well as for testing the sensitivity of such parameters for the diagnosis of milder forms of ectasia and for testing correlations with severity of the disease. How to cite this article Salomao MQ, Guerra FP, Ramos IC, Jordao LF, Canedo ALC, Valbon BF, Luz A, Correa R, Lopes B, Ambrósio Jr R. Accuracy of Topometric Indices for Distinguishing between Keratoconic and Normal Corneas. J Kerat Ect Cor Dis 2013;2(3):108-112.

Background:Systemic Lupus Erythematosus (SLE) is an autoimmune disease that involves several molecular patterns with a wide spectrum of clinical manifestations and symptoms. Inflammation and related pathway play a role in SLE pathogenesis. The pentraxin superfamily including long and short pentraxin, C Reactive Protein CRP, Serum amyloid A (SAA), Pentraxin 3 (PTX3) are key components of innate immune system and induce a variety of inflammation associated pathway. However Literature provides several evidences that CRP serum levels not correlated with clinical and immunological manifestations. This situation affected clinical practice and the patient follow up. PTX3 have been identified as a component of inflammatory status in several autoimmune conditions. SAA is an acute phase protein secreted in large quantity during inflammation.Objectives:We want to evaluated SAA, PTX3 and CRP concentrations, their correlation between SLE Disease Activity Index (SLEDAI), that including complement fractions C3, C4.Methods:We enrolled fifty patients that fulfilled the SLE American College of Rheumatology criteria and fifty healthy subjects. The SLE disease activity was classified with the SLEDAI (0 to 12). Patients were divided into two groups according to SLEDAI score: inactive group (Group 1, 25 patients, 50%: SLEDAI < 4) and active group (Group 2, 25 patients, 50%: SLEDAI 5 to 12). PTX3 concentration was measured by a sandwich ELISA kit (Hycult) with 2.8 ng/mL cut-off point. SAA concentration was detected by nephelometry performed on a BN ProSpec System (Siemens, Germany), with assay kit based on polyclonal antibodies (Siemens Healthcare Diagnostics Products, Germany, 6.5 mg/L cut-off point). High sensitive CRP concentrations were determined using the ci8200 platform (Abbott Laboratories Chicago, Illinois).Results:Plasma PTX3 and serum SAA levels was significantly higher in SLE patients than in the healthy subjects (PTX311.5 ± 7.3 ng/mL vs 2.3 ± 1.1; p < 0.001; SAA: 87 ±77 mg/L vs 2.6±2.5; p < 0.001). These differences were not evident in CRP levels (8.5 ± 7.8 mg/L vs 6.2± 2.5). Considering two groups, there were statistical differences in PTX3 level (Group 2: 14.9 ± 12 ng/mL vs Group 1: 2.16 ±0.5 ng/mL, p<0,05) and SAA concentration (Group 2: 114 ± 89 ng/mL vs Group 1: 3.6 ±1.7 ng/mL, p<0,05) but not in CRP concentration (Group 2: 11.5 ± 8.4 mg/L vs Group 1: 9.5 ±3.5). There was a significantly negative correlation between C3, C4 fractions, PTX3 and SSA levels (respectively r = −0.74, p=<0.05, and r = −0.79, p<0.05). No statistical correlation were appeared between C3, C4 fractions and CRP serum levels (r= −0,12., p= 0.82, and r= −0.18, p= 0,21). We noted a positive significant correlation between SLEDAI, PTX3 and SAA concentration (r = 0.79, p < 0.05, 0.83, p < 0.05, respectively) an increase in PTX3 and SAA levels followed the lupus flare and symptoms. No significant correlation appeared between SLEDAI and CRP (r= 0.15, p=0.89)Conclusion:PTX3 and SAA concentration was significantly higher in SLE patients than the healthy control subjects and their levels reflected disease activity. We showed a direct correlation between PTX3 and SAA. In SLE patients PTX3 and SAA concentrations were correlated with SLEDAI. We suggest an integrate viewpoint in witch SAA and PTX3 may play a role as a biomarker of disease activity, with synergic work during SLE events. Evidences suggested that PTX3 and SAA could trigger the same molecular pathway, by TLR4, via NF-kB.References:[1]Assandri R, Monari M Montanelli A. Pentraxin 3 in Systemic Lupus Erithematosus: Questions to be Resolved, Translational Biomedicine (2015)Disclosure of Interests:None declared

Abstract Background We have previously reported on the high prevalence and severity of insomnia amongst a large international cohort of MPN patients (Emanuel JCO 2012). We sought to further analyze the relationships between insomnia and the MPN disease features (in particular splenomegaly, status of blood counts), individual MPN symptom prevalence and severity, language, and overall quality of life. Methods Data was collected among an international cohort of patients with MPNs. Subjects completed the BFI, MPN-SAF, and EORTC QLQ-C30 instruments. Surveyed symptoms on the MPN-SAF included the patient’s perceptions of common MPN-related symptoms and overall quality of life (QOL) on a 0 (absent) to 10 (worst imaginable) scale. Specifically, the MPN-SAF insomnia item asked about “difficulty sleeping”. Total symptom score (TSS) was computed based on 10 symptom items using the published scoring algorithm on a 0 (all reported symptoms absent) to 100 (all reported symptoms worst imaginable) scale. Pairwise associations between the MPN-SAF insomnia item and continuous and categorical covariates were investigated using Pearson correlations and analysis of variance/t-tests, respectively. Multivariate regression models were used to investigate impact of groups of covariates on the insomnia item with the final multivariate model selected using forward regression. Results Demographics A total of 1992 MPN patients (essential thrombocythemia=834, polycythemia vera=703, myelofibrosis=449, unknown=6) completed the insomnia item. Participants were of typical age (median=61, range-15-94) and gender (female=54%). Overall, 1307 subjects endorsed the MPN insomnia item (score >0) with an overall mean symptom score of 3.0 (median=2.0, SD=3.1. range=0-10); 603/1992 (30%) of patients had severe insomnia related complaints (score >4). Univariate Analysis Among QLQ-C30 functioning scales, emotional functioning most highly correlated with the MPN-SAF insomnia item (r=-.47, p<0.001) with all other domains also having statistically significant but slightly weaker correlation (all p<0.001). Among QLQ-C30 symptom scales, insomnia (r=.79, p<0.001) and fatigue (r=.45, p<0.001) most highly correlated with the MPN-SAF insomnia item with all other QLQ-C30 symptoms having correlations between .13 and .37 (all p<.001). Among MPN-SAF items, the MPN-SAF insomnia item correlated with all MPN-SAF items (all p<0.001) including the TSS (r=0.55, p<0.001) and most highly with depression (r=.52, p<0.001), concentration problems (r=.43, p<0.001), overall QOL (r=.41, p<0.001), night sweats (r=.41, p<0.001), and extremity tingling (r=.40, p<0.001). MPN-SAF insomnia significantly differed by gender (means: M 2.4, F 3.4; p<0.001), language (means ranged from 2.6 [Italian] to 3.6 [German]; p=0.01), anemia (means: absent 2.8, present 3.5; p<0.001), prior hemorrhage (means: no 2.9, yes 3.7; p=0.01), and requiring red blood cell transfusion (means: no 2.9, yes 3.6; p=0.03), with a trend for a difference by MPN type (ET mean=2.7, PV mean=3.0, MF mean=3.2; p=0.06). Multivariate Analysis In a multivariate model of MPN-SAF insomnia containing all QLQ-C30 functioning scales, physical, emotional, and cognitive functioning along with global health status/QOL were all significant (all p<0.001). Numerous symptoms were also significant in a multivariate model of MPN-SAF insomnia containing all other MPN-SAF symptoms (all p<0.05: headaches, extremity tingling, depression, sexual problems, night sweats, pruritus, fever, and QOL). The final multivariate model based on forward regression starting with all demographics, clinical variables, and patient-reported QLQ-C30 scales and MPN-SAF items found age, gender, QLQ-C30 emotional functioning and MPN-SAF items to be significant. Conclusions Insomnia is highly prevalent and severe in MPN patients and closely correlates with most other MPN-related symptoms and functional domains bearing a multi-faceted impact on overall quality of life. Correlations between insomnia and emotional, cognitive, and physical complaints including depression/sad mood, concentration problems, night sweats, and numbness/tingling in the extremities suggest that the cause of MPN-related sleep complaints is likely complex. Future studies should evaluate the impact of interventions on MPN associated insomnia as well as its biological underpinnings. Disclosures: Etienne: novartis: Consultancy, Membership on an entity’s Board of Directors or advisory committees; Bristol Myers Squibb: Consultancy, Membership on an entity’s Board of Directors or advisory committees; Pfizer: Membership on an entity’s Board of Directors or advisory committees; Ariad: Membership on an entity’s Board of Directors or advisory committees. Roy:Novartis, BMS: Honoraria. Harrison:Novartis: Honoraria, Membership on an entity’s Board of Directors or advisory committees, Research Funding, Speakers Bureau; Sanofi: Honoraria, Membership on an entity’s Board of Directors or advisory committees, Speakers Bureau; YM Bioscience: Honoraria, Membership on an entity’s Board of Directors or advisory committees; Celgene: Honoraria; Shire: Speakers Bureau; S Bio: Honoraria, Membership on an entity’s Board of Directors or advisory committees; Gilead: Honoraria, Membership on an entity’s Board of Directors or advisory committees. Birgegard:Vifor Pharma: Honoraria.

ANTECEDENTES: Se estima que mundialmente la prevalencia de malformaciones oscila entre 1.5 – 4%. En México y en América Latina se reporta dentro de las primeras causas de morbilidad y mortalidad infantil. OBJETIVO: Determinar la prevalencia de malformaciones congénitas de los neonatos que ingresaron al servicio de Neonatología en el periodo de enero 2009 a diciembre del 2010 del Hospital General Toluca del P.R. “Dr. Nicolás San Juan”. MATERIAL Y METODO: Se llevó a cabo un estudio retrospectivo, transversal, observacional y descriptivo. Se recabó información de los expedientes clínicos de pacientes ingresados al servicio de Neonatología con diagnóstico de malformación congénita. Se realizó análisis univariado a través de frecuencias simples para obtener la prevalencia por tipo de malformación congénita, género, edad gestacional, peso y factores maternos relacionados. RESULTADOS: En el periodo de estudio se registraron 10,595 nacimientos y 76 casos de malformación congénita con una prevalencia de 7.1/ 1000 nacidos. El síndrome de Down fue el prevalente con 1.3 por 1000 nacidos, y las malformaciones craneofaciales con 1.4/ 1000 nacidos, de éstas labio y paladar hendido con 1.1/ 1000 nacidos. Género prevalente fue el femenino, la mayoría de niños de término y eutróficos. Edad materna promedio 26.1 años, las malformaciones prevalecieron en mujeres multigestas, escolaridad primaria, y ocupación ama de casa. No se relacionaron toxicomanías. Diagnóstico prenatal de 6.5%. CONCLUSIONES: La prevalencia de malformaciones congénitas en nuestro estudio es inferior a la reportada en la literatura. Sin embargo se debe insistir en control prenatal adecuado para detección oportuna de malformaciones congénitas.

Maddalena Iovene¹, Graciela Fernandéz De Córdova2, Ombretta Romice¹, Sergio Porta¹ ¹Urban Design Studies Unit (UDSU). Department of Architecture. University of Strathclyde. 75 Montrose Street, Glasgow, G11XJ, UK. 2Centro de Investigación de la Arquitectura y la Ciudad (CIAC), Departamento de Arquitectura, PUCP. Av. Universitaria 1801, 32 San Miguel, Lima, Peru. E-mail: maddalena.iovene@strath.ac.uk, gdcfernandez@pucp.edu.pe, ombretta.r.romice@strath.ac.uk, sergioporta@strath.ac.uk Keywords (3-5): Informal Settlement, Peru, Lima, Model of Change, Urban Morphology Conference topics and scale: Reading and Regenerating the Informal City Cities are the largest complex adaptive system in human culture and have always been changing in time according to largely unplanned patterns of development. Though urban morphology has typically addressed studies of form in cities, with emphasis on historical cases, diachronic comparative studies are still relatively rare, especially those based on quantitative analysis. As a result, we are still far from laying the ground for a comprehensive understanding of the urban form’s model of change. However, developing such understanding is extremely relevant as the cross-scale interlink between the spatial and social-economic dynamics in cities are increasingly recognized to play a major role in the complex functioning of urban systems and quality of life. We study the urban form of San Pedro de Ate, an informal settlement in Lima, Peru, along its entire cycle of development over the last seventy years. Our study, conducted through a four-months on-site field research, is based on the idea that informal settlements would change according to patterns similar to those of pre-modern cities, though at a much faster pace of growth, yet giving the opportunity to observe the evolution of an urban organism in a limited time span. To do so we first digitalize aerial photographs of five different time periods (from 1944 to 2013), to then conduct a typo-morphological analysis at five scales: a) unit, b) building, c) plot, d) block, and e) settlement (comprehensive of public spaces and street network). We identify and classify patterns of change in the settlement’s urban structure using recognised literature on pre-modern cities, thus supporting our original hypothesis. We then suggest a unitary model of analysis that we name Temporal Settlement Matrix (TSM). Reference List Caniggia, G., &amp; Maffei, G. L. (2008). Lettura dell’edilizia di base (Vol. 215). Alinea Editrice. Conzen, M. R. G. (1958). The growth and character of Whitby. A Survey of Whitby and the Surrounding Area, 49–89. Hernández, F., Kellett, P. W., &amp; Allen, L. K. (2010). Rethinking the informal city: critical perspectives from Latin America (Vol. 11). Berghahn Books. Kropf, K. (2009). Aspects of urban form. Urban Morphology, 13(2), 105–120. Muratori, S. (1960). Studi per una operante storia urbana di Venezia. Palladio, 1959, 1–113. 22. Porta, S., Romice, O., Maxwell, J. A., Russell, P., &amp; Baird, D. (2014). Alterations in scale: patterns of change in main street networks across time and space. Urban Studies, 51(16), 3383–3400. Watson, V. (2009). “The planned city sweeps the poor away…”: Urban planning and 21st century urbanisation. Progress in Planning, 72(3), 151–193. Whitehand, J. W. R. (2001). Changing suburban landscapes at the microscale. Tijdschrift Voor Economische En Sociale Geografie, 92(2), 164–184.

Marinilla´s cultural landscape and spacial characterization (Colombia). Catalina Montoya Arenas¹, Lina María Escobar Ocampo¹, Claudia Maria Venegas Velez¹ ¹Facultad de Arquitectura, UPB. Circular 1 N°70-01 Medellin, Colombia. E-mail: catalina.montoyaarenas@upb.edu.co, lina.escobar@upb.edu.co, claudia.ve7@gmail.com Keywords (3-5): Cultural landscape, social management, heritage, spacial transformations, tourism Conference topics and scale: Stages in territorial configuration The historic center of Marinilla, National Monument since 1959, is located sixty minutes from Medellin at San Nicolas Valley. It has exceptional landscape conditions, highly productive lands, and a large percentage of the water reserve that supplies the region and the country, giving the territory an economic center character since the colony. These physical values make part of collective imagination as a recreation area and an opportunity for development in the 1960s, according to the construction of large national infrastructure works. At the same time, it was object of armed conflict in the 1980s and 1990s, and more recently, directly related to the spatial dynamics of the region: unplanned urban expansion, changes in land use and vegetation cover, with effects on the cultural landscape. In a post-conflict situation, the economic strategies of different actors trust on tourism as a social-spatial management strategy to improve the territory. However, the identity of rurality shows spatial imbalances without recognizing elements of historical construction whose legacies must be revealed to ensure equitable development. To do this, we propose an approach from the cultural landscape in a revision of the historical, symbolic and relational transformation through five systems (anthropic, productive, political, symbolic and spatial), analyzing competitiveness, tourism, landscape and social management, in different scales and during three historical moments. References (100 words) Busquets, J., and Cortina, A. (2009). Gestión del paisaje: Manual de protección, gestión y ordenación del paisaje. Ariel, Barcelona. Sierra, P. A. (2003). Periferias y nueva ciudad: el problema del paisaje en los procesos de dispersión urbana. Universidad de Sevilla. Barrera, S. (2014). Consideraciones teóricas para el análisis del paisaje. La Metodología de Los eventos relacionales. Perspectivas sobre el paisaje. Varón, D. C. Z. (2015). El derecho al paisaje en Colombia.: Consideraciones para la definición de su contenido, alcance y límites. U. Externado de Colombia. Olmo, R. M. (2008). El paisaje, patrimonio y recurso para el desarrollo territorial sostenible. Conocimiento y acción pública. Arbor, 184(729).