Relevant bibliographies by topics / Sanger sequencing

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Sanger sequencing'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2025

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Journal articles on the topic "Sanger sequencing"

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Estrada-Rivadeneyra, Diego. "Sanger sequencing." FEBS Journal 284, no. 24 (2017): 4174. http://dx.doi.org/10.1111/febs.14319.

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Dodson, Guy. "Fred Sanger: sequencing pioneer." Biochemist 27, no. 6 (2005): 31–35. http://dx.doi.org/10.1042/bio02706031.

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Fred Sanger is a biochemist who practised chemistry and whose discoveries have been fundamental to the development of modern biological science. His insights into sequencing methods and the central roles played by proteins and nucleic acids in the molecular processes of biology led him to dedicate a lifetime to their investigation. Together with his shrewd chemical judgement, his experimental skill and his tenacity, this made him one of the most productive and remarkable biochemists of our time.
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Carpente, Luisa, Ana Cerdeira-Pena, Silvia Lorenzo-Freire, and Ángeles S. Places. "Optimization in Sanger sequencing." Computers & Operations Research 109 (September 2019): 250–62. http://dx.doi.org/10.1016/j.cor.2019.05.011.

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Manoj, Kumar Saini1 Gaurav2 Harish BM3 Jitendra Kumar 3. and Kumar Sanu4. "DNA Sequencing techniques: Sanger to Next Generation Sequencing." Science World a monthly e magazine 3, no. 9 (2023): 2378–93. https://doi.org/10.5281/zenodo.8376905.

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Numerous research applications require that the order of nucleic acid residues in biological samples be determined. Numerous scientists have worked hard over the past 50 years to develop the methods and tools necessary to accomplish this feat of sequencing DNA and RNA molecules. The sequencing of small oligonucleotides has given way to sequencing millions of bases and the struggle to deduce the coding sequence of a single gene has given way to whole genome sequencing that is quick and widely accessible. The sequence of the nucleotides in a DNA molecule is determined via sequencing technology.
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Zeng, Xiaofang, Tianyu Lian, Jianhui Lin, et al. "Whole-exome sequencing improves genetic testing accuracy in pulmonary artery hypertension." Pulmonary Circulation 8, no. 2 (2018): 204589401876368. http://dx.doi.org/10.1177/2045894018763682.

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Sanger sequencing, the traditional “gold standard” for mutation detection, has been wildly used in genetic testing of pulmonary artery hypertension (PAH). However, with the advent of whole-exome sequencing (WES), few studies have compared the accuracy of WES and Sanger sequencing in routine genetic testing of PAH. PAH individuals were enrolled from Fu Wai Hospital and Shanghai Pulmonary Hospital. WES was used to analyze DNA samples from 120 PAH patients whose bone morphogenetic protein receptor type 2 (BMPR2) mutation statuses had been previously studied using Sanger sequencing. The Sanger seq
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Voulgari, E., N. Spanakis, and A. Tsakris. "Sequencing technologies for the elusive DNA molecule: let's have a closer look." ACTA MICROBIOLOGICA HELLENICA 61, no. 1 (2016): 7–16. https://doi.org/10.5281/zenodo.10020698.

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Over the past decade, impressive progress has been made in the field of genome sequencing due to the introduction of novel platforms capable of massive parallel sequencing. Herein we aim to present in a concise manner the extraordinary journey which has taken place and the following evolutionary technological pathway, beginning from the early days of Sanger sequencing and leading up to the newer second and third generation platforms. Several commonly used next generation sequencing (NGS) technologies will be presented and compared with Sanger sequencing and some of the challenges facing these
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Beck, Tyler F., James C. Mullikin, and Leslie G. Biesecker. "Systematic Evaluation of Sanger Validation of Next-Generation Sequencing Variants." Clinical Chemistry 62, no. 4 (2016): 647–54. http://dx.doi.org/10.1373/clinchem.2015.249623.

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Abstract BACKGROUND Next-generation sequencing (NGS) data are used for both clinical care and clinical research. DNA sequence variants identified using NGS are often returned to patients/participants as part of clinical or research protocols. The current standard of care is to validate NGS variants using Sanger sequencing, which is costly and time-consuming. METHODS We performed a large-scale, systematic evaluation of Sanger-based validation of NGS variants using data from the ClinSeq® project. We first used NGS data from 19 genes in 5 participants, comparing them to high-throughput Sanger seq
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Lu, Sang-Yu, Ying-Chao Chen, Jia-Lin Feng, et al. "Detection of BRAF V600E in Fine-Needle Aspiration Samples of Thyroid Nodules by Droplet Digital PCR." International Journal of Endocrinology 2022 (March 29, 2022): 1–8. http://dx.doi.org/10.1155/2022/6243696.

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Background. BRAF exon 15 p.V600E (BRAF V600E) mutation has been established as an important molecular marker for papillary thyroid carcinoma diagnosis by ultrasound-guided fine-needle aspiration biopsy (FNAB). Sanger sequencing is the gold standard for detecting BRAF V600E mutations but fails to identify low-frequency mutations. However, droplet digital PCR (ddPCR) is a popular new method for detecting low-frequency mutations. Here, we compare the efficiency of droplet digital PCR (ddPCR) and Sanger sequencing for detection of the BRAF V600E mutation in thyroid fine-needle aspiration (FNA) sam
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Zimmermann, J., H. Voss, C. Schwager, J. Stegemann, and W. Ansorge. "Automated Sanger dideoxy sequencing reaction protocol." FEBS Letters 233, no. 2 (1988): 432–36. http://dx.doi.org/10.1016/0014-5793(88)80477-0.

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Diekstra, Adinda, Ermanno Bosgoed, Alwin Rikken, et al. "Translating Sanger-Based Routine DNA Diagnostics into Generic Massive Parallel Ion Semiconductor Sequencing." Clinical Chemistry 61, no. 1 (2015): 154–62. http://dx.doi.org/10.1373/clinchem.2014.225250.

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Abstract BACKGROUND Dideoxy-based chain termination sequencing developed by Sanger is the gold standard sequencing approach and allows clinical diagnostics of disorders with relatively low genetic heterogeneity. Recently, new next generation sequencing (NGS) technologies have found their way into diagnostic laboratories, enabling the sequencing of large targeted gene panels or exomes. The development of benchtop NGS instruments now allows the analysis of single genes or small gene panels, making these platforms increasingly competitive with Sanger sequencing. METHODS We developed a generic aut
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Dissertations / Theses on the topic "Sanger sequencing"

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Thornley, David John. "Analysis of trace data from fluorescence based Sanger sequencing." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286265.

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Takara, Alexandre Hideaki. "Padronização da genotipagem da variante G202A da G6PD A- análise comparativa da relação custo-benefício entre TETRA-ARMS e sequenciamento Sanger /." Botucatu, 2018. http://hdl.handle.net/11449/165615.

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Orientador: Paulo Eduardo Martins Ribolla<br>Resumo: A deficiência da enzima Glicose-6-Fosfato Desidrogenase (G6PD) é uma anormalidade genética de alta prevalência populacional que resulta em uma menor reatividade do sistema de óxido-redução eritrocitário, geralmente sem repercussões clínicas; estima-se que mais de 300 milhões de pessoas são portadoras dessa alteração. A enzima é expressa em todos os tecidos e catalisa a primeira etapa da Via das Pentoses. Nas hemácias, essa via é de fundamental importância na manutenção do equilíbrio de seu estado redox e a deficiência dessa enzima pode favor
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Gardiner, A. R. "A genetic investigation of the muscle and neuronal channelopathies : from Sanger to next-generation sequencing." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1477237/.

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The neurological channelopathies are a group of hereditary, episodic and frequently debilitating diseases often caused by dysfunction of voltage-gated ion channels. This thesis reports genetic studies of carefully clinically characterised patient cohorts with different episodic neurological and neuromuscular disorders including paroxysmal dyskinesias, episodic ataxia, periodic paralysis and episodic rhabdomyolysis. Genetic and clinical heterogeneity has in the past, using traditional Sanger sequencing methods, made genetic diagnosis difficult and time consuming. This has led to many patients a
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Calvani, Nichola Eliza Davies. "Translocation of Fasciola hepatica via international livestock movements: development of ante-mortem molecular diagnostic tools for the identification of Fasciola spp. in livestock." Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/22464.

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Fasciolosis caused by infection with Fasciola hepatica and Fasciola gigantica is a zoonotic disease of worldwide importance, with an estimated 91 million people considered at risk of infection and livestock losses expected to exceed US $3 billion/year. Despite the significant human and animal health impacts, no test is capable of ante-mortem Fasciola spp. differentiation in areas of parasite sympatry. The aim of this thesis was to design, validate and deploy a suite of highly sensitive molecular diagnostic tools for Fasciola spp. differentiation from faecal samples to enable ante-mortem screen
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Karkar, Adnane. "Leucodystrophies : aspects génétiques et moléculaires au Maroc." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC258.

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Les leucodystrophies (LD) sont des troubles héréditaires affectant la substance blanche (SB) du système nerveux central (SNC) avec ou sans atteinte du système nerveux périphérique (SNP). Ces troubles ont en commun des anomalies de la cellule gliale ou de la gaine de la myéline. L’imagerie par résonance magnétique (IRM) représente l’outil majeur pour la détection des anomalies de la SB, ainsi l’IRM accompagnée d’un examen clinique permettent d’orienter le diagnostic étiologique. La confirmation de ce diagnostic est principalement basée sur la biologie moléculaire qui permet la détermination du
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Rocha, Bruno Garcia. "Desenvolvimento de metodologias para identificação molecular do HPV." Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/8289.

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Submitted by Livia Mello (liviacmello@yahoo.com.br) on 2016-10-14T19:44:04Z No. of bitstreams: 1 TeseBGR.pdf: 2376550 bytes, checksum: 4ee6a0f02e589ae693965093fa4f2f42 (MD5)<br>Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-11-08T18:48:20Z (GMT) No. of bitstreams: 1 TeseBGR.pdf: 2376550 bytes, checksum: 4ee6a0f02e589ae693965093fa4f2f42 (MD5)<br>Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-11-08T18:48:29Z (GMT) No. of bitstreams: 1 TeseBGR.pdf: 2376550 bytes, checksum: 4ee6a0f02e589ae693965093fa4f2f42 (MD5)<br>Made available
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Delgado, Verdugo Alberto. "Genetic Aspects of Endocrine Tumorigenesis : A Hunt for the Endocrine Neoplasia Gene." Doctoral thesis, Uppsala universitet, Endokrinkirurgi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-224111.

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Endocrine tumors arise from endocrine glands. Most endocrine tumors are benign but malignant variants exist. Several endocrine neoplasms display loss of parts of chromosome 11 or 18, produce hormones and responds poorly to conventional chemotherapeutics. The multiple endocrine neoplasia syndromes are mainly confined to endocrine tumors. This opens the question if there exists a single or several endocrine tumor genes. The aim of the study was to describe genetic derangements in endocrine tumors. Paper I: Investigation of mutational status of SDHAF2 in parathyroid tumors. SDHAF2 is located in t
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Martinsson, Caroline. "Characterisation of EGFR and KRAS mutations in non-small cell lung cancer." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-126041.

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Background: Lung cancer is the leading cause of cancer-related death and one of the most common cancer types worldwide. Epidermal growth factor receptor (EGFR) has been shown to be an important therapeutic target in non-small cell lung cancer. Kirsten rat sarcoma viral oncogene homologue (KRAS) is a downstream signalling molecule in the EGFR pathway. Lung cancer patients with EGFR mutations respond to tyrosine EGFR inhibitor therapy, in contrast, patients with KRAS mutations do not benefit of such treatment. Methods: This study investigates the frequency of EGFR and KRAS mutations in non-small
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Schimd, Michele. "Quality value based models and methods for sequencing data." Doctoral thesis, Università degli studi di Padova, 2015. http://hdl.handle.net/11577/3424144.

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First isolated by Friedrich Miescher in 1869 and then identified by James Watson and Francis Crick in 1953, the double stranded DeoxyriboNucleic Acid (DNA) molecule of Homo sapiens took fifty years to be completely reconstructed and to finally be at disposal to researchers for deep studies and analyses. The first technologies for DNA sequencing appeared around the mid-1970s; among them the most successful has been chain termination method, usually referred to as Sanger method. They remained de-facto standard for sequencing until, at the beginning of the 2000s, Next Generation Sequencing (NGS)
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Kodipad, Ahad Ahmed. "XPO1 mutations are a novel predictor of shorter time to first treatment in early stage CLL patients." Doctoral thesis, Università del Piemonte Orientale, 2021. http://hdl.handle.net/11579/128430.

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In approximately 70% of newly diagnosed cases, CLL presents at an early clinical stage and is managed with a watch & wait strategy. Until now, few clinical and molecular inform on the risk of treatment requirement. On these grounds, we aimed at identifying new molecular markers that may predict early treatment requirement and may help clinicians to better plan the watch & wait strategy in asymptomatic early stage CLL patients. 295 Binet A CLL patients who referred to our institution were subjected to next-generation-sequencing (NGS) in a panel of recurrently mutated genes in CLL. Two validatio
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Books on the topic "Sanger sequencing"

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Walsh, Richard A. Siblings with Instability. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190607555.003.0015.

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Over the past 5 years, there has been a shift in the approach to searching for a genetic diagnosis in familial ataxic syndromes. Whereas in the past, a limited but expensive search through a selection of commercially available genes using Sanger sequencing was performed, there is now widespread availability of gene panels utilizing next-generation sequencing techniques. This is an efficient and powerful approach that may achieve a diagnosis in more than 30% of patients with a familial ataxia that remain undiagnosed. However, accurate phenotyping remains critical to allow interpretation of sequ
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Book chapters on the topic "Sanger sequencing"

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Jennings, W. Bryan. "Sanger Sequencing." In Phylogenomic Data Acquisition. CRC Press, 2016. http://dx.doi.org/10.1201/9781315181431-6.

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Alphey, Luke. "Chain Termination (Sanger Dideoxy) Method." In DNA Sequencing. Garland Science, 2023. http://dx.doi.org/10.1201/9781003423737-4.

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Valencia, C. Alexander, M. Ali Pervaiz, Ammar Husami, Yaping Qian, and Kejian Zhang. "Sanger Sequencing Principles, History, and Landmarks." In SpringerBriefs in Genetics. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-9032-6_1.

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Torres, Anthony, and Reginald Gaudino. "Targeted Amplicon Genotyping by Sanger Sequencing." In Methods in Molecular Biology. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4642-7_14.

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García-Sancho, Miguel, and James Lowe. "The Funnelling Effect of the Sanger Institute." In A History of Genomics across Species, Communities and Projects. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-06130-1_4.

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AbstractIn this chapter, we focus on an institution that represents the exclusivity of the International Human Genome Sequencing Consortium (IHGSC) while also contributing to the ‘funnelling effect’ that shaped it: the Sanger Institute. After situating its origins in the sequencing of the worm Caenorhabditis elegans, we portray the Sanger Institute as a driver in the formation of the IHGSC and as an advocate for the concentration of its membership into a few high-throughput sequencing centres. The intensification of this genome centre model, alongside competition with the corporate sequencing
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Jamal, Muhsin, Sana Raza, Sayed Muhammad Ata Ullah Shah Bukhari, et al. "First Generation–The Sanger Shotgun Approach." In Sequencing Technologies in Microbial Food Safety and Quality. CRC Press, 2021. http://dx.doi.org/10.1201/9780429329869-11.

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Nielsen, Kåre Lehmann. "Low-Cost-Medium Throughput Sanger Dideoxy Sequencing." In Methods in Molecular Biology. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-454-4_5.

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Barzon, Valentina, Ilaria Ferrarotti, and Stefania Ottaviani. "Sanger and Next-Generation Sequencing of AAT." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3605-3_6.

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Xie, Xianrong, Xingliang Ma, and Yao-Guang Liu. "Decoding Sanger Sequencing Chromatograms from CRISPR-Induced Mutations." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_3.

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Ballard, David. "Analysis of Mitochondrial Control Region Using Sanger Sequencing." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3597-0_12.

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Conference papers on the topic "Sanger sequencing"

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Galieva, Gulnaz, Polina Kuryntseva, Polina Galitskaya, and Svetlan Selivanovskay. "ASSESSMENT OF THE BIODEGRADABILITY OF CATIONIC FLOCCULANTS BY MICROORGANISMS ISOLATED FROM SEWAGE SLUDGE OBTAINED ON WASTEWATER TREATMENT PLANTS." In 24th SGEM International Multidisciplinary Scientific GeoConference 2024. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/5.1/s20.03.

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Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation of substantial volumes of sewage sludge in these plants. To manage this sludge,
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Jain, Luke A., Chase Williamson, John R. Spear, David L. Olson, Brajendra Mishra, and Russell D. Kane. "Mechanistic MIC Evaluation of Linepipe in Ethanol and Acetic Acid Solutions." In CORROSION 2015. NACE International, 2015. https://doi.org/10.5006/c2015-05708.

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Abstract Recent field reports have suggested that microbiologically influenced corrosion (MIC) is occurring at biologically favorable sites in equipment handling ethanol fuels. Recent laboratory corrosion testing has demonstrated the susceptibility of engineering alloys to corrosion in ethanol solutions containing microbes cultivated from an ethanol fuel terminal. This suggests handling and transportation of larger volumes of ethanol and ethanol fuel blends may increase the likelihood of MIC related failures. Mitigating this threat will likely increase the cost of integrity management of fuel
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Kun Sun, Yuet-Ping Yuen, Huating Wang, and Hao Sun. "The Online Diagnosis System for sanger sequencing based genetic testing." In 2014 International Conference on Big Data and Smart Computing (BIGCOMP). IEEE, 2014. http://dx.doi.org/10.1109/bigcomp.2014.6741404.

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Chanyshev, M. D., N. V. Vlasenko, I. A. Kotov, K. F. Khafizov, and V. G. Akimkin. "HIGH THROUGHPUT DNA SEQUENCING OF HEPATITIS B VIRUS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-266.

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It was shown that Hepatitis B Virus (HBV) genotype and individual mutations influence the course of the disease. There is a need for a simple and reliable method for sequencing the entire genome of hepatitis B virus. We have developed an NGS amplification panel for hepatitis B virus genome sequencing. The panel was validated using Sanger sequencing. More than 300 HBV samples were sequenced and genotypes and mutations described in the literature were identified.
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Boshlyakov, G. S., E. V. Grishina, A. O. Paiberdin, et al. "THE ELABORATION OF A HIGHLY EFFECTIVE MICROBIOLOGICAL FERTILISER BASED ON STRAINS OF NITROGEN-FIXING BACTERIA." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-52.

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Mineral fertilizers lose their effectiveness. It improved by adding microbiological fertilizers. Six bacterial strains were isolated and characterized, and their agronomic potential was studied. A dense nutrient medium was optimized. Two strains were found to enter into antagonistic relationships with P. chlororaphis 449. Spectrofluorometry showed the accumulation of siderophores. Strains were determined using spectrometry and Sanger sequencing methods.
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Septiyanto, Abdullah Faqih, Yuni Fitri Cahyaningsih Cahyono, Riyanarto Sarno, et al. "An Improved Method for Prioritizing Polymerase Chain Reaction (PCR) Primer Design in Sanger Sequencing." In 2022 6th International Conference on Information Technology, Information Systems and Electrical Engineering (ICITISEE). IEEE, 2022. http://dx.doi.org/10.1109/icitisee57756.2022.10057821.

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Pelak, Kimberly, Jennifer Wright, Zhenyu Yan, et al. "Abstract 3509: Complementary analysis of LKB1/STK11 mutation and protein expression status using next-generation sequencing, Sanger sequencing and immunohistochemistry." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-3509.

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Skevington, Jeffrey H. "Unravelling the phylogeny of the lower Cyclorrhapha using morphology, Sanger sequencing, and anchored phylogenetic data." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94498.

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Allice, T., F. Cerutti, MG Milia, et al. "P-219 Success rate of HIV DNA Sanger sequencing in PBMC and whole blood samples." In Abstracts from the 16° Italian Conference on AIDS and Antiviral Research. BMJ Publishing Group Ltd, 2024. http://dx.doi.org/10.1136/sextrans-icar-2024.271.

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Rodrigues, GM, FCZ Volpato, PL Wink, RM Paiva, AL Barth, and F. Paris. "VARIANTES DE PREOCUPAÇÃO DO SARS-COV-2: IDENTIFICAÇÃO PRESUNTIVA POR MEIO DA ANÁLISE POR SEQUENCIAMENTO DE SANGER DA REGIÃO DE LIGAÇÃO AO DOMÍNIO DO RECEPTOR (RBD) DO GENE S." In Resumos do 54º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2022. http://dx.doi.org/10.5327/1516-3180.140s1.6130.

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Objetivo: Caracterizar as VOCs por meio de sequenciamento de sanger da região RBD, bem como estabelecer a concordância com resultados obtidos por sequenciamento completo do genoma viral através da técnica de whole-genome sequencing (WGS). Método: O RNA viral de amostras positivas foi isolado e submetido à transcrição reversa, utilizando o kit GoScript™ Reverse Transcriptase. A PCR foi realizada utilizando primers que flanqueiam a região genômica RBD do vírus, formando um fragmento de 1006 pares de base. Eletroforese em gel de agarose 1% foi realizada para confirmar a amplificação, e o amplific
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Reports on the topic "Sanger sequencing"

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Ghanim, Murad, Joe Cicero, Judith K. Brown, and Henryk Czosnek. Dissection of Whitefly-geminivirus Interactions at the Transcriptomic, Proteomic and Cellular Levels. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7592654.bard.

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Our project focuses on gene expression and proteomics of the whitefly Bemisia tabaci (Gennadius) species complex in relation to the internal anatomy and localization of expressed genes and virions in the whitefly vector, which poses a major constraint to vegetable and fiber production in Israel and the USA. While many biological parameters are known for begomovirus transmission, nothing is known about vector proteins involved in the specific interactions between begomoviruses and their whitefly vectors. Identifying such proteins is expected to lead to the design of novel control methods that i
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