Dissertations / Theses on the topic 'Scat DNA'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 15 dissertations / theses for your research on the topic 'Scat DNA.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Stover, Rachyl-anne. "Description of the dietary breadth and overlap of the translocated Shark Bay rufous hare-wallaby (Lagorchestes hirsutus) and banded hare-wallaby (Lagostrophus fasciatus) using scat DNA." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2025. https://ro.ecu.edu.au/theses/2922.
Full textAlamri, Sarah. "COMPARATIVE ANALYSIS OF SOYBEAN (GLYCINE MAX) ACCESSIONS USING INTER SIMPLE SEQUENCE REPEAT (ISSR) AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS." Thesis, Laurentian University of Sudbury, 2014. https://zone.biblio.laurentian.ca/dspace/handle/10219/2201.
Full textWang, Ying-Hui. "Molecular interaction of zinc finger domain : study of androgen receptor DNA binding domain and SCA7 domain of Ataxin7 by NMR." Strasbourg, 2010. http://www.theses.fr/2010STRA6018.
Full textThe androgen receptor (AR) is a ligand-activated transcriptional factor and a member of the nuclear receptor super family. AR shares a common structural and functional architecture with other members of nuclear receptors. The DNA binding domain of AR (ARDBD) binds to specific response elements as a homodimer. In the clinic, certain mutations in AR are associated with the progression of prostate cancer and have consequences for the treatment of patients with advanced prostate cancer. Previous studies showed that the mutation T575A, locating in the DNA binding domain, enhances the transcriptional activity regulated by full-length AR on promoters containing the non-specific response element compared to the wild type domain does not. These differences prompted us to study the molecular mechanism of ARDBD wild type and the T575A mutant. Structures of ARDBD wild type and T575A mutant revealed high similarity. However, dynamic behavior showed distinct differences between wild type and T575A mutant domains. The protonation state of H570 in ARDBD was found to be differed by the mutation. This loss of charge of H570 results in changes in transcriptional activity of AR. .
Queiroz, Vagner Tebaldi de. "Obtenção de primers microssatélite e desenvolvimento, validação e mapeamento de marcadores SCAR em feijoeiro-comum." Universidade Federal de Viçosa, 2004. http://www.locus.ufv.br/handle/123456789/8784.
Full textMade available in DSpace on 2016-10-05T19:00:01Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1608244 bytes, checksum: 73ced9bf50274d34a09508edd0498859 (MD5) Previous issue date: 2004-09-20
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Embora apresente várias limitações, a técnica de RAPD ainda representa a principal ferramenta utilizada no Programa de Melhoramento Genético do Feijoeiro- comum BIOAGRO/UFV. Para contornar os problemas apresentados por esta técnica, a estratégia utilizada por vários pesquisadores tem sido a conversão dos marcadores RAPD em marcadores SCAR e o desenvolvimento de primers microssatélite. Assim, no presente trabalho foram desenvolvidos 5 primers SCAR para mancha-angular, 5 primers para ferrugem e 7 primers para antracnose. Os primers desenvolvidos para mancha-angular derivaram-se dos marcadores OPM02 425 , OPBA16 669 , OPH13 490 e OPH14+AA19 400 e foram mapeados, em acoplamento, a 5,3, 7,1, 5,6 e 10,1 cM, dos respectivos genes de resistência. Os primers desenvolvidos para ferrugem originaram-se dos marcadores OPAE19 890 , OPX11 550 , OPAJ18+AH10 700 , mas apenas o primer sAE19 foi validado. Este foi mapeado, em repulsão, a 1,0 cM do gene de resistência Ur-11. Os primers para antracnose foram desenvolvidos, a partir da seqüência dos fragmentos OPAZ20 940 , OPY20 830 , OPC08 900 , OPZ04 560 , OPB03 1800 , OPZ09 950 , OPH18 830 . Destes, apenas os primers sAZ20, sY20, sC08, sZ04 foram mapeados, em acoplamento, a 7,1, 1,2, 7,8 e 2,9 cM, dos respectivos genes de resistência. Os primers microssatélite foram desenvolvidos, a partir das seqüências de fragmentos de DNA genômico de 3 pequenas bibliotecas enriquecidas. As bibliotecas enriquecidas para as repetições GGC, CCA e AT permitiram a seleção de 79, 172 e 50 clones positivos, respectivamente. Do total de 301 clones que foram identificados, 153 já foram seqüenciados. Observou-se que 40 (26%) clones seqüenciados apresentaram fragmentos de DNA contendo microssatélite, os quais variaram quanto ao tipo, número e tamanho. As seqüências apresentaram repetições de di-, tri-, tetra- e até pentanucleotídeos. Em um mesmo fragmento, foram encontrados até 3 microssatélites. Entre as seqüências analisadas, foram observadas repetições perfeitas, imperfeitas e compostas, variarando entre 12 e 24 pb. Até o momento, foram desenhados e testados 10 pares de primers, sendo um deles originado da seqüência do marcador RAPD OPAZ20 940 . Destes, apenas 5 pares (FCctc001, FCggc001, FCccg001, FCccg002 e FCgca001) amplificaram fragmentos com tamanho esperado e bem definidos. Entretanto, esses primers foram monofórficos, quando testados entre diferentes cultivares andinos e mesoamericanos. Os primers SCAR, desenvolvidos e validados no presente trabalho, foram testados juntamente com 45 pares de primers microssatélite comerciais, entre os genitores de uma população de 154 RIL ́s. Dos primers selecionados, 7 foram mapeados em 10 grupos de um mapa parcial de ligação já existente e 1 permaneceu não-ligado. O mapa de ligação sofreu pequena variação no tamanho (247,8/252 cM) e no número de grupos de ligação (9/10 grupos). Entretanto, pela análise de variância foram constatadas associações significativas desses marcadores com diferentes características quantitativas. As associações mais significativas foram constatadas por meio de análises de regressão stepwise, as quais promoveram alteração no valor de R 2 da regressão múltipla para algumas das características. As características MAT (número de dias até a maturação), VAPLA (número médio de vagens por planta), SEPLA (número médio de sementes por planta) e PRVAG (produção média por vagem), que apresentavam valores de R 2 de 40,14; 28,99; 14,03 e 17,13 , tiveram um aumento para 44,30; 34,53; 21,92 e 26,03, respectivamente. A inclusão do marcador sH13 no GL 07 possibilitou a identificação de um novo QTL para a característica VAPLA. Este marcador também mostrou-se associado ao QTL, anteriormente, descrito para SEPLA. O marcador BM165 foi mapeado no GL 02 e mostrou-se associado a 4 QTLs diferentes, identificados para as características MAT, P100 (peso de 100 sementes), SEPLA e PRVAG.
The RAPD technique represents the major molecular tool for assisting the common bean breeding program of the BIOAGRO/UFV, in spite of its limitations. The conversion of the RAPD markers into SCAR ones, as well as the development of SSR primers are the strategy adopted by several researchers in order to relieve these problems. Therefore, 5 primers SCAR to angular leaf spot, 5 primers to rust and 7 primers to anthracnose were developed. The primers developed to angular leaf spot were derived from the RAPD markers OPM02 425 , OPBA16 669 , OPH13 490 and OPH14+AA19 400 and were mapped, in coupling phase, at 5.3, 7.1, 5.6 and 10.1 cM, from their respective resistance genes. The primers developed to rust were originated from the RAPD markers OPAE19 890 , OPX11 550 , OPAJ18+AH10 700 , although only the primer sAE19 was validated. It was mapped, in repulsion, at 1.0 cM distance from the resistance gene Ur-11. The primers developed for anthracnose were obtained from the fragment sequences of OPAZ20 940 , OPY20 830 , OPC08 900 , OPZ04 560 , OPB03 1800 , OPZ09 950 , OPH18 830 , from wich, only the primers sAZ20, sY20, sC08, sZ04 were validated. They were mapped, in coupling phase, at 7.1, 1.2, 7.8 and 2.9 cM, from their respective resistance genes. The SSR primers were developed from the sequences of the genomic DNA fragments obtained from three small enriched libraries. The libraries were enriched for the repetitions GGC, CCA and AT and made possible the selection of 79, 172 e 50 positive clones, respectively. From the total of 301 positive and identified clones, 153 were sequenced. From these clones, 40 (26%) presented DNA fragments containing microsatellite that showed variations for type, number, and size. The sequences showed repetitions of two, three, four, and five nucleotides. In the same DNA fragment, a total up to three microsatellites were found. Among the analyzed sequences, some perfect, imperfect, and compound repetitions ranging from 12 to 24 bp were found. Until this moment, only 10 pairs of primers were designed and tested; one of them was originated from the RAPD marker OPAZ20 940 . From those, only 5 pairs (FCctc001, FCggc001, FCccg001, FCccg002 e FCgca001) amplified the well-defined fragments with the expected size. However, those primers were monomorphics ones, when tested among different andean and mesoamerican cultivars. The developed and validated SCAR primers were tested together with 45 pairs of commercial SSR primers between the genitors of 154 RIL populations. From the selected primers, 7 were mapped into 10 groups of a partial linkage map already available in the lab, whereas one stayed discoupled. The linkage map was slightly altered in its size (247.8/252 cM) and number of linkage groups (9/10 groups). Variance analysis detected siginificative associations of these markers with different quantitative traits. The most significative associations were detected by stepwise regression analysis that promoted alteration in R 2 values of the multiple regression analysis for some traits. The R 2 values of the traits MAT (the number of days until the bean maturation), VAPLA (the average pod number per plant), SEPLA (the average seed number per plant) and PRVAG (the average yield per pod) increased from 40.14, 28.99, 14.03 and 17.13 to 44.30, 34.53, 21.92 and 26.03, respectively. The marker sH13 included into LG 07 allowed for the identification of a new QTL for the trait VAPLA. This marker also showed to be associated with the QTL previously described for SEPLA. The marker BM165 was mapped in LG 02 and showed to be associated with four different QTLs identified for the traits MAT, P100 (100-seed weight), SEPLA and PRVAG.
Tese importada do Alexandria
Oliete, Calvo Paula. "Study of the SAGA deubiquitination module: identification of new modulators and its implication on Spinocerebellar Ataxia Type 7." Doctoral thesis, Universitat Politècnica de València, 2017. http://hdl.handle.net/10251/86155.
Full textLa regulación de la cromatina a través de modificaciones epigenéticas es un paso fundamental durante el control de la expresión génica en células eucariotas. La participación de diferentes factores tales como chaperonas de histonas, complejos de remodelación de la cromatina y complejos modificadores de histonas, regulan la dinámica de la cromatina y garantizan el correcto metabolismo de los transcritos que necesitan ser exportados al citoplasma. De esta forma, las modificaciones postraduccionales que incluyen la monoubicuitinación de la histona H2B (H2Bub1) y la metilación de la histona H3 representan un "cross-talk" de histonas la cual es requerida para la integridad de la cromatina y la transcripción. Además, la transición de H2Bub1 a su forma desubicuitinada por Ubp8, la enzima DUB del complejo co-activador SAGA (Spt-Ada-Gcn5-acetiltranferasa), es necesaria para obtener una expresión génica correcta. En este trabajo, se demuestra que la chaperona de histona Asf1 y la proteína de unión a Ran, Mog1, participan en el mantenimiento de los niveles de H2Bub1. Se demuestra que Mog1 es necesaria para la trimetilación de la histona H3 en la lisina 4 (H3K4me3), actuando como un modulador del "cross-talk" de histonas. El papel de Mog1 en la expresión génica también se demuestra por sus interacciones físicas y genéticas con factores de transcripción, incluyendo los complejos SAGA y COMPASS. Además, demostramos que Mog1 interactúa genéticamente con subunidades de TREX-2 y afecta a la exportación de mRNAs. Durante este trabajo, también nos hemos centrado en la comprensión de los mecanismos moleculares que envuelven a la Ataxia Espinocerebelosa Tipo 7 (SCA7), que es una enfermedad rara causada por una repetición de aminoácidos glutamina (Q) dentro del componente del DUBm, ATXN7. Por lo tanto, nuestro interés se ha dirigido hacia el estudio de nuevos mecanismos que desencadenan SCA7, como la actividad DUB del complejo SAGA, las interacciones proteína-proteína y los perfiles metabólicos.
La regulació de la cromatina a través de modificacions epigenètiques és un pas fonamental durant el control de l'expressió gènica en cèl·lules eucariotes. La participació de diferents factors tals com chaperones d'histones, complexos de remodelació de la cromatina i complexos modificadors d'histones, regulen la dinàmica de la cromatina i garanteixen el correcte metabolisme dels transcrits que necessiten ser exportats al citoplasma. D'aquesta forma, les modificacions postraduccionals que inclouen la monoubicuitinació de la histona H2B (H2Bub1) i la metilació de la histona H3 representen un "cross-talk" d'histones la qual és requerida per a la integritat de la cromatina i la transcripció. A més, la transició d'H2Bub1 a la seua forma desubicuitinada per Ubp8, l'enzim DUB del complex co-activador SAGA (Spt-Ada-Gcn5-acetiltranferasa), és necessària per a obtenir una expressió gènica correcta. En aquest treball, es demostra que la chaperona de histona Asf1 i la proteïna d'unió a Ran, Mog1, participen en el manteniment dels nivells d'H2Bub1. Es demostra que Mog1 és necessària per a la trimetilació de la histona H3 en la lisina 4 (H3K4me3), actuant com un modulador del "cross-talk" d'histones. El paper de Mog1 en l'expressió gènica també es demostra per les seues interaccions físiques i genètiques amb factors de transcripció, incloent els complexos SAGA i COMPASS. A més, vam demostrar que Mog1 interactua genèticament amb subunitats de TREX-2 i afecta a l'exportació de mRNA. Durant aquest treball, també ens hem centrat en la comprensió dels mecanismes moleculars que envolten a l'Atàxia Espinocerebelosa Tipus 7 (SCA7), que és una malaltia rara causada per una repetició d'aminoàcids glutamina (Q) dins del component del DUBm, ATXN7. Per tant, el nostre interès s'ha dirigit cap a l'estudi de nous mecanismes que desencadenen SCA7, com l'activitat DUB del complex SAGA, les interacciones proteïna-proteïna i els perfils metabòlics.
Oliete Calvo, P. (2017). Study of the SAGA deubiquitination module: identification of new modulators and its implication on Spinocerebellar Ataxia Type 7 [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/86155
TESIS
Silva, Luiz Henrique da. "O fenômeno de lente térmica em amostras de DNA livre circulante de pacientes com malignidade e sãos, investigado por meio da técnica de varredura-Z." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-13042017-014509/.
Full textIn the present study plasma samples with cell-free DNA were investigated by means of the Z-Scan technique. This is a powerfull technique in determining parameters of different materials, such as liquid crystals, ferrofluids and biological compounds. This experiment is performed by focusing a Gaussian profile laser beam on a sample. As the sample approaches the focus of the lens, the intensity of the beam increases and reaches its maximum value at the focal point, then decreases to points distant from the focus. In the region near the focal point non-linear phenomena are amplified. It has recently been demonstrated that high levels of plasma cell-free DNA occur frequently in patients with various cancers and can be used to discriminate patients with malignancy from healthy donors. The cell-free DNA samples, submitted to the Z-Scan experiment, provided optical responses due to the thermal lens phenomenon. The results revealed that the thermal lens amplitude of samples extracted from the plasma of patients with malignancy differs from that of healthy donors. The Z-Scan technique was more advantageous than other biological ones because it revealed a greater difference between the studied groups and has the character of detecting structural changes in cell-free DNA.
Shrestha, Ujjwal. "Automatic Liver and Tumor Segmentation from CT Scan Images using Gabor Feature and Machine Learning Algorithms." University of Toledo / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1522411364001198.
Full textMokhele, Tshediso Andrew. "The application of DNA fingerprinting and marker-assisted backcross selection in breeding for sunflower high oleic acid content lines / by Tshediso Andrew Mokhele." Thesis, North-West University, 2013. http://hdl.handle.net/10394/9793.
Full textThesis (MSc (Botany))--North-West University, Potchefstroom Campus, 2013.
Nepraš, Ondřej. "Aplikace Lean Production." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2011. http://www.nusl.cz/ntk/nusl-229974.
Full textBenda, Ondřej. "Optimalizace činnosti měrového střediska." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2008. http://www.nusl.cz/ntk/nusl-228196.
Full textYang, Wen-Hui, and 楊雯惠. "Development of DNA Profiles and SCAR Marker of Southern India Type Vetiver (Vetiveria zizanioides (L.) Nash)." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/07540169104274716263.
Full text國立臺灣大學
農藝學研究所
91
A collection of 25 worldwide representative samples was used to assess the genetic diversity of Southern India type vetiver as well as the development of technique for variety identification. The AFLP marker system was used to study the genetic diversity of Southern India type vetiver. The results indicated the reproducibility of the banding information of the AFLP marker system was 99.5%. Four sets of primers combinations were selected in the analysis and yielded 260 polymorphic bands. One hundred and sixty five polymorphic bands were selected to estimate the genetic distance of the vetiver, which was ranging from 0.09 to 0.64. A cluster analysis with UPGMA methodology of the samples shown the samples from outside India was belong to the group “Sunshine”. The DNA profile information of each one of the Southern India vetiver was established from the AFLP and RAPD polymorphic bands. The three of the four primer combinations in the AFLP analysis were able to establish DNA profile for each one of the samples alone. While the RAPD marker system required information from at least three primers to distinguish the cultivars. Twenty-six and thirty-nine unique bands from RAPD and AFLP, respectively, were identified from the revealed polymorphic bands in the analyses. Ten unique bands were chosen in the development of SCAR marker for the identification of the cultivars in order to improve the accuracy of the variety identification. Eight of the designed SCAR markers were shown to be able to positively identify the corresponded cultivars as in RAPD.
Lewis, Thomas Wade Stakesby. "Dendrimers as drug and gene delivery vectors : a self consistent field theory study." 2013. http://hdl.handle.net/2152/21615.
Full texttext
Tsai, Tzung-Yu, and 蔡宗育. "Genetic Diversity in butterhead and crisphead lettuce (Lactuca sativa L.) by using Morphological Traits, Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Regions (SCAR) Markers." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/59226251002783909910.
Full text國立中興大學
園藝學系所
96
The genetic diversity of 31 butterhead (BUT) and 26 crisphead (CRP) accessions were evaluated by morphological traits, RAPD (Random Amplified Polymorphic DNA) and SCAR (Sequence Characterized Amplified Regions). Data for 19 morphological traits were subjected to a genetic diversity analysis, after which a UPGMA cluster analysis was performed. The 57 accessions were clustered into 2 groups according to type with genetic similarity of 0.38. The first group included 15 accessions form Tainan and 3 accessions form the National Germplasm of the USA, which were clustered into 5 subgroups with the similarity beyond 0.61. The second group included 10 crisphead and 26 butterhead accessions, which were clustered into 6 subgroups with the similarity beyond 0.70. A total of 271 RAPD bands, with a mean band of 9.3 for each primer, were generated using 29 out of 130 primers in the RAPD analysis. The polymorphism was 53.9%. All accessions were clustered into 3 groups in the RAPD analysis. The first group included 13 crisphead accessions form the Taiwan agricultural research institute(TARI) with the similarity 0.86. The second group including 15 accessions form the Tainan district agricultural research and extention station(TDARES)、3 accessions form USA and 3 butterhead accessions with the similarity 0.88. The first, second and fourth subgroups included crisphead accessions. The third and fifth subgroups included 3 butterhead accessions. The third group was composed of 23 butterhead accessions with a the similarity of 0.82. In analyzing results from SCAR of butterhead and crisphead accessions, a total of 100 bands were generated using 21 out of 51 primer pairs. The means of bands generated by a primer pairs was 4.8 and the polymorphism was 73%. The 57 accessions were clustered into 3 groups. The first group included 13 crisphead accessions form the TARI with the similarity 0.76. The second group including 15 accessions form TDARES with a similarity of 0.88. The third group was composed of 26 butterhead and 3 accessions the USA with a similarity of 0.88. The analysis of RAPD and SCAR result in the main group of the dendrogram showed a similarity of 0.78~0.86 based on RAPD markers and which the SCAR markers displayed a similarity 0.68~0.78. The analysis of RAPD and SCAR result in the subgroup of the dendrogram showed a similarity of 0.84~0.96 based on RAPD markers and which the SCAR markers displayed a similarity 0.88~0.97. Lettuce germplasm could be identified by morphological characteristics such as leaf color, leaf texture and head type. The molecular markers could be used to cluster accessions into groups by SCAR and subgroups by RAPD successfully.
MARIESCHI, Matteo. "Identificazione di possibili sofisticazioni in preparati commerciali di origano Mediterraneo ed analisi genetica di Origanum spp. mediante marcatori molecolari genomici: Random Amplified Polymorphic DNA (RAPD) e Sequence Characterized Amplified Region (SCAR)." Doctoral thesis, 2010. http://hdl.handle.net/11381/2306929.
Full textBrandfaß, Christoph. "Establishment and application of real-time PCR-based methods to study the epidemiology of Fusarium Head Blight." Doctoral thesis, 2006. http://hdl.handle.net/11858/00-1735-0000-000D-F220-2.
Full text