Relevant bibliographies by topics / Scratch Assay

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Scratch Assay'

Author: Grafiati
Published: 4 June 2021
Last updated: 31 January 2023

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Journal articles on the topic "Scratch Assay"

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Alishahedani, Mohammadali E., Manoj Yadav, Katelyn J. McCann, Portia Gough, Carlos R. Castillo, Jobel Matriz, and Ian A. Myles. "Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing." PLOS ONE 16, no. 6 (June 18, 2021): e0253669. http://dx.doi.org/10.1371/journal.pone.0253669.

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The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted.
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Vargas, Andrea, Marc Angeli, Chiara Pastrello, Rosanne McQuaid, Han Li, Andrea Jurisicova, and Igor Jurisica. "Robust quantitative scratch assay." Bioinformatics 32, no. 9 (December 31, 2015): 1439–40. http://dx.doi.org/10.1093/bioinformatics/btv746.

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Gough, Wendy, Keren I. Hulkower, Renee Lynch, Patrick Mcglynn, Mark Uhlik, Lei Yan, and Jonathan A. Lee. "A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to the Scratch Assay." Journal of Biomolecular Screening 16, no. 2 (February 2011): 155–63. http://dx.doi.org/10.1177/1087057110393340.

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Cell migration is a key phenotype for a number of therapeutically important biological responses, including angiogenesis. A commonly used method to assess cell migration is the scratch assay, which measures the movement of cells into a wound made by physically scoring a confluent cell monolayer to create an area devoid of cells. Although this method has been adequate for qualitative characterization of migration inhibitors, it does not provide the highly reproducible results required for quantitative compound structure-activity relationship evaluation because of the inconsistent size and placement of the wound area within the microplate well. The Oris™ Cell Migration Assay presents a superior alternative to the scratch assay, permitting formation of precisely placed and homogeneously sized cell-free areas into which migration can occur without releasing factors from wounded or dead cells or damaging the underlying extracellular matrix. Herein the authors compare results from the scratch and Oris™ cell migration assays using an endothelial progenitor cell line and the Src kinase inhibitor dasatinib. They find that using the Acumen™ Explorer laser microplate cytometer in combination with the Oris™ Cell Migration Assay plate provides a robust, efficient, and cost-effective cell migration assay exhibiting excellent signal to noise, plate uniformity, and statistical validation metrics.
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Bobadilla, Ana Victoria Ponce, Jazmine Arévalo, Eduard Sarró, Helen M. Byrne, Philip K. Maini, Thomas Carraro, Simone Balocco, Anna Meseguer, and Tomás Alarcón. "In vitro cell migration quantification method for scratch assays." Journal of The Royal Society Interface 16, no. 151 (February 2019): 20180709. http://dx.doi.org/10.1098/rsif.2018.0709.

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The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. The assay can also be used to evaluate therapeutic compounds before clinical use. Current quantification methods of scratch assays deal poorly with irregular cell-free areas and crooked leading edges which are features typically present in the experimental data. We introduce a new migration quantification method, called ‘monolayer edge velocimetry’, that permits analysis of low-quality experimental data and better statistical classification of migration rates than standard quantification methods. The new method relies on quantifying the horizontal component of the cell monolayer velocity across the leading edge. By performing a classification test on in silico data, we show that the method exhibits significantly lower statistical errors than standard methods. When applied to in vitro data, our method outperforms standard methods by detecting differences in the migration rates between different cell groups that the other methods could not detect. Application of this new method will enable quantification of migration rates from in vitro scratch assay data that cannot be analysed using existing methods.
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Gotsulyak, N. Ya, V. R. Kosach, O. V. Cherednyk, I. O. Tykhonkova, and A. I. Khoruzhenko. "Optimization of cell motility evaluation in scratch assay." Biopolymers and Cell 30, no. 3 (May 20, 2014): 223–28. http://dx.doi.org/10.7124/bc.00089d.

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Putri, Widya Wira, Nadya Muthia Risky, Nungki Pramita Sari, Rizka Kurnia Gemilang, Rufaida Mudrika, Indra Kusuma, Restu Syamsul Hadi, and Yurika Sandra. "Dimethyl Sulfoxide Inhibits CD271+ Migration in Scratch Assay." Advanced Science Letters 23, no. 7 (July 1, 2017): 6881–83. http://dx.doi.org/10.1166/asl.2017.9423.

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Jana, Snehasis, and Mahendra Kumar Trivedi. "Wound Healing Activity of Consciousness Energy Healing Treatmenton HFF-1 Cells and DMEM Using Scratch Assay." Investigative Dermatology and Venereology Research 4, no. 1 (December 26, 2018): 50–54. http://dx.doi.org/10.15436/2381-0858.18.2036.

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The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This methodwas used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEMwassignificantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect® has the significant capacity and wide implications in wound healing activityvia cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.
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Minadakis, Georgios, Emmanouil Angelakis, Dimosthenis Chochlakis, Yannis Tselentis, and Anna Psaroulaki. "Cat-Scratch disease in Crete: an update." Infectious Disease Reports 3, no. 2 (December 5, 2011): 15. http://dx.doi.org/10.4081/idr.2011.3210.

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There are few epidemiological and clinical studies about the presence of cat scratch disease (CSD) on the island of Crete. The objective of this study was to analyze a large number of patients with suspected CSD to define the frequency of <em>Bartonella</em> infections in Crete. From January 2005 to October 2008, we studied patients with suspected CSD from hospitals in Crete. Sera of the referred patients were tested by immunofluorescence assay (IFA). For some patients, we also received lymph nodes and blood samples that we tested for the presence of <em>Bartonella henselae</em> by molecular assays. Overall, we tested 507 serum samples and we found 56 (11%) cases of CSD. PCR assay was positive for 2 patients; one had a <em>B. henselae</em> positive lymph node and the other a positive whole blood sample. Significantly more CSD cases (62.5%, 35 of 56) were reported in children than in infants and adults (P&lt;0.05). Moreover, we identified that most cases of CSD occurred between May and September (P=0.002) and December and January. CSD is prevalent in Crete and is mostly associated with an increase in outdoor activity.
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Liu, Lili, Zhiying Xu, Binbin Yu, Li Tao, and Ying Cao. "Berbamine Inhibits Cell Proliferation and Migration and Induces Cell Death of Lung Cancer Cells via Regulating c-Maf, PI3K/Akt, and MDM2-P53 Pathways." Evidence-Based Complementary and Alternative Medicine 2021 (July 8, 2021): 1–20. http://dx.doi.org/10.1155/2021/5517143.

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Berbamine (BBM) is a natural product isolated from Berberis amurensis Rupr. We investigated the influence of BBM on the cell viability, proliferation, and migration of lung cancer cells and explored the possible mechanisms. The cell viability and proliferation of lung cancer cells were evaluated by MTT assay, EdU assay, and colony formation assay. Migration and invasion abilities of cancer cells were determined through wound scratch assay and Transwell assay. Cell death was evaluated by cell death staining assay and ELISA. The expressions of proteins were evaluated using western blot assay. A xenograft mouse model derived from non-small-cell lung cancer cells was used to detect the effect of BBM on tumor growth and metastasis in vivo. Both colony formation and EdU assays results revealed that BBM (10 μM) significantly inhibited the proliferation of A549 cells ( P < 0.001 ). BBM (10 μM) also significantly inhibited the migration and invasion ability of cancer cells in wound scratch and Transwell assays. Trypan blue assay and ELISA revealed that BBM (20 μM) significantly induced cell death of A549 cells. In xenograft mouse models, the tumor volume was significantly smaller in mice treated with BBM (20 mg/kg). The western blotting assay showed that BBM inhibited the PI3K/Akt and MDM2-p53 signaling pathways, and BBM downregulated the expression of c-Maf. Our results show that BBM inhibits proliferation and metastasis and induces cell death of lung cancer cells in vitro and in vivo. These effects may be achieved by BBM reducing the expression of c-Maf and regulating the PI3K/Akt and MDM2-p53 pathways.
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Scholz, H. M., I. Aykara, K. Frommer, S. Rehart, U. Müller-Ladner, and E. Neumann. "AB0044 ENDOTHELIAL CELL AND RHEUMATOID ARTHRITIS SYNOVIAL FIBROBLAST MIGRATION AND ADHESION ARE ALTERED BY ACTIVIN/FOLLISTATIN." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 1054.2–1055. http://dx.doi.org/10.1136/annrheumdis-2021-eular.271.

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Background:Activin A and follistatin belong to an anti-inflammatory auto-regulatory cycle. Patients with rheumatoid arthritis (RA) have increased activin A levels in the synovial fluid and tissue. During inflammation, activin A is released systemically, then inducing its antagonist follistatin. This negative feedback is active in different cell types but not RA synovial fibroblasts (SF). Fibroblasts interact with endothelial cells in inflamed tissues inducing angiogenesis.Objectives:Evaluation of the role of activin A and follistatin on RASF and endothelial cell interactions.Methods:RA synovium was used for RASF isolation, HUVEC were commercially obtained. RASF and HUVEC were stimulated in mono- and coculture. Direct: RASF together with HUVEC; indirect: inserts with HUVEC separated by a membrane from RASF. Stimuli: activin A 15ng/ml, follistatin 500ng/ml, IL-1β 1ng/ml. Proliferation was analyzed by BrdU assay. RASF were Calcein-AM stained. Cells were transferred to 24-well plates after 18h stimulation. After adhesion for 1h, non-adherent cells were removed by shaking 3x for 5 min. Afterwards, fluorescent cells were quantified. For the flow-adhesion assay, HUVEC were cultured on rattail collagen coated capillary slides. HUVEC and RASF were stimulated for 4h with TNFα, TNFα+activin A or TNFα+follistatin. After stimulation, 2x10^6 RASF were resuspended in 20ml medium and sent through the capillaries. Two 1min videos were recorded (18.4ml/h, 30.5ml/h). Settings: TNFα-stimulated RASF on HUVEC stimulated wit TNFα or activin A+TNFα or follistatin+ TNFα. For migration assays, 2% FCS medium with 1x10^5 cells were placed in inserts (8µm membrane) into wells with 10% FCS (control: 2%FCS vs. 2%FCS) and stimulated with, IL-1, IL-1+activinA and IL-1+follistatin. After 16h, migrated cells were quantified. For scratch-assay 4x10^5 cells were cultured overnight, then cells were scratched and stimulated, afterwards 3 pictures per scratch were taken at start, after 10h and every 1.5h. Cells migrating into the scratch area were quantified.Results:IL-1 induced activin A in RASF and HUVEC in all settings. IL-1-induced activin A release was reduced by follistatin in HUVEC monoculture and both cocultures compared to IL-1 alone but not in RASF monoculture. IL-1-induced IL-6 release was reduced by activin A in HUVEC and indirect coculture but not in RASF monoculture and direct coculture. Follistatin did not alter IL-6 responses. IL-1 induced VEGF in RASF but not in HUVEC and was not altered by activin A. Short-term adhesion showed no significant influence of activin A or follistatin (n=4). Flow adhesion assay showed reduced adherence/rolling of RASF on HUVEC stimulated with TNFα and activin A (n=4). Migration assays showed that IL-1 decreased migration but without significant difference between the induced effects mediated by IL-1+activinA and IL-1+follistatin (n=4). Scratch assay showed increased migration in dicrect coculture with greater difference between stimulated and unstimulated cells in RASF monoculture and indirect coculture (n=4). Proliferation was not altered by activin A or follistatin.Conclusion:In direct and indirect coculture of HUVEC with RASF the effect on HUVEC was dominant leading to reduced IL-1-induced activin A release. However, the IL-1-induced IL-6 release in RASF or HUVECs was decreased by activin A in HUVEC monoculture and indirect coculture but not during cell-contact between both cell types. The direct interaction of RASF with HUVEC seems to prevent the reducing activin A effect on IL-6 release in HUVECs. Activin A seems to not to have an impact on short-term cell adhesion but first observations show, that activin A alters selectin-mediated adhesion under flow conditions. The migration assay shows that IL-1-induced effects on cell migration were enhanced by activin A and follistatin. Migration assay shows that IL-1-induced decrease on migration more prominent in indirect coculture and RASF monoculture than in direct coculture although in gap migration in the scratch assay was highest in direct coculture.Disclosure of Interests:None declared
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Dissertations / Theses on the topic "Scratch Assay"

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Morgaenko, Katsiarina. "Sledování migrace buněk v mikrofluidním systému metodou „Scratch Wound Healing Assay“." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2019. http://www.nusl.cz/ntk/nusl-403757.

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Tato diplomová práce se zabývá popisem principů kultivace embryonálních fibroblastových buněk myší (3T3), lidských endoteliálních buněk odebraných z pupečníkové žily (HUVEC) a epiteliálních buněk vaječníku čínského křečka (CHO) v mikrofluidních systémech simulujících kapiláry. Byly provedeny literární rešerše v oblasti realizací experimentu “Scratch Wound Healing Assay” v mikrofluidních systémech s použitím fibroblastů a endotheliálních buněk. V práci jsou dále popsány principy konfokální a fluorescenční mikroskopie a metody zpracování obrazů pro sledování buněčné migrace. Experimentální nastavení pro mikrofluidní realizaci “Scratch Wound Healing Assay” s použitím trypsinu – EDTA pro vytvoření rýhy, a konfokálního mikroskopu Leica TCS SP8 X pro následující snímání pořízených dat bylo navrženo a otestováno s dostatečným počtem opakování. Vhodný algoritmus pro analýzu buněčné migrace byl napsán v programovacím prostředí Matlab. Závěrem této práce je diskuze získaných výsledků.
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Jin, Wang. "Investigating the reproducibility of in vitro cell biology assays using mathematical models." Thesis, Queensland University of Technology, 2017. https://eprints.qut.edu.au/109790/1/Wang_Jin_Thesis.pdf.

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In vitro cell biology assays are routinely used to study cancer spreading, drug design and tissue repair. However, issues associated with reproducibility are reported in literature. In this thesis we investigate the overlooked source of variability that affects the reproducibility of cell biology assays, using a combined mathematical and experimental approach. By calibrating mathematical models to experimental data, we find that the initial degree of confluence significantly affects cell motility. Following the similar approach, we identify the two-phase growth in scratch assays. We then propose a proliferation mechanism for lattice-based, random walk models, which accounts for biologically more realistic crowding effects. At last, we use a lattice-based, random walk model to mimic the passaging process and find that the passage number could significantly affect the wound closure in scratch assays.
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Johnston, Stuart T. "Mathematical models for quantifying collective cell behaviour." Thesis, Queensland University of Technology, 2017. https://eprints.qut.edu.au/109793/1/Stuart_Johnston_Thesis.pdf.

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Collective behaviour is critical to a variety of biological and ecological processes, including tumour invasion, wound healing and spreading of invasive species. This thesis investigated mathematical models of collective cell behaviour, with an aim to develop techniques for applying these models to experimental data to obtain quantitative insight from experiments, and to develop novel models that accurately incorporate cellular mechanisms. We determined various appropriate techniques to extract quantitative information about cell movement and cell proliferation, given particular experimental data. We also developed novel mathematical models that accurately describe the average behaviour of cells undergoing birth, death and movement.
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Vittadello, Sean T. "Mathematical models for cell migration and proliferation informed by visualisation of the cell cycle." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/204074/1/Sean_Vittadello_Thesis.pdf.

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Cell migration and proliferation are essential for normal physiological processes, however their misregulation contributes to pathologies including cancer. In this thesis we develop and analyse new mathematical models of cell migration and proliferation, based on new experimental studies that provide visualisation of cell cycle progression, to improve understanding of the migration and proliferation of cells. In particular, we investigate cell migration as a function of cell cycle dynamics, normally-hidden cell synchronisation in cellular assays, whether cell migration and proliferation are mutually exclusive processes, and cellular mechanisms that contribute to heterogeneous cell proliferation.
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Lawson, Brodie Alexander James. "Cell migration and proliferation on homogeneous and non-homogeneous domains : modelling on the scale of individuals and populations." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/61066/1/Brodie_Lawson_Thesis.pdf.

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Cell migration is a behaviour critical to many key biological effects, including wound healing, cancerous cell invasion and morphogenesis, the development of an organism from an embryo. However, given that each of these situations is distinctly different and cells are extremely complicated biological objects, interest lies in more basic experiments which seek to remove conflating factors and present a less complex environment within which cell migration can be experimentally examined. These include in vitro studies like the scratch assay or circle migration assay, and ex vivo studies like the colonisation of the hindgut by neural crest cells. The reduced complexity of these experiments also makes them much more enticing as problems to mathematically model, like done here. The primary goal of the mathematical models used in this thesis is to shed light on which cellular behaviours work to generate the travelling waves of invasion observed in these experiments, and to explore how variations in these behaviours can potentially predict differences in this invasive pattern which are experimentally observed when cell types or chemical environment are changed. Relevant literature has already identified the difficulty of distinguishing between these behaviours when using traditional mathematical biology techniques operating on a macroscopic scale, and so here a sophisticated individual-cell-level model, an extension of the Cellular Potts Model (CPM), is been constructed and used to model a scratch assay experiment. This model includes a novel mechanism for dealing with cell proliferations that allowed for the differing properties of quiescent and proliferative cells to be implemented into their behaviour. This model is considered both for its predictive power and used to make comparisons with the travelling waves which result in more traditional macroscopic simulations. These comparisons demonstrate a surprising amount of agreement between the two modelling frameworks, and suggest further novel modifications to the CPM that would allow it to better model cell migration. Considerations of the model’s behaviour are used to argue that the dominant effect governing cell migration (random motility or signal-driven taxis) likely depends on the sort of invasion demonstrated by cells, as easily seen by microscopic photography. Additionally, a scratch assay simulated on a non-homogeneous domain consisting of a ’fast’ and ’slow’ region is also used to further differentiate between these different potential cell motility behaviours. A heterogeneous domain is a novel situation which has not been considered mathematically in this context, nor has it been constructed experimentally to the best of the candidate’s knowledge. Thus this problem serves as a thought experiment used to test the conclusions arising from the simulations on homogeneous domains, and to suggest what might be observed should this non-homogeneous assay situation be experimentally realised. Non-intuitive cell invasion patterns are predicted for diffusely-invading cells which respond to a cell-consumed signal or nutrient, contrasted with rather expected behaviour in the case of random-motility-driven invasion. The potential experimental observation of these behaviours is demonstrated by the individual-cell-level model used in this thesis, which does agree with the PDE model in predicting these unexpected invasion patterns. In the interest of examining such a case of a non-homogeneous domain experimentally, some brief suggestion is made as to how this could be achieved.
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Ponce, Bobadilla Ana Victoria [Verfasser], and Thomas [Akademischer Betreuer] Carraro. "Mathematical Models of Cell Migration and Proliferation in Scratch Assays / Ana Victoria Ponce Bobadilla ; Betreuer: Thomas Carraro." Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1201551110/34.

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Matsiaka, Oleksii. "New mathematical models for cell biology assays incorporating realistic cell size dynamics." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/198192/1/Oleksii_Matsiaka_Thesis.pdf.

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This thesis provides novel insights into several contemporary problems in the mathematical biology involving migration of living cells. Primarily, we focus on cell motility and how dynamic changes in cell size affect collective cell migration. Additionally, this thesis investigates the importance of cellular heterogeneity and how it might affect the choice of modelling techniques we use to describe in vitro cell cultures.
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JIAN, KUAN-YING, and 簡寬穎. "Cloning,expression pattern and functional assay of zebrafish scratch gene." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/89799132745156629153.

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碩士
中山醫學大學
生化暨生物科技研究所
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Scratch gene play an important role in the regulation of neuron development. Recent study showed that downregulation of Scratch gene in mouse model impaired CNS differentiation. In present article, we want to examine whether knockdown of Scratch gene also cause the deficient in neurogenesis via zerbrafish model. According to the phylogenetic tree suggested zerbarfish scrt gene is high conserve with human and mouse counterpart. Based on PCR analysis and in situ hybridization assay we also founded that scrt gene is expressed from 36 hpf through 48 hpf, with relatively enrichment in adult zerbrafish brain. Knocking down of scrt gene by injection of anti-sense morpholino resulting in apoptosis in head at 24 hpf. In addition, there are massive apoptosis signal in eye region at 36 hpf. Moreover, disruption of scrt1b appeared more severe phenotype than scrt1a morphant. However, this effect could be restore via co-injection with scrt mRNA, implying the specificity of morpholino. Hence, these finding demonstrated a critical role of scrt gene not only in CNS but also in eye development.
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Steinhardt, Maximilian Johannes. "Einfluss des Prolyl-4-Hydroxylase-Domäne 2-Enzyms auf die Migration der myeloischen Zelllinien RAW und J774." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3E11-9.

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Nilsson, Linda. "Quality Management and process development from scratch: Improving laboratory capabilities in Assam, India." Thesis, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393548.

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During the past ten years, the foreign direct investment in India has increased with 1828 percent which has led to more projects being initiated in India to improve the livestock industry. The World Bank is the foremost entity in such investments and an issue arises when translating western theories for project management in new cultures. Because of this, development projects tend to stagnate and become inefficient since project goals and templates cannot be applied. Previous studies conducted in Africa have created successful methods called SLMTA to make projects and processes more efficient. SLMTA is a framework to improve the capability and accreditation in laboratory environment. Total Quality Management is one of the methods used in this framework. This study examines how Total Quality Management and process mapping can improve the capability of a laboratory in Guwahati, Assam, India. The underlying theories of this study derives from project management and total quality management where role definition, project triangle, project process, process management and the pareto principle are used. The study is supported by methods from total quality management such as process mapping, affinity diagram, relationship diagram, and process decision program chart. The findings from the study indicate that the development project of the laboratory in central Guwahati is shaped as a project process where project management and process development are implemented in parallel which impairs the efficiency. The study also shows cultural knowledge is a key aspect to create efficient projects and processes in development countries. Finally, the study finds that culturally adapted quality management tools, process mapping and project management are vital for improved efficiency in laboratory environment.
De senaste tio åren har internationella direktinvesteringar i Indien ökat med 1 828 procent och detta har lett till att flera olika projekt initierats i Indien för att förbättra livsmedelsindustrin. Världsbanken är ledande i dessa investeringar och en problematik uppstår när deras västerländska teoretiska ramverk för projektplanering skall översättas i nya kulturer. På grund av detta tenderar utvecklingsprojekt att stagnera och bli ineffektiva eftersom projektmål och mallar inte kan appliceras i den kultur de verkar i. Framgångsrika metoder för att effektivisera projekt och processer har tagits fram i tidigare forskning utförda i Afrika vid namn SLMTA. SLMTA är ett ramverk för att effektivisera kapabilitetens och ansvarstagandet i utvecklandet av laboratorium. En del av de metoder som används är offensiv kvalitetsutveckling. Denna studie undersöker därför hur offensiv kvalitetsutveckling och processkartläggning kan effektivisera kapabiliteten i ett laboratorium i Guwahati, Assam, Indien. Teorin som understödjer studien är tagna från projektledning och offensiv kvalitetsutveckling där roll definitioner, projekttriangeln, process projekt, processledning och pareto principen används. Studien stödjs upp genom metoder från offensiv kvalitetsutveckling som processkartläggning, släktskaps-, relations- och processbesluts diagram. Studien finner att utvecklingsprojektet av laboratoriet i centrala Guwahati är utformat som ett projektprocess där projektledning och processutveckling sker parallellt vilket försvårar effektiviteten. Studien visar även att kulturellförståelse är en avgörande aspekt för att kunna skapa effektiva projekt och processer i ett utvecklingsland. Slutligen finner studien att offensiv kvalitetsutveckling och implementeringen av kulturellt anpassade kvalitetsverktyg, processkartläggning och projektledning är avgörande för att öka effektiviteten.
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Book chapters on the topic "Scratch Assay"

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Cory, Giles. "Scratch-Wound Assay." In Methods in Molecular Biology, 25–30. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-207-6_2.

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Martinotti, Simona, and Elia Ranzato. "Scratch Wound Healing Assay." In Methods in Molecular Biology, 225–29. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/7651_2019_259.

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Zabary, Yishaia, and Assaf Zaritsky. "A MATLAB Pipeline for Spatiotemporal Quantification of Monolayer Cell Migration." In Bioimage Data Analysis Workflows ‒ Advanced Components and Methods, 175–206. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-76394-7_8.

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AbstractIn this chapter we present a MATLAB-based computational pipeline for the quantification of monolayer migration assays. Wound healing assay (or scratch assay) is a commonly used in vitro assay to assess collective cell migration. Our pipeline outputs traditional and spatiotemporal readouts that quantify the group migration properties and was previously used for a screen that included thousands of time-lapse sequences. You will learn how to execute the pipeline, the principles behind the design and implementation choices we made, pitfalls, tips, and tricks in using it.
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Vašinková, Markéta, Michal Krumnikl, Zuzana Mikulková, Petr Gajdoš, and Eva Kriegová. "Simple Approach for Dynamics Evaluation of Scratch Wound Healing Assay." In Advances in Intelligent Networking and Collaborative Systems, 380–92. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-14627-5_39.

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Glaß, Markus, Birgit Möller, Anne Zirkel, Kristin Wächter, Stefan Hüttelmaier, and Stefan Posch. "Scratch Assay Analysis with Topology-Preserving Level Sets and Texture Measures." In Pattern Recognition and Image Analysis, 100–108. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-21257-4_13.

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Wiegand, C., J. Tittelbach, U. C. Hipler, and P. Elsner. "Water-Filtered Infrared A Irradiation: From Observations in Clinical Studies to Complex In Vitro Models." In Water-filtered Infrared A (wIRA) Irradiation, 203–12. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-92880-3_17.

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AbstractSuccessful treatment of recalcitrant common hand and foot warts in a prospective randomized controlled blind trial using wIRA and PDT has been reported. In addition, in wound healing wIRA is mostly investigated in vitro based on the resolution of mechanical damage to confluent cell layers using the “scratch wound assay.” The latter enables the direct measurement of cell migration and regeneration of the cell layer. Preliminary studies for wIRA effects on wound closure in vitro have shown beneficial effects of single 10 min treatments. Although cellular processes induced and mediators involved still need to be elucidated, it is apparent that the observed clinical benefits of wIRA on wound healing can be investigated in vitro using adequate models and experimental settings. The next step is to employ 3D skin models for morphological investigations closely simulating in vivo conditions.
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Castellano-Pellicena, Irene, and M. Julie Thornton. "Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration." In Methods in Molecular Biology, 1–12. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0648-3_1.

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Kobelt, Dennis, Wolfgang Walther, and Ulrike S. Stein. "Real-Time Cell Migration Monitoring to Analyze Drug Synergism in the Scratch Assay Using the IncuCyte System." In Methods in Molecular Biology, 133–42. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1350-4_9.

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"Scratch Wound Assays." In Biomedical Image Analysis Recipes in MATLAB®, 135–213. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118657546.ch5.

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Virador, Gabriel, Lisa Patel, Matthew Allen, Spencer Adkins, Miguel Virador, Derek Chen, Win Thant, Niloofar Tehrani, and Victoria Virador. "Systematically Assessing Natural Compounds’ Wound Healing Potential with Spheroid and Scratch Assays." In Advances in Experimental Medicine and Biology. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/5584_2022_727.

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Conference papers on the topic "Scratch Assay"

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Caramazza, Laura, Annalisa De Angelis, Daniel Remondini, Gastone Castellani, Micaela Liberti, Francesca Apollonio, and Isabella Zironi. "Galvanotactic Phenomenon Induced by Non-Contact Electrostatic Field: Investigation in a Scratch Assay*." In 2020 42nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) in conjunction with the 43rd Annual Conference of the Canadian Medical and Biological Engineering Society. IEEE, 2020. http://dx.doi.org/10.1109/embc44109.2020.9175695.

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Erdamar, Aykut, Sila Yilmaz, Tansel Uyar, and Ozlem Darcansoy Iseri. "A new image segmentation method for quantitative analysis of in vitro scratch assay." In 2017 25th Signal Processing and Communications Applications Conference (SIU). IEEE, 2017. http://dx.doi.org/10.1109/siu.2017.7960399.

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Li, Xingyu, and Konstantinos N. Plataniotis. "Computational scratch assay — A new frontier for image analysis: Preliminary study of multi-cellular segmentation." In 2017 IEEE Global Conference on Signal and Information Processing (GlobalSIP). IEEE, 2017. http://dx.doi.org/10.1109/globalsip.2017.8308629.

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Zhu, Longjing, Stacie A. Chvatal, Heather B. Hayes, Daniel C. Millard, and Cheryl T. Gomillion. "Abstract 4918: Modified citrus pectin slows migration of triple negative breast cancer cells in an impedance-based scratch assay." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-4918.

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Torrissen, Martina, Astrid Nilsson, Binh Minh Trinh, Elisabeth Ytteborg, Gerd Marit Berge, Harald Svenson, Iren Stoknes, and Marta Bou Mira. "Novel n-3 very-long-chain polyunsaturated fatty acids and their potential role in skin tissue." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/nkdk5807.

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Very-long-chain fatty acids (VLC-FA) have a chain length of ≥24 carbon atoms. They are generally not provided through dietary sources but generated endogenously, involving chain elongation of LC-FA by ELOVL4. There is emerging substantial evidence suggesting they play important roles in tissues where they are naturally found, including retina, skin, testis, and brain. Mutations in ELOVL4 have been associated with several tissue-specific conditions, suggesting these FA may be involved in the disease pathology. A lack of availability of these fatty acids for dietary interventions has however, until recently, made them difficult to investigate. After identifying VLC-FA in fish oil and developing a method for concentrating n-3 VLC-PUFAs in kg scale, our research team have conducted feeding trials to determine if they are taken up directly from diet through supplementation, and their effect on development and maturation of skin tissue. Salmon fed different dietary levels of the concentrate were analysed for tissue fatty acid composition by GC and histology by H&E and Von Kossa staining. After establishing a clear tissue-specific uptake, we conducted in-vitro trials where we observed promising effects by incubating skin cells from human and Atlantic salmon with n-3 VLC-PUFA concentrate in scratch assay and cell migration trials. The in-vitro results show improved cell migration, which is in line with our in-vivo findings and demonstrates a promising effect on skin tissue development, maturation, and skin cell migration. Here we will present our data and discuss the relevance of this in skin biology. As VLC-FA potentially play a critical role in skin barrier function and skin biology, understanding these FAs may lead to improvements in treatment of dermatological diseases and conditions.
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