Academic literature on the topic 'Seed dormancy. eng'

Freshly fallen fruits of T. calycina contained seeds which were completely dormant; none germinated after 200 days at 20°C. Seeds excised with testas intact from fresh fruits were partially dormant; one-third germinated after 60 days. The dormancy of seeds in freshly fallen fruits was imposed jointly by the fruit and the seed. The major site of the dormancy was however the seed coat since tearing part of it away from seeds excised from fresh fruits resulted in rapid and complete germination. Fruits stored dry in a laboratory at 20°C for 90 days were partially dormant. Nicking the distal end of these fruits enhanced germination. Seeds excised from these laboratory stored fruits had 85 % germination, which indicated a reduction in the seed imposed dormancy. Germination of T. calycina was independent of light and, although the fruits contained large amounts of phenolic material this did not inhibit germination. Fruits weathered in the field for at least 2 years contained less viable seeds, presumably because of insect predation, but these all germinated within 50 days at 20°C. Brief washing of fruits in concentrated sulphuric acid increased germination. Germination was not enhanced by treatment with low concentrations of gibberellic acid in the presence or absence of cytokinin.

AbstractWe investigated seed dormancy among species of Melastomataceae from Neotropical montane vegetation of Brazil. Four out of 50 studied species had dormant seeds:Miconia corallina(Miconieae), Tibouchina cardinalis(Melastomeae), Comolia sertularia(Melastomeae) andChaetostoma armatum(Microlicieae). For these four species, germinability of seeds collected in different years was always < 10% and the percentages of embryoless seeds and non-viable embryos were both insufficient to explain low or null germinability. This is the first unequivocal report of seed dormancy in tropical Melastomataceae. The production of seeds with permeable seed coats and fully developed, differentiated embryos indicates the occurrence of physiological dormancy. The reconstructed phylogenetic tree of the 50 species suggests that physiological dormancy evolved multiple times during the evolutionary history of Melastomataceae in this vegetation. Physiological dormancy evolved in species and populations associated with xeric microhabitats, where seeds are dispersed in unfavourable conditions for establishment. Therefore, drought-induced mortality may have been a strong selective pressure favouring the evolution of physiological dormancy in Melastomataceae. We argue that dormancy may have been independently selected in other lineages of Cerrado plants colonizing xeric microhabitats and dispersing seeds at the end of the rainy season. The contributions of our data to the understanding of seed dormancy in tropical montane vegetation are discussed.

Partially after-ripened seeds of three dormoat lines (Avena sativa L. × A. fatua L.) were treated to induce secondary dormancy before field planting in the fall. The objectives of this experiment were to evaluate the dormancy response of the treated seeds and to determine if field performance, in terms of both survival and emergence, was improved by the treatments. Treatments consisted of imbibition and incubation of seeds at two temperatures: 4 °C for 3 wk or 30 °C for 2 wk. Both treatments increased dormancy, but differences were also noted in the type of relative dormancy expressed by each seed population under laboratory conditions. Treated seeds and the appropriate untreated control seeds were sown in the field, in fiberglass mesh bags, either in early or late fall depending on the treatment. Levels of dormant seeds retrieved from the field, at the end of the fall season, varied for each dormoat line and did not reflect the induced secondary dormancy measured before field planting. The fate of these dormant seeds after exposure to winter stresses was assessed on field-retrieved material in the spring. In relation to a late sowing of untreated seeds, the treatments were effective in improving levels of viable seeds (dormant or germinating) remaining in the ground, in the spring. However, the best performance, both in survival and emergence, was obtained with an early fall planting of untreated seeds. High loss of viability was common to both treated and untreated seeds of the three dormoat lines. The major shortcoming of dormoats seems to be in the area of cold resistance of the fall dormant seeds since damage during winter played an important role in survival.Key words: Secondary dormancy, relative dormancy, dormoats, emergence

AbstractMany research questions focused upon seed dormancy and germination remain unanswered due to problems created by inappropriate assumptions concerning the design and implementation of experiments. Lack of attention to the genetic background and growing conditions required for seed production and subsequent storage may compromise an investigator's ability to pool data or assimilate data trends due to variability in seed performance from year to year. Seed aging events during the afterripening process can be mistaken for those involved in the transition from the dormant to nondormant state. After imbibition, inaccurate criteria for and scoring of the germination event may lead to confusion of seed vs. seedling physiology and biochemistry. In addition, a sufficient number and proper spacing of data points during timecourse experiments are required to properly stage a sequence of events. Studies comparing dormant vs. nondormant seeds must be supplemented with kinetic experiments examining the effect of a dormancy-breaking treatment upon the transition between the dormant and nondormant states. In comparisons of the relative efficacy of various dormancy-breaking chemical treatments, the physical properties of substances under evaluation (e.g. lipophilicity) should be considered. In addition, since many dormancy-breaking chemicals are toxic to vegetative growth, there is a danger of introducing pharmacological artifacts that confuse interpretation of the results obtained. These toxic properties also necessitate the use of viability tests during construction of dose-response curves to avoid confusing seed death with dormancy. Seed scientists must also avail themselves of the extensive literature on developmental arrest in other organisms, which may generate fresh ideas for experimentation.

The seed dormancy characteristics of 2 capeweed [Arctotheca calendula (L.) Levyns] ecotypes from Western Australia were studied to determine aspects of seed dormancy that contribute to the success of this species in southern Australia. Short- and long-term dormancy pattern of buried and soil surface seed, effect of summer temperatures on afterripening, and effect of temperature on seed germination were investigated using seed produced in a common environment. There were large differences in the seed dormancy pattern of the 2 ecotypes studied. On the soil surface, >95% of seed of the Mt Barker ecotype became non-dormant and germinated in the first year, the remainder germinating the following season. In contrast, only 5% of Mullewa seed germinated in the first year, with 75% germinating in the second year and 20% of seed remaining dormant after 2 years. Cycling of dormancy was observed for buried seed of both ecotypes, with periods of non-dormancy corresponding with the likely timing of the break of the season. Dormancy cycling was also apparent in seed stored under constant conditions in the laboratory. Burial prevented germination of both ecotypes; however, the ability to resist germination while buried was lost in 30% of the Mt Barker seed in the second season. Differences in the duration of dormancy of soil surface and buried capeweed seed have evolved as an adaptation to the different environments likely to be experienced by plants at their site of collection. All seeds possessed primary dormancy at maturity, with any afterripening during the first year occurring by the end of summer. Afterripening was enhanced by exposure to typical soil surface temperatures, providing some protection against germination during early summer rainfall. Protection from late summer rains is insured by the inability of seed to germinate at temperatures >30°C and a relatively slow rate of germination. These features of capeweed seed dormancy, combined with the ability to evolve genetically distinct populations suited to particular environments, help explain why capeweed is so widespread and abundant across southern Australia.

Large numbers of viable, diffuse (Centaurea diffusa Lam.) and spotted knapweed (C. maculosa Lam.) seeds (achenes), collected in the interior of British Columbia, failed to germinate in darkness at 25 °C. This primary dormancy was released to varying degrees by gibberellic acid, exposure to red light, or excision of the distal end of the seed. The effect of red light was negated by subsequent exposure to far-red light. The demonstration of red/far-red reversibility implicates the phytochrome pigment system in the light-sensitive germination of knapweed seeds. Seeds collected from different sites, and from individual plants within sites, had different germination levels in darkness and following exposure to 2 min of red light. Three types of germination behavior were evident: nondormant seeds germinated in darkness; light-sensitive dormant seeds germinated in response to red light; and light-insensitive dormant seeds failed to germinate after 5 d of continuous red light. Seeds of all three germination types were found on individual plants.Key words: Centaurea diffusa, Centaurea maculosa, knapweed, seed dormancy, light-sensitive germination, germination polymorphism

Pre-harvest sprouting (PHS) damage leads to occasional massive losses in all wheat producing areas, causing downgrading of grain quality, that severely limits end-use applications and results in substantial financial losses to farmers and food processors. Red grain color is a traditional marker for resistance to sprouting in wheat breeding programs, however red-grained genotype alone does not always guarantee effective resistance. The objective of this work was to find genes for resistance to PHS and investigate its inheritance in Brazilian wheat cultivars. Genetic variation for dormancy was investigated in the parents, F1 and 300 F2 lines derived from the cross Frontana × OR1 and its reciprocal. The germination/dormancy sprouted grains was evaluated on fifty seeds per replication, germinated in paper towel rolls at 20ºC for 5 days. A bimodal distribution for dormancy occurred in the Frontana/OR1 and OR1/Frontana derived F2 populations. The mean ratio of dormant and non-dormant seeds of the cross and its reciprocal was 85:1115, fitting a digenic model of 1:15 (P < 0.05). In fact, all non after-ripened F1 seeds germinated. The F2 distribution indicates that two major genes, here called A,a and B,b, control seed dormancy, which it appears to be recessive to dormancy. Only the homozygous aabb is dormant. As expected, there was no effect of maternal tissues.

Light is one of the most important environmental factors regulating seed germination. It is known that light inhibits seed germination of some monocotyledonous species and that it is mostly related to the blue wavelength of the spectrum received by cryptochromes (cry). Research has also found that the red light (R) stimulates germination of dicotyledonous seeds and that this reaction involves mainly phytochromes (phy). Surprisingly, up to date, the role and the mechanism of action of blue light (BL) in seed biology of dicot plants is still very poorly understood and some questions are unexplained, e.g., whether BL plays a role in regulation of dicot seeds dormancy and/or germination? If, so what particular elements of light signaling pathway are involved in modulation of this(ese) process(es)? Also, is the BL action in regulation of dicot seeds dormancy and/or germination maybe due to changes of expression of genes related to metabolism and/or signaling of two phytohormones controlling seed-related events, such as gibberellins (GA) and abscisic acid (ABA)? To answer these intriguing questions, the combination of biological, transcriptomic, and genetic approaches was performed in this particular study. The germination tests show that freshly harvested wild type (WT) Arabidopsis thaliana Col-0 seeds are dormant and do not germinate in darkness (at 25 °C), while nondormant (after-ripened) seeds germinate well in these conditions. It is also proven that dormancy of seeds of this species is released in the presence of white and/or BL (λ = 447 nm) when placed at 25 °C. Presented here, novel results emphasize the role of BL in dormancy alleviation of dicot seeds, indicating that this wavelength of light spectrum received by phyB induces this process and that the sensitivity to this stimulus depends on the depth of seed dormancy. In addition, it is demonstrated that various elements of phy-mediated pathway can be used in response to the signal induced by BL in germinating dormant seeds of Arabidopsis. The quantitative real time PCR analysis supported by results of germination tests of WT, T-DNA insertion mutants (i.e., hy5, hfr1, and laf1) and overexpression transformants of Arabidopsis seeds (i.e., 35S:OE:HY5, 35S:OE:HYH, 35S:OE:HFR1, and 35S:OE:LAF1) revealed that the HY5 gene coding transcription factor is most probably responsible for the control of expression of genes involved in GA/ABA metabolism and/or signaling pathways during BL-dependent dormancy alleviation of Arabidopsis seeds, while biological functions of HYH and HFR1 are associated with regulation of germination. The model of BL action in regulation of dormancy alleviation and germination potential of Arabidopsis seeds is proposed.

AbstractCapeweed (Arctotheca calendula) seeds were found to be dormant at harvest. Effects of duration and temperature of storage under ‘laboratory’ and ‘natural’ conditions, growth regulators, stratification and age of seeds, were studied on the germination of dormant seeds. Three factors imposed on seeds were found to promote germination of capeweed: (i) allowing seed to age, either in storage, buried in soil or during stratification (germination of 18-month-old seeds was up to 60% higher than that of fresh seeds); (ii) the presence of light (the average germination percentage of seeds exposed to light during storage was 3.2-fold greater than that of seed stored in the dark); and (iii) the application of growth regulators, particularly gibberellic acid, enhanced by scarification (GA3and ethephon promoted germination by up to 58% when applied to scarified seeds). Other factors, e.g. temperature and depth of storage, influenced germination but were less critical to its success. These results provide a preliminary assessment of the importance of these factors in controlling dormancy in seeds of capeweed.

Seed yield and overwintering of seeds of 22 Australian subterranean clover varieties were investigated in 1986 and 1987 at the Viikki Experimental Farm of the University of Helsinki. The seed yield varied remarkbly according to the variety and weather conditions of the two summers. Some varieties, such as Seaton Park, Woogenellup and Karridale, and during the summer of 1987 also the early variety Dalkeith, produced lots of seed. Some late varieties, e.g. Treeton, Tallarook and Esperance, produced very few seeds or no seeds at all. A considerable proportion of the seeds degenerated during the wet autumn conditions, by the end of October. Many seeds showed embryo dormancy, but complete dormancy or hard seeds were found only exeptionally. In spring 1988, some seedlings which had developed from overwintered burrs were found. There seems to be little hope of finding self-seeding subterranean clovers suitable for Finnish growing conditions.

Orientador: Teresinha de Jesus Deléo Rodrigues
Banca: Maria Lidia Stipp Paterniani
Banca: Ana Regina Pimentel de Almeida
Resumo: Com o objetivo de estudar a dormência tegumentar e caracterizar possíveis relações entre tamanho e qualidade fisiológica e permeabilidade dos tegumentos, qualidade fisiológica e permeabilidade dos tegumentos, teor de água inicial e qualidade fisiológica de sementes de mucuna preta, foram conduzidos os seguintes experimentos: determinação do teor inicial de água dos lotes (RAS); DIC, 4 repetições de 25 sementes cada; peso de 100 sementes, 4x100, DIC; teste de %G e IVG 4x25 cada, DIC; 12 tratamentos para superação de dormência; classificação dos lotes em três classes de tamanho; teor inicial de água das sementes de diferentes tamanhos (RAS), DBC, repetições 4x25, fatorial 6 lotes x 3 tamanhos; curvas de embebição com dados em dispersão e uso de função polinomial de quarto grau, dados obtidos pela razão peso final(PF)/peso inicial(PI); teste de CE com repetições 4x25, DIC, fatorial 6 lotes x 3 tamanhos, substrato solo/areis. Os lotes apresentaram relação positiva inversa para teor de água inicial em relação à germinação; resultados dos tratamentos para superação de dormência com interações para lotes e para tamanhos dentro dos lotes; os testes demonstraram haver maior interação entre lotes, ocorrendo o contrário para a variável tamanho de sementes. Concluiu-se que: sementes pequenas são mais permeáveis em lotes não dormentes; entre lotes de germinações semelhantes o de menor teor de água inicial é o de maior vigor; sementes grandes produzem mais fitomassa e o tratamento mais eficiente para quebra de dormência foi escarificação com ácido sulfúrico concentrado (98%) por 7 minutos.
Abstract: Aiming to study damage and characterize possible relationships between size and physiological quality; size and coat permeability; physiological quality and tegument permeability; initial content of water and physiological quality of mucuna preta sedes (Stizolobium aterrimum Pierce & Tracy) the following experiments were conducted: análisis of water content of seed lots by oven meted at 105°C during 24 hours using with four replications of 100 seeds each; germination test (%G) and speed of germination index (SGI) with four replications of 25 seeds in complete randomized design; twelve treatments to break dormancy The seed lots presented inverse positive relationship for water content related to germination; treatment to break dormancy with interaction between lotsand seed sizes within lots; the tests demonstrated higher interaction between lots, occuring the inverse for the variable seed size; the imbibition curve proved to be na important tool in studies related with tegument permeabillity and levels of vigor among lots. The following conclusion can be withdrawn form data: small sedes are more permeable in nondormant lots; seed lots with similar germination or with lower water content, higher is the vigor; large sedes produce higher amount of phytomass. The best treatment to break seed dormancy of mucuna preta seed was acid escarification during seven minutes.
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Orientador: Cibele Chalita Martins
Banca: João Nakagawa
Banca: Ciniro Costa
Resumo: O tempo de 28 dias recomendado pelas Regras para Análise de Sementes (RAS) para o teste de germinação de sementes de Panicum maximum é considerado excessivo para produtores, comerciantes e laboratórios de análise de sementes forrageiras, que dependem dos resultados do teste para a tomada de decisões de controle de qualidade e comercialização. Desta forma, o presente trabalho teve o objetivo de determinar o tempo necessário para a condução do teste de germinação de Panicum maximun cv. Tanzânia. Na pesquisa, 30 lotes de sementes fiscalizadas foram submetidas ou não (testemunha) aos seguintes tratamentos para a superação dormência: germinação em substrato umedecido com KNO3 (0,2%), escarificação com ácido sulfúrico (98% 36N) por 5 minutos e testemunha. Os lotes foram avaliados pelo teste de germinação sob duas condições de temperaturas alternadas (20-30ºC e 15-35ºC), com luz (78 μmol s-1 m-2/ 8h). Para a identificação da data de término do teste foram realizadas contagens diárias do número de plântulas normais e para cada lote, tratamento de superação de dormência e temperatura, foi ajustada uma curva de crescimento para a avaliação da germinação. As sementes remanescentes do teste de germinação foram seccionadas longitudinalmente e medianamente através do embrião para a realização do teste de tetrazólio. As duas metades da semente foram imersas em uma solução de tetrazólio a 0,1% e mantidas em câmara escura, à 37ºC, por um período de 3 horas e após esse período as sementes foram lavadas e a leitura feita imediatamente, classificando-se as sementes em viáveis e não viáveis (mortas). No delineamento experimental os 30 lotes foram considerados... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The time of 28 days recommended by the Rules for Seed Analysis (RAS) to test the germination of Panicum maximum is considered excessive for producers, traders and laboratory analysis of forage seeds, which depend on the results of the test for making decisions of quality control and marketing. The present study aimed to determine the time required for the conduct of the germination test of Panicum maximum cv. Tanzania. In the survey, 30 seed lots were audited or not (control) to the following treatments to overcome dormancy: germination in soak with KNO3 (0.2%), scarification with sulfuric acid (98% 36N) for 5 minutes and witness. The lots were assessed by the germination test under two conditions of alternating temperatures (20-30ºC and 15-35°C) with light (78 μmol s-1 m-2/ 8h). To identify the date of termination of the test were made daily counts of the number of normal seedlings for each lot, treatment of overcoming dormancy and temperature, was fitted a curve of growth for the assessment of germination. The remaining seeds in the germination test were sectioned longitudinally through the embryo and medium for the completion of the tetrazolium test. The two halves of the seeds were immersed in a solution of tetrazolium to 0.1% and kept in dark room, at 37º C for a period of 3 hours and after that period the seeds were washed and reading made immediately, sorting out the seeds into viable and non viable (dead). In the experimental design the 30 lots were considered the repetitions, resulting in estimates of the parameters of the segmented regression model for each treatment, was performed an analysis of variance where the treatments were compared to overcome dormancy and temperature. Through regression model was targeted to estimate the time required to conduct the germination test, determining whether the value at which the difference between the estimated asymptote... (Complete abstract click electronic access below)
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Resumo: O tempo recomendado pelas Regras para Análise de Sementes (R.A.S.), para a conclusão do teste de germinação de Brachiaria brizantha, é considerado longo pelos laboratórios de análise, produtores e comerciantes dessas sementes, representando um entrave na tomada de decisões relacionadas à comercialização. O presente trabalho objetivou determinar o tempo mínimo necessário à realização do teste de germinação para sementes de B. brizantha cv. Marandu (Hochst. ex A. Rich.) Stapf e determinar o método de superação de dormência e a temperatura, recomendados pelas R.A.S., que proporcionem a maior germinação no menor tempo. A pesquisa foi conduzida em duas etapas. Na primeira, trinta lotes de sementes foram submetidos ao teste de germinação, com quatro repetições, sob duas condições de temperaturas alternadas (15-35ºC e 20-35ºC), sem (ausente) e com os seguintes métodos para a superação de dormência: umedecimento do substrato com KNO3 (0,2%) e imersão das sementes em H2SO4 (98% 36N) por 15 minutos, seguido de lavagem em água, constituindo 6 tratamentos. Realizaram-se contagens diárias do número de plântulas normais, para a determinação da data mais apropriada para o término do teste. Na segunda etapa foram realizados testes de germinação em oito lotes, sendo dois de alto vigor, quatro de médio vigor e dois de baixo vigor, nos mesmos 6 tratamentos e com encerramento nas datas definidas na primeira etapa da pesquisa. As sementes não germinadas após o término do teste de germinação foram submetidas ao teste de tetrazólio para verificar sua vitalidade, e, dessa forma, comprovar a eficiência da metodologia estabelecida na primeira etapa. O delineamento experimental considerou os lotes como repetições. Na primeira etapa para cada repetição, em cada combinação de tratamento, foi realizado um ajuste... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The time recommended by the Brazilian Rules for Seed Analysis (R.A.S.) for the ending of the Brachiaria brizantha germination test, is considered long for the analysis laboratories, producers and merchants of those seeds, representing a problem in taking decisions related to commercialization. The present work had as objective to define the minimum time necessary to carry out the B. brizantha cv. Marandu (Hochst. ex. A. Rich.) Stapf. germination test and determine the dormancy breaking method and the temperature, recommended by R.A.S, that provide the largest germination in the smallest period. The research was carried out in two parts. In the first, 30 lots of seeds were submitted to the germination test, with four replicates, under two conditions of alternated temperatures (15-35ºC and 20-35ºC), without (none) and with the following dormancy breaking methods: substrate moistened with a solution of KNO3 (0,2%) and immersion of the seeds in H2SO4 (98% 36N) for 15 minutes, followed by a wash in water, forming 6 treatments. Daily counting of the number of normal seedlings was carried out, to define the most adequate date to the end of the test. In the second part germination tests were carried out in eight lots, being two of high vigor, four of medium vigor and two of low vigor, in the same 6 treatments and with the ending in date indicated in the first part of the experiment. The remaining seeds at the end of the germination test were submitted to the tetrazolium test to verify their viability, and, thus, confirm the efficiency of the methodology defined in the first part. The experimental design considered the lots as replicates. In the first part for each replicate, in each treatment combination, a non-linear adjustment was accomplished, in which the function parameters were estimated, and a growth curve to determine the minimum time necessary to carry out... (Complete abstract, click electronic access below)
Orientador: Cibele Chalita Martins
Coorientador: Cláudio Cavariani
Banca: Joao Nakagawa
Banca: Adriana Martinelli Seneme
Mestre

L'éthylène comme la stratification au froid et le GA3, stimule la germination des graines d'Arabidopsis thaliana (Col-0) ayant une dormance primaire, à 25 °C et à l'obscurité. L'élimination de la dormance au froid n'exige pas ETR1, EIN4 et EIN2, alors que l'effet du GA3 nécessite ETR1. L'effet stimulateur de l'éthylène est associé à une réduction de l'expression des gènes impliqués dans les voies de signalisation des GAs (DELLAs) et de l'ABA (ABI5). Les graines du mutant prt6 affectées dans la voie "N-end rule" de la protéolyse, sont insensibles à l'éthylène, montrant que PRT6 intervient dans l'action de l'éthylène, et cette insensibilité résulte aussi d'une interrelation avec la balance ABA/GAs. D'autre part, les facteurs de transcription de la réponse à l'éthylène du groupe VII, (ERF VIIs), substrats de la voie "N-end rule", interviennent dans cette insensibilité. La sensibilité à l'éthylène de prt6rap2.2rap2.3rap2.12 et l'insensibilité de prt6hre1hre2, permet de montrer que les 3 RAPs et les 2 HREs interviennent respectivement en aval et en amont de PRT6. L'éthylène induit une diminution de l'expression des 3 RAPs dans Col, mais maintient ou induit celle-ci dans prt6. De plus, l'expession de HRE2 augmente dans prt6, mais diminue dans prt6rap2.2rap2.3rap2.12, suggérant que les 3 RAPs peuvent réguler l'expression de HRE2. De plus, l'éthylène modifie différentiellement le protéome de Col et de prt6 avec respectivement 587 et 30 protéines significatives. L'analyse des classes fonctionnelles a permis d'identifier la réponse à hypoxie comme processus biologique spécifique de prt6, mais leur insensibilité à l'éthylène est indépendante des ROS et de l'intensité respiratoire
Ethylene as chilling and GA3, was able to improve the germination of primary dormant seeds of Arabidopsis thaliana (Col-0) at 25 °C in darkness. Chilling did not require EIN4, ETR1 and EIN2 involved in ethylene signaling to break seed dormancy while GA required ETR1.The improving effect of ethylene in seed germination is EIN4 independent, and is associated with a decrease in ABA/GA ratio and a down-regulation of DELLAs and ABI5 genes related to GA and ABA signaling, respectively. The mutant affected in the proteolytic N-end rule pathway, prt6, was insensitive to ethylene in seed germination evidenced that PRT6 was involved in dormancy release by ethylene, and this insensitivity was related to a crosstalk with ABA/GAs. The substrates of the N-end rule pathway, ERFVIIs (HRE1, HRE2, RAP2.2, RAP2.3, and RAP2.12), might result in the insensitivity with an increased germination in prt6rap2.2rap2.3rap2.12 rather than in prt6hre1hre2, which also indicated that the 3 RAPs acted downstream of PRT6, while the 2 HREs acted upstream of PRT6. Ethylene reduced the transcript expression of the 3 RAPs in Col-0, but the 3 RAPs were maintained or induced by ethylene in prt6. Besides, HRE2 was up-regulated in prt6 seeds, but it was down-regulated in prt6rap2.2rap2.3rap2.12, suggesting that the 3 RAPs might stimulate the expression of HRE2. Ethylene differently changed the seed proteome of Col and prt6 with 587 and 30 significant proteins, respectively. The functional class scoring analysis identified one biological process, response to hypoxia, which was distinct in prt6, however the insensitivity of prt6 to ethylene was independent of ROS production or respiration intensity

Resumo: O presente trabalho objetivou estudar aspectos relativos ao processo germinativo e à qualidade fisiológica de sementes de Piptadenia moniliformis Benth. (angico-de-bezerro). Foram conduzidos cinco experimentos: experimento 1 - visou a superação da dormência das sementes pela aplicação de diferentes tratamentos prégerminativos: imersão em água a 70°C, 80°C, 90°C e em água fervente durante 1, 2, 3, 4 e 5 minutos e imersão em ácido sulfúrico durante 1, 5, 10, 15, 20, 25 e 30 minutos e o controle; experimento 2 - visou determinar a temperatura e substrato adequados para a germinação, avaliando-se três temperaturas (25°C, 20-30°C e 20-35°C) e sete substratos (entre e sobre areia, entre e sobre papel, entre e sobre vermiculita, e o rolo de papel); experimento 3 - objetivou determinar a viabilidade de quatro lotes de sementes pelo teste de tetrazólio, submetendo-se as sementes após embebição entre papel toalha durante 24 h a 25ºC e posterior retirada do tegumento, às concentrações de 0,05%, 0,075% e 0,1% do sal 2, 3, 5 trifenil cloreto de tetrazólio, por duas, três e quatro horas de coloração, a 35°C; experimento 4 - visou a avaliação da qualidade fisiológica de 10 sub-lotes de sementes classificados quanto à coloração (clara e escura) e ao tamanho (sementes retidas em peneiras 11, 12, 13, 14 e 15), pelo teste de envelhecimento acelerado (EA) conduzido a 45°C, durante 0, 24, 48, 72, 96 e 120 h; e experimento 5 - avaliou-se a germinação de três lotes de sementes submetidos a diferentes potenciais hídricos (0, -0,3; -0,6; -0,9; -1,2 e -1,5 MPa) simulados com polietilenoglicol (PEG 6000), sob as temperaturas de 25 e 30°C. Em todos os ensaios, foram utilizadas quatro repetições de 25 sementes para cada tratamento e as leituras foram efetuadas diariamente durante um período de 21 dias. Os dados foram... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: This study aimed to evaluate aspects concerning to germination process and physiological quality of Piptadenia moniliformis Benth. seeds. Five experiments were carried out: experiment 1 - aimed to overcome the dormancy of seeds by application of different pre-germinative treatments: immersion in water at 70°C, 80° C, 90°C and in boiling water during 1, 2, 3, 4 and 5 minutes and in sulfuric acid during 1, 5, 10, 15, 20, 25 and 30 minutes and the control; experiment 2 - aimed to determine the substrate and temperature suitable for the germination tests: three temperatures (25°C , 20-30°C and 20-35°C) and seven substrates (among sand, on sand, among paper, on paper, among vermiculite, on vermiculite and the rolled paper); experiment 3 - aimed to determine the viability of four seed lots by the tetrazolium test: the seeds were soaked in paper towels during 24 hours at 25°C with subsequent removal of seed coat submitted to color at concentrations of 0.05%, 0.075% and 0.1% of salt 2, 3, 5 triphenyl chloride terazolium during 2, 3 and 4 hours at 35 ° C; experiment 4 - it was evaluate the physiological quality of 10 seeds lots classified according to color (light and dark) and size (seeds retained in sieves 11, 12, 13, 14 and 15), by the accelerated aging test (EA) conducted at 45°C during 0, 24, 48, 72, 96 and 120 hours; and experiment 5 - whose purpose was to evaluate the germination of three lots of seeds under different water potentials (0, -0.3, -0.6, -0.9, -1.2 and -1.5 MPa) simulated with polyethylene glycol (PEG 6000) in two temperatures (25 and 30°C). In all tests, four replicates of 25 seeds were used for each treatment and the germination tests were carried out during a period of 21 days. The data were submitted to variance in completely randomized design, and when appropriate in a factorial scheme, followed by test of... (Complete abstract click electronic access below)
Orientador: Sérgio Valiengo Valeri
Coorientador: Rinaldo Cesar de Paula
Banca: Sérgio Roberto Garcia dos Santos
Banca: Sonia Cristina Juliano Gualtieri de Andrade Perez
Banca: Ivor Bergemann de Aguiar
Banca: Fabíola Vitti Moro
Doutor

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The Balfourodendron riedelianum of the family Rutaceae is a native forest species with timber value and is used in the manufacture of luxury furniture, construction, and so forth. Moreover, the species is suitable for medical use, insecticide, landscaping and recovery riparian areas. Despite the importance of this species, there are few studies in relation to seed technology, irrigation in the production of seedlings and field early growth. In this sense, species diaspores were collected in Palma Nova (RS), in the years of 2013 and 2015, and further processed and divided into samples for testing. The study was divided into three articles, in Article I – measurement of biometrics, pre-germination treatments and substrates for the germination of B. riedelianum diaspores; by using a sample of 2013 for physical characterization, through the weight of a thousand seeds (PMS), water content (TU) and biometrics, besides the germination test (G%) on different substrates after breaking dormancy. The dimensions of the B. riedelianum seeds had on average, 18.59; 9.03 and 9.38 mm in length; width and thickness, respectively. The dormancy was broken by immersion in cold water (8 ± 2 ° C) for 48 hours and germinating was conducted on vermiculite substrate. Article II – In the first, two experiments were carried out, the quality of the seeds collected in 2013, which have been stored for two years in cold and humid chamber was evaluated and sampled for TU testing, G% and sanity, and the second evaluated in the lot collected in 2015 the disinfestation of the seeds in the incidence of fungi and germination. Diaspores of B. riedelianum remained viable for 12 months under storage conditions, and disinfection with Orthocide® is indicated in order that provided high G% and IVG. The genus Aspergillus sp., Fusarium sp., Cladosporium sp. and Penicillium sp. were detected in both experiments. Article III - identified water depth required to optimize the growth of seedlings of B. riedelianum, and evaluated its initial field growth. Combinations were used of heads between 4, 8 and 12 mm day-1, after 180 days of water management application survival evaluation were performed, height, stem diameter, dry mass air, root dry weight and quality index Dickson. The treatments described in the vivarium were conducted in the field, wich 450 days after plantating were evaluated in relation to H, DC, MSA, leaf area and root length, chlorophyll content and chlorophyll a fluorescence. The head influenced the growth of seedlings of B. riedelianum, in the vivarium stage, which was confirmed in the field. Seedlings of Balfourodendron riedelianum can be produced initially with water depth of 4 mm day-1, which must be switched to 12 mm day -1after 60 days and remain so until the end of production.
Balfourodendron riedelianum da família Rutaceae é uma espécie florestal nativa com valor madeireiro, sendo utilizada na fabricação de móveis de luxo, construção civil, entre outros. Além disso, a espécie é indicada para uso medicinal, inseticida, paisagismo e recuperação de áreas ripárias. Apesar da importância desta espécie, há escassez de estudos em relação à tecnologia de sementes, à irrigação na produção de mudas e crescimento inicial a campo. Nesse sentido, foram coletados diásporos da espécie em Nova Palma (RS), em 2013 e 2015, após beneficiados e divididos em amostras para a realização dos testes. O estudo foi dividido em três capítulos, no Capítulo I - foi mensurado a biometria, tratamentos pré-germinativos e substratos para germinação de diásporos de B. riedelianum; sendo utilizado uma amostra do lote de 2013 para caracterização física, por meio do peso de mil sementes (PMS), teor de água (TU) e biometria, além do teste de germinação (G%) em diferentes substratos após superação da dormência. As dimensões dos diásporos de B. riedelianum apresentam em média, 18,59; 9,03 e 9,38 mm de comprimento; largura e espessura, respectivamente. A dormência foi superada pela imersão em água fria (8 ± 2 °C), por 48 horas e germinação pode ser conduzida em substrato vermiculita. No Capítulo II - foram realizados dois experimentos, no primeiro, foi avaliado a qualidade dos diásporos coletados em 2013 que foram armazenados durante dois anos em câmara fria e úmida, sendo retiradas amostras para testes de TU, G% e sanidade, no segundo foi avaliado no lote coletado em 2015 a desinfestação dos diásporos na incidência de fungos e na germinação. Os diásporos de B. riedelianum mantiveram-se viáveis durante 12 meses sob as condições de armazenamento, e a desinfestação com Orthocide® é indicado, tendo em vista, que proporcionou elevada G% e IVG. Os gêneros Aspergillus sp., Fusarium sp., Cladosporium sp. e Penicillium sp. foram detectados em ambos os experimentos. No Capítulo III – foi identificado a lâmina de irrigação necessária para otimizar o crescimento das mudas de B. riedelianum, e avaliado seu crescimento inicial a campo. Foram utilizados combinações entre as lâminas 4, 8 e 12 mm dia-1, que após 180 dias de aplicação do manejo hídrico realizou-se avaliações da sobrevivência (Sob), altura (H), diâmetro do coleto (DC), massa seca aérea (MSA), massa seca radicular (MSR) e índice de qualidade de Dickson (IQD). Os tratamentos descritos no experimento do viveiro foram conduzidos a campo, que aos 450 dias após o plantio foram avaliados em relação a H, DC, MSA, área foliar e comprimento radicular, teor de clorofila e fluorescência da clorofila a. As lâminas influenciaram no crescimento das mudas de B. riedelianum, na fase de viveiro, o que foi confirmado no campo. Mudas de Balfourodendron riedelianum podem ser produzidas, inicialmente, com lâmina de irrigação de 4 mm dia-1, a qual deve ser alternada para 12 mm dia-1 a partir dos 60 dias, permanecendo assim até o final da produção.

For the transformation of a seed to a seedling complex physical and biochemical changes occur within a seed before germination can proceed. Germination is controlled by diverse seed dormancy mechanisms in plant species that delays germination until the conditions are most favourable for seed germination and seedling establishment (Thompson 1970). Baskin and Baskin (1998) identified four benefits for the evolution of seed dormancy in plants: (i) persistence in risky environments as seed banks, (ii) decreased intraspecific competition, (iii) improved chances of seedling establishment and (iv) increased fitness (seed production) of the individual and the species as a whole. They showed that seed dormancy may be caused by any one of physiological, morphological, physical, chemical and mechanical constraints or by a combination of more than one of these factors. For instance, seeds may possess an embryo with a physiological inhibiting mechanism, immature embryo, impermeable seed coat or may contain chemical inhibitors and hard woody fruit walls. In all of these cases seed dormancy is eventually broken by one or more of the following treatments: after ripening, heat treatment, cold temperature stratification, prolonged exposure to high temperatures, exposure to light, softening of seed coat by microbes or physical scarification, leaching of inhibiting chemicals, ageing of seeds and other subtle changes in the habitat. In temperate North America with snow cover during winter months the seeds of a large majority of sand dune species—Cakile edentula, Ammophila breviligulata, Calamovilfa longifolia, Iva imbricata, Croton punctatus, Uniola paniculata—and others require cold stratification at <4°C for 4–6 weeks to break their dormancy requirements. Seeds of some species such as A. breviligulata and U. paniculata that require cold stratification at the northern end of their range lose this requirement in the south (Seneca 1972). At southern locations exposure to high temperatures may be required to fulfil the dormancy requirements. Winter annuals, Vulpia ciliata, Cerastium atrovirens, Mibora minima and Saxifraga tridactylites, that grow and mature their seeds in early summer on sand dunes at Aberffraw, North Wales, require exposure to high soil temperatures to overcome a state of dormancy in a certain proportion of seeds at the time of dispersal (Carey and Watkinson 1993; Pemadasa and Lovell 1975).

This chapter shows that at the end of the Cultural Revolution they did not suffer total destruction, as had the city’s walls and many popular spaces, as well as forms of folk entertainment. Teahouses were indeed dormant, but not dead; they recovered immediately once conditions were politically favorable, as seen in the renewed popularity of street-corner teahouses and emergence of the high-end tea balconies. Beginning in the late 1970s and early 1980s, teahouses re-emerged on every corner in the city and today thrive as never before.

Universities and colleges are embracing social media as a tool to spread the message about their institutions. Common uses include recruiting new students, connecting with current students, and staying connected with alumni. Nonprofit organizations in the United States also consider social media an important part of their fundraising toolbox, but use it more for recruiting volunteers, advocacy, and fundraising. Colleges and universities are also seeing the need to use social media for development purposes, whether they are private or state-supported institutions. This chapter explores how universities are using Twitter to promote year-end giving. Findings from this research suggest that while some universities seem to effectively use social media, others are inconsistent and even dormant in their messaging.

Rooted plants can often be obtained and transferred from one environment into another either in order to increase the number of vines producing a specific grape in a vineyard or to introduce a new variety or propagate a new cultivar. It has been found that some vines can be grown from hardwood cuttings. The technique of hardwood cutting involves removing a cane (Figure 4.1, a and b) from a successful vine once the vine has gone dormant for the winter, trimming it appropriately, and then planting it in well-fertilized soil either with or without growth stimulants (i.e., phytohormones, vide supra). It is clear that the conditions of planting, reported by various sources, are a function of variety and terroir. Interestingly, it appears that the cutting, which may have been grown on a rootstock different from the variety of grape produced, will produce roots that are true to the variety of grape. Once the vine, from seed, grafting, or cane begins to grow, it must be “trained” so that its growth can be monitored and successful grape crops harvested. The training includes proper spacing of vines and the establishment of a trellis system or posts for each vine. Trellis systems are set up during the first or second year of the growth of the vine since harvesting of grape crops before the third year is rare. The trellis, which will need to bear the weight of the vine and grapes, is built much like a fence. Thus, the row of grape vines is held up by end posts at the end of the row and line posts about 20 feet apart between the ends. Usually, there is a line post for every two or three vines with some species needing more space than others. Generally the end posts are thick treated wood, concrete, or steel and are strongly anchored. The line posts are thinner, and the trellis itself is made of twelve (12) gauge or heavier wire with the number of wires a function of the weight to be supported and the height to which the grapes are to be grown.

Environmental-assisted cracks in pipeline steels usually undergo the following three sequential stages prior to the failure: • Stage 1 – crack initiation and early stage crack growth, in which cracks initiate at imperfections but grow slowly depth-wise with time. Crack length may be seen to increase either because of merging with new small cracks in the vicinity of an existing crack or faster crack growth at the crack tip. Some cracks pose little threat to pipeline steel integrity if they remain dormant. • Stage 2 – Increased crack growth rate where crack growth can be dictated by mechanical driving forces and crack growth rate increases with time. • Stage 3 – The final stage of crack growth where crack growth rate is very high. Typical crack management programs mitigate cracks prior to entering Stage III. It is of great importance that pipeline steels with Stage II cracks are detected, monitored, and managed to ensure operational pipeline integrity. Although a range of crack in-line inspection and detection techniques with varied detection limits are available, it is not clear how their detection limits match the threshold geometrical dimensions of Stage 2-cracks. This investigation is aimed to define critical geometrical dimensions of cracks that are considered to be Stage 2 cracks. The determination of critical geometrical dimensions of Stage 2 cracks was made with a consideration of a wide range of situations including pipeline operating conditions, susceptible environments for crack growth, metallurgical, fabrication and construction conditions of pipeline steels. A comparison of the threshold geometrical dimensions of Stage 2 cracks with the crack detection limits of modern crack inspection and detection techniques are made at the end of the paper.