Academic literature on the topic 'Seedling production'
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Journal articles on the topic "Seedling production"
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Pangestuti, P. E. R. Prahardini, R. Rosliani, et al. "Seedling production management of Shallots from seeds based on local wisdom technology." IOP Conference Series: Earth and Environmental Science 1230, no. 1 (2023): 012213. http://dx.doi.org/10.1088/1755-1315/1230/1/012213.
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Abstract True seed shallot (TSS) has become an alternative material for shallot seeds to fulfil the lack of good-quality seed bulbs. Transplanting was the most popular TSS planting method for farmers. This technic uses seedlings as planting material. The seedling production was the most crucial stage in transferring technology from vegetative propagation (seed bulb) to generative propagation (TSS). This paper reviews the advantages and disadvantages of several seedling techniques and the strategy for selecting techniques and maintaining seedlings that are suitable to the specific conditions at the seedling site using local wisdom technology. Data and information were tracked and selected then organized in review writing paper. Analysed and data synthesis showed that several techniques could be used to produce shallot seedlings, including technical seedling, use of seedling media, seed treatment and seedling harvest arrangements. The selection of seedling techniques and maintenance management is influenced by several factors, including environmental factors of cultivation, capital availability, labour and other site-specific conditions. Seedling production can be developed as a new unit system (seedling grower) to produce excellent quality propagation material. The implications of this paper are expected to be used by seedling growers to improve the best practice of seedling production with local wisdom technology.
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Cregg*, Bert. "Defoliating Lilac Seedlings in Field Production." HortScience 39, no. 4 (2004): 820A—820. http://dx.doi.org/10.21273/hortsci.39.4.820a.
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Lilac (Syringa vulgaris L) seedlings are commonly grown in many seedling nurseries in Michigan. Typically seedlings are lifted in the fall and stored prior to shipment or stored by the customer. A major problem in field production of lilacs is that seedlings often retain their leaves late in the fall. If the leaves are not removed prior to storage or shipment, the seedlings will mold and deteriorate. Therefore, growers must spend additional labor to remove the leaves, often by hand. The goal of this research was to evaluate chemical alternatives to defoliate lilac seedlings in field nurseries. Two on-farm research trials were conducted in 2001 and 2003 in cooperation with a seedling grower in Saugatuck, MI. In Experiment 1, Florel (1/2 and ¼ dilution) and chelated copper (0.5% and 1% solution) were sprayed by and onto lilac in the seedling bed. Florel and chelated copper effectively reduced leaf area of lilac seedlings. Less than 20% of the initial leaf area remained on the 1% copper and ½ Florel-treated seedlings. The ½ Florel and 1% chelated copper completely defoliated 67% and 40% of the seedlings, respectively, whereas only 17% on the control seedlings lost all their leaves prior to lifting. Both levels of Florel and the 1% copper treatment reduced growth of seedlings after planting. In experiment 2, we applied chelated copper treatments at varying rates (0.25% and 0.5%) and times (1 application and 2 applications) using the cooperators' spray equipment. Repeated applications of chelated copper were more effective in reducing seedling leaf area than a single application at both concentrations tested.
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Jellani, G., H. Ullah, M. Ali, TN Khan, MJ Atif, and M. Musa. "Impact of seedlings age on vegetable Marrow production." SAARC Journal of Agriculture 13, no. 1 (2015): 216–22. http://dx.doi.org/10.3329/sja.v13i1.24195.
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A study was conducted at the research field of Horticultural Research Institute, National Research Center (NARC), Islamabad, Pakistan during two consecutive years of 2011 and 2012 to compare the performance of seedlings raised in polythene bags with traditional direct seeding in respect of early harvesting and profitability. Seedlings of three different ages (40, 50 and 60 days old) of vegetable marrow were compared with traditional direct seeded crop. Transplanted seedlings of vegetable marrow produced fruits 36-38 days earlier and availability period of 34-38 days longer than direct seeded crop. Similarly, higher fruit plant-1, fruit weight plant-1 and fruit yield ha-1 were obtained from seedling treatments. Averaged over years, 50-69% increase in yield and 64-100% increase in profit were recorded in seedling treatments than direct seeded crop. Among seedling ages, 60 days old seedlings were the best performer. The overall results of two years study revealed that seedling transplantation was superior to traditional direct seeded practice of growing vegetable marrow and among those 60 days old seedlings was the best in respect of yield components, fruit yield and profit.SAARC J. Agri., 13(1): 215-222 (2015)
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Garcia-Barreda, Sergi, Sara Molina-Grau, and Domenech Santiago Reyna. "Fertilisation of Quercus seedlings inoculated with Tuber melanosporum: effects on growth and mycorrhization of two host species and two inoculation methods." iForests 10, no. 1 (2016): 267–72. https://doi.org/10.3832/ifor2096-009.
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Modern truffle cultivation is based on use of inoculated seedlings, which should exhibit highly colonised roots as well as a vegetative quality enhancing field plant performance. However, poor shoot and fine root growth has been a frequent issue in inoculated <em>Quercus</em> seedlings production. Fertilisation is a common solution in forest nurseries, but high fertilisation levels have been found to inhibit the formation of ectomycorrhizas of many fungal species. The influence of slow-release fertilisation (52 mg N, 26 mg P and 36 mg K per seedling) on growth and ectomycorrhizal status of <em>Tuber melanosporum</em>-inoculated seedlings was evaluated. Host species <em>Quercus ilex</em> and <em>Quercus faginea</em> and inoculation methods involving root-dipping and root-powdering were tested. Fertilisation increased weight of both host species without significant detrimental effects on ectomycorrhizal colonisation, showing that it can be effectively used in inoculated seedlings production. Both host species showed similar response to fertilisation. The inoculation method affected seedling weight and ectomycorrhizal status, suggesting that some inoculant carriers are able to damage <em>Quercus</em> development and <em>T. melanosporum</em> colonisation. The study provided an important basis for fine-tuning the use of fertilisers in truffle-inoculated seedling production.
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Riikonen, Johanna, and Jaana Luoranen. "Seedling Production and the Field Performance of Seedlings." Forests 9, no. 12 (2018): 740. http://dx.doi.org/10.3390/f9120740.
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The rapid establishment of seedlings in forest regeneration or afforestation sites after planting is a prerequisite for successful reforestation. The relationship between the quality of the seedling material and their growth and survival after outplanting has been recognized for decades. Despite the existence of a substantial amount of information on how to produce high-quality seedlings, there is still a need to develop practices that can be used in nurseries and at planting sites to be able to produce well-growing forest stands in ever-changing environments. This Special Issue of Forests is focused on seedling quality and how it can be manipulated in a nursery as well as how the quality of the seedlings affects their field performance after planting.
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N’Danikou, S,, E. G. Achigan-Dako, D. A. Tchokponhoué, F. Assogba Komlan, R. S. Vodouhè, and A. Ahanchédé. "Improving seedling production for Vitex doniana." Seed Science and Technology 43, no. 1 (2015): 10–19. http://dx.doi.org/10.15258/sst.2015.43.1.02.
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S.H.S. Senarathne. "Effect of Arbuscular Mycorrhizal Fungi Based Biofertilizer on Coconut Seedlings Growth in Nursery." CORD 34, no. 2 (2018): 12. http://dx.doi.org/10.37833/cord.v34i2.17.
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The success of a coconut plantation, in terms of its field establishment as well as its future performance, is heavily dependent on the quality of the seedlings used. Coconut nurseries adopt different agronomic practices to produce good quality seedlings. Applying mycorrhizal inoculants is becoming an increasingly common practice in plant nurseries as it facilitates healthy seedling growth resulting in healthy and vigorous seedlings in the nursery, helps to reduce mortality rates of seedlings in the field and reduce water and fertilizer consumption. In this study effects of mycorrhizae based biofertilizer were evaluated for the growth of coconut seedlings in the nursery and field. The experiment was conducted at the Makadura Research Center in Sri Lanka. The nursery experiment treatments were, the presence of AMF based biofertilizer (50g per seedling or per bag) and absence of mycorrhizae based biofertilizer. In the field experiment, five treatments with 500g of AMF biofertilizerwith 25% recommended inorganic fertilizer mixture for young coconut palms (YPM) per seedling, 500g of AMF biofertilizer with 50% YPM per seedling, 500g of AMF biofertilizer with 75% YPM per seedling, 500g of AMF biofertilizer with 100% YPM per seedling and with only YPM per seedling. Root and shoot growth of seedlings were measured at monthly intervals in the nursery experiment. Leaf production rate and stem girth of the seedling were measured at six months interval in the field experiment. The application of bio fertilizer increased the volume and dry weight of primary, secondary, tertiary and quaternary roots in seedlings in the nursery significantly. A significantly higher leaf production rate and stem girth was observed in the field seedlings treatment with 500g of AMF biofertilizer with 75% YPM. The experiment concluded that application of bio fertilizer is beneficial for coconut seedlings in the nursery for the production good quality seedlings with well-developed roots resulting in better field establishment and in the field for fast and vigorous growth.
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Bannor, Richard Kwasi, Helena Oppong-Kyeremeh, Steffen Abele, Frank Osei Tutu, Samual Kwabena Chaa Kyire, and Dickson Agyina. "Seedling production and choice among cashew farmers in Ghana: a profitability analysis." World Journal of Entrepreneurship, Management and Sustainable Development 16, no. 2 (2020): 109–29. http://dx.doi.org/10.1108/wjemsd-11-2019-0089.
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PurposeThe unavailability and inadequate use of cashew seedlings for propagation are part of the challenges facing the cashew sub-sector in Ghana. However, promoting investment into cashew seedling production should be based on the analysis of the profitability and viability of such a venture as well as the respective determinants of farmers' demand for the planting material.Design/methodology/approachThis study used gross margin/contribution, net margin and contribution ratios to analyse the profitability of cashew seedling production under four different business models. Also, the determinants of choice of planting material for cashew plantation among farmers was analysed via a multinomial probit regression.FindingsThe study revealed that cashew seedling production is profitable with a gross margin of $8,474, $2,242, $1,616 and $1,797 and contribution to sales of 31–53% for the various business models. The positive determinants of the use of cashew seedlings were off-farm job participation and extension contact, whereas farm size and age of plantation negatively influenced the use of seedlings. Land acquisition method also influenced the use of both seedlings and seeds negatively.Practical implicationsThe findings provide empirical evidence of the viability and profitability of cashew seedling production as a viable business venture and off-farm opportunity in rural areas. The information from the study will help major stakeholders in cashew production to understand the type of farmers who use seeds and seedlings as well as the reasons for using or otherwise.Originality/valueSignificant research in the cashew value chain had focussed on the profitability of cashew plantation with little literature on profitability and viability analysis of cashew seedling production. Similarly, this study provides a significant value chain job opportunity as well as literature on the choice of cashew seedlings among current and prospective end-users.
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Brewer, H. L. "AUTOMATED SEEDLING PRODUCTION." Acta Horticulturae, no. 187 (May 1986): 53–60. http://dx.doi.org/10.17660/actahortic.1986.187.6.
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Tuzel, Y., and G. B. Oztekin. "Organic seedling production." Acta Horticulturae, no. 1170 (July 2017): 1141–48. http://dx.doi.org/10.17660/actahortic.2017.1170.147.
Full textDissertations / Theses on the topic "Seedling production"
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Pereira, Marcelo Neiva. "Lettuce seedling production (Lactuca sativa L.) in floating hydroponic system." Universidade Federal do CearÃ, 2004. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9218.
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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico<br>Although it had been early developed aiming to search on the plants mineral nutrition, the hydroponic gained more importance between the growers due to possibility a better nutritional control that confers greater plant quality and minor incidence of pests. Some hydroponic systems have been considered for the lettuce grown, between them NFT (Nutrient Film Technique) and Floating System. This last one consists of the use of great amounts of nutrient solution in tanks where the plants are floating on plates with its roots flooded. This system has been showing promising for hot climates where the high temperatures can to limit the growing in other systems as the NFT. Therefore, a trial was carried out to evaluate the floating system in its diverse characteristics as stability of the electrical conductivity and pH, availability of dissolved oxygen to the plants and temperature of the nutrient solution. Also was evaluated, through the total fresh weight and root length, the cultivars VerÃnica and Vera, which are spread out between the growers that use hydroponic systems, in regards to its adaptability to the floating system. Each one of the cultivars were submitted at two levels of electrical conductivity (1,5mS/cm and 2,5 mS/cm) and two levels of artificial aeration (20min/h and 60min/h) in split-split-plot design. The study showed that the cultivar Vera was significantly better than VerÃnica concerning to the total fresh weight and root length. It did not have significant difference between the studied levels of electrical conductivity and artificial aeration in the development of the plants. It could still be observed that the system revealed sufficiently steady in regards to the evaluated parameters.<br>Embora tenha sido desenvolvida primeiramente com o objetivo de pesquisar sobre a nutriÃÃo mineral das plantas, a hidroponia ganhou bastante importÃncia entre os produtores devido à possibilidade do controle nutricional mais estreito que confere maior qualidade Ãs plantas e menor incidÃncia de problemas fitossanitÃrios. VÃrios sistemas hidropÃnicos tÃm sido propostos para o cultivo de alface, entre eles o NFT (Nutrient Film Technique) e o sistema de leito flutuante (Floating System). Este Ãltimo consiste da utilizaÃÃo de grandes quantidades de soluÃÃo nutritiva dispostas em tanques onde as plantas ficam flutuando sobre placas de material leve com suas raÃzes imersas. Este sistema tem se mostrado promissor para climas quentes onde as altas temperaturas podem ser limitantes ao cultivo em outros sistemas como o NFT. Diante do exposto foi realizado trabalho no sentido de avaliar o sistema de leito flutuante em suas diversas caracterÃstica como estabilidade da condutividade elÃtrica e pH, disponibilidade de oxigÃnio dissolvido Ãs plantas e temperatura da soluÃÃo nutritiva. TambÃm foram avaliadas, atravÃs da matÃria fresca total e comprimento radicular, os cultivares VerÃnica e Vera, os quais sÃo os mais difundidos entre os produtores que utilizam o sistema hidropÃnico, quanto à sua adaptabilidade ao sistema de leito flutuante. Para isso cada cultivar foi submetido a dois nÃveis de condutividade elÃtrica (1,5mS/cm e 2,5 mS/cm) e dois nÃveis de aeraÃÃo artificial (20min/h e 60min/h) utilizando o delineamento em parcelas subsubdivididas. O estudo mostrou que o cultivar Vera foi significativamente superior ao VerÃnica em relaÃÃo à matÃria fresca total e comprimento radicular. NÃo houve diferenÃa significativa entre os nÃveis de condutividade elÃtrica e aeraÃÃo artificial estudados no desenvolvimento das plantas. PÃde-se observar ainda que o sistema mostrou-se bastante estÃvel em relaÃÃo Ãs variÃveis avaliadas.
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Olave, Rodrigo Javier. "Tree seedling production for multifunctional land use systems in cool temperate climates." Thesis, Queen's University Belfast, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426722.
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Oddie, Rachel Louise Annabelle. "Eucalyptus camaldulensis x globulus hybrids for saline land: Production, seedling growth and salt / waterlogging tolerance." Thesis, Oddie, Rachel Louise Annabelle (1996) Eucalyptus camaldulensis x globulus hybrids for saline land: Production, seedling growth and salt / waterlogging tolerance. PhD thesis, Murdoch University, 1996. https://researchrepository.murdoch.edu.au/id/eprint/52423/.
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The rapid development of Eucalyptus globulus Labill. plantations for the paper and pulp industry and the increasing problem of dry land salinity in the south west of Western Australia, stimulated the development of a breeding programme aimed at producing a Eucalyptus L'Herit. hybrid that combines the salt / waterlogging tolerance of selected E. camaldulensis Dehnh. clones with the high pulp quality and yield of selected E. globulus subsp. globulus families.
Controlled pollination techniques were used to produce intraspecific and interspecific hybrids. Unilateral cross incompatibility was observed between the two species. When E. globulus was used as the female parent E. camaldulensis pollen tubes failed to grow the entire length of E. globulus styles. However when E. camaldulensis was used as the female parent, interspecific crosses were successful. Five E. camaldulensis salt tolerant clones and four E. globulus families (three with known pedigree) were used to produce the crosses.
E. camaldulensis flowers were emasculated just before operculum shed. Stigma receptivity, assessed by seed production, peaked in E. camaldulensis three days after emasculation. This coincided with the style turning red and the stigma becoming enlarged, yellow and sticky. Reasonably high levels of seed (> 25 seeds per capsule and > 65 % capsule set) could also be produced when flowers were pollinated before or after day three (days 0 - 5). Pollen placed on green, dry stigmas (days 0-2) remained ungerminated until stigmas began to enlarge and become sticky. The timing of stigma receptivity varied between the E. camaldulensis genotypes.
The number of seeds produced per capsule when E. camaldulensis was selfed was 87 % less than when the clones were outcrossed. The interspecific hybrids showed a 77 % reduction in seed set compared to outcrossed E. camaldulensis. In both cases the mechanism of the partial incompatibility was not operating in the style.
Further barriers to the hybridization of E. camaldulensis and E. globulus were manifested as a high percentage of seedlings with abnormalities compared to outcrossed E. camaldulensis. The intensity of barriers to interspecific hybridization varied, with families exhibiting from 50 to 97 % abnormal progeny.
Seedlings at the two leaf stage and at six to eight months old were scored for a number of morphological characters. These were cotyledon length, cotyledon width, depth of cotyledon emargination, petiole length, lamina length, lamina width at the widest point, the vertical distance between the axillary buds at the fifth node and leaf angle (angle between the main stem and the midvein of the lamina). Overall the morphology of the hybrids (assessed by these characters) tended to be intermediate between the two parents, but more like E. globulus than E. camaldulensis.
A small number of seedlings produced from E. camaldulensis x globulus crosses were morphologically very similar to E. camaldulensis. Allozyme analysis was used to determine the parentage of these individuals. Of the six enzyme systems studied malate dehydrogenase (MDH, E.C. 1.1.1.37) and 6-phosphogluconate dehydrogenase (6PGD, E.C. 1.1.1.44) were the most useful in identifying E. camaldulensis x globulus hybrids. The seedlings from hybrid families with similar morphology to E. camaldulensis were identified as E. camaldulensis. All other seedlings from hybrid families were clearly of hybrid origin. The possible cause of the seed contamination in the controlled crosses is discussed.
Seven month old seedlings were screened for salt / waterlogging tolerance in a glasshouse trial. Seedlings were placed in tanks and flooded to the sand level in the pots with one fifth Hoagland nutrient solution number two for one week. The following and each subsequent week 50 mM NaCl was added to the tanks until the NaCl concentration reached 350 mM. This concentration was held for three weeks. E. camaldulensis had a greater salt / waterlogging tolerance than E. globulus based on survival and height growth rate. The performance of the hybrid families was intermediate between the two parental species. Genotypes with superior tolerance of saline waterlogging were micropropagated for further field trials.
The potential of selected E. camaldulensis x globulus genotypes is discussed in light of the preliminary results of the breeding programme.
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Cummings, Jaime Anne. "Evaluation of seed and drench treatments for management of damping-off and seedling blight pathogens of spinach for organic production." Online access for everyone, 2007. http://www.dissertations.wsu.edu/Thesis/Fall2007/j_cummings_112007.pdf.
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Bagatim, Amanda Garcia [UNESP]. "Temperatura e substrato na germinação de Physalis angulata L." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/150341.
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Previous issue date: 2016-11-04<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Devido as escassas informações sobre as melhores condições para a produção de mudas de physalis no Brasil, este trabalho teve o objetivo de verificar a temperatura adequada para germinação, assim como o substrato mais eficiente para a emergência de plântulas de Physalis angulata L. As sementes após serem extraídas de frutos maduros, foram lavadas para a retirada de toda mucilagem e secas em temperatura ambiente (±25 ºC) por 24 horas. Após este período, estas foram colocadas em câmaras de germinação tipo B.O.D., com temperaturas controladas e submetidas a diferentes temperaturas: 20, 25, 30, 35 e 20-30 °C, para posterior avaliação da porcentagem de germinação, índice de velocidade de germinação e tempo médio de germinação. Um segundo experimento foi realizado testando diferentes substratos, onde foram utilizados: vermiculita textura fina, mistura de terra + areia + esterco de curral curtido (3:1:1), substrato comercial à base de casca de pinus e fibra de coco. As sementes após passarem pelo processo de limpeza e secagem descrito acima, foram semeadas em bandejas de polietileno e avaliadas quanto à porcentagem de emergência, índice de velocidade de emergência e tempo médio de emergência. Foram coletadas amostras aleatórias das plântulas e estas levadas para laboratório, onde foram avaliados: comprimento médio de parte aérea e raiz (cm), massas fresca e seca de raiz e parte aérea (gramas) e número de folhas. Para ambos os experimentos o delineamento foi o inteiramente casualizado (DIC), os resultados submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. De acordo com os resultados obtidos, não há influência dos substratos testados na germinação de sementes de Physalis angulata L., sendo o substrato comercial à base de casca de pinus o que proporcionou melhor desenvolvimento inicial das mudas. Já as melhores temperaturas para a germinação de sementes de physalis foram: alternada 20-30 ºC e constantes 30 e 35 ºC, com maiores índices de velocidade de germinação para a temperatura alternada 20-30 ºC.<br>Due to the scarce information about the best conditions for the production of physalis seedlings in Brazil, this research had the objective to verify the adequate temperature for germination, as well as the most efficient substrate for the emergence of Physalis angulata L. seedlings. After being extracted from ripe fruits, seeds were washed to removal all the mucilage and dried at temperature ± 25 °C for 24 hours. After this period, the seeds were placed to germinate in chambers type BOD, with controlled temperatures: 20, 25, 30, 35 and 20-30 °C, for further evaluation of germination percentage, germination speed index and average germination time. A second experiment was carried out aiming to test different substrates: vermiculite fine texture, soil + sand mixture + tanned corral manure (3:1:1), commercial substrate with pine bark and coconut fiber. The seeds, after the cleaning and drying process described above, were seeded in polyethylene trays and evaluated for the percentage of emergence, rate of emergence and mean time of emergence. Random samples were collected from the seedlings and taken to the laboratory, evaluating: average length of shoot and root (cm), fresh and dry masses of root and shoot (grams) and number of leaves. For both experiments the design was completely randomized (DIC), the results submitted to the analysis of variance and the means compared by the Tukey test at 5% of probability. According to the results obtained, there is no influence of the substrates tested on the germination of seeds of Physalis angulata L., being the commercial substrate with pinus bark which provided better initial development of the seedlings. The best temperatures for the germination of physalis seeds were: alternated 20-30 ºC and constants 30 and 35 ºC, with higher rates of germination for the alternating temperature of 20-30 ºC.<br>CNPq: 163880/2014-0
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Timm, Cari Rejane Fiss. "Enraizamento, dinâmica e protocolo de propagação de Prunus." Universidade Federal de Pelotas, 2016. http://repositorio.ufpel.edu.br:8080/handle/prefix/3116.
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Previous issue date: 2016-08-31<br>Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq<br>Existem muitas informações referentes à cultura do pessegueiro. Entretanto, como o
dinamismo da pesquisa, da fruticultura e do mercado consumidor causa mudanças,
estas irão exigir um sistema moderno e tecnificado de produção de mudas de
qualidade genética e sanitária para obtenção de frutos de qualidade e uma alta
produtividade. Com o objetivo de adequar uma metodologia de propagação para a
produção de mudas de qualidade, foram desenvolvidos vários experimentos com
cultivares copa e porta-enxertos de Prunus spp. para avaliar os efeitos de diferentes
concentrações de ácido indolbutírico e do tipo de miniestaca no enraizamento,
determinar o tempo ótimo de enraizamento de miniestacas e identificar nas fases de
estabelecimento e multiplicação o meio de cultura e a concentração de BAP
adequados, na propagação in vitro para obtenção de mudas. Os experimentos
foram realizados em casa de vegetação e no Laboratório de Micropropagação de
Plantas Frutíferas do Departamento de Fitotecnia da Faculdade de Agronomia Eliseu
Maciel da Universidade Federal de Pelotas, no período de março de 2014 a fevereiro
de 2016. Para os experimentos de enraizamento foram utilizados os porta-enxertos
das cultivares Nemaguard, Flordaguard, Okinawa, umezeiro, Mr.S 2/5 e as cultivares
copa Capdeboscq, Bonão e Maciel, testando-se os tipos de miniestacas, as
diferentes concentrações de AIB e determinando o tempo ótimo de enraizamento
das miniestacas. No estabelecimento e na multiplicação foram utilizados os portaenxertos umezeiro e Marianna para determinar o meio de cultura e a concentração
de BAP. No enraizamento, as miniestacas apicais, medianas e basais resultaram
com a maior porcentagem utilizando-se 1.000 mg L-1 de AIB e determinando o tempo
ótimo de enraizamento Flordaguard obteve 70% aos 50 dias de cultivo. Na fase de
estabelecimento observou-se que o meio de cultivo WPM acrescido de 1.0 mg L-1 de
BAP demonstrou ser o mais adequado. Na multiplicação, verificou-se que a cultivar
Marianna com 1.5 mg L-1 de BAP foram superiores para altura da brotação, número
de gemas, número e comprimento das brotações massa fresca e taxa de
multiplicação.<br>There are many information regarding the peach crop. However, as the dynamism of
research, horticulture and consumer market causes changes, these will require a
modern and technified production of genetic and health quality system to obtain
seedlings of fruit quality and high productivity. In order to adapt a method of
propagation for the production of quality seedlings, they were developed several
experiments with cultivars crown and rootstock Prunus spp. to evaluate the effects of
different indolebutyric acid concentration and type of minicuttings rooting,
determining the optimum time of cuttings rooting and identify the establishment and
multiplication stage culture medium and concentration of appropriate BAP in vitro
propagation in obtaining seedlings. The experiments were conducted in a
greenhouse and Fruit trees Plant Micropropagation Laboratory of the Department of
Plant Science, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas,
from March 2014 to February 2016. For rooting experiments were used rootstocks of
Nemaguard cultivars Flordaguard, Okinawa, mume, Mr.S 2/5 and cultivars
Capdeboscq, Bonao and Maciel, testing themselves the types of cuttings, different
concentrations of IBA and determining the optimum time for rooting of cuttings. In the
establishment and multiplication were used the rootstocks mume and Marianna to
determine the culture medium and the concentration of BAP. In the rooting cuttings
apical, middle and basal resulted with the highest percentage using 1,000 mg L-1 IBA
and determining the rooting great time Flordaguard obtained 70% after 50 days of
cultivation. In the establishment phase it was observed that the WPM culture medium
supplemented with 1.0 mg L-1 BAP proved to be the most suitable. In multiplying it
was found that cultivating Marianna 1.5 mg L-1 BAP were superior to the height of the
time of budding e, number and length of shoot fresh weight and multiplication rate.
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Shiroma, Amarilís Thieme. "Sistema de indicadores aplicado à agricultura irrigada." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11152/tde-15032019-125427/.
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O manejo da irrigação na produção de mudas de hortaliças é caracterizado como uma atividade complexa, pois são cultivadas, em um mesmo ambiente, diversas espécies com necessidades hídricas e estádios fenológicos diferentes. Além disso, este setor está inserido em um mercado extremamente competitivo, o que faz com que a melhoria contínua dos processos se torne essencial para a sobrevivência do negócio. Em função da importância do sistema de medição de desempenho como instrumento de auxílio à gestão, o presente trabalho teve como objetivo propor um conjunto de indicadores de desempenho que permita a avaliação dos processos operacionais de produção de mudas de hortaliças folhosas. O estudo, classificado como uma pesquisa qualitativa do tipo exploratória, foi desenvolvido a partir do referencial teórico e de informações técnicas obtidas nos viveiros. O trabalho foi dividido, basicamente, em três etapas: na definição dos objetivos da produção de mudas, no mapeamento dos processos envolvidos e, por último, na seleção dos indicadores de desempenho que mais se adequam aos processos. Foram identificados quarenta e cinco indicadores de desempenho na literatura, sendo que seis foram selecionados como indicadores-chave.<br>Irrigation management in the production of vegetable seedling is characterized as a complex activity, since several species with different water needs and different phenological stages are cultivated in the same local. In addition, this sector is embedded in an extremely competitive market, which means that the continuous improvement of processes becomes essential for the survival of the business. Due to the importance of the performance measurement system as an aid to management, the present work had the objective of proposing a set of performance indicators that allow the evaluation of the operational processes of vegetable\'s seedlings production. The study, classified as a qualitative research of the exploratory type, was developed based on the theoretical reference and the technical information obtained from the nurseries. The work was basically divided in three stages: the definition of the objectives of the production of seedlings, the mapping of the involved processes and, finally, the selection of the performance indicators that best suit the processes. Forty five performance indicators were identified in the literature, of which six were selected as key indicators.
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Silva, Tatiana Maria da. "Seed biometrics, morphology seedlings and species of five initial growth fruit that occur in Northeastern Brazil." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14130.
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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior<br>Brazilian native fruits are among the most tasty and nutritious in the world, being known only by the local population. The knowledge of the morphology of seeds and seedlings is useful in understanding the development of native species, being fundamental in the study of germination in species identification in the juvenile stage, the interpretation of tests of germination and differentiation of congeners. The study of the initial growth of plants is important for establishing standards of seedlings produced in nurseries. The objective of this work was to obtain information on biometrics seed, seedling morphology and early growth of American ximenia L, Genipa americana L., Ziziphus joazeiro Mart., Talisia esculenta Raldk and Crataeva tapia L. The experiments were conducted at the Center for Teaching and Research in Urban Agriculture (NEPAU), Department of Plant Science in CCA / UFC, Fortaleza-CE. Biometrics seeds was performed on a sample of 100 diaspores of each species. To study the morphology of seedlings made up planting 100 seeds in tubes of 288cm  s filled with vermiculite and humus in the volume ratio 2:1, except Juazeiro whose endocarps previously scarified, were sown in beds tire containing 2 offish 1 humus (v/v). We carried out daily photographic records of germination and seedling establishment process. The initial growth analysis was performed in two environments (full sun and greenhouse) during seven periods in a split plot design with six replications, in a randomized design, except for jujube plum mad and whose evaluations were performed only at home vegetation. The length values observed in âameixa-bravaâ, âgenipapoâ, âjuazeiroâ, âpitombaâ and âtrapiÃâ were 14.98; 9.64; 8.74; 18.25 and 7.72 mm respectively. âAmeixa-bravaâ and âpitombaâ presented hypogeal cryptocotyledonary germination, while the genipap âjuazeiroâ and âtrapiÃâ and were characterized as phanerocotylar epigeal. Trapià plants kept in the greenhouse showed better development. Seedlings âgenipapoâ and âpitombaâ full sunlight exhibited greater development. The pattern of seedling plum mad at 109 days: 12.13 cm, 19.02 cm root length, 3.52 mm diameters and 7.1 leaves. The âjuazeiroâ seedlings with 138 days exhibited the following pattern: height - 41.33 cm; root length - 37.84; stem diameter - 4.13 mm and number of leaves - 43.05.<br>As frutas nativas brasileiras estÃo entre as mais saborosas e nutritivas do mundo, sendo conhecidas apenas pela populaÃÃo local. O conhecimento da morfologia de sementes e plÃntulas à Ãtil na compreensÃo do desenvolvimento das espÃcies nativas, sendo fundamentais no estudo da germinaÃÃo, na identificaÃÃo de espÃcies na fase juvenil, na interpretaÃÃo dos testes de germinaÃÃo e diferenciaÃÃo de espÃcies congÃneres. O estudo do crescimento inicial das plantas à importante para o estabelecimento de padrÃes de qualidade de mudas produzidas nos viveiros. Objetivou-se neste trabalho obter informaÃÃes sobre biometria de sementes, morfologia de plÃntulas e crescimento inicial de ameixa-brava (Ximenia americana L.), jenipapo (Genipa americana L.), juazeiro (Ziziphus joazeiro Mart.), pitomba (Talisia esculenta Raldk) e trapià (Crateva trapia L.). Os experimentos foram desenvolvidos no NÃcleo de Ensino e Pesquisa em Agricultura Urbana (NEPAU), do Departamento em Fitotecnia CCA/UFC, em Fortaleza-CE. A biometria das sementes foi realizada em uma amostra de 100 diÃsporos de cada espÃcie. Para o estudo dos aspectos morfolÃgicos das plÃntulas efetuou-se o plantio de 100 sementes em tubetes de 288cm preenchidos com s vermiculita e hÃmus na proporÃÃo volumÃtrica 2:1, exceto para juazeiro cujos endocarpos, previamente escarificados, foram semeados em canteiros de pneu contendo 2 arisco : 1 hÃmus (v/v). Efetuaram-se registros fotogrÃficos diÃrios do processo de germinaÃÃo e estabelecimento da plÃntula. A anÃlise de crescimento inicial foi realizada em dois ambientes (sol pleno e casa de vegetaÃÃo) durante sete perÃodos num esquema de parcelas subdivididas com seis repetiÃÃes, seguindo um delineamento inteiramente casualizado, exceto para ameixa-brava e juazeiro cujas avaliaÃÃes foram realizadas apenas em casa de vegetaÃÃo. Os valores do comprimento observados em ameixa-brava, jenipapo, juazeiro, pitomba e trapià foram 14,98; 9,64; 8,74; 18,25 e 7,72mm respectivamente. Ameixa-brava e pitomba apresentaram germinaÃÃo hipÃgea criptocotiledonar, enquanto o jenipapo, juazeiro e trapià caracterizaram-se como epÃgea fanerocotiledonar. As plantas de trapià mantidas em casa de vegetaÃÃo apresentaram melhor desenvolvimento. As mudas de jenipapo e pitomba desenvolveram-se mais sob sol pleno. As mudas de ameixa-brava com 109 dias de idade apresentaram 12,13cm de altura, 19,02cm de comprimento de raiz, 3,52mm de diÃmetro do colo e 7,1 folhas. As mudas de juazeiro com 138 dias exibiram o seguinte padrÃo: altura â 41,33cm; comprimento da raiz â 37,84; diÃmetro do colo â 4,13mm e numero de folhas â 43,05.
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Goswami, Shinjini. "Investigating nutrient co-limitation in northern hardwood forests." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1501414320563565.
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Góes, Glêidson Bezerra de. "Propagação do tamarindeiro (Tamarindus indica L.) e da pitombeira (Talisia esculenta Raldk) por enxertia." Universidade Federal Rural do Semi-Árido, 2011. http://bdtd.ufersa.edu.br:80/tede/handle/tede/97.
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Previous issue date: 2011-02-04<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Due to the broad market prospects for crops in tamarind and pitombeira, it becomes essential the evaluation of methods that permit the propagation on a commercial scale. Considering this, two experiments were organized in a greenhouse, located in the Universidade Federal Rural do Semiárido UFERSA, located in the town of Mossoró, RN, from January 2009 until June 2010, with the objective of determining the best grafting method for the production of seedlings of tamarind and pitombeira. We studied the types of grafting: grafting in top cleft, cleft grafting in the top simple English way , grafting in the top complicated English way , grafting budding side and plate cleft. The design used in the experiments was a randomized complete block with five treatments (grafting types). The experiment with tamarind had seven repetitions; each parcel consisted of 14 seedlings, a total of 98 per treatment, adding 490 in the experiment. Variables were evaluated: percentage of grafting success, shoot length, root length, branch length, number of leaves, number of branches, stem diameter, shoot dry matter, root dry matter, Total matter dry. The data were subjected to analysis of variance and compared through the Tukey test at 5% significance using the software SISVAR. The rates of graft takes were submitted to angular transformation of arcsen √ x/100, and data on the number of branches in √ x + 0.5. In order to promote the statistic, treatment of pimple on board was disregarded, considering that this treatment has zero index of plant take. The grafting methods in top cleft, cleft grafting in the English top grafting complicated and simple English way in the top, are respectively the best methods of grafting for the tamarind. The test was conducted with pitombeira with four replications and each plot was composed of twenty seedlings in a total of 400 seedlings on the experiment, in which was observed zero rate of grafting take<br>Devido às amplas perspectivas de mercado para as culturas do tamarindeiro e pitombeira, torna-se indispensável a avaliação de métodos que permitam a propagação em escala comercial. Diante disso, foram desenvolvidos dois experimentos em casa de vegetação, localizada no Campus da Universidade Federal Rural do Semiárido UFERSA, situada na cidade de Mossoró-RN, no período de janeiro de 2009 a junho de 2010, com o objetivo de determinar o melhor método de enxertia para a produção de mudas de tamarindeiro e de pitombeira. Foram estudados os tipos de enxertia: garfagem no topo em fenda cheia, garfagem no topo à inglesa simples, garfagem no topo à inglesa complicada, garfagem em fenda lateral e borbulhia em placa. O delineamento utilizado nos experimentos foi o de blocos casualizados, com cinco tratamentos (tipos de enxertia). O experimento com tamarindeiro foi constituído por sete repetições, sendo cada parcela composta por 14 mudas, num total de 98 por tratamento, perfazendo 490 no experimento. Foram avaliadas as variáveis: percentagem de pegamento dos enxertos, comprimento da parte aérea, comprimento do sistema radicular, comprimento de ramos, número de folhas, número de ramos, diâmetro do colo, matéria seca da parte aérea, matéria seca do sistema radicular, matéria seca total. Os dados obtidos foram submetidos à analise de variância e comparados através do teste de Tukey ao nível de 5% de significância utilizando o software SISVAR. Os índices de pegamento dos enxertos foram submetidos à transformação angular do arcsen √x/100, e os dados de número de ramos em √x + 0,5. Para fins da realização da estatística, o tratamento de borbulha em placa foi desconsiderado, tendo em vista que este tratamento apresentou índice zero de pegamento. Os métodos garfagem no topo em fenda cheia, garfagem no topo à inglesa complicada e garfagem no topo à inglesa simples são, respectivamente, os melhores métodos de enxertia para o tamarindeiro. O ensaio com pitombeira foi realizado com quatro repetições sendo cada parcela composta por vinte mudas, num total de 400 mudas no experimento, no qual foi verificado índice zero de pegamento dos enxertos
Books on the topic "Seedling production"
1
Doolan, David W. Vegetable seedling production manual. Food and Agriculture Organization of the United Nations, 1999.
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2
Canada. Canadian Forestry Service. Great Lakes Forest Research Centre. Containerized seedling production statistics for Ontario, 1982. Canadian Forestry Service., 1985.
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3
McCarthy, Ellen L. Exponential fertilization regimes for containerized black spruce seedling production. National Library of Canada = Bibliothèque nationale du Canada, 1991.
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Alexander, Robert R. Engelmann spruce seed production and dispersal and seedling establishment in the central Rocky Mountains. U.S. Dept. of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station, 1986.
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5
Bickelhaupt, Donald H. Effect of horse manure on white pine seedling production in a forest tree nursery. State University of New York, College of Environmental Science and Forestry, 1987.
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Alexander, Robert R. Engelmann spruce seed production and dispersal, and seedling establishment in the central Rocky Mountains. U.S. Dept. of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station, 1986.
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James, Robert L. Diseases associated with whitebark pine seedling production, USDA Forest Service Nursery, Coeur d'Alene, Idaho. U.S. Dept. of Agriculture, Forest Service, Northern Region, 2000.
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Cook, Michael C. F. An economic review of N. Ireland apple production and marketing with special reference to Bramley Seedling. Economics and Statistics Division, 1988.
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Production, of Quality 'Bramley's Seedling' Fruit Conference (2007 Armagh Ireland). The Production of Quality 'Bramley's Seedling' Fruit Conference: Wednesday 31 January 2007 at 6:30 p.m., Armagh City Hotel, Armagh. Dept. of Agriculture and Rural Development, 2007.
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James, Robert L. An evaluation of the effects of dazomet on soil-borne diseases and conifer seedling production: USDA Forest Service Lucky Peak Nursery, Boise, Idaho. U.S. Dept. of Agriculture, Forest Service, Northern Region, 1999.
Find full textBook chapters on the topic "Seedling production"
1
Morgan, Lynette. "Propagation and transplant production." In Hydroponics and protected cultivation: a practical guide. CABI, 2021. http://dx.doi.org/10.1079/9781789244830.0008.
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Abstract Crop establishment is a fundamental process in greenhouse and hydroponic production. Seed and seedling delivery systems vary considerably between different types of crops and growing situations and with level of mechanization available. This chapter focuses on propagation and transplant production. Crop establishment methods such as propagation from seed, seedling transplant production through containerized and non-containerized delivery methods, seeding of cell flats, trays and other transplant media, transplant production systems, use of plant factories for seedling transplant production, organic transplant production, grafting, vegetative propagation, and tissue culture are discussed in this chapter.
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Morgan, Lynette. "Propagation and transplant production." In Hydroponics and protected cultivation: a practical guide. CABI, 2021. http://dx.doi.org/10.1079/9781789244830.0118.
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Abstract Crop establishment is a fundamental process in greenhouse and hydroponic production. Seed and seedling delivery systems vary considerably between different types of crops and growing situations and with level of mechanization available. This chapter focuses on propagation and transplant production. Crop establishment methods such as propagation from seed, seedling transplant production through containerized and non-containerized delivery methods, seeding of cell flats, trays and other transplant media, transplant production systems, use of plant factories for seedling transplant production, organic transplant production, grafting, vegetative propagation, and tissue culture are discussed in this chapter.
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Rehman, Hafeez ur, Muhammad Farooq, Mubashir Hussain, and Shahzad M. A. Basra. "Rice Seed and Seedling Priming." In Modern Techniques of Rice Crop Production. Springer Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-4955-4_4.
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Biddle, Julianne, Quang Nguyen, Zhihua H. Mu, Mike Foale, and Steve Adkins. "Germplasm Reestablishment and Seedling Production: Embryo Culture." In Coconut Biotechnology: Towards the Sustainability of the ‘Tree of Life’. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-44988-9_10.
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Olatunbosun Adeyemi, Nurudeen, Oni Olanrewaju Emmanuel, and Debasis Mitra. "AM Fungi Interactions in Rice Seedling Production." In Arbuscular Mycorrhizal Fungi. CRC Press, 2023. http://dx.doi.org/10.1201/9781003354086-17.
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Iakovoglou, Valasia. "Effect of Irrigation Frequency on Tree Seedling Production." In Sustainable Bioresource Management. Apple Academic Press, 2020. http://dx.doi.org/10.1201/9780429284229-15.
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Abu Hassan, Affrida, Norazlina Noordin, Zaiton Ahmad, Mustapha Akil, Faiz Ahmad, and Rusli Ibrahim. "Protocol for Mass Propagation of Plants Using a Low-Cost Bioreactor." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana. Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_11.
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AbstractConventional in vitro mass propagation methods are labour-intensive, costly and have a low degree of automation. Bioreactor or automated growth vessel systems using liquid media were developed to overcome these problems. The use of liquid instead of solid culture medium for plant micropropagation offers better access to medium components and scalability through automation. However, the cost of setting up a bioreactor system is one of its disadvantages as such systems are expensive with limited number of manufacturers. A low-cost bioreactor system was set up using recycled, low biodegradable plastic bottles. This low-cost bioreactor, based on temporary immersion principle, has proven to be effective as a vessel for rapid plant propagation. It is designed to reduce the production cost of plant micropropagation. This chapter explains the step-by-step methods for setting up a low-cost bioreactor for banana seedling production. This low-cost bioreactor system has the potential to be adapted for large scale in vitro cultivation of the plant seedlings.
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Liu, Jiaying, and Zhao Zhang. "Development and Test of an Auto Seedling Detection System." In Sensing Technologies for Field and In-House Crop Production. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-7927-1_8.
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Li, Yunxia, Zuochen Jiang, Zhao Zhang, Han Li, and Man Zhang. "SeedingsNet: Field Wheat Seedling Density Detection Based on Deep Learning." In Sensing Technologies for Field and In-House Crop Production. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-7927-1_5.
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Yamauchi, Minoru. "Rice seedling establishment as affected by soil type and redox potential." In Plant Nutrition for Sustainable Food Production and Environment. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0047-9_258.
Full textConference papers on the topic "Seedling production"
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Dong, Wenhao, Jing He, Chuandong Ji, Xiaoman Cao, and Xiaoya Zhang. "Study on Seeding Weight Detection Device of Rice Seedling Production Line." In 2024 IEEE 7th Information Technology, Networking, Electronic and Automation Control Conference (ITNEC). IEEE, 2024. http://dx.doi.org/10.1109/itnec60942.2024.10732919.
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Cacini, Sonia, Giulia Arati, Silvia Traversari, et al. "Optical Sensors for Optimizing Nitrogen Application in Broccoli Seedlings Production." In 2024 IEEE International Workshop on Metrology for Agriculture and Forestry (MetroAgriFor). IEEE, 2024. https://doi.org/10.1109/metroagrifor63043.2024.10948826.
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Nuntanart, Kunjira, Voravit Jaituy, and Papis Wongchaisuwat. "Factors Affecting Oil Palm Cultivation Using Machine Learning and Statistical Inference Methods." In 14th International Seminar on Industrial Engineering and Management. Trans Tech Publications Ltd, 2025. https://doi.org/10.4028/p-bxilf6.
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Oil palm has become the world’s leading vegetable oil with a tremendous increase in plantations and production. Thailand is among the three largest producers of oil palm. To enhance the oil palm producing potential competitively, the oil palm industry in Thailand has to improve the efficiency of production management among Thai farmers. This work aimed to identify important factors affecting oil palm cultivation based on machine learning and statistical inference methods. The proposed models were evaluated on a data set collected from the local community group for oil palm cultivation and production in Surat Thani and Nakhon Si Thammarat provinces, Thailand. The seedlings’ source and the age of oil palm seedlings were the most significant features according to the analysis.
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Balliu, Astrit, Nina Kacjan Maršić, and Nazim Gruda. "Seedling production." In VII South-Eastern Europe Syposium on Vegetables & Potatoes. University of Maribor Press, 2017. http://dx.doi.org/10.18690/978-961-286-045-5.34.
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Dumacheva, Elena, Sergey Kostenko, Vladimir Chernyavskih, Yuliya PEChEGINA, and Artem Grebennikov. "COMPARATIVE ANALYSIS OF THE RATE OF CO2 ASSIMILATION IN ANNUAL TRIFOLIUM SPECIES AT THE JUVENILE STAGE OF ONTOGENESIS." In Multifunctional adaptive fodder production. Federal Williams Research Center of Forage Production and Agroecology, 2024. http://dx.doi.org/10.33814/mak-2023-32-80-14-21.
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The parameters of photosynthesis, dark respiration and intercellular CO2 concentration were studied in three species of annual clovers: Trifolium resupinatum, T. alexandrinum, T. incarnatum. Significant differences in the parameters of the rate of carbon dioxide assimilation between the studied species were established in the experiment. The maximum value of dark respiration and CO2 assimilation rate was observed in the seedling leaves of T. alexandrinum seedlings. The equations of light curves of CO2 assimilation and intercellular CO2 concentration were calculated. High approximation coefficients were found: from 0.945 to 0.989 for photosynthesis light curves; from 0.683 to 0.983 for intercellular CO2 concentrations.
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Dumacheva, Elena, Elena USOL'CEVA, Polina MAKSIMOVA, et al. "INTRODUCTION ISSUES: INFLUENCE OF SALINITY ON GAS EXCHANGE PROCESSES OF SEEDLINGS HEDYSARUM GRANDIFLORUM PALL." In Multifunctional adaptive fodder production. Federal Williams Research Center of Forage Production and Agroecology, 2024. http://dx.doi.org/10.33814/mak-2023-32-80-104-110.
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The large-flowered sweetvetch (Hedysarum grandiflorum Pall.) is a perennial beautiful-flowering plant with a narrow ecological amplitude, promising for introduction and cultivation. Under controlled conditions using the LI-6800 instrument, LI-COR, USA, the dynamics of indicators of photosynthesis intensity, transpiration and accompanying physiological processes related to gas exchange in H. grandiflorum seedling leaves under different light levels (1500, 1000, 500, 200, 100, 50, 0 µM/m2/s) in saline conditions was studied. It has been established that photosynthetic apparatus of H. grandiflorum seedlings adapts well enough to saline conditions and significant photosynthesis suppression by 17.4 % is observed only at PAR level of 300 µM/m²/s¹. At the same time, a significant decrease in the rate of water evaporation by the surface of cotyledon leaves under the influence of salinity conditions — by 36.5–54.3 % is observed. The obtained data indicate a potentially high adaptive capacity of H. grandiflorum seedlings due to self-regulation of gas exchange processes.
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Tüzel, Yüksel, Golgen Oztekin, Ismail Hakki Tuzel, and Hale Duyar. "Growing media in organic seedling production." In VII South-Eastern Europe Syposium on Vegetables & Potatoes. University of Maribor Press, 2017. http://dx.doi.org/10.18690/978-961-286-045-5.17.
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Zolotarev, Vladimir, Iosif Ivanov, and Marina Ostrikova. "Study and evaluation of breeding material awnless brome (Bromopsis inermis Leyss.) of lawn-pasture type in steppe conditions Central Chernozem region." In Multifunctional adaptive feed production 27 (75). Federal Williams Research Center of Forage Production and Agroecology, 2022. http://dx.doi.org/10.33814/mak-2022-27-75-83-89.
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Among the perennial bluegrass grasses, the awnless brome is the most popular and widespread crop in the herbage of Russia. All varieties of awnless brome egistered in the State Register are of the hay-pasture type of use. A new source material of a awnless brome lawn-pasture eco-type seedling with high decorative properties has been created.
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"Current situation of grafted vegetable seedling industry and its mechanization development in Taiwan." In Grafting to improve fruit-vegetable production. Food and Fertilizer Technology Center for the Asian and Pacific Region, 2016. http://dx.doi.org/10.56669/dpxs1469.
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"Cultural management of citrus orchards with PF-citrus seedling." In Epidemiology and disease management of citrus HLB disease for sustainable citrus production in ASPAC. Food and Fertilizer Technology Center for the Asian and Pacific Region, 2012. https://doi.org/10.56669/nyej7338.
Full textReports on the topic "Seedling production"
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Israel, Alvaro, and John Merrill. Production of Seed Stocks for Sustainable Tank Cultivation of the Red Edible Seaweed Porphyra. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7696527.bard.
Full textAbstract:
Porphyra species (commonly known as ‘nori’ or ‘purple laver’) are edible red seaweeds rich in proteins, vitamins and other highly valued biogenic compounds. For years Porphyra has been cultured using seeded nets extended in the open sea, and its biomass consumed primarily in the Far East. While demands for international markets have increased steadily at an average of 20% per year, supplies are on the verge and not expected to meet future demands. Alternatively, land-based cultivation of seaweed has become attractive in the mariculture industry since (1) important growth parameters can be controlled, (2) is environmentally friendly and (3) perfectly matches with integrated aquaculture leading to sustainable, high quality products. During the last few years a tank cultivation technology for Porphyra has been developed at the Israeli institution. This technology is based on indoor production of asexual spores and their subsequent growth to 1-2 mm seedlings. The seedlings are then transferred to outdoor tanks and ponds when seawater temperatures drop to 20 °C, or below, and days become shorter during winter time. However, the current technology efficiently serves only about 100 m2 of ponds during one growth season. In order to produce seedlings in sufficient amounts, it is critical to address both technical and biological aspects of seedling production, securing optimal up-scale to commercial-size cultivation farms. We hypothesize that massive production of spores is related to thalli origin, thalli age and sporulation triggers, and that seedling survival and their subsequent growth potential is determined by the seawater quality and overall indoor growth conditions imposed. A series of bio-reactors were constructed and tested in which spore release and spore growth were separately studied. The main assessment criteria for optimal viability of the seedlings will be by determining their electron transport rate using PAM fluorometry and by subsequent growth and biomass yields in outdoor ponds. Altogether the project showed (1), controlled sporulation is possible in big outdoor/growth chamber settings provided initial stock material (small frozen seedlings) is at hand, (2), contamination problems can be almost completely avoided if stock material is properly handled (clean as possible and partially dehydrated prior to freezing), (3), spore release can significantly be enhance using high nutrient levels during thawing for P. yezoensis and P. haitanensis, but not for P. rosengurttii, (4), PAM fluorometry is an efficient tool to estimate growth capacity in both seedlings and juvenile thalli. The BARD funding also served to explore other aspects of Porphyra biology and cultivation. For example, the taxonomical status of Porphyra strains used in this study was defined (see appendix), and the potential use of this seaweed in bioremediation was well substantiated. In addition, BARD funding supported a number of opportunities and activities in the Israeli lab, direct or indirectly related to the initial objectives of the project such as: additional molecular work in other seaweeds, description of at least 2 new species for the Israeli Mediterranean, and continuous support for the writing of a book on Global Change and applied aspects of seaweeds. The technology for Porphyra cultivation in land-based ponds is readily available. This study corroborated previous know-how of Porphyra growth in tanks and ponds, and yet offers important improvements regarding seedling production and their handling for successful cultivation. This study supported various other activities opening additional important issues in the biology/cultivation/use of Porphyra and other seaweeds.
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Sela, Hanan, Eduard Akhunov, and Brian J. Steffenson. Population genomics, linkage disequilibrium and association mapping of stripe rust resistance genes in wild emmer wheat, Triticum turgidum ssp. dicoccoides. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7598170.bard.
Full textAbstract:
The primary goals of this project were: (1) development of a genetically characterized association panel of wild emmer for high resolution analysis of the genetic basis of complex traits; (2) characterization and mapping of genes and QTL for seedling and adult plant resistance to stripe rust in wild emmer populations; (3) characterization of LD patterns along wild emmer chromosomes; (4) elucidation of the multi-locus genetic structure of wild emmer populations and its correlation with geo-climatic variables at the collection sites. Introduction In recent years, Stripe (yellow) rust (Yr) caused by Pucciniastriiformis f. sp. tritici(PST) has become a major threat to wheat crops in many parts of the world. New races have overcome most of the known resistances. It is essential, therefore, that the search for new genes will continue, followed by their mapping by molecular markers and introgression into the elite varieties by marker-assisted selection (MAS). The reservoir of genes for disease and pest resistance in wild emmer wheat (Triticumdicoccoides) is an important resource that must be made available to wheat breeders. The majority of resistance genes that were introgressed so far in cultivated wheat are resistance (R) genes. These genes, though confering near-immunity from the seedling stage, are often overcome by the pathogen in a short period after being deployed over vast production areas. On the other hand, adult-plant resistance (APR) is usually more durable since it is, in many cases, polygenic and confers partial resistance that may put less selective pressure on the pathogen. In this project, we have screened a collection of 480 wild emmer accessions originating from Israel for APR and seedling resistance to PST. Seedling resistance was tested against one Israeli and 3 North American PST isolates. APR was tested on accessions that did not have seedling resistance. The APR screen was conducted in two fields in Israel and in one field in the USA over 3 years for a total of 11 replicates. We have found about 20 accessions that have moderate stripe rust APR with infection type (IT<5), and about 20 additional accessions that have novel seedling resistance (IT<3). We have genotyped the collection using genotyping by sequencing (GBS) and the 90K SNP chip array. GBS yielded a total 341K SNP that were filtered to 150K informative SNP. The 90K assay resulted in 11K informative SNP. We have conducted a genome-wide association scan (GWAS) and found one significant locus on 6BL ( -log p >5). Two novel loci were found for seedling resistance. Further investigation of the 6BL locus and the effect of Yr36 showed that the 6BL locus and the Yr36 have additive effect and that the presence of favorable alleles of both loci results in reduction of 2 grades in the IT score. To identify alleles conferring adaption to extreme climatic conditions, we have associated the patterns of genomic variation in wild emmer with historic climate data from the accessions’ collection sites. The analysis of population stratification revealed four genetically distinct groups of wild emmer accessions coinciding with their geographic distribution. Partitioning of genomic variance showed that geographic location and climate together explain 43% of SNPs among emmer accessions with 19% of SNPs affected by climatic factors. The top three bioclimatic factors driving SNP distribution were temperature seasonality, precipitation seasonality, and isothermality. Association mapping approaches revealed 57 SNPs associated with these bio-climatic variables. Out of 21 unique genomic regions controlling heading date variation, 10 (~50%) overlapped with SNPs showing significant association with at least one of the three bioclimatic variables. This result suggests that a substantial part of the genomic variation associated with local adaptation in wild emmer is driven by selection acting on loci regulating flowering. Conclusions: Wild emmer can serve as a good source for novel APR and seedling R genes for stripe rust resistance. APR for stripe rust is a complex trait conferred by several loci that may have an additive effect. GWAS is feasible in the wild emmer population, however, its detection power is limited. A panel of wild emmer tagged with more than 150K SNP is available for further GWAS of important traits. The insights gained by the bioclimatic-gentic associations should be taken into consideration when planning conservation strategies.
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Ward, Kimiora. Sierra Nevada Network high elevation white pine monitoring: 2021 annual report. National Park Service, 2024. http://dx.doi.org/10.36967/2302327.
Full textAbstract:
Five-needle white pines (Family Pinaceae, Genus Pinus, Subgenus Strobus), and in particular whitebark pine (Pinus albicaulis), limber pine (P. flexilis), and foxtail pine (P. balfouriana) are foundation species in upper subalpine and treeline forests of several National Park Service Pacific West Region parks, including Sequoia and Kings Canyon National Parks (SEKI) and Yosemite National Park (YOSE). The Sierra Nevada Network Inventory & Monitoring Program, in collaboration with the Klamath Network, Upper Columbia Basin Network, and Mojave Desert Network have implemented a joint long-term monitoring protocol to assess the current status and future trends in high elevation white pine communities. Key demographic parameters within white pine forest communities will be estimated by monitoring individual trees within permanent plots through time. This report documents the results of the 2021 field season, which was the eighth year of monitoring in Sequoia and Kings Canyon National Parks (SEKI) and Yosemite National Park (YOSE). The 2021 goal was to complete the third full re-measure of the second of three rotating panels (Panel 2) for each species-park population: YOSE-whitebark pine, SEKI-whitebark pine, and SEKI-foxtail pine. Each panel consists of 12 permanent 50 x 50 m (2,500 m2) plots that were randomly selected for each of the three populations. The full sampling array thus includes a total of 36 whitebark pine plots in YOSE, 36 whitebark pine plots in SEKI, and 36 foxtail pine plots in SEKI. Data from plot surveys will be used to characterize white pine forest community dynamics in SEKI and YOSE, including changes in tree species composition, forest structure, forest health, and demographics. The first full measure of all Panel 2 plots was completed over two years in 2013-2014, then a full remeasure of both parks? whitebark pine Panel 2 was conducted in 2016, with 10 of 12 SEKI-foxtail plots sampled that year. A third remeasure of all Panel 2 plots was not possible in 2021 because a smaller crew size was necessary during the COVID-19 pandemic. In total, the crew visited 37 sites, and sampled 31, during the 2021 field season. One plot in the YOSE whitebark pine frame was uninstalled before reading and one plot in the SEKI whitebark pine frame was uninstalled after reading, both for safety concerns. Four plots were not visited due to lack of capacity with the reduced crew size: one in each of the YOSE and SEKI whitebark frames, and three in the SEKI foxtail frame. A plot from Panel 3 in each of the parks? whitebark frames was measured, for a total of 11 plots measured in each whitebark pine frame. Nine plots were measured in the SEKI foxtail pine frame. Within the 31 plots completed, a total of 5,728 trees was measured. Species composition, forest structure, and factors affecting tree health and reproduction, including incidence and severity of white pine blister rust (Cronartium ribicola) infection, mountain pine beetle (Dendroctonus ponderosae) infestation, dwarf mistletoe (Arceuthobium spp.) infection, canopy kill, female cone production and regeneration were recorded. During the 2021 field season, crews continued to count the total number of mature cones per tree for whitebark and foxtail pine, use crown condition codes to assess crown health, and tag individual seedlings to be tracked through time. All three of these procedures started in 2017 and are to be evaluated by each of the three participating networks over several years, to determine whether they should become permanent changes to the monitoring protocol. In YOSE, 11 whitebark pine plots were re-measured, from Panels 2 and 3. A total of 2,810 trees were sampled, which included 586 live whitebark pine trees and 2,097 other live conifers. An additional 127 trees (including 17 whitebark pine) were recorded as dead. The forest crew noted little sign of white pine blister rust (WPBR) in Yosemite in 2021, and just a single inactive canker was observed on one whitebark pine in Panel 3, Plot 42, near Dana Meadows. This infection was new to plot 42, and it expands the total number of plots where white pine blister rust has been documented in Yosemite to six. The crew also noted little mountain pine beetle activity, documenting beetle galleries on 15 lodgepole pines in three Panel 2 plots. Dwarf mistletoe was not observed. The average number of live whitebark pine trees per plot was 53 (SD = 56). This was a low cone crop year for whitebark pine, with two percent of live whitebark pine trees producing female cones. Cone bearing trees averaged 2 (SD = 1) cones per tree. Whitebark pine seedling density averaged 90 (SD = 157) seedlings per hectare. The largest number of whitebark pine seedlings found in a plot was four, and three of the eleven plots contained whitebark seedlings. In SEKI, 10 of 12 Panel 2, and one Panel 3, whitebark pine plots were re-measured. Within these plots, 1,246 live whitebark pine, 30 live foxtail pine, and 861 other live conifers were sampled. WPBR was infrequently documented in the SEKI whitebark frame as well, with indicators of infection in Plot 31 near Window Creek and Plot 44 near Upper State Lake. These were the first infections documented in these plots, bringing the number of plots where WPBR has been documented in the SEKI whitebark panel to nine. Although WPBR was documented in Plot 27 near Charlotte Dome in 2016, it was not documented this year because putative cankers showing three signs of infection in 2016 showed only two or fewer signs in 2021. Mountain pine beetle activity was observed in one live lodgepole pine and two recently dead whitebark pine, within three plots in the SEKI whitebark sample frame. An exception to the low levels of mountain pine beetle activity was outside Plot 31 in the Window Creek area, where the forest crew noted many recently dead whitebark pine with signs of beetle activity. Dwarf mistletoe was not encountered. The average number of live whitebark pine trees per plot was 113 (SD = 86). Less than one percent of live whitebark pine trees produced female cones, each producing on average 2 (SD = 1) cones. Whitebark seedling regeneration averaged 303 (SD = 319) seedlings per hectare. The largest number of whitebark seedlings found in a plot was eight, and eight of the 11 plots contained whitebark seedlings. Nine of the 12 SEKI foxtail Panel 3 plots were remeasured. Within these plots, 413 live foxtail pine, 67 live whitebark pine, and 402 other live conifers were sampled. Ninety-two dead or recently dead trees were also documented, 65 of which were foxtail pine. No signs of blister rust infection, mistletoe, or mountain pine beetle were observed in the foxtail plots sampled. The average number of foxtail pine trees per plot was 46 (SD = 33). Fifty-four percent of the foxtail pine trees produced female cones, averaging 14 (SD =15) cones/tree. Only one foxtail pine seedling was recorded within the 9 foxtail pine plots, resulting in an estimated 14 (SD = 41) seedlings per hectare. Eight whitebark pine seedlings were also found within two plots.
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Moore, Gloria A., Gozal Ben-Hayyim, Charles L. Guy, and Doron Holland. Mapping Quantitative Trait Loci in the Woody Perennial Plant Genus Citrus. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570565.bard.
Full textAbstract:
As is true for all crops, production of Citrus fruit is limited by traits whose characteristics are the products of many genes (i.e. cold hardiness). In order to modify these traits by marker aided selection or molecular genetic techniques, it is first necessary to map the relevant genes. Mapping of quantitative trait loci (QTLs) in perennial plants has been extremely difficult, requiring large numbers of mature plants. Production of suitable mapping populations has been inhibited by aspects of reproductive biology (e.g. incompatibility, apomixis) and delayed by juvenility. New approaches promise to overcome some of these obstacles. The overall objective of this project was to determine whether QTLs for environmental stress tolerance could be effectively mapped in the perennial crop Citrus, using an extensive linkage map consisting of various types of molecular markers. Specific objectives were to: 1) Produce a highly saturated genetic linkage map of Citrus by continuing to place molecular markers of several types on the map. 2) Exploiting recently developed technology and already characterized parental types, determine whether QTLs governing cold acclimation can be mapped using very young seedling populations. 3) Determine whether the same strategy can be transferred to a different situation by mapping QTLs influencing Na+ and C1- exclusion (likely components of salinity tolerance) in the already characterized cross and in new alternative crosses. 4) Construct a YAC library of the citrus genome for future mapping and cloning.
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Ward, Kimiora. Sierra Nevada Network white pine monitoring: 2022 annual report. National Park Service, 2023. http://dx.doi.org/10.36967/2301003.
Full textAbstract:
Five-needle white pines (Family Pinaceae, Genus Pinus, Subgenus Strobus), and in particular whitebark pine (Pinus albicaulis), limber pine (P. flexilis), and foxtail pine (P. balfouriana) are foundation species in upper subalpine and treeline forests of several National Park Service Pacific West Region parks, including Sequoia and Kings Canyon National Parks (SEKI) and Yosemite National Park (YOSE). The Sierra Nevada Network Inventory & Monitoring Program, in collaboration with the Klamath Network, Upper Columbia Basin Network, and Mojave Desert Network have implemented a joint long-term monitoring protocol to assess the current status and future trends in high elevation white pine communities. Key demographic parameters within white pine forest communities will be estimated by monitoring individual trees within permanent plots through time. This report documents the results of the 2022 field season, which was the ninth year of monitoring in SEKI and YOSE. The 2021 goal was to complete the first full measure of the third of three rotating panels (Panel 3) for each species-park population: YOSE-whitebark pine, SEKI-whitebark pine, and SEKI-foxtail pine. Each panel consists of 12 permanent 50 x 50 m (2,500 m2) plots that were randomly selected for each of the three populations. The full sampling array thus includes a total of 36 whitebark pine plots in YOSE, 36 whitebark pine plots in SEKI, and 36 foxtail pine plots in SEKI. Data from plot surveys will be used to characterize white pine forest community dynamics in SEKI and YOSE, including changes in tree species composition, forest structure, forest health, and demographics. Partial measures of Panel 3 were completed in 2017 (11 plots) in Yosemite whitebark pine, in 2017 (9 plots) in SEKI whitebark pine, and in 2014 (7 plots) and 2017-2018 (8, 1 plots) in foxtail pine. In 2022, the first full measure of all Panel 3 plots (and 2nd or 3rd remeasure of most plots) was successfully completed, and installation was completed on four of these plots in SEKI whitebark pine and two in foxtail pine. In total, the crew visited 36 sites during the 2022 field season, all from Panel 3. Within the 36 completed Panel 1 plots, a total of 6,398 trees were measured. Species composition, forest structure, and factors affecting tree health and reproduction including incidence and severity of white pine blister rust (Cronartium ribicola) infection, mountain pine beetle (Dendroctonus ponderosae) infestation, dwarf mistletoe (Arceuthobium spp.) infection, canopy kill, and female cone production were recorded. During the 2022 field season crews continued to count the total number of mature cones per tree for whitebark and foxtail pine, use crown condition codes to assess crown health, and tag individual seedlings to be tracked through time. All three of these procedures started in 2017 and are to be evaluated by each of the three participating networks over several years, to determine whether they should become permanent changes to the monitoring protocol. In YOSE, all 12 Panel 3 whitebark pine plots were measured. A total of 2,720 trees were sampled, which included 977 live whitebark pine trees and 1,605 other live conifers. An additional 135 trees (including 26 whitebark) were recorded as dead. The average number of live whitebark pine trees per plot was 81 (SD = 94). White pine blister rust (WPBR) aecia were observed on five whitebark pine in one plot in YOSE in 2022, and no trees in any plot had inactive cankers showing three or more indicators of WPBR. WPBR had previously been documented in this plot, so the number of plots where rust has ever been observed in Yosemite remains unchanged at six. However, an infection documented in plot 42 in 2021 was not observed again when the plot was resampled in 2022, so it is possible this number should be five. Mountain pine beetle activity was observed on one live whitebark pine and three live and one dead lodgepole pine in YOSE in 2022. Despite documentation of many stands impacted by beetle attack in the field crew notes, the quantified rate of MPB attack was lower than in 2021. Twenty-one percent of live whitebark pine trees produced female cones. Cone-bearing trees averaged 7 (SD = 10) cones/tree. Whitebark pine seedling density averaged 80 (SD = 152) seedlings per hectare. The largest number of whitebark pine seedlings found in a plot was 51 and five of the twelve plots contained whitebark seedlings. All 12 Panel 3 SEKI whitebark pine plots were measured in 2022, and installation was completed on four of these, so this Panel is now fully installed. Within these plots, 2,179 live whitebark pine, 10 live foxtail pine, and 297 other live conifers were sampled (including 5 live western white pine). The average number of live whitebark pine trees per plot was 181 (SD = 125). Although the crew observed white pine blister rust in seven SEKI whitebark Panel 3 plots, no active cankers (aecia) were observed, and no trees displayed 3 of 5 indicators, so no infections were quantified. Mountain pine beetle activity was observed in 18 live and 23 dead whitebark pine and 1 live and one dead lodgepole pine within three plots in SEKI. Dwarf mistletoe was not encountered. Seven percent of live whitebark pine trees produced female cones. Cone-bearing trees averaged 3.7 (SD = 3.6) cones/tree. Whitebark seedling regeneration averaged 700 (SD = 752) seedlings per hectare. The largest number of whitebark seedlings found in a plot was 19, and two of the 12 plots did not contain any whitebark seedlings. In the foxtail pine Panel 3, all 12 plots were measured in 2022, and installation was completed on two of these, so installation of the panel is now complete. Within these plots we measured 309 live foxtail pine, 302 live whitebark pine, and 380 other live conifers, including four live western white pine. An additional 112 dead or recently dead trees and 22 unidentified snags were also measured, 19 of which were foxtail pine. The average number of foxtail pine trees per plot was 26 (SD = 26). No signs of blister rust infection or mistletoe were observed on foxtail pine. Mountain pine beetle activity was observed on one dead foxtail pine, one live whitebark pine, and seven live and one dead lodgepole pines within four plots. Sixty-two percent of the foxtail pine trees produced female cones. Cone-bearing trees averaged 33 (SD = 53) cones/tree. Seven foxtail pine seedlings were recorded within five plots, resulting in an estimated 72 (SD = 98) seedlings per hectare. Eight whitebark pine seedlings and three lodgepole pine seedlings were also found within three additional plots.
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Flaishman, Moshe, Herb Aldwinckle, Shulamit Manulis, and Mickael Malnoy. Efficient screening of antibacterial genes by juvenile phase free technology for developing resistance to fire blight in pear and apple trees. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7613881.bard.
Full textAbstract:
Objectives: The original objectives of this project were to: Produce juvenile-free pear and apple plants and examine their sensitivity to E. amylovora; Design novel vectors, for antibacterial proteins and promoters expression, combined with the antisense TFL1 gene, and transformation of Spadona pear in Israel and Galaxy apple in USA. The original objectives were revised from the development of novel vectors with antibacterial proteins combined with the TFL-1 due to the inefficiency of alternative markes initially evaluated in pear, phoshomannose-isomerase and 2-deoxyglucose-6-phosphate phosphatase and the lack of development of double selection system. The objectives of project were revised to focus primarily on the development additional juvenile free systems by the use of another pear variety and manipulation of the FT gene under the control of several promoters. Based on the results creation of fire blight resistance pear variety was developed by the use of the juvenile free transgenic plant. Background: Young tree seedlings are unable to initiate reproductive organs and require a long period of shoot maturation, known as juvenile phase. In pear, juvenile period can last 5-7 years and it causes a major delay in breeding programs. We isolated the TFL1 gene from Spadona pear (PcTFL1-1) and produced transgenic ‘Spadona’ trees silencing the PcTFL1 gene using a RNAi approach. Transgenic tissue culture ‘Spadona’ pear flowered in vitro. As expected, the expression of the endogenous PcTFL1 was suppressed in the transgenic line that showed precocious flowering. Transgenic plants were successfully rooted in the greenhouse and most of the plants flowered after only 4-8 months, whereas the non-transformed control plants have flowered only after 5-6 years of development. Major achievements: Prior to flower induction, transgenic TFL1-RNAi ‘Spadona’ plants developed a few branches and leaves. Flower production in the small trees suppressed the development of the vegetative branches, thus resulting in compact flowering trees. Flowering was initiated in terminal buds, as described for the Arabidopsis tfl1 mutant. Propagation of the transgenic TFL1-RNAi ‘Spadona’ was performed by bud grafting on 'Betulifolia' rootstock and resulted in compact flowering trees. The transgenic flowering grafted plants were grown in the greenhouse under a long photoperiod for one year, and flowered continuously. Pollination of the transgenic flowers with ‘Costia‘ pear pollen generated fruits of regular shape with fertile F1 seeds. The F1 transgenic seedling grown in the greenhouse formed shoots and produced terminal flowers only five months after germination. In addition, grafted F1 transgenic buds flower and fruit continuously, generating hybrid fruits with regular shape, color and taste. Several pear varieties were pollinated with the transgenic TFL1-RNAi ‘Spadona’ pollen including `Herald Harw` that was reported to have resistance to fire blight diseases. The F-1 hybrid seedlings currently grow in our greenhouse. We conclude that the juvenile-free transgenic ‘Spadona’ pear enables the development of a fast breeding method in pear that will enable us to generate a resistance pear to fire blight. Implications: The research supported by this grant has demonstrated the use of transgenic juvenile free technology in pear. The use of the juvenile free technology for enhancement of conventional breeding in fruit tree will serve to enhance fast breeding systems in pear and another fruit trees.
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Ohad, Nir, and Robert Fischer. Control of Fertilization-Independent Development by the FIE1 Gene. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575290.bard.
Full textAbstract:
A fundamental problem in biology is to understand how fertilization initiates reproductive development. During plant reproduction, one sperm cell fuses with the egg to form an embryo, whereas a second sperm cell fuses with the adjacent central cell nucleus to form the endosperm tissue that supports embryo and/or seedling development. To understand the mechanisms that initiate reproduction, we have isolated mutants of Arabidopsis that allow for replication of the central cell and subsequent endosperm development without fertilization. In this project we have cloned the MEA gene and showed that it encode a SET- domain polycomb protein. Such proteins are known to form chromatin-protein complexes that repress homeotic gene transcription and influence cell proliferation from Drosophylla to mammals. We propose a model whereby MEA and an additional polycomb protein we have cloned, FIE , function to suppress a critical aspect of early plant reproduction and endosperm development, until fertilization occurs. Using a molecular approach we were able to determine that FIE and MEA interact physically, suggesting that these proteins have been conserved also during the evolution of flowering plants. The analysis of MEA expression pattern revealed that it is an imprinted gene that displays parent-of- origin-dependent monoallelic expression specifically in the endosperm tissue. Silencing of the paternal MEA allele in the endosperm and the phenotype of mutant mea seeds support the parental conflict theory for the evolution of imprinting in plants and mammals. These results contribute new information on the initiation of endosperm development and provide a unique entry point to study asexual reproduction and apomixis which is expected to improve crop production.
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VanderGheynst, Jean, Michael Raviv, Jim Stapleton, and Dror Minz. Effect of Combined Solarization and in Solum Compost Decomposition on Soil Health. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7594388.bard.
Full textAbstract:
In soil solarization, moist soil is covered with a transparent plastic film, resulting in passive solar heating which inactivates soil-borne pathogen/weed propagules. Although solarization is an effective alternative to soil fumigation and chemical pesticide application, it is not widely used due to its long duration, which coincides with the growing season of some crops, thereby causing a loss of income. The basis of this project was that solarization of amended soil would be utilized more widely if growers could adopt the practice without losing production. In this research we examined three factors expected to contribute to greater utilization of solarization: 1) investigation of techniques that increase soil temperature, thereby reducing the time required for solarization; 2) development and validation of predictive soil heating models to enable informed decisions regarding soil and solarization management that accommodate the crop production cycle, and 3) elucidation of the contributions of microbial activity and microbial community structure to soil heating during solarization. Laboratory studies and a field trial were performed to determine heat generation in soil amended with compost during solarization. Respiration was measured in amended soil samples prior to and following solarization as a function of soil depth. Additionally, phytotoxicity was estimated through measurement of germination and early growth of lettuce seedlings in greenhouse assays, and samples were subjected to 16S ribosomal RNA gene sequencing to characterize microbial communities. Amendment of soil with 10% (g/g) compost containing 16.9 mg CO2/g dry weight organic carbon resulted in soil temperatures that were 2oC to 4oC higher than soil alone. Approximately 85% of total organic carbon within the amended soil was exhausted during 22 days of solarization. There was no significant difference in residual respiration with soil depth down to 17.4 cm. Although freshly amended soil proved highly inhibitory to lettuce seed germination and seedling growth, phytotoxicity was not detected in solarized amended soil after 22 days of field solarization. The sequencing data obtained from field samples revealed similar microbial species richness and evenness in both solarized amended and non-amended soil. However, amendment led to enrichment of a community different from that of non-amended soil after solarization. Moreover, community structure varied by soil depth in solarized soil. Coupled with temperature data from soil during solarization, community data highlighted how thermal gradients in soil influence community structure and indicated microorganisms that may contribute to increased soil heating during solarization. Reliable predictive tools are necessary to characterize the solarization process and to minimize the opportunity cost incurred by farmers due to growing season abbreviation, however, current models do not accurately predict temperatures for soils with internal heat generation associated with the microbial breakdown of the soil amendment. To address the need for a more robust model, a first-order source term was developed to model the internal heat source during amended soil solarization. This source term was then incorporated into an existing “soil only” model and validated against data collected from amended soil field trials. The expanded model outperformed both the existing stable-soil model and a constant source term model, predicting daily peak temperatures to within 0.1°C during the critical first week of solarization. Overall the results suggest that amendment of soil with compost prior to solarization may be of value in agricultural soil disinfestations operations, however additional work is needed to determine the effects of soil type and organic matter source on efficacy. Furthermore, models can be developed to predict soil temperature during solarization, however, additional work is needed to couple heat transfer models with pathogen and weed inactivation models to better estimate solarization duration necessary for disinfestation.
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Newton, Ronald, Joseph Riov, and John Cairney. Isolation and Functional Analysis of Drought-Induced Genes in Pinus. United States Department of Agriculture, 1993. http://dx.doi.org/10.32747/1993.7568752.bard.
Full textAbstract:
Drought is a common factor limiting timber production in the U.S. and Israel. Loblolly (Pinus taeda) and alleppo pine (Pinus halepensis) seedling survival is reduced when out planted, and growth and reproduction are often hindered by periodic droughts during later stages of tree development. Molecular and gene responses to drought stress have not been characterized. The objectives were to characterize drought-induced gene clones from these pines, to determine the effects of a growth regulator on drought tolerance, ABA levels, and drought-induced gene expression in alleppo pine, and to develop procedures for loblolly pine transformation. Nearly 20 cDNA clones influenced by gradual, prolonged drought stress have been isolated. Many of these have been shown to be induced by drought stress, whereas several others are down-regulated. These are the first drought-induced genes isolated from a pine species. Two genomic clones (lp5-1 and lp3-1) have been sequenced and characterized, and each has been found to be associated with a gene family. Clone lp5 appears to code for a cell wall protein, and clone lp3 codes for a nuclear protein. The former may be associated with changing the elastic properties of the cell wall, while the latter may be involved in signal transduction and/or protection from desiccation in the nucleus. Clone lp3 is similar to a drought-induced gene from tomato and is regulated by ABA. Several DNA sequences that are specific to induction during growth-retardation in alleppo pine by uniconazole have been identified. The active DNA species is now being identified. Promoters from genomic clones, lp3 and lp5, have been sequenced. Both are functional when fused with the gus reporter gene and transferred to other plant tissues as well as responding to a simulated drought stress. Through exodeletion analysis, it has been established that the promoter ABRE element of lp3 responds to ABA and that drought-induction of lp3 expression may also involve ABA. Stable tobacco transformants carrying either the lp5 or the lp3 promoter fused to a reporter gus gene have been obtained. The lp5lgus fusion was expressed at several stages of tobacco development and differentiation including the reproductive stage. There was no difference in phenotype between the transformants and the wild type. Embryogenesis procedures were developed for slash pine, but attempts to couple this process with gene transfer and plantlet transformation were not successful. Transformation of pine using Agrobacterium appears tractable, but molecular data supporting stable integration of the Agrobacterium-transferred gene are still inconclusive.
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Shelton, Michael G. Effects of seed production, seedbed condition, and overstory basal area on the establishment of shortleaf pine seedlings in the Ouachita Mountains. U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station, 1995. http://dx.doi.org/10.2737/so-rp-293.
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