Academic literature on the topic 'Seedling Response'
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Journal articles on the topic "Seedling Response"
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Islam, Sumaiya, Md Nasim Reza, Shahriar Ahmed, et al. "Seedling Growth Stress Quantification Based on Environmental Factors Using Sensor Fusion and Image Processing." Horticulturae 10, no. 2 (2024): 186. http://dx.doi.org/10.3390/horticulturae10020186.
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Understanding the diverse environmental influences on seedling growth is critical for maximizing yields. The need for a more comprehensive understanding of how various environmental factors affect seedling growth is required. Integrating sensor data and image processing techniques offers a promising approach to accurately detect stress symptoms and uncover hidden patterns, enhancing the comprehension of seedling responses to environmental factors. The objective of this study was to quantify environmental stress symptoms for six seedling varieties using image-extracted feature characteristics. Three sensors were used: an RGB camera for color, shape, and size information; a thermal camera for measuring canopy temperature; and a depth camera for providing seedling height from the image-extracted features. Six seedling varieties were grown under controlled conditions, with variations in temperature, light intensity, nutrients, and water supply, while daily automated imaging was conducted for two weeks. Key seedling features, including leaf area, leaf color, seedling height, and canopy temperature, were derived through image processing techniques. These features were then employed to quantify stress symptoms for each seedling type. The analysis of stress effects on the six seedling varieties revealed distinct responses to environmental stressors. Integration of color, size, and shape parameters established a visual hierarchy: pepper and pak choi seedlings showed a good response, cucumber seedlings showed a milder response, and lettuce and tomato seedlings displayed an intermediate response. Pepper and tomato seedlings exhibited a wide range of growth stress symptoms, at 13.00% to 83.33% and 2.96% to 70.01%, respectively, indicating considerable variability in their reactions to environmental stressors. The suggested classification approach provides valuable groundwork for advancing stress monitoring and enabling growers to optimize environmental conditions.
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Landhäusser, Simon M., Erin T. Wiley, Kevin A. Solarik, Shaun P. Kulbaba, and Alexander E. Goeppel. "The Importance of Initial Seedling Characteristics in Controlling Allocation to Growth and Reserves under Different Soil Moisture Conditions." Forests 14, no. 4 (2023): 796. http://dx.doi.org/10.3390/f14040796.
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After disturbance, forest regeneration and resiliency depend on the ability of seedlings to respond, survive, and grow under a variety of stress conditions, including drought. Despite recent efforts to improve our fundamental knowledge surrounding plant response mechanisms to stress and their application in seedling quality research, initial seedling characteristics are often ignored when exploring seedling responses to stress in field plantings or ecophysiological studies. Here, we explore how initial differences in size, biomass allocation, and non-structural carbohydrate (NSC) storage affect the subsequent partitioning of new biomass, growth potential, and drought response in seedlings of a deciduous broad-leaved (Populus tremuloides) and an evergreen coniferous species (Pinus banksiana). We exposed seedlings of both species to different growing conditions in their first growing season in order to manipulate the aforementioned seedling characteristics. In a second growing season, we exposed these different seedling types to a subsequent drought stress. While drought reduced both structural growth and NSC storage in all seedling types, the expected shift in allocation favoring roots was only observed in seedling types with initially low root:shoot or root:stem ratios. Overall, we also found that the traits associated with greater growth were quite different between pine and aspen. While larger seedlings led to greater growth in pine, it was the smallest seedling type in aspen with the largest root:stem ratio that produced the most new growth. In aspen, this smaller seedling type was the only one that did not undergo a shift in biomass relative to its initial allometry, suggesting that adjustments in biomass allocation made by other, larger seedling types must have come at the cost of lower growth. In contrast, adjustments in allocation did not appear to negatively impact pine, possibly because reduced root:shoot ratios of larger seedlings did not reduce NSC storage, as it did in aspen. Our results highlight (1) the complexity of how differences in biomass allocation and changes in seedling size may alter storage and the response of species to drought, and (2) the importance of accounting for initial seedling characteristics (both morphological and physiological) when predicting seedling growth and the impacts of environmental stressors.
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Moore, James A., Zhaofei Fan, and Bahman Shafii. "Effect of Root-Plug Incorporated Controlled-Release Fertilizer on Two-Year Growth and Survival of Planted Ponderosa Pine Seedlings." Western Journal of Applied Forestry 17, no. 4 (2002): 216–19. http://dx.doi.org/10.1093/wjaf/17.4.216.
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Abstract Three controlled-release fertilizers (fast release [FR], moderate release [MR], and slow release [SR]) were incorporated in the root plug at rates of 0.8, 1.6, or 3.2 g/seedling at the time of sowing as supplements to nursery supplied soluble fertilizer. Effects on seedling growth, survival, and foliar nutrient status of the “160/90” container ponderosa pine (Pinus ponderosa) were evaluated after outplanting. At the end of the second growing season, fertilized seedlings had significantly greater diameter and height than unfertilized seedlings. The 3.2 g of MR or SR fertilizer treatments produced significantly higher mortality (55 and 36%, respectively) than the controls. The fast release fertilizer included at a rate of 0.8 g in each seedling's container was the preferred treatment since it produced good survival and seedling growth response. A 2-yr growth response of about 25% was similar to that observed in a nearby study using adjacent placement of controlled-release fertilizer after planting ponderosa pine seedlings. West. J. Appl. For. 17(4):216–219.
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Sayar, R., H. Bchini, M. Mosbahi, and H. Khemira. "Response of durum wheat (Triticum durum Desf.) growth to salt and drought stresses." Czech Journal of Genetics and Plant Breeding 46, No. 2 (2010): 54–63. http://dx.doi.org/10.17221/85/2009-cjgpb.
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Two durum wheat (Triticum durum Desf.) cultivars were tested for salt and drought tolerance at germination, seedling emergence and early seedling growth in NaCl and PEG-8000 solutions of different osmotic potentials (–0.2, –0.4, –0.6 and –0.8 MPa). Daily and final germination and emergence percentage, as well as germination and seedling emergence rate, seedling growth, fresh and dry weight were recorded under controlled conditions. Results showed that germination and emergence rates were delayed by both solutions in both cultivars, but Omrabia showed higher germination and emergence rates than BD290273 in NaCl while BD290273 was less affected by NaCl and PEG solutions at the emergence stage. Sodium chloride had a lesser effect on both cultivars in terms of germination rate, emergence rate, final germination and emergence percentage than did PEG-8000. This conclusively proves that the adverse effect of PEG-8000 on germination, emergence and early seedling growth was due to the osmotic effect rather than to the specific ion. Seedling growth was reduced by both stresses. However, NaCl usually caused less damage than PEG to durum wheat seedlings, suggesting that NaCl and PEG acted through different mechanisms.
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Ross, Darrell W., and C. Wayne Berisford. "Nantucket Pine Tip Moth (Lepidoptera: Tortricidae) Response to Water and Nutrient Status of Loblolly Pine." Forest Science 36, no. 3 (1990): 719–33. http://dx.doi.org/10.1093/forestscience/36.3.719.
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Abstract Loblolly pine (Pinus taeda L.) seedlings were grown in a greenhouse under conditions of variable water and nutrient availability and then exposed to natural populations of the Nantucket pine tip moth (Rhyacionia frustrana [Comstock]). Seedling growth was directly related to water and nutrient availability. Tip moth oviposition was highest on the most vigorous seedlings, even after accounting for differences in seedling size among treatments. The ratios of mean number of pupae per seedling to mean number of eggs per seedling for each treatment indicated percent tip moth survival may have been lower on vigorous seedlings than on stressed seedlings, but differences may have been due to factors other than the suitability of seedlings as larval food. Larger numbers of heavier pupae developed on vigorous seedlings than on stressed seedlings. Pupal weights were directly related to total nitrogen concentration and inversely related to total phenolic and condensed tannin concentrations of pine shoot tissues. Total phenolic and condensed tannin concentrations were inversely related to seedling growth. These results suggest that tip moth populations will increase rapidly following silvicultural treatments that increase water and nutrients available to young loblolly pines. For. Sci. 36(3):719-733.
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O'Neill, G. A., C. P. Chanway, P. E. Axelrood, R. A. Radley, and F. B. Holl. "An assessment of spruce growth response specificity after inoculation with coexistent rhizosphere bacteria." Canadian Journal of Botany 70, no. 12 (1992): 2347–53. http://dx.doi.org/10.1139/b92-294.
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The influence of inoculation with rhizosphere bacteria on hybrid spruce (Picea glauca × engelmannii) seedling growth was investigated by isolating bacteria from the rhizosphere of naturally regenerating spruce seedlings collected from two ecologically distinct zones of British Columbia. Forty bacterial strains from spruce were screened in a seedling growth experiment, and several strains were shown to stimulate spruce seedling growth. The three most effective spruce growth promoting strains from each ecological zone were then tested in a seedling growth response specificity experiment in which seed, soil, and bacteria from both spruce ecosystems were examined in all possible treatment combinations, including pasteurized soil. Spruce seedling growth was primarily affected by seed and soil source (Salmon Arm > Mackenzie for both factors), and by soil pasteurization (pasteurized > unpasteurized). Significant growth effects due to bacterial treatment in the specificity experiment occurred only in pasteurized soil, but seedling biomass accumulation was stimulated by up to 59% in response to inoculation. There was no evidence of growth response specificity that was related to plant – bacteria adaptation within spruce ecotypes; two of the Mackenzie bacterial strains significantly inhibited growth of Mackenzie seedlings in Mackenzie soil, but two strains stimulated the growth of Salmon Arm seedlings, one in each soil type. Two Salmon Arm strains significantly stimulated growth of Mackenzie seedlings on Salmon Arm soil, and two strains stimulated the growth of Salmon Arm seedlings, one in each soil type. Seedling biomass was greater when treatments of spruce seed, soil, and rhizosphere bacteria that originated from the same geographic site were pooled and compared with treatments in which at least one factor originated from the other site. However, this effect was explained by the significant seed × soil interaction in the absence of bacteria; mean seedling biomass was greatest when spruce seed and soil treatments with the same geographic origin were pooled and compared with unrelated seed – soil combinations. Seedlings from pooled treatments were also significantly larger when bacteria and soil had a common geographic origin, but only when tested in pasteurized soil. Our results indicate that spruce growth promoting rhizosphere bacteria can be isolated from naturally regenerating spruce seedlings, but growth response variability was observed between trials. Seedling growth promotion by bacterial strains was not related to the use of spruce ecotypes, soil, and (or) rhizosphere bacteria with a common geographic origin. Key words: spruce seedlings, growth, inoculation, rhizosphere bacteria.
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Edelman, Nichole F., and Michelle L. Jones. "Evaluating Ethylene Sensitivity within the Family Solanaceae at Different Developmental Stages." HortScience 49, no. 5 (2014): 628–36. http://dx.doi.org/10.21273/hortsci.49.5.628.
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The family Solanaceae, which includes both important crop and ornamental species, is generally considered to have high sensitivity to ethylene. Our objectives were to evaluate ethylene sensitivity between accessions with the family Solanaceae and to determine whether similar sensitivity was observed in seedlings and mature plants. For the seedling evaluations, seeds were germinated and grown in the dark on filter paper saturated with 0 or 100 μM 1-aminocyclopropane-1-carboxylic acid (ACC; the immediate precursor to ethylene). The relative hypocotyl length at 100 μM ACC was compared with untreated control (0 μM) seedlings. Mature plants were treated with 0 or 10 μL·L−1 ethylene in the dark for 24 hours. Ethylene responses including flower abscission, flower senescence, and epinasty were observed and quantified. Seedlings and mature plants were classified as having no response, low, medium, or high ethylene sensitivity based on the severity of the ethylene responses observed. Sensitivity differences were observed among seedling, juvenile, and mature plants, and a range of ethylene responses and symptom severity was observed between accessions within a species. The majority of the accessions were classified as medium or high ethylene sensitivity at both the seedling and mature plant stages. Solanum melongena ‘Black Beauty’ (eggplant) had a low response to ethylene at the seedling stage and a high response at the mature plant stage, whereas Petunia ×hybrida ‘Daddy Orchid’ had a high response at the seedling stage and a low response at the mature plant stage. Peppers (Capsicum annum), tomatoes (Solanum lycopersicum), and tomatillos (Physalis ixocarpa) exhibited both floral and vegetative symptoms of ethylene damage, whereas calibrachoas (Calibrachoa ×hybrida), eggplants, nicotianas, and petunias exhibited only floral symptoms. The most common floral response to ethylene treatment was flower abscission, which was observed in almost all of the Solanum, Capsicum, and Nicotiana accessions. We consistently observed ethylene-induced epinasty in the genus Capsicum and in all of the Solanum except eggplant. Our results indicated that developmental stage influenced ethylene sensitivity, and there was not a consistent correlation between seedling and mature plant responses within the Solanaceae accessions that we evaluated.
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Frisby, James W., and Schuyler D. Seeley. "CHILLING OF PEACH SEEDS, SEEDLINGS AND CUTTINGS." HortScience 25, no. 9 (1990): 1088f—1088. http://dx.doi.org/10.21273/hortsci.25.9.1088f.
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Correlations were made between the responses of seeds, physiologically dwarfed seedlings and dormant cuttings to similar chilling treatments. Seed germination correlated highly with growth of physiologically dwarfed seedlings and shoot growth of dormant cuttings. Emergence and seedling growth correlated poorly with germination, growth of physiologically dwarfed seedlings and shoot growth of dormant cuttings. Thus, germination was a better seed predictor of the mature peach chilling response than emergence or seedling growth. Growth of dwarfed seedlings correlated highly with shoot growth of dormant cuttings. The anomalous leaf condition of peach seedlings may have confounded seedling growth after seed chilling, but was not a problem when the chilling treatment was provided to physiologically dwarfed seedlings. The dormancy release mechanisms that promoted seed germination, growth of physiologically dwarfed seedlings and growth of dormant cuttings were similar.
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Al-Din, N. S., and A. H. Alalaf. "Response of Seedlings of Two Olive Varieties to the Addition of Chemical and Biological Fertilization." IOP Conference Series: Earth and Environmental Science 1449, no. 1 (2025): 012137. https://doi.org/10.1088/1755-1315/1449/1/012137.
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Abstract Nursery owners suffer from slow growth of olive seedlings, and thus production costs increase until they are ready for planting in a sustainable orchard. Therefore, this study aimed to add biological and chemical fertilization to seedlings of two types of olives for the purpose of improving their growth and mineral content. The experiment included two factors, the first nine fertilizer treatments containing 15gm. Seedling−1NPK (20:20:20), 10g.Seedling−1NPK+5g.Seedling−1 Biofertilizer Gorabac G, 5g.Seedling−1NPK+10g.Seedling−1 Biofertilizer Gorabac G, 2.5g.Seedling−1NPK +15g. Seedlings−1 Biofertilizer Gorabac G, 20g. Seedlings−1 Biofertilizer Gorabac G, 10g. Seedlings−1NPK+5ml.Seedlings−1Biofertilizer in broth form, 5g.Seedlings−1NPK+10ml.Seedlings−1 Biofertilizer In broth form, 2.5 gm. Seedling−1 NPK + 15 ml. Seedling−1 Biofertilizer in broth form, 20 ml. Seedling−1 biofertilizer in broth form. The second factor included two olive varieties (Nabali and Sourani), according to the results measured at the end of the experiment. It was found that there were no significant differences between the factors for the (leaf content of phosphorus, potassium, nitrogen, protein, and dry weight of the root system). On the other hand, the treatment 10 g. seedling−1 NPK + 5 ml. seedling−1 biofertilizer in broth form was superior to the rest of the treatments in terms of dry weight of the shoot, as well. The Nabali variety was significantly superior to the Sorani variety in the values of this trait.
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Singh, Nidhi, A. Saxena, and R. Singh. "Growth responses of Jatropha Curcas seedlings under different soil mixtures, fertilizer doses, irrigation regimes and sodicity levels." Journal of Non-Timber Forest Products 17, no. 2 (2010): 163–71. http://dx.doi.org/10.54207/bsmps2000-2010-05mv72.
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The present study analyses the growth responses of Jatropha curcas seedlings under different soil mixtures, fertilizer doses, irrigation frequency and soil sodicity levels. Among all the soil mixtures, seedlings of J. curcas indicated higher growth, dry weight and quality index in the mixture of soil, sand and FYM in 1:2: 2 and 1: 1: 2 ratios. Further, increasing FYM in all the combinations of soil mixture increased the seedling growth. However, increase of sand in the soil mixture beyond 1: 2: 2 ratios of soil, sand and FYM did not improve the seedling growth. The application of NPK fertilizers in soil @ 100: 75: 75 mg per seedling showed maximum growth, dry weight and quality index of seedlings. It was also observed that application of N in higher doses reduced the seedling growth and dry weight. The seedlings of this species relatively performed better up to low moisture stress level than under intermediate and high moisture stress levels. Root dry weight and root: shoot ratio, however, increased with increasing the moisture stress levels. Under various soil sodicity levels, the response of J. curcas seedlings indicated a marked reduction in growth and dry weight with increasing levels of sodicity. Further, the response breadths were comparatively lower under sodicity levels than under soil mixture, fertilizer doses and moisture stress levels.
Dissertations / Theses on the topic "Seedling Response"
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Edelman, Nichole Francis. "Evaluating Ethylene Sensitivity Using Mature Plant Screens and the Seedling Hypocotyl Response." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1385569998.
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Hill, Stephanie R. "Evaluation of seed and seedling response to aid revegetation of hazardous chemical waste sites." Thesis, Virginia Tech, 1993. http://hdl.handle.net/10919/40671.
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The response of several plant species to heavy metal
contaminated soils was evaluated using plant bioassays with
a soil substrate. A natural soil was collected from
Dinwiddie County, Virginia and soil analysis was performed.
The plant species, Lolium multiflorum, setaria italica and
Trifolium rep ens latum, Robinia pseudoacacia, Andropogon
gerardi, Asclepias syriaca, Echinacea purpurea, Rudbeckia
hirta and Festuca rubra were grown in to determine the
response to cupric and cadmium chloride in soils (mg Cu/kg
soil). A few plant species were grown in small pots in a
plant growth chamber for 28 days using control, 10, 30, 100
and 300mg Cu or Cd/kg soil. Germination proved to be less
sensitive than root length. S. italica had highest ECSOs.
In eu 20.7 and 15.3 in Cd. All plant species were grown
for 7 days in 0.3, 1.0, 3.0 10.0, and 30.0mg Cu/kg soil and
in control. Germination was not effected by metal
concentrations in most species (p=0.07-0.6), except
T.repens latum, R. hirta and F. rubra at 30mg/kg
(p=0.0007). Root length was significantly effected by Cu
concentrations for almost all species (p=0.0001-0.0112).
Setaria italica had the highest EC50 at 10.86mg/kg. Robinia
pseudoacacia root length was not significantly affected
by CU concentrations. The other species had EC50s ranging
from 3.74-7.51mg/kg. Both inhibition and stimulation of
root growth were observed.
<p>Preliminary studies regarding germination rates,
fungicides and rangefinding are included.<br>Master of Science
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Yang, Dongsheng. "The response of two eucalypt subspecies to water stress and fertilizer at early seedling stage." Thesis, Canberra, ACT : The Australian National University, 1987. http://hdl.handle.net/1885/140223.
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Jones, Trevor A. "The effects of seed size on seedling growth response to elevated CO¦2 in four conifer species." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ62346.pdf.
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Liu, Rui. "Regulation and molecular signaling during seed germination and seedling establishment of arabidopsis in response to abiotic stresses." HKBU Institutional Repository, 2013. https://repository.hkbu.edu.hk/etd_oa/101.
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Plants regulate many physiological processes in response to adverse environmental stresses. This study focused on the seed germination and seedling establishment stage and investigated the molecular signaling events when abiotic stresses, such as osmotic, water and temperature, were applied. Seeds of Arabidopsis, mutants or wild type, were used to identify the signaling components. Cold-pretreatment (stratification) is widely used to break seed dormancy and improve germination rate. Stratification at 4. significantly broke the seed dormancy of Arabidopsis in wild-type, cyp707a2, sleepy1 and sleepy1/cyp707a2, but not in ga3ox1. Stratification and exogenous ABA treatment strongly enhanced the expression and the activity of a-amylase in the freshly harvested seeds among the wild-type and those mutants, which have relatively high ABA content. Similarly, the expression of RGL2 and ABI5 were also substantially suppressed by stratification. These results suggest that stratification firstly leads to GA biosynthesis and unlocks the inhibition of RGL2 on the expression of a-amylase. Stratification also relieves the inhibition of ABA on the germination process but the inhibition of ABA on seedling development is not affected. We have isolated an Arabidopsis mutant, dsptp1, which is hyposensitive to osmotic stress during seed germination and seedling establishment, indicated by exhibiting higher seed germination rate, lower inhibition in root elongation under osmotic stress, and more tolerance to drought compared with the wild type (Col0) plants. Osmotic stress and drought enhanced AtDsPTP1 expression in seed coats, the bases of rosette leaves and roots. Compared with the wild type, the dsptp1 mutant increased proline accumulation, reduced MDA content and ion leakage, and enhanced antioxidant enzyme activity under osmotic stress. AtDsPTP1 regulated the transcript levels of various dehydration responsive genes, ABA biosynthesis and metabolic enzyme gene under osmotic stress, resulting in reduced accumulation of ABA in dsptp1 mutant plants than wild type in response to osmotic stress. AtDsPTP1 also mediated the ABA signaling pathway under osmotic stress by suppressing the expression of ABI1 and enhancing the expression of the positive regulators ABI3 and ABI5 in ABA signaling. These data suggest that AtDsPTP1 positively regulates ABA accumulation and signaling during seed germination and seedling establishment in Arabidopsis under osmotic stress. To further investigate the regulation mechanism of DsPTP1 in osmotic stress and drought signaling, we analyzed the water holding capacity between wild type and dsptp1 mutant. The dsptp1 mutant exhibited enhanced water holding capacity compared to wild type under osmotic stress resulting from reduced water loss and increased relative water content, which shall contribute the osmotic and drought tolerance. To identify the signaling components, we investigated the activity of MAPKs under osmotic and drought stress and found that the DsPTP1 differentially regulates the activities of MAPK6 and a p38 MAPK, which is inferred as MAPK12 according to its molecular weight in Arabidopsis under osmotic and salt stress. However, there is no direct interaction between DsPTP1 and 20 MAPKs indicated by the results of the of specific interaction test. These results suggest that the differential regulation of MAPK6 and MAPK12 by DsPTP1 is indirect. In addition, we screened the interaction proteins of DsPTP1 under abiotic stress. Seventeen positive clones were acquired from the sequencing results. More work need to be done to confirmed the positive interactions and the signaling cascades. In summary, seed germination and seedling growth are closely regulated by environmental cues. This should be the result of evolutionary selection since successful new growth from the seed embryo depends on the sensitive perception of environmental conditions and effective regulation of many physiological processes that are involved. We have demonstrated that plant hormones, especially ABA, play central regulative roles during such regulations. Many other signaling components, such as protein kinases and phosphatases, are also involved. Identifying the detailed signaling pathways should be the focus of further research.
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Nkomo, Mbukeni Andrew. "The role of p-coumaric acid on physiological and biochemical response of chia seedling under salt stress." University of the Western Cape, 2020. http://hdl.handle.net/11394/7954.
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Philosophiae Doctor - PhD<br>The role of phenolic acids in mitigating salt stress tolerance have been well documented. However, there are contradicting reports on the effect of exogenously applied phenolic acids on the growth and development of various plants species. A general trend was observed where phenolic acids were shown to inhibit plant growth and development, with the exception of a few documented cases. One of these such cases is presented in this thesis. This study investigates the role of exogenously applied p-coumaric acid (p-CA) on physio-biochemical and molecular responses of chia seedlings under salt stress. This study is divided into three parts. Part one (Chapter 3) focuses on the impact of exogenous p-coumaric acid on the growth and development of chia seedlings. In this section, chia seedlings were supplemented with exogenous p-CA and the various biochemical and plant growth parameters were measured. The results showed that exogenous p-CA enhanced the growth of chia seedlings. An increase in chlorophyll, proline and superoxide oxide contents were also observed in the p-CA treatment relative to the control. We suggested that the increase in chia seedling growth could possibly be via the activation of reactive oxygen species-signalling pathway involving O2− under the control of proline accumulation (Chapter 3). Given the allopathy, nature of p-coumaric acid it is noteworthy that the response observed in this study may be species dependent, as contrasting responses have been reported in other plant species. Part two (Chapter 4) of this study investigates the influence of piperonylic acid (an inhibitor of endogenous p-coumaric acid) on the growth and development of chia seedlings.
In trying to illustrate whether p-CA does play a regulatory role in enhancing pseudocereal plant growth, we treated chia seedlings with the irreversible inhibitor of C4H enzyme, to inhibit the biosynthesis of endogenous p-CA. In this section, chia seedlings were treated with piperonylic acid and changes in plant growth, ROS-induced oxidative damage, p-CA content and antioxidant capacity was monitored. Inhibition of endogenous p-CA restricted chia seedling growth by enhancing ROS-induced oxidative damage as seen for increased levels of superoxide, hydrogen peroxide and the extent of lipid peroxidation. Although an increase in antioxidant activity was observed in response to piperonylic acid, this increase was not sufficient to scavenge the ROS molecules to prevent oxidative damage and ultimate cellular death manifested as reduced plant growth. The results presented in this section support our hypothesis that p-CA play an important regulatory role in enhancing chia seedling growth and development as shown in Chapter 3. Part three (Chapter 5) seeks to identify and functionally characterise p-coumaric acid induced putative protein biomarkers under salt stress conditions in chia seedlings.
Previous studies have shown that p-CA reversing the negative effect caused by NaCl-induced salt stress. While these studies were able to demonstrate the involvement of p-CA in promoting plant growth under salt stress conditions, they focussed primarily on the physiological aspect, which lacks in-depth biochemical and molecular analysis (ionomic and proteomic data) which could help in detecting the genes/proteins involved in salt stress tolerance mechanisms. A comparative ionomics and proteomic study was conducted, with the aim of elucidating the pivotal roles of essential macro elements and/or key protein markers involved in p-CA induced salt stress tolerance in chia seedlings. With the exception of Na, all the other macro elements were decreased in the salt treatment. Contrary to what was observed for the salt treatment most of the macro elements were increased in the p-CA treatment. However, the addition of exogenous p-CA to salt stressed seedlings showed an increase in essential macro elements such as Mg and Ca which have been shown to play a key role in plant growth and development. In the proteomic analysis we identified 907 proteins associated with shoots across all treatments. Interestingly, only eight proteins were conserved amongst all treatments. A total of 79 proteins were unique to the p-CA, 26 to the combination treatment (NaCl + p-CA) and only two proteins were unique to the salt stress treatment. The unique proteins identified in each of the treatments were functionally characterised to various subcellular compartments and biological processes. Most of the positively identified proteins were localised to the chloroplast and plays key roles in photosynthesis, transportation, stress responses and signal transduction pathways. Moreover, the protein biomarkers identified in this study (especially in the p-CA treatment) are putative candidates for genetic improvement of salt stress tolerance in plants.
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Hosseini, Mohammad Khajeh. "The response of soybean seeds to the stresses of semi-arid environments during germination and early seedling growth." Thesis, University of Aberdeen, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324912.
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Reduced water availability and salinity are two major environmental factors influencing crop establishment in semi-arid environments. Therefore the effect of reduced water availability using polyethylene glycol (PEG) 4000 solutions and of salinity (NaCl) on the germination of six soybean cultivars was examined. Cultivars differed in their response to reduced water availability and salinity and in their ability to recover from the stresses. A large increase in germination during a recovery period at 0 MPa following water stress suggested that PEG was not toxic whilst the failure of seeds to recover from high salinity revealed the toxic effects of NaCl. At the same water potential, germination in saline conditions was higher than that in PEG and the rate of water uptake by individual seeds was more rapid in NaCl solutions than in PEG. The most plausible explanation for the greater water uptake and germination in NaCl is that seeds accumulated salts which lowered their osmotic potential. The effects of NaCl on seedling growth were much greater when experiments were conducted in a hydroponic system compared with a paper towel method. However, analysis of the solutions soaking the paper towels revealed that 4.25<I> </I>mMolal Ca2<sup>+</sup> was available to the seeds in this system in saline conditions. This may have reduced Na<sup>+</sup> uptake or provided a protective effect against Na<sup>+</sup> toxicity. Germination (40%) was possible at a tissue Na<sup>+</sup> concentration in the embryonic axis of 9.3mg g-1 FW whilst seedling growth was completely inhibited at a tissue Na<sup>+</sup> concentration of 6.1 mg g<sup>-1 </sup>FW. Germination at higher tissue Na<sup>+</sup> concentrations was associated with higher K<sup>+</sup>+Ca2<sup>+</sup> concentrations, suggesting that these ions may protect the seeds in the pregermination phase against salinity. A reduction in seed vigour due to ageing resulted in reduced germination under saline conditions compared to the germination of unaged seeds, but there was no significant interaction between salinity and seed ageing. However, unaged seeds showed a greater increase in germination after transfer to 0 MPa than did the aged seeds. Since both the site of ageing and the toxic effect of NaCl is the cell membrane, there may be additive effects of NaCl toxicity on cell membrane in aged seeds.
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Knee, Emma M. "Use of photomorphogenic and hormone-response mutants to investigate the role of hormones in light regulation of seedling development /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487936356158079.
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Mann, Rebecca K. "Intraspecific Variation in the Response of Elymus Elymoides to Competition from Bromus Tectorum." DigitalCommons@USU, 2016. https://digitalcommons.usu.edu/etd/4749.
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Native plant materials are often seeded to restore biodiversity and ecosystem function in areas overtaken by exotic weeds. Plant materials are evaluated on intraspecific differences in productivity and expression of traits advantageous to establishment (e.g., phenology, seed mass, and growth rate); some investigations also consider population-level adaptation to exotic species. However, there is a lack of studies that broadly evaluate response to competition from exotic species at multiple scales within a species.
In a greenhouse experiment, we used analysis of variance to assess the growth response of a perennial grass native to the Intermountain West, (Elymus elymoides), to competition from a common invasive species, (Bromus tectorum), at three levels of intraspecific differentiation: subspecies, lineage (wild vs. domestic germplasm), and population. We used regression analysis to assess whether E. elymoides populations from highly invaded areas were less affected by B. tectorum competition. Finally, we explored the relationship between growth traits and competitive response using random forest regression. We found significant differences among E. elymoides subspecies in their response to B. tectorum competition, no difference between wild and domestic lineages, and no population-level differentiation within subspecies. Field abundance of B. tectorum had a significant positive relationship with E. elymoides biomass, but not competitive response, suggesting that E. elymoides has not adapted to the invader. Elymus elymoides plants which were less affected by competition were smaller, allocated more biomass to leaves, and had fewer fine roots, suggesting that light interception and tissue retention were prioritized by seedlings in this competitive greenhouse environment.
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Phang, Ing Chia. "Response of Arabidopsis thaliana seedlings to lead exposure." Thesis, University of Canterbury. School of Biological Sciences, 2010. http://hdl.handle.net/10092/4467.
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Lead (Pb) is one of the most commonly occurring, highly persistent and widely distributed heavy metal contaminants in the environment. It has a tendency to bioaccumulate in animals and plants, and potentially, it is able to enter the human food chain where it poses a hazard to public health. Generally, conventional remediation technologies applied to decontaminate heavy metals from groundwater and soils are very costly. Hence, phytoremediation has emerged as an ecologically friendly and economically attractive technology that uses green plants to clean up heavy metal contaminated sites. However, a lack of knowledge of the biological processes associated with plant responses to Pb (e.g. Pb uptake, accumulation, translocation, and tolerance) has been a bottleneck for the application of Pb phytoremediation in the field. A model genetic system of higher plants, Arabidopsis thaliana, was selected to further examine the physiological, biochemical and molecular events occuring in plants under Pb stress.
The overall aim of this project was to obtain a better understanding of plant responses to Pb contaminants in the early developmental stages of A. thaliana seedlings. This research encompassed the physiological responses of A. thaliana seedlings to Pb exposure, monitoring their antioxidative defence systems, and investigating the participation of annexin 1 in the response to Pb-mediated oxidative stress. This research also assessed the protective effect of nitric oxide on Pb-induced toxicity of A. thaliana seedlings and it isolated a putative Pb tolerant mutant from an EMS-mutagenized M2 population. A multiexperimental approach was adopted to achieve these objectives.
A. thaliana seedlings were grown on modified Huang & Cunningham (1996) nutrient solution containing 0.8% (w/v) agar, with and without Pb(NO3)2, under controlled conditions. A. thaliana seedlings were insensitive to Pb during seed germination. In treatments with up to 200 μM Pb(NO₃)₂, morphological changes and inhibition of root growth were observed in the 7-d-old seedlings. A tolerance index revealed that Pb(NO₃)₂ concentration of 75 μM and higher brought about more than 50% root growth inhibition. Pb was predominantly retained in the roots. Analysis using a graphite furnace atomic absorption spectroscopy indicated that the level of Pb accumulation in A. thaliana roots was greatly dependent on the Pb(NO₃)₂ concentrations, but only a small fraction of the accumulated Pb was translocated to the shoots (18 - 43%). Transmission electron microscopy analysis showed that Pb was mainly immobilized in the cell walls and intercellular spaces. This was interpreted as a mechanism that minimizes the entry of Pb into cells and interference with cellular functions. Pb that gained entry into the cytoplasm was sequestered into the vacuoles.
The toxicity of Pb in the cytosol of A. thaliana seedlings was studied by measuring the H₂O₂ and lipid hydroperoxide levels using a microplate reader. When the Pb(NO₃)₂ concentration in the growth medium was 100 μM, the 7-d-old seedlings contained 2.2-fold higher H₂O₂ and 9.6-fold higher lipid hydroperoxide than the control without Pb(NO₃)₂. This was followed by an up-regulation of the activity of antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPX), and general peroxidase (POD) by 2.1-, 3.2-, 2.3-, 1.8- and 4.6-fold, respectively, compared with the control. Pb toxicity is known to trigger oxidative stress, but A. thaliana seedlings appeared to be capable of activating cell rescue, defending themselves against harmful oxidative stress and also acclimating to Pb. Data from physiological and biochemical analysis indicate that a combination of avoidance and tolerance mechanisms exists in Pb-treated A. thaliana seedlings to maintain the essential cellular metabolism for survival.
Real-time reverse-transcription polymerase chain reaction was used to show the involvement of AnnAt1 in the response of 7-d-old A. thaliana seedlings to a high threshold concentration of Pb. When the seedlings were treated with 100 μM Pb(NO₃)₂, AnnAt1 message levels were up-regulated by 2.12-fold. Pb-mediated oxidative stress may be a component of AnnAt1 gene expression. AnnAt1 potentially could be invoked to reduce the toxic effects of Pb stress by acting as ROS and/or Ca²⁺ signals, as a membrane protector, in detoxification of excessive ROS, or in sequestration of Pb.
Pb stress symptoms were less evident in seedlings pre-treated with 1 mM sodium nitroprusside (SNP), a nitric oxide (NO) donor. The present study found that exogenous NO did not alter Pb transport into the plants or efflux pumping of Pb at the plasma membrane. However, NO conferred protection to 7-d-old A. thaliana seedlings primarily by acting as an antioxidant or a signal for actions to scavenge excessive ROS level. The application of exogenous NO before subjecting to 100 μM Pb(NO₃)₂ decreased H₂O₂ back to its original level, and reduced 50% lipid hydroperoxide in the Pb-treated seedlings. As a result, the antioxidative enzyme activities in Pb-exposed seedlings pre-treated with SNP were 23 - 45% lower than those without SNP pre-treatment. Less antioxidative enzyme activities were probably needed to counteract the reduced amount of Pb-induced ROS in A. thaliana seedlings.
A post-germination procedure involving prolonged exposure to 150 μM Pb(NO₃)₂ was developed to screen an EMS-mutagenized M2 population of A. thaliana. Potential Pb tolerant mutants were selected based on the ability to grow with their roots penetrating into the medium and maintain purple-green leaves without wilting. A minority of the survivors appeared to go into a resting stage and they seemed to have altered transporters that prevented Pb from entering the cells. Only one putative Pb mutant (M3-1) was recovered from the rescue and set seeds. The M₄ generation of this putative Pb mutant was re-screened for phenotypic confirmation and to determine the regulation of AnnAt1. The 7-d-old putative Pb mutant seemed to display enhanced root and shoot growth in the presence of 150 μM Pb(NO₃)₂ compared to the wild-type seedlings. The transcript level of AnnAt1 in this putative Pb tolerant mutant increased by 2.19-fold when exposed to 150 μM Pb(NO₃)₂.
Books on the topic "Seedling Response"
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F, Newman Reg, and British Columbia. Ministry of Forests. Research Branch., eds. Response of lodgepole pine seedlings to simulated cattle damage. British Columbia Ministry of Forests, Research Program, 1997.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1985.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1985.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Forest and Range Experiment Station, 1985.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1985.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1985.
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Radwan, M. A. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Dept. of Agriculture, Forest Service, Pacific Northwest Research Station, 1985.
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Rahmaningsih, M., I. Virdiana, S. Bahri, Y. Anwar, B. P. Forster, and F. Breton, eds. Nursery screening for iGanoderma/i response in oil palm seedlings: a manual. CABI, 2018. http://dx.doi.org/10.1079/9781786396242.0000.
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Fleming, R. L. Establishment and growth of seeded upland black spruce: 7-12 year response. Great Lakes Forestry Centre, 1995.
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Colombo, S. J. Response of containerized black spruce and jack pine seedlings to fertilization rate and growing medium. Ontario Tree Improvement and Forest Biomass Institute, Ministry of Natural Resources, 1988.
Find full textBook chapters on the topic "Seedling Response"
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Yadukrishnan, Premachandran, Deeksha Singh, Nevedha Ravindran, et al. "Hormones and Light-Regulated Seedling Development." In Hormones and Plant Response. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-77477-6_4.
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Jack, Steven B., and Stephen D. Pecot. "Regeneration Dynamics, Competition, and Seedling Response." In Ecological Restoration and Management of Longleaf Pine Forests. CRC Press, 2017. http://dx.doi.org/10.1201/9781315152141-4.
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Rundel, P. W., G. A. Baker, D. J. Parsons, and T. J. Stohlgren. "Postfire demography of resprouting and seedling establishment by Adenostoma fasciculatum in the California chaparral." In Plant Response to Stress. Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-70868-8_40.
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Huston, Michael A., Milena Holmgren, and Michelle B. Kreh. "Response of Understory Tree Seedling Populations to Spatiotemporal Variation in Soil Moisture." In Ecological Studies. Springer New York, 2003. http://dx.doi.org/10.1007/978-1-4613-0021-2_14.
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Siregar, I. Z., and H. H. Hattemer. "Gene flow and mating system in a seedling seed orchard and a natural stand of Pinus merkusii Jungh. et de Vriese in Indonesia." In Genetic Response of Forest Systems to Changing Environmental Conditions. Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9839-2_23.
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Geras'kin, Stanislav, Roman Churyukin, Polina Volkova, and Sofiya Bitarishvili. "Using ionizing radiation for improving the development and yield of agricultural crops." In Mutation breeding, genetic diversity and crop adaptation to climate change. CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0043.
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Abstract The response of barley seedlings was studied after gamma irradiation of seeds with doses in the range of 2-50 Gy. It was shown that stimulation of plant growth occurred in the dose range of 16-20 Gy. The influences of the dose rate, the quality of seeds and their moisture on the manifestation of radiation effects were investigated. We studied, under controlled conditions, the activities of metabolic and antioxidant enzymes and observed an increase in their activity in the range of doses that cause stimulation of seedling growth. We showed that changes in the balance among different classes of phytohormones were probably involved in the acceleration of plant growth after irradiation of seeds using stimulating doses. Gamma irradiation of barley seeds significantly influenced the development of plants during the growing season. After irradiation with stimulating doses, we observed a reduction in the duration of the initial stages of ontogenesis; the phase of full ripeness occurred 5-7 days earlier than in the controls. The manifestation of the effect of irradiation depended on the conditions in which the plants developed. During the growing season of 2014, which was a dry year, plants originating from the irradiated seeds showed an increase in the number of productive stems, which led to an increase in yield by 34-38%; during the optimal 2015 season, an increase in the number of grains per spike caused an increase in yield by 8-29%. Therefore, our field study has shown that at least some hormetic effects can occur in the field. Irradiation of seeds can increase field germination, stimulate the growth and development of plants and increase their resistance to unfavourable environmental conditions. A more complete understanding of the underlying mechanisms of hormesis is needed to exploit its potential benefits in crop production.
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Nehring, Ramlah B., and Joseph R. Ecker. "Ethylene Responses in Seedling Growth and Development." In Plant Hormones. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-1-4020-2686-7_17.
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Jentschke, Georg, and Douglas L. Godbold. "Metal Toxicity in Mycorrhizal Norway Spruce Seedlings." In Responses of Forest Ecosystems to Environmental Changes. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2866-7_141.
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Jentschke, Georg, and Douglas L. Godbold. "Metal Toxicity in Mycorrhizal Norway Spruce Seedlings." In Responses of Forest Ecosystems to Environmental Changes. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2866-7_169.
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Thellier, Michel. "Discovery of the Existence of Memory in Bidens Seedlings." In Plant Responses to Environmental Stimuli. Springer Netherlands, 2017. http://dx.doi.org/10.1007/978-94-024-1047-1_3.
Full textConference papers on the topic "Seedling Response"
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Ma, Baodong, Ao Xu, Xuanxuan Zhang, and Lixin Wu. "Chlorophyll change and spectral response of maize seedling under iron stress." In 2016 IEEE International Geoscience and Remote Sensing Symposium (IGARSS). IEEE, 2016. http://dx.doi.org/10.1109/igarss.2016.7730672.
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Zhou, T. T., L. Xue, and Z. M. Wang. "Photosynthetic response of greening seedling of four tree species to low temperature stress." In 2015 International Conference on Food Hygiene, Agriculture and Animal Science. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789813100374_0024.
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Li, Xiaoying, and Bo Zhang. "Improving Edamame Seedling Establishment by Determining the Optimal Temperature." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/ybzg7716.
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Demand in the United States for edamame has increased over the past two decades. Edamame has become the second largest soyfood consumed in the US (25,000-30,000 tons annually). While domestic edamame production is slowly increasing, at least 70% of edamame consumed in the US is imported. Poor crop establishment is a major problem limiting edamame production. Seedling emergence ranges from 50-85%, which is lower than that of grain-type soybean ( >85%). Seedling stand can be improved by growing under the optimal temperature. Up to now, edamame is planted still based on grain-type soybean recommendations. The objective of this study was to determine the minimum, optimum, and maximum emergence temperatures of edamame. It will provide a scientific basis for deciding the best planting time of edamame, ultimately improving its seedling stand in the field. In this study, two edamame cultivars (VT Sweet and UA Kirksey) were tested for their seedling emergence percentage and emergence rate (i.e., emergence speed) at 5, 7, 10, 13, 16, 19, 21, 23, 25, 27, 29, 32, 35, 38, 41, 45 °C (soil temperature) using the LabFieldTM Simulation Table. Two grain-type soybean cultivars (MFS-48P1 and USF5618v) were checks. The results showed that the optimal temperature range for edamame seedling emergence is 25-32 °C, which is lower than that of grain-type soybean (29-36 °C). Soybean did not emerge at around 4 °C, however, edamame seeds may have a higher tolerance to low temperature compared with grain-type soybean. The maximum temperature varied little among soybean types, with an average of 41.08 °C. In addition, edamame needs more thermal time to emerge. The results indicate that edamame has a different emergence response to temperature compared with grain-type soybean. Edamame should be planted earlier than grain-type soybean and later planting may reduce edamame seedling emergence.
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Marii, Liliana, Larisa Andronic, Marionela Sahanovschih, and Angela Ionascu-Urechii. "The effects of high temperature at different ontogenetic stages on some phenotypic indices in tomato plants." In Conferința științifică națională cu participare internațională "Integrare prin cercetare și inovare", dedicată Zilei Internaționale a Științei pentru Pace și Dezvoltare. Moldova State University, 2025. https://doi.org/10.59295/spd2024n.17.
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The study aims to investigate the tomato plants response to heat stress applied at the germination and seedling stages, evaluating certain phenotypic parameters in order to highlight possible particularities associated with adaptability to unfavorable environmental conditions. The research included three tomato genotypes with determined stress reactions that were designed into four variants: optimal-optimal, optimal-stress, stress-optimal, and stress-stress. The application of high temperatures at the germination stage significantly affects the subsequent development of tomato plants, resulting in a decrease in the average values of morphological traits compared to optimal conditions variants. The effects of heat stress vary depending on the genotype and the analyzed trait, with certain variants exhibiting specific vulnerabilities. Variance analysis shows that the temperature conditions and the stress application scheme significantly contribute to the variability of morphological traits, with stress at the germination stage having a dominant impact on architectural traits of plants, while genotype becomes the major influencing factor at the seedling stage. The results emphasize the importance of plant adaptability to thermal stress at different developmental stages.
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Refli and Yekti Asih Purwestri. "The response of antioxidant genes in rice (Oryza sativa L.) seedling Cv. Cempo Ireng under drought and salinity stresses." In TOWARDS THE SUSTAINABLE USE OF BIODIVERSITY IN A CHANGING ENVIRONMENT: FROM BASIC TO APPLIED RESEARCH: Proceeding of the 4th International Conference on Biological Science. Author(s), 2016. http://dx.doi.org/10.1063/1.4953521.
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Kartika, Elis, Lizawati Lizawati, Made Deviani Duaja, and Gusniwati Gusniwati. "The Response of Some Rubber Seedling (Hevea brasiliensis) Clones on Various Types of Indigenous Mycorrhizae in Ex-Coal Mined Soil Media." In The 3rd Green Development International Conference (GDIC 2020). Atlantis Press, 2021. http://dx.doi.org/10.2991/aer.k.210825.002.
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Kreis, Kevin F., and Sangjin Ryu. "Automated Mini-Channel Platform for Studying Plant Root Environments." In ASME 2021 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/fedsm2021-65493.
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Abstract Plants are crucial to our lives because they provide us with building materials, oxygen, and food. A season’s crop yield can be significantly affected by local environmental factors. In particular, improving fundamental understanding of plant root interactions with their local soil environment, or rhizosphere, will help improve crop yield. Studying such interactions is challenging because roots are underground, making it difficult to observe interactions and to manipulate the local soil environment. The goal of this study was to develop an automated mini-channel platform to investigate how plant roots respond to changes in their environment using corn as a model plant. Considering the size of corn seedling roots, mini-channel devices were fabricated in soft lithography using master molds produced with a 3D printer and polydimethylsiloxane (PDMS). Our use of a 3D printer instead of photolithography allowed for a broader range of PDMS mold designs, such as including embedded rubber gaskets built into the mold. Then, corn seedlings were grown inside the transparent mini-channel devices, and they were found to consume an observable amount of nitrate over time. Image processing was employed to measure the contour length of the roots for quantitative characterization of root growth. Then, an automated platform was developed to measure the growth rate of the corn seedling roots and the consumed nitrate over time. The automated platform maintained the level of growth medium in the channel device, and was equipped with a digital camera to image the root growing in the channel, electrochemical sensors to measure changes in nitrate concentration in the channel, and sensors to measure temperature and humidity. Therefore, the platform could automatically measure root growth while simultaneously measuring root environment. The platform’s adaptable design, simple fabrication, and low cost make it simple to replicate and use to study different plants and environmental stimuli.
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Yuan, XZ, D. Liang, X. Wang, and H. Xia. "Kiwifruit Seedlings 'Watt' and 'Hayward' Physiological Response to Salt Stress." In Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018). Atlantis Press, 2018. http://dx.doi.org/10.2991/icammce-18.2018.31.
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Gorczyca, Anna, Ewa Pociecha, and Ewelina Matras. "Response of Pea Seedlings on Multi-Walled Carbon Nanotubes Seed Treatments." In The 8th World Congress on New Technologies. Avestia Publishing, 2022. http://dx.doi.org/10.11159/icepr22.168.
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Černiauskas, Valentinas, and Iveta Varnagirytė-Kabašinskienė. "Evaluating early changes in young tree seedlings under simulated urban environment." In Research for Rural Development 2024 : annual 30th international scientific conference. Latvia University of Life Sciences and Technologies, 2024. https://doi.org/10.22616/rrd.30.2024.046.
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Urban trees, integral to urban environments, demonstrate intricate responses to atmospheric pollutants like particulate matter (PM), tropospheric ozone (O3), and carbon dioxide (CO2). Notably, O3 induces oxidative stress in leaf tissues, while PM, consisting of fine airborne particles, interacts with urban trees through foliar deposition. This interaction is particularly interesting as tree canopies are highly effective filters, capturing and accumulating PM on their surfaces. The present study focused on silver birch, small-leaved lime, and Norway maple seedlings responses to elevated O3 and CO2 with and without PM. Maple seedlings exhibited the highest stem height increment, followed by lime and birch. Elevated O3 and CO2 without PM led to substantial height increments for lime and maple. Elevated O3 and CO2 without PM increased the total polyphenols in lime and maple leaves but decreased the content of total flavonoids in birch and lime leaves. Our findings underscore the adaptability of lime and maple seedlings to elevated O3 and CO2, positioning them as promising species for urban environments in the face of changing climates. Birch, while exhibiting biochemical changes, demonstrated less pronounced growth responses. This studyʼs insights into the intricate interactions between urban trees and multiple pollutants, particularly the species-specific responses, are of significant value for urban planning and environmental management.
Reports on the topic "Seedling Response"
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Guy, Charles, Gozal Ben-Hayyim, Gloria Moore, Doron Holland, and Yuval Eshdat. Common Mechanisms of Response to the Stresses of High Salinity and Low Temperature and Genetic Mapping of Stress Tolerance Loci in Citrus. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613013.bard.
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The objectives that were outlined in our original proposal have largely been achieved or will be so by the end of the project in February 1995 with one exception; that of mapping cold tolerance loci based on the segregation of tolerance in the BC1 progeny population. Briefly, our goals were to 1) construct a densely populated linkage map of the citrus genome: 2) map loci important in cold and/or salt stress tolerance; and 3) characterize the expression of genes responsive to cold land salt stress. As can be seen by the preceding listing of accomplishments, our original objectives A and B have been realized, objective C has been partially tested, objective D has been completed, and work on objectives E and F will be completed by the end of 1995. Although we have yet to map any loci that contribute to an ability of citrus to maintain growth when irrigated with saline water, our very encouraging results from the 1993 experiment provides us with considerable hope that 1994's much more comprehensive and better controlled experiment will yield the desired results once the data has been fully analyzed. Part of our optimism derives from the findings that loci for growth are closely linked with loci associated with foliar Cl- and Na+ accumulation patterns under non-salinization conditions. In the 1994 experiment, if ion exclusion or sequestration traits are segregating in the population, the experimental design will permit their resolution. Our fortunes with respect to cold tolerance is another situation. In three attempts to quantitatively characterize cold tolerance as an LT50, the results have been too variable and the incremental differences between sensitive and tolerant too small to use for mapping. To adequately determine the LT50 requires many plants, many more than we have been able to generate in the time and space available by making cuttings from small greenhouse-grown stock plants. As it has turned out, with citrus, to prepare enough plants needed to be successful in this objective would have required extensive facilities for both growing and testing hardiness which simply were not available at University of Florida. The large populations necessary to overcome the variability we encountered was unanticipated and unforeseeable at the project's outset. In spite of the setbacks, this project, when it is finally complete will be exceedingly successful. Listing of Accomplishments During the funded interval we have accomplished the following objectives: Developed a reasonably high density linkage map for citrus - mapped the loci for two cold responsive genes that were cloned from Poncirus - mapped the loci for csa, the salt responsive gene for glutathione peroxidase, and ccr a circadian rhythm gene from citrus - identified loci that confer parental derived specific DNA methylation patterns in the Citrus X Poncirus cross - mapped 5 loci that determine shoot vigor - mapped 2 loci that influence leaf Na+ accumulation patterns under non-saline conditions in the BC1 population - mapped 3 loci that influence leaf Na+ accumulation paterns during salt sress - mapped 2 loci that control leaf Cl- accumulation patterns under non-saline conditions - mapped a locus that controls leaf Cl- accumulation patterns during salt stress Screened the BC1 population for growth reduction during salinization (controls and salinized), and cold tolerance - determined population variation for shoot/root ratio of Na+ and Cl- - determined levels for 12 inorganic nutrient elements in an effort to examine the influence of salinization on ion content with emphasis on foliar responses - collected data on ion distribution to reveal patterns of exclusion/sequestration/ accumulation - analyzed relationships between ion content and growth Characterization of gene expression in response to salt or cold stress - cloned the gene for the salt responsive protein csa, identified it as glutathione peroxidase, determined the potential target substrate from enzymatic studies - cloned two other genes responsive to salt stress, one for the citrus homologue of a Lea5, and the other for an "oleosin" like gene - cold regulated (cor) genes belonging to five hybridization classes were isolated from Poncirus, two belonged to the group 2 Lea superfamily of stress proteins, the others show no significant homology to other known sequences - the expression of csa during cold acclimation was examined, and the expression of some of the cor genes were examined in response to salt stress - the influence of salinization on cold tolerance has been examined with seedling populations - conducted protein blot studies for expression of cold stress proteins during salt stress and vice versa
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Radwan, M. A., and J. S. Shumway. Response of Douglas-fir seedlings to nitrogen, sulfur, and phosphorus fertilizers. U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station, 1985. http://dx.doi.org/10.2737/pnw-rp-346.
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Leis, Sherry, and Mary Short. Vegetation community monitoring at Pea Ridge National Military Park, Arkansas: 2007–2021. National Park Service, 2023. http://dx.doi.org/10.36967/2299454.
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Resource managers at Pea Ridge National Military Park manage the natural communities of the park as a backdrop for interpreting the civil war battle that occurred on March 7–8, 1862. Restoration of the landscape to the vegetation communities that were present at the time of the battle is ongoing. Priorities for restoration include density, form, and vegetation structure, but native representative species are also desired. Heartland Inventory and Monitoring Network ecologists observed plant community sites in park woodlands in 2007, 2012, 2016, and 2021. Climate may influence vegetation and other park natural resources. Temperatures have been increasing at the park, but precipitation and drought indices did not demonstrate significant trends. There was a great degree of interannual variability in precipitation and drought metrics. Phenological data indicated earlier first bloom and leaf-out dates. Overstory canopy, basal area, density, and tree stocking were similar through time, but class 1, midstory trees increased in basal area and density after 2007. The overstory structure remained that of a closed woodland despite prescribed fires and cedar thinning that occurred at the park. Our monitoring data show that fire management goals for overstory reduction have not yet been met. Ground cover was similar through time except for bare soil and deciduous leaf litter. Bare soil and leaf litter experienced a pulse in variability in 2012 and 2021, presumably in response to heterogeneous prescribed fires. Maintaining heterogeneous ground cover may best support biodiversity across the landscape. Ground flora cover (excluding tree regeneration) increased by 88% from 2007 to 2021, meeting a fire management goal. However, ground flora cover was heterogeneous across the sites. Concomitant with the increase in ground flora cover, we observed notable increases in alpha diversity (mean site species richness) and gamma diversity (parkwide species richness) across the monitoring period. Although increases may have been related to treatments and environmental factors, we also improved our botanical sampling preparation and included an expert botanist on the crew in 2021. Species composition of the ground flora was assessed via guilds and indicator species. Although tree regeneration was not included in estimates of total ground flora cover, it was highly variable through time. The seedling class comprised the majority of the regeneration stems observed. Although mean small sapling density values increased by 535% over 2007 levels, there was a great deal of variability among the sites, indicating small sapling estimates were contained within the confidence intervals and not truly different through time. Forbs comprised the greatest abundance of the ground flora guilds through time, except in 2012. Forbs also exhibited the greatest variability in all years except for 2012. Woody species increased over our monitoring record but remained low in cover. We analyzed a set of 50 indicator species for Ozark highlands woodlands to understand changes in the ground flora. The number of woodland indicator species observed in each monitoring event was low (ranged from 7–14 species) with the highest number of species observed in 2021. We also calculated invasive species metrics and found the number of invasive species increased from 2 to 11 over the monitoring record. Nepalese browntop (Microstegium vimineum) was the most abundant of these species. Our confidence in the 2021 ground flora observations was high. We found our observer error to be within standard levels, including agreement on species cover estimates. Not all sites have received the number of burns described in the park’s ecological fire management goals. Our monitoring data show that one of the fire management goals, increased ground flora cover, has been met, but the other two goals for overstory density reduction and seedling density have not yet been met. Further analysis of species composition of the overstory and tree regeneration will be needed after the fire treatments have been completed to determine if all fire management goals are being met.
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Houpis, J. L. J., K. A. Surano, S. Cowles, M. P. Costella, S. E. Benes, and P. D. Anderson. Comparison of the response of mature branches and seedlings of Pinus ponderosa to atmospheric pollution: Annual report. Office of Scientific and Technical Information (OSTI), 1989. http://dx.doi.org/10.2172/6323922.
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Chamovitz, Daniel, and Albrecht Von Arnim. Translational regulation and light signal transduction in plants: the link between eIF3 and the COP9 signalosome. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7696515.bard.
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The COP9 signalosome (CSN) is an eight-subunit protein complex that is highly conserved among eukaryotes. Genetic analysis of the signalosome in the plant model species Arabidopsis thaliana has shown that the signalosome is a repressor of light dependent seedling development as mutant Arabidopsis seedlings that lack this complex develop in complete darkness as if exposed to light. These mutant plants die following the seedling stage, even when exposed to light, indicating that the COP9 signalosome also has a central role in the regulation of normal photomorphogenic development. The biochemical mode of action of the signalosome and its position in eukaryotic cell signaling pathways is a matter of controversy and ongoing investigation, and recent results place the CSN at the juncture of kinase signaling pathways and ubiquitin-mediated protein degradation. We have shown that one of the many CSN functions may relate to the regulation of translation through the interaction of the CSN with its related complex, eukaryotic initiation factor (eIF3). While we have established a physical connection between eIF3 subunits and CSN subunits, the physiological and developmental significance of this interaction is still unknown. In an effort to understand the biochemical activity of the signalosome, and its role in regulating translation, we originally proposed to dissect the contribution of "h" subunit of eIF3 (eIF3h) along the following specific aims: (i) Isolation and phenotypic characterization of an Arabidopsis loss-of-function allele for eIF3h from insertional mutagenesis libraries; (ii) Creation of designed gain and loss of function alleles for eIF3h on the basis of its nucleocytoplasmic distribution and its yeast-two-hybrid interactions with other eIF3 and signalosome partner proteins; (iii) Determining the contribution of eIF3h and its interaction with the signalosome by expressing specific mutants of eIF3h in the eIF3h- loss-of function background. During the course of the research, these goals were modified to include examining the genetic interaction between csn and eif3h mutations. More importantly, we extended our effort toward the genetic analysis of mutations in the eIF3e subunit, which also interacts with the CSN. Through the course of this research program we have made several critical scientific discoveries, all concerned with the apparent diametrically opposed roles of eIF3h and eIF3e. We showed that: 1) While eIF3e is essential for growth and development, eIF3h is not essential for growth or basal translation; 2) While eIF3e has a negative role in translational regulation, eIF3h is positively required for efficient translation of transcripts with complex 5' UTR sequences; 3) Over-accumulation of eIF3e and loss-of-function of eIF3h both lead to cop phenotypes in dark-grown seedlings. These results were published in one publication (Kim et al., Plant Cell 2004) and in a second manuscript currently in revision for Embo J. Are results have led to a paradigm shift in translation research – eIF3 is now viewed in all systems as a dynamic entity that contains regulatory subuits that affect translational efficiency. In the long-term agronomic outlook, the proposed research has implications that may be far reaching. Many important plant processes, including developmental and physiological responses to light, abiotic stress, photosynthate, and hormones operate in part by modulating protein translation [23, 24, 40, 75]. Translational regulation is slowly coming of age as a mechanism for regulating foreign gene expression in plants, beginning with translational enhancers [84, 85] and more recently, coordinating the expression of multiple transgenes using internal ribosome entry sites. Our contribution to understanding the molecular mode of action of a protein complex as fundamental as eIF3 is likely to lead to advances that will be applicable in the foreseeable future.
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Houpis, J. L. J., D. J. Anschel, J. C. Pushnik, and P. D. Anderson. Variability in the intraspecific response of Pinus ponderosa seedlings subjected to long-term exposure to elevated CO{sub 2}. Office of Scientific and Technical Information (OSTI), 1995. http://dx.doi.org/10.2172/52786.
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Houpis, J. L. J., P. D. Anderson, S. E. Benes, S. P. Phelps, and A. T. Loeffler. Progress report for the project: Comparison of the response of mature branches and seedlings of Pinus ponderosa to atmospheric pollution. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/6481193.
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Chamovitz, Daniel A., and Albrecht G. Von Arnim. eIF3 Complexes and the eIF3e Subunit in Arabidopsis Development and Translation Initiation. United States Department of Agriculture, 2009. http://dx.doi.org/10.32747/2009.7696545.bard.
Full textAbstract:
The original working hypothesis of our proposal was that The “e” subunit of eIF3 has multiple functions from both within the nucleus and in the cytoplasm. Within this model, we further hypothesized that the “e” subunit of eIF3 functions in translation as a repressor. We proposed to test these hypotheses along the following specific aims: 1) Determine the subcellular localization of the interaction between eIF3e and other eIF3 subunits, or the COP9 signalosome. 2) Elucidate the biological significance of the varied subcellular localizations of eIF3e through generating Arabidopsis eIF3e alleles with altered subcellular localization. 3.) Purify different eIF3e complexes by tandem affinity purification (TAP). 4) Study the role of eIF3e in translational repression using both in vitro and in planta assays. eIF3 is an evolutionarily ancient and essential component of the translational apparatus in both the plant and animal kingdoms. eIF3 is the largest, and in some ways the most mysterious, of the translation factors. It is a multi-subunit protein complex that has a structural/scaffolding role in translation initiation. However, despite years of study, only recently have differential roles for eIF3 in the developmental regulation of translation been experimentally grounded. Furthermore, the roles of individual eIF3 subunits are not clear, and indeed some, such as the “e” subunit may have roles independent of translation initiation. The original three goals of the proposal were technically hampered by a finding that became evident during the course of the research – Any attempt to make transgenic plants that expressed eIF3e wt or eIF3e variants resulted in seedling lethality or seed inviability. That is, it was impossible to regenerate any transgenic plants that expressed eIF3e. We did manage to generate plants that expressed an inducible form of eIF3e. This also eventually led to lethality, but was very useful in elucidating the 4th goal of the research (Yahalom et al., 2008), where we showed, for the first time in any organism, that eIF3e has a repressory role in translation. In attempt to solve the expression problems, we also tried expression from the native promoter, and as such analyzed this promoter in transgenic plants (Epel, 2008). As such, several additional avenues were pursued. 1) We investigated protein-protein interactions of eIF3e (Paz-Aviram et al., 2008). 2) The results from goal #4 led to a novel hypothesis that the interaction of eIF3e and the CSN meets at the control of protein degradation of nascent proteins. In other words, that the block in translation seen in csn and eIF3e-overexpressing plants (Yahalom et al., 2008) leads to proteasome stress. Indeed we showed that both over expression of eIF3e and the csn mutants lead to the unfolded protein response. 3) We further investigated the role of an additional eIF3 subunit, eIF3h, in transalational regulation in the apical meristem (Zhou et al., 2009). Epel, A. (2008). Characterization of eIF3e in the model plant Arabidopsis thaliana. In Plant Sciences (Tel Aviv, Tel Aviv University). Paz-Aviram, T., Yahalom, A., and Chamovitz, D.A. (2008). Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome. Plant Signaling and Behaviour 3, 409-411. Yahalom, A., Kim, T.H., Roy, B., Singer, R., von Arnim, A.G., and Chamovitz, D.A. (2008). Arabidopsis eIF3e is regulated by the COP9 signalosome and has an impact on development and protein translation. Plant J 53, 300-311. Zhou, F., Dunlap, J.R., and von Arnim, A.G. The translation initiation factor subunit eIF3h is .1 involved in Arabidopsis shoot apical meristem maintenance and auxin response. (submitted to Development).
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Eshel, Amram, Jonathan P. Lynch, and Kathleen M. Brown. Physiological Regulation of Root System Architecture: The Role of Ethylene and Phosphorus. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7585195.bard.
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Specific Objectives and Related Results: 1) Determine the effect of phosphorus availability on ethylene production by roots. Test the hypothesis that phosphorus availability regulates ethylene production Clear differences were found between the two plants that were studied. In beans ethylene production is affected by P nutrition, tissue type, and stage of development. There are genotypic differences in the rate of ethylene production by various root types and in the differential in ethylene production when P treatments are compared. The acceleration in ethylene production with P deficiency increases with time. These findings support the hypothesis that ethylene production may be enhanced by phosphorus deficiency, and that the degree of enhancement varies with genotype. In tomatoes the low-P level did not enhance significantly ethylene production by the roots. Wildtype cultivars and ethylene insensitive mutants behaved similarly in that respect. 2) Characterize the effects of phosphorus availability and ethylene on the architecture of whole root systems. Test the hypothesis that both ethylene and low phosphorus availability modify root architecture. In common bean, the basal roots give rise to a major fraction of the whole root system. Unlike other laterals these roots respond to gravitropic stimulation. Their growth angle determines the proportion of the root length in the shallow layers of the soil. A correlation between ethylene production and basal root angle was found in shallow rooted but not deep-rooted genotypes, indicating that acceleration of ethylene synthesis may account for the change in basal root angle in genotypes demonstrating a plastic response to P availability. Short-time gravitropic response of the tap roots of young bean seedlings was not affected by P level in the nutrient solution. Low phosphorus specifically increases root hair length and root hair density in Arabidopsis. We tested 7 different mutants in ethylene perception and response and in each case, the response to low P was lower than that of the wild-type. The extent of reduction in P response varied among the mutants, but every mutant retained some responsiveness to changes in P concentration. The increase in root hair density was due to the increase in the number of trichoblast cell files under low P and was not mediated by ethylene. Low P did not increase the number of root hairs forming from atrichoblasts. This is in contrast to ethylene treatment, which increased the number of root hairs partly by causing root hairs to form on atrichoblasts. 3) Assess the adaptive value of root architectural plasticity in response to phosphorus availability. A simulation study indicated that genetic variation for root architecture in common bean may be related to adaptation to diverse competitive environments. The fractal dimension of tomato root system was directly correlated with P level.
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Livensperger, Carolyn. Vegetation and Soil Trends, Black Canyon of the Gunnison National Park and Curecanti National Recreation Area, 2011–2022. National Park Service, 2025. https://doi.org/10.36967/2309325.
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In 2011, the Northern Colorado Plateau Network (NCPN) began long-term monitoring of vegetation and soils in upland communities at Black Canyon of the Gunnison National Park (BLCA) and Curecanti National Recreation Area (CURE). Managers wanted to understand conditions in sagebrush shrublands, aspen forests, pinyon-juniper woodlands, and Gambel oak shrublands. These four vegetation types are associated with a range of management concerns. These include suitable habitat conditions for the threatened Gunnison sage-grouse, planning for livestock grazing management, responses to disturbances (e.g., fire, plant diseases, and insect pests), wild ungulate usage and community responses to different climate conditions. The primary goals of monitoring are to track the status of these vegetation types and identify trends that describe changes over time. Status and trend of vegetation and soil indicators were analyzed for the 2011 – 2022 monitoring period. Key findings from this analysis are: 1) In both grazed and ungrazed sagebrush shrublands, total grass cover was stable. However, in grazed sagebrush, native perennial grasses decreased over time while non-native perennial grasses increased. 2) Most elements of Gunnison sage-grouse structural habitat guidelines were within recommended ranges in both grazed and ungrazed sagebrush shrublands; however, forb cover was lower than recommended. 3) Native perennial grass cover, primarily cool-season grasses, decreased in three of four vegetation types. Aspen forests were the only vegetation type with stable grass cover. 4) Pinyon-juniper woodlands maintained stable density and cover of overstory trees, saplings, and seedlings. Non-native plant cover was low and non-native plant frequency decreased. However, native perennial grass cover also decreased. 5) Aspen forests are in decline. Overstory trees and saplings experienced mortality and decreased canopy closure. Non-native plants, primarily perennial species, had high but stable cover. 6) Gambel oak shrublands show resistance to change. Gambel oak and other shrub cover and density remained stable over time but native perennial grasses decreased. 7) Actual evapotranspiration and water deficit predicted vegetation and soil response well. Water deficit or actual evapotranspiration in June, the month with lowest precipitation, was associated with declines in native perennial grasses in sagebrush shrublands, Gambel oak shrublands, and pinyon-juniper woodlands. Future changes in these water balance variables will influence conditions in all vegetation types.