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Journal articles on the topic 'Seedling storage'
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1
Saha, P., MSU Bhuiya, B. Karmakar, et al. "Effect of Age and Storage Duration of Seedling on Growth and Yield of Wet Season Rice." Bangladesh Agronomy Journal 20, no. 1 (2017): 45–56. http://dx.doi.org/10.3329/baj.v20i1.34882.
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The experiment was conducted at the Agronomy research field of Bangladesh Agricultural University, Mymensingh from July to December 2013 to find out the effect of seedling age and storage duration on growth and yield of wet season rice. The experiment comprised of four ages of seedling (25, 30, 35 and 40-d-old) and four storage durations (0, 1, 2 and 3-days) of uprooted seedlings. Rice var. BRRI dhan52 was used as a test variety. The experiment was laid out in a split-plot design with 3 replications placing seedling age in the main plot and storage duration in the sub- plot. The effect of age and storage -duration of seedling, and their interaction were significant on growth parameters (shoot height, root length, number of tillers hill-1, leaf area index and crop growth rate), grain yield and straw yield. The growth parameters and yield exhibited a trend of decrease with the increase in seedling age and storage duration. All the growth parameters and yield showed highest value in the younger seedlings of 25-d-old with 0-day storage duration of uprooted seedlings while the lowest in the older seedlings of 40-d-old with 3-days of storage duration. The var.BRRI dhan52 produced the highest LAI (8.23), CGR (7.33 mg day-1 hill-1) and biomass (26.87 g) at 60, 45 and 60 days after transplanting. Grain yield reduced by 13, 19 and 37% of 30, 35 and 40-d-old seedlings, respectively compared to 25-d-old seedlings. In case of seedling storage, grain yield reduced by 5, 14 and 31% of 1, 2 and 3-d storage, respectively compared to 0-d storage. For optimum growth and yield of wet season rice, 25-d-old seedlings may be stored up to 1-day after uprooting taking into account the possible delay in transporting seedlings to the flood affected area from the nearby districts.Bangladesh Agron. J. 2017, 20(1): 45-56
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Goswami, Ajay Puri, Karuna Vishunavat, Chander Mohan, and Sanjeev Ravi. "Effect of seed coating, storage periods and storage containers on soybean (Glycine max (L.) Merrill) seed quality under ambient conditions." Journal of Applied and Natural Science 9, no. 1 (2017): 598–602. http://dx.doi.org/10.31018/jans.v9i1.1237.
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Soybean seed is classified as “poor storer” as it loses viability under warm and humid conditions and is invaded by storage fungi. The seed of soybean variety PS 1347, obtained from Breeder Seed Production Centre, G. B. Pant University of Agriculture and Technology, Pantnagar was used for storage study. Root length (cm), shoot length (cm), seedling fresh weight (g) and seedling dry weight (g) decreases with the increase in seed storage periods. Out of all chemical treatments, Flowable thiram @ 2.4 ml/kg (T2) and Polymer + vitavax 200 @ 2g/kg seed (T5) were found effective to maintain Seedling root length, shoot length, seedling fresh weight and seedling dry weight by producing vigorous seedlings. Seeds stored in cloth bags exhibited higher seed infection than in seed stored in polythene bags, irrespective of seed treatment and period of storage. Treatment T4 (Vitavax 200 @ 2 g/kg seed)and T5(Polymer + vitavax 200 @ 2 g/kg seed) exhibited minimum percent seed infection of Aspergillus flavus. Cloth bag was not found safe for longer storage of soybean seeds under ambient storage at Pantnagar.
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Akter, N., MM Haque, MR Islam, and KM Alam. "Seed Quality of Stored Soybean (Glycine max L.) as Influenced by Storage Containers and Storage Periods." Agriculturists 12, no. 1 (2014): 85–95. http://dx.doi.org/10.3329/agric.v12i1.19585.
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An experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during April 2013 to June 2013 to study the effect of three storage containers (tin container, polythene bag and cloth bag); and five storage periods (0, 15, 30, 45, and 60 days) on the seed quality of soybean. The experiment was laid out in Completely Randomized Design (CRD) with four replications. The soybean seeds stored in tin container showed maximum germination capacity with high germination index, highest seedling growth, seedling dry weight per plant and vigour index. The seeds stored in cloth bag had the lowest seed quality during the testing period. The highest 1000-seed weight, moisture content, electrical conductivity, abnormal seedlings and number of dead seeds were recorded in cloth bag; where the equivalent lowest values were recorded in tin container. The moisture content, 1000-seed weight, electrical conductivity, number of abnormal seedlings and dead seeds were increased with the longer period of storage. In addition, germination index, seedling growth, dry weight per plant of soybean seedling, and vigor index decreased with the increase in storage period. Among the three containers, tin container was the best and the cloth bag was the worst storage container for soybean seed storage for long period. DOI: http://dx.doi.org/10.3329/agric.v12i1.19585 The Agriculturists 2014; 12(1) 85-95
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Alvarenga, Renata Oliveira, and Julio Marcos-Filho. "Vigor evaluation of stored cotton seeds, including the Seed Vigor Imaging System (SVIS®)." Journal of Seed Science 36, no. 2 (2014): 222–30. http://dx.doi.org/10.1590/2317-1545v32n2944.
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This study aimed at evaluating efficiency of different tests to assess cotton seed vigor during storage, including the SVIS® software. Thereby, five cotton seed lots (cv. BRS 293) were stored under controlled environmental conditions of low temperature and relative humidity (10 ºC; 30% RH), and under non-controlled laboratory environmental conditions, for eight months. Evaluations of germination, first germination count, cool germination, accelerated aging test traditional and with saturated salt, field seedling emergence and seedling imaging automated analysis - SVIS® were performed before and after every two storage months. It was concluded that the cool germination, accelerated aging (traditional and with saturated salt), field seedling emergence and seedlings length (SVIS®) are adequate parameters for assessing vigor of cotton seeds during storage, and that both accelerated aging and SVIS® (vigor index and seedlings length) present enough sensitivity to identify changes on seed vigor during storage.
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Marler, Thomas E., April Cascasan, and John H. Lawrence. "Threatened Native Trees in Guam: Short-term Seed Storage and Shade Conditions Influence Emergence and Growth of Seedlings." HortScience 50, no. 7 (2015): 1049–54. http://dx.doi.org/10.21273/hortsci.50.7.1049.
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Seedling emergence and growth traits of three rare and threatened tree species in the Mariana Islands were studied within a range of incident light levels and up to 9 months of seed storage. Seedling emergence percentage and velocity were maximized in moderate shade for Elaeocarpus joga Merr., deep shade for Serianthes nelsonii Merr., and full sunlight for Tabernaemontana rotensis (Kaneh.) P.T. Li. Seedling height was increased by shade for E. joga and S. nelsonii. Height of T. rotensis seedlings was not influenced by incident light from 25% to 100%. Nine months of seed storage at ambient temperature did not influence emergence percentage of E. joga or S. nelsonii seeds. In contrast, seeds of T. rotensis began declining in seedling emergence percentage between 2 and 3 months of storage, and seedling emergence was nil by 4 months. This study represents the first experimental approach to determining the influences of light and storage on seed and seedling behavior for any rare and threatened taxa from the Mariana Islands. Our findings that revealed highly contrasting responses among the species provide a valuable start to building the knowledge base needed to respond to formal recovery or conservation plans by defining horticultural protocols for managing a conservation nursery.
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McCreary, Douglas D., and Joe B. Zaerr. "Root respiration has limited value for assessing Douglas-fir seedling quality." Canadian Journal of Forest Research 17, no. 9 (1987): 1144–47. http://dx.doi.org/10.1139/x87-175.
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Root damage is a principal cause of poor seedling quality. Currently, the most widely used seedling-quality test measures a seedling's ability to initiate and elongate roots in a favorable environment; however, this test requires a lengthy response period and tedious, time-consuming measurements. Alternatively, the respiration rate of roots could be measured much more quickly and easily. Results of this study, which examined whether root respiration could successfully predict the survival and growth of potted Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings in a growth room, indicated that respiration may help assess root damage caused by desiccation but not that caused by freezing, hot storage, or rapid heating.
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Hallgren, S. W., and C. G. Tauer. "Root Growth Potential, First-Year Survival, and Growth of Shortleaf Pine Seedlings Show Effects of Life Date, Storage, and Family." Southern Journal of Applied Forestry 13, no. 4 (1989): 163–69. http://dx.doi.org/10.1093/sjaf/13.4.163.
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Abstract Root growth potential (RGP), and first-year field survival and growth of shortleaf pine (Pinus echinata Mill.) seedlings were significantly affected by lift date, storage, and family. Seedlings lifted in December and January showed highest RGP, survival, and growth. Storage for 28 daysalways reduced survival and growth, and the effect was greatest for late-lifted seedlings. However, for late planting, the difference in performance between freshly lifted seedlings and earlier lifted and stored seedlings may be small. Large family differences existed for seasonal changesin RGP and survival, suggesting separate management by family may be beneficial. The presence of a terminal bud and secondary needles showed little value in predicting seedling performance, whereas RGP and number of primary lateral roots were strongly correlated with seedling survival. South.J. Appl. For. 13(4):163-169.
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Egnell, Gustaf, and Göran Örlander. "Using infrared thermography to assess viability of Pinussylvestris and Piceaabies seedlings before planting." Canadian Journal of Forest Research 23, no. 9 (1993): 1737–43. http://dx.doi.org/10.1139/x93-219.
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One-year-old Scots pine (Pinussylvestris L.) and 2-year-old Norway spruce (Piceaabies (L.) Karst.) seedlings were lifted and stored under an array of conditions to test infrared thermography as a means of determining seedling viability. After winter storage, temperature was measured on each seedling with an infrared thermovision scanner (3–5 μm) in an environment favoring transpiration (vapor pressure deficit of the ambient air 1.6–2.9 kPa, photosynthetic photon flux density 1500 μmol•m−2•s−1). Thereafter the seedlings were planted in the field. Visual signs of damage were assessed and annual height increments were measured after one and two growing seasons. Significant positive correlations were found between seedling temperature and degree of damage. The warmest seedlings had a lower survival rate as a group when compared with the remaining seedlings. There were significant negative correlations between seedling temperature and annual height increment in the first growing season.
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Prato, Andrés I., L. Felipe Daibes, Miguel A. Pabón, Alvaro A. Castaño, Carolina Santos-Heredia, and Claudimar S. Fior. "Seedling Emergence of the Tree Legume Clathrotropis brunnea Amshoff, Endemic from a Colombian Tropical Rainforest." Forest Science 67, no. 4 (2021): 457–67. http://dx.doi.org/10.1093/forsci/fxab013.
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Abstract Seedling emergence is the main propagation method of forest species. Thus, we aimed to evaluate emergence aspects of the tree legume Clathrotropis brunnea Amshoff (sapán or blackheart sapán), a Fabaceae of high economic value endemic to the Colombian rainforest. We characterized the biometry of legumes and seeds and assessed seedling emergence in three experiments: (1) effect of fruit color and substrate, (2) seed conservation in storage, and (3) presoaking and seed position. Our results showed that seeds of green-yellow fruits have greater length and weight and a water content of up to 53%. Seedling emergence did not differ between green-yellow and dark-brown fruits but did differ with substrate type; nearly 80% of seedlings emerged in the sand substrate (S1), but only 62% emerged in the mixed substrate (S3). Fresh sapán seeds are nondormant, showing a recalcitrant behavior in which seedling emergence decreased after storage in all tested conditions. Both seed-sowing positions allowed an emergence of >80% with a small benefit of the hilum downward, regardless of presoaking treatments. However, these differences did not affect seedling height or biomass after emergence. Our results provide basic knowledge on production of tropical seedlings, seeking species conservation, and use in restoration projects.
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Nwauzoma, A. B., and K. Moses. "Factors Affecting Seedling Emergence and Dry Matter Characteristics in Musa balbisiana Colla." ISRN Botany 2013 (August 13, 2013): 1–5. http://dx.doi.org/10.1155/2013/582581.
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The effects of storage duration (0, 2, 4, 6, 8, and 10 days), sterilization with sodium hypochlorite (0, 5, 10, 15, and 20%), and weaning media on seedling characteristics and dry matter content in Musa balbisiana seedlings were studied. The experiment was factorial in a completely randomized design with five replicates. The result indicates that increase in NaOCl concentration and number of days in storage significantly (P=0.5) increased the period of seedling emergence. Also, soaking in NaOCl for 20 min had significant effect on average seedling emergence at 15 and 20% concentrations, compared to 10 min soaking at the same concentrations. The combined effects of storage duration and sterilization resulted in a decrease in the duration of seedling emergence. Seeds previously sterilized with either water or NaOCl had no significant effect on seedling growth, leaf and corm dry weight, but affected almost all the dry matter traits. A mixture of poultry manure, top soil, and river sand as weaning media gave better seedling growth and increased dry matter characteristics. We conclude that M. balbisiana seeds require after-ripening treatment to enhance germination, sterilizing seeds with 5% NaOCl for 10 min and air-drying under ambient condition for 2–6 days were found most appropriate, and a mixture of poultry manure, top soil, and river sand is recommended as weaning medium for growth and dry matter composition in M. balbisiana seeds.
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Harper, George J., and Edith L. Camm. "Effects of frozen storage duration and soil temperature on the stomatal conductance and net photosynthesis of Piceaglauca seedlings." Canadian Journal of Forest Research 23, no. 12 (1993): 2459–66. http://dx.doi.org/10.1139/x93-305.
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Nursery grown seedlings of Piceaglauca (Moench) Voss were stored frozen in the dark from approximately 10–31 weeks, thawed and grown for 28 days in a growth chamber at three soil temperatures (3, 7, and 11 °C). During the growing period gas exchange measurements were made every three days. Seedling net photosynthesis (pn) and stomatal conductance (gs) showed significant interactions between soil temperature and storage duration treatments. Soil temperature did not affect seedling gs or pn, though the degree and extent of storage duration effects were dependent on soil temperature. Recovery of gs occurred over a 4–7 day period from low levels after planting. Seedlings stored longer than 22 weeks showed lower rates of pn, than those stored for shorter durations. The lower pn in long-stored seedlings did not result from stomatal limitations to carbon fixation, as gs increased in seedlings stored >22 weeks.
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ADEBISI, Moruf Ayodele, Tolulope Olaitan KEHINDE, Tunmise Elizabeth OLADIPO, and Isiaq Omoniyi LAWAL. "Longevity and vigour of pigeon pea (Cajanus cajan (L.) Millsp.) seed stored under humid tropical ambient conditions." Acta agriculturae Slovenica 114, no. 2 (2019): 191. http://dx.doi.org/10.14720/aas.2019.114.2.5.
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Seeds of 20 pigeon pea (<em>Cajanus cajan </em>(L.) Millsp.) genotypes were evaluated for seed longevity and vigour under humid ambient conditions. Cleaned seeds of each genotype were packed into a polyethylene bag, the packaged lot was electrically sealed and thereafter placed in a seed store under ambient conditions (30 ºC, RH 75 %). Seed samples were withdrawn at 0, 60, 120, 180 and 240 days after storage for seed quality parameters evaluation. The experiment consisted of two factors which were genotype and storage duration and was a laid out in a completely randomized design with three replications. Data were collected on rate of seed germination, seed viability, seedling length, seedlings fresh mass, seedlings dry mass and seedling vigour index. Data collected were subjected to analysis of variance and significant treatment means were separated using Tukey’s HSD test at 5 % probability level. PROBIT modelling was also used to predict the seed longevity of stored pigeon pea. Significant differences were observed in all seed quality attributes evaluated among the 20 pigeon pea genotypes and storage time except seedling fresh mass. Seed quality attributes decreased significantly with increasing storage periods. Genotypes NSWCC-18A, NSWCC-24, NSWC-34 and NSWCC-29A were identified to be superior for most of the seed quality attributes evaluated. PROBIT modelling result revealed that genotype NSWCC-29b had the highest storage life (16.28 months) and the highest storage potentials in terms of seed viability and other seed quality attributes of all seed lots.
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Bargali, Kiran, and S. S. Bargali. "Growth Versus Storage in Quercus leucotrichophora and Pinus roxburghii Seedlings in Response to Changes in Nutrient and Water Availability." Current World Environment 10, no. 2 (2015): 494–99. http://dx.doi.org/10.12944/cwe.10.2.14.
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In this paper, growth and storage of carbon and nitrogen in seedlings of banj oak (Quercus leucotrichophora A. Camus) and chir pine (Pinus roxburghii Sarg.) have been compared across different levels of nutrient and water availabilities. Four nutrient (144, 264, 384 and 504 mg of NPK fertilizer per kg soil) and three watering (21 days,14 days and 7 days interval) treatments were applied to seedlings. At low watering levels, seedling dry mass of both the species decreased towards higher nutrient level. However, at high watering level, dry mass increased with increasing water availability. When water availability was increased in a constant nutrient environment, dry mass of seedling increased with increase in water availability. Both the species showed a similar pattern of storing nitrogen instead of increasing biomass particularly at low watering levels. However, at each nutrient level, growth and storage increased with increasing moisture availability. As compared to Q. leucotrichophora, seedlings of P. roxburghii favoured growth over storage (according to its more competitive strategy), although this species accumulated more carbon and nitrogen towards the higher nutrient level.
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Ritchie, Gary A., John R. Roden, and Nick Kleyn. "Physiological quality of lodgepole pine and interior spruce seedlings: effects of lift date and duration of freezer storage." Canadian Journal of Forest Research 15, no. 4 (1985): 636–45. http://dx.doi.org/10.1139/x85-104.
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Owing to frozen ground, nurseries in interior Oregon, Washington, and British Columbia are often unable to lift bare-root seedlings during midwinter when they are in peak physiological condition. Therefore, seedlings are normally lifted in the fall and planted either immediately or after overwinter storage, or lifted in the spring and planted either immediately or after brief storage. The objective of this study was to ascertain which of the above strategies produced planting stock with the highest physiological quality. The species evaluated were lodgepole pine (Pinuscontorta Dougl.) and interior spruce (Piceaglaucaengelmannii complex). Seedlings were grown in a Weyerhaeuser Canada nursery near Armstrong, B.C., and lifted on nine dates spanning early October 1982 to late March 1983. Seedling quality was evaluated at each lift date and following 2 and 6 months in freezer (−1 °C) storage. Evaluations consisted of measurements of dormancy release index, root growth potential (RGP), stress resistance (SR), and frost hardiness (FH). The main effects of lift date and storage duration were generally highly significant on all seedling-quality attributes. Lift date × storage duration interactions were also highly significant in most cases. Dormancy weakened throughout winter in the nursery, with accumulated chilling. Storage slowed the release of dormancy for most lift dates. RGP was very high in fall-lifted seedlings, but declined rapidly after storage. RGP again increased in late winter, but was not appreciably affected by storage except for March-lifted seedlings. SR was relatively high in fall-lifted seedlings and very low in spring-lifted seedlings. SR was also reduced by storage, especially after 6 months. However, SR did not fall as rapidly in stored seedlings as it did in seedlings left in the nursery bed. FH was −30 °C in early October; during midwinter FH was beyond the limit of measurement (−40 °C). Seedlings of both species appeared to deharden in storage, but at a relatively slow rate. The best operational strategy seems to be fall lifting, beginning November 1, with overwinter freezer storage. Spring-lifted seedlings tended to exhibit low RGP, low SR, low FH, and poor storability.
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Mezzalira, Itamara, Caroline Jácome Costa, Eduardo Alano Vieira, et al. "Pre-germination treatments and storage of cassava seeds and their correlation with emergence of seedlings." Journal of Seed Science 35, no. 1 (2013): 113–18. http://dx.doi.org/10.1590/s2317-15372013000100016.
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Despite propagation of the cassava crop be done by cuttings for commercial purposes, the majority of accesses keeps active the sexual propagation system; what is essential to genetic breeding programs. Cassava seeds, however, have low and uneven germination rates. Considering such event, the objective of this study was to evaluate the efficiency of the storage, and different pre-germination treatments, on cassava seedling emergence. For this, an experiment was carried out with seeds obtained by open pollination between plants of four sweet cassava cultivars, with 10 pre-germination treatments; in addition to a control treatment. The viability of seeds subjected to different treatments was assessed by tetrazolium test; and the assessment of seedling emergence was performed by daily counts of the number of emerged seedlings. Data obtained were expressed in: emergence percentage; emergence speed index; and mean time of seedling emergence. It was concluded that storage, at 4 °C, during one year, increases seed emergence percentage and favors cassava seedling emergence speed; and that the dry heat (60 ºC), during seven and 14 days, also favors their emergence speed .
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da Rosa, S. D. V. F., A. M. Carvalho, M. B. McDonald, E. R. V. von Pinho, A,P Silva, and A. D. Veiga. "The effect of storage conditions on coffee seed and seedling quality." Seed Science and Technology 39, no. 1 (2011): 151–64. http://dx.doi.org/10.15258/sst.2011.39.1.13.
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Kubota, C., S. Seiyama, K. Sakami, and T. Kozai. "Transplant Quality as Affected by Temperature, Light Intensity, and Photoperiod during Storage." HortScience 32, no. 3 (1997): 481C—481. http://dx.doi.org/10.21273/hortsci.32.3.481c.
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Storage techniques to hold the seedlings for several weeks prior to shipping/transplanting have been required for the successful management in plug seedling production. During storage, it is required to suppress growth and development of the seedlings as well as to preserve their transplant quality. Illumination during storage has been shown to be important for storage of high-quality transplants. In the present experiments, eggplant (Solanum melongena L.) plug seedlings, which were ready for transplanting after 3 weeks of growth under 28/20C photo-/dark period temperature, 330 μmol·m–2·s–1 photosynthetic photon flux (PPF), and 16-hr photoperiod per day, were stored for 3 to 4 weeks under combinations of different temperatures, PPF, and photoperiods. Storage air temperature affected elongation of the seedlings during 3 weeks of storage. Continuous illumination at a PPF close to the light compensation point maintained dry weight of the seedlings unchanged during storage and kept the high percent survival after storage. Storage in darkness reduced the dry weight during storage and, thus, the percent survival after storage. PPF and photoperiod were shown to be important factors in the preservation of transplant quality and suppression of growth of the seedlings during storage.
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Li, Juanqi, and Yang Li. "Suitable illumination intensity is essential for preserving the quality of cucumber (Cucumis sativus L.) seedlings during storage." PLOS ONE 16, no. 3 (2021): e0247882. http://dx.doi.org/10.1371/journal.pone.0247882.
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Continuous darkness decreases seedling quality during storage, whereas appropriate light quality and intensity can overcome these negative effects. In this study, we determined the light intensity, storage time (ST), and storage temperature suitable for cucumber (Cucumis sativa L.) seedlings. We stored cucumber seedlings under four different photosynthetic photon flux densities (PPFDs; 0, 15, 30, and 45 μmol·m-2·s-1) at 12°C, and examined how the morphological, physiological, and photosynthetic changes in seedlings during storage affected their ability to recover after transplanting. Our results indicated that at least 15 μmol·m-2·s-1 PPFD was needed for cucumber seedlings stored in the dark for 2 or 4 d, and at least 30 μmol·m-2·s-1 PPFD was needed when the ST was extended to 6 d. Overall, our results showed that cucumber seedlings require light-emitting diode (LED) illumination during storage to maintain their quality and recovery ability.
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Puttonen, Pasi. "Abscisic acid concentration in Douglas-fir needles in relation to lifting date, cold storage, and postplanting vigor of seedlings." Canadian Journal of Forest Research 17, no. 5 (1987): 383–87. http://dx.doi.org/10.1139/x87-066.
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Spring-lifted seedlings were grown in pots in the field and, after a natural fall photoperiod, exposed to three 25-day cold (+4 °C) storage treatments and two lifting times, mid-November and mid-January. The storage treatments were light storage in pots, dark storage in pots, and bareroot storage in polyethylene bags in the dark. In a second experiment, an extended fall photoperiod treatment was applied to seedlings that were then stored in pots and subjected to the same light and dark treatments above. In both experiments, needle samples were taken four times during and after the treatments for abscisic acid assay. Abscisic acid concentrations were determined using gas liquid chromatography after purification with high performance liquid chromatography. Lifting times and storage treatments did not result in statistically significant differences in abscisic acid concentrations. However, there were treatment differences in characteristics of postplanting performance. Mid-November lifting resulted in reduced survival and a greater number of days to bud flush compared with the mid-January lifting results. The extended fall photoperiod material produced similar results to the natural fall photoperiod material. The failure to detect a relationship between needle abscisic acid concentration and seedling vigor may have been due to a transitory role of abscisic acid in the storage conditions studied. The quantification method for abscisic acid is insensitive and laborious for practical seedling testing.
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Anteneh, Melkam, Abebe Atilaw, and Taye Kufa. "Investigation of Coffee Seed Physical Purity, Seed Health and Effect of Storage Time on Viability." Malaysian Journal of Medical and Biological Research 1, no. 2 (2014): 85–96. http://dx.doi.org/10.18034/mjmbr.v1i2.380.
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High seed quality is essential for optimum stand establishment in Coffee. As a result, it is necessary to have seed physical, germination percent, physiological and health tests that permit rapid, objective and accurate evaluation of seed quality. This study evaluated the effect of storage time on physical, physiological, germination percent and health quality of seed lots of five coffee varieties obtained from research and commercial company. This test is conducted under ideal laboratory conditions and in the nursery site. After sample collected pure, pea-beery, cracked and shriveled seeds were measured before determining standard germination and vigor. The highest pea berry was recorded at JARC on the variety 75227(18.63), and the lowest was at LCP on the variety 74165 (8.81). In parchment coffee seed, the percentage of physical defects during seed processing affects germination and seedling viability. The standard germination test in the moisten-soft paper continues to be the most common measure of seed quality in coffee. In addition, this test requires more than two weeks before a determination of seed germination was possible. Ideally, seed quality tests efficiently differentiate between poor and good seed lots in a short period. There was high germination percent in the first planting time were recorded after one month storage than other two consecutive storage time. Normal germinated seedling reduced with in increases seed storage time and the incidence of seed/ soil -born pathogenic fungi. The germination test of seeds from laboratory under petri-dish with moist soft paper and at the nursery site also had low vigour and did not produce suitable seedlings for planting evaluated after three month storage. If after one month storage time of coffee seeds germinated more than older seeds (as our study indicates), then seedlings derived from younger may have a competitive advantage over seedlings derived from older one. In the present study, pre-emergence seedling mortality (Rotten) was greater in third month storage than in first month old seeds. This mortality partially accounted for the lower germination percentage in three month old seeds because only seeds that emerged above the soil surface were considered to have germinated is an indication of reduced vigor. In my study, in coffee seeds, seedlings from relatively low stored seeds were generally better able to withstand environmental stress than those from old seeds. Coffee seed sample from two sources stored safely to optimize coffee seedling production at the appropriate time and season with ideal climatic conditions for planting in the field.
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Wilson, Sandra B., Keiko Iwabuchi, Nihal C. Rajapakse, and Roy E. Young. "Responses of Broccoli Seedlings to Light Quality during Low-temperature Storage in Vitro: I. Morphology and Survival." HortScience 33, no. 7 (1998): 1253–57. http://dx.doi.org/10.21273/hortsci.33.7.1253.
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Broccoli (Brassica oleracea L. Botrytis group `Green Duke') seeds were cultured in vitro photoautotrophically (without sugar in the medium) or photomixotrophically (with sugar in the medium) for 3 weeks at 23 °C and 150 μmol·m-2·s-1 photosynthetic photon flux (PPF). Vessels were then stored at 5 °C under 1.6, 4.1, or 8.6 μmol·m-2·s-1 of white (400-800 nm), red (600-700 nm), or blue (400-500 nm) light. Concentrations of CO2 inside the vessels were monitored until equilibrium was reached. Light compensation point was reached at 3.5 μmol·m-2·s-1 for photoautotrophic seedlings and at 6.5 μmol·m-2·s-1 for photomixotrophic seedlings. Therefore, in the long-term storage experiment, seedlings were stored for 4, 8, or 12 weeks at 5 °C in darkness or under 5 μmol·m-2·s-1 (average light compensation point) of white, red, or blue light. Illumination during storage was necessary to maintain dry mass, leaf area, and regrowth potentials of in vitro seedlings. All seedlings stored in darkness were of poor quality and died when transferred to the greenhouse. Red light during storage increased seedling dry mass and chlorophyll content and improved overall appearance, whereas blue light decreased chlorophyll content and increased stem elongation. The addition of 2% sucrose to media increased dry mass and leaf area and maintained overall seedling quality during illuminated storage. However, plantlets stored for more than 4 weeks did not survive poststorage greenhouse conditions, regardless of light treatment.
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Stanisavljevic, Rade, Dragoslav Ðjokic, Jasmina Milenkovic, et al. "Seed germination and seedling vigour of italian ryegrass, cocksfoot and timothy following harvest and storage." Ciência e Agrotecnologia 35, no. 6 (2011): 1141–48. http://dx.doi.org/10.1590/s1413-70542011000600014.
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During post-harvest maturation, different species vary in the length of dormancy breaking or germination increases. Seed dormancy and slow seedling development often limit establishment of forage grass stands. Seed germination and seedling vigour of Italian ryegrass (Lolium italicum A. Braun, Synonym Lolium multiflorum L.), cocksfoot (Dactylis glomerata L.) and timothy (Phleum pretense L.) were observed after harvest and storage. After harvest in June, seeds were stored under standard storage conditions and sampled every 30 days after harvest (DAH), up to 270 DAH, and then every 60 days up to 990 DAH. At each date, seeds were tested for final germination percentage and for seedling vigour traits. Timothy seeds had a maximum germination (88%) and the best seedlings vigour at 90 DAH, which implies that early autumn (September-October) is the best sowing period for freshly harvested seeds of timothy. Timothy seed germination was poor from 270 DAH (73%). The best germination and vigour of Italian ryegrass and cocksfoot seedlings were between 270 and 330 DAH, which equates to spring sowing time (March-April) in the succeeding year. Cocksfoot and Italian ryegrass seeds maintained satisfactory germination levels up to 630 DAH (81%) and 810 DAH (81%), respectively. The data can serve for the determination of a proper storage duration management between harvest and sowing of the tested species under ambient conditions of south-eastern Europe.
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Stanisavljevic, Rade, Dobrivoj Poštić, Ratibor Štrbanović, et al. "Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses." Tropical Grasslands-Forrajes Tropicales 8, no. 2 (2020): 125–32. http://dx.doi.org/10.17138/tgft(8)125-132.
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Tests of seed germination, seed dormancy and seedling growth were performed on 0-, 6-, 20- and 30-months-old seed lots of Festulolium in comparison with Italian ryegrass (Lolium multiflorum) and meadow fescue (Festuca pratensis). Tests were performed on seeds harvested in 2 different years (2014 and 2015) resulting in no major difference between the years. Seed storage affected seed viability and dormancy and seedling growth in all 3 grasses. The maximum germination of Festulolium seeds was achieved 6 months after harvest (95% normal seedlings); germination decreased significantly thereafter. While maximum germination of L. multiflorum and F. pratensis seeds was also achieved following storage for 6 months, these germination rates (93 and 90%, respectively) were retained until at least 20 months in storage. After storage for 30 months, seed germination of Festulolium, L. multiflorum and F. pratensis had declined to 72, 79 and 83%, respectively. High germination in all species was associated with higher rates of seedling growth. In an artificial seed ageing test, a temperature of 41 °C (during 48 and 72 hours) was found to effectively rank seed lots for germination performance in all 3 grasses. This test seems to have application for use in the seed trade to identify seed lots which could deteriorate more rapidly in storage. Further studies are needed to verify this hypothesis.
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Tambunsaribu, Dian Wirawan, Syaiful Anwar, and Dwi Retno Lukiwati. "Viabilitas benih dan pertumbuhan bibit kakao (Theobroma cacao L) pada beberapa jenis media simpan dan tingkat kelembaban." Journal of Agro Complex 1, no. 3 (2017): 135. http://dx.doi.org/10.14710/joac.1.3.135-142.
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The research aimed to study the effect of type and humidity level of storage media and teir interaction on cacao seed viability and seedling growth. The experiment was arranged in a completely randomized factorial design 3 x 4 with three replications. The first factor is the type of storage media (P) which is control (P0), charcoal shell (P1), and sawdust (P2). The second factor is the humidity content of storage media (K) which is control (K0), moisture content 15% (K1), moisture content 30% (K2), moisture content 45% (K3). Parameters observed were seeds moisture content, electrical conductivity (EC), seed germination, germination vigor, germination rate, height of seedling, leaf number of seedling, dry weight of seedling and hypothetic vigor index of seedling. Data were analyzed by anova and followed Duncan’s Multiple Range Test (DMRT). The treatments of charcoal shell and sawdust gave not significant effect to cacao seed viability and seedling growth, but it’s interactions gives significan effect the observed parameters. Combined use of charcoal shell storage media with humidity content 30% gives the highest result to cacao seed viability and seedling growth after storage.Keywords : seed viability, storage media, humidity level and cacao
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Hutauruk, Deswidya, Dwi Suryanto, and Erman Munir. "ASAI ISOLAT BAKTERI KITINOLITIK BACILLUS SP. BK17 PADA MEDIA PEMBAWA TANAH GAMBUT DAN KOMPOS JANJANG KELAPA SAWIT DALAM MENGHAMBAT PERTUMBUHAN JAMUR PATOGEN SCLEROTIUM ROLFSII DAN FUSARIUM OXYSPORUM PADA KECAMBAH CABAI." JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA 16, no. 1 (2016): 61. http://dx.doi.org/10.23960/j.hptt.11661-70.
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Assay of chitinolytic bacterial isolate of Bacillus sp. Bk17 in peat and palm oil bunch compost as carrier media in inhibiting Sclerotium rolfsii and Fusarium oxysporum of chilli seedlings. Sclerotium rolfsii and Fusarium oxysporum have been known as causal agents of seedling-off of chilli. Biological control has been used as an alternative control to replace chemical control. This study was aimed to determine the viability and ability of chitinolityc bacteria Bacillus sp. BK17 in carrier media of peat and palm oil bunch compost and in growing media to control seedling-off caused by S. rolfsii dan F. oxysporum of chilli. Our previous study showed that Bacillus sp. BK17 could reduce disease severity and intensity. Bacterial viability was measured as colony number grown after 90 days of storage in minimum salt medium with colloidal chitin as sole C source. Reduction of disease infection was measured as seedling number infected by S. rolfsii dan F. oxysporum. Seedling performances were measured as seedling height, leaf number and dry-weight after 30-days of growth. The result showed that bacterial cell viability was still high in both peat and palm oil bunch compost both with and without colloidal chitin addition after 90 days of storage. It was also shown that during application bacterial cell could grow. Seedling performaces i.e. seedling height, leaf number and dry-weight showed to be normal or even increase compared to those of pathogenic fungal inoculation only and (-) control.
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,, Saipulloh, Endah Retno Palupi, Eny Widajati, and Dan Nurita Toruan Mathius. "Efektivitas Bahan Pelapis Benih terhadap Penyerapan Fosfat dan Pertumbuhan Bibit Kelapa Sawit." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 45, no. 1 (2017): 86–92. http://dx.doi.org/10.24831/jai.v45i1.12236.
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One way of overcome phosphate deficiency in oil palm plantation is seed enrichment using phosphate solvent microbes. This technique is expected to accelerate phosphate availability for plant. The aim of the research was to identify the best coating materials for seed enrichment that compatible with Burkholderia sp. in order to increase phosphate absorption, seed storability, and growth of oil palm seedlings. This research consisted of two consecutive experiments. The first experiment was arranged in randomized block design, with nine treatments of different seed coating materials. The second experiment was arranged in nested design, with main factor of 11 different seed coating materials and nested factors were five storage periods. The data was collected on growth parameters of seedling height, root length, trunk height, dry weight; phosphate absorption, and DGGE analysis. The research showed there were three best seed coating materials of CMC 1.5%, CMC 2% + gypsum 1.5%, and CMC 1.5% + talc 1%. The coating material enhances seedling growth and phosphate absorption. Seed coating and enrichment maintained seedling vigor for three days in the storage. Keywords: Burkholderia sp., carboxyl methyl cellulose, phosphate solvent microbial, seed enrichment, seed storage
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Rose, Robin, Steven K. Omi, Barry Court, and Kathy Yakimchuk. "Dormancy release and growth responses of 3+0 bare-root white spruce (Piceaglauca) seedlings subjected to moisture stress before freezer storage." Canadian Journal of Forest Research 22, no. 1 (1992): 132–37. http://dx.doi.org/10.1139/x92-018.
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Greenhouse and field experiments were conducted with white spruce (Piceaglauca (Moench) Voss) seedlings from three seed sources to determine how plant moisture stress before storage and length of freezer storage (8–209 days) affect dormancy release and growth responses of outplanted seedlings. During storage, plant water potential remained lower (more negative) in seedlings subjected to high prestorage plant moisture stress than in those subjected to low prestorage plant moisture stress. However, as duration of storage increased, overall plant moisture stress declined. High prestorage plant moisture stress had little influence on dormancy except to delay terminal bud break in seedlings from the southernmost seed source by 10% and, after an 8-day storage period, to delay bud break of seedlings by 15% compared with the low moisture stress treatment. Seed source and storage period were associated with the greatest variation in growth. The central seed source produced more growth in height and biomass than the other seed sources. Better height growth of seedlings subjected to high moisture stress was attained after all but the 8-day storage period. Seedling responses were complex and not clearly related to their performance in nursery and field outplantings.
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Overton, E. C., A. S. Davis, and J. R. Pinto. "Insights into big sagebrush seedling storage practices." Native Plants Journal 14, no. 3 (2013): 225–30. http://dx.doi.org/10.3368/npj.14.3.225.
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Quettier, Anne-Laure, and Peter J. Eastmond. "Storage oil hydrolysis during early seedling growth." Plant Physiology and Biochemistry 47, no. 6 (2009): 485–90. http://dx.doi.org/10.1016/j.plaphy.2008.12.005.
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Wang, Yingfang, and Janusz J. Zwiazek. "Effects of storage temperature on physiological characteristics of fall-lifted white spruce (Picea glauca) bareroot seedlings." Canadian Journal of Forest Research 29, no. 6 (1999): 679–86. http://dx.doi.org/10.1139/x99-038.
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Fall-lifted white spruce (Picea glauca (Moench) Voss) bareroot seedlings were winter stored at -2, -4, and -6°C. The electrolyte leakage and carbohydrate content were periodically measured in roots and shoots during storage. Water relations, gas exchange, bud flushing, and root growth potentials were measured after 4 and (or) 7 months of frozen storage. Shoots and roots of seedlings stored at -6°C had higher electrolyte leakage compared with those stored at -2 and -4°C. Sugar levels increased as a result of frozen storage and were higher in seedlings stored at -4 and -6°C compared with those at -2°C. Root total nonstructural carbohydrate (TNC) contents declined with storage duration in all storage temperatures. However, seedlings stored at -4 and -6°C maintained slightly higher root TNC than those stored at -2°C. Needle TNC in seedlings stored at -4 and -6°C did not change; however, it declined in seedlings stored at -2°C. Storage temperatures of -4 and -6°C also altered seedling water relations with seedlings stored at these temperatures having lower osmotic potentials at turgor loss point and at full hydration and less elastic cell walls compared with seedlings stored at -2°C. Lower storage temperatures decreased root growth potentials and delayed bud flushing following planting.
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Silva, Isneider Luiz, Fernando Ribeiro Teles de Camargo, Raniele Tadeu Guimarães de Souza, Itamar Rosa Teixeira, and Hamilton Kikuti. "Storage of soybean seeds treated with chemicals." Semina: Ciências Agrárias 40, no. 6Supl2 (2019): 2961. http://dx.doi.org/10.5433/1679-0359.2019v40n6supl2p2961.
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To treat seed lots during the beneficiation process industrial seed treatment (TIS) has been used on seedlings. However, the actual TIS influence on the physiological quality of soybeans throughout storage is not yet known. The objective of this work was to evaluate the effects of TIS on the physiological quality of seeds of soybean cultivars during storage. Three soybean cultivars (M7110 IPRO, RR-8473RSF, M7739 IPRO) were subjected to four chemical treatment combinations (TIC-treated fungicide / insecticide): T1 - control; T2 - fungicide (Derosal Plus® - 200mL); T3 - insecticide (Cruiser ® - 500mL); T4 - a mixture of fungicide and insecticide products metalaxyl + thiabendazole + fludioxonil (MaximAdvanced - 200 mL) and thiamethoxam insecticide (Cruiser 350 FS - 500 mL). The samples were stored for a total of six months, with evaluation before storage and monthly throughout the storage period (0, 30, 60, 90, 120, 150 and 180 days). The seeds were stored in a cold chamber regulated at 12 ± 2 ºC and a relative humidity of 45± 2%. In addition to determining the water content, the following germination and vigor tests were performed: first count, seedling length, seedling dry mass and accelerated aging. A completely randomized design was used, in a 3 x 4 x 7 factorial scheme, with four replications. The data were initiallysubjected to analysis of variance, and later discriminated by the Tukey test (p < 0.05) (cultivars and chemical products) and regression (storage period). The work concludes that the use of insecticide favored the length of seedlings in cultivar RR-8473 RSF. Chemicals affected the quality and vigor of seeds in all studied cultivars. Soybean seeds can be stored with chemical treatment for commercial purposes for up to 60 days.
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Kaczperski, Mark P., Allan M. Armitage, and Pamela M. Lewis. "Performance of Plug-grown Geranium Seedlings Preconditioned with Nitrogen Fertilizer or Low-temperature Storage." HortScience 31, no. 3 (1996): 361–63. http://dx.doi.org/10.21273/hortsci.31.3.361.
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Pelargonium×hortorum L.H. Bailey `Scarlet Elite' seedlings were grown in plugs from seed to transplant size. About 14 days before attaining transplant size, seedlings were exposed to various fertility or temperature regimes (preconditioning treatments), then stored for 1 to 3 weeks at 5C. Seedlings receiving 150 mg N/liter before storage flowered sooner and required less crop time (days to flower – days in storage) than those receiving 0, 75, or 300 mg. Temperature preconditioning at 10 or 15C delayed flowering compared to preconditioning at 20C. Final plant height and dry weight were not adversely affected by varying N levels or temperature during preconditioning. Preconditioning seedlings with 300 mg N/liter resulted in seedling mortality rates up to 16% after 7 days' storage. Low temperature or fertility were not effective preconditioning treatments. Best results were attained by preconditioning seedlings with 150 mg N/liter.
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Ketring, Darold L. "Physiology of Oil Seeds. X. Seed Quality of Peanut Genotypes as Affected by Ambient Storage Temperature1." Peanut Science 19, no. 2 (1992): 72–77. http://dx.doi.org/10.3146/i0095-3679-19-2-3.
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Abstract Proper storage of peanut (Arachis hypogaea L.) pods following drying is a critical step in maintenance of seed quality for the next planting season. The objective of this study was to examine the effect of ambient storage temperature (similar to farmers stock peanuts stored in warehouses) on seed germination and seedling vigor of selected peanut genotypes. Peanuts were grown in the field for three successive seasons. Pods were harvested, dried, and shelled. Seed samples were taken for storage under ambient conditions, humidity effects were eliminated by storing the seeds in plastic freezer bags in sealed containers. Seasons were: (1) 1986, 19 months storage at ambient temperature, (2) 1987, 7 months storage at ambient temperature, and (3) 1988, seeds without storage. When comparisons were made among genotypes within seasons, exposure to Season 1 conditions resulted in the least germination and seedling growth, but a wide range of genotype diversity occurred. Germination of seeds from Seasons 2 and 3 ranged from 81 to 98%, and significant differences in seedling growth occurred among genotypes. When comparisons were made across Seasons, the data indicated a significant storage effect, which resulted in different seed quality for individual genotypes. Usually field emergence was significantly different among genotypes and was highly correlated with germination for all seasons. Generally, emergence was negatively correlated with slow seedling growth and positively correlated with rapid seedling growth. Thus, for seeds of lower vigor (Seasons 1 and 2), rapid seedling growth was particularly critical for early, uniform emergence (10 DAP) in the field. Genotypes were significantly different in extent of seed quality reduction and field emergence both within and across storage periods. Genotype diversity to ambient storage conditions suggests there is genetic potential to improve longevity of seed quality during storage and enhance stability of field emergence.
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Nasiro, Kalifa. "INFLUENCE OF SEED INITIAL MOISTURE CONTENT, STORAGE CONDITION AND TIME OF STORAGE ON SEEDLING GROWTH STAGES OF COFFEE (COFFEA ARABICA L.)." Agrobiological Records 4 (2021): 1–7. http://dx.doi.org/10.47278/journal.abr/2020.024.
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Coffee is one of the most important agricultural products in the international market and many countries are involved in its production, trade or consumption. Arabica coffee is self-pollinated, homozygous, and normally propagated by seeds. Seeds have been considered intermediate storage behavior with varying results. It is highly desirable that seeds are stored safely to optimize coffee seedling production at the appropriate time and season. The objective of this study was to evaluate the effect of storage temperature, time of storage and initial seed moisture contents on early seedling performances of coffee seeds and to determine the appropriate seed handling method. In this experiment, the influence of initial seed moisture content with four levels (12, 17, 22 & 27%) with storage two temperature (15oC & ambient), time of storage with six levels (sowing after each month and upto 6 months) and on coffee seedling growth stages were studied in a split-split-plot factorial design. The data collected were subjected to ANOVA. The storage environment with cold temperature (15oC) accelerated seedling growth stages parameters much better performances than did ambient temperature condition. All tested seedling growth stages were faster at initial time of storage. After third month seed quality drastically reduced especially under ambient storage condition. Seeds dried to 12% moisture content showed delayed performance throughout the trial period. Seeds with 27% initial moisture content took shorter days to reach at different growth stages at initial storage time but when aged took much days. Storage temperature, time of storage and initial seed moisture contents showed highly significant main and interaction effects and seeds dried to intermediate moisture level (17 and 22%), stored under cold temperature and sown at early times resulted in enhanced seedling growth. It was concluded that drying coffee seeds to 17% to 22% moisture contents and kept under storage with relatively lower temperatures (15oC) for not more than six months of storage showed better performance.
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Roberts, Dane R., Peter Toivonen, and Stephanie M. McInnis. "Discrete proteins associated with overwintering of interior spruce and Douglas-fir seedlings." Canadian Journal of Botany 69, no. 3 (1991): 437–41. http://dx.doi.org/10.1139/b91-060.
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Seasonal protein changes were followed in seedlings of interior spruce (a mixture of Picea glauca and P. englemannii) and Douglas-fir (Pseudostuga menziesii) by sodium dodecylsulfate – polyacrylimide gel electrophoresis. A 30-kDa protein in seedlings of Douglas-fir and a 30- and 27-kDa protein in interior spruce that were not detected in the late summer accumulated in seedling tissues during the fall. These proteins remained present throughout the winter but declined rapidly in seedlings during the initial flush of spring growth. There was an increase in the total protein content of interior spruce seedling tissues during the fall; however, the accumulation of the 30- and 27-kDa protein was tissue specific, since it increased in the apical bud, shoot, and root tissue but not in the leaves. By late fall these proteins represented approximately 15% of the total seedling protein. Measurements of variable chlorophyll fluorescence indicated that the accumulation of these overwintering proteins was associated with the seasonal decline in photosynthetic capacity. These results suggest that conifer seedlings may utilize these proteins as a storage reserve during overwintering. Key words: conifers, electrophoresis, overwintering, proteins, seasonal, seedlings.
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Cregg*, Bert. "Defoliating Lilac Seedlings in Field Production." HortScience 39, no. 4 (2004): 820A—820. http://dx.doi.org/10.21273/hortsci.39.4.820a.
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Lilac (Syringa vulgaris L) seedlings are commonly grown in many seedling nurseries in Michigan. Typically seedlings are lifted in the fall and stored prior to shipment or stored by the customer. A major problem in field production of lilacs is that seedlings often retain their leaves late in the fall. If the leaves are not removed prior to storage or shipment, the seedlings will mold and deteriorate. Therefore, growers must spend additional labor to remove the leaves, often by hand. The goal of this research was to evaluate chemical alternatives to defoliate lilac seedlings in field nurseries. Two on-farm research trials were conducted in 2001 and 2003 in cooperation with a seedling grower in Saugatuck, MI. In Experiment 1, Florel (1/2 and ¼ dilution) and chelated copper (0.5% and 1% solution) were sprayed by and onto lilac in the seedling bed. Florel and chelated copper effectively reduced leaf area of lilac seedlings. Less than 20% of the initial leaf area remained on the 1% copper and ½ Florel-treated seedlings. The ½ Florel and 1% chelated copper completely defoliated 67% and 40% of the seedlings, respectively, whereas only 17% on the control seedlings lost all their leaves prior to lifting. Both levels of Florel and the 1% copper treatment reduced growth of seedlings after planting. In experiment 2, we applied chelated copper treatments at varying rates (0.25% and 0.5%) and times (1 application and 2 applications) using the cooperators' spray equipment. Repeated applications of chelated copper were more effective in reducing seedling leaf area than a single application at both concentrations tested.
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Sultana, Razia, M. Salahuddin M. Chowdhury, Md Rafiqul Islam, and Khadija Akhter. "Effects of Container and Duration of Storage on the Quality of Okra (Abelmoschus esculentus) Seeds." Agriculturists 14, no. 1 (2016): 63–72. http://dx.doi.org/10.3329/agric.v14i1.29101.
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A study was conducted in the laboratory of the Department of Seed Pathology, Sher-e-Bangla Agricultural University, Bangladesh during July 2013 to June 2014 to determine the effects of different storage containers and durations of storage on the quality of okra (Abelmoschus esculentus) seeds and seedling growth. After harvest, seeds were stored in earthen pots, plastic pots, plastic bags, gunny bags with and without polyethylene lining for the periods of 4, 8, and 12 months. The seeds stored in plastic container showed the lowest moisture content (8.03%) at 4 months of storage and those stored in gunny bag showed the maximum moisture content (17.63%) at 12 months of storage. Percentage of seeds infested with fungi was minimum in plastic container, where the prevalence of Fusarium spp., Chaetomium globosum, Aspergillus flavus, Aspergillus niger, Rhizopus stolonifer and Curvularia spp. were 1.47, 8.03, 3.31, 3.31, 2.57 and 3.53%, respectively at 4 months of storage. Prevalence of fungi was maximum in seeds stored in gunny bag where the prevalence of Fusarium spp., Chaetomium globosum, Aspergillus flavus, Aspergillus niger, Rhizopus stolonifer and Curvularia spp. were 4.87, 17.63, 4.41, 5.97, 84.86 and 5.19%, respectively at 12 months of storage. In contrast, treated seeds stored in plastic container showed the maximum germination (94.74%), longest shoot (20.76 cm) and root (11.52 cm), highest seed vigor index (3087.00), highest weight of dry seedlings (55.60 g) at 4 months of storage. However, seeds stored in gunny bag showed the lowest seed germination (45.08%), shoot length (11.49 cm), root length (6.34 cm), seedling vigor index (838.10), dry weight of seedlings (33.40 g) and field emergence (37.36%) at 12 months of storage. Plastic containers appeared to be the best for storage of okra seeds.The Agriculturists 2016; 14(1) 63-72
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Barnett, James P., John C. Brissette, Albert G. Kais, and John P. Jones. "Improving Field Performance of Southern Pine Seedlings by Treating with Fungicides before Storage." Southern Journal of Applied Forestry 12, no. 4 (1988): 281–85. http://dx.doi.org/10.1093/sjaf/12.4.281.
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Abstract Field survival of longleaf, shortleaf, slash, and loblolly pine seedlings that had benomyl incorporated into the packing medium was markedly improved over that of clay-slurry controls. The more difficult to store seedlings of longleaf and shortleaf pine had greater magnitudes of response than more easily stored loblolly and slash pines. The decrease in seedling survival with storage time is related to a rapid increase in pathogenic microorganisms in cold storage. Although Benlate® ¹ 50WP is now registered for this use, the most effective fungicide dosage rates for routine use with different southern pine species must still be determined. South. J. Appl. For. 12(4):281-285.
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Miled, D. D. Ben, M. Zarrouk, and A. Chérif. "Sodium chloride effects on lipase activity in germinating rape seeds." Biochemical Society Transactions 28, no. 6 (2000): 899–902. http://dx.doi.org/10.1042/bst0280899.
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Seeds of rape (Brassica napus L.) were germinated at various NaCl concentrations up to 200 mM. Germinating levels, seedling growth, triacylglycerol mobilization and lipase activity were investigated. High salt concentrations resulted in retardation of seed germination. Seedling growth as measured by radicle length was severely reduced by NaCl doses higher than 50 mM. Moreover, the mobilization of storage oil in control rapeseed seedlings, started about 24 h after imbibition. As for germination and growth, elevated salt concentrations are found to delay triacylglycerol degradation. Experiments using triolein as substrate indicated clearly that lipase activity was inhibited by salt treatment.
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Riikonen, Johanna, and Jaana Luoranen. "An Assessment of Storability of Norway Spruce Container Seedlings in Freezer Storage as Affected by Short-Day Treatment." Forests 11, no. 6 (2020): 692. http://dx.doi.org/10.3390/f11060692.
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Determination of safe times at which to transfer seedlings to freezer storage is problematic in forest tree nurseries. The present study aimed to determine the relationship between pre-storage frost hardiness (FH) of different plant parts, dry matter content (DMC), chilling hours (the sum of hours when temperature was between −5 °C and +5 °C), and post-storage vitality, and the impact of short-day (SD) treatment on these relationships. One and a half year old control seedlings and SD-treated seedlings of Norway spruce were transferred to freezer storage (−3 °C) on five occasions during autumn. On each occasion, the FH of buds, needles, stem, and roots, as well as DMC, were determined, and chilling hours were calculated. The vitality of the freezer-stored seedlings was determined through their root growth capacity in the subsequent spring, and through the field performance of the seedlings (shoot growth and seedling damage) at the end of the following two growing seasons. Seedlings were considered to be storable when the FH of the needles was at least −25 °C, and the FH of the roots was about −10 °C in both treatments. Early storage reduced the vitality of the seedlings. SD treatment did not advance the storability of the seedlings, although it alleviated some of the negative effects of early storage by improving the FH of needles and stem, but not that of the roots. The DMC value, indicating storability, was higher for SD-treated seedlings than for control seedlings. When data from five experiments conducted in Suonenjoki were combined, it was found that the relationship between accumulation of chilling hours and needle FH was dependent on nursery treatment and assessment year, which reduces the reliability of using chilling hours in predicting the storability of Norway spruce seedlings. The predicted climate change may complicate the fall acclimation of seedlings. New, user-friendly methods for determining storability of seedlings are urgently needed.
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Maciel, C. G., G. M. S. Ribeiro, D. S. S. Lima, S. L. A. Oliveira, and A. C. Pessoa. "Storage of ipê seeds in different packages and environments." Scientific Electronic Archives 13, no. 6 (2020): 36. http://dx.doi.org/10.36560/13620201002.
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The adequate storage will reflect directly on the physical, physiological and sanitary quality of the forest seeds and consequently on the process of seedling production. In this sense, the determination of suitable conditions for the storage of the seeds using packages and environments accessible to the producer are essential. Therefore, the objective of this work was to evaluate different packages (glass, plastic bag and kraft paper) and environments (refrigerator, freezer and laboratory environment) for storing seeds of Tabebuia chrysotricha (Mart. Ex DC.) Standl. (ipe). The variables germination, normal seedlings, abnormal seedlings and dead seeds were evaluated every 30 days for six months. The storage of yellow ipê seeds can be carried out in a plastic bag in the refrigerator (up to 150 days) and in the freezer (up to 180 days) with up to 70% germination.
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Hartmann Filho, Cesar Pedro, André Luís Duarte Goneli, Tathiana Elisa Masetto, Elton Aparecido Siqueira Martins, and Guilherme Cardoso Oba. "The effect of drying temperatures and storage of seeds on the growth of soybean seedlings." Journal of Seed Science 38, no. 4 (2016): 287–95. http://dx.doi.org/10.1590/2317-1545v38n4161866.
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Abstract: Drying of seeds reduces their moisture content to levels appropriate for storage. However, care in the temperatures applied in the process is necessary to avoid damage to the seeds that are dried. The aim of this study was to evaluate the effect of different drying temperatures and storage on the growth of soybean seedlings. Harvested with a moisture content of approximately 23% (w.b.), the seeds were dried at different temperatures (40, 50, 60, 70, and 80 °C) until reaching moisture content of 12.5 ± 0.7% (w.b.), and they were subsequently stored for 180 days in an environment without climate control. A germination test and evaluations of seedling performance were carried out every 45 days, determining the full length of the seedling and hypocotyl and root lengths, along with their respective dry matter weights. The results showed that: a) the increase in the temperature of drying air affects the physiological quality of soybean seeds, and this effect is accentuated over time, especially on root length; and b) the air temperature of 40 °C can be recommended for drying of soybean seeds in association with the storage time of 180 days under storage conditions without climate control.
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Penfield, Steven, Helen M. Pinfield-Wells, and Ian A. Graham. "Storage Reserve Mobilisation and Seedling Establishment in Arabidopsis." Arabidopsis Book 4 (January 2006): e0100. http://dx.doi.org/10.1199/tab.0100.
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SATO, Fumio, Hiroshi YOSHIOKA, and Takahiro FUJIWARA. "Effects of Storage Temperature on Carbohydrate Content and Seedling Quality of Cabbage Plug Seedlings." Environment Control in Biology 37, no. 4 (1999): 249–55. http://dx.doi.org/10.2525/ecb1963.37.249.
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Lefevre, Roy E., Arthur C. Cameron, and N. Curtis Peterson. "Influence of Moisture Loss During Storage on New Growth of Conifer Seedlings." Journal of Environmental Horticulture 9, no. 2 (1991): 92–96. http://dx.doi.org/10.24266/0738-2898-9.2.92.
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Abstract The relationship between moisture loss from bare-root 2-year-old seedlings of Colorado spruce (Picea pungens glauca Engelm.) and percent survival and performance after extended storage at 0°C (32°F) was investigated. Moisture loss was examined by percent weight loss and moisture content (g H2O/g FW) during postharvest handling and storage. To generate different levels of moisture loss, seedlings were stored in various packages that differed in water permeability with a 0 and 1 day delay at 20°C (68°F) prior to 5 or 7 months of storage at 0°C (32°F). Depending on treatment, percent weight loss of seedlings varied from 0 to 60% and seedling survival ranged from 0 to 100%. When percent weight loss was below 25% all bare-root seedlings survived 5 and 7 months storage. Seedlings expressed a great degree of tolerance to desiccation stress, as demonstrated by those few which survived nearly 60% weight loss. Between 20 and 40% weight loss there was no direct relationship between moisture loss and percent survival. Bare-root Colorado Spruce seedlings grew with 100% survival following 5 and 7 months storage at 0°C (32°F) if packaged in either unsealed perforated or nonperforated polyethylene film.
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Spalholz, Hans, and Chieri Kubota. "Rootstock Affected In- and Poststorage Performance of Grafted Watermelon Seedlings at Low Temperature." HortTechnology 27, no. 1 (2017): 93–98. http://dx.doi.org/10.21273/horttech03577-16.
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Low-temperature storage is a technique to hold seedlings for a short period of time to adjust the production schedule of young seedlings. Labor-intensive grafting propagation can potentially benefit from the effective use of this technique to minimize peak labor inputs. Watermelon (Citrullus lanatus) seedlings are generally chilling sensitive and therefore difficult to store at low temperatures. However, the rootstocks used for watermelon grafting, interspecific squash (Cucurbita maxima × Cucurbita moschata) and bottle gourd (Lagenaria siceraria) are known to be chilling tolerant. To examine the influence of rootstocks on storability of watermelon seedlings, young seedlings of ‘Tri-X-313’ seedless watermelon grafted onto ‘Strong Tosa’ interspecific squash, ‘Emphasis’ bottle gourd, and ‘Tri-X-313’ watermelon as rootstock were placed for 2 or 4 weeks under 12 °C air temperature and 12 μmol·m−2·s−1 photosynthetic photon flux (PPF). Nongrafted watermelon seedlings were also treated in these same conditions. In addition, nonstored (grafted and nongrafted) seedlings were prepared for comparison. Regardless of seedling type (nongrafted or grafted with different rootstocks), all seedlings stored for 2 weeks had lower dry weight, comparable or greater number of leaves and stem length, when compared with their respective nonstored control groups after 2 weeks in the greenhouse. Seedlings stored for 4 weeks had lower number of leaves and stem length after 2 weeks in the greenhouse, except for those grafted onto the interspecific squash rootstock. Nongrafted and grafted watermelon seedlings with the same watermelon cultivar as rootstock showed significantly lower leaf net photosynthetic rates after 2 weeks in the greenhouse after the 2-week storage than those of nonstored control groups. In contrast, when grafted onto interspecific squash and bottle gourd rootstocks, seedlings showed comparable net photosynthetic rate to the control group. For all seedling types, 20% to 35% of seedlings died during 4-week storage or poststorage in the greenhouse, whereas all seedlings survived for the 2-week storage, except when grafted onto watermelon as rootstock. Therefore, chilling-tolerant rootstocks ‘Strong Tosa’ interspecific squash and ‘Emphasis’ bottle gourd improved storability of grafted ‘Tri-X-313’ watermelon seedlings but could not extend the storability beyond 2 weeks.
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Rodrigues, Gabrielly Bernardes, Osvaldo Resende, Lígia Campos de Moura Silva, and Weder Nunes Ferreira Junior. "Physiological quality of graniferous sorghum seeds during storage." Research, Society and Development 9, no. 6 (2020): e27963152. http://dx.doi.org/10.33448/rsd-v9i6.3152.
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The present study aimed to evaluate the effect of different drying temperatures and storage times on the physiological quality of graniferous sorghum seeds. Graniferous sorghum seeds were manually harvested after maturation, with moisture content of approximately 21% d.b., in the municipality of Jataí – GO, Brazil. The seeds were then dried in a forced air circulation oven at controlled temperatures of 40, 50, 60 and 70 °C until a final moisture content of 12 ± 1% d.b.. The seeds were stored in kraft paper packages for six months under laboratory conditions and evaluated for: moisture content, electrical conductivity, emergence in sand, emergence speed index, seedling length, seedling dry matter and accelerated aging. Parameters such as emergence, accelerated aging and seedling length decreased along storage, whereas electrical conductivity increased over time.
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Ferraz, Denes, Werther Pereira Ramalho, and Luís Sérgio Rodrigues Vale. "Methods for overcoming dormancy and seed storage of Enterolobium contortisiliquum." Acta Scientiarum. Agronomy 41, no. 1 (2019): 42602. http://dx.doi.org/10.4025/actasciagron.v41i1.42602.
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The production of forest species that present seedlings with exogenous dormancy is limited by low germination rates, which can be overcome by rupturing or weakening the tegument. This study aimed to evaluate the efficiency of low-cost methods of overcoming dormancy and determine the effect of storage on the seeds of Enterolobium contortisiliquum. Four storage periods were used, freshly collected seeds (0), stored for 6, 12 and 18 months, and four methods were applied, mechanical scarification and immersion in water (EI), mechanical scarification (ES), immersion in water (IM) and control (TE). We obtained the percentage values of seedling emergence (EP) and the mean emergence time (TME). Conductivity and moisture were obtained as indicators of the physical and physiological quality of seeds, respectively. Variations in moisture content and electrical conductivity during storage did not have effect on the percentage of germination. EI treatment was more efficient for overcoming dormancy than the ES was, regardless of storage time. IM and TE treatments did not lead to germination. EI was the most efficient treatment for decreasing the TME. The results indicate that alternative and low-cost methods are efficient at overcoming exogenous dormancy and thus can be used to optimize the process of seedling production.
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Rostad, Halvor, Aksel Granhus, Inger Sundheim Fløistad, and Svein Morgenlie. "Early summer frost hardiness in Picea abies seedlings in response to photoperiod treatment." Canadian Journal of Forest Research 36, no. 11 (2006): 2966–73. http://dx.doi.org/10.1139/x06-167.
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According to previous studies, short-day (SD) treatment may increase frost hardiness in Picea abies (L.) Karst. seedlings during shoot elongation the next year. The purpose of this study was to examine how timing of the SD treatment affects spring frost hardiness. The following four treatments were applied in the first growth period: natural photoperiod (Nat), or SD treatment (8 h light : 16 h dark) either 14–28 July (SD1), 28 July – 11 August (SD2), or 11–20 August (SD3). After 125 days in cold storage (October–January), the seedlings were transferred to forcing conditions (20–25 °C, 24 h light) followed by freezing tests at –3, –5, –7, and –9 °C when most seedlings had reached bud break stage 7 (Krutzsch index). Seedling height measurements and analyses of carbohydrate status, nitrogen concentration, and dry mass of needles after cold storage were done to examine the treatments' impact on seedling quality. SD treatment reduced visual freezing injury to current- and first-year shoots. Mortality occurred at –7 and –9 °C and was significantly higher in the Nat and SD1 treatments (43% in both) than in the SD2 (23%) and SD3 (15%) treatments. Seedlings from the late SD treatments also showed better height growth and developed more shoots from dormant buds after freezing to –3 and –5 °C. Collectively, these findings demonstrate the importance of proper timing of the SD treatment in relation to the seedlings' natural growth rhythm.
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Chipilski, Radoslav, Irina Moskova, Albena Pencheva, and Konstantina Kocheva. "Field priming with cytokinins enhances seed viability of wheat after low temperature storage." Plant, Soil and Environment 67, No. 2 (2021): 77–84. http://dx.doi.org/10.17221/524/2020-pse.
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Field experiments were conducted with two winter wheat cultivars that were primed with 6-benzylaminopurine (6-BA) or kinetin at the concentration 10 mg/L twice during the grain filling stage. After priming, wheat physiological parameters were measured in the field, and the analysis of yield was performed after harvest. Harvested seeds were subjected to low temperature storage for 12 months at –18 °С simulating conservation conditions in genebanks. In field experiments, treated plants exhibited up to 14% higher productivity, higher fresh and dry weight, and chlorophyll content index of flag leaves. Priming significantly improved germination, seedling vigour and growth parameters. In 5-days-old seedlings developed from low temperature stored seeds of field primed plants, the average accumulation of malondialdehyde and H<sub>2</sub>O<sub>2</sub> was estimated 25% lower, which contributed to higher cell membrane stability. These results correlated positively with growth characteristics of 15-days-old seedlings. The stimulating action of cytokinin priming was more pronounced in the modern cv. Geya-1 compared to the older cv. Sadovo 772 and could be attributed to improved anti-aging mechanism connected with better protection against oxidative damage.