Academic literature on the topic 'Seedling tubes'

Abstract Survival, height, and diameter growth of seedlings were evaluated for three years after planting using five types of tree shelters and seven species: green ash (Fraxinus pennylvanica), northern red oak (Quercus rubra L.), pin oak (Q. palustris), American sycamore (Platanus occidentalis), black walnut (Juglans nigra L.), flowering dogwood (Cornus florida), and eastern white pine (Pinus strobus). Differences in shelter environments were measured, including: light transmission measured as percent photosynthetic photon flux, ratio of red:far red (r:f-r) light from the red and far-red wavelengths, and air temperature inside the tubes. The differences seen in seedling survival were not significant (p < 0.05) for the presence or type of tree tube, with an average survival of 96% for all but two species. For most species, seedlings grown in high light-transmitting tubes with proportional r:f-r ratio light showed superior height growth (e.g., Miracle Tube, Tree-Pro, and Protex). Diameter growth generally decreased in shelters. Sycamore showed no significant benefit from the use of tubes. The lowest diameter increments were seen using Tubex brown and Mesh Guard shelters, which had low light transmission with high r:f-r ratio and mechanical damage, respectively. Light transmission in translucent tree tubes was within the ranges found in open canopy forest, but the proportion of growth-promoting far-red wavelength was generally lower. In tubes with higher light transmission, r:f-r ratio is closer to natural ranges for that light level. For tubes with lower light transmissivity, this information suggests that seedling height growth might be improved if red wavelengths were blocked more strongly.North. J. appl. For. North. J. Appl. For. 22(2):102–110.

The emerald ash borer (Agrilus planipennis Fairmaire; EAB) is an invasive insect that causes mortality of trees in the genus Fraxinus, creating canopy gaps that may facilitate invasion by exotic plants. Planting native tree seedlings under EAB-infested Fraxinus may accelerate succession and preclude invasive plant expansion; however, the effectiveness of this approach has not been experimentally tested. We assessed understory seedling planting of Quercus rubra, Carya laciniosa, and Juglans cinerea in EAB-infested forests, where the invasive shrub Lonicera maackii (Amur honeysuckle) was removed. We tested whether the use of plastic tree shelters (“tree tubes”) or planting season (fall versus spring) contributed to the success of the reforestation plan by measuring growth rates (cm/yr) and survivorship two and seven years after planting. After seven years, seedling survivorship was <25% for all species and planting techniques. Quercus rubra exhibited poor survivorship with one seedling surviving to the conclusion of the experiment. Juglans cinerea and C. laciniosa had higher survivability and growth rates than did Q. rubra after two and seven years. Effects of tree tubes were weak and temporary. After 2 years, Q. rubra seedling survivorship was higher in tree tubes; however, by the end of the experiment 29 of the 30 Q. rubra seedlings in tree tubes had died. Juglans cinerea seedlings grew faster when planted in the fall compared to the spring, but overall survivorship of these seedlings was unaffected by planting season. Neither the use of tree shelters nor the planting season contributed to the growth or survival of C. laciniosa seedlings. In summary, our results indicate that seedling planting of Carya and Juglans may be a useful way to increase biodiversity in regenerating forests; however, the resource-expensive processes of over-wintering seedlings and using tree shelters may not increase the success of reforestation efforts.

ABSTRACT The use of quality seedlings is important in establishing a productive coffee crop. However, the most widely used method to produce coffee seedlings is time consuming (6-12 months) and lacks new production technologies. This study aimed to assess the use of fertigation and a growth regulator in the production of coffee seedlings, in order to develop a system faster than the conventional method. For that, Topázio coffee cultivar seeds were pre-germinated and planted in tubes filled with substrate (composted pine bark), in a protected nursery. A randomized block design was used, in a 4 x 2 (fertigation levels x the use or not of growth regulator) factorial scheme, with four replications. Daily fertigation positively influenced all the growth variables evaluated. The foliar spraying of the growth regulator had little effect on seedling growth. When compared to the conventional system described in the literature, the coffee seedling production system described here reduced, by around 60 days, the production time and enables a large-scale production.

The use of high quality seedlings is a key factor for the success of vegetable production, and the container size and design characteristics of the containers are important determinants of seedling quality. A experiment was conducted to investigate the effects of different container types on quality of cucumber seedlings (Cucumis sativus L., cv. Aodai Melhorado). Seedlings were grown in a seedling nursery with shadow protection at 50% in different containers: (i) 128-cell trays; (ii) 64-cell trays; (iii) tubes of 120 cm3; and (iv) plastic cups of 180 cm3. The plant height, diameter, root length, shoot dry matter, root dry matter, sturdiness quotient (SQ), shoot:root dry matter ratio (SRR) and Dickson quality index (DQI) were measured at 21st days after sowing. The production of cucumber seedlings in 64-cell trays, polypropylene tubes of 120 cm3 and plastic cups of 180 cm3 resulted in the highest values for the majority of investigated traits. The tray of 128-cells results in lower quality cucumber seedlings compared to other containers. Tubes with volume of 120 cm3 and plastic cups with volume of 180 cm3 are more appropriate containers for the production of high quality cucumber seedlings. The DQI is a good indicator of the standard of quality of cucumber seedlings.

A method of growing newly germinated seedlings in small tubes was developed to enable the study of early root elongation. Three newly germinated alfalfa (Medicago sativa L.) seeds were placed in tubes of various diameters and volumes filled with sand at several moisture tensions, and the tubes were sealed to prevent moisture loss. For the first 4 d, roots elongated at approximately 8 mm d−1 in polyvinyl chloride plastic tubes holding 60 mL of sand with a moisture tension of 100 kPa or less. After the initial 4 d, root elongation continued at a slower rate, and branch roots developed before termination of the experiments at 12 d after germination. At a moisture tension of 300 kPa, root elongation was markedly less than at 100 kPa, and was zero at moisture tensions of 500 kPa or greater. In round-bottom glass tubes, root elongation rate was significantly slower in a sand volume of 23 mL than in 65 mL, and there was evidence of slower seedling development as the diameter of the tubes was reduced from 20 to 15 mm. Wax plus masking tape or nylon tape were equally effective in sealing the sides of tubes formed from the two halves of a plastic pipe previously cut lengthwise. A plastic cap plus wax was significantly better than plastic film for sealing the bottom ends of plastic tubes. Neither aeration nor replacement of water with a dilute nutrient solution had an effect on root elongation of young seedlings. Key words: Alfalfa, seedling, root elongation, moisture stress, Medicago sativa

Avaliou-se a produção de mudas de Eucalyptus urophylla, E. camaldulensis e Corymbia citriodora em blocos prensados e em dois modelos de tubetes por meio da avaliação da sobrevivência e do crescimento inicial das mudas. A sobrevivência foi avaliada aos sessenta dias e o crescimento inicial em altura e diâmetro ao nível do solo ao décimo mês pós-plantio. Foi utilizado o delineamento em blocos casualizados com cinco repetições. Mudas de E. urophylla e E. camaldulensis produzidas nos blocos e nos dois modelos de tubetes e mudas de C. citriodora produzidas nos dois modelos de tubetes obtiveram altas taxas de sobrevivência. Já as mudas de C. citriodora produzidas nos blocos apresentaram a menor média de sobrevivência no estudo. As médias de crescimento inicial das mudas de E. urophylla produzidas nos blocos e tubetes de 50 cm3 foram superiores às encontradas para mudas produzidas em tubetes de 35 cm3. Para E. camaldulensis e C. citriodora, as médias de crescimento inicial foram semelhantes nos três recipientes. Recomenda-se a produção de mudas de E. urophylla em tubetes de 50 cm3 ou em blocos prensados. As mudas de E. camaldulensis podem ser produzidas nos três recipientes, e C. citriodora nos tubetes de 50 e 35 cm3.Palavras-chave: Qualidade de mudas; desempenho pós-plantio. AbstractPerformance in the field of Eucalyptus urophylla, Eucalyptus camaldulensis and Corymbia citriodora produced in different containers. We evaluated the production of Eucalyptus urophylla, E. camaldulensis and Corymbia citriodora in pressed blocks and two tubes models by the evaluation of survival and early growth of seedlings. We assessed survival at sixty days and initial growth in height and diameter at ground level to the tenth month after planting. We used a randomized block design with five replications. Seedlings of E. urophylla and E. camaldulensis produced in blocks, and in two tube models, and seedlings of C. citriodora produced in two tube models had high survival rates. On the other hand, seedlings of C. citriodora produced in blocks presented the lowest survival mean. The initial seedling growth mean of E. urophylla produced in blocks and tubes of 50 cm3 were higher than for seedlings grown in tubes of 35 cm3. In relation to E. camaldulensis and C. citriodora the average initial growth were similar in the three containers. Production of seedlings of E. urophylla is recommended in tubes of 50 cm3 or in compressed blocks, E. camaldulensis seedlings can be produced in three containers, and C. citriodora in tubes of 50 and 35 cm3.Keywords: Seedling quality; post-planting performance.

Abstract We tested the ability of individual tree seedling protectors to deter mountain beavers from damaging Douglas-fir seedlings. Using captive mountain beavers in field pens, we tested 20 products representative of a wide range of barriers suitable for protecting individual tree seedlings from rodent damage. Eleven products protected 95% or more of seedlings from damage. Tree shelters and fabric shelters provided the most protection; 98 and 95% of seedlings were undamaged, respectively. Rigid mesh protector tubes protected 81% of seedlings, and protection netting protected 55% of seedlings. Purchase prices varied widely; protection netting was least expensive, followed by rigid mesh tubes, fabric shelters, and tree shelters. Seedling growth was greatest within fabric shelters and plastic tree shelters. Materials used to construct 15 of the 20 tree protectors were tested using captive mountain beavers in small sheltered pens. Here, samples of barrier materials were used to block access to a favored food. The four materials that excluded all test animals in all trials were from unvented tree shelters with solid seamless walls. Seven materials failed to exclude any animals in the sheltered-pen trials.

The aim of this study was to verify if a growth reduction of yellow passion fruit seedlings’ growth morphophysiology and quality could be changed by paclobutrazol applied through seedling immersion. The experiment was conducted in a greenhouse, with seedlings grown in polyethylene tubes (290 cm3), with substrate. At 40 days after sowing, the seedlings were immersed in an aqueous solution of paclobutrazol at concentrations of 0, 50, 100, 150, and 200 mg L-1. The experiment was conducted in a randomized block design, with five treatments (paclobutrazol concentrations) and four replicates. At 15 and 30 days after treatment, growth characteristics were evaluated. At the end of the assay, destructive evaluations related to mass determination, total leaf area, and seedling quality index were performed. Paclobutrazol treatment induced restrictions in seedling growth, except for fresh and dry mass of root and total fresh mass. Based on these characteristics, the increase in values induced by paclobutrazol was verified. The seedling quality, defined by the major value of the Dickson quality index and a smaller robustness index, was higher when submitted to paclobutrazol treatment.

Cucumber crop was evaluated for the influence of seedling age on its production during two consecutive years of 2011 and 2012 on experimental area of National Agricultural Research Center (NARC), Islamabad, Pakistan. Nursery of cucumber was raised in polythene tubes under protected structures (walk-in tunnels). Seedling transplantation of three different ages viz., 30, 45 and 60 days were compared with direct seeding in the open field. Seedling transplantation produced fruits earlier (22 days), increased availability period (41 days) as compared to direct seeded crop. Transplantation of 45 and 60 days old seedlings produced higher fruits yield of cucumber with higher gross return and gross margin. The results revealed that transplantation of 45 to 60 days old seedlings was suitable for achieving fruits earlier and profitable cucumber production technology.SAARC J. Agri., 13(2): 214-221 (2015)

The spermosphere model showed very high seedling-to-seedling variation in the ability to induce acetylene-reducing activity in bacteria associated with wheat roots. Several seedlings failed to induce nitrogenase in N2-flxing strains of Bacillus and Azospirillum brasilense. Increasing the concentration of yeast extract or the inoculum density, adding NaOH solution in the side arm of Pankhurst tubes to absorb CO2, and vernalizing the seeds did not increase the percentage of nitrogenase-positive tubes and did not decrease variation in acetylene-reducing activity. Increasing the incubation temperature from 25 to 27 °C induced nitrogenase in Bacillus, but not in Azospirillum strains; at 30 °C nitrogenase was also induced in Azospirillum strains. It appears that the spermosphere model is not a practical technique for screening nitrogenase induction by wheat, unless the use of genetic lines of unimpeachable uniformity can provide control of variability.

A restauração florestal em matas ciliares é de suma importância para os processos de recuperação de áreas degradadas e conectividade da flora e fauna. O presente experimento foi instalado às margens do reservatório de Borborema - SP, com o objetivo de averiguar o acúmulo de biomassa de trinta espécies arbóreas nativas frente a dois sistemas silviculturais e dois tipos de recipientes de produção de mudas. O ensaio foi instalado em duas áreas com umidade de solo distintas, sendo uma mais seca, sem encharcamento mesmo em períodos chuvosos, outra mais úmida, com encharcamento. Os sistemas de manejo testados foram: i) convencional e operacionalmente utilizado pela empresa AES-Tietê; e ii) potencial, com maior nutrição e maior controle de mato-competição. Os recipientes de produção de mudas testados foram o tubete pequeno, com 56 cm3; e tubete grande com 290 cm3. O estudo foi instalado em fatorial 2x2, com os fatores Manejo e Tubete e dois níveis de cada fator (potencial e convencional, e tubete pequeno e grande) com quatro repetições na área seca. Não ocorreu interação entre os fatores estudados. Na área seca, o tratamento que utilizou tubete grande e manejo potencial apresentou diâmetro e altura 30% superiores que o tratamento que utilizou tubete pequeno e manejo convencional, e uma superioridade em 70% no Índice de Área Foliar e 62% no acúmulo de biomassa aos dois anos. O tubete grande apresentou sobrevivência de plantio 13% superior ao tubete pequeno e ganho em biomassa média das mudas de 43%. O manejo potencial apresentou área basal superior ao manejo convencional (6,23 versus 4,09m2ha-1) e IAF superior (1,28 versus 0,58 m2m- 2). Na área úmida, as zonas de alagamento baixo, médio e total apresentaram área basal decrescentes de 4,4 m2 ha-1, 2,6 m2 ha-1 e 0,9 m2 ha-1. Com esses resultados é possível concluir que as espécies nativas possuem capacidade de melhor desempenho quando produzidas em tubetes maiores e sob sistema silvicultural mais intensivo e que o alagamento (falta de aeração da raiz) é um estresse ambiental que requer melhor compreensão por ser altamente limitante à restauração com as espécies tradicionais.<br>Riparian forest restoration is extremely important for the processes of degraded areas restoration and connectivity of flora and fauna. This study was established on the Reservoirs of the Borborema Lake - SP, with the purpose to determine the biomass accumulation of thirty tree species native to two silvicultural systems and two sizes of hard plastic tubes for seedling production. This trial was conducted in two areas with different soil moisture, a more drought, without flooding even during rainy periods, and another more humid, with frequent flooding. The following silvicultural systems were tested: i) traditional, one who is operationally used by the AES Tiete company; ii) potential, with greater nutrition and greater weed control. The containers for seedlings production tested were small hard plastic tubes with 56 cm3, and large hard plastic tubes with 290 cm3. The study was carried out in a factorial 22, with factors such as silvicultural systems and sizes of recipients, being two levels of each factor (potential, traditional, small and large hard plastic tubes) and four replications in a dry area. There was no interaction between the factors studied. In dry area the treatment that used large hard plastic tubes and potential silvicultural system showed diameter and height 30% higher than the treatment that used small hard plastic tubes and traditional silvicultural system, and also a superiority of 70% for the Leaf Area Index and 62% for biomass to two years. The seedlings produced in large hard plastic tubes showed survival at planting 13% higher than those grown in small hard plastic tubes. Was also observed that the mean biomass of seedlings was 43% higher for treatment of large tubes. Potential silvicultural system showed basal area greater than the traditional silvicultural system (6.23 vs. 4.09 m2 ha-1) and higher LAI (1.28 vs. 0.58 m2 m-2). In the wetlands, the zones of low, medium and high flooding frequency showed decreasing basal area of 4.4 m2 ha-1, 2.6 m2 ha-1 and 0.9 m2 ha-1. With these results we can conclude that native species have a higher performance when produced in large hard plastic tubes and under more intensive silvicultural system and flooding (lack of aeration of the root) is an environmental stress that requires better understanding because it is factor highly limiting for the restoration with the traditional species.

Switchgrass (Panicum virgatum L.) is a warm-season perennial bunchgrass native to North America. In addition to its importance as a forage grass, it has promise as a biofuel crop. However, its use is limited because the grass is difficult to establish. Improving seedling vigor is one approach for improving establishment. The objectives of this study were to: 1) select for increased seedling mass through half-sib family selection; 2) calculate an estimate of heritability for seedling mass; and 3) determine the mode of pollination of switchgrass. One cycle of selection was completed using a half-sib methodology. Seedling mass was determined in a series of growth chamber studies. The seed was produced in different space planted field nurseries in the College Station, TX area. Mean seedling weight of the base population (C0) was 0.014 gm seedling-1, while the mean seedling weight from the C1 cycle of selection was 0.029 gm seedling-1. Unfortunately, bulked seed from the base population was old and did not germinate well. Therefore, a new base population was recreated, and the C0 seedlings from this population were heavier than the C1 seedlings, 0.020 and 0.016 gm seedling-1, respectively. The calculated heritability estimate was H2 = 0.6. Since the C0 and C1 nurseries were not grown on the same soil type, the lack of a positive response for seedling weight may be due to the different soil types. However, it may require another cycle of selection to determine if seedling mass can be positively impacted via half-sib selection. The mode of pollination of the species was determined by 1) observing pollen germination and tube growth in the pistils using fluorescent microscopy and 2) determining seed set with selfed plants. When self-pollinated, the pollen tubes never grew into the ovaries but when cross-pollinated the tubes readily grew to the micropyle. Also, when switchgrass plants were self-pollinated, viable seed were not produced. These findings indicate that switchgrass is highly self-sterile because a self-incompatibility mechanism prevents the pollen tubes from growing into the ovary of the same genotype.

Por ser a cultura anual de maior importância econômica no Brasil e apresentar custo elevado de produção, novas alternativas vêm sendo adotadas para diminuir os custos e obter batata-semente de boa qualidade. Com o objetivo de determinar os parâmetros de crescimento e a marcha de absorção de nutrientes a partir de plântulas do cv. Atlantic, para a produção de minitubérculos em sistema hidropônico, realizou-se esse trabalho no período de setembro a novembro de 2004 em Vargem Grande do Sul – SP, localizada na altitude 21°47’28”S e na longitude 046°53’04”W com 938 m de altitude. O delineamento experimental foi inteiramente casualizado, com sete tratamentos e quatro repetições. As plantas foram avaliadas e coletadas em intervalos semanais do início do desenvolvimento até a fase da colheita aos 53 dias após transplantio (DAT). As plantas coletadas foram separadas em sistema radicular, parte aérea e minitubérculos, secadas em estufa e pesadas em balança analítica para obtenção de peso do material seco. Após secagem foram moídas em moinho e submetidas à análise para obtenção da extração de nutrientes para os tratamentos. Os resultados obtidos indicam que o acúmulo dos nutrientes é influenciado pelas fases da cultura e peso do material seco total das plantas. A seqüência do acúmulo máximo dos macronutrientes para os tratamentos foi: K > N > Ca > P > Mg > S e para os micronutrientes foi: Fe > Mn > B > Zn > Cu. A seqüência de exportação dos macronuteintes foi: K > N > P > S > Mg > Ca e para os micronutrientes foi: Fe > Zn > Cu > B > Mn. O sistema hidropônico permitiu disponibilizar os nutrientes mais prontamente assimiláveis pelas plantas e conseqüentemente maiores taxas de multiplicação.<br>As potato is the most important economic annual staple crop in Brazil and its production involves high costs, new alternatives are being adopted to decrease costs and obtain high quality potato seed. This study aimed at determining the growth parameters and the uptake rate by seedling from cv. Atlantic to be used for minituber production in a hydroponic system. It was carried out from setember to november 2004 in Vargem Grande do Sul – SP, located in latitude 21°47’28”S and longitude 046°53’04”W at altitude 938 m. The experimental design was randomized blocks with seven treatments and four replications. The plants were collected and evaluated at weekly intervals from the initial development phase throughout harvesting phase, 53 days after transplanting (DAT). The plants were separated into root system, aerial part and minituber, dried in oven and weighed on an analytical scale in order to obtain the dry mass weight. After drying, the plants were grounded in a mill and submitted to analysis in order to obtain the nutrient extraction for each treatment. The results showed that the nutrient accumulation is influenced by the phase of the crop and the total dry mass weight of plants. The sequence of maximum macronutrient accumulation for the treatments was: K > N > Ca > P > Mg > S, and for the micronutrient it was: Fe > Mn > B > Zn > Cu. The sequence of macronutrient exportation was: K > N > P > S > Mg > Ca, and for the micronutrients it was: Fe > Zn > Cu > B > Mn. The hydroponic system allowed to make the nutrients more readly available to the plants, and, as a consequence, higher rates of multiplcation were observed.

O início do uso de tubete na década de 80 revolucionou os viveiros, e hoje mesmo apresentando desvantagens, é amplamente difundido em todo o setor florestal. Esses tubetes de polipropileno, derivado do petróleo, possuem dois problemas: uso de fonte não renovável e resíduos para descarte. Uma alternativa é a substituição por plásticos biodegradáveis. Os objetivos foram avaliar: (a) a taxa de decomposição do compósito; (b) o crescimento em altura e em diâmetro do colo (DAC); (c) a produção de biomassa aérea e radicular; (d) a concentração de nutrientes nos tecidos vegetais; (e) a eficiência do uso de condições ambientais e de atributos do solo para predizer a taxa de decomposição. Os estudos no processo seminal ocorreram no viveiro da ESALQ, em Piracicaba. Para estudar a taxa de decomposição do compósito, utilizaram-se formulações com 20 e 30% (p p-1) de pó de madeira. No verão e no inverno aplicaramse 150; 225 e 300 g de N m-3 de substrato na fertilização de base. A diminuição de massa foi mensurada a cada dez dias, até 90 dias. Avaliou-se se o crescimento em altura e em DAC de mudas seminais entre 40-90 dias após a semeadura, a produção de biomassa e concentração de macronutrientes aos 90. No plantio realizado na E.E.C.F. de Anhembi, em três blocos ao acaso avaliou-se o crescimento em altura, em DAC e de produção biomassa, aos 15, 30, 45, 60, 90 e 120 dias. Para as mudas clonais utilizou-se tubete e minitubete de polipropileno, tubetes na coloração normal e na coloração escurecida, e minitubete de compósito. O estudo realizou-se em quatro viveiros e quatro plantios de Eucalyptus sp. no estado de São Paulo. No viveiro utilizouse 4 blocos casualizados. O crescimento em altura e em DAC, e a biomassa foram mensurados aos 90 dias. O plantio clonal em três blocos ao acaso foi mensurado em altura, DAC e produção de biomassa aos 15, 30, 60, 90, 120 e 150 dias. A concentração de macronutrientes foi determinada aos 150 dias. Os dados foram submetidos aos testes de normalidade, de homogeneidade de variâncias, da Análise de Variância e de Tukey. A análise da relação entre as variáveis dependentes e independentes foi realizada por meio de análises de correlação e de regressão. O aumento da dose de N e do teor de madeira não proporcionou aumento da taxa de decomposição, ao contrário da estação climática. A abertura de fissuras causou deformação do tubete, dificultando o manejo. As mudas seminais, crescimento e biomassa não diferiram no verão. Os plantios seminais não diferiram em crescimento e em biomassa. No sistema clonal crescimento e biomassa diferiram nos viveiros. No plantio clonal, crescimento e produção de biomassa não diferiram em três sítios. Tubetes e minitubete de compósito não impediram o desenvolvimento do sistema radicular. A diminuição da massa dos tubetes ou minitubete de compósito, em produção de mudas seminais ou clonal, pôde ser prevista com alta precisão em função das condições ambientais. Da mesma forma nos plantios seminais. No plantio clonal pelo menos em dois sítios houve alta relação.<br>The introduction of plastic tubes on 80s revolutionized nurseries, and nowadays even with disadvantages, is widespread throughout the forest sector. These polypropylene tubes, derived from petroleum, have two problems: use of a nonrenewable resource and waste for disposal. An alternative is the substitution for biodegradable plastics. The objectives were to assess: (a) the rate of decomposition of the composite, (b) the growth in height and ground level diameter (DAC), (c) the production of stem and root biomass, (d) the concentration of nutrients on plant tissues, (e) the efficiency of use of environmental conditions and soil properties to predict the rate of decomposition. Studies in the seminal case occurred in ESALQ´s nursery, Piracicaba. To study the rate of decomposition of composite were used formulations with 20 and 30% (p p-1) of wood powder. During summer and winter were applied 150, 225 and 300 g N m-3 of substrate for base fertilization. Decrease in mass was measured every ten days until 90 days. We evaluated the growth in height and DAC from seedlings between 40-90 days after sowing, the production of biomass and macronutrients at 90. On planting, held at E.E.C.F. of Anhembi in three randomized blocks, were evaluated height growth, DAC and biomass production at 15, 30, 45, 60, 90 and 120 days. For cuttings, we used minitubes and tubes of polypropylene, and tubes with normal and darkened colors, and minitubes of composite. The study took place in four nurseries and four Eucalyptus sp. plantations at state of São Paulo. At nursery we used four randomized blocks. The growth in height, DAC and biomass were measured at 90 days. The clonal plantation in three randomized blocks was measured in height, DAC and biomass production at 15, 30, 60, 90, 120 and 150 days. The concentration of macronutrients was determined at 150 days. Data were tested for normality, homogeneity of variance, analysis of variance and Tukey test. Analysis of relationship between dependent and independent variables was performed using correlation and regression. The increase of N and wood content did not increase the rate of decomposition, unlike the weather season. Opening of cracks caused deformation of the tube, making it difficult to manage. The growth and biomass of seedlings did not differ on summer. Seminal plantations did not differ in growth and biomass. At clonal system, growth and biomass differed in nurseries. In clonal plantation, growth and biomass production did not differ at three sites. Minitube and tubes of composite did not prevent the development of root system. Decrease of tube or minitube of composite masses in production from seedlings or cuttings was predicted with high accuracy, as a function of environmental conditions. Likewise in seminal plantations. In clonal planting, at least two sites, showed high relation.

碩士<br>中華科技大學<br>健康科技研究所在職專班<br>104<br>The purposes of this study were to (l) confirm the effect of truffle inoculation on the seedlings growth of Cyclobalanopsis glauca, (2) observe the competition of Scleroderma areolatum to the mycorrhizae formed either by Tuber formosanom or T. indicum and (3) compare the competition of S. areolatum to the truffle cultured on YMT agar medium.After seedlings of C. glauca were inoculated with T. formosanum and T. indicum, the height growth, root collar diameter, top dry weight and root dry weight were much more significantly stimulated than those of non-inoculated seedlings. The T/R ratio of both Tuber-inoculated seedlings was significantly lower than those of non-inoculated seedlings. S. areolatum could form typical white ectomycorrhizae when it was inoculated to one year’s old C. glauca seedlings mycorrhized either with T. formosanum or T. indicum two months later after inoculation. The other uninoculated rootlets could be infected by the rhizomorph extended from white ectomycorrhiza. The rhizomorph could also infect the Tuber-infected ectomycorrhizae and finally enveloped it. Therefore, the inoculation of S. areolatum could reduce or impede the mycorrhiza formation formed by T. formosanum or T. indicum. The Tuber- mycorrhized C. glauca seedlings inoculated with S. areolatum two months later could also stimulate seedlings growth, as the seedlings were inoculated with truffle only.YMT agar medium was used to test the colony competition between T. formosanum and S. areolatum, T. formasanum and S. areolatum each other. The results showed that the colony growth of either T. formosanum or T. indicum grew much quicker than that of S. areolatum, and could impact the growth of S. areolarum. There was compete dominance for the colony growth of T. indicum, especially. The colony of T. indicum could cover the colony of S. areolatum at the end of the test. The colony of T. formosanum and S. areolatunn grew mixedly each other at the late stage. Neither the hyphal anastomoses of T. indicum and S. areolatum, nor the hyphal anastomoses of T. formosanum and S. areolatum were observed.

碩士<br>國立中興大學<br>農藝學系所<br>106<br>Sweet potato (Ipomoea batatas L.) is rich in nutritional value and easy to cultivate, can be cooked into a variety of food, convenience store roasted sweet potato is one of the most popular, but the size of the potato has a certain limit, 150-400 grams of the most appropriate size, this The weight of the sweet potato can also be sold to a higher price, but the farmers in the planting of sweet potato, the size of sweet potato is often unable to control, In this experiment, the application of Indole-3-butyric acid (IBA) in c.v. Tainong 57 and c.v. Tainong 66 sweet potato was carried out by increasing the unit of Sink and the photosynthesis Of the energy was evenly distributed to each root, field experiments found that the application of IBA containing root powder can make sweet potato in the early development of more differentiation of the root, At the later harvest, there were more tuberous lines, especially 15-150 g, but no significant effect on overall yield due to IBA-treated plants. The reason was that the nutrients were dispersed in too much sweet potato, resulting in the weight of each sweet potato Are reduced and no increase in overall production.

The results of studies of the influence of biologicals on the process of adaptation of microclonal potato plants to in vivo conditions in greenhouse and field conditions are presented. Treatment of plant material with biological products Planriz and PhytoDoctor promotes the adaptation of in vitro potato plants to in vivo conditions, increases the survival rate of up to 97,8% of potato seedlings and increases the yield of mini-tubers.