Academic literature on the topic 'Seedling vigor'
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Journal articles on the topic "Seedling vigor"
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Ratnayake, Sunil, and David R. Shaw. "Influence of Seed Vigor, Herbicide Rates, and Incorporation Depths on Emergence and Seedling Development of Soybean (Glycine max)." Weed Technology 6, no. 4 (1992): 801–6. http://dx.doi.org/10.1017/s0890037x00036290.
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Imazaquin at 140, 280, and 420 g ai ha-1did not negatively affect seedling emergence from high vigor (98% germination) soybean seed but, coupled with a 5.0-cm herbicide incorporation depth, reduced seedling emergence from low vigor (67% germination) seed at all rates. Imazaquin at 420 g ha-1incorporated 5.0 cm deep reduced low and high vigor seedling height 36 and 28%, respectively. Imazaquin at all rates reduced root length from high vigor seed, whereas only the high rate reduced root length of seedlings from low vigor seed. Although 420 g ha-1metribuzin did not affect seedling emergence or root length, emerged seedlings were severely damaged when herbicide was incorporated to 5.0 cm. Trifluralin at 840 and 1680 g ai ha-1reduced emergence of low vigor seed when incorporated 5.0 cm deep. Trifluralin at 840 g ha-1did not affect seedling height or root length, but 1680 g ha-1reduced height of seedling from low and high vigor seed by 86 and 66% when incorporated 5.0 cm deep. Trifluralin at 1680 g ha-1reduced shoot dry weight of both types of seedlings only at the 5.0-cm incorporation depth.
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DIAS, MARCOS ALTOMANI NEVES, VITOR HENRIQUE VAZ MONDO, SILVIO MOURE CICERO, NAYARA ROBERTO GONÇALVES, and CIBELE APARECIDA TEIXEIRA DA SILVA. "VIGOR TESTS ASSOCIATION AS AN ALTERNATIVE FOR PRECISE AND EFFICIENT ASSESSMENT OF MAIZE SEED QUALITY." Revista Caatinga 28, no. 3 (2015): 93–99. http://dx.doi.org/10.1590/1983-21252015v28n311rc.
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ABSTRACT: This study aimed to associate two concepts of seed vigor testing, the aging and seedling growth using image analysis, providing a feasible and time-saving way to evaluate maize seed vigor in large scale. For this purpose, five seed lots with different vigor levels from two single hybrids were used. The seeds were characterized by moisture content, germination, seedling emergence, seedling emergence speed index, accelerated aging and cold tests. The treatments were composed by varying the accelerated aging periods (0, 24, 48, 72 and 96h) and seedling growth periods (48 and 72h), before submitting the seedlings to image analysis for seedling root length measurement. Considering the results obtained, the adaptation of accelerated aging test by reducing the aging duration from 96h to 48h, and the replacement of the germination test by seedling root length measurement using image analysis could be considered a potential tool for maize seed vigor assessment. The treatments composed of 48h of seed aging followed by seedlings root length measurements using image analysis provided reliable data, compared to traditional vigor tests and it could be considered an efficient and time-saving approach, associating two different concepts of seed vigor analysis.
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Alvarenga, Renata Oliveira, and Julio Marcos-Filho. "Vigor evaluation of stored cotton seeds, including the Seed Vigor Imaging System (SVIS®)." Journal of Seed Science 36, no. 2 (2014): 222–30. http://dx.doi.org/10.1590/2317-1545v32n2944.
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This study aimed at evaluating efficiency of different tests to assess cotton seed vigor during storage, including the SVIS® software. Thereby, five cotton seed lots (cv. BRS 293) were stored under controlled environmental conditions of low temperature and relative humidity (10 ºC; 30% RH), and under non-controlled laboratory environmental conditions, for eight months. Evaluations of germination, first germination count, cool germination, accelerated aging test traditional and with saturated salt, field seedling emergence and seedling imaging automated analysis - SVIS® were performed before and after every two storage months. It was concluded that the cool germination, accelerated aging (traditional and with saturated salt), field seedling emergence and seedlings length (SVIS®) are adequate parameters for assessing vigor of cotton seeds during storage, and that both accelerated aging and SVIS® (vigor index and seedlings length) present enough sensitivity to identify changes on seed vigor during storage.
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Silva, Jackson Araújo da, André Dantas de Medeiros, Daniel Teixeira Pinheiro, et al. "Semi-automated Phenotyping of Soybean Seedlings and Its Relation With Physiological Seed Quality." Journal of Agricultural Science 11, no. 16 (2019): 1. http://dx.doi.org/10.5539/jas.v11n16p1.
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The development of procedures enabling agility and effectiveness to the analyses of seed vigor are great advances for the seed research field. The aim of this paper was to evaluate the efficiency of the Seedling Analysis System (SAPL®) to seedling phenotyping and determining the physiological potential of soybean seeds, in comparison with the information provided by traditional vigor tests recommended for this species. The characterizing of physiological potential of the seed lots was carried out based on germination tests, first germination count, seedling emergence, cold test, and electrical conductivity. The results of these tests were compared with the data obtained using image analysis with the software SAPL®, which was used to evaluate the development seven- day old seedlings. It was obtained the length of shoot, primary root and entire seedling, growth rates, uniformity, vigor index and corrected vigor index, based on different variations of the growth/uniformity ratio, namely 80:20, 70:30, 60:40, in the vigor index calculation. Analysis of images of the seven-day old seedlings using SAPL® enables phenotyping of soybean seedlings and is a consistent and promising alternative for identifying differences in vigor between soybean seed lots, equivalent to that found with traditional vigor tests.
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Mellish and B. Coulman, A. "Seed weight, emergence and seedling vigour of four tetraploid crested wheatgrass populations." Canadian Journal of Plant Science 83, no. 1 (2003): 69–70. http://dx.doi.org/10.4141/p01-119.
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This study compared the seed weight, and seedling emergence vigor of four tetraploid crested wheatgrass populations. S9240H, a population selected for seed size, had larger seeds than the cultivars Kirk and CD-II. Heavy seeds had greater emergence and heavier seedlings than light seeds, but there were no differences in these characters among the four populations. Key words: Crested wheatgrass, seed size, seedling vigour
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Hu, Bizhen, Mark A. Bennett, and Matthew D. Kleinhenz. "A New Method to Estimate Vegetable Seedling Vigor, Piloted with Tomato, for Use in Grafting and Other Contexts." HortTechnology 26, no. 6 (2016): 767–75. http://dx.doi.org/10.21273/horttech03485-16.
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The primary objective of this study was to test an improved method for estimating vegetable seedling vigor, which is important in grafting and other contexts. The study was also designed to test correlations between destructive and nondestructive measures of seedling growth and the effect of tomato (Solanum lycopersicum) rootstock and scion seedling vigor on graft success. Emergence and biomass accumulation and distribution of 18 tomato rootstock and five scion cultivars were monitored in the greenhouse through 18 days after sowing using seven destructive and nondestructive measures; growing conditions were also monitored. Plant and environmental data were used: 1) to develop cultivar growth curves, rank-sum values, and multicomponent seedling vigor values, and 2) to test correlations between percent canopy cover and other foliar measures. Also, seedlings representing all 90 rootstock–scion combinations and their associated seedling vigor values were cleft-grafted using accepted methods and grafted-plant survival was evaluated 2 weeks later. The experiment was conducted twice. Overall seedling vigor and its components differed significantly between runs of the experiment and among cultivars, although most cultivars had similar rankings (relative vigor) in both runs. Rank-sum and seedling vigor values ordered cultivars similarly. However, the range of cultivar seedling vigor values (3–11,504) greatly exceeded the range of rank-sum values (4–92). Correlations between destructive and nondestructive measures were significant. Graft success did not differ among cultivar combinations. We conclude that 1) the method to estimate seedling vigor described herein is useful in grafting and other contexts, including when discerning cultivar and other treatment effects, 2) nondestructive measures can substitute for some destructive ones, and 3) graft success in tomato is unrelated to rootstock and scion seedling vigor, provided proper grafting and healing techniques and commercial cultivars are used.
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Ganigara, Basavaraj S., Doddabasawa, Hanumanthappa Dasanahalli, Shakuntala N.M, Umesh Hiremath, and Maruti.K. "Influence of Potting Mixture and Sowing Orientation on Physiological Parameters of Balanites roxburghii (Linn.)." Journal of Scientific Research and Reports 30, no. 11 (2024): 483–93. http://dx.doi.org/10.9734/jsrr/2024/v30i112576.
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Balanites roxburghii (Linn.) species has been classified under the category of endangered plant species due to excessive exploitation for a variety of purposes, which has necessitated conservation of this tree species, and hence a study was conducted to investigate the effect of potting mixture and sowing orientation on early growth performance and vigour. Seeds were subjected to seven potting mixtures: M1: Soil:Sand:FYM 2:1:1, M2: Soil:Cocopeat:FYM 2:1:1,M3: Soil:Cocopeat:Vermicompost 2:1:1, M4: Soil:Paddy Husk:FYM: 2:1:1, M5: Soil:Paddy Husk:Vermicompost: 2:1:1, M6: Soil: Saw Dust:FYM 2:1:1, and M7: Soil:sawdust: Vermicompost 2:1:1. The seeds were sown in three orientations. S1: Seeds laid vertically with stalk end downwards; S2: Seeds laid horizontally and S3: Seeds laid vertically with stalk end upwards. Physiological parameters considered for assessment were seed germination, days taken for germination, seedling length, and seedling vigor index. A completely randomized design was used for the experiment. The results showed that there is a significant difference among seven different potting mixtures and three orientations. At the end of 8 weeks, the seed is sown horizontally in the potting mixture Soil:cocopeat:vermicompost (2:1:1) has recorded the germination percentage of 71.67%, 37 days taken for seed germination, highest seedling length (64 cm), and highest seedling vigour index (4561). The high-vigor seedlings may survive even in unfavorable conditions, with high survival rate compared to others. The study recommended that to obtain high-vigor Balanites roxburghii seedlings, seeds may be sown in potting mixture soil: cocopeat: vermicompost (2:1:1) with horizontal orientation.
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Chiquito, Adrielle Aparecida, Francisco Guilhien Gomes Junior, and Julio Marcos-Filho. "Assessment of physiological potential of cucumber seeds using the software Seedling Vigor Imaging System® (SVIS®)." Revista Brasileira de Sementes 34, no. 2 (2012): 255–63. http://dx.doi.org/10.1590/s0101-31222012000200010.
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The software Seed Vigor Imaging System (SVIS®), has been successfully used to evaluate seed physiological potential by automated analyses of scanned seedlings. In this research, the efficiency of this system was compared to other tests accepted for assessing cucumber (Cucumis sativus L.) seed vigor of distinct seed lots of Supremo and Safira cultivars. Seeds were subjected to germination, traditional and saturated salt accelerated aging, seedling emergence, seedling length and SVIS analyses (determination of vigor indices and seedling growth uniformity, lengths of primary root, hypocotyl and whole seedlings). It was also determined whether the definition of seedling growth/uniformity ratios affects the sensitivity of the SVIS®. Results showed that analyses SVIS have provided consistent identification of seed lots performance, and have produced information comparable to those from recommended seed vigor tests, thus demonstrating a suitable sensitivity for a rapid and objective evaluation of physiological potential of cucumber seeds. Analyses of four-days-old cucumber seedlings using the SVIS® are more accurate and growth/uniformity does not affect the precision of results.
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Nguyen, Chi D., Jianjun Chen, David Clark, Hector Perez, and Heqiang (Alfred) Huo. "Effects of Maternal Environment on Seed Germination and Seedling Vigor of Petunia × hybrida under Different Abiotic Stresses." Plants 10, no. 3 (2021): 581. http://dx.doi.org/10.3390/plants10030581.
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Seed germination and seedling vigor can be affected by environmental cues experienced by the mother plant. However, information about how the maternal environment affects seed quality is scarce in ornamental plants. This study aimed to investigate the effects of two different maternal environments on the seed germination and seedling vigor of Petunia × hybrida under a variety of abiotic stresses. Petunia mother plants were grown in either a greenhouse during the summer months or an indoor controlled-temperature-and-light environment. Collected seeds were subjected to external stressors, including polyethylene glycol (PEG), sodium chloride (NaCl), high temperature, and abscisic acid (ABA), to determine seed germination percentage and seedling vigor. Results indicated that seeds harvested from the mother plants grown in a controlled environment germinated better than seeds harvested from the mother plants grown in the greenhouse when suboptimal germination conditions were applied. Additionally, the seedlings from the controlled maternal environment performed better in both ABA and salinity stress tests than the greenhouse seedlings. Interestingly, the greenhouse seedlings displayed less reactive oxygen species (ROS) damage and lower electrolyte leakage than the controlled environment seedlings under dehydration stress. The difference in germination and seedling vigor of seeds from the two different maternal environments might be due to the epigenetic memory inherited from the mother plants. This study highlighted the strong impact of the maternal environment on seed germination and seedling vigor in Petunia and may assist in high-quality seed production in ornamental plants.
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B. Supriya, Y. Bharathi, M. Rajender Reddy, and S. N. C. V. L. Pushpavalli. "Studies on variability parameters and correlations in Soybean (Glycine max) germplasm for seed quality characters." EMERGENT LIFE SCIENCES RESEARCH 09, no. 02 (2023): 159–67. http://dx.doi.org/10.31783/elsr.2023.92159167.
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Eighty-four soybean germplasm lines were tested for seed quality traits. The analysis of variance was performed and all the germplasm lines significantly differed for the characters viz., Percentage of germination, Seedling vigour index-I, Field emergence percent, Seedling vigour index-II, Electrical conductivity, and Moisture percentage. The correlation and variability studies were done for quality parameters in seed for eighty-four germplasm lines and results obtained were noted with high heritability and genetic advance along with large variability for Seedling vigor index-I, Seedling vigor index-II and electrical conductivity of seed leachates. The percentage of germination exhibited positive correlation with Seedling vigor index I and II along with field emergence and negative correlation with electrical conductivity. Genotypes GP-186, ASB-135, GP-142, GP-104, GP-145, ASB-114, ASB-15, ASB-104, GP-174, ASB-101, ASB-139, Aisb-50, Basara, Js-335 and Js-93-05 recorded superior seed quality parameters.
Dissertations / Theses on the topic "Seedling vigor"
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Ramirez, de Leon Hector. "Method of pollination and heritability for seedling vigor in switchgrass." Texas A&M University, 2005. http://hdl.handle.net/1969.1/2374.
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Switchgrass (Panicum virgatum L.) is a warm-season perennial bunchgrass native to North America. In addition to its importance as a forage grass, it has promise as a biofuel crop. However, its use is limited because the grass is difficult to establish. Improving seedling vigor is one approach for improving establishment. The objectives of this study were to: 1) select for increased seedling mass through half-sib family selection; 2) calculate an estimate of heritability for seedling mass; and 3) determine the mode of pollination of switchgrass. One cycle of selection was completed using a half-sib methodology. Seedling mass was determined in a series of growth chamber studies. The seed was produced in different space planted field nurseries in the College Station, TX area. Mean seedling weight of the base population (C0) was 0.014 gm seedling-1, while the mean seedling weight from the C1 cycle of selection was 0.029 gm seedling-1. Unfortunately, bulked seed from the base population was old and did not germinate well. Therefore, a new base population was recreated, and the C0 seedlings from this population were heavier than the C1 seedlings, 0.020 and 0.016 gm seedling-1,
respectively. The calculated heritability estimate was H2 = 0.6. Since the C0 and C1 nurseries were not grown on the same soil type, the lack of a positive response for seedling weight may be due to the different soil types. However, it may require another cycle of selection to determine if seedling mass can be positively impacted via half-sib selection. The mode of pollination of the species was determined by 1) observing pollen germination and tube growth in the pistils using fluorescent microscopy and 2) determining seed set with selfed plants. When self-pollinated, the pollen tubes never grew into the ovaries but when cross-pollinated the tubes readily grew to the micropyle. Also, when switchgrass plants were self-pollinated, viable seed were not produced. These findings indicate that switchgrass is highly self-sterile because a self-incompatibility mechanism prevents the pollen tubes from growing into the ovary of the same genotype.
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Magalhães, Stefânia Caixeta. "Disponibilidade de minerais, germinação e vigor de sementes de milho /." Jaboticabal : [s.n.], 2009. http://hdl.handle.net/11449/96797.
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Orientador: Francisco Humberto Dübbern de Souza<br>Banca: Roberval Daiton Vieira<br>Banca: Ana Dionísia da Luz Coelho Novembre<br>Resumo: A maioria dos métodos utilizados para estimar o vigor de amostras de sementes são qualitativos, portanto sujeito a erros do avaliador e de interpretação dos resultados. Metodologias quantitativas podem constituir alternativas mais confiáveis. Este trabalho teve como objetivo verificar se os níveis de alguns minerais armazenados nas sementes e disponibilizados durante etapas iniciais do processo de germinação constituem indicadores sensíveis e confiáveis do nível de vigor de sementes de milho. Vinte lotes de milho foram avaliados pelos testes de germinação, de condutividade elétrica, frio e de emergência da plântula. Após 12 h, 24 h, 36 h e 48 h do início da germinação, foram avaliados também as concentrações de fósforo inorgânico (Pi), fósforo, cálcio, potássio e magnésio por meio das técnicas de análise de injeção em fluxo (FIA) e de espectrometria de emissão ótica acoplada a plasma de argônio induzido (ICP-OES). Para estas análises, os minerais foram extraídos de amostras moídas com água a 95ºC, agitadas por 30 minutos e centrifugadas. Concluiu-se que as concentrações de Pi, P, Ca, Mg e K, extraídos pelo método da água aquecida, em sementes de milho híbrido após determinados períodos de germinação, permitiram agrupar amostras de lotes de sementes de forma idêntica à permitida pelos resultados obtidos com vários testes utilizados para avaliar o nível de vigor de amostras de sementes. Nesse trabalho isso foi verificado pelas avaliações dos níveis de Pi após 12 h e 48 h, de P após 12 h, 36 h e 48 h, de Ca após 24 h, 36 h e 48 h, de Mg após 36 h e 48 h e de K após 36 h e 48 h de germinação.<br>Abstract: Most of the traditional methods used for seed vigor evaluation are qualitative and, as such, prone to errors and results misinterpretation. Quantitative methods may constitute more reliable alternatives. The aim of this work was to verify if the concentrations of some minerals stored in the seeds and made available during the initial stages of the germination process constitute sensitive and reliable indicators of the level of corn seed vigor. Twenty seed lots were evaluated by means of the germination, electrical conductivity, cold and seedling field emergence tests. Additionally, the concentrations of inorganic phosphorus, phosporus, calcium, potassium and magnesium at 12 h, 24 h, 36 h and 48 h after the initiation of the germination process were also evaluated by means of the injection analysis in flow (FIA) and the spectrometry of optic emission coupled to plasma of induced argon (ICP-OES) techniques. For these analyses grinded seed samples were extracted with water at 95ºC, agitated for 30 minutes and centrifuged . It was concluded that the concentrations of Pi, P, Ca, Mg and K, extracted by the warm water method, in hybrid corn seeds after certain stages of the germination process, allowed the rating the of seed lots in a way similar to that obtained with the results of several other seed vigor tests. Specifically, this was achieved by evaluating the concentratrions of Pi after 12 h and 48 h, of P after 12 h, 36 h and 48 h, of Ca after 24 h, 36 h and 48 h, of Mg after 36 h and 48 h and of K after 36 h and 48 h after the beginning of the germination process.<br>Mestre
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Magalhães, Stefânia Caixeta [UNESP]. "Disponibilidade de minerais, germinação e vigor de sementes de milho." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/96797.
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magalhaes_sc_me_jabo.pdf: 624079 bytes, checksum: 37a746a1dac768fd2d8f6dcc974b9b8d (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>A maioria dos métodos utilizados para estimar o vigor de amostras de sementes são qualitativos, portanto sujeito a erros do avaliador e de interpretação dos resultados. Metodologias quantitativas podem constituir alternativas mais confiáveis. Este trabalho teve como objetivo verificar se os níveis de alguns minerais armazenados nas sementes e disponibilizados durante etapas iniciais do processo de germinação constituem indicadores sensíveis e confiáveis do nível de vigor de sementes de milho. Vinte lotes de milho foram avaliados pelos testes de germinação, de condutividade elétrica, frio e de emergência da plântula. Após 12 h, 24 h, 36 h e 48 h do início da germinação, foram avaliados também as concentrações de fósforo inorgânico (Pi), fósforo, cálcio, potássio e magnésio por meio das técnicas de análise de injeção em fluxo (FIA) e de espectrometria de emissão ótica acoplada a plasma de argônio induzido (ICP-OES). Para estas análises, os minerais foram extraídos de amostras moídas com água a 95ºC, agitadas por 30 minutos e centrifugadas. Concluiu-se que as concentrações de Pi, P, Ca, Mg e K, extraídos pelo método da água aquecida, em sementes de milho híbrido após determinados períodos de germinação, permitiram agrupar amostras de lotes de sementes de forma idêntica à permitida pelos resultados obtidos com vários testes utilizados para avaliar o nível de vigor de amostras de sementes. Nesse trabalho isso foi verificado pelas avaliações dos níveis de Pi após 12 h e 48 h, de P após 12 h, 36 h e 48 h, de Ca após 24 h, 36 h e 48 h, de Mg após 36 h e 48 h e de K após 36 h e 48 h de germinação.<br>Most of the traditional methods used for seed vigor evaluation are qualitative and, as such, prone to errors and results misinterpretation. Quantitative methods may constitute more reliable alternatives. The aim of this work was to verify if the concentrations of some minerals stored in the seeds and made available during the initial stages of the germination process constitute sensitive and reliable indicators of the level of corn seed vigor. Twenty seed lots were evaluated by means of the germination, electrical conductivity, cold and seedling field emergence tests. Additionally, the concentrations of inorganic phosphorus, phosporus, calcium, potassium and magnesium at 12 h, 24 h, 36 h and 48 h after the initiation of the germination process were also evaluated by means of the injection analysis in flow (FIA) and the spectrometry of optic emission coupled to plasma of induced argon (ICP-OES) techniques. For these analyses grinded seed samples were extracted with water at 95ºC, agitated for 30 minutes and centrifuged . It was concluded that the concentrations of Pi, P, Ca, Mg and K, extracted by the warm water method, in hybrid corn seeds after certain stages of the germination process, allowed the rating the of seed lots in a way similar to that obtained with the results of several other seed vigor tests. Specifically, this was achieved by evaluating the concentratrions of Pi after 12 h and 48 h, of P after 12 h, 36 h and 48 h, of Ca after 24 h, 36 h and 48 h, of Mg after 36 h and 48 h and of K after 36 h and 48 h after the beginning of the germination process.
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Németh, M. Barbara. "Pollen Performance and Seedling Vigor in Laboratory and Natural Populations of Clarkia Unguiculata (Onagraceae)." Miami University / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=miami1117816212.
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Sekharan, Soja. "Aged soybean (Glycine max [L.] Merrill) seeds – their physiology and vigor assessment." The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1135809134.
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Hatzig, Sarah Vanessa [Verfasser]. "Breeding for climate change : genetics and physiology of seed vigor, seedling vigor and early drought resistance in winter oilseed rape (Brassica napus L.) / Sarah Vanessa Hatzig." Gießen : Universitätsbibliothek, 2015. http://d-nb.info/108012019X/34.
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Godbey, Alan Lee. "Influence of phosphorus, sulfur, and molybdenum fertilization on the seedling vigor of selected legumes adapted to the Appalachian Region." Thesis, Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/50023.
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Legume establishment is difficult on many moderately acid, infertile soils in the humid northeastern United States. Legume seedling vigor as influence by P, S, and Mo fertilization was studied in order to determine fertilizer needs for improved establishment. A Gilpin silt loam was fertilized with 0, 22, 67, and 201 mg P kg⁻¹ in combination with 0, 22, 67, and 201 μg Mo kg⁻¹ in a greenhouse experiment in 1983. Legumes studied in this experiment were red clover (<i>Trifolium pratense</i>), white clover (<i>T. repens</i>), and birdsfoot trefoil (<i>Lotus corniculatus</i>). Field experiments with red clover, birdsfoot trefoil, and flatpea (<i>Lathyrus sylvestris</i>) were initiated in the spring of 1983 and 1984 using 0, 50, 150, and 450 kg P ha⁻¹, 0 and 60 kg S ha⁻¹, and 0 and 874 g Mo ha⁻¹. Seedling vigor as measured by plant height, trifoliate leaf count, dry weight, and trifoliate leaf area increased the greatest in the greenhouse using 22 mg P kg⁻¹ relative to the higher rates of P fertilization. Seedling vigor without applied P was poor, which clearly indicated the essential need for P in the early stages of legume growth. Molybdenum applied at 201 μg kg⁻¹ increased the growth of the greenhouse grown legumes the greatest above the 0 μg Mo kg⁻¹ rate within each added P treatment. Seedling vigor however, was not enhanced with Mo fertilization until the P deficiency was corrected. Field established legumes increased in height and dry matter yield the most using 50 kg P ha⁻¹ with respect to the additional increments of applied P, but the increase was not as great as that obtained in the greenhouse using 22 mg P kg⁻¹, This was attributed to a higher extractable P level before fertilization within the field experiments. Sulfur fertilization generally did not enhance seedling vigor in the field studies; although, red clover yield was increased using 60 kg S ha⁻¹ in the 1984 field experiment, Molybdenum applied at 874 g ha⁻¹ increased seedling vigor as measured by plant height and yield in the field experiments with or without P or S fertilization. Phosphorus uptake and Mo concentrations were increased in the plant tissues with either P or Mo fertilization.<br>Master of Science<br>incomplete_metadata
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Oliveira, Augusto Pedroso de. "Determinação da viabilidade e do vigor em sementes de seringueira." Universidade Federal de Goiás, 2012. http://repositorio.bc.ufg.br/tede/handle/tede/3208.
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Previous issue date: 2012-06-15<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES<br>To produce rubber tree (Hevea brasiliensis) seedlings in nursery, the collection is limited by the short fall of seeds, which have a rapid loss of their germinal power and no guarantee their viability. This forces the nurseryman to acquire a much larger seeds than really necessary. With the adoption of standards for the quality of seed, the minimum parameters established 70% viability being suggested for such confirmation the tetrazolium test, according Rules for Seed Analysis (RSA) and/or a practical test done from the visual analysis of the seed endosperm color named in this work of "visual test". Despite tetrazolium test be scientifically based, its realization is made improbable by many financial and temporal reasons. Thus, the visual test points as a low-cost alternative, high reliability and immediate result, since both tests are considered trusted, but only the test tetrazolium test is recognized. The subject proposed to verify the credibility of the "visual test" legitimizing or not its use in the field. The field is composed of rubber seed, formed with the adult clone GT1. The seeds were homogenised and stored on two conditions: braided polypropylene bags and transparent plastic bags with minimum thickness of 0.02 mm microdrill at top. In each bag, in three treatments were placed ten kilograms of seed and these were stored in natural conditions of temperature and humidity (25° C ± 5 °C). Was pulled randomly a bag of seeds of rubber tree of each storage type, to zero and fourteen days of storing lots. The tests: visual, sprouting in sand and tetrazolium, besides the determination of the degree of moisture. Tetrazolium and visual tests were highly correlated, and the visual test results before had as the best way to analyze the seeds, for its significant correlation in General with the tetrazolium test, and simplicity in its execution and instant result, which gives good lot projection nurseryman to be acquired by him.<br>A produção de mudas de seringueira (Hevea brasiliensis) em viveiro, é limitada pelo curto período de produção, queda e coleta das sementes, que possuem uma rápida perda do seu poder germinativo e nenhuma garantia da sua viabilidade. Isto obriga o viveirista a adquirir uma quantidade de sementes maior do que aquela realmente necessária. Com a aprovação de normas para o padrão de qualidade de semente, se estabeleceram parâmetros mínimos de 70% de viabilidade sendo sugerido para tal confirmação o teste de tetrazólio, segundo as Regras para Análise de Sementes (RAS) e, ou, um teste prático feito a partir da análise visual da cor do endosperma da semente nomeado no presente trabalho de “teste visual”. Apesar do teste de tetrazólio ser embasado cientificamente, sua realização é inviabilizada por diversos motivos de ordem financeira e temporal. Sendo assim, o teste visual aponta como uma alternativa de baixo custo, alta confiabilidade e resultado imediato, já que ambos os testes são considerados confiáveis, mas apenas o teste de tetrazólio é reconhecido. Nesta pesquisa objetivou-se verificar a credibilidade do “teste visual” legitimando ou não seu uso no campo. As sementes de seringueira foram coletadas no município de Goianésia, estado de Goiás. O campo de sementes é constituido de seringal adulto, formado com o clone GT1. As sementes foram homogeneizadas e armazenadas em duas condições: sacos de polipropileno trançado e sacos plásticos transparentes com espessura mínima de 0,02 mm, microperfurados na parte superior. Em cada saco, nos dois tratamentos, foram colocados dez quilogramas de sementes e estes foram armazenados em condições naturais de temperatura e umidade (25° C ± 5 °C). Foi retirado aleatoriamente um saco de sementes de seringueira de cada tipo de armazenamento, no tempo zero e aos quatorze dias de armazenamento dos lotes de sementes coletados. Foram realizados os testes: visual, de germinação em areia e de tetrazólio, além da determinação do grau de umidade. Os testes visual e de tetrazolio foram altamente correlatos. O teste visual apresentou a melhor forma de análise das sementes, por sua significativa correlação no geral com o teste de tetrazólio, além da simplicidade em sua execução e resultado instantâneo, o que confere ao viveirista uma boa projeção do lote de sementes a serem adquiridas para a produção de mudas de seringueiras
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Weerasooriya, Dilooshi Kumari. "Genetic analysis of interveinal chlorosis and reduced seedling vigor as related to agronomic performance in sorghum resistant to ALS inhibitor herbicides." Diss., Kansas State University, 2016. http://hdl.handle.net/2097/32896.
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Doctor of Philosophy<br>Department of Agronomy<br>Tesfaye T. Tesso<br>The lack of effective post-emergence weed control options is often highlighted as one of the major factors behind dwindling acreage under sorghum (Sorghum bicolor (L.) Moench) in the United States. The discovery of herbicide resistance sources in wild sorghum population and subsequent efforts to incorporate them into cultivated sorghum was received with much optimism to change weed management practices in sorghum. As the development of the technology advances, especially of the Acetolactate synthase (ALS) resistance, concerns over the temporary interveinal chlorosis and reduced seedling vigor in some of the resistant families became heightened. This thesis research is designed to shed light on the genetic basis of seedling chlorosis and assess its impacts on yield potential.
The study has three parts; the first part is focused on identifying the genetic causes and plant mechanisms associated with the chlorotic phenotype. ALS herbicide resistant sister-lines expressing normal and chlorotic phenotypes were analyzed via RNA sequencing at four time points during seedling growth. The study identified several variants of genes coding chloroplast precursors and those that cause epigenetic modifications. Once confirmed, genetic markers can be developed to track these gene variants in the breeding population and eliminate segregates genetically prone to chlorosis/yellowing.
The second part of the study focuses on assessing the effect of ALS resistance associated chlorosis on agronomic and nutritional parameters of sorghum inbred lines. A set of ALS resistant lines expressing different levels of the chlorotic phenotype were evaluated in replicated field trials and laboratory methods. Results showed that interveinal chlorosis delays flowering but does not have negative effect on yield and nutritional parameters with and without herbicide treatment. The last part addresses whether there is any yield drag that may be associated with herbicide resistance traits and foliar interveinal chlorosis. For this, we synthesized a large set (182) of hybrids from ALS resistant, ACCase resistant and regular (susceptible) seed and pollinator parents. The hybrids were then evaluated in three sets at multiple locations during the 2014 and 2015 crop seasons along with commercial checks. The results revealed that resistance to both herbicides do not cause any drag to grain yield. The traits also do not have any negative impact on grain and nutritional quality of resistant hybrids.
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Teixeira, Everton Felix. "Análise de imagens digitais na avaliação de plântulas de milho." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-25042005-155848/.
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A análise de imagens digitais tem grande potencial de uso na determinação do vigor de sementes. Associada ao teste de crescimento de plântulas, essa técnica possibilita a análise dimensional de imagens com rapidez e precisão. O resultado é a extensão total de cada plântula via quantificação computadorizada do comprimento de suas partes constituintes. Assim, o objetivo do trabalho foi estudar o vigor de lotes de sementes de milho, por meio do teste de crescimento de plântulas, utilizando-se a análise de imagens. Plântulas de milho (genótipo AG122) foram retiradas do germinador ao quarto dia de desenvolvimento e ordenadas sobre uma folha de poliéster transparente na superfície de um scanner para a captação das imagens. Desenvolveu-se uma rotina de processamento no programa Scil-Image para a análise das imagens digitais obtidas das plântulas. Houve medição computadorizada da extensão total, com a soma usual do comprimento do coleóptilo ao comprimento da maior raiz da plântula e, também, não usualmente, ao tamanho de todo sistema radicular. As plântulas foram mensuradas manualmente, visando a comparação com o método em estudo. Os resultados mostraram que a técnica digital possibilita a associação dos dados obtidos no processamento a eventuais diferenças de vigor existentes em lotes de sementes de milho, de maneira similar a outros métodos destinados à avaliação do vigor de sementes da referida espécie.<br>The image analysis has high potential use in seed vigor determination. Associated to the seedlings growing test, this technique is fast, precise and makes possible the dimensional image analysis. The result is the total extension of each seedling quantifying the length of their constituent parts. With the purpose of studying the corn seed lots vigor through the seedlings growing test, using the digital images analysis, the corn seedlings (AG122 genotype) were taken from the germination chamber at the fourth day of development and ordered over a sheet, made with a transparent polyester film, on a scanner surface to the images capture. A routine was developed to process digital images of seedlings into the Scil-Image software. There was a computational procedure to measure the total length, with the usual sum of coleoptile to the main root length of seedling and also, not usually, to all root system. The seedlings were measured manually, seeking comparison with the method in study. The results showed that the digital technique makes possible association of the data obtained in processing to eventual vigor differences existing in corn seed lots, in a similar way to the other methods appointed to seed vigor evaluation by referred species.
Books on the topic "Seedling vigor"
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Walls, Timothy W. The effects of soaking and low temperatures on germination and seedling vigor of snap beans (Phaseolus vulgaris L. cv. Slenderette). 1985.
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Aldous, David E., and Ian H. Chivers. Sports Turf and Amenity Grasses. CSIRO Publishing, 2002. http://dx.doi.org/10.1071/9780643090019.
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Sports Turf and Amenity Grasses is a comprehensive reference for anyone involved with the selection and maintenance of grasses used in sports and amenity areas in all areas. It provides a means to identify these grasses through keys, descriptions and photographs, and also provides detailed information on sowing, oversowing, stolonising and mowing heights. 
 The performance of each grass is assessed and detailed comments made on positive and negative aspects of its use. A grass’s tolerance to high temperature, frost, drought, shade, wet soil, salinity, low soil fertility, wear and close mowing is given in a table with each aspect rated. Further comments are made on how well it combines with particular grasses and on issues such as seedling vigour and sowing times.
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Leith, Phillip Edward Meric. Growth of Timothy (phleum Pratense L. ) Seed from Pollination to Maturity and Effect of Degree of Maturity at Harvesting and of Chilling upon Germination and Seedling Vigour. Creative Media Partners, LLC, 2021.
Find full textBook chapters on the topic "Seedling vigor"
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Burris, J. S. "Soybean Seedling Growth and Vigor." In World Soybean Research Conference III: Proceedings. CRC Press, 2022. http://dx.doi.org/10.1201/9780429267932-139.
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Vertucci, Christina W. "The Kinetics of Seed Imbibition: Controlling Factors and Relevance to Seedling Vigor." In Seed Moisture. Crop Science Society of America, 2015. http://dx.doi.org/10.2135/cssaspecpub14.c6.
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Mohapatra, Shibani, Sandeep Kumar Behera, Keshab Kumar Mohapatra, Alok Kumar Panda, and Ajay Kumar Mishra. "Advancing Rice Cultivation: Hydroponic Nurseries for Enhanced Resource Efficiency and Seedling Vigor." In Transition to Regenerative Agriculture. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-1421-9_7.
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Hatami, Mehrnaz. "Stimulatory and Inhibitory Effects of Nanoparticulates on Seed Germination and Seedling Vigor Indices." In Nanoscience and Plant–Soil Systems. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-46835-8_13.
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Dunand, R. T. "Enhancement of seedling vigor in rice (Oryza sativa L.) by seed treatment with gibberellic acid." In Progress in Plant Growth Regulation. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2458-4_102.
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Quilloy, Fergie Ann, Benedick Labaco, Carlos Casal, and Shalabh Dixit. "Crop Establishment in Direct-Seeded Rice: Traits, Physiology, and Genetics." In Rice Improvement. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66530-2_6.
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AbstractThe changing climate and water availability strongly affect the current state of agricultural production. While the global temperature rises, the occurrence of extreme climatic conditions becomes erratic. This current scenario has driven the development of rice varieties and cultivation practices that require less water and favor mechanization. Although puddled transplanted rice has been more widely used in the past, direct seeding has been gaining popularity in recent years, especially due to its water- and labor-saving features. This technique allows full crop establishment from seeds that were directly sown in the field, thus avoiding puddling, transplanting, and maintaining standing water. Consequently, it offers promising positive environmental effects including decreasing the release of greenhouse gases and increasing water-use efficiency. Historically, rice varieties bred for transplanting are also used in direct seeding, which limits the maximum yield potential of field trials. The success of direct seeding relies strongly on the development of rice varieties with robust crop establishment. Anaerobic germination, seed longevity, and early seedling vigor are the key traits required to achieve this. This chapter expounds on the physiology, molecular mechanisms, genetics, and relevance of the enumerated traits for direct seeding. A brief discussion of breeding for rice varieties with improved germination under direct seeding is also provided.
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Roberts, E. H., and K. Osei-Bonsu. "Seed and seedling vigour." In World crops: Cool season food legumes. Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-2764-3_71.
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Lantican, Gaudencia A. "Field Screening of Gamma-Irradiated Cavendish Bananas." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana. Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_7.
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AbstractIn our search for Cavendish bananas to withstand Fusarium oxysporum f. sp. cubense (Foc TR4) and other diseases, field screening of tissue-cultured Grand Nain banana seedlings derived from gamma-irradiated shoot tips was explored. Six months after irradiation and multiplication in the laboratory, the plantlets (M1V6) were individually grown in seedling bags under screen house conditions for 8 weeks, side-by-side with non-irradiated plantlets of the same clone. Once acclimatized, the banana plants were grown in an area confirmed positive of Foc TR4 (based on previous farm records stating that more than 50% of the plant population succumbed to the disease). Seedlings from each treatment (dose of radiation) were divided into four replicates, regardless of the number of plants. Each plant was given a unique identification code for traceability during disease monitoring, bunch and fruit quality evaluation.Incidences of Foc TR4, Moko disease (Ralstonia solanacearum) and virus diseases were monitored weekly. Plants found positive of any disease were eradicated immediately. The plant population for the succeeding generation was managed by removing the unwanted suckers, 12 weeks from planting using a spade gouge and keeping only one sucker per plant for the next generation. Agronomic characters of each plant were taken at the flowering stage. These included age to flower, height, pseudostem circumference, number of leaves and height of the sucker. The bunch was harvested 12 weeks from flowering. The number of hands in a bunch, the number of fingers and weight of a hand were recorded. The same agronomic characters of the plant were taken for the succeeding generations.Plants left standing in the field without any disease symptoms 3 years after planting were considered as putative mutants and were selected as candidate lines for multiplication and second-generation field screening. Only healthy suckers (free from viruses) were further multiplied via tissue culture technique to reach M1V6. Clean suckers from each line free of soil debris or dirt were sent to the laboratory for multiplication. At least 1000 plantlets were produced from each line for the second-generation field screening. These were grown in two locations – with and without records of Foc TR4. Field monitoring activities including plant population management, disease incidence assessment and fruit quality evaluation were carried out following the same protocols used in the establishment of the first-generation plants. Lines with population showing ≤10% Foc TR4 after the first harvest, with good vigor, fruit quality and productivity were considered as candidates for further multiplication, farmers distribution and field planting under semi-commercial scale.
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Rao, Muhammad Junaid, Sajjad Hussain, Muhammad Akbar Anjum, et al. "Effect of Seed Priming on Seed Dormancy and Vigor." In Priming and Pretreatment of Seeds and Seedlings. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-8625-1_6.
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Basu, Sudipta, and Steven P. C. Groot. "Seed Vigour and Invigoration." In Seed Science and Technology. Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-5888-5_4.
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AbstractSeed vigour is an important aspect of seed quality. It is a quantitative trait which is responsible for overall seed performance in terms of rate and uniformity of seed germination, seedling growth, emergence ability under unfavourable environments and post storage performance. Seed vigour is controlled by genetic factors, initial seed quality, production environments, harvesting and storage conditions. Seed vigour tests provide a more sensitive index of seed performance per se than the germination test. Efforts have been focused on developing novel or improving existing methods of vigour estimation in different crops. The vigour tests are tools routinely used for in-house seed quality control programs, especially for field and vegetable crops. Some treatments can improve seed vigour, although the treatment effects are more evident under sub-optimum than optimum growing conditions. This chapter deals with different aspects of seed vigour and its effects on plant growth and discusses physiological and biochemical parameters to understand underlying mechanisms.
Conference papers on the topic "Seedling vigor"
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Zhang, Ling, Zhengxin Wang, Yadong Wang, Yulei Zhu, and Youhong Song. "Phenotyping the Seedling Vigor in Winter Wheat Based on Multispectral Images." In 2024 China Automation Congress (CAC). IEEE, 2024. https://doi.org/10.1109/cac63892.2024.10865475.
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Ajinde, Ayotunde, Tolulope Ogunnusi, Olufemi Dayo-Olagbende, Funmilayo Adeniyi, and Oghenerobor Akpor. "Evaluating the Impact of Plant Growth-Promoting Rhizobacteria Inoculums and Metabolites on Germination and Vigor Index of Sesame Seedlings." In 2024 IEEE 5th International Conference on Electro-Computing Technologies for Humanity (NIGERCON). IEEE, 2024. https://doi.org/10.1109/nigercon62786.2024.10927294.
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Lindsey, Raymond S., and J. Mitchell McGrath. "Examining salt stress for improvement of seedling vigor." In American Society of Sugarbeet Technologist. ASSBT, 2011. http://dx.doi.org/10.5274/assbt.2011.99.
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Naegele, Rachel P., and J. Mitchell McGrath. "Seedling vigor in beta vulgaris: The artistry of germination." In American Society of Sugarbeet Technologist. ASSBT, 2009. http://dx.doi.org/10.5274/assbt.2009.31.
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Lupascu, Galina, and Svetlana Gavzer. "Variabilitatea şi heritabilitatea vigorii boabelor de grâu comun la interacţiunea cu Drechslera Sorokiniana." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.68.
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The article presents data on the effects of the interaction of common wheat with the fungus Drechslera sorokiniana on grain vigor. Its differentiated action on germination and seedling length (LP) was found. The vigor index (VI) depended more on LP (r = 0.90 *) than on the germination 278 level (r = 0.52 *, p≤0.05). The coefficient of heritability in the broad sense (h2) was 71.3% for LP and 60.1% – for VI. The association of h2 and genetic progress with high values reveals the pronounced contribution of additive genetic variance in the control of the vigor index, which offers increased opportunities in the selection of wheat plants resistant to this pathogen in restricted terms.
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McGrath, J. Mitchell, Vinay Hiremath, Paul Galewski, and Safa Elzohary. "TRANSCRIPTIONS OF SEEDLINGS GERMINATING IN WATER, HYDROGEN PEROXIDE, AND SALT FOR DISCOVERY OF SEEDLING VIGOR GENES AND BIOCHEMICAL PATHWAYS." In 37th Biennial Meeting of American Society of Sugarbeet Technologist. ASSBT, 2013. http://dx.doi.org/10.5274/assbt.2013.28.
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Milošević, Nebojša, Ivana Glišić, Milena Đorđević, Sanja Radičević, and Slađana Marić. "UTICAJ NOVIH VEGETATIVNIH PODLOGA NA BUJNOST, PRINOS I POMOLOŠKE OSOBINE SORTI ŠLJIVE „ČAČANSKA LEPOTICA“ I „JOJO“." In XXVII savetovanje o biotehnologiji. University of Kragujevac, Faculty of Agronomy, 2022. http://dx.doi.org/10.46793/sbt27.161m.
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Despite numerous deficiencies, the Myrobalan seedling is the most common plum rootstock in the Republic of Serbia. Although a large number of vegetative rootstocks exists worldvide, they are generally not available to producers in our country. In this work, we investigae the influence of four vegetative rootstocks: ʻWavitʼ, ʻWeivaʼ, ʻDoceraʼ and ʻDospinaʼ (last two are hypersensitive to Sharka virus) on tree vigour, yield and pomological properties of two plum cultivars ʻJojoʼ and ʻC ačanska Lepoticaʼ. All rootstocks differently affected examined properties. The largest tree vigour in both cultivars was caused by ‘Weiva’, while the smallest tree vigour of ‘Čačanska Lepotica’ was affected by ‘Docera’ and ‘Wavit’, as well as by ‘Dospina’ in ‘Jojo’. The highest yield was induced by ‘Weiva’, and smallest by ‘Docera’ and ‘Wavit’. Rootstocks ‘Weiva’ and ‘Wavit’ showed the largest influence on fruit weight while ‘Dospina’ and ‘Wavit’ affected morphometric properties the most. In terms of cultivars, ‘Jojo’ had largest tree vigour, yield, fruit weight and morphometric properties compared to ‘Čačanska Lepotica’.
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Abouelezz, Ahmed Helmy Hassan, and Talaat Ahmed. "The Efficacy of Two Household Cleaning and Disinfecting Agents on Lentil (Lens culinaris Medik) and Faba bean (Vicia faba) Seed Germination." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0023.
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A germination test of Vicia faba and Lens culinaris seeds under the effect of bleach and vinegar was conducted for seven days, and the observations were recorded daily. The completely randomized design (CRD) was used to examine the germination with three replicates at the lab conditions. Six germination parameters were measured, including germination percentage (GP), germination index (GI), mean germination time (MGT), mean germination rate (MGR), vigour index (VI), plus the fresh weight (FW) and dry weight (DW) of Vicia faba and Lens culinaris seeds. As a legume crop seeds model, the efficacy of four treatment levels from 0.005% to 0.5% of bleach and vinegar on the germination was tested. A chemical analysis was performed using the ion chromatography (IC) to evaluate the effect of chloride and acetate anions up-take on the seedling germination in addition to other essential nutrients. A significant inhibition in seedling growth was observed with increasing the treatment concentration. The maximum inhibition was recorded for both seeds at 0.5%, followed by 0.1% levels, while a positive effect was represented with the lower concentrations. The chemical analysis of the up-taking active ingredients was corroborated by the germination outputs.
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Vasilenko, Vladimir. "Red/Infrared Light from Light Emitting Diodes Stimulates Germination and Improves Vigour of Seedlings." In International Conference On Environmental Systems. SAE International, 2000. http://dx.doi.org/10.4271/2000-01-2424.
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Mihăilă, Victoria, L. Korlateanu, Lolita Melian, and Alina Ganea. "Determination of storage potential of genotypes from the melon collection (Cucumis melo l.) in the conditions of Ex situ conservation." In Scientific International Symposium “Advanced Biotechnologies - Achievements and Prospects” (VIth Edition). Institute of Genetics, Physiology and Plant Protection, 2022. http://dx.doi.org/10.53040/abap6.2022.105.
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The conservation of genetic resources in genetic banks is considered the most advanced form of conservation of plant genetic material. The storage of plant germplasm in seed form is the most practical and, at the same time, the most practiced method of ex situ conservation, allowing the conservation of a large number of samples in a relatively small space. Regardless of the storage conditions (more or less favorable) the seeds age, lose quality and vigor. Seed aging is the result of the accumulation of metabolic and structural damage that conditions a dis-ruption of normal functioning and decreases resistance to adverse environmental factors, even up to the loss of germination capacity. Keeping the viability of the seed is the primary objective in the successful realization of its conservation ex situ. New methods need to be applied to de-termine the quality and storage potential of seeds within a short time. In order to achieve this goal, the method of accelerated aging of seeds (AA) can be used, which involves their thermal treatment, increasing their humidity and keeping the seeds in ovens at temperatures much hi-gher than those for long-term preservation (45 or even 55°C) [3]. The application of the test of accelerated aging of seeds allows to monitor the genotypes in the plant collection, to record the viability of the seeds and to display the genotypes that show a long-term storage capacity in the genetic bank. This communication describes the manifestation of some morpho-physiological and biochemical parameters in the seeds and seedlings of some melon varieties (Cucumis melo L.) after application of the test of seed accelerated aging.
Reports on the topic "Seedling vigor"
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Tunning, Tyler. Biological seed treatments’ effects on organic corn hybrid seedling emergence in laboratory vigor tests and field trials. Iowa State University, 2022. http://dx.doi.org/10.31274/cc-20240624-301.
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Boyle, Maxwell, and Elizabeth Rico. Terrestrial vegetation monitoring at Fort Pulaski National Monument: 2019 data summary. National Park Service, 2021. http://dx.doi.org/10.36967/nrds-2288716.
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The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and monitoring is currently conducted at 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. 2019 marks the first year of conducting this monitoring effort on four SECN parks, including Fort Pulaski National Monument (FOPU). Twelve vegetation plots were established at Fort Pulaski National Monument in August. Data collected in each plot included species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches {in}]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Fort Pulaski National Monument in 2019. Data were stratified across two dominant broadly defined habitats within the park (Maritime Tidal Wetlands and Maritime Upland Forests and Shrublands). Noteworthy findings include: Sixty-six vascular plant taxa were observed across 12 vegetation plots, including six taxa not previously known from the park. Plots were located on both Cockspur and McQueen’s Island. The most frequently encountered species in each broadly defined habitat included: Maritime Tidal Wetlands: smooth cordgrass (Spartina alterniflora), perennial saltmarsh aster(Symphyotrichum enuifolium), and groundsel tree (Baccharis halimifolia) Maritime Upland Forests and Shrublands: yaupon (Ilex vomitoria), southern/eastern red cedar (Juniperus silicicola + virginiana), and cabbage palmetto (Sabal palmetto). Four non-native species identified as invasive by the Georgia Exotic Pest Plant Council (GA-EPPC 2018) were found during this monitoring effort. These species (and their overall frequency of occurrence within all plots) included: Japanese honeysuckle (Lonicera japonica; 17%), bahiagrass (Paspalum notatum; 8%), Vasey’s grass (Paspalum urvillei; 8%), and European common reed (Phragmites australis; 8%). Two rare plants tracked by the Georgia Department of Natural Resources (GADNR 2013) were found during this monitoring effort. These include Florida wild privet (Forestiera segregata) and Bosc’s bluet (Oldenlandia boscii). Southern/eastern red cedar and cabbage palmetto were the most dominant species within the tree stratum of the maritime Upland Forest and Shrubland habitat type. Species that dominated the sapling and seedling strata of this type included yaupon, cabbage palmetto, groundsel tree, and Carolina laurel cherry (Prunus caroliniana). The health status of sugarberry (Celtis laevigata)—a typical canopy species in maritime forests of the South Atlantic Coastal Plain--observed on park plots appeared to be in decline, with most stems experiencing elevated levels of dieback and low vigor. Over the past decade, this species has been experiencing unexplained high rates of dieback and mortality throughout its range in the Southeastern United States; current research is focusing on what may be causing these alarming die-off patterns. Duff and litter made up the majority of downed woody biomass (fuel loads) across FOPU vegetation plots.
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Boyle, Maxwell, and Elizabeth Rico. Terrestrial vegetation monitoring at Timucuan Ecological and Historic Preserve: 2019 data summary—Version 2.0. National Park Service, 2022. http://dx.doi.org/10.36967/nrds-2290196.
Full textAbstract:
The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and it is currently conducted on 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. 2019 marks the first year of conducting this monitoring effort on four SECN parks, including Timucuan Ecological and Historic Preserve (TIMU). A total of 23 vegetation plots were established in the park in May and June. Data collected in each plot include species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches (in)]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Timucuan Ecological and Historic Preserve in 2019. Data were stratified across three dominant broadly defined habitats within the park (Coastal Plain Nonalluvial Wetlands, Coastal Plain Open Uplands and Woodlands, and Maritime Upland Forests and Shrublands) and three land parcels (Cedar Point, Theodore Roosevelt, and Thomas Creek). Noteworthy findings include: A total of 157 vascular plant taxa (species or lower) were observed across 23 vegetation plots, including nine species not previously known from the park. Three plots were located in the footprint of the Yellow Bluff Fire, and were sampled only two weeks following the fire event. Muscadine (Muscadinia rotundifolia), cat greenbrier (Smilax glauca), water oak (Quercus nigra), and swamp tupelo (Nyssa biflora) were the most frequently encountered species in Coastal Plain Nonalluvial Wetland habitat; saw palmetto (Serenoa repens), slash pine (Pinus elliottii), and gallberry (Ilex glabra) were the most frequently encountered species in Coastal Plain Open Upland and Woodland habitat; and Darlington oak (Quercus hemisphaerica), Spanish moss (Tillandsia usenoides), and red bay (Persea borbonia) were the most frequently encountered species in Maritime Upland Forests and Shrublands. There were no exotic species of the Florida Exotic Pest Plant Council list of invasive plants (FLEPPC 2020) observed on any of these plots. Both red bay and swamp bay (Persea palustris) were largely absent from the tree stratum in these plots; however, they were present (occasionally in high abundance) in the seedling and sapling strata across all habitat types. Buckthorn bully (Sideroxylon lycioides)—listed as Endangered in the state of Florida by the Florida Department of Agriculture and Consumer Services (FDACS 2020)—was observed in three Maritime Upland Forest and Shrubland plots. The tree strata in each broadly defined habitat were dominated by the following species: Coastal Plain Nonalluvial Wetlands-loblolly bay (Gordonia lasianthus) Coastal Plain Open Uplands and Woodlands-longleaf pine (Pinus palustris) Maritime Upland Forests and Shrublands-oaks (Quercus sp.) Most stems within the tree strata exhibited healthy vigor and only moderate dieback across all habitat types. However, there was a large amount of standing dead trees in plots within Maritime Upland Forests and Shrublands. Downed woody biomass (fuel loads) were highest in the Cedar Point and Thomas Creek land parcels.
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Boyle, M., and Elizabeth Rico. Terrestrial vegetation monitoring at Fort Matanzas National Monument: 2019 data summary. National Park Service, 2022. http://dx.doi.org/10.36967/nrds-2293409.
Full textAbstract:
The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and it is currently conducted at 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. 2019 marks the first year of conducting this monitoring effort at four SECN parks, including Fort Matanzas National Monument (FOMA). Nine vegetation plots, located on Anastasia and Rattlesnake Islands, were established at Fort Matanzas National Monument in June. Data collected in each plot included species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches {in}]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Fort Matanzas National Monument in 2019. Data were stratified across two dominant broadly defined habitats within the park (Maritime Upland Forests/Shrublands and Maritime Open Uplands). Noteworthy findings include: Eighty-two vascular plant taxa (species or lower) were observed across nine vegetation plots, including eight species not previously documented within the park. The most frequently encountered species in each broadly defined habitat included: Maritime Upland Forests and Shrublands: saw palmetto (Serenoa repens), yaupon (Ilex vomitoria), southern/eastern red cedar (Juniperus silicicola + virginiana), American beautyberry (Callicarpa americana), and American burnweed (Erectites hieraciifolius). Maritime Open Uplands: sea oats (Uniola paniculata), earleaf greenbriar (Smilax auriculata), and dixie sandmat (Euphorbia bombensis). ne non-native species, Brazilian pepper (Schinus terebinthifolia), categorized as invasive by the Florida Exotic Pest Plant Council (FLEPPC 2019) was encountered in one Maritime Upland Forest and Shrubland plot during this monitoring effort. There were not any rare plants tracked by the Florida Department of Agriculture and Consumer Services (FDACS 2020) found during this monitoring effort. All plants located in these monitoring plots are fairly common throughout Florida, as well as across the Southeast Coast. Three species observed, however, are on the FDACS 2020 list of commercially exploited plants within the state. These include saw palmetto, cinnamon fern (Osmundastrum cinnamomeum), and coontie (Zamia integrifolia var. umbrosa). Southern/eastern red cedar and cabbage palmetto (Sabal palmetto) were the most dominant species within the tree stratum of the Maritime Upland Forest and Shrubland habitat type. Species that dominated the sapling and seedling strata of this type included yaupon and cabbage palmetto. More than 75% of the trees measured in the parks Maritime Upland Forest and Shrubland habitat type were alive and experiencing healthy vigor. Of the 22 trees that were dead, more than 50% of those were southern/eastern red cedar. Most of those individuals that were observed with moderate or severe decline and greater than 50% dieback were southern/eastern red cedars. Although red bay (Persea borbonia) was identified as one of the “principal understory tree” species within Fort Matanzas National Monument’s maritime forests in 2004 (Zomlefer et al. 2004), tree-sized individuals were rarely detected on plots during this monitoring effort. This may be in part due to the detection of laurel wilt disease within St. Johns County in 2006 (USDA 2021). Based on the low detection...
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Boyle, Maxwell, and Elizabeth Rico. Terrestrial vegetation monitoring at Timucuan Ecological and Historic Preserve: 2019 data summary. National Park Service, 2021. https://doi.org/10.36967/2286623.
Full textAbstract:
The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and it is currently conducted on 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. 2019 marks the first year of conducting this monitoring effort on four SECN parks, including Timucuan Ecological and Historic Preserve (TIMU). A total of 23 vegetation plots were established in the park in May and June. Data collected in each plot include species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches (in)]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Timucuan Ecological and Historic Preserve in 2019. Data were stratified across three dominant broadly defined habitats within the park (Coastal Plain Nonalluvial Wetlands, Coastal Plain Open Uplands and Woodlands, and Maritime Upland Forests and Shrublands) and three land parcels (Cedar Point, Theodore Roosevelt, and Thomas Creek). Noteworthy findings include: A total of 157 vascular plant taxa (species or lower) were observed across 23 vegetation plots, including nine species not previously known from the park. Three plots were located in the footprint of the Yellow Bluff Fire, and were sampled only two weeks following the fire event. Muscadine (Muscadinia rotundifolia), cat greenbrier (Smilax glauca), water oak (Quercus nigra), and swamp tupelo (Nyssa biflora) were the most frequently encountered species in Coastal Plain Nonalluvial Wetland habitat; saw palmetto (Serenoa repens), slash pine (Pinus elliottii), and gallberry (Ilex glabra) were the most frequently encountered species in Coastal Plain Open Upland and Woodland habitat; and Darlington oak (Quercus hemisphaerica), Spanish moss (Tillandsia usenoides), and red bay (Persea borbonia) were the most frequently encountered species in Maritime Upland Forests and Shrublands. There were no exotic species of the Florida Exotic Pest Plant Council list of invasive plants (FLEPPC 2020) observed on any of these plots. Both red bay and swamp bay (Persea palustris) were largely absent from the tree stratum in these plots; however, they were present (occasionally in high abundance) in the seedling and sapling strata across all habitat types. Buckthorn bully (Sideroxylon lycioides)—listed as Endangered in the state of Florida by the Florida Department of Agriculture and Consumer Services (FDACS 2020)—was observed in three Maritime Upland Forest and Shrubland plots. The tree strata in each broadly defined habitat were dominated by the following species: Coastal Plain Nonalluvial Wetlands-loblolly bay (Gordonia lasianthus) Coastal Plain Open Uplands and Woodlands-longleaf pine (Pinus palustris) Maritime Upland Forests and Shrublands-oaks (Quercus sp.) Most stems within the tree strata exhibited healthy vigor and only moderate dieback across all habitat types. However, there was a large amount of standing dead trees in plots within Maritime Upland Forests and Shrublands. Downed woody biomass (fuel loads) were highest in the Cedar Point and Thomas Creek land parcels.
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Boyle, Maxwell, and Elizabeth Rico. Terrestrial vegetation monitoring at Cape Hatteras National Seashore: 2019 data summary. National Park Service, 2022. http://dx.doi.org/10.36967/nrr-2290019.
Full textAbstract:
The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and monitoring is currently conducted at 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. The first year of conducting this monitoring effort at four SECN parks, including 52 plots on Cape Hatteras National Seashore (CAHA), was 2019. Twelve vegetation plots were established at Cape Hatteras NS in July and August. Data collected in each plot included species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches {in}]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Cape Hatteras National Seashore in 2019. Data were stratified across four dominant broadly defined habitats within the park (Maritime Tidal Wetlands, Maritime Nontidal Wetlands, Maritime Open Uplands, and Maritime Upland Forests and Shrublands) and four land parcels (Bodie Island, Buxton, Hatteras Island, and Ocracoke Island). Noteworthy findings include: A total of 265 vascular plant taxa (species or lower) were observed across 52 vegetation plots, including 13 species not previously documented within the park. The most frequently encountered species in each broadly defined habitat included: Maritime Tidal Wetlands: saltmeadow cordgrass Spartina patens), swallow-wort (Pattalias palustre), and marsh fimbry (Fimbristylis castanea) Maritime Nontidal Wetlands: common wax-myrtle (Morella cerifera), saltmeadow cordgrass, eastern poison ivy (Toxicodendron radicans var. radicans), and saw greenbriar (Smilax bona-nox) Maritime Open Uplands: sea oats (Uniola paniculata), dune camphorweed (Heterotheca subaxillaris), and seabeach evening-primrose (Oenothera humifusa) Maritime Upland Forests and Shrublands: : loblolly pine (Pinus taeda), southern/eastern red cedar (Juniperus silicicola + virginiana), common wax-myrtle, and live oak (Quercus virginiana). Five invasive species identified as either a Severe Threat (Rank 1) or Significant Threat (Rank 2) to native plants by the North Carolina Native Plant Society (Buchanan 2010) were found during this monitoring effort. These species (and their overall frequency of occurrence within all plots) included: alligatorweed (Alternanthera philoxeroides; 2%), Japanese honeysuckle (Lonicera japonica; 10%), Japanese stilt-grass (Microstegium vimineum; 2%), European common reed (Phragmites australis; 8%), and common chickweed (Stellaria media; 2%). Eighteen rare species tracked by the North Carolina Natural Heritage Program (Robinson 2018) were found during this monitoring effort, including two species—cypress panicgrass (Dichanthelium caerulescens) and Gulf Coast spikerush (Eleocharis cellulosa)—listed as State Endangered by the Plant Conservation Program of the North Carolina Department of Agriculture and Consumer Services (NCPCP 2010). Southern/eastern red cedar was a dominant species within the tree stratum of both Maritime Nontidal Wetland and Maritime Upland Forest and Shrubland habitat types. Other dominant tree species within CAHA forests included loblolly pine, live oak, and Darlington oak (Quercus hemisphaerica). One hundred percent of the live swamp bay (Persea palustris) trees measured in these plots were experiencing declining vigor and observed with symptoms like those caused by laurel wilt......less
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Boyle, M., and Elizabeth Rico. Terrestrial vegetation monitoring at Cumberland Island National Seashore: 2020 data summary. National Park Service, 2022. http://dx.doi.org/10.36967/2294287.
Full textAbstract:
The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and it is currently conducted at 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. 2020 marks the first year of conducting this monitoring effort at Cumberland Island National Seashore (CUIS). Fifty-six vegetation plots were established throughout the park from May through July. Data collected in each plot included species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches {in}]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Cumberland Island National Seashore in 2020. Data were stratified across three dominant broadly defined habitats within the park, including Coastal Plain Upland Open Woodlands, Maritime Open Upland Grasslands, and Maritime Upland Forests and Shrublands. Noteworthy findings include: 213 vascular plant taxa (species or lower) were observed across 56 vegetation plots, including 12 species not previously documented within the park. The most frequently encountered species in each broadly defined habitat included: Coastal Plain Upland Open Woodlands: longleaf + pond pine (Pinus palustris; P. serotina), redbay (Persea borbonia), saw palmetto (Serenoa repens), wax-myrtle (Morella cerifera), deerberry (Vaccinium stamineum), variable panicgrass (Dichanthelium commutatum), and hemlock rosette grass (Dichanthelium portoricense). Maritime Open Upland Grasslands: wax-myrtle, saw greenbrier (Smilax auriculata), sea oats (Uniola paniculata), and other forbs and graminoids. Maritime Upland Forests and Shrublands: live oak (Quercus virginiana), redbay, saw palmetto, muscadine (Muscadinia rotundifolia), and Spanish moss (Tillandsia usneoides) Two non-native species, Chinaberry (Melia azedarach) and bahiagrass (Paspalum notatum), categorized as invasive by the Georgia Exotic Pest Plant Council (GA-EPPC 2018) were encountered in four different Maritime Upland Forest and Shrubland plots during this monitoring effort. Six vascular plant species listed as rare and tracked by the Georgia Department of Natural Resources (GADNR 2022) were observed in these monitoring plots, including the state listed “Rare” Florida swampprivet (Forestiera segregata var. segregata) and sandywoods sedge (Carex dasycarpa) and the “Unusual” green fly orchid (Epidendrum conopseum). Longleaf and pond pine were the most dominant species within the tree stratum of Coastal Plain Upland Open Woodland habitat types; live oak was the most dominant species of Maritime Upland Forest and Shrubland types. Saw palmetto and rusty staggerbush (Lyonia ferruginea) dominated the sapling stratum within Maritime Upland Forest and Shrubland habitat types. Of the 20 tree-sized redbay trees measured during this monitoring effort only three were living and these were observed with severely declining vigor, indicating the prevalence and recent historical impact of laurel wilt disease (LWD) across the island’s maritime forest ecosystems. There was an unexpectedly low abundance of sweet grass (Muhlenbergia sericea) and saltmeadow cordgrass (Spartina patens) within interdune swale plots of Maritime Open Upland habitats on the island, which could be a result of grazing activity by feral horses. Live oak is the dominant tree-sized species across...
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Guy, Charles, Gozal Ben-Hayyim, Gloria Moore, Doron Holland, and Yuval Eshdat. Common Mechanisms of Response to the Stresses of High Salinity and Low Temperature and Genetic Mapping of Stress Tolerance Loci in Citrus. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613013.bard.
Full textAbstract:
The objectives that were outlined in our original proposal have largely been achieved or will be so by the end of the project in February 1995 with one exception; that of mapping cold tolerance loci based on the segregation of tolerance in the BC1 progeny population. Briefly, our goals were to 1) construct a densely populated linkage map of the citrus genome: 2) map loci important in cold and/or salt stress tolerance; and 3) characterize the expression of genes responsive to cold land salt stress. As can be seen by the preceding listing of accomplishments, our original objectives A and B have been realized, objective C has been partially tested, objective D has been completed, and work on objectives E and F will be completed by the end of 1995. Although we have yet to map any loci that contribute to an ability of citrus to maintain growth when irrigated with saline water, our very encouraging results from the 1993 experiment provides us with considerable hope that 1994's much more comprehensive and better controlled experiment will yield the desired results once the data has been fully analyzed. Part of our optimism derives from the findings that loci for growth are closely linked with loci associated with foliar Cl- and Na+ accumulation patterns under non-salinization conditions. In the 1994 experiment, if ion exclusion or sequestration traits are segregating in the population, the experimental design will permit their resolution. Our fortunes with respect to cold tolerance is another situation. In three attempts to quantitatively characterize cold tolerance as an LT50, the results have been too variable and the incremental differences between sensitive and tolerant too small to use for mapping. To adequately determine the LT50 requires many plants, many more than we have been able to generate in the time and space available by making cuttings from small greenhouse-grown stock plants. As it has turned out, with citrus, to prepare enough plants needed to be successful in this objective would have required extensive facilities for both growing and testing hardiness which simply were not available at University of Florida. The large populations necessary to overcome the variability we encountered was unanticipated and unforeseeable at the project's outset. In spite of the setbacks, this project, when it is finally complete will be exceedingly successful. Listing of Accomplishments During the funded interval we have accomplished the following objectives: Developed a reasonably high density linkage map for citrus - mapped the loci for two cold responsive genes that were cloned from Poncirus - mapped the loci for csa, the salt responsive gene for glutathione peroxidase, and ccr a circadian rhythm gene from citrus - identified loci that confer parental derived specific DNA methylation patterns in the Citrus X Poncirus cross - mapped 5 loci that determine shoot vigor - mapped 2 loci that influence leaf Na+ accumulation patterns under non-saline conditions in the BC1 population - mapped 3 loci that influence leaf Na+ accumulation paterns during salt sress - mapped 2 loci that control leaf Cl- accumulation patterns under non-saline conditions - mapped a locus that controls leaf Cl- accumulation patterns during salt stress Screened the BC1 population for growth reduction during salinization (controls and salinized), and cold tolerance - determined population variation for shoot/root ratio of Na+ and Cl- - determined levels for 12 inorganic nutrient elements in an effort to examine the influence of salinization on ion content with emphasis on foliar responses - collected data on ion distribution to reveal patterns of exclusion/sequestration/ accumulation - analyzed relationships between ion content and growth Characterization of gene expression in response to salt or cold stress - cloned the gene for the salt responsive protein csa, identified it as glutathione peroxidase, determined the potential target substrate from enzymatic studies - cloned two other genes responsive to salt stress, one for the citrus homologue of a Lea5, and the other for an "oleosin" like gene - cold regulated (cor) genes belonging to five hybridization classes were isolated from Poncirus, two belonged to the group 2 Lea superfamily of stress proteins, the others show no significant homology to other known sequences - the expression of csa during cold acclimation was examined, and the expression of some of the cor genes were examined in response to salt stress - the influence of salinization on cold tolerance has been examined with seedling populations - conducted protein blot studies for expression of cold stress proteins during salt stress and vice versa