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Journal articles on the topic 'Semen and fertility'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Burilo, Amina Bitta, and Isaac Pastory Kashoma. "Effects of Semen Dosage and Insemination Frequency on Fertility and Hatchability in Horasi Chicken Ecotype." European Journal of Agriculture and Food Sciences 5, no. 5 (2023): 24–29. http://dx.doi.org/10.24018/ejfood.2023.5.5.713.

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The objective of this study was to assess the effect of Horasi chicken ecotype semen dilution, insemination dosage, and frequency of insemination on spermatozoa fertility potentials (hatchability and fertility). Twenty adult hens and four cockerels were used in a 2×2×2 factorial experiment. The factors were semen type (undiluted versus diluted semen), insemination volume (0.5 versus 0.1 ml), and insemination frequency (once versus twice inseminations per week). Egg fertility and hatchability among semen type, semen volume and insemination frequency varied from 68.35 ± 2.10 to 82.20 ± 2.29, and

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de Mercado, Eduardo, Helena Nieto-Cristóbal, Adrián Martín-San Juan, María José Martinez-Alborcia, and Manuel Álvarez-Rodríguez. "Could Individual Variability in Resistance to Cryopreservation (“Freezability”) Serve as a Biomarker Reflecting Boar Fertility?" Animals 15, no. 15 (2025): 2180. https://doi.org/10.3390/ani15152180.

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Subfertile boars often go undetected until they cause significant reproductive losses. Current semen quality assessments are limited in their ability to predict fertility, highlighting the need for complementary biomarkers. This study explored whether semen freezability could serve as an indirect indicator of boar fertility. Eighteen boars were classified based on historical fertility records and semen freezability, assessed by post-thaw quality. Fresh and post-thaw semen samples were analyzed using the CASA system and fluorescence microscopy. High-fertility boars showed significantly better m

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Balusa, Pranay, Swathi Bommu, and Shanmugam Murugesan. "Effect of Betaine and Raffinose in Cryopreservation Medium on Fertility in Kadaknath Chicken." Cryoletters 43, no. 5 (2022): 283–88. http://dx.doi.org/10.54680/fr22510110212.

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BACKGROUND: Kadaknath is an important indigenous chicken with black pigmentation and cryopreserved semen reputably had low fertility. OBJECTIVE: The aim of this study was to evaluate the effects of betaine and raffinose in semen extenders on post thaw semen parameters and fertility. MATERIALS AND METHODS: Semen was cryopreserved in 4% dimethyl sulfoxide (DMSO) with betaine supplemented at 0.1, 0.2 and 0.4 M or raffinose supplemented at 1, 5 and 10 mM. Post thaw semen parameters and fertility were evaluated. RESULTS: Betaine at higher concentrations significantly (p < 0.05) inhibited the pos

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Foresta, Carlo, Marco Rossata, Alberto Ferlin, and Andrea Garolla. "Semen quality and fertility." Lancet 352, no. 9143 (1998): 1861–62. http://dx.doi.org/10.1016/s0140-6736(05)79933-5.

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5

Murugesan, Shanmugam, and Ramkrishna Mahapatra. "Effect of zinc supplementation during cryopreservation on post-thaw chicken semen parameters and fertility." Journal of the Hellenic Veterinary Medical Society 73, no. 1 (2022): 3841–44. http://dx.doi.org/10.12681/jhvms.25997.

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The present study evaluated supplementation of zinc from different sources during chicken semen cryopreservation on post-thaw semen quality and fertility. Adult White Leghorn chicken semen was cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). In the semen cryomixture zinc oxide (6.25 and 12.5 µM) and zinc sulphate (100 and 200 µM) was added and cryopreserved. The plastic semen straws were thawed at 5°C for 100 sec and samples were evaluated for sperm motility, live, abnormal and acrosome intact sperm. The lipid peroxidation in the seminal plasma was evaluated. The semen

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Murugesan, Shanmugam, and Ramkrishna Mahapatra. "Effect of zinc supplementation during cryopreservation on post-thaw chicken semen parameters and fertility." Journal of the Hellenic Veterinary Medical Society 73, no. 1 (2022): 3841–44. http://dx.doi.org/10.12681/jhvms.25997.

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The present study evaluated supplementation of zinc from different sources during chicken semen cryopreservation on post-thaw semen quality and fertility. Adult White Leghorn chicken semen was cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). In the semen cryomixture zinc oxide (6.25 and 12.5 µM) and zinc sulphate (100 and 200 µM) was added and cryopreserved. The plastic semen straws were thawed at 5°C for 100 sec and samples were evaluated for sperm motility, live, abnormal and acrosome intact sperm. The lipid peroxidation in the seminal plasma was evaluated. The semen

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7

de Andrade, André F. "175 Analysis of Cryopreserved Semen Quality: With the Tools Available, What Is Possible to Be Interpreted." Journal of Animal Science 99, Supplement_1 (2021): 112–13. http://dx.doi.org/10.1093/jas/skab054.185.

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Abstract Identifying doses of post-thawed semen with high and low fertility potential is the main objective pursued by professionals and companies involved in commercializing frozen semen. In post-thaw semen, we can evaluate the motility characteristics and the integrity of the spermatozoa membranes and their DNA so that we can identify the number of cells that have the minimum characteristics to be considered with the potential to fertilize the oocyte. However, even with these analyzes using fluorescent probes, flow cytometry, and a computerized semen analysis system, it is impossible to pred

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ZINAMAN, MICHAEL J., CHARLES C. BROWN, SHERRY G. SELEVAN, and ERIC D. CLEGG. "Semen Quality and Human Fertility: A Prospective Study With Healthy Couples." Journal of Andrology 21, no. 1 (2000): 145–53. http://dx.doi.org/10.1002/j.1939-4640.2000.tb03284.x.

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Abstract: Measures of semen quality are used as surrogate measures of male fertility in clinical andrology, reproductive toxicology, epidemiology, and risk assessment. However, only limited data are available to relate those measures to fertility. This prospective study with 210 reproductive‐age couples was conducted to provide information on the value of semen quality measures for predicting human male fertility potential and for development of models to estimate the effects of changes in semen quality on fertility in a given population for risk assessment. Couples without known risk factors

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Pimpa, Jutarat, Supakorn Authaida, Wuttigrai Boonkum, Sarinya Rerkyusuke, Chalinee Janta, and Vibuntita Chankitisakul. "Unveiling the Potential of Aloe vera Gel Supplementation in a Cooling Extender: A Breakthrough in Enhancing Rooster Sperm Quality and Fertility Ability." Animals 14, no. 16 (2024): 2290. http://dx.doi.org/10.3390/ani14162290.

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The cooling of semen storage at 5 °C from a Thai native rooster (Pradu Hang Dum), supplemented with herbs possessing antioxidant properties, provided limited research. This study was conducted to evaluate the efficiency of Aloe vera (AV) gel supplementation at various levels on the quality of cooled semen and subsequent fertility after artificial insemination. Sixty-four chickens had semen pooled, diluted, and supplemented with different levels of AV gel (0% as control, 0.25%, 0.50%, 1.0%, 2.5%, 5.0%, 10%, and 20%), and then stored for 72 h. In Experiment 1, semen quality, malondialdehyde (MDA

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Bintari, Intan Galuh, Iswati Iswati, Setya B. Udrayana Udrayana, and Abu Bakar. "Fertility and hatchability of KUB chicken eggs results from artificial insemination using liquid semen with carrot juice supplementation." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 14, no. 4 (2024): 170–74. https://doi.org/10.46549/jipvet.v14i4.475.

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Fertility is the success of spermatozoa in fertilizing an egg cell, while hatchability is a number that shows an egg's high or low ability to hatch. This research aims to determine the fertility and hatchability of KUB chicken eggs that have been stored cold by adding carrot juice to the basic diluent Ringer Lactate Egg Yolk. There were 60 KUB chickens divided into two treatment groups, each comprising 30 KUB hens. The group of eggs resulting from artificial insemination used fresh semen with Ringer Lactate diluent (P0), and eggs resulting from artificial insemination used liquid semen, which

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Waters, Tarah H. B., Carol Pui Shan Chan, Tin Chiu Li, and David Yiu Leung Chan. "Influence of the Period of Abstinence on Semen Quality in a Patient with Systemic Lupus Erythematosus: A Case Report and Review of the Literature." Case Reports in Rheumatology 2020 (March 9, 2020): 1–6. http://dx.doi.org/10.1155/2020/9296858.

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Systemic lupus erythematosus (SLE) is a chronic inflammatory disease that can affect fertility. There is currently little information regarding the semen profile of males with SLE. Moreover, there is no consensus on an appropriate period of sexual abstinence for semen analysis and on the use of DNA fragmentation assay, together with multiple semen analyses to document the semen profile in this clinical population. In this case report, multiple semen analyses, with DNA fragmentation assays, were performed for a male with SLE undergoing fertility treatment at our andrology unit. A 4-day period o

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Muthukumar, Shruthivishali, and Prathima Tholeti. "Semen Cryopreservation in Oncofertility." Fertility Science and Research 11 (May 10, 2024): 4. http://dx.doi.org/10.25259/fsr_46_23.

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Oncofertility, an interdisciplinary field, aims at improving the uality of life of cancer patients prone to iatrogenic infertility by offering fertility preservation options for a potentially fertile future. Semen cryopreservation (SC) is a well-established and effective way of preserving fertility in adolescent and young adult cancer-diagnosed males as they face potential fertility loss due to cancer and its treatments. The pathophysiology of certain cancers, such as testicular cancer or Hodgkin’s lymphoma, has been shown to have an adverse effect on semen uality. Ablative therapies like chem

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S.M. Thiab, R., and T. J. Mahdi. "THE COMPARISON OF FERTILIZING CAPACITY OF NATIVE AND NEW HAMPSHIRE MALE CHICKENS." Iraqi Journal of Veterinary Medicine 11, no. 1 (1987): 197–206. http://dx.doi.org/10.30539/1e5qnh52.

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This experiment was conducted to compare and estimate the fertilizing capacity of native with New Hampshire chicken semen. Also to study the effect. Of different levels of semen dosage on fertility percentage, duration’ of fertility and duration of fertilizing ability. In this experiment (12) native and (12) N.H. male chicken with (240) Hisex laying hens have been used. The results of the statistical analysis showed that there were no significant (P>0.05) differences between the native and N.H. male chickens for the fertilizing capacity. fertilizing percentage in duration of fertility and f

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Fatmila, Dian Tria, Yudi, Bambang Purwantara, Fuad Hasan, and Wahyudi Himawan Sutanto. "Display of fresh semen quality, frozen semen production, and field fertility of Bali bulls (Bos sondaicus) in different season." Indonesian Journal of Agricultural Research 8, no. 2 (2025): 47–57. https://doi.org/10.32734/injar.v8i2.20096.

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Bali cattle is one of Indonesia's local cattle that has high economic potential. The quality of the semen used plays an important role as a factor in the success of artificial insemination (AI) programs. Climate change, including temperature variations, rainfall, and length of solar exposure, can affect the quality of fresh semen produced by bulls. Differences in environmental conditions can affect semen characteristics, such as spermatozoa concentration, motility, and morphology, which in turn have an impact on the field fertility rate. The aim of this study is to explore the differences in m

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Fadl, A. M. "Effect of seasonality on quality and fertility of cryopreserved New Zealand white rabbit semen under Egyptian conditions." World Rabbit Science 28, no. 3 (2020): 123. http://dx.doi.org/10.4995/wrs.2020.13627.

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<p>The present study was designed to investigate the effect of seasonality on the quality and fertility of cryopreserved New Zealand white (NZW) rabbit semen under Egyptian conditions. Semen was collected from fifteen mature rabbit bucks during winter, spring, summer and autumn seasons, diluted in INRA-82 extender and cryopreserved in liquid nitrogen. Following the freezing/thawing process, semen samples were evaluated for semen quality parameters. We also investigated whether seasonality could affect postthaw fertility. The results showed that semen collected and processed during winter

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16

Damke, Edilson, Fábio A. Kurscheidt, Mary M. T. Irie, Fabrícia Gimenes, and Marcia E. L. Consolaro. "Male Partners of Infertile Couples With Seminal Positivity for Markers of Bacterial Vaginosis Have Impaired Fertility." American Journal of Men's Health 12, no. 6 (2018): 2104–15. http://dx.doi.org/10.1177/1557988318794522.

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To access the possibility that key markers of bacterial vaginosis (KM-BV) could affect seminal parameters and thus fertility a prospective cohort study was designed (a) to develop rapid and sensitive multiplex polymerase chain reaction (M-PCR) assays to screen 13 key markers of bacterial vaginosis (KM-BV) in semen specimens, (b) to determine the prevalence of KM-BV in semen from randomized male partners of couples seeking fertility evaluation. A total of 229 semen samples were included in the study from males who visited the Sperm Analysis Section of Brazil between October 2015 and March 2016.

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17

Umesiobi, D. O., M. U. lloeje, and N. A. Berepubo. "Artificial insemination in sows using Guelph and two local semen extenders." Nigerian Journal of Animal Production 29, no. 1 (2021): 121–26. http://dx.doi.org/10.51791/njap.v29i1.1541.

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Sixty two and half-year-old Large White sows and 12 boars of the same breed and age were used to study the comparative effects of conventional (Guelph) and local (coconut water and fresh raphia palm wine) extenders and storage (3 days) on the fertility of artificially inseminated sows. Semen was collected twice a week by gloved-hand method. Semen was either extended or unextended and evaluated on the day of collection (day 0) and during day 3 of storage for percentage sperm motility, acrosome morphology and fertility (Non-return rate, farrowing rate and litter size). Both Guelph and local exte

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18

Greenfield, Robert, Brian Birch, Alan Cooper, and Bashir Lwaleed. "Does human semen contain a functional haemostatic system?" Thrombosis and Haemostasis 93, no. 05 (2005): 847–52. http://dx.doi.org/10.1160/th04-09-0600.

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SummaryThere is already evidence that a few components of the haemostatic system exist in semen. If these comprise a functional system, they may have a role in seminal clotting and liquefaction processes and ultimately may influence fertility. What might be expected in semen as collected from fertility clinics i.e., after having both coagulated and subsequently liquefied is uncertain. It does however still contain significant amounts of Tissue Factor (TF) although its effect on semen quality remains poorly understood. The present study analyses semen for Tissue Factor Pathway Inhibitor (TFPI).

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Reed, E., W. G. Sanger, and J. O. Armitage. "Results of semen cryopreservation in young men with testicular carcinoma and lymphoma." Journal of Clinical Oncology 4, no. 4 (1986): 537–39. http://dx.doi.org/10.1200/jco.1986.4.4.537.

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Sixty-two young men with testicular carcinoma (31 patients) or lymphoma (31 patients) were referred for semen analysis and possible cryopreservation before systemic therapy. Seventy-seven percent of the patients, 24 patients with testicular carcinoma and 24 patients with lymphoma, had semen with a decreased chance for fertility (ie, sperm density less than 20 X 10(6)/mL and/or less than 40% motile sperm and/or decreased sperm motility). A decreased number of motile sperm as well as a poor grade of motility appeared in the majority of semen specimens from both patient groups. However, 14 patien

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Bailey, J., A. Morrier, and N. Cormier. "Semen cryopreservation: Successes and persistent problems in farm species." Canadian Journal of Animal Science 83, no. 3 (2003): 393–401. http://dx.doi.org/10.4141/a03-024.

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Artificial insemination has arguably been the most important practice contributing to the advancement of animal production. The numerous advantages of artificial insemination are augmented when the semen is cryopreserved and conserved for long periods. Unfortunately, the utility of cryopreserved semen is limited because for most mammals, even cattle, a considerable proportion of sperm loose their fertility during freezing-thawing. In many species , this loss of fertility is substantial, rendering cryopreserved semen impractical for routine use. Our goals are to characterize the nature of the s

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Pardede, Berlin Pandapotan, Muhammad Agil, Yudi Yudi, and Iman Supriatna. "Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program." Veterinary World 13, no. 12 (2020): 2649–57. http://dx.doi.org/10.14202/vetworld.2020.2649-2657.

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Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen

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Wambeke, F. Van, and N. Fujihara. "The effect of transparent fluid on fertility, hatchability and embryonic mortality following insemination of a normal number of stored fowl spermatozoa." Canadian Journal of Animal Science 74, no. 3 (1994): 475–78. http://dx.doi.org/10.4141/cjas94-067.

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Pure semen and semen containing 30% transparent fluid (TF) were diluted twofold in Van Wambeke's diluent and stored for 24 h at 2–5 °C. Caged dwarf breeders were inseminated weekly for 5 consecutive weeks with: (a) 0.08 mL of pure, stored semen per hen (310 × 106 sp.), (b) 0.08 mL of semen containing TF (217 × 106 spermatozoa (sp.)), (c) 0.092 mL of semen containing TF (250 × 106 sp.), or (d) 0.025 mL of pure, fresh semen (194 × 106 sp.). There were no significant differences (P > 0.05) in fertility, hatchability and embryonic mortality between the fresh control and the pure, stored semen n

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F. G. AL-Gebouri and S. M. Eidan. "METABOLITES AND SEMEN CHARACTERISTICS IN DIFFERENT BULLS FERTILITY." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 55, Special (2024): 206–16. http://dx.doi.org/10.36103/ijas.v55ispecial.1899.

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This study was investigated the relationship between Holstein bulls' fertility with some biomarkers (amino، fatty، and organic acids) and semen characteristics. The study included exploring the relationship among the fertility of Holstein bulls، several biomarkers، and semen characteristics in straws (20-25 straws/bull) preserved for three years. The cows were artificially inseminated (9-10 cows/bull) from semen. Based on the fertility rate، the bulls were divided into three groups، the first bulls of medium fertility (˂65%)، the second of good fertility (65-79%)، and the third of high fertili

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Costes, Valentin, Eli Sellem, Sylvain Marthey, et al. "Multi-omics data integration for the identification of biomarkers for bull fertility." PLOS ONE 19, no. 2 (2024): e0298623. http://dx.doi.org/10.1371/journal.pone.0298623.

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Bull fertility is an important economic trait, and the use of subfertile semen for artificial insemination decreases the global efficiency of the breeding sector. Although the analysis of semen functional parameters can help to identify infertile bulls, no tools are currently available to enable precise predictions and prevent the commercialization of subfertile semen. Because male fertility is a multifactorial phenotype that is dependent on genetic, epigenetic, physiological and environmental factors, we hypothesized that an integrative analysis might help to refine our knowledge and understa

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Serkis, Barbara, Zuzanna Bentkowska, Małgorzata Miazga, et al. "The Impact of Diet on Male Fertility." Quality in Sport 17 (July 21, 2024): 53057. http://dx.doi.org/10.12775/qs.2024.17.53057.

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Introduction: Infertility is a reproductive health issue where the inability to conceive after 12 months of regular unprotected intercourse affects about 8 to 12% of couples of reproductive age. Factors such as diet, lifestyle, and exposure to toxins can negatively impact men's reproductive capacity, including semen parameters and gonadal function. The impact of diet on male fertility, including hormone levels and the process of spermatogenesis, is the subject of intense research. Aim of the Study: This study aims to review current scientific research on the impact of diet on male fertility to

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SÁNCHEZ‐PARTIDA, L. GABRIEL, DAVID P. WINDSOR, JEFF EPPLESTON, BRIAN P. SETCHELL, and W. M. CHISHOLM MAXWELL. "Fertility and Its Relationship to Motility Characteristics of Spermatozoa in Ewes After Cervical, Transcervical, and Intrauterine Insemination With Frozen‐Thawed Ram Semen." Journal of Andrology 20, no. 2 (1999): 280–88. http://dx.doi.org/10.1002/j.1939-4640.1999.tb02519.x.

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ABSTRACT: The fertility of ewes after artificial insemination and the relationship between fertility and motility characteristics assessed by a computerized motility analysis system were examined with ram semen frozen in diluents reported to improve postthaw motility. The percentages of motile and progressive spermatozoa were better when frozen in proline—or glycine betaine‐containing or HEPES‐based, rather than Trisbased, diluents (P < 0.01). The fertility of spermatozoa frozen in diluents containing proline or glycine betaine was slightly reduced, whereas when both compatible solutes were

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Engelsman, Michelle, Leisa-Maree L. Toms, Xianyu Wang, Andrew P. W. Banks, and Debbie Blake. "Effects of firefighting on semen parameters: an exploratory study." Reproduction and Fertility 2, no. 1 (2021): L13—L15. http://dx.doi.org/10.1530/raf-20-0070.

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Lay summary Firefighters are occupationally exposed to heat intensities and chemical concentrations that may affect fertility. Twenty firefighters participated in an exploratory study assessing fertility of firefighters via an online survey and semen analysis. Data analysis included consideration of demographic characteristics, reproductive history and occupational exposures. Overall, firefighter semen parameters were below World Health Organisation reference values designating fertility in men. Firefighters younger than 45 years had a higher incidence of abnormal semen parameters (42%) than t

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Talwar, Pankaj. "Semen Banking and Cryobiology." International Journal of Infertility & Fetal Medicine 2, no. 2 (2011): 51–60. http://dx.doi.org/10.5005/jp-journals-10016-1018.

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ABSTRACT Cryo banking of spermatozoa is an essential aspect of fertility preservation. With the advancements in cryobiology and better understanding of cryoprotectants and assisted reproduction, indications for semen banking are expanding. The exponential developments that have occurred over the years in the field of cryopreservation have proved that frozen sperm is as good as fresh sperm in fertilizing oocytes. Semen banking has major role in fertility preservation in cancer patients.

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Islam, MM, AS Apu, SAM Hoque, MY Ali, and S. Karmaker. "Comparative study on the libido, semen quality and fertility of Brahman cross, Holstein Friesian cross and Red Chittagong breeding bulls." Bangladesh Journal of Animal Science 47, no. 2 (2018): 61–67. http://dx.doi.org/10.3329/bjas.v47i2.40236.

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The present study was undertaken to compare the performance of 8 (eight) breeding bulls of three different genetic groups such as Brahman × local (4 bulls), Holstein Friesian × local (2 bulls) and Red Chittagong (2 bulls) based on libido, semen quality and fertility. Semen parameters were evaluated from 30 ejaculates from each bull and fertility rate was calculated based on 60-days non-return rate via AI using these semen samples. Genetic group of breeding bulls had significant (p<0.05) effect on libido. Holstein Friesian cross breeding bulls showed significantly (p<0.05) highest libido

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Tucker, L. A., T. Matson, and L. H. A. Morris. "Variability in semen quality from British sport horses." Proceedings of the British Society of Animal Science 2007 (April 2007): 29. http://dx.doi.org/10.1017/s1752756200019323.

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Recent advances in artificial insemination (AI) and semen preparation have led to an increase in the availability of equine genetics globally. Generally, less than half the stallion population produce semen of suitable robustness to maintain acceptable fertility post-freezing, and research is needed to improve not only the numbers of stallions whose semen can be successfully frozen, but also to increase the number of collections that meet fertility standards. The number of progressively motile sperm present during ovulation determines successful conception in horses. AI practitioners recommend

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IBRAHIM, NASRELDIN M., GLEN R. GILBERT, KATHRYN J. LOSETH, and BO G. CRABO. "Correlation Between Clusterin‐Positive Spermatozoa Determined by Flow Cytometry in Bull Semen and Fertility." Journal of Andrology 21, no. 6 (2000): 887–94. http://dx.doi.org/10.1002/j.1939-4640.2000.tb03419.x.

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ABSTRACT: The objectives were to 1) develop a rapid and accurate method for detection of clusterin‐positive spermatozoa (CPS) in bull semen and 2) determine the utility of incidence of CPS for prediction of fertility of bull semen in comparison to routine semen quality traits. Semen from 3 bulls was immunostained with anti‐bovine clusterin antibody and with FITC‐conjugated anti‐rabbit IgG for method development. Clusterin‐positive spermatozoa were determined by flow cytometry (FCM) and fluorescence microscopy, and results were compared by paired t test. There was no difference between FCM and

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Getachew, Tarekegn, Gebeyehu Goshu, and Alemayehu Lemma. "Effects of Commercial and Homemade Extenders on Post-thaw Sperm Quality and Fertility of Semen from Ethiopian Indigenous Horro Chicken Breed." World's Veterinary Journal 13, no. 2 (2023): 341–47. http://dx.doi.org/10.54203/scil.2023.wvj37.

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Cryopreservation of spermatozoa represents an important strategy for in vitro programs designed for the conservation of the genetic material of livestock populations. The objective of this study was to evaluate the effects of homemade tris-egg yolk-based and commercial poultry semen extenders on post-thaw sperm quality, fertility, and hatchability of semen from the Ethiopian Indigenous Horro chicken breed. A total of 30 roosters were used for semen collection, and 160 adult hens were inseminated artificially. The collected, qualified, and pooled semen samples were divided into three groups. Ea

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Carneiro, Marcos Coura, Alice Caroline Souza e. Castro, Roberta Reis Silva, et al. "Comparison of Post-Thaw Motility and In Vitro Fertility Between Ejaculated and Epididymal Semen, and Seminal cfDNA Characterization in Pantaneiro Bulls." Biology 14, no. 5 (2025): 465. https://doi.org/10.3390/biology14050465.

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This study evaluated the post-thaw motility and in vitro fertility of ejaculated and epididymal semen from Pantaneiro bulls and characterized cell-free DNA (cfDNA) in fresh seminal plasma. Semen from five bulls was collected via electroejaculation or post-mortem epididymal extraction. Fresh semen parameters and cfDNA concentrations were assessed before cryopreservation. Post-thaw sperm kinetics were evaluated using CASA at 0 and 6 h of incubation, and in vitro embryo development was analyzed following IVF. Data were assessed using ANOVA and logistic regression. Ejaculate samples exhibited more

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Kim, S. W., C. Kim, I. S. Jeon, and Y. G. Ko. "35 The effect of fatty acid-free BSA supplementation on the ability of low-temperature-preserved chicken semen." Reproduction, Fertility and Development 31, no. 1 (2019): 143. http://dx.doi.org/10.1071/rdv31n1ab35.

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The dilution of chicken semen is one of the important processes for low-temperature storage and cryopreservation and is considered to be a highly applicable method for semen exchanges between small farms in developing countries. However, studies on chicken semen preservation have been limited for several reasons. First, dilution shocks chicken semen, with more than 4 to 6 times deterioration of sperm qualities. For example, approximately 8 to 10 times dilution reduces the activity and vitality of chicken semen. The dilution factor of chicken semen could not compete with that of mammals such as

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Khan, Haroon Latif, Yousaf Latif Khan, Irfan Mehfooz, Muhammad Burhan, Saba Sardar, and Abdul Rahman Khawaja. "SEMEN PARAMETERS." Professional Medical Journal 23, no. 05 (2016): 589–96. http://dx.doi.org/10.29309/tpmj/2016.23.05.1589.

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Semen is a pale whitish fluid secreted by male during ejaculation and containsspermatozoa which are male gametes essential of fertilizing the oocytes which are femalegametes. In a quest to evaluate male’s fertility potential semen is analyzed to look into some ofits characteristics and of the sperms contained within the semen analyzed. Method of collectioninfluences the results of Semen analysis as does the technique of analysis. Spermatozoa areexamined for number (count), shape (morphology) and movement (motility) in order to assesstheir quality. Non sperm cells, volume, Fructose level, pH, l

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Palmer, D. K., and S. N. M. AI-Maskari. "An Improved Diluent for Long-Term Liquid Storage of Chicken Semen." Journal of Agricultural and Marine Sciences [JAMS] 5, no. 2 (2000): 47. http://dx.doi.org/10.24200/jams.vol5iss2pp47-52.

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A prototype chicken semen diluent formulation has been developed based on the results of preliminary experiments. Preliminary experiments indicated the substitution of glutamate for the acetate and phosphate generally present in chicken semen diluents improved post-insemination fertility levels obtained from chicken semen following 24-96 hours of in vitro storage. Additional preliminary experiments indicated potassium could be substituted for the sodium generally contained in chicken semen diluents without affecting post-insemination fertility levels. The dual biological buffers, BES and BIS-T

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Pardede, Berlin Pandapotan, Muhammad Agil, Ni Wayan Kurniani Karja, Cece Sumantri, Iman Supriatna, and Bambang Purwantara. "PRM1 Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls." Veterinary Sciences 9, no. 3 (2022): 111. http://dx.doi.org/10.3390/vetsci9030111.

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Functional genes and proteins in sperm play an essential role in bulls’ reproductive processes. They are more accurate in determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for packaging and protecting sperm DNA until fertilization affects normal sperm function. This study analyzes the genes and proteins potential from PRM1 as fertility markers for different breeds of bulls utilized in the artificial insemination programs, expected to be an accurate tool in interpreting bull fertility in Indonesia. This study used Limousin, Holstei

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Rakha, Bushra Allah, Muhammad Sajjad Ansari, Shamim Akhter, and Elisabeth Blesbois. "Cryoprotective Effect of Glycerol Concentrations on Indian Red Jungle Fowl (Gallus Gallus Murghi) Spermatozoa." Avian Biology Research 11, no. 2 (2018): 80–88. http://dx.doi.org/10.3184/175815618x15180876264262.

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Semen cryopreservation protocols for wild avian species need to be optimised in order to achieve optimum post-thaw sperm quality and fertility. The present study was designed to evaluate the cryoprotective effect of different glycerol concentrations (11%, 15% and 20%) on post-thaw quality, recovery rates, absolute livability index and fertility of Indian Red Jungle Fowl (Gallus gallus murghi) semen. Semen was collected from eight mature cocks and cryopreserved for storage at −196 °C. Frozen semen was thawed at 37 °C for 30 s and assessed for motility, plasma membrane integrity, viability and a

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Leisegang, Kristian, Renata Finelli, Lionel Moungala, et al. "The Impact of COVID-19 Vaccines on Male Semen Parameters: A Retrospective Cohort Study." Andrologia 2023 (February 7, 2023): 1–9. http://dx.doi.org/10.1155/2023/7826568.

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The emergence of SARS-CoV-2 and the subsequent COVID-19 pandemic necessitated the development of adequate vaccines. Despite vaccines being demonstrated to be safe and effective for preventing severe disease and death, vaccine hesitancy remains. Reasons include concerns over adverse effects on male fertility, which have not been widely investigated. Therefore, this study is aimed at determining the impact of COVID-19 vaccination on semen parameters in a retrospective cohort study of South African males undergoing fertility assessment. The patients for this study were adult men who have previous

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Khalifa, Tarek, Constantinos Rekkas, Foteini Samartzi, Aristotelis Lymberopoulos, Kostas Kousenidis, and Toni Dovenski. "Highlights on Artificial Insemination (AI) Technology in the Pigs." Macedonian Veterinary Review 37, no. 1 (2014): 5–34. http://dx.doi.org/10.14432/j.macvetrev.2013.09.001.

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Abstract Over the past decade, there has been a tremendous increase in the development of field AI services in the majority of countries concerned with pig production. The objective of this paper is to review: (a) the current status of swine AI in the world, (b) significance and limitation of AI with liquid and frozen semen, (c) the biological traits of porcine semen in relation to in vitro sperm storage, (d) the criteria used for selection of a boar stud as a semen supplier, (e) how to process boar semen for liquid and frozen storage in the commercial settings and (f) how to improve fertility

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Talukdar, Dibya, Kutubuddin Ahmed, and Papori Talukdar. "Cryocapacitation and Fertility of Cryopreserved Semen." International Journal of Livestock Research 5, no. 6 (2015): 11. http://dx.doi.org/10.5455/ijlr.20150608041222.

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Graham, J. K., and E. Mocé. "Fertility evaluation of frozen/thawed semen." Theriogenology 64, no. 3 (2005): 492–504. http://dx.doi.org/10.1016/j.theriogenology.2005.05.006.

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Bonde, Jens Peter E., Erik Ernst, Tina Kold Jensen, et al. "Relation Between Semen Quality and Fertility." Obstetrical & Gynecological Survey 54, no. 4 (1999): 247–49. http://dx.doi.org/10.1097/00006254-199904000-00019.

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Papa, F. O., C. Ramires Neto, Y. F. R. Sancler-Silva, et al. "66 EFFECT OF ADDITION OF CHOLESTEROL-LOADED CYCLODEXTRIN BEFORE FREEZING ON QUALITY AND FERTILITY OF STALLION FROZEN SEMEN." Reproduction, Fertility and Development 27, no. 1 (2015): 126. http://dx.doi.org/10.1071/rdv27n1ab66.

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The aim of the present study was to evaluate the effect of cholesterol-loaded cyclodextrin on the quality and fertility of stallion frozen semen. Three ejaculates from each of 4 stallions were used. The semen was diluted (1 : 1) with a skim milk-based extender (Botu-SemenTM, Botupharma, Brazil). The samples were divided into 2 groups: control group (CG), composed of semen diluted only with extender, and treated group (TG), composed of semen diluted with extender plus 750 mg mL–1 of cholesterol-loaded cyclodextrin. Both groups were incubated for 15 min at 20°C. The semen was frozen with Botu-Cr

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Fadl, Aya Mohamed, Khaled Hafez El-Shahat, and Elshymaa Ahmed Abdelnaby. "Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen." World Rabbit Science 30, no. 3 (2022): 219–26. http://dx.doi.org/10.4995/wrs.2022.17042.

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Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabb

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Windsor, D. P. "Mitochondrial function and ram sperm fertility." Reproduction, Fertility and Development 9, no. 3 (1997): 279. http://dx.doi.org/10.1071/r96109.

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These experiments investigated the effect of freezing on mitochondrial function in ram sperm, the effectiveness of current freezing procedures in protecting mitochondria, and the role of mitochondrial respiration in cervical penetration and transit by ram sperm. Only sperm with functioning mitochondria (assessed by rhodamine 123 staining) after freezing and thawing were motile in a viscous medium (P < 0· 05). A simplified rhodamine 123 uptake assay was developed to monitor sperm mitochondrial function. The results of this procedure were highly correlated (r2 = 0 · 98) with the proportion of

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MS, Roshan Krishna, Babulal Natvarlal Suthar, Hareshbhai Chhotalal Nakhashi, and Karan Faljibhai Chaudhary. "Constraints Affecting Fertility of Sex Sorted Semen: An Overview." Indian Journal of Animal Reproduction 43, no. 1 (2022): 8–14. http://dx.doi.org/10.48165/ijar.2022.43.1.2.

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In most mammals, the male to female sex ratio of offspring is about 50% because half of the sperm contains either the Y chromosome or the X chromosome. Desired sex either male or female which is produced from semen having X or Y bearing sperm is known as sexed semen. Flow cytometry is the only proven method for semen sexing to be commercially viable with more than 90% accuracy to produce calves of desirable sex. The sexed semen technology has several advantages, the production of more female calves and more milk, and lowered risk of dystocia. Increased efficiency of progeny testing, embryo tra

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Chatzokou, Dimitra, Ermioni Tsarna, Efstathia Davouti, et al. "Semen Microbiome, Male Infertility, and Reproductive Health." International Journal of Molecular Sciences 26, no. 4 (2025): 1446. https://doi.org/10.3390/ijms26041446.

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The semen microbiome, once believed to be sterile, is now recognized as a dynamic ecosystem containing a diverse range of microorganisms with potential implications for male fertility and reproductive health. We aimed to examine the relationship between the semen microbiome, male infertility, and reproductive outcomes, highlighting the transformative role of next generation sequencing techniques and bioinformatics in exploring this intricate interaction, and we present a critical review of the published literature on this issue. Current evidence suggests a complex association between the compo

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SINGH, A. K., P. S. BRAR, and RANJNA S. CHEEMA. "Heparin binding proteins in seminal plasma of breeding buffalo bulls and their relation with semen freezability and in vivo fertility." Indian Journal of Animal Sciences 90, no. 3 (2020): 367–71. http://dx.doi.org/10.56093/ijans.v90i3.102424.

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The present study was conducted to determine the concentrations of heparin binding proteins (HBP) in seminal fluid of breeding buffalo bulls and establish their relationship with frozen-thawed semen characteristics and fertility. Both fresh and frozen semen were collected from same ejaculate of 30 breeding buffalo bulls. Based on HBP concentrations in seminal plasma obtained from affinity chromatography, all the bulls were divided into 2 groups, viz. high fertility bulls (HFB, ≥ 6.5 mg/ml; n=15) and low fertility bulls (LFB, < 6.5 mg/ml; n=15) for further comparisons. The frozen-thawed seme

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Naryshkina, Elena Nikolaevna, and Alexander Aleksandrovich Sermyagin. "The influence of the age of cows on the variability of the fertility estimate of the semen of Holstein bulls." Agrarian Scientific Journal, no. 12 (December 23, 2024): 127–32. https://doi.org/10.28983/asj.y2024i12pp127-132.

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The article considers the dynamics of variability of fertility of the semen of Holstein bulls (n=208) during insemination of heifers and cows of the 1st calving and older for the period 2015–2022 in the Moscow region. To obtain information on the fertilization of heifers and cows after a single insemination, an array of original information data characterizing their reproductive function. To assess the breeding value of bulls, the BLUP Sire Model was used, where the evaluation year (Yeari) and the age of the inseminated cow (Lactj) were used as fixed effects. Spearman's coefficient of rank rep

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