Academic literature on the topic 'Sequencing of 16S RNAr fragments'

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Journal articles on the topic "Sequencing of 16S RNAr fragments"

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Armani, A., L. Tinacci, X. Xiong, L. Castigliego, D. Gianfaldoni, and A. Guidi. "Fish species identification in canned pet food by BLAST and Forensically Informative Nucleotide Sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA)." Food Control 50 (April 2015): 821–30. http://dx.doi.org/10.1016/j.foodcont.2014.10.018.

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Zduńczyk, Zenon. "Functioning of the Intestinal Ecosystem: From New Technologies in Microbial Research to Practical Poultry Feeding – A Review." Annals of Animal Science 19, no. 2 (2019): 239–56. http://dx.doi.org/10.2478/aoas-2019-0007.

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AbstractUnlike classical microbiology which focuses on bacteria capable of growing in vitro, metagenomics is a study of genetic information originating from microflora which aims to characterise the microbiome, namely the common genome of bacteria, archaea, fungi, protozoa and viruses living in the host. Metagenomics relies on next-generation sequencing (NGS), a large-scale sequencing technique which allows millions of sequential reactions to be carried out in parallel to decode entire communities of microorganisms. Metagenomic analyses support taxonomic analyses (involving gene fragments enco
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Koizumi, Yoshikazu, John J. Kelly, Tatsunori Nakagawa, et al. "Parallel Characterization of Anaerobic Toluene- and Ethylbenzene-Degrading Microbial Consortia by PCR-Denaturing Gradient Gel Electrophoresis, RNA-DNA Membrane Hybridization, and DNA Microarray Technology." Applied and Environmental Microbiology 68, no. 7 (2002): 3215–25. http://dx.doi.org/10.1128/aem.68.7.3215-3225.2002.

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ABSTRACT A mesophilic toluene-degrading consortium (TDC) and an ethylbenzene-degrading consortium (EDC) were established under sulfate-reducing conditions. These consortia were first characterized by denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR-amplified 16S rRNA gene fragments, followed by sequencing. The sequences of the major bands (T-1 and E-2) belonging to TDC and EDC, respectively, were affiliated with the family Desulfobacteriaceae. Another major band from EDC (E-1) was related to an uncultured non-sulfate-reducing soil bacterium. Oligonucleotide probes specific
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Hori, Tomoyuki, Matthias Noll, Yasuo Igarashi, Michael W. Friedrich, and Ralf Conrad. "Identification of Acetate-Assimilating Microorganisms under Methanogenic Conditions in Anoxic Rice Field Soil by Comparative Stable Isotope Probing of RNA." Applied and Environmental Microbiology 73, no. 1 (2006): 101–9. http://dx.doi.org/10.1128/aem.01676-06.

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ABSTRACT Acetate is the most abundant intermediate of organic matter degradation in anoxic rice field soil and is converted to CH4 and/or CO2. Aceticlastic methanogens are the primary microorganisms dissimilating acetate in the absence of sulfate and reducible ferric iron. In contrast, very little is known about bacteria capable of assimilating acetate under methanogenic conditions. Here, we identified active acetate-assimilating microorganisms by using a combined approach of frequent label application at a low concentration and comparative RNA-stable isotope probing with 13C-labeled and unlab
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Janda, Ivan, and Karel Mikulík. "Preparation and sequencing of the cloacin fragment of Streptomycesaureofaciens 16S RNA." Biochemical and Biophysical Research Communications 137, no. 1 (1986): 80–86. http://dx.doi.org/10.1016/0006-291x(86)91178-2.

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Arakawa, Shizuka, Kohsuke Kamizaki, Yusuke Kuwana, et al. "Application of solid-phase DNA probe method with cleavage by deoxyribozyme for analysis of long non-coding RNAs." Journal of Biochemistry 168, no. 3 (2020): 273–83. http://dx.doi.org/10.1093/jb/mvaa048.

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Abstract The solid-phase DNA probe method is a well-established technique for tRNA purification. We have applied this method for purification and analysis of other non-coding RNAs. Three columns for purification of tRNAPhe, transfer-messenger RNA (tmRNA) and 16S rRNA from Thermus thermophilus were connected in tandem and purifications were performed. From each column, tRNAPhe, tmRNA and 16S rRNA could be purified in a single step. This is the first report of purification of native tmRNA from T. thermophilus and the purification demonstrates that the solid-phase DNA probe method is applicable t
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Schloss, Patrick D., Matthew L. Jenior, Charles C. Koumpouras, Sarah L. Westcott, and Sarah K. Highlander. "Sequencing 16S rRNA gene fragments using the PacBio SMRT DNA sequencing system." PeerJ 4 (March 28, 2016): e1869. http://dx.doi.org/10.7717/peerj.1869.

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Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina’s MiSeq, have allowed researchers to obtain millions of high quality but short sequences. The result of the added sequencing depth has been significant improvements in experimental design. The tradeoff has been the decline in the number of full-length reference sequences that are deposited into databases. To overcome this problem, we tested the ability of the
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Kozich, James J., Sarah L. Westcott, Nielson T. Baxter, Sarah K. Highlander, and Patrick D. Schloss. "Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform." Applied and Environmental Microbiology 79, no. 17 (2013): 5112–20. http://dx.doi.org/10.1128/aem.01043-13.

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ABSTRACTRapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone libraries to the sequencing of millions of fragments per study using next-generation sequencing technologies from 454 and Illumina. As these technologies advance, it is critical to assess the strengths, weaknesses, and overall suitability of these platforms for the interrogation of microbial communities. Here, we present an improved method for sequencing variable regions
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Callahan, Benjamin J., Joan Wong, Cheryl Heiner, et al. "High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution." Nucleic Acids Research 47, no. 18 (2019): e103-e103. http://dx.doi.org/10.1093/nar/gkz569.

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AbstractTargeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities. New long-read sequencing technologies can sequence the entire 16S rRNA gene, but higher error rates have limited their attractiveness when accuracy is important. Here we present a high-throughput amplicon sequencing methodology based on PacBio circular consensus sequencing and the DADA2 sample inference method that measures the full-length 16S rRNA gene with single-nucleotide resolution and a near-zero error rate. In two artificial
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Darwish, Ahmed M., and Adnan A. Ismaiel. "Genetic diversity of Flavobacterium columnare examined by restriction fragment length polymorphism and sequencing of the 16S ribosomal RNA gene and the 16S–23S rDNA spacer." Molecular and Cellular Probes 19, no. 4 (2005): 267–74. http://dx.doi.org/10.1016/j.mcp.2005.04.003.

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Dissertations / Theses on the topic "Sequencing of 16S RNAr fragments"

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Aguila, Nora Katia Saavedra del. "Avaliação de bactérias fototróficas em lagoas de estabilização: diversidade, purificação e identificação." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-22072008-175951/.

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As bactérias fototróficas freqüentemente apresentam florescimentos em lagoas de estabilização utilizadas no tratamento de esgoto sanitário, formando uma camada de cor púrpura na sua superfície. Portanto, o estudo das condições que propiciam tais florescimentos, a diversidade microbiana, o potencial de remoção da matéria orgânica e o estabelecimento das relações entre tais conhecimentos, permitem compreender o metabolismo do sistema. Nesse sentido, o objetivo deste trabalho foi avaliar a diversidade de bactérias (domínio Bacteria), bactérias fototróficas púrpuras e bactérias redutoras de sulfat
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Duarte, Iolanda Cristina Silveira. "Caracterização microbiológica da remoção e degradação de alquilbenzeno linear sulfonado (LAS) em reatores anaeróbios com biofilme e células planctônicas." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-13092006-162953/.

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O objetivo desse trabalho foi avaliar a degradação de alquilbenzeno linear sulfonado (LAS) em condições anaeróbias. Os primeiros experimentos foram realizados em reatores em batelada alimentados com diferentes substratos e concentrações de LAS. Apesar do surfactante ficar adsorvido no lodo, não foram observadas interferências no metabolismo de microrganismos anaeróbios, pois dessa forma o LAS tornou-se indisponível para a degradação celular. Reatores anaeróbios horizontais de leito fixo (RAHLF) foram avaliados quanto à remoção de LAS e inoculados com lodos anaeróbios provenientes de reatores U
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Lazaro, Carolina Zampol. "Obtenção e caracterização filogenética de consórcio de bactérias púrpuras não-sulforosas consumidoras de ácidos orgânicos visando a produção de hidrogênio em reator anaeróbio de batelada." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-16092009-090658/.

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O objetivo deste trabalho foi enriquecer consórcio microbiano a partir de mistura de lodo granular de digestor anaeróbio de fluxo ascendente sob condições fototróficas anoxigênicas. Por meio de técnica de biologia molecular foi possível identificar 17 unidades taxonômicas operacionais (UTO) no consórcio microbiano, dentre as quais seqüências similares a Rhodobacter, gênero amplamente citado nos estudos de produção de gás hidrogênio por bactérias fototróficas. Exames microscópicos do consórcio fototrófico indicaram predomínio de bacilos Gram-negativos. Ensaios sob condições fototróficas foram r
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Lopes, Patrícia Ferreira. "Diversidade taxonômica e potencial de biodegradação de bactérias isoladas de reservatórios de petróleo da Bacia de Campos (RJ)." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-22122010-095359/.

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O presente trabalho teve como objetivos caracterizar uma coleção de 98 bactérias isoladas de amostras de petróleo e água de formação de reservatórios da Bacia de Campos (RJ) utilizando técnicas de taxonomia molecular e avaliar o potencial de degradação de biomarcadores do petróleo. O sequenciamento e análise filogenética do gene RNAr 16S revelaram Bacillus firmus, megaterium, pumilus, sphaericus, simplex, cereus/B. thuringiensis Marinobacter lutaoensis, Halomonas shengliensis/H. alimentaria/ H.campisalis, Citreicella thiooxidans, Stenotrophomonas maltophilia, Achromobacter xylosoxidans, Microc
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Tran, Hoang-Dung [Verfasser]. "Sequencing fragments of cryptophyte plastomes from 16S rRNA to rbcL genes and phylogenetic analyses based on the protein-encoding genes located in these fragments / vorgelegt von Hoang-Dung Tran." 2009. http://d-nb.info/999683381/34.

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