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Relevant bibliographies by topics / Serial dilution

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Journal articles

Academic literature on the topic 'Serial dilution'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 June 2025

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Journal articles on the topic "Serial dilution"

1

Keler, Cynthia, Tabitha Balutis, Kim Bergen, Bryanna Laudenslager, and Deanna Rubino. "Serial Dilution Simulation Lab." American Biology Teacher 72, no. 5 (2010): 305–7. http://dx.doi.org/10.1525/abt.2010.72.5.9.

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Serial dilution is often a difficult concept for students to understand. In this short dry lab exercise, students perform serial dilutions using seed beads. This exercise helps students gain skill at performing dilutions without using reagents, bacterial cultures, or viral cultures, while being able to visualize the process.

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2

Arendrup, Maiken Cavling, Karin Meinike Jørgensen, Nicolien Hanemaaijer, and Paul E. Verweij. "ISO standard 20776-1 or serial 2-fold dilution for antifungal susceptibility plate preparation: that is the question!" Journal of Antimicrobial Chemotherapy 76, no. 7 (2021): 1793–99. http://dx.doi.org/10.1093/jac/dkab088.

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Abstract Background Since the ISO standard 20776-1 and serial dilution procedures were compared in 2010 for fluconazole and itraconazole, several new antifungals that are hydrophobic and highly potent have been introduced. Objectives To investigate the impact of the number of tip changes during serial dilution, and ISO and serial dilution for nine antifungals. Methods EUCAST E.Def 7.3.2 with serial (0–10 tip changes) and ISO dilution. Candida parapsilosis ATCC 22019, Candida albicans ATCC 64548, C. albicans CNM CL-F8555, Candida krusei ATCC 6258, Aspergillus flavus ATCC 204304 and clinical isolates (n = 5) of C. albicans, Candida dubliniensis, Candida glabrata, C. krusei, A. flavus and Aspergillus terreus were included. GM MICs were compared for ISO and serial dilution and with QC values where available. Results Increasing the number of tip changes (0/1/2/10 times) during serial dilution for plate preparation increased the MICs 1 to &gt;2 dilutions for amphotericin B, anidulafungin, micafungin, fluconazole, voriconazole and isavuconazole against C. albicans ATCC 64548 but only isavuconazole MICs against C. parapsilosis ATCC 22019 (3 dilutions). ISO and serial dilution (two tip changes) were compared for eight compounds and four Candida QC strains (352 MICs). Six/41 GM MIC pairs deviated with 1–1.8 dilution (14.6%). Comparing the GM MIC with the QC values, the ISO method GM MIC was closest to the target in 30.8%, the serial dilution in 34.6% and the methods identical in 34.6% of the cases. Finally, ISO and serial dilution MICs were compared for clinical isolates (920 MICs). Five/23 GM MIC pairs (21.7%) deviated 1.0–1.1 dilutions. Conclusions The ISO and serial dilution (two tip changes) method were in acceptable agreement and thus equally applicable for EUCAST testing.

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3

Blodgett, Robert J. "Planning a serial dilution test with multiple dilutions." Food Microbiology 26, no. 4 (2009): 421–24. http://dx.doi.org/10.1016/j.fm.2009.02.001.

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4

Paegel, Brian M., William H. Grover, Alison M. Skelley, Richard A. Mathies, and Gerald F. Joyce. "Microfluidic Serial Dilution Circuit." Analytical Chemistry 78, no. 21 (2006): 7522–27. http://dx.doi.org/10.1021/ac0608265.

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5

Ahrar, Siavash, Michelle Hwang, Philip N. Duncan, and Elliot E. Hui. "Microfluidic serial dilution ladder." Analyst 139, no. 1 (2014): 187–90. http://dx.doi.org/10.1039/c3an01710a.

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6

Harris, Cole O., and Stephanie L. Schweiker. "Optimizing production of serially diluted compounds and distribution to multiple targets." Journal of Automated Methods and Management in Chemistry 23, no. 6 (2001): 179–82. http://dx.doi.org/10.1155/s1463924601000220.

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The need for a multiple-target compound selectivity programme led to the establishment of a single robotic system that produces a compound's serial dilution and its distribution to multiple replicate assay plates. A Genesis RSP 150 integrated into a Zymate Laboratory Automation System XP produced the serial dilutions, and the subsequent replicate assay plates were produced quickly and accurately by an efficient use of the carousels and rapid plate. Currently, this process allows for the production of over 200 serial dilution assay plates in a workday.

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7

Flessland, Karen A., Helen R. Landicho, Kimberlee K. Borden, and Harry E. Prince. "Performance Characteristics of the PolyTiter Immunofluorescent Titration System for Determination of Antinuclear Antibody Endpoint Dilution." Clinical and Vaccine Immunology 9, no. 2 (2002): 329–32. http://dx.doi.org/10.1128/cdli.9.2.329-332.2002.

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ABSTRACT Conventional screening for circulating antinuclear antibodies (ANA) is generally performed by immunofluorescent (IF) microscopy with a 1:40 dilution of serum. Intensity of IF staining is then semiquantitated by using twofold serial dilutions, where the highest dilution in which staining intensity equals the endpoint control is expressed as an endpoint titer. The PolyTiter Immunofluorescent Titration system (Polymedco, Inc.) facilitates ANA-IF assay (IFA) testing by relating the intensity of IF staining to reference calibrators (defined in PolyTiter units), providing an endpoint titer directly from a 1:40 dilution. This study was conducted to assess the performance characteristics of the PolyTiter system. Two technologists each evaluated 10 replicates of three specimens and two controls on five sequential days. Endpoint dilution agreement (defined as ±2 dilutions) with the reference was 100% for all controls and for all specimens by one technologist. The second reader reported agreement of 98, 88, and 100% for the low, medium, and high specimens, respectively. Analysis of PolyTiter unit values yielded between-reader, between-run, and within-run precision coefficients of variation of less than 10%. The variance component in the lot-to-lot analysis was zero, indicating all of the variation was due to run-to-run differences. Overall endpoint dilution agreement between PolyTiter and serial dilution in the evaluation of 125 specimens at three sites was 90, 93, and 86%. Pattern identification with the PolyTiter was similar to that with serial dilution. The PolyTiter system demonstrates acceptable performance for routine ANA-IFA testing in the clinical laboratory.

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8

Gelman, Andrew, Ginger L. Chew, and Michael Shnaidman. "Bayesian Analysis of Serial Dilution Assays." Biometrics 60, no. 2 (2004): 407–17. http://dx.doi.org/10.1111/j.0006-341x.2004.00185.x.

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9

Ben-David, Avishai, and Charles E. Davidson. "Estimation method for serial dilution experiments." Journal of Microbiological Methods 107 (December 2014): 214–21. http://dx.doi.org/10.1016/j.mimet.2014.08.023.

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10

Gu, Guo-Yue, Yi-Wei Lee, Chih-Chung Chiang, and Ya-Tang Yang. "A nanoliter microfluidic serial dilution bioreactor." Biomicrofluidics 9, no. 4 (2015): 044126. http://dx.doi.org/10.1063/1.4929946.

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